Publications by authors named "Xiaowei Liu"

491 Publications

Removal of antibiotic-resistant genes during drinking water treatment: A review.

J Environ Sci (China) 2021 Jun 30;104:415-429. Epub 2020 Dec 30.

Zhejiang Key Laboratory of Drinking Water Safety and Distribution Technology, Zhejiang University, Hangzhou 310058, China; Ocean College, Zhejiang University, Hangzhou 310058, China. Electronic address:

Once contaminate the drinking water source, antibiotic resistance genes (ARGs) will propagate in drinking water systems and pose a serious risk to human health. Therefore, the drinking water treatment processes (DWTPs) are critical to manage the risks posed by ARGs. This study summarizes the prevalence of ARGs in raw water sources and treated drinking water worldwide. In addition, the removal efficiency of ARGs and related mechanisms by different DWTPs are reviewed. Abiotic and biotic factors that affect ARGs elimination are also discussed. The data on presence of ARGs in drinking water help come to the conclusion that ARGs pollution is prevalent and deserves a high priority. Generally, DWTPs indeed achieve ARGs removal, but some biological treatment processes such as biological activated carbon filtration may promote antibiotic resistance due to the enrichment of ARGs in the biofilm. The finding that disinfection and membrane filtration are superior to other DWTPs adds weight to the advice that DWTPs should adopt multiple disinfection barriers, as well as keep sufficient chlorine residuals to inhibit re-growth of ARGs during subsequent distribution. Mechanistically, DWTPs obtain direct and inderect ARGs reduction through DNA damage and interception of host bacterias of ARGs. Thus, escaping of intracellular ARGs to extracellular environment, induced by DWTPs, should be advoided. This review provides the theoretical support for developping efficient reduction technologies of ARGs. Future study should focus on ARGs controlling in terms of transmissibility or persistence through DWTPs due to their biological related nature and ubiquitous presence of biofilm in the treatment unit.
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http://dx.doi.org/10.1016/j.jes.2020.12.023DOI Listing
June 2021

Ultraeven Mo-Doped CoP Nanocrystals as Bifunctional Electrocatalyst for Efficient Overall Water Splitting.

Langmuir 2021 May 7. Epub 2021 May 7.

Laboratory for Low Dimensional Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.

To develop precious-metal-free bifunctional catalysts for overall water splitting, ultraeven Mo-doped CoP composites (Mo-CoP) have been fabricated by an in situ phosphorization protocol using CoMo-layered double hydroxide (CoMo-LDH) as the precursor. The ordered arrangement of cations in the CoMo-LDH could be easily phosphored and generate the ultraevenly dispersed Mo element within the CoP structure, resulting in the excellent bifunctional catalyst for overall water splitting. The hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) catalytic activities of the composites present an increase first and then a decreasing tendency with increased Mo doping content. Among all the Mo-doped CoP materials, the composite with a Mo/Co mole ratio of 1/2.3 presents the highest HER activity and stability in acidic conditions. At the current density of -10 mA·cm in 0.5 M HSO, the overpotential is only 116 mV. In addition, the composite also presents excellent HER and OER performance under alkaline conditions. The overpotential is 118 mV for HER and 317 mV for OER at 10 mA cm in 1 M KOH. It requires a cell voltage of 1.7 V to achieve a current density of 10 mA·cm and maintains a stable water-splitting current for at least 24 h, which is superior to most reported alkaline media. This simple and efficient synthetic approach could also be used for ultraeven doping between other transition metal ions.
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http://dx.doi.org/10.1021/acs.langmuir.1c00524DOI Listing
May 2021

Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway.

Front Pharmacol 2021 12;12:639574. Epub 2021 Apr 12.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.

Idiopathic pulmonary fibrosis is a progressive lung disease with high mortality and limited therapy that is characterized by epithelial cell damage and fibroblast activation. Ellagic acid is a natural polyphenol compound widely found in fruits and nuts that has multiple pharmacological activities. In this study, we explored the potential effects and mechanisms of Ellagic acid on pulmonary fibrosis and . studies showed that Ellagic acid significantly alleviated bleomycin (BLM)-induced pulmonary fibrosis in mice. experiments indicated that Ellagic acid could suppress Wnt signaling and attenuate Wnt3a-induced myofibroblast activation and the phosphorylation of Erk2 and Akt. Further studies showed that Ellagic acid could induce autophagy formation in myofibroblasts mainly by suppressing mTOR signaling and promoting apoptosis of myofibroblasts. experiments revealed that Ellagic acid significantly inhibited myofibroblast activation and promoted autophagy formation. Taken together, our results show that Ellagic acid effectively attenuates BLM-induced pulmonary fibrosis in mice by suppressing myofibroblast activation and promoting autophagy and apoptosis of myofibroblasts by inhibiting the Wnt signaling pathway.
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http://dx.doi.org/10.3389/fphar.2021.639574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072668PMC
April 2021

Association Analysis of Polymorphisms in and with Mild Cognitive Impairment in Elderly Carrying Allele.

Neuropsychiatr Dis Treat 2021 21;17:1125-1133. Epub 2021 Apr 21.

Department of Geriatric Psychiatry, The Affiliated Wuxi Mental Health Center with Nanjing Medical University, Wuxi, Jiangsu Province, People's Republic of China.

Background: Apolipoprotein () is recognized as an independent risk factor for mild cognitive impairment (MCI). However, not everyone with the ε4 allele develops MCI, suggesting that other susceptibility genes exist. This study aimed to identify MCI susceptibility genes, including , and , in elderly Han Chinese and to verify their association with ε4 allele in MCI onset.

Methods: To determine whether polymorphisms in (rs6733839, rs7561528), (rs2228479), (rs10164112), and (rs6859) occurred in elderly MCI patients carrying allele, we carried out a case-control study including 285 MCI patients and 326 healthy controls.

Results: Statistically significant differences in the proportion of carriers, and BESCI, ADAS-cog, and CNT scores existed between the NC and MCI groups (all < 0.01). Frequencies of the rs10164112 T and rs6859 A alleles were significantly higher in the latter than in the former ( = 0.01; 0.029). However, no significant differences in allele and genotype distribution of (rs6733839, rs7561528) and (rs2228479) existed between samples in our two groups (all > 0.05). When stratified by status (carriers/non-carriers), genotype frequencies of rs7561528, rs10164112, and rs6859 among the four groups (NCε4+, NCε4-, MCIε4+, MCIε4-) were significantly different. Additionally, our results suggest a significant association between MCI and rs7561528, rs10164112, and rs6859 (all <0.05) in elderly carriers.

