Publications by authors named "Xiaorong Han"

15 Publications

  • Page 1 of 1

Characteristics and Management of Granulomatous Lobular Mastitis Associated with Antipsychotics-Induced Hyperprolactinemia.

Authors:
Chunxiang Tian Han Wang Zeyu Liu Xiaorong Han Ping Ning

Breastfeed Med 2022 Apr 21. Epub 2022 Apr 21.

Department of Breast Surgery and Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Granulomatous lobular mastitis (GM) is a rare inflammatory breast disease. Reports focusing on GM caused by antipsychotic-induced hyperprolactinemia (HPRL) are very rare. To report a study of GM associated with antipsychotic-induced HPRL and discuss the mechanism and management. A retrospective review of patients with GM and psychiatric disorders were carried out. The clinical characteristics, management and outcome were collected and analyzed. The relationship between antipsychotics and GM was evaluated using the Naranjo Adverse Drug Reaction Probability Scale (Naranjo scale). Nineteen female GM patients with psychiatric diseases, aged 21-39 years, who had received antipsychotics for 0.5-10.2 years were included. Most patients took multiple antipsychotics, and 10 (52.6%) took risperidone-containing regimens. Increased prolactin (PRL) was detected in all patients (range 35.15-200 ng/mL). The scores of Naranjo scale were 7-8, indicated the antipsychotics probably induced GM. All patients received systemic therapy, and were prescribed bromocriptine. Seven patients (36.8%) decreased the dose of antipsychotics, six (31.6%) switched antipsychotics, three (15.8%) continued the primary antipsychotics, and three (15.8%) discontinued antipsychotics. In addition, 14 patients (73.7%) received corticosteroid, 4 (21.1%) received antimycobacterials. PRL decreased to normal in 1 month. Seven patients (36.8%) received excisional surgery. After 12 months' follow-up (range 9-56 months), only three patients (15.8%) had a recurrence. Long-term use of antipsychotics may increase PRL levels, and lead to GM. It is vital to assess PRL level and reduce PRL to normal in patients with GM.
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http://dx.doi.org/10.1089/bfm.2021.0341DOI Listing
April 2022

Cardio-Oncology: A Myriad of Relationships Between Cardiovascular Disease and Cancer.

Authors:
Yinghui Wang Yonggang Wang Xiaorong Han Jian Sun Cheng Li Binay Kumar Adhikari Jin Zhang Xiao Miao Zhaoyang Chen

Front Cardiovasc Med 2022 17;9:727487. Epub 2022 Mar 17.

Department of Cardiology, Union Hospital, Fujian Medical University, Fuzhou, China.

Cardiovascular disease (CVD) and cancer are the leading causes of death worldwide. With an increasing number of the elderly population, and early cancer screening and treatment, the number of cancers cases are rising, while the mortality rate is decreasing. However, the number of cancer survivors is increasing yearly. With the prolonged life span of cancer patients, the adverse effects of anti-tumor therapy, especially CVD, have gained enormous attention. The incidence of cardiovascular events such as cardiac injury or cardiovascular toxicity is higher than malignant tumors' recurrence rate. Numerous clinical studies have also shifted their focus from the study of a single disease to the interdisciplinary study of oncology and cardiology. Previous studies have confirmed that anti-tumor therapy can cause CVD. Additionally, the treatment of CVD is also related to the tumors incidence. It is well established that the increased incidence of CVD in cancer patients is probably due to an unmodified unhealthy lifestyle among cancer survivors or cardiotoxicity caused by anti-cancer therapy. Nevertheless, some patients with CVD have a relatively increased cancer risk because CVD and malignant tumors are highly overlapping risk factors, including gender, age, hypertension, diabetes, hyperlipidemia, inflammation, and obesity. With advancements in the diagnosis and treatment, many patients simultaneously suffer from CVD and cancer, and most of them have a poor prognosis. Therefore, clinicians should understand the relationship between CVD and tumors, effectively identify the primary and secondary prevention for these diseases, and follow proper treatment methods.
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http://dx.doi.org/10.3389/fcvm.2022.727487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8968416PMC
March 2022

Prognostic significance of QRS distortion and frontal QRS-T angle in patients with ST-elevation myocardial infarction.

