Publications by authors named "Xiaowei Liu"

625 Publications

Effect of Pathogenic Fungal Infestation on the Berry Quality and Volatile Organic Compounds of Cabernet Sauvignon and Petit Manseng Grapes.

Front Plant Sci 2022 22;13:942487. Epub 2022 Jul 22.

College of Food Science and Technology, Hebei Agricultural University, Baoding, China.

The effect of pathogenic fungal infestation on berry quality and volatile organic compounds (VOCs) of Cabernet Sauvignon (CS) and Petit Manseng (PM) were investigated by using biochemical assays and gas chromatography-ion mobility spectrometry. No significant difference in diseases-affected grapes for 100-berry weight. The content of tannins and vitamin C decreased significantly in disease-affected grapes, mostly in white rot-affected PM, which decreased by 71.67% and 66.29%. The reduced total flavonoid content in diseases-affected grape, among which the least and most were anthracnose-affected PM (1.61%) and white rot-affected CS (44.74%). All diseases-affected CS had much higher titratable acid, a maximum (18.86 g/100 ml) was observed in the gray mold-affected grapes, while only anthracnose-affected grapes with a higher titratable acid level (21.8 g/100 mL) were observed in PM. A total of 61 VOCs were identified, including 14 alcohols, 13 esters, 12 aldehydes, 4 acids, 4 ketones, 1 ether, and 13 unknown compounds, which were discussed from different functional groups, such as C6-VOCs, alcohols, ester acetates, aldehydes, and acids. The VOCs of CS changed more than that of Petit Manseng's after infection, while gray mold-affected Cabernet Sauvignon had the most change. C6-VOCs, including hexanal and ()-2-hexenal were decreased in all affected grapes. Some unique VOCs may serve as hypothetical biomarkers to help us identify specific varieties of pathogenic fungal infestation.
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http://dx.doi.org/10.3389/fpls.2022.942487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353940PMC
July 2022

Platelet TGF-β1 inhibits the migration and proliferation of smooth muscle cells in aneurysms.

Cytokine 2022 Aug 2;158:155969. Epub 2022 Aug 2.

Department of Cardiology, Taizhou Hospital of Wenzhou Medical University, China; Department of Radiation Oncology, Indiana University School of Medicine, United States. Electronic address:

Background: The study explored the role of platelet TGF-β1 from the perspective of inhibiting the excessive proliferation, migration and invasion of murine aortic vascular smooth muscle cells (MASMCs).

Method: The platelets were first extracted from C57BL/6 mice, and the TGF-β1 protein was obtained after the purification of protein. In vitro, the concentrations of angiotensin Ⅱ (Ang Ⅱ) and TGF-β1 for intervention were screened by testing the viability of MASMCs, followed by the analysis concerning the effects of platelets, Ang Ⅱ and TGF-β1 on the proliferation, migration, invasion, and the expressions of pathway-related proteins in MASMCs. In vivo, an Ang Ⅱ-induced mouse model was established. TGF-β1 was injected into the tail of mice as a therapeutic agent, and its action mechanism was further verified by the treatment of inhibitor SB505124. The results of the cell experiment were validated by evaluating the maximum diameter of abdominal aorta, the proportion of total weight, the changes of both pathology and the expressions of pathway-related proteins in the mice.

Result: 0.5 ng/mL Ang Ⅱ and 15 ng/mL TGF-β1 were chosen for treatment. The following results of cell functional experiments and Western blot assay demonstrated that Ang Ⅱ promoted the proliferation, migration and invasion of MASMCs via regulating related pathways, the effects of which were evidently reversed by TGF-β1 and platelets. Consistent results were also observed in the animal experiments, where TGF-β1 effectively alleviated Ang Ⅱ-induced abdominal aortic injury in mice.

Conclusion: TGF-β1 in platelets inhibits Ang Ⅱ-induced proliferation, migration and invasion of MASMCs.
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http://dx.doi.org/10.1016/j.cyto.2022.155969DOI Listing
August 2022

Bicyclization of 4-Cyano-1,2-diketones with Amidines: Synthesis of Tetrahydroimidazo[4,5-]-5-pyridinones.

J Org Chem 2022 Aug 4. Epub 2022 Aug 4.

Jilin Province Key Laboratory of Organic Functional Molecular Design and Synthesis, College of Chemistry, Northeast Normal University, Changchun, Jilin 130024, China.

A new bicyclization of 4-cyano-1,2-diketones with amidines is reported for the construction of tetrahydro-5-imidazo[4,5-]pyridin-5-ones. The tandem reaction is proposed to involve the double condensation-cyclization of diketone with amidine, an α-ketol like rearrangement, the hydrolysis of nitrile followed by intramolecular nucleophilic addition, and the elimination of water. Features of the bicyclization method include high yields, good functional group tolerance, and mild reaction conditions.
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http://dx.doi.org/10.1021/acs.joc.2c01466DOI Listing
August 2022

A novel strategy to fuel cancer immunotherapy: targeting glucose metabolism to remodel the tumor microenvironment.

Front Oncol 2022 18;12:931104. Epub 2022 Jul 18.

Laboratory of Integrative Medicine, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

The promising results of immunotherapy in tumors have changed the current treatment modality for cancer. However, the remarkable responses are limited to a minority of patients, which is due to immune suppression in the tumor microenvironment (TME). These include the pre-exists of suppressive immune cells, physical barriers to immune infiltration, antigen and antigen presentation deficiency, and expression of inhibitory immune checkpoint molecules. Recently, increasing evidence reveal that tumor metabolism, especially abnormal glucose metabolism of tumors, plays an essential role in tumor immune escape and is a potential target to combine with immunotherapy. By glucose uptake, tumor cells alter their metabolism to facilitate unregulated cellular proliferation and survival and regulate the expression of inhibitory immune checkpoint molecules. Meanwhile, glucose metabolism also regulates the activation, differentiation, and functions of immunocytes. In addition, tumor mainly utilizes glycolysis for energy generation and cellular proliferation, which cause the TME to deplete nutrients for infiltrating immune cells such as T cells and produce immunosuppressive metabolites. Thus, therapeutics that target glucose metabolism, such as inhibiting glycolytic activity, alleviating hypoxia, and targeting lactate, have shown promise as combination therapies for different types of cancer. In this review, we summarized the functions of glucose metabolism in the tumor cells, immune cells, and tumor microenvironment, as well as strategies to target glucose metabolism in combination with immune checkpoint blockade for tumor therapy.
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http://dx.doi.org/10.3389/fonc.2022.931104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340371PMC
July 2022

Environmental behavior of the chiral fungicide epoxiconazole in earthworm-soil system: Enantioselective enrichment, degradation kinetics, chiral metabolite identification, and biotransformation mechanism.

Environ Int 2022 Jul 30;167:107442. Epub 2022 Jul 30.

Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, PR China. Electronic address:

The environmental impact of the chiral fungicide epoxiconazole and its chiral transformation products (TPs) on non-target organisms and the environment has become a significant concern due to its widespread use in agricultural practice. Enantioselectivity studies of parent contaminants cannot adequately assess the complexity of its chiral TPs in the environment. This study aimed to investigate the environmental behavior of epoxiconazole in an earthworm-soil system. 2S,3R-(-)-epoxiconazole was preferentially enriched in earthworms during the accumulation phase (p < 0.05), but no enantioselectivity was observed during the elimination phase. One methoxylated and four hydroxylated chiral TPs were identified in soil, earthworm, and excrement. The epoxy ring hydroxylated TP and methoxylated TP of epoxiconazole were discovered for the first time in the environment. The chemically specific enantioselectivity with enantiomer fraction (EF) > 0.8 was observed for the TPs in different matrices. The CYP450 monooxygenase of earthworm was significant activated. In vitro enzyme metabolism experiments (earthworm microsomes and recombinant CYP450 enzymes CYP2A6, CYP 2C9, and CYP 3A4) were carried out to further explain the biotransformation mechanism of epoxiconazole in earthworm. This study provides new evidence of enantiomeric biotransformation of chiral fungicide epoxiconazole in the earthworm-soil system and could provide valuable insights into their environmental risk assessment.
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http://dx.doi.org/10.1016/j.envint.2022.107442DOI Listing
July 2022

Lactate Metabolism-Associated lncRNA Pairs: A Prognostic Signature to Reveal the Immunological Landscape and Mediate Therapeutic Response in Patients With Colon Adenocarcinoma.

Front Immunol 2022 11;13:881359. Epub 2022 Jul 11.

Department of Gastroenterology, Affiliated Hospital of Xiangnan University, Chenzhou, China.

Background: Lactate metabolism is critically involved in the tumor microenvironment (TME), as well as cancer progression. It is important to note, however, that lactate metabolism-related long non-coding RNAs (laRlncRNAs) remain incredibly understudied in colon adenocarcinoma (COAD).

Methods: A gene expression profile was obtained from the Cancer Genome Atlas (TCGA) database to identify laRlncRNA expression in COAD patients. A risk signature with prognostic value was identified from TCGA and Gene Expression Omnibus (GEO) cohort based on laRlncRNA pairs by the least absolute shrinkage and selection operator (LASSO) and Cox regression analyses. Quantitative real-time polymerase chain reaction (qRT-PCR) and functional experiments were carried out to verify the expression of laRlncRNAs in COAD. The relationship of laRlncRNA pairs with immune landscape as well as the sensitivity of different therapies was explored.

Results: In total, 2378 laRlncRNAs were identified, 1,120 pairs of which were studied to determine their prognostic validity, followed by a risk signature established based on the screened 5 laRlncRNA pairs. The laRlncRNA pairs-based signature provided a better overall survival (OS) prediction than other published signatures and functioned as a prognostic marker for COAD patients. According to the calculated optimal cut-off point, patients were divided into high- and low-risk groups. The OS of COAD patients in the high-risk group were significantly shorter than that of those in the low-risk group (P=4.252e-14 in the TCGA cohort and P=2.865-02 in the GEO cohort). Furthermore, it remained an effective predictor of survival in strata of gender, age, TNM stage, and its significance persisted after univariate and multivariate Cox regressions. Additionally, the risk signature was significantly correlated with immune cells infiltration, tumor mutation burden (TMB), microsatellite instability (MSI) as well as immunotherapeutic efficacy and chemotherapy sensitivity. Finally, one of the laRlncRNA, LINC01315, promotes proliferation and migration capacities of colon cancer cells.

Conclusion: The newly identified laRlncRNAs pairs-based signature exhibits potential effects in predicting prognosis, deciphering patients' immune landscape, and mediating sensitivity to immunotherapy and chemotherapy. Findings in our study may provide evidence for the role of laRlncRNAs pairs as novel prognostic biomarkers and potentially individualized therapy targets for COAD patients.
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http://dx.doi.org/10.3389/fimmu.2022.881359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328180PMC
July 2022

Scalable fabrication of Solvent-Free composite solid electrolyte by a continuous Thermal-Extrusion process.

J Colloid Interface Sci 2022 Jul 19;628(Pt A):64-71. Epub 2022 Jul 19.

Advanced Membranes and Porous Materials Centre, Chemical Engineering Program, Division of Physical Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia. Electronic address:

Composite solid-state electrolytes (CSEs) are regarded as a promising alternative for the next-generation lithium-ion batteries because they integrate the advantages of inorganic electrolytes and organic electrolytes. However, there are two issues faced by current CSEs: 1) a green and feasible approach to prepare CSEs in large scales is desired; and 2) the trace solvents, remaining from the preparation processes, lead to some serious concerns, such as safety hazard issues, electrolyte-electrode interfacial issues, and reduced durability of batteries. Here, a continuous thermal-extrusion process is presented to realize the large-scale fabrication of solvent-free CSE. A 38.7-meter CSE membrane was prepared as a demonstration in this study. Thanks to the elimination of residual solvents, the electrolyte membrane exhibited a high tensile strength of 3.85 MPa, satisfactory lithium transference number (0.495), and excellent electrochemical stability (5.15 V). Excellent long-term stability was demonstrated by operating the symmetric lithium cell at a stable current density of 0.1 mA cm for over 3700 h. Solvent-free CSE lithium metal batteries showed a discharge capacity of 155.7 - 25.17 mAh g at 0.1 - 2.0C, and the discharge capacity remained 78.1% after testing for 380cycles.
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http://dx.doi.org/10.1016/j.jcis.2022.07.099DOI Listing
July 2022

Removal of Carbamazepine in Aqueous Solution by CoS/Fe/PMS Process.

Molecules 2022 Jul 15;27(14). Epub 2022 Jul 15.

Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.

Carbamazepine (CBZ), as a typical pharmaceutical and personal care product (PPCP), cannot be efficiently removed by the conventional drinking water and wastewater treatment process. In this work, the CoS/Fe/PMS process was applied for efficient elimination of CBZ. The CBZ removal efficiency of CoS/Fe/PMS was 2.5 times and 23 times higher than that of CoS/PMS and Fe/PMS, respectively. The intensity of DMPO-HO• and DMPO-SO4•- followed the order of Fe/PMS < CoS/PMS < CoS/Fe/PMS, also suggesting the CoS/Fe/PMS process has the highest oxidation activity. The effects of reaction conditions (e.g., CoS dosage, Fe concentration, PMS concentration, initial CBZ concentration, pH, temperature) and water quality parameters (e.g., SO42-, NO3-, H2PO4-, Cl-, NH4+, humic acid) on the degradation of CBZ were also studied. Response surface methodology analysis was carried out to obtain the best conditions for the removal of CBZ, which are: Fe = 70 µmol/L, PMS = 240 µmol/L, CoS = 0.59 g/L. The sustainability test demonstrated that the repeated use of CoS for 8 successive cycles resulted in little function decrease (<10%). These findings suggest that CoS/Fe/PMS may be a promising method for advanced treatment of tailwater from sewage treatment plant.
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http://dx.doi.org/10.3390/molecules27144524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9323623PMC
July 2022

Simultaneous determination of succinate-dehydrogenase-inhibitor fungicide traces in cereals by QuEChERS preparation and UPLC-MS/MS analysis.

Food Chem 2022 Jul 16;396:133708. Epub 2022 Jul 16.

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. Electronic address:

A method for the simultaneous determination of 19 succinate dehydrogenase inhibitor (SDHI) fungicide residues in 8 kinds of cereals was established by combining UHPLC-MS/MS with the improved QuEChERS method. MgSO and octadecylsilane (C) were used as the dispersive-solid phase extraction sorbent. The proposed method had good linearity in the range of 10-100 µg/L with correlation coefficients (R > 0.99). The limit of quantification of 19 fungicides was 10 µg/L, which is the minimum addition level of the method. The fortified recoveries of 19 SDHI fungicides at three levels were ranged from 79.57 % to 126.25 %. The developed method was utilized for the analysis of 45 real cereal samples, only 5 samples were detected with SDHI fungicides. The contents of the fungicides detected in the real samples are far lower than the MRL. The results indicated that the proposed method is reliable for detecting SDHI fungicides in cereals.
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http://dx.doi.org/10.1016/j.foodchem.2022.133708DOI Listing
July 2022

The influence of wind-induced sediment resuspension and migration on raw water turbidity in Lake Taihu, China.

Environ Sci Pollut Res Int 2022 Jul 4. Epub 2022 Jul 4.

College of Civil Engineering and Architecture, Zhejiang University, 866# Yuhangtang Road Hangzhou, 310058, Zhejiang, People's Republic of China.

Lake Taihu is an important drinking water source for cities in the Yangtze River Delta region, while the dramatic fluctuation in turbidity has caused severe problems for local waterwork management. The effects of sediment resuspension and migration on turbidity of water intake in a waterworks located in the south of Lake Taihu are studied. Because the sediment behavior induced by wind disturbance was believed to be the main factor for the matter, the effect of wind field on the hydrodynamics and sediment distribution of Lake Taihu was conducted based on a wind-driven numerical model. The obtained results indicate that wind direction was more influential on the structure of flow and wave fields, while wind speed contributed more to the field intensity. The suspended sediment concentration (SSC) of water intake area was most sensitive to onshore winds, which led to significant increase in suspended sediment concentration at 3 m/s, while for alongshore winds, the incipient speed was 4 m/s, and offshore winds were less influential. In addition, the suspended sediment of the water intake area was primarily migrated from other erosion regions rather than local suspended particles. A 7-h lag was found between SSC and the measured turbidity confirmed the lag effect of wind disturbance on turbidity change. The high consistency between the 2 series demonstrated the potential of this method to turbidity prediction when combined with the weather forecast technique.
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http://dx.doi.org/10.1007/s11356-022-21724-7DOI Listing
July 2022

Objective Outcomes of an Extended Anti-reflux Mucosectomy in the Treatment of PPI-Dependent Gastroesophageal Reflux Disease (with Video).

J Gastrointest Surg 2022 Jul 1. Epub 2022 Jul 1.

Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Background: Anti-reflux mucosectomy (ARMS) is a choice for proton pump inhibitor (PPI)-dependent patients with gastroesophageal reflux disease (GERD). We present an extended anti-reflux mucosectomy, named ligation-assisted anti-reflux mucosectomy (L-ARMS). The aim of this study was to assess the feasibility of the procedure and short-term outcomes on PPI use and symptom resolution.

Methods: Institutional review board approval was obtained for retrospective review of a prospectively collected database including patients who underwent L-ARMS. L-ARMS includes mucosa ligation and endoscopic mucosectomy without submucosal injection around the squamocolumnar junction. The GERD symptoms, endoscopy, 24-h pH monitoring results, and manometry were collected by chart review. Voluntary validated surveys assessed symptomatic improvement over time.

Results: There were 69 patients available for review. The procedure was technically completed in all cases with no severe complications, and the average operation time was 33 min. At 6 months after L-ARMS, treatment with PPIs had been halted in 55.1% of the patients, 30.4% of the enrolled patients used PPIs occasionally, and the lower esophageal sphincter (LES) pressure, DeMeester scores, and GERD-health-related quality of life questionnaire (GERD-HRQL) scores showed a significant improvement compared with the baseline measurements (P < 0.001). Forty-five patients complained of mild dysphagia and were relieved in 4 weeks with no specific treatment. Compared to patients without dysphagia, patients complained of dysphagia after surgery had better clinical benefits indicated by GERD-HRQL and DeMeester score.

Conclusions: As a modified ARMS, L-ARMS is an effective procedure for controlling GERD symptoms, esophageal acid exposure, and LES pressure, which can be safely performed endoscopically in a time-saving and simple manner.
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http://dx.doi.org/10.1007/s11605-022-05396-9DOI Listing
July 2022

Giant Osmotic Energy Conversion through Vertical-Aligned Ion-Permselective Nanochannels in Covalent Organic Framework Membranes.

J Am Chem Soc 2022 Jul 28;144(27):12400-12409. Epub 2022 Jun 28.

Division of Physical Science and Engineering, 4700 King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia.

Nanofluidic membranes have been demonstrated as promising candidates for osmotic energy harvesting. However, it remains a long-standing challenge to fabricate high-efficiency ion-permselective membranes with well-defined channel architectures. Here, we demonstrate high-performance osmotic energy conversion membranes based on oriented two-dimensional covalent organic frameworks (COFs) with ultrashort vertically aligned nanofluidic channels that enabled efficient and selective ion transport. Experiments combined with molecular dynamics simulations revealed that exquisite control over channel orientation, charge polarity, and charge density contributed to high ion selectivity and permeability. When applied to osmotic energy conversion, a pair of 100 nm thick oppositely charged COF membranes achieved an ultrahigh output power density of 43.2 W m at a 50-fold salinity gradient and up to 228.9 W m for the Dead Sea and river water system. The achieved power density outperforms the state-of-the-art nanofluidic membranes, suggesting the great potential of oriented COF membranes in the fields of advanced membrane technology and energy conversion.
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http://dx.doi.org/10.1021/jacs.2c04223DOI Listing
July 2022

Elevated CO Altered Rice VOCs Aggravate Population Occurrence of Brown Planthoppers by Improving Host Selection Ability.

Biology (Basel) 2022 Jun 8;11(6). Epub 2022 Jun 8.

Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

It is predicted that plant volatile organic compounds (VOCs) are affected by the atmospheric CO levels rising globally, which further affects the interaction between plants and herbivorous insects, especially the host selection behavior of herbivorous insects. In this study, the effects of elevated CO on the host-selection behavior of the brown planthopper (BPH) , and the emission of VOCs from the healthy and BPH-damaged rice plants were studied simultaneously to make clear the population occurrence of BPH under global climate change. Compared with ambient CO, elevated CO significantly increased the host selection percent of BPH for the healthy (CK) and BPH-damaged rice plants, and the host selection percent of BPH for the BPH-damaged rice plants was significantly higher than that for the healthy rice plants under elevated CO, which might be regulated by the transcription levels of , and in BPH due to the upregulated transcriptional levels of these three genes of BPH under elevated CO. In addition, we analyzed and quantified the emission of VOCs in rice plants grown under ambient CO and elevated CO by GS-MS. A total of 36 VOCs from rice plants were identified into eight categories, including alkanes, alkenes, alcohols, aldehydes, ketones, esters, phenols and aromatic hydrocarbons. Elevated CO significantly decreased the contents of heptadecane, linalool and limonene from rice plants compared with ambient CO. Besides, the contents of linalool, phytol, decanal, 1-methyldecalin and 2,6-diphenylphenol from BPH-damaged rice plants under ambient CO, and undecane, hexadecane, nonanal and 2,6-diphenylphenol from BPH-damaged rice plants under elevated CO were all significantly higher than those from healthy rice plants. The percentage composition of phenols was positively correlated with the host selection rate of BPH. Our study indicates that elevated CO is beneficial to promote the host selection ability of BPH for rice plants damaged by BPHs due to the changed plant VOCs.
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http://dx.doi.org/10.3390/biology11060882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219841PMC
June 2022

Plant Diversity and Fungal Richness Regulate the Changes in Soil Multifunctionality in a Semi-Arid Grassland.

Biology (Basel) 2022 Jun 6;11(6). Epub 2022 Jun 6.

Key Laboratory of Vegetation Ecology, Institute of Grassland Science, Northeast Normal University, Ministry of Education, Changchun 130024, China.

Loss in plant diversity is expected to impact biodiversity and ecosystem functioning (BEF) in terrestrial ecosystems. Soil microbes play essential roles in regulating ecosystem functions. However, the important roles and differences in bacterial and fungal diversity and rare microbial taxa in driving soil multifunctionality based on plant diversity remain poorly understood in grassland ecosystems. Here, we carried out an experiment in six study sites with varied plant diversity levels to evaluate the relationships between soil bacterial and fungal diversity, rare taxa, and soil multifunctionality in a semi-arid grassland. We used Illumina HiSeq sequencing to determine soil bacterial and fungal diversity and evaluated soil functions associated with the nutrient cycle. We found that high diversity plant assemblages had a higher ratio of below-ground biomass to above-ground biomass, soil multifunctionality, and lower microbial carbon limitation than those with low diversity. Moreover, the fungal richness was negatively and significantly associated with microbial carbon limitations. The fungal richness was positively related to soil multifunctionality, but the bacterial richness was not. We also found that the relative abundance of saprotrophs was positively correlated with soil multifunctionality, and the relative abundance of pathogens was negatively correlated with soil multifunctionality. In addition, the rare fungal taxa played a disproportionate role in regulating soil multifunctionality. Structural equation modeling showed that the shift of plant biomass allocation patterns increased plant below-ground biomass in the highly diverse plant plots, which can alleviate soil microbial carbon limitations and enhance the fungal richness, thus promoting soil multifunctionality. Overall, these findings expand our comprehensive understanding of the critical role of soil fungal diversity and rare taxa in regulating soil multifunctionality under global plant diversity loss scenarios.
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http://dx.doi.org/10.3390/biology11060870DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220314PMC
June 2022

Development of bacterial resistance induced by low concentration of two-dimensional black phosphorus mutagenesis.

RSC Adv 2022 May 30;12(25):16071-16078. Epub 2022 May 30.

School of Biology, Food, and Environment, Hefei University Hefei 230601 China

The wide use of nano-antibacterial materials has triggered concerns over the development of nanomaterials-associated bacterial resistance. Two-dimensional (2D) black phosphorus (BP) as a new class of emerging 2D nanomaterial has displayed excellent antibacterial performance. However, whether bacteria repeatedly exposed to 2D BP can develop resistance is not clear. We found that wild type K-12 MG 1655 strains can increase resistance to 2D-BP nanosheets after repeated exposure with subinhibitory concentration of 2D-BP nanosheets. Adaptive morphogenesis including the reinforced barrier function of cell membrane were observed in the resistant bacteria, which enhanced the resistance of bacteria to 2D-BP nanosheets. The whole-genome sequencing analysis showed that the three mutation genes including , , and genes were observed in the 2D-BP resistant strains, which controlled catabolism, membrane structure, and DNA replication, respectively. Furthermore, transcriptional sequencing confirmed that these genes related to metabolization, membrane structure, and cell motility were upregulated in the 2D-BP resistant bacteria. The development of resistance to 2D-BP in bacteria mainly attributed to the changes in energy metabolism and membrane structure of bacteria caused by gene mutations. In addition, the up-regulated function of cell motility also helped the bacteria to develop resistance by escaping external stimuli. The results provided new evidence for understanding an important effect of nano-antibacterial materials on the development of bacterial resistance.
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http://dx.doi.org/10.1039/d2ra01263dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9149864PMC
May 2022

Wee1 kinase inhibitor adavosertib with radiation in newly diagnosed diffuse intrinsic pontine glioma: A Children's Oncology Group phase I consortium study.

Neurooncol Adv 2022 Jan-Dec;4(1):vdac073. Epub 2022 May 20.

Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota.

Background: Children with diffuse intrinsic pontine gliomas (DIPG) have a dismal prognosis. Adavosertib (AZD1775) is an orally available, blood-brain barrier penetrant, Wee1 kinase inhibitor. Preclinical efficacy against DIPG is heightened by radiation induced replication stress.

Methods: Using a rolling six design, 7 adavosertib dose levels (DLs) (50 mg/m alternating weeks, 50 mg/m alternating with weeks of every other day, 50 mg/m, then 95, 130, 160, 200 mg/m) were assessed. Adavosertib was only given on days of cranial radiation therapy (CRT).The duration of CRT (54 Gy over 30 fractions; 6 weeks) constituted the dose limiting toxicity (DLT) period. Endpoints included tolerability, pharmacokinetics, overall survival (OS) and peripheral blood γH2AX levels as a marker of DNA damage.

Results: A total of 46 eligible patients with newly diagnosed DIPG [median (range) age 6 (3-21) years; 52% female] were enrolled. The recommend phase 2 dose (RP2D) of adavosertib was 200 mg/m/d during days of CRT. Dose limiting toxicity included ALT elevation (n = 1, DL4) and neutropenia (n = 1, DL7). The mean T, T and Cl on Day 1 were 2 h, 4.4 h, and 45.2 L/hr/m, respectively. Modest accumulation of adavosertib was observed comparing day 5 versus day 1 AUC (accumulation ratio = 1.6). OS was 11.1 months (95% CI: 9.4, 12.5) and did not differ from historical control.

Conclusion: Adavosertib in combination with CRT is well tolerated in children with newly diagnosed DIPG, however, compared to historical controls, did not improve OS. These results can inform future trial design in children with high-risk cancer.
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http://dx.doi.org/10.1093/noajnl/vdac073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209747PMC
May 2022

A Machine Learning-Based System for Real-Time Polyp Detection (DeFrame): A Retrospective Study.

Front Med (Lausanne) 2022 31;9:852553. Epub 2022 May 31.

Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha, China.

Background And Aims: Recent studies have shown that artificial intelligence-based computer-aided detection systems possess great potential in reducing the heterogeneous performance of doctors during endoscopy. However, most existing studies are based on high-quality static images available in open-source databases with relatively small data volumes, and, hence, are not applicable for routine clinical practice. This research aims to integrate multiple deep learning algorithms and develop a system (DeFrame) that can be used to accurately detect intestinal polyps in real time during clinical endoscopy.

Methods: A total of 681 colonoscopy videos were collected for retrospective analysis at Xiangya Hospital of Central South University from June 2019 to June 2020. To train the machine learning (ML)-based system, 6,833 images were extracted from 48 collected videos, and 1,544 images were collected from public datasets. The DeFrame system was further validated with two datasets, consisting of 24,486 images extracted from 176 collected videos and 12,283 images extracted from 259 collected videos. The remaining 198 collected full-length videos were used for the final test of the system. The measurement metrics were sensitivity and specificity in validation dataset 1, precision, recall and F1 score in validation dataset 2, and the overall performance when tested in the complete video perspective.

Results: A sensitivity and specificity of 79.54 and 95.83%, respectively, was obtained for the DeFrame system for detecting intestinal polyps. The recall and precision of the system for polyp detection were determined to be 95.43 and 92.12%, respectively. When tested using full colonoscopy videos, the system achieved a recall of 100% and precision of 80.80%.

Conclusion: We have developed a fast, accurate, and reliable DeFrame system for detecting polyps, which, to some extent, is feasible for use in routine clinical practice.
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http://dx.doi.org/10.3389/fmed.2022.852553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9194608PMC
May 2022

Phase Angle as a Comprehensive Tool for Nutritional Monitoring and Management in Patients with Crohn's Disease.

Nutrients 2022 May 28;14(11). Epub 2022 May 28.

Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410008, China.

Background And Aims: Crohn's disease (CD) is usually accompanied by malnutrition. CD-related malnutrition can increase morbidity, disability, mortality, and hospitalization costs. The purpose of this study was to find a reliable indicator for evaluating CD patients' nutritional status.

Methods: All data were retrospectively collected from Xiangya Hospital, Central South University between May 2021 and February 2022. All patients were evaluated for nutritional status using the Global Leadership Initiative on Malnutrition (GLIM) criteria. Body composition, resistance, and reactance were recorded by a body analyser, and the phase angle (PhA) was calculated simultaneously. The Mann-Whitney U test, chi-square test, Fisher's exact test, and univariate and multivariate logistic regression analyses were used. A receiver operating characteristic (ROC) curve was built to evaluate the predictive value of differential variables for diagnosing malnutrition based on the GLIM criteria.

Results: A total of 169 CD patients were enrolled, of which 74 (58.3%) males and 32 (76.2%) females were diagnosed with malnutrition; 34 (45.9%) males and 22 (68.8%) females were severely malnourished. Univariate analysis identified that as nutritional status deteriorated, body mass index, PhA, and levels of haemoglobin and albumin decreased, while platelet count, erythrocyte sedimentation rate, and levels of C-reactive protein and fibrinogen increased ( < 0.05). Logistic regression analysis revealed that the PhA was significantly independently associated with malnutrition ( < 0.05). The ROC curve analysis indicated that the optimal PhA cut-off levels of 6.11° and 5.55° could be used to predict malnutrition according to the GLIM criteria in males and females, respectively, with a PhA < 5.53° and < 5.12° indicating severe malnutrition in males and females, respectively.

Conclusion: The PhA is a sensitive, noninvasive, portable, inexpensive tool that can be used to monitor and manage the nutritional status of CD patients.
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http://dx.doi.org/10.3390/nu14112260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9182801PMC
May 2022

Comprehensive Analysis of the Expression and Prognosis for the DREAM Complex in Human Cancers.

Front Genet 2022 12;13:814725. Epub 2022 May 12.

Department of Pediatric Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

The DREAM complex is an evolutionarily conserved cell cycle regulating multi-protein complex. In addition to playing an essential function in the cell cycle, it also plays a vital role in various survival activities. Accumulating evidence suggests that the DREAM complex plays a crucial role in oncogenesis. However, the regulatory mechanism of the DREAM complex in cancer remains unclear. This study used multi-omics data from Cancer Genome Atlas and Cancer Cell Line Encyclopedia to comprehensively identify the DREAM complex in tumor samples from 33 cancer types. In the genomic landscape, we identified the missense mutation as the dominant alteration events. Expression analysis showed that the expression of methylation-mediated the DREAM complex was downregulated. In addition, we found that the expression of the DREAM complex can be performed to predict the survival of various cancer patients. Pathway activation analysis showed that the DREAM complex is related to apoptosis inhibition, cell cycle, DNA damage response, RAS/MAPK, and RTK signaling pathway activation. Importantly, through a comprehensive analysis of drug sensitivity genomics in cancer databases, we identified a number of potential drugs that may target the DREAM complex. In summary, this study revealed the genomic changes and clinical features of the DREAM complex in 33 cancers, which may also provide new insights for cancer treatment and may offer alternative options for the treatment of clinically refractory cancers.
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http://dx.doi.org/10.3389/fgene.2022.814725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161822PMC
May 2022

Brown Adipose Tissue and Novel Management Strategies for Polycystic Ovary Syndrome Therapy.

Front Endocrinol (Lausanne) 2022 19;13:847249. Epub 2022 May 19.

Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Beijing Maternal and Child Health Care Hospital, Capital Medical University, Beijing, China.

Brown adipose tissue (BAT), a unique tissue, plays a key role in metabolism and energy expenditure through adaptive nonshivering thermogenesis. It has recently become a therapeutic target in the treatment of obesity and metabolic diseases. The thermogenic effect of BAT occurs through uncoupling protein-1 by uncoupling adenosine triphosphate (ATP) synthesis from energy substrate oxidation. The review discusses the recent developments and progress associated with the biology, function, and activation of BAT, with a focus on its therapeutic potential for the treatment of polycystic ovary syndrome (PCOS). The endocrine activity of brown adipocytes affects the energy balance and homeostasis of glucose and lipids, thereby affecting the association of BAT activity and the metabolic profile. PCOS is a complex reproductive and metabolic disorder of reproductive-age women. Functional abnormalities of adipose tissue (AT) have been reported in patients with PCOS. Numerous studies have shown that BAT could regulate the features of PCOS and that increases in BAT mass or activity were effective in the treatment of PCOS through approaches including cold stimulation, BAT transplantation and compound activation in various animal models. Therefore, BAT may be used as a novel management strategy for the patients with PCOS to improve women's health clinically. It is highly important to identify key brown adipokines for the discovery and development of novel candidates to establish an efficacious therapeutic strategy for patients with PCOS in the future.
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http://dx.doi.org/10.3389/fendo.2022.847249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160465PMC
June 2022

Placental DNA Methylation Abnormalities in Prenatal Conotruncal Heart Defects.

Front Genet 2022 13;13:878063. Epub 2022 May 13.

Echocardiography Medical Center, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

This study aims to characterize the abnormal changes in placental DNA methylation associated with conotruncal heart defects (CTDs) and the level of methylation as epigenetic biomarkers for CTDs detection. This was a prospective study involving 28 fetuses diagnosed with CTDs in the second trimester at Beijing Anzhen Hospital between September 2020 and June 2021. These cases were classified into four groups based on their subtypes. 12 normal fetuses were used as controls. Placental tissue was obtained after inducing labor in fetuses. To identify differential methylation sites (DMSs) and regions (DMRs) in cases vs. controls, an Infinium Human Methylation 850 k bead chip was used. Differential methylation was assessed by comparing the β-values for individual CpG loci. Based on the p-value (<0.05), the most discriminating CpG sites were identified. The area under the receiver-operating-characteristics curve (AUC) was used to determine the predictive accuracy of CpG loci with significant methylation changes for CTDs. The function of genes was assessed through KEGG enrichment analysis, Gene Ontology (GO) analysis, and KEGG pathway analysis. In comparison to the control group, the DNA methylation of the placental tissue is significantly different in fetuses with CTDs. We identified the most significantly different methylated loci and they demonstrated excellent individual predictive accuracy for CTDs detection with AUC >0.9 in cases compared with controls. , and were identified as CTDs-detection candidate genes. Our study established the abnormal changes in placental methylation associated with CTDs and potential epigenetic biomarkers for CTDs detection.
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http://dx.doi.org/10.3389/fgene.2022.878063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9139681PMC
May 2022

Soil Microbial Legacy Overrides the Responses of a Dominant Grass and Nitrogen-Cycling Functional Microbes in Grassland Soil to Nitrogen Addition.

Plants (Basel) 2022 May 13;11(10). Epub 2022 May 13.

Key Laboratory of Vegetation Ecology, Ministry of Education, Jilin Songnen Grassland Ecosystem National Observation and Research Station, Institute of Grassland Science, Northeast Normal University, Changchun 130024, China.

Both atmospheric nitrogen (N) deposition and soil microbial legacy (SML) can affect plant performance, the activity of soil N-cycling functional microbes and the relative abundance of N-cycling functional genes (NCFGs). In the grassland vegetation successional process, how the interaction of SML and N deposition affects the performance of dominant grass and NCFGs remains unclear. Therefore, we planted , a dominant grass in the Songnen grassland, in the soil taken from the early, medium, late, and stable successional stages. We subjected the plants to soil sterilization and N addition treatments and measured the plant traits and NCFG abundances (i.e., , AOB , , and ). Our results showed the biomass and ramet number of in sterilized soil were significantly higher than those in non-sterilized soil, indicating that SML negatively affects the growth of . However, N addition increased the plant biomass and the AOB gene abundance only in sterilized soils, implying that SML overrode the N addition effects because SML buffered the effects of increasing soil N availability on NCFGs. Therefore, we emphasize the potential role of SML in assessing the effects of N deposition on dominant plant performance and NCFGs in the grassland vegetation succession.
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http://dx.doi.org/10.3390/plants11101305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9145027PMC
May 2022

A Low-FODMAP Diet Provides Benefits for Functional Gastrointestinal Symptoms but Not for Improving Stool Consistency and Mucosal Inflammation in IBD: A Systematic Review and Meta-Analysis.

Nutrients 2022 May 15;14(10). Epub 2022 May 15.

Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha 410008, China.

Background: A low fermentable oligosaccharides, disaccharides, monosaccharides, and polyols diet (LFD) is claimed to improve functional gastrointestinal symptoms (FGSs). However, the role of LFD in inflammatory bowel disease (IBD) patients with FGSs remains unclear.

Objective: To systematically assess the efficacy of LFD in IBD patients with FGSs.

Methods: Six databases were searched from inception to 1 January 2022. Data were synthesized as the relative risk of symptoms improvement and normal stool consistency, mean difference of Bristol Stool Form Scale (BSFS), Short IBD Questionnaire (SIBDQ), IBS Quality of Life (IBS-QoL), Harvey-Bradshaw index (HBi), Mayo score, and fecal calprotectin (FC). Risk of bias was assessed based on study types. A funnel plot and Egger's test were used to analyze publication bias.

Results: This review screened and included nine eligible studies, including four randomized controlled trials (RCTs) and five before-after studies, involving a total of 446 participants (351 patients with LFD vs. 95 controls). LFD alleviated overall FGSs (RR: 0.47, 95% CI: 0.33-0.66, = 0.0000) and obtained higher SIBDQ scores (MD = 11.24, 95% CI 6.61 to 15.87, = 0.0000) and lower HBi score of Crohn's disease (MD = -1.09, 95% CI -1.77 to -0.42, = 0.002). However, there were no statistically significant differences in normal stool consistency, BSFS, IBS-QoL, Mayo score of ulcerative colitis, and FC. No publication bias was found.

Conclusions: LFD provides a benefit in FGSs and QoL but not for improving stool consistency and mucosal inflammation in IBD patients. Further well-designed RCTs are needed to develop the optimal LFD strategy for IBD.
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http://dx.doi.org/10.3390/nu14102072DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9146862PMC
May 2022

Functional assessment of the cell-autonomous role of NADase CD38 in regulating CD8 T cell exhaustion.

iScience 2022 May 4;25(5):104347. Epub 2022 May 4.

CAMS Key Laboratory of Synthetic Biology Regulatory Element, Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.

Exhausted CD8 T cells with limited effector functions and high expression of multiple co-inhibitory receptors are one of the main barriers hindering antitumor immunity. The NADase CD38 has received considerable attention as a biomarker of CD8 T cell exhaustion, but it remains unclear whether the increased CD38 directly promotes T cell dysfunctionality. Here, we surprisingly found that although deficiency partially reverses NAD degradation and T cell dysfunction , the terminal exhausted differentiation of adoptively transferred CD8 T cells in tumor is not impacted by either deficiency or overexpression of CD38. Monitoring the dynamic NAD levels shows that NAD levels are comparable between tumor infiltrated WT and OT-1 cells. Therefore, our results suggest that decreased NAD are correlated with T cell dysfunction, but deficiency of CD38 is not enough for rescuing NAD in tumor infiltrated CD8 T cells and fails to increase the efficacy of antitumor T cell therapy.
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http://dx.doi.org/10.1016/j.isci.2022.104347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117873PMC
May 2022

Highly efficient removal of toluene over Cu-V oxides modified γ-AlO in the presence of SO.

J Hazard Mater 2022 Aug 2;436:129041. Epub 2022 May 2.

School of Energy and Power Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, Wuhan 430074, China.

Developing efficient catalysts with good resistance to complex flue gas is essential for VOCs removal in coal-fired flue gas. In this study, by exploring the effect of transition metal oxide additive, metal loading and bimetallic synergy on toluene oxidation in coal-fired flue gas, 10Cu-3V/γ-AlO is identified as the optimal catalyst. It achieves 90% of CO generation at 350 ℃, which is decreased by ca. 46 ℃ compared with 13Cu/γ-AlO. And it also exhibits good resistance to HO and good stability. ICP-OES, N adsorption-desorption isotherms, XRD, TEM, XPS, EPR and H-TPR analyses were applied to characterize the catalyst composition and physicochemical properties. Doping V into 13Cu/γ-AlO not only leads to better dispersity of CuO and homogeneous elements distribution that benefits to produce more active centers, but also constitutes the redox cycle of V + Cu ↔ V + Cu which induces more surface chemical oxygen (O). Moreover, since SO is the main inhibiting factor in toluene oxidation, the SO poisoning mechanism was illustrated by XPS, TG and in situ DRIFT analyses in depth.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129041DOI Listing
August 2022

Preparation of β-cyclodextrin/dopamine hydrochloride-graphene oxide and its adsorption properties for sulfonamide antibiotics.

Environ Sci Pollut Res Int 2022 May 18. Epub 2022 May 18.

School of Biology, Food, and Environment, Hefei University, No. 99 Jinxiu Road, Hefei, 230601, China.

To develop high-efficiency antibiotic adsorbents, β-cyclodextrin and dopamine hydrochloride were used to modify graphene oxide to prepare a new type of ternary composite material (β-cyclodextrin/dopamine hydrochloride-graphene oxide, CD-DGO). The material was characterized using scanning electron microscopy, Fourier infrared spectrometry, transmission electron microscopy, and specific surface area optical analysis. Two typical sulfonamides antibiotics (sulfamethoxazole, sulfadiazine) adsorption capacity were evaluated in terms of the dosage of composite materials, the ratio of each component, and the pH of the solution. We analyzed the adsorption characteristics via adsorption kinetics and adsorption isotherms, and then investigated the stability of the adsorbent through desorption and regeneration of the adsorbent. The results show that the adsorption effect of sulfonamides antibiotics is best at pH = 2; the adsorption kinetics conform to the pseudo-second-order kinetic model, and the adsorption equilibrium follows the Langmuir adsorption isotherm; the maximum adsorption capacity of CD-DGO for sulfamethoxazole and sulfadiazine is 144 mg·g and 152 mg·g, respectively. The material has good reusability, and the dominant force in the adsorption process is the π-π electron conjugation effect with hydrogen bonding. This offers a theoretical basis for the treatment of sulfonamides antibiotics water pollution.
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http://dx.doi.org/10.1007/s11356-022-20828-4DOI Listing
May 2022

Ferrocene-modified Uio-66-NH hybrids with g-CN as enhanced photocatalysts for degradation of bisphenol A under visible light.

J Hazard Mater 2022 Aug 4;436:129052. Epub 2022 May 4.

State Key Laboratory of Chemical Engineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, PR China.

Designing graphitic carbon nitride (CN) based heterostructured photocatalysts with high catalytic activity is highly desired for peroxymonosulfate (PMS) activation to degrade organic pollutants from water. Herein, a novel heterostructured composite ([email protected]) consisting of ferrocene-modified Uio-66-NH (U-F) and CN was synthesized. The [email protected] exhibited superior photocatalytic performance to degrade bisphenol A (BPA) in the presence of PMS under visible light. The experimental results indicated that BPA could be removed entirely by [email protected] within 60 min under visible light irradiation. In addition, the outstanding photocatalytic activity could be maintained at high level in a wide pH range, appropriate temperature region and natural water condition. Benefiting from the good chemical stability, outstanding optical property and in-situ generation of interfacial heterojunction of [email protected], the interfacial transport of photogenerated charges could follow the Z-scheme mechanism, which can accelerate the charge separation and transport to yield abundant reactive active species (ROS) to efficiently active PMS and under visible light. This work provides a novel approach to design CN-based heterostructured photocatalysts with high stability and superior photocatalytic activity for environmental remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129052DOI Listing
August 2022

Ovulation induction is associated with altered growth but with preservation of normal metabolic function in murine offspring.

F S Sci 2021 08 10;2(3):259-267. Epub 2021 Jun 10.

University of California San Francisco, San Francisco, California. Electronic address:

Objective: To study the effects of ovulation induction on mouse postnatal health, with a focus on growth pattern and glucose tolerance. To study the effect of ovulation induction on DNA methylation, we took advantage of the agouti viable yellow (A) mouse.

Design: Animal study.

Setting: University Setting.

Animals: Agouti viable yellow (A) mice on a C57BL/6 background.

Intervention(s): A female mice were either allowed to mate spontaneously (control group, C) or after superovulation with 5 IU of PMSG and hCG (ovulation induction group, OI).

Main Outcome Measure(s): Birth parameters and postnatal growth of the offspring were followed up to 29 weeks of age. Body composition analysis was performed by EchoMRI; fasting insulin, intraperitoneal glucose tolerance tests, and glucose-stimulated insulin secretion by beta cells were assessed to study glucose metabolism.

Result(s): Mice born to superovulated dams had lower survival rates, shorter anogenital distances, and shorter crown-rump lengths. Female mice generated by OI weighed less at birth, whereas male mice generated by OI had lower weight gain and had reduced lean mass. Glucose parameters, including islet functions, did not differ between the groups. No difference in agouti coat color was noted between the groups.

Conclusion(s): Ovulation induction resulted in mice having increased morphometric differences at birth and male mice showing reduced weight gain but no difference in glucose tolerance or agouti coat color.
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http://dx.doi.org/10.1016/j.xfss.2021.06.002DOI Listing
August 2021

Persistent Hemodynamic Depression After Carotid Artery Stenting: A Review and Update.

Vasc Endovascular Surg 2022 May 13:15385744221096220. Epub 2022 May 13.

Department of Neurology, 560852Shenzhen Samii International Medical Center (The Fourth People's Hospital of Shenzhen), Shenzhen, China.

According to the American Heart Association, ischemic stroke is the second leading cause of death globally and is responsible for approximately 11% of deaths. Carotid endarterectomy (CEA) is the standard treatment for moderate or severe extracranial internal carotid artery (ICA) stenoses. With the development of materials and technology in neurointervention, the Centers for Medicare and Medicaid Services (CMS) have proposed that carotid artery stenting (CAS) can serve as an alternative treatment for CEA. As CAS is widely used worldwide, comorbidities, especially persistent hemodynamic depression (PHD) and stroke, have attracted public attention. In this review, we summarized the current advances in PHD after CAS. A better understanding of CAS-related PHD may inspire the design of potential prognostic and therapeutic tools.
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http://dx.doi.org/10.1177/15385744221096220DOI Listing
May 2022
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