Conclusion: This suggests that among the Han Chinese, MCI in elderly carriers may be related to the (rs7561528), (rs10164112) and (rs6859). Genotype AA of rs7561528 and TT of rs10164112 might be protective factors against MCI in elderly carriers.
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http://dx.doi.org/10.2147/NDT.S296144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071212PMC
April 2021

Transfer hydrogenation of CO into formaldehyde from aqueous glycerol heterogeneously catalyzed by Ru bound to LDH.

Chem Commun (Camb) 2021 Apr 27. Epub 2021 Apr 27.

State Key Laboratory of Coal Combustion, Huazhong University of Science and Technology, Wuhan 430074, China.

Aqueous glycerol was used in this study as a liquid-phase hydrogen source for the hydrogenation of CO2. It was found that hydrogen could be efficiently evolved from aqueous glycerol upon highly dispersed Ru on layered double hydroxide (LDH), inducing the transformation of CO2 into formaldehyde under base-free conditions at low temperature.
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http://dx.doi.org/10.1039/d1cc01299aDOI Listing
April 2021

Turbidity prediction of lake-type raw water using random forest model based on meteorological data: A case study of Tai lake, China.

J Environ Manage 2021 Apr 20;290:112657. Epub 2021 Apr 20.

Zhejiang Key Laboratory of Drinking Water Safety and Distribution Technology, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Turbidity is an indication of water quality and enables the growth of pathogenic microorganisms. For drinking water treatment plants (DWTPs), violent fluctuations in turbidity are highly disruptive to operational performance due to the lag in process parameter adjustments. Such risks must be carefully managed to guarantee safe drinking water. Machine learning techniques have been proven to be effective for modeling complex nonlinear environmental systems, and this study adopted such a technique to develop a model for predicting source water turbidity for DWTPs to allow DWTPs to make proactive interventions in advance. A random forest (RF) model used preprocessed (empirical mode decomposition and quartile rejecting) meteorological factors (wind speed, wind direction, air temperature, and rainfall) as the input variables, to establish the turbidity prediction of a lake with significant turbidity in China's South Tai Lake. The modeling process included four main stages: (1) source data analysis, (2) raw data preprocessing, (3) modeling and tuning, and (4) model evaluation. The results of the RF model indicated that the correlation coefficient between the predicted and actual sequences is over 0.7, and more than 55% of the predicted values could control the errors within 20% compared to the actual measured values, suggesting that machine learning techniques are suitable for predicting the turbidity of raw source water. It was found that the RF model can provide a modest performance boost because of its stronger capacity to capture nonlinear interactions in the data. The findings of this study can inform the development of turbidity prediction models using readily available meteorological forecast data. The model can be applied to other DWTPs using similar shallow lakes as water sources.
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http://dx.doi.org/10.1016/j.jenvman.2021.112657DOI Listing
April 2021

The complete mitogenome of freshwater gammarid (Crustacea: Amphipoda).

Mitochondrial DNA B Resour 2021 Apr 8;6(4):1369-1371. Epub 2021 Apr 8.

Key Laboratory of Fisheries Resources in Inland Water of Jiangsu Province, Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, PR China.

The freshwater gammarid is an important composition of benthic community. In this study, the complete mitogenome sequences of are determined using next-generation and PacBio long-read sequencing. The mitogenome of is 15,099 bp in size, and consisted of 13 protein-coding genes, two ribosomal genes, 22 genes, and a putative control region. Gene arrangement is as same as that of . The base composition of the entire mitogenome showed a conspicuous A + T bias of 69.4%. The mitogenome data produced in this study provides a useful resource for future evolutionary and ecological studies.
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http://dx.doi.org/10.1080/23802359.2021.1910079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8043560PMC
April 2021

A phase 1 study of prexasertib (LY2606368), a CHK1/2 inhibitor, in pediatric patients with recurrent or refractory solid tumors, including CNS tumors: A report from the Children's Oncology Group Pediatric Early Phase Clinical Trials Network (ADVL1515).

Pediatr Blood Cancer 2021 Apr 21:e29065. Epub 2021 Apr 21.

Center for Cancer and Blood Disorders, Phoenix Children's Hospital, Institute for Molecular Medicine, Department of Child Health, University of Arizona College of Medicine-Phoenix, Phoenix, Arizona.

Background: Prexasertib (LY2606368) is a novel, second-generation, selective dual inhibitor of checkpoint kinase proteins 1 (CHK1) and 2 (CHK2). We conducted a phase 1 trial of prexasertib to estimate the maximum-tolerated dose (MTD) and/or recommended phase 2 dose (RP2D), to define and describe the toxicities, and to characterize the pharmacokinetics (PK) of prexasertib in pediatric patients with recurrent or refractory solid and central nervous system (CNS) tumors.

Methods: Prexasertib was administered intravenously (i.v.) on days 1 and 15 of a 28-day cycle. Four dose levels, 80, 100, 125, and 150 mg/m , were evaluated using a rolling-six design. PK analysis was performed during cycle 1. Tumor tissue was examined for biomarkers (CHK1 and TP53) of prexasertib activity.

Results: Thirty patients were enrolled; 25 were evaluable. The median age was 9.5 years (range: 2-20) and 21 (70%) were male. Twelve patients (40%) had solid tumors and 18 patients (60%) had CNS tumors. There were no cycle 1 or later dose-limiting toxicities. Common cycle 1, drug-related grade 3/4 toxicities (> 10% of patients) included neutropenia (100%), leukopenia (68%), thrombocytopenia (24%), lymphopenia (24%), and anemia (12%). There were no objective responses; best overall response was stable disease in three patients for five cycles (hepatocellular carcinoma), three cycles (ependymoma), and five cycles (undifferentiated sarcoma). The PK appeared dose proportional across the 80-150 mg/m dose range.

Conclusions: Although the MTD of prexasertib was not defined by this study, 150 mg/m administered i.v. on days 1 and 15 of a 28-day cycle was determined to be the RP2D.
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http://dx.doi.org/10.1002/pbc.29065DOI Listing
April 2021

Intestinal microbiota and antibiotic-associated acute gastrointestinal injury in sepsis mice.

Aging (Albany NY) 2021 Mar 26;13(7):10099-10111. Epub 2021 Mar 26.

Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, Heilongjiang, China.

Background: To investigate the changes of intestinal microbiota and metabolites in sepsis mice with acute gastrointestinal injury before and after the use of antibiotics, and to explore the possible effects of these changes on the body.

Methods: Twenty-four 6-8-w-old SPF-grade C57BL/6J male mice were selected, and the mice were randomly divided into three groups. The mice were treated by tail vein injection for 3 days. The intestinal motility of mice after administration was detected. The mice feces were collected for 16S rRNA and Untargeted metabonomics detection.

Results: The use of antibiotics in sepsis mice can change the composition of intestinal microbiota and metabolites. LD3, AD3 and LAD3 samples had significant differences in bacterial species. was the species with a significant difference in LAD3. In addition, we found that the composition of those intestinal microbiota were correlated with changes in intestinal motility. The untargeted metabolomics analysis showed that the fecal metabolites of LD3 and LAD3 samples were significantly different. In addition to the basic metabolites, Benzoic acid and 4-Hydroxybenzoic acid were also found, and was associated with them.

Conclusions: The use of antibiotics in sepsis mice can lead to changes in the intestinal microbiota and metabolite levels, which may be related to the severity of acute gastrointestinal injury in sepsis mice. Inhibiting in the intestine and using Benzoic acid and 4-Hydroxybenzoic acid as a marker for the production of may reduce the inflammatory degree of acute gastrointestinal injury in sepsis.
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http://dx.doi.org/10.18632/aging.202768DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064167PMC
March 2021

Association study of polymorphisms of endoplasmic reticulum aminopeptidase 1 gene with preeclampsia in Chinese populations.

Clin Exp Hypertens 2021 Apr 2:1-5. Epub 2021 Apr 2.

Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Objective: To explore the correlation of polymorphisms of endoplasmic reticulum aminopeptidase-1 (ERAP-1) gene in Chinese women with preeclampsia.

Method: In this case-control study, 51 severely preeclamptic and 48 healthy pregnant women were retrospectively involved in Beijing Obstetrics and Gynecology Hospital from October 2017 to October 2019. The venous blood and umbilical cord blood were collected to analyze different single nucleotide polymorphisms (SNPs) in ERAP-1 gene in both groups.

Result: We firstly screened nine SNPs that were significantly different between two groups (< .1) via univariate analysis. Pearson Chi-Square Test and Fisher's Exact Test were performed to verify the correlation of SNPs with preeclampsia. Finally, genotype TC located in 96121524 position was associated with an increased risk of preeclampsia (= .020, OR = 2.002, 95% CI: 0.687-5.831).

Conclusion: There might be a correlation between polymorphism of 96121524 in ERAP-1 gene and preeclampsia susceptibility in pregnant women of Han nationality in Beijing.
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http://dx.doi.org/10.1080/10641963.2021.1907400DOI Listing
April 2021

Noncarrier embryo selection and transfer in preimplantation genetic testing cycles for reciprocal translocation by Oxford Nanopore Technologies.

J Genet Genomics 2020 11 13;47(11):718-721. Epub 2020 May 13.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, 250012, China; Key laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, 250012, China.

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http://dx.doi.org/10.1016/j.jgg.2020.05.001DOI Listing
November 2020

UV-activated permanganate process for micro-organic pollutant degradation: efficiency, mechanism and influencing factors.

Water Sci Technol 2021 Mar;83(6):1278-1285

Zhejiang Key Laboratory of Drinking Water Safety and Distribution Technology, Zhejiang University, Hangzhou 310058, China E-mail:

Ultraviolet-activated permanganate (UV/PM) process is a novel advanced oxidation process (AOP), but its application potential remains to be evaluated. This work investigates the degradation of refractory organic pollutant by UV/PM in terms of efficiency, mechanism, and influencing factors. The target compound benzoic acid (BA), which is a micro-organic pollutant and is resistant to PM and UV treatment, can be efficiently degraded by UV/PM. The electron paramagnetic resonance spectra directly supported the formation of hydroxyl radical (HO•) and superoxide radical (O) from UV photolysis of PM. Competitive kinetics experiments verified that O acted as precursor of HO• and the good degradation performance of BA was due to the involvement of HO• and manganese(V). The rate constants of BA degradation showed a positive linear relationship with PM dosage in the range of 0.5-20 mg·L, and the degradation process was significantly influenced by solution pH and natural organic matters but insensitive to chloride and bicarbonate at environmentally relevant concentrations. Compared to the typical UV-based AOP UV/hydrogen peroxide, UV/PM is a little inferior, indicating that optimization and enhancement is needed for this process before its possible practical application.
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http://dx.doi.org/10.2166/wst.2021.068DOI Listing
March 2021

Redox-sensitive carrier-free nanoparticles self-assembled by disulfide-linked paclitaxel-tetramethylpyrazine conjugate for combination cancer chemotherapy.

Theranostics 2021 20;11(9):4171-4186. Epub 2021 Feb 20.

College of Materials Science and Engineering, Nanjing Tech Universit`y, Nanjing 211816, People's Republic of China.

Combinations of two or more therapeutic agents targeting different signaling pathways involved in tumor progression can have synergistic anticancer effects. However, combination chemotherapies are greatly limited by the different pharmacokinetics, tumor targeting, and cellular uptake capacities of the combined drugs. We have previously demonstrated the potential synergistic efficacy of paclitaxel (PTX) and the natural anti-angiogenic agent tetramethylpyrazine (TMP) for suppressing ovarian carcinoma growth. An efficient, facile, and smart nanosystem to deliver PTX and TMP simultaneously is greatly desired. We constructed a redox-sensitive nanosystem based on the amphiphilic PTX-ss-TMP conjugate, in which PTX and TMP are linked by a disulfide bond. We characterized the structure of the drug conjugate by H NMR and LC-MS, and then prepared PTX-ss-TMP NPs by a one-step nanoprecipitation method. We investigated the redox sensitivity, tumor-targeting ability, anticancer efficacy, and anti-angiogenesis activity of PTX-ss-TMP NPs and . The amphiphilic PTX-ss-TMP conjugate readily self-assembled into stable nanoparticles in aqueous solution with a low critical association concentration of 1.35 µg/mL, well-defined spherical structure, small particle size (152 nm), high drug loading, redox-responsive drug release, high biocompatibility, and high storage stability. In cancer cells pretreated with GSH-OEt, PTX-ss-TMP NPs exhibited higher cytotoxicity, apoptosis rate, and cell-cycle arrest than monotherapy or combination therapy with free drugs, which was attributed to their improved cellular uptake and rapid intracellular drug release. Additionally, PTX-ss-TMP NPs also had a stronger anti-angiogenesis effect in HUVECs than free drug, which was mediated by VEGFR2-involved downstream signals. Finally, PTX-ss-TMP NPs showed tumor-specific accumulation and excellent antitumor activity in A2780 xenograft mice compared with free drug. These and results provide clear evidence that this redox-responsive carrier-free nanosystem with intrinsic amphiphilicity has great potential for combination cancer chemotherapy.
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http://dx.doi.org/10.7150/thno.42260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7977472PMC
February 2021

Unexplained ascites caused by myeloid sarcoma with omentum involvement.

Rev Esp Enferm Dig 2021 Mar 18. Epub 2021 Mar 18.

Gastroenterology, Xiangya Hospital Central South University, China.

A previously healthy 40-year-old male was admitted with a 10-day history of abdominal distension. Physical examinations revealed bulging flanks with a fluid wave and positive shifting dullness signs.
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http://dx.doi.org/10.17235/reed.2021.7892/2021DOI Listing
March 2021

A 36-week multicenter, randomized, double-blind, placebo-controlled, parallel-group, phase 3 clinical trial of sodium oligomannate for mild-to-moderate Alzheimer's dementia.

Alzheimers Res Ther 2021 03 17;13(1):62. Epub 2021 Mar 17.

Department of Geriatric psychiatry, Wuxi Mental Health Center, Wuxi, China.

Background: New therapies are urgently needed for Alzheimer's disease (AD). Sodium oligomannate (GV-971) is a marine-derived oligosaccharide with a novel proposed mechanism of action. The first phase 3 clinical trial of GV-971 has been completed in China.

Methods: We conducted a phase 3, double-blind, placebo-controlled trial in participants with mild-to-moderate AD to assess GV-971 efficacy and safety. Participants were randomized to placebo or GV-971 (900 mg) for 36 weeks. The primary outcome was the drug-placebo difference in change from baseline on the 12-item cognitive subscale of the Alzheimer's Disease Assessment Scale (ADAS-cog12). Secondary endpoints were drug-placebo differences on the Clinician's Interview-Based Impression of Change with caregiver input (CIBIC+), Alzheimer's Disease Cooperative Study-Activities of Daily Living (ADCS-ADL) scale, and Neuropsychiatric Inventory (NPI). Safety and tolerability were monitored.

Results: A total of 818 participants were randomized: 408 to GV-971 and 410 to placebo. A significant drug-placebo difference on the ADAS-Cog12 favoring GV-971 was present at each measurement time point, measurable at the week 4 visit and continuing throughout the trial. The difference between the groups in change from baseline was - 2.15 points (95% confidence interval, - 3.07 to - 1.23; p < 0.0001; effect size 0.531) after 36 weeks of treatment. Treatment-emergent adverse event incidence was comparable between active treatment and placebo (73.9%, 75.4%). Two deaths determined to be unrelated to drug effects occurred in the GV-971 group.

Conclusions: GV-971 demonstrated significant efficacy in improving cognition with sustained improvement across all observation periods of a 36-week trial. GV-971 was safe and well-tolerated.

Trial Registration: ClinicalTrials.gov, NCT0229391 5. Registered on November 19, 2014.
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http://dx.doi.org/10.1186/s13195-021-00795-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967962PMC
March 2021

Polymer-Water Interaction Enabled Intelligent Moisture Regulation in Hydrogels.

J Phys Chem Lett 2021 Mar 9;12(10):2587-2592. Epub 2021 Mar 9.

MOE Key Laboratory of Hydrodynamic Transients, School of Power and Mechanical Engineering, Wuhan University, Wuhan, Hubei 430072, P. R. China.

The water-vapor transition is critical for hydrogels in a collection of applications. However, how the polymer-water interaction along with the nature of the structure affect the macroscopic water-vapor transition remains a challenging question to answer. In this work, we tested the moisture transfer behaviors of a series of hydrogels at different humidities and found some hydrogels capable of lowering their surface vapor pressure to stop dehydration at low humidity and absorbing water from ambient air to recover toward initial states at high humidity. Through molecular dynamic simulations, we demonstrate that water inside these hydrogels undergoes increasing intensive intermolecular bonding during evaporation. The increased intermolecular bonding reduces the vapor pressure of the hydrogels and leads to the self-regulation. More interestingly, we demonstrate the self-regulation is closely related to the Young's modulus of hydrogels. These results provide further insight into the mechanism of the water-vapor transition in hydrogels and show potential in a broad range of future applications.
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http://dx.doi.org/10.1021/acs.jpclett.1c00034DOI Listing
March 2021

R-JaunLab: Automatic Multi-Class Recognition of Jaundice on Photos of Subjects with Region Annotation Networks.

J Digit Imaging 2021 Feb 25. Epub 2021 Feb 25.

Department of Gastroenterology and Hepatology, Shenzhen University General Hospital, Shenzhen, 518055, China.

Jaundice occurs as a symptom of various diseases, such as hepatitis, the liver cancer, gallbladder or pancreas. Therefore, clinical measurement with special equipment is a common method that is used to identify the total serum bilirubin level in patients. Fully automated multi-class recognition of jaundice combines two key issues: (1) the critical difficulties in multi-class recognition of jaundice approaches contrasting with the binary class and (2) the subtle difficulties in multi-class recognition of jaundice represent extensive individuals variability of high-resolution photos of subjects, huge coherency between healthy controls and occult jaundice, as well as broadly inhomogeneous color distribution. We introduce a novel approach for multi-class recognition of jaundice to detect occult jaundice, obvious jaundice and healthy controls. First, region annotation network is developed and trained to propose eye candidates. Subsequently, an efficient jaundice recognizer is proposed to learn similarities, context, localization features and globalization characteristics on photos of subjects. Finally, both networks are unified by using shared convolutional layer. Evaluation of the structured model in a comparative study resulted in a significant performance boost (categorical accuracy for mean 91.38%) over the independent human observer. Our work was exceeded against the state-of-the-art convolutional neural network (96.85% and 90.06% for training and validation subset, respectively) and showed a remarkable categorical result for mean 95.33% on testing subset. The proposed network makes a performance better than physicians. This work demonstrates the strength of our proposal to help bringing an efficient tool for multi-class recognition of jaundice into clinical practice.
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http://dx.doi.org/10.1007/s10278-021-00432-7DOI Listing
February 2021

Combination of MAPK inhibition with photothermal therapy synergistically augments the anti-tumor efficacy of immune checkpoint blockade.

J Control Release 2021 Apr 22;332:194-209. Epub 2021 Feb 22.

Laboratory of Tumor Targeted and Immune Therapy, Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, 610041 Chengdu, Sichuan, China. Electronic address:

The combination of MAPK-targeted therapy and immune checkpoint blockade is one of the most promising regimens for patients with advanced melanoma. However, the synergistic efficacy of the combo regimen is still controversial in clinical trials. Here, we report that MAPK inhibition induced T-cell suppression within tumor microenvironment is mediated by attenuation of HSP27/HSP70 and deficiency of neoantigen presentation. To address this problem, we designed a photothermal-responsive on-demand controlled drug release gold nano-system to carry BRAF inhibitor. The nano-system can be specifically delivered into tumor cells rather than T-cells, and effectively transformed the optical energy into heat energy upon laser irradiation. Combination of photothermal and targeted therapy significantly promoted immunogenic cell death and T-cell infiltration. On top of this regimen, systematically administration of PD-1 antibody not only suppressed local-treated tumor but also inhibited abscopal tumor by enhancing generalized immune-related antitumor response. More importantly, the triple-combo regimen could efficiently convert immune "cold" tumors into "hot" ones. In conclusion, our research proves the advantage of photothermal-targeted-immune triple combinatorial regimen in treating tumors which are clinical unresectable multifocal and lack of T-cell infiltration.
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http://dx.doi.org/10.1016/j.jconrel.2021.02.020DOI Listing
April 2021

Predictive values of various serum biomarkers in women with suspected preeclampsia: A prospective study.

J Clin Lab Anal 2021 Feb 22:e23740. Epub 2021 Feb 22.

Department of Laboratory Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Background: Preeclampsia (PE) prediction has been shown to improve the maternal and fetal outcomes in pregnancy. We aimed to evaluate the PE prediction values of a series of serum biomarkers.

Methods: The singleton pregnant women (20-36 gestational weeks) with PE-related clinical and/or laboratory presentations were recruited and had the blood drawn at their first visits. The following markers were tested with the collected serum samples: soluble fms-like tyrosine kinase 1 (sFlt-1), placental growth factor (PlGF), thrombomodulin (TM), tissue plasminogen activator inhibitor complex (tPAI-C), complement factors C1q, B, H, glycosylated fibronectin (GlyFn), pregnancy-associated plasma protein-A2 (PAPP-A2), blood urea nitrogen (BUN), creatinine (Cre), uric acid (UA), and cystatin C (Cysc).

Results: Of the 196 recruited subjects, 25% (n = 49) developed preeclampsia before delivery, and 75% remained preeclampsia negative (n = 147). The serum levels of sFlt-1, BUN, Cre, UA, Cysc, and PAPP-A2 were significantly elevated, and the PlGF level was significantly decreased in the preeclampsia-positive patients. In the receiver operating characteristics (ROC) analyses, the area under the curves were listed in the order of decreasing values: 0.73 (UA), 0.67 (sFlt-1/PlGF), 0.66 (Cysc), 0.65 (GlyFn/PlGF), 0.64 (PAPP-A2/PlGF), 0.63 (BUN), 0.63 (Cre), and 0.60 (PAPP-A2). The positive predictive values of these serum markers were between 33.1% and 58.5%, and the negative predictive values were between 80.9% and 89.5%.

Conclusions: The serum markers investigated in current study showed better performance in ruling out than ruling in PE. Absence of pre-defined latency period between blood draw and the onset of PE limits the clinical utility of these markers.
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http://dx.doi.org/10.1002/jcla.23740DOI Listing
February 2021

A Pyrene Fluorescent Probe for Rapid Detection of Ferric Ions.

J Fluoresc 2021 May 20;31(3):713-718. Epub 2021 Feb 20.

Shandong Taihe Water Treatment Technologies Co., Ltd., Zaozhuang, Shandong, China.

The 1,3,6,8-pyrenetetrasulfonic acid tetrasodium salt (PTSA) is a pyrene derivative with high fluorescence characteristics and is widely used in fluorescence tracer. This study aims at investigating a simple and fast fluorescence detection method for determining the concentration of ferric ion by using PTSA, which the principle is that the fluorescence quenching of PTSA by ferric ions. Theoretical and experimental methods were adopted to deeply analyze its detection performance and characteristics. The fluorescence quenching phenomena under different pH conditions and the effect of the different interfering metal ions on PTSA/Fe system was studied. The results showed that the PTSA was quite promising for the fluorescence detection of trace ferric ions, and the limit of detection is 9 μg/L. This study is envisioned to provide inspirational insights on trace detection of iron ions, opening new routes for water monitoring use fluorescence properties.
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http://dx.doi.org/10.1007/s10895-021-02695-3DOI Listing
May 2021

Loganin Attenuates Septic Acute Renal Injury with the Participation of AKT and Nrf2/HO-1 Signaling Pathways.

Drug Des Devel Ther 2021 11;15:501-513. Epub 2021 Feb 11.

Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin, Heilongjiang 150001, People's Republic of China.

Purpose: Sepsis, a destructive inflammatory response syndrome, is the principal reason to induce death in the intensive care unit. Loganin has been proved to possess the property of anti-inflammation, antioxidant, neuroprotection, and sedation. The primary aim of this study was to evaluate whether Loganin could alleviate acute kidney injury (AKI) during sepsis and investigate the latent mechanisms.

Methods: Septic AKI models were established by cecal ligation and puncture (CLP) surgery in mice and given Loganin (20, 40, 80 mg/kg) by gavage. Lipopolysaccharides (LPS)-stimulated human kidney proximal tubular (HK2) cells incubated in Loganin (5, 10, 20 μ M) were used to explore the accurate mechanisms. Survival rate, renal function (creatinine and blood urea nitrogen), and renal pathological changes were detected in septic mice. Oxidative stress markers (SOD, GSH-Px, MDA, and SOD), mitochondrial membrane potential, mitochondrial calcium overload, and nuclear factor E2-related factor 2 (Nrf2)/heme-oxygenase 1 (HO-1) pathway activation in vivo and in vitro were determined by commercial kits and Western blot. Cell apoptosis, apoptotic-related protein (cleaved caspase-3, Bcl-2, and Bax) expression and protein kinase B (AKT) phosphorylation in vivo and in vitro were measured by TUNEL staining and Western blot. Finally, AKT blockage by 10 μM LY294002 or Nrf2 inhibition by10 μ M ML385 were utilized to prove the involvement of AKT and Nrf2/HO-1 pathway in AKI during sepsis.

Results: We found Loganin treatment (20, 40, 80 mg/kg) mitigated septic AKI reflected by elevated renal function and palliative pathological changes. Oxidative stress and apoptosis in the kidney and LPS-treated HK2 cells were also inhibited by Loganin administration, which was accompanied by AKT and Nrf2/HO-1 pathway activation. Besides, the protective effects of Loganin could be diminished by AKT or Nrf2 blockage, indicating the involvement of AKT and Nrf2/HO-1 pathway.

Conclusion: The results suggested that the protective effects of Loganin on AKI during sepsis might be mediated by AKT and Nrf2/HO-1 pathway signaling activation in kidney proximal tubular cells.
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http://dx.doi.org/10.2147/DDDT.S294266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886113PMC
February 2021

Obstetric outcomes for twins from different conception methods - A multicenter cross-sectional study from China.

Acta Obstet Gynecol Scand 2021 Feb 8. Epub 2021 Feb 8.

Department of Obstetrics and Gynecology, Harbin Nangang District Maternity Hospital, Nangang, Harbin Province, China.

Introduction: The effects of assisted reproductive technology on the outcomes of twin pregnancies are controversial. Therefore, the purpose of this study was to compare the maternal and perinatal outcomes of twin pregnancies conceived spontaneously and those conceived by assisted reproductive technology.

Material And Methods: This was a cross-sectional study performed at Peking Union Medical College Hospital (PUMCH). Data on twin pregnancies (conceived spontaneously and by in vitro fertilization [IVF]/intracytoplasmic sperm injection [ICSI]) were obtained from the National Birth Registry of China for the period between 1 October 2016, and 30 September 2017. The primary obstetric outcomes were compared between twin pregnancies conceived by different methods. Logistic regression analysis with 95% confidence intervals (95% CI) was used for the multivariate analysis.

Results: A total of 3270 twin pregnancies (2003 and 1209 conceived spontaneously and by IVF/ICSI, respectively) were identified. The proportion of twin pregnancies among all pregnancies was 3.4% (3332/97 278). Multiple regression analysis indicated that the incidences of gestational diabetes mellitus (adjusted odds ratio [AOR] = 1.42, 95% CI 1.10-1.83, p = 0.007), preterm premature rupture of membranes (AOR = 1.65, 95% CI 1.21-2.25, p = 0.002), placenta accreta spectrum (AOR = 2.12, 95% CI 1.42-3.17, p < 0.001) and postpartum hemorrhage (AOR = 1.38, 95% CI 1.02-1.86, p = 0.037) were significantly higher in the IVF/ICSI group than in the natural pregnancy group. Multivariate analysis also revealed that conception mode was not an independent risk factor for neonate outcomes.

Conclusions: In twin pregnancies, IVF/ICSI is independently associated with multiple maternal complications, including gestational diabetes mellitus, preterm premature rupture of membranes and placenta accreta spectrum compared with spontaneous conception, although potential residual confounders due to indications for assisted reproductive technology exist.
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http://dx.doi.org/10.1111/aogs.14116DOI Listing
February 2021

Genetics and Clinical Features of Noncompaction Cardiomyopathy in the Fetal Population.

Front Cardiovasc Med 2020 20;7:617561. Epub 2021 Jan 20.

Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Noncompaction Cardiomyopathy (NCCM) has been classified as primary genetic cardiomyopathy and has gained increasing clinical awareness; however, little is known about NCCM in the fetal population. We aimed to investigate the clinical characteristics and genetic spectrum of a fetal population with NCCM. We retrospectively reviewed all fetuses with a prenatal diagnosis of NCCM at a single center between October 2010 and December 2019. These cases were investigated for gestational age at diagnosis, gender, left or biventricular involvement, associated cardiac phenotypes, outcomes, and genetic testing data. We identified 37 fetuses with NCCM out of 49,898 fetuses, indicating that the incidence of NCCM in the fetal population was 0.07%. Of the 37 fetuses, 26 were male, ten were female and one was of unknown gender. NCCM involvement biventricle is the most common ( = 16, 43%), followed by confined to the left ventricle ( = 14, 38%). Nineteen (51%) had additional congenital heart defects, with right-sided lesions being the most common ( = 14, 74%), followed by ventricular septal defects ( = 10, 53%). Hydrops fetalis was present in 12 cases (32%), of which four were atypical (pericardial effusion only). Sequencing analysis was performed at autopsy ( = 19) or postnatally ( = 1) on 20 fetuses. Of the 20 fetuses undergoing copy number variation sequencing and whole-exome sequencing, nine (47%) had positive genetic results, including one with a pathogenic copy number variant and eight with pathogenic/likely pathogenic variants. Non-sarcomere gene mutations accounted for the vast majority ( = 7). In contrast, sarcomere gene mutations occurred in only one case (TPM1), and no mutations were identified in the three most common sarcomere genes (MYH7, TTN, and MYBPC3) of pediatric and adult patients. Pathogenic/likely pathogenic variants were significantly more frequent in fetuses with congenital heart defects than those without congenital heart defects. Our data demonstrate that fetal NCCM is a unique entity. Compared with pediatric and adult NCCM, fetal NCCM is more prone to biventricle involvement, more likely to be complicated with congenital heart defects, and has a distinct genetic spectrum.
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http://dx.doi.org/10.3389/fcvm.2020.617561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854697PMC
January 2021

Glyphosate, aminomethylphosphonic acid, and glufosinate ammonium in agricultural groundwater and surface water in China from 2017 to 2018: Occurrence, main drivers, and environmental risk assessment.

Sci Total Environ 2021 May 25;769:144396. Epub 2020 Dec 25.

Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin, China; Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, China; National Reference Laboratory for Agricultural Testing, China.

Glyphosate and glufosinate ammonium are the main herbicides used to control weeds in no-tillage agricultural fields in China. However, their leaching risk to groundwater and ecological risk to aquatic organisms remain unclear. From the agricultural basins among 10 provinces of China, glyphosate, its main metabolite aminomethylphosphonic acid (AMPA), and glufosinate ammonium were detected in 1.01%, 0.86%, 0% of 694 groundwater samples with the maximum concentrations of 2.09, 5.13, and <0.05 μg/L, and were detected in 14.3%, 15.8%, and 2.6% of 196 surface water samples with the maximum levels of 32.49, 10.31 and 13.15 μg/L. Furthermore, to evaluate the main drivers of exposure to the targets in water bodies, the fate models were used. The model simulation indicated that spray drift and overflow runoff were the key factors affecting the exposure to targets in surface water adjacent to rice field, whereas the spray drift deposition, runoff, and erosion induced the exposure to the targets in pond water close to dry land crop fields under different meteorological conditions and soil characteristics. The targets in groundwater posed a low risk to water consumption, while fish embryos might be at unacceptable risk due to glufosinate ammonium exposure in surface water with median risk quotient (RQ) equal to 55.6. The results highlight the spatial and seasonal distribution of glyphosate, AMPA, and glufosinate ammonium in groundwater and surface water in agricultural basins of China, providing the first evidence to the environmental risk of the targets to drinking water consumption and aquatic organism safety in China agriculture system.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144396DOI Listing
May 2021

Ultrasensitive ratiometric detection of Pb using DNA tetrahedron-mediated hyperbranched hybridization chain reaction.

Anal Chim Acta 2021 Feb 29;1147:170-177. Epub 2020 Dec 29.

Tianjin Key Laboratory of Biosensing and Molecular Recognition, Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin, 300071, PR China.

A fluorescent sensing strategy was developed for rapid, highly sensitive and specific detection of lead (II) ion (Pb) on the basis of Pb DNAzyme-controlled tetrahedral DNA nanostructure (TDN)-mediated hyper-branched hybridization chain reaction (hHCR). In this strategy, DNA hairpins used for HCR amplification are modified on the four vertexes of TDN, which are then used to perform rapid TDN-hHCR in the presence of an initiator strand, producing large-sized cross-linked reaction products and thus giving greatly improved fluorescence resonance energy transfer (FRET) signal output. Pb DNAzyme catalyzes the cleavage of the initiator strand, inhibiting the initiation of TDN-hHCR and giving decreased FRET signal. Synergetic signal amplification of Pb DNAzyme-catalyzed cleavage reaction and subsequent TDN-hHCR confers the sensing platform with ultrahigh sensitivity. As low as 0.25 pM Pb can be detected by using either signal "turn-on" or "turn-off" mode. The whole detection process can be finished within 20 min. Strong anti-interference capacity of FRET-based ratiometric detection and high specificity of Pb DNAzyme endow the sensing platform with great practical application potential, which was demonstrated by the accurate detection of Pb in real river water, fruit, vegetable and grain samples.
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http://dx.doi.org/10.1016/j.aca.2020.12.050DOI Listing
February 2021

LncRNA AFAP1-AS1 promotes the metastasis of prostate cancer.

Minerva Med 2021 Jan 19. Epub 2021 Jan 19.

Department of Cardiothoracic Surgery -

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http://dx.doi.org/10.23736/S0026-4806.20.07228-6DOI Listing
January 2021

A phase 1 study of entinostat in children and adolescents with recurrent or refractory solid tumors, including CNS tumors: Trial ADVL1513, Pediatric Early Phase-Clinical Trial Network (PEP-CTN).

Pediatr Blood Cancer 2021 Apr 12;68(4):e28892. Epub 2021 Jan 12.

Department of Pediatrics, University of Minnesota Masonic Cancer Center, Minneapolis, Minnesota.

Background: Entinostat is an oral small molecule inhibitor of class I histone deacetylases (HDAC), which has not previously been evaluated in pediatrics. We conducted a phase I trial to determine the maximum tolerated dose/recommended phase 2 dose (MTD/RP2D), toxicity profile, pharmacokinetics (PK), and pharmacodynamics (PD) of entinostat in children with relapsed or refractory solid tumors including central nervous system (CNS) malignancies.

Methods: A rolling six dose escalation design evaluated two dose levels. Entinostat oral tablet formulation was administered once per week, four doses per 28-day cycle. PK and PD studies were performed.

Results: Twenty-one eligible patients' median (range) age was 14 years (6-20). Six subjects were treated at 3 mg/m dose level and 15 were treated in 4 mg/m dose level. The study included patients with CNS tumors (n = 12), sarcomas (n = 6), or other solid tumors (n = 3). Eight patients were not fully evaluable for toxicity due to progression of disease prior to receiving the required percentage of protocol therapy. No cycle one dose-limiting toxicity (DLT) was observed at either dose level. A three-fold higher area under the curve (AUC) was achieved in our cohort compared to adults using a similar dosing schedule. The PD studies showed increase in acetylated lysine in peripheral blood leukocytes at both doses.

Conclusions: Entinostat was well tolerated with no DLT observed. All patients experienced progression within the first two cycles, except one patient with ependymoma with stable disease. Based on PK and PD, the R2PD in pediatric patients with solid tumors is 4 mg/m orally administered once weekly.
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http://dx.doi.org/10.1002/pbc.28892DOI Listing
April 2021

Intrahepatic cholestasis of pregnancy is associated with an increased risk of gestational diabetes and preeclampsia.

Ann Transl Med 2020 Dec;8(23):1574

Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, Zhengzhou, China.

Background: Previous studies have shown that intrahepatic cholestasis of pregnancy (ICP) is associated with an increased incidence of adverse perinatal outcomes, and this supports the contention that ICP is associated with increased risk for both gestational diabetes mellitus (GDM) and preeclampsia. The purpose of this study was to review adverse maternal and perinatal outcomes of ICP in the Chinese population, and to investigate the association between ICP and GDM, as well as between ICP and preeclampsia.

Methods: We conducted a retrospective cohort study in which we compared pregnancies affected by ICP with all other deliveries during the study period. Data from women with singleton pregnancies who delivered in 14 representative hospitals in China between October 1, 2016 and September 30, 2017 were collected from our database system. We then performed logistic regression analysis to determine the odds ratios (OR) and 95%CIs of the adverse pregnancy outcomes among women with or without ICP.

Results: A total of 95,728 singleton births were included in the study, and among these, 911 pregnancies were diagnosed as having ICP, resulting in an incidence of 0.95%. Women with ICP were more likely to have GDM [adjusted odds ratio (aOR), 1.406; 95% CI, 1.179-1.677; P<0.001] and preeclampsia (aOR, 2.241; 95% CI, 1.678-2.992; P<0.001) compared with those who did not have ICP. Women in the ICP group exhibited higher rates of scheduled cesarean deliveries (aOR, 3.527; 95% CI, 2.981-4.173; P<0.001) and cesarean deliveries during labor (aOR, 4.388; 95% CI, 1.815-10.612; P=0.027). Women with ICP were also more likely to have iatrogenic preterm delivery (aOR, 2.449; 95% CI, 1.92-3.122; P<0.001) and admission to the neonatal intensive care unit (aOR, 1.572; 95% CI, 1.318-1.874; P<0.001). There was no increased risk of stillbirth in the cohort of ICP cases (aOR, 0.430; 95% CI, 0.049-3.767; P=0.259).

Conclusions: ICP was associated with an increased risk of GDM and preeclampsia in singleton pregnancies. Pregnancies with ICP therefore have significantly increased risks of adverse perinatal outcomes.
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http://dx.doi.org/10.21037/atm-20-4879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791254PMC
December 2020

Pregnancy outcomes of Chinese women undergoing IVF with embryonic cryopreservation as compared to natural conception.

BMC Pregnancy Childbirth 2021 Jan 9;21(1):39. Epub 2021 Jan 9.

Department of Minimally Invasive Gynecologic Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, No. 17 Qihelou Road, Dong Cheng District, Beijing, China.

Background: To examine differences in the maternal characteristics and pregnancy outcomes of Chinese women with various causes of infertility who underwent in vitro fertilization (IVF) with embryonic cryopreservation treatment.

Methods: Cases were pregnancies after IVF-ET with embryonic cryopreservation; controls were spontaneously conceived pregnancies. Subgroup analysis was carried out according to etiology of infertility. The IVF treatment group was divided into 5 subgroups according to infertility etiology as follows: ovulation disorder, tubal disease, male infertility, endometriosis, and mixed infertility. Data on demographic characteristics, medical history, laboratory tests, and delivery were reviewed. Logistic regression analysis was performed for pregnancy and perinatal complications and neonatal outcomes. The multivariable model was adjusted for potential confounders.

Results: Among singleton pregnancies, compared with spontaneous pregnancies, IVF pregnancies were associated with significant increases in the rates of the following: gestational diabetes mellitus (GDM) (aOR 1.76[95% CI 1.33-2.33]), preeclampsia (2.60[1.61-4.20]), preterm preeclampsia (4.52[2.03-10.06]), postpartum hemorrhage (1.57[1.04-2.36]), intrahepatic cholestasis of pregnancy (3.84[1.06-13.94]), preterm premature rupture of membranes (2.11[1.17-3.81]), preterm birth (1.95[CI 1.26-3.01]), low birthweight (1.90[1.13-3.20]), macrosomia (1.53[1.03-2.27]), and neonatal intensive care unit (NICU) admission (1.69[1.22-2.34]) in the ovulation disorder group; GDM (1.50[1.21-1.86]), placenta previa (2.70[1.59-4.59]), placenta accreta (1.78[1.10-2.89]), postpartum hemorrhage (1.61[1.19-2.18]), macrosomia (1.60[1.21-2.13]) and 5-min Apgar score ≤ 7 (4.09[1.04-16.08]) in the tubal disease group; placenta previa (9.33[4.22-20.62]), small for gestational age (2.29[1.04-5.08]), macrosomia (2.00[1.02-3.95]) and NICU admission (2.35[1.35-4.09]) in the endometriosis group; placenta previa (4.14[2.23-7.68]) and placenta accreta (2.05[1.08-3.87]) in the male infertility group; and GDM (1.85[1.15-2.98]), placenta previa (4.73[1.83-12.21]), placental abruption (3.39[1.20-9.56]), chorioamnionitis (2.93[1.04-8.26]), preterm birth (2.69[1.41-5.15]), and 1-min Apgar score ≤ 7 (4.68[1.62-13.51]) in the mixed infertility group. Among multiple pregnancies, most of the differences that were significant in singleton pregnancies were less extensive or had disappeared.

Conclusions: Infertility etiology within the IVF population was found to affect maternal and neonatal outcomes among all births. During the perinatal period, infertility etiology appears to be an additional risk factor for abnormal pregnancy outcomes besides the use of IVF techniques compared with spontaneous pregnancies. Higher risk was found for ovulation disorders, and lower risk was found for male infertility.
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http://dx.doi.org/10.1186/s12884-020-03486-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796545PMC
January 2021

A novel ISM-SAM strategy, based on gas chromatography/mass spectrometry analysis, to compensate for matrix effects in the determination of pyruvic acid.

Rapid Commun Mass Spectrom 2021 Apr;35(7):e9042

Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, P.R. China.

Rationale: The matrix effect is tricky in gas chromatography/mass spectrometry (GC/MS) analyses. Although several methods have been proposed to solve this problem, the results were unsatisfactory. Even fewer studies have assessed the performance of corrective methods. Hence, our study focused on assessing several common corrective methods, and then proposed a new strategy to correct for the matrix effect in GC/MS analyses.

Methods: In GC/MS analyses, the internal standard method (ISM) was employed to overcome the matrix effect during the detection of pyruvic acid (PA) in serum samples from a healthy adult female. The accuracy of the ISM was evaluated by comparing it with the standard addition method (SAM). To employ the ISM-SAM strategy, correction factors (CFs) were established by combining the ISM and the SAM based on different groups. The CFs were used to normalize data onto the results of subsequent analyses.

Results: When using the ISM to detect levels of PA, a serious bias is observed, thereby affecting the conclusions reached. In contrast, more reliable data can be obtained after normalizing results by undertaking the ISM-SAM strategy. The feasibility of this strategy was verified by comparing it with the results of the SAM alone. The ISM-SAM strategy was successfully applied to quantify the PA levels in healthy people and nephrotic syndrome patients.

Conclusions: Our results indicated that a false outcome was presented when only the ISM was used to adjust the data, and important information would be missed if the correction strategy was not carried out. Therefore, ISM-SAM, as an available correction method, should be adapted to improve the reliability of research results.
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http://dx.doi.org/10.1002/rcm.9042DOI Listing
April 2021