Authors:
Xiaorong Han Zhongbo Chen Yinghui Wang Jin Zhang Ying Zhang Qiang Su Zhenghu Pan Jian Sun Yonggang Wang

Int J Cardiol 2021 12 27;345:1-6. Epub 2021 Oct 27.

Department of Cardiovascular Center, Jilin University First Hospital, China. Electronic address:

Background: QRS distortion (G3I) and frontal QRS-T angle (fQRS-T angle) are both electrocardiographic (ECG) signs of ongoing ischemia and depolarization-repolarization heterogeneity, which always occur in patients with ST-segment elevation acute myocardial infarction (STEMI).

Methods: We retrospectively collected 592 STEMI patients who underwent coronary angiography and follow-up for 42 months. 1. We divided the patients into two groups according to whether they had G3I on admission, compared the differences in examination data and endpoint events between these two groups. 2. Group patients according to whether the endpoint events happened in hospital, at 12 and 42 months, compare whether there is a difference in fQRS-T angle at the same time point, and find out the predictive cutoff value of all-cause death. 3. Combined G3I and fQRS-T angle together to enhance the predictive value.

Results: G3I and fQRS-T angle are both independent risk factors for all-cause death in STEMI patients within 12 months (G3I P = 0.014, fQRS-T angle P < 0.001) and within 42 months (P < 0.001). The cutoff values of fQRS-T angle for predicting all-cause death are 66.5° at 12 months and 90.5° at 42 months. When G3I and fQRS-T angle are combined used to predict the mortality, the specificity is significantly improved, but the sensitivity decreased.

Conclusions: G3I and fQRS-T angles are valuable in the prognostic assessment of STEMI patients, especially when combined. These findings help clinicians to identify high-risk patients early for more aggressive treatment.
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http://dx.doi.org/10.1016/j.ijcard.2021.10.139DOI Listing
December 2021

The digestive system involvement of antiphospholipid syndrome: pathophysiology, clinical characteristics, and treatment strategies.

Authors:
Jin Zhang Cheng Li Xiaorong Han Zhongbo Chen Binay Kumar Adhikari Yinghui Wang Yonggang Wang Jian Sun

Ann Med 2021 12;53(1):1328-1339

Department of Cardiovascular Center, Jilin University First Hospital, Changchun, China.

Antiphospholipid syndrome (APS) is an autoimmune disease mainly characterised by vascular thrombosis and pregnancy morbidity. APS has broad spectrum of clinical manifestations. The digestive system involvement of antiphospholipid syndrome is a critical but under-recognised condition. Digestive system involvement may be the result of direct (autoimmune-mediated) or indirect (thrombotic) mechanisms. Liver is the most commonly involved organ, followed by intestines, oesophagus, stomach, pancreas and spleen. This review describes possible digestive system manifestations in APS patients, and illustrates the epidemiology and possible pathophysiology of APS. The role of different treatment strategies in the management of digestive system manifestations of APS were also discussed.Key messagesAntiphospholipid syndrome is a multi-organ, multi-system disease and its clinical manifestation spectrum is gradually expanding. Since the first diagnosis of APS, the clinical manifestations of digestive system have been reported successively. This narrative review describes the major digestive system manifestations of APS and illustrates the epidemiology, pathophysiology and the role of therapeutic strategies of these patients.
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http://dx.doi.org/10.1080/07853890.2021.1962964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381908PMC
December 2021

Congenital absence of the right coronary artery with acute myocardial infarction: report of two cases and review of the literature.

Authors:
Zhongbo Chen Jinhua Yan Xiaorong Han Binay Kumar Adhikari Jin Zhang Ying Zhang Jian Sun Yonggang Wang

J Int Med Res 2020 Dec;48(12):300060520971508

Department of Cardiovascular Centre, The First Hospital of Jilin University, Changchun, Jilin, China.

Congenital absence of the right coronary artery (RCA) is a rare coronary anomaly. Few cases of this condition have been reported. Congenital absence of the RCA is considered as a benign anomaly. However, in certain cases, these patients may develop life-threatening clinical complications that include acute myocardial infarction, stroke, or sudden death. We report two patients who were diagnosed with congenital absence of the RCA and presented with acute myocardial infarction. We discuss our experience in diagnosis and treatment of this disease. Congenital absence of the RCA with acute myocardial infarction is an uncommon clinical emergency. Therefore, early detection, correct diagnosis, and appropriate treatment are important.
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http://dx.doi.org/10.1177/0300060520971508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720338PMC
December 2020

Clinical Analysis of Congenital Deficient Tracheal Cartilage Rings: Six Case Reports and a Literature Review.

Authors:
Jing Ma Chen Meng Zhiyu Feng Xiaorong Han Shuaishuai Liu Na Liu Weiwei Zhu

Front Pediatr 2020 20;8:548089. Epub 2020 Oct 20.

Department of Pediatrics, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Congenital deficiency of tracheal rings is a rare tracheal malformation that can cause central airway obstruction. Herein we reported the clinical data of six patients with symptomatic congenital deficient tracheal rings. There were five cases, with isolated short-segment absent cartilage ring located on the distal trachea (three cases), cervical trachea (one case), and distal trachea combined with bilateral bronchi (one case). Among them, four (4/5) received surgical tracheal resection, three fully recovered, and one died of severe infection. Besides that, one patient, who could not be weaned off the mechanical ventilation, died after rejecting surgery. One case had episodes of recurrent dyspnea and extubation failure due to long-segment tracheomalacia after repair of esophageal atresia and tracheoesophageal fistula. For this patient, deficient cartilage rings were suspected and confirmed at the age of 26 months. Moreover, the clinical characteristics of 12 cases with congenital deficient tracheal cartilage rings reported in previous literature were reviewed. The different characteristics between short- and long-segment deficient cartilage rings were discussed.
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http://dx.doi.org/10.3389/fped.2020.548089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7606407PMC
October 2020

Controlling and optimizing the morphology and microstructure of 3D interconnected activated carbons for high performance supercapacitors.

Authors:
Yanhong Lu Suling Zhang Xiaorong Han Xingchen Wan Junlin Gao Congcong Bai Yingxue Li Zhen Ge Lei Wei Yu Chen Yanfeng Ma Yongsheng Chen

Nanotechnology 2021 Feb;32(8):085401

The Centre of Nanoscale Science and Technology and Key Laboratory of Functional Polymer Materials, State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071, People's Republic of China.

For an active electrode material, the morphology, microstructure and the effective specific surface area derived from them, have a dominant effect for the high performance supercapacitors. In this study, 3D interconnected activated carbons with controlled and optimized morphologies and porous structures were prepared from accessible carbon source and graphene oxide by a hydrothermal carbonization and following an activation method. Through optimizing the ratios of the precursors and reaction conditions, an electrode material with excellent specific surface area of 2318 m g, meso-/macro-pore ratio of 63.2% (meso-/macro-pore volume reached to 0.83 cm g), as well as an outstanding electrical conductivity of 46.6 S m, was obtained. The materials exhibit superior double-layer capacitive performances on a symmetric supercapacitor, delivering superior specific capacitance of 157 F g in organic electrolyte system at current density of 0.5 A g, excellent energy density of 37.6 W h kg with a power density of 7.1 kW kg and good cycling stability of capacitance retention of 94% over 7000 cycles. These results offer a practical method to prepare the desired carbon electrode materials with controlled morphology and structure for high efficiency electrochemical energy storage devices.
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http://dx.doi.org/10.1088/1361-6528/abc98dDOI Listing
February 2021

Why thromboembolism occurs in some patients with thrombocytopenia and treatment strategies.

Authors:
Xiaorong Han Cheng Li Shuai Zhang Xiaojie Hou Zhongbo Chen Jin Zhang Ying Zhang Jian Sun Yonggang Wang

Thromb Res 2020 12 8;196:500-509. Epub 2020 Oct 8.

Department of Cardiovascular Center, Jilin University First Hospital, China. Electronic address:

Platelets play such an important role in the process of thrombosis that patients with thrombocytopenia generally have an increased risk of bleeding. However, abnormal thrombotic events can sometimes occur in patients with thrombocytopenia, which is unusual and inexplicable. The treatments for thrombocytopenia and thromboembolism are usually contradictory. This review introduces the mechanisms of thromboembolism in patients with different types of thrombocytopenia and outlines treatment recommendations for the prevention and treatment of thrombosis. According to the cause of thrombocytopenia, this article addresses four etiologies, including inherited thrombocytopenia (Myh9-related disease, ANKRD26-associated thrombocytopenia, Glanzmann thrombasthenia, Bernard-Soulier syndrome), thrombotic microangiopathy (thrombotic thrombocytopenic purpura, atypical hemolytic uremic syndrome, hemolytic uremic syndrome, Hemolysis Elevated Liver enzymes and Low Platelets syndrome, disseminated intravascular coagulation), autoimmune-related thrombocytopenia (immune thrombocytopenic purpura, antiphospholipid syndrome, systemic lupus erythematosus), and acquired thrombocytopenia (Infection-induced thrombocytopenia and drug-induced thrombocytopenia, heparin-induced thrombocytopenia). We hope to provide more evidence for clinical applications and future research.
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http://dx.doi.org/10.1016/j.thromres.2020.10.005DOI Listing
December 2020

Cardiac biomarkers of heart failure in chronic kidney disease.

Authors:
Xiaorong Han Shuai Zhang Zhongbo Chen Binay Kumar Adhikari Ying Zhang Jin Zhang Jian Sun Yonggang Wang

Clin Chim Acta 2020 Nov 23;510:298-310. Epub 2020 Jul 23.

Department of Cardiovascular Centre, The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address:

Heart failure remains a continuing threat to patients with chronic kidney disease (CKD). Although various heart failure biomarkers have been applied for early detection, diagnosis and prognosis in CKD, these are easily affected by renal insufficiency thus limiting use in these patients. In this review, the major four groups of heart failure biomarkers are explored. These include those associated with: myocardial stretch, ie, brain natriuretic peptide (BNP), N-terminal pro-BNP (NT-proBNP) and mid-regional proatrial natriuretic peptide (MR-proANP); myocyte injury, ie, high-sensitivity troponin T (hsTnT), heart-type fatty acid-binding protein (H-FABP); fibrosis, matrix remodelling and inflammation, ie, soluble growth stimulating gene 2 (sST2), galectin-3 (Gal-3), growth differentiation factor-15 (GDF-15); and renal function, ie, neutrophil gelatinase-associated lipocalin (NGAL) kidney injury molecule-1 (KIM-1), cystatin C (CysC), urinary sodium and urinary albumin. This review highlights classic heart failure biomarkers with critical values adjusted to glomerular filtration rate, summarizes research progress of new heart failure biomarkers and future research directions. Because diagnostic and prognostic usefulness of a single time point biomarker is limited, biomarkers should be combined and monitored at multiple times for optimal clinical impact.
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http://dx.doi.org/10.1016/j.cca.2020.07.040DOI Listing
November 2020

A study on the relationship between chemotherapy-induced cognitive impairment and age in patients with breast cancer.

Authors:
Zeyu Liu Xiaorong Han Chunxiang Tian Jun Chen Lei Lei Qi Liang Xinlin Lv Shenli Tang Ping Ning

J BUON 2018 Nov-Dec;23(6):1601-1605

Department of Breast Surgery, Chengdu Women's & Children's Central Hospital, 1617 Riyue Ave, Qingyang District, Chengdu, 610091, Sichuan,China.

Purpose: To investigate the correlation of chemotherapy-induced cognitive impairment (CICI) with age in patients with triple-negative breast cancer (TNBC).

Methods: A total of 120 breast cancer patients with different ages and receiving chemotherapy were selected as breast cancer group, and another 120 healthy subjects were enrolled as healthy control group. Breast cancer group included 60 TNBC patients (TNBC group) and 60 patients without TNBC (non-TNBC group). Both breast cancer and healthy control group were further divided into young group (n=40), middle-aged group (n=40) and elderly group (n=40). For TNBC group and non-TNBC group, each age group had 20 patients. Then, mini-mental state examination (MMSE), retrospective memory (RM) and prospective memory (PM) questionnaires were performed separately.

Results: There were statistically significant differences in MMSE, RM and PM scale scores between breast cancer group and healthy control group (p<0.001). In breast cancer group, the MMSE score was negatively correlated with age (r=-0.614, p<0.001), and the RM scale and PM scale scores were positively related to age (r=0.527, 0.439, p<0.001). The differences in MMSE, RM and PM scale scores were statistically significant between TNBC group and non-TNBC group (p<0.05). Moreover, the scores of MMSE, RM scale and PM scale were statistically significant among the young, middle-aged and elderly group in both TNBC group and non-TNBC group (p<0.001). In young group, there were statistically significantly differences in scores of MMSE, RM scale and PM scale between TNBC group and non-TNBC group (p<0.001). In middle-aged and elