Publications by authors named "Xiaotao Shi"

12 Publications

  • Page 1 of 1

Can feeding sound attract flower fish (Ptychobarbus kaznakovi)?

J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2021 Jun 24. Epub 2021 Jun 24.

Hubei International Scientific and Technological Cooperation Center of Ecological Protection and Management, Three Gorges Area, China Three Gorges University, Yichang, Hubei Province, 443002, People's Republic of China.

The use of acoustic attractants may have the potential to guide native migratory species towards safe passage. Flower fish Ptychobarbus kaznakovi, a short-distance migratory fish whose population is in decline in the past decades, was exposed to three acoustic stimuli (feeding sound, ambient riverine noise and the pure tone 1000 Hz) to examine the phonotaxic responses using playbacks approaches in a fibreglass tank. The results showed that the flower fish showed significantly greater positive phonotaxis and swam towards the sound sources significantly faster in response to the feeding sounds than to ambient riverine noise and the pure tone during the 5-min exposure. Distribution experiments were conducted to study the preference of flower fish to the three sounds stimuli. The results showed that the experimental fish in feeding sound trials spent significant more time in areas closer to the sound sources than that in the pure tone and the ambient riverine noise trials, respectively. This study indicates that the feeding sounds may serve as potential acoustic attractants to guide flower fish to safe passage routes.
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http://dx.doi.org/10.1007/s00359-021-01501-6DOI Listing
June 2021

[Short-term effectiveness of arthroscopic single bundle four-strand reconstruction using autologous semitendinosus tendon and anterior half of peroneus longus tendon for posterior cruciate ligament injuries].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 May;35(5):556-561

Department of Knee Surgery, Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, 471002, P.R.China.

Objective: To investigate the short-term effectiveness of arthroscopic single bundle four-strand reconstruction using autologous semitendinosus tendon and anterior half of peroneus longus tendon for posterior cruciate ligament (PCL) injuries.

Methods: A clinical data of 30 patients with PCL injury, who were admitted between December 2015 and September 2018 and met the selection criteria, was retrospectively analyzed. All patients were treated with arthroscopic single bundle four-strand reconstruction using autologous semitendinosus tendon and anterior half of peroneus longus tendon and TightRope technique. Among them, 19 were male and 11 were female, aged 17-48 years (mean, 28.2 years). The PCL injury was caused by traffic accident in 8 cases, sport in 14 cases, falling and bruising by a heavy objective in 5 cases, and other injuries in 3 cases. The interval between injury and operation was 10-90 days (mean, 39.3 days). The PCL injury was rated as grade Ⅱ in 6 cases and grade Ⅲ in 24 cases. The posterior drawer test was positive in 26 cases and the inverse Lachman test was positive in 24 cases. The International Knee Documentation Committee (IKDC) score was 61.37±8.49, and the objective IKDC ligament grading was near normal in 2 cases, abnormal in 8 cases, and significantly abnormal in 20 cases. The modified Lysholm knee score was 62.20±5.67. The knee range of motion (ROM) was (101.83±8.15) °.

Results: The operative time ranged from 70 to 110 minutes (mean, 79.7 minutes). All incisions healed by first intetion. All patients were followed up 12-24 months (mean, 19.0 months). There were 3 cases of deep vein thrombosis in the lower extremity after operation, and 1 case of approximately 10° limitation of knee extension. At last follow-up, the posterior drawer test was positive in 2 cases and the inverse Lachman test was positive in 1 case, with significant differences compared with the preoperative period ( =38.571, =0.000; =36.274, =0.000). The IKDC score was 84.67±3.67, and the objective IKDC ligament grading was normal in 16 cases, nearly normal in 10 cases, abnormal in 3 cases, and significantly abnormal in 1 case; the modified Lysholm knee score was 90.37±4.49; all of the above indexes were significantly better than preoperative ones, and the differences were significant ( =-12.387, =0.000; =-2.810, =0.005; =-22.865, =0.000). Knee ROM was (88.33±9.86)° at 1 month after operation and reached (113.33±13.48)° at last follow-up, showing significant differences between pre- and post-operation ( <0.05). MRI re-examination showed that the form and position of reconstructed PCL were satisfactory.

Conclusion: It can obtain good short-term effectiveness for PCL injuries by arthroscopic single bundle four-strand reconstruction using autologous semitendinosus tendon and anterior half of peroneus longus tendon, which has the advantages of reliable surgical approach, safe operation, and precise effectiveness.
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http://dx.doi.org/10.7507/1002-1892.202011058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175203PMC
May 2021

Evaluation of Accuracy of Preoperative Planning of the Femurofibular Angle in Open-Wedge High Tibial Osteotomy for Mild Medial Knee Osteoarthritis.

Biomed Res Int 2021 18;2021:8813300. Epub 2021 Feb 18.

Department of Knee Surgery, Luoyang Orthopedic-Traumatological Hospital (Henan Orthopedic Hospital), Qiming Southern Road, Luoyang, Henan 471002, China.

Objective: The purpose of this study was to evaluate the usefulness of preoperative planning of the femurofibular angle (FFA) in medial open-wedge high tibial osteotomy (OWHTO) for mild medial knee osteoarthritis.

Methods: Thirty-two patients (32 knees) with mild medial knee OA were retrospectively reviewed. The patients underwent preoperative planning of the FFA for OWHTO. For preoperative planning, a full-length weight-bearing X-ray photograph of the lower limb was opened within Adobe Photoshop Software, and a targeted corrective mechanical axis line of the lower limb and its intersecting point at the lateral tibial plateau surface was drawn using rectangle selection and filling tools. A frame, which encircled the tibia and fibula, was created around the predicted osteotomy plane and then rotated until the ankle center was on the targeted mechanical axis line. Subsequently, a distal femoral condyle line and a proximal fibula axis line were drawn, and the angle between the two lines was measured and defined as the femurofibular angle (FFA). During biplane OWHTO, the preoperatively determined FFA was used to complete the correction of the mechanical axis. During follow-up, the postoperative mechanical weight-bearing line (WBL) of the lower limb, the mechanical femorotibial angle (mFTA), and the FFA were measured and compared with the preoperatively determined values.

Results: The mechanical WBL shifted from a preoperative value of 25.36 ± 5.02% to a postoperative value of 56.19 ± 0.10% from the medial border along the mediolateral width of the tibial plateau, and it was 56.57 ± 0.08% at the final follow-up ( < 0.01). The preoperatively determined value was 56.25%, and no significant difference was found compared with postoperative week-one and final follow-up values ( > 0.05). The mFTA was corrected from a preoperative varus of 4.02 ± 0.63° to a postoperative week-one valgus of 2.37 ± 0.28°, and it had a valgus of 2.48 ± 0.39° at the final follow-up ( < 0.01). No significant difference in the valgus was found compared with the postoperative week-one, final follow-up and preoperatively determined valgus of 2.34 ± 0.26° ( > 0.05). The postoperative week-one and final follow-up FFAs were 90.34 ± 1.53° and 90.33 ± 1.52°, respectively, and no significant difference was found compared with the preoperatively determined value of 90.12 ± 1.72° and the intraoperative setting value of 90.25 ± 1.67° ( > 0.05). All corrected values were within the acceptable range of preoperative planning.

Conclusion: Preoperative planning of the FFA may be useful in OWHTO for patients with mild medial knee OA. Satisfactory correction of the postoperative targeted mechanical axis line of the lower limb can be obtained.
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http://dx.doi.org/10.1155/2021/8813300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985241PMC
May 2021

Inhibitory properties of Chinese Herbal Formula SanHuang decoction on biofilm formation by antibiotic-resistant Staphylococcal strains.

Sci Rep 2021 Mar 30;11(1):7134. Epub 2021 Mar 30.

Henan Orthopedic Institute, Henan Luoyang Orthopedic-Traumatological Hospital (Henan Orthopedic Hospital), Qiming Southern Road, Luoyang, 471002, Henan, People's Republic of China.

The aim of this study was to explore the effect of Chinese herbal SanHuang decoction (SH) on biofilm formation of antibiotic-resistant Staphylococci on titanium surface, and to explore its mechanism. Biofilm-forming ATCC 35984, ATCC 43300 and MRSE 287 were used in this study. The MICs of SH and vancomycin against Staphylococci were determined by the broth microdilution method. Six groups were designed, namely control group (bacteria cultured with medium), 1/8MIC SH group (1MIC SH was diluted by 1/8 using TSB or saline), 1/4MIC SH group, 1/2MIC SH group, 1MIC SH group and vancomycin group (bacteria cultured with 1MIC vancomycin). The inhibitory effect on bacterial adhesion and biofilm formation were observed by the spread plate method, CV staining, SEM, and CLSM. Real-time PCR was performed to determine the effect of SH on the expression levels of ica AD and ica R gene in ATCC 35984 during the biofilm formation. The strains were found to be susceptible to SH and vancomycin with MIC of 38.75 mg/ml and 2.5 μg/ml, respectively. SH with 1 MIC and 1/2 MIC could inhibit the bacteria adhesion, showing only scattered adhesion from SEM. CLSM showed that SH with 1 MIC and 1/2 MIC inhibited the biofilm formation. The quantitative results of the spread plate method and CV staining showed that there was significant differences between the SH groups (P < 0.05). Further, with an increase in SH concentration, the inhibitory effect became more obvious when compared with control group. Among the groups, vancomycin had the strongest inhibitory effect on bacterial adhesion and biofilm formation (P < 0.01). With an increase in SH concentration, the expression levels of ica AD decreased, and the expression of ica R increased correspondingly (P < 0.05). In conclusions, SH can inhibit the biofilm formation of antibiotic-resistant Staphylococci. Its probable mechanistic activity may be through the inhibition of polysaccharide intercellular adhesin synthesis by down-regulating the expression of ica AD gene.
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http://dx.doi.org/10.1038/s41598-021-86647-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009868PMC
March 2021

In vivo visualization assay to evaluate the effects of maternal exposure to mercury on offspring bioaccumulation in the oriental river prawn (Macrobrachium nipponense).

Chemosphere 2021 May 28;270:129440. Epub 2020 Dec 28.

Hubei International Science and Technology Cooperation Base of Fish Passage, China Three Gorges University, Yichang, 443002, China.

Mercury (Hg) is a persistent pollutant that accumulates in aquatic animals. However, studies related to understand how gonad tissue of this species responds to mercury exposure and elucidation of mercury bioaccumulation in crustacean offspring by cross-generational, are still sparse. The present study aimed to assess the bioaccumulation of Hgin vivo in prawn offspring by a specific aggregation-induced emission fluorogen (AIEgen). The 96 h median lethal concentration (LC) values of mercury to the juveniles were 0.072 mg/L. Hg reduced growth performance, damaged oocyte quality, and inhibited ovary maturation, thus inhibiting gonadal maturation in intact prawns. F1 offspring were exposed to Hg by direct transfer from their F0 parents, as shown by the distribution of mercury in gonads and fertilized eggs. In the medium containing oriental river prawn larvae, the Hg concentration decreased rapidly, indicating fast initial larval uptake of Hg. Due to metal ion triggered AIE activity, analysis of fluorescence images showed that prawn offspring accumulated Hg via maternal transfer, and there was a relationship among the photoluminescence intensity, the AIEgen concentration, and mercury levels. The quantitative detection of Hg absorption from prawn larvae by the AIEgen represents a novel analytical technique to understand the dynamics of Hg between maternal and offspring.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129440DOI Listing
May 2021

Susceptibility of Mature Biofilms to Chinese Herbal Decoction Sanhuang Jiedu: An Study.

Biomed Res Int 2020 28;2020:7473942. Epub 2020 Sep 28.

Henan Orthopedic Institute, Henan Luoyang Orthopedic-Traumatological Hospital (Henan Orthopedic Hospital), Luoyang 471000, China.

Background: External socking and washing with the Chinese herbal Sanhuang Jiedu decoction (SHJD) can effectively control local limb infections with bone and implant exposure. However, the antibiofilm activities of this decoction have not yet been investigated. Therefore, the aim of this study was to examine the effects and characteristics of SHJD on the mature biofilms of multidrug-resistant on a titanium surface.

Methods: Biofilm-forming methicillin-resistant 35984 and 43330, and non-biofilm-forming 12228 were selected as the experimental strains. The mature biofilms were prepared on titanium surfaces. The five experimental groups were based on dilution concentrations (DC) of SHJD: the control group (biofilm incubated with 0.85% NaCl solution), the SHJD (DC:1/8) group (initial SHJD solution was diluted 1/8), the SHJD (DC:1/4) group, the SHJD (DC:1/2) group, and the SHJD (DC:1/1) group (initial SHJD solution). The effects of SHJD on the mature biofilms were observed with the bacterial spread plate method, crystal violet (CV) staining, scanning electron microscopy, and confocal laser scanning microscopy.

Results: After culture in tryptic soy broth for 72 h, 43300 and 35984 produced mature biofilms and 12228 did not. The optical density value of 12228 was 0.11 ± 0.02, significantly lower than that of 35984 (0.42 ± 0.05) or 43300 (0.41 ± 0.03) ( < 0.05). The mature biofilms of 43300 and 35984 clearly disintegrated when incubated for 12-24 h with SHJD (DC:1/1) or SHJD (DC:1/2), showing only scattered bacterial adhesion. In the SHJD (DC:1/4) group, although many residual bacterial colonies still clustered together, presenting a biofilm structure, it was very looser than that in the SHJD (DC:1/8) group in which the biofilm was similar to that in the control group. For 12228, only colony adhesion was observed, and the number of colonies decreased as the concentration of SHJD or the culture period increased. The quantitative results for the bacterial spread plate and CV staining showed significant differences between the SHJD groups ( < 0.05).

Conclusion: SHJD has antibiofilm activity against multidrug-resistant strains. It weakens or disrupts already-formed mature biofilms on titanium surfaces in a concentration- and incubation time-dependent manner.
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http://dx.doi.org/10.1155/2020/7473942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539100PMC
May 2021

IPM strategies to a discrete switching predator-prey model induced by a mate-finding Allee effect.

J Biol Dyn 2019 12;13(1):586-605

College of Science, Hubei Minzu University, Enshi, People's Republic of China.

This paper proposes a discrete switching predator-prey model with a mate-finding Allee effect, where also switches are guided by Allee effect. One of the strategies analysed is to use a chemical in order to prevent the pest outbreak when the pest population is free of Allee effect. In this paper, we first study analytically the dynamic behaviors of the two subsystems and the equilibria and their stability of the switched system. Then we provide numerical bifurcation analyses for the switched discrete system. These show that the switched discrete system may have very complex dynamics by 2-parameter bifurcation diagrams which divide the space into regions and study equilibria, and 1-dimensional bifurcation diagrams which reveal that the system has periodic, chaotic solutions, period doubling bifurcations and so on. Furthermore, we try to refer the key parameters and initial densities of both populations associated with pest outbreaks and study their biological implications.
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http://dx.doi.org/10.1080/17513758.2019.1682200DOI Listing
December 2019

Retinal-based polyene fluorescent probe for selectively detection of Cu in physiological saline and serum.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Feb 20;227:117565. Epub 2019 Oct 20.

College of Biological and Pharmaceutical Sciences, Engineering Research Center of Eco-environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, Hubei, 443002, PR China. Electronic address:

Retinal is a flexible natural chromophore and widely present in organisms. The slender conjugated polyene structure retinal is conducive to entering protein structure. In this work, a novel turn-on fluorescent probe for Cu based on retinal and phenylenediamine was designed and synthesized. The probe achieved recognition of copper ions in human serum complex protein environment. Furthermore, the high sensitivity, selectivity for Cu and the sensing mechanism was also investigated.
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http://dx.doi.org/10.1016/j.saa.2019.117565DOI Listing
February 2020

Polystyrene microplastics alter the behavior, energy reserve and nutritional composition of marine jacopever (Sebastes schlegelii).

J Hazard Mater 2018 10 3;360:97-105. Epub 2018 Aug 3.

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. Electronic address:

Uptake and toxicity of microplastics on marine organisms have been reported elsewhere. However, there is limited knowledge regarding the ecological effects of microplastics on marine organisms. In this study, we investigated the effects of polystyrene microplastics (1 × 10 microspheres per L) on the behavior, energy reserve and nutritional composition of juvenile jacopever (Sebastes schlegelii). Compared to the controls, fish treated by microplastics showed lower sensitivity toward the added food in the tank, and increased foraging time, indicating that microplastics significantly weakened feeding activity of the fish. Interestingly, the microplastics treated-fish obviously reduced swimming speed and range of movement, demonstrating that polystyrene microplastics could have negative effect on hunting behavior. Furthermore, polystyrene microplastics accumulated in the gills and intestine, causing significant histopathological changes in the gallbladder and liver. Moreover, the energy reserve and nutritional quality of fish were influenced by microplastics as evidenced by lower growth, protein and lipid contents. Our results highlighted the potential negative effects of microplastics on marine ecological function and food safety.
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http://dx.doi.org/10.1016/j.jhazmat.2018.07.110DOI Listing
October 2018

The bioaccumulation of fluoride ion (F(-)) in Siberian sturgeon (Acipenser baerii) under laboratory conditions.

Chemosphere 2009 Apr 17;75(3):376-80. Epub 2009 Jan 17.

School of Life Science, East China Normal University, Shanghai 200062, China.

A 90-d growth trial was conducted to determine the accumulation of fluoride ions (F(-)) in juvenile Siberian sturgeon (Acipenser baerii). Fish were exposed to nominal F(-) concentrations of 4, 10, 25, 62.5 mg L(-1) (added as NaF), along with a control group. Fish growth was determined and F(-) concentrations in bone, cartilage, skin, gill, muscle, liver, gut and pylorus were analyzed by fluoride specific electrode method. Fish growth was inhibited for groups exposed to 10, 25, and 62.5 mg F(-) L(-1) compared to the control group. Significant increases in F(-) concentrations were observed in bone, cartilage, skin and gill of Siberian sturgeon exposed to 4 mg F(-) L(-1). The highest F(-) concentration was in bone (3204.4 mg kg(-1), dry weight), followed by cartilage (1401.2 mg kg(-1)), gill (389.4 mg kg(-1)), skin (100.1 mg kg(-1)). Muscle, liver, gut and pylorus did not show increasing accumulation with increasing F(-) levels in the water, but liver, gut, pylorus accumulated higher F(-) concentration compared to muscle. It is summarized that F(-) accumulates mainly in bone and cartilage in Siberian sturgeon, with 10 mg F(-) L(-1) in water harmful to growth. Even 4 mg F(-) L(-1) may be harmful to Siberian sturgeon growth during a long-term waterborne exposure.
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http://dx.doi.org/10.1016/j.chemosphere.2008.12.042DOI Listing
April 2009

[Effects of rearing density on juvenile Acipenser schrenckii digestibility, feeding rate and growth].

Ying Yong Sheng Tai Xue Bao 2006 Aug;17(8):1517-20

College of Fishery, Huazhong Agricultural University, Wuhan 430070, China.

A 60-day rearing experiment was conducted in this study. The initial weight of juvenile Acipenser schrenckii was 43.90 +/- 1.75 g, and the initial rearing density was 0.525 (LSD), 1.171 (MSD), and 2.138 (HSD) kg x m(-2), respectively. The results showed that with increasing rearing density, the specific growth rate (SGR) and daily weight gain (DWG) of juvenile A. schrenckii were significantly decreased, while net yield (NY) was increased. The digestibility was significantly higher in LSD and MSD than in HSD, while that in LSD and MSD was nearly the same. The feeding rate in MSD was significantly-lower than that in HSD, but slimly higher than that in LSD. There was a significant negative linear correlation between food conversion ratio (FCR) and digestibility, and a significant positive linear correlation between SGR and digestibility.
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August 2006

Comparative blood biochemistry of Amur sturgeon, Acipenser schrenckii, and Chinese surgeon, Acipenser sinensis.

Fish Physiol Biochem 2006 Mar;32(1):63-6

College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.

This study compared Amur sturgeon (Acipenser schrenckii) and Chinese sturgeon (Acipenser sinensis) on several biochemical parameters including glucose (GLU), urea, lactate dehydrogenase (LDH-L), activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct bilirubin (D.BILI), alkaline phosphatase (ALP), cholesterol (CHOL), triglyceride (TGL), creatinine (CREA), total protein (TP), and total bilirubin (T.BILI). The results showed that there were great differences in these parameters between the two species. The concentrations of GLU, UREA, LDH-L, ALT, AST, D.BILI, ALP, CHOL, TGL, CREA, and T.P for the Amur sturgeon were significantly higher than those for Chinese sturgeon.The T.BILI level of the Amur sturgeon was, however, significantly lower than that for the Chinese sturgeon.
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http://dx.doi.org/10.1007/s10695-006-7134-9DOI Listing
March 2006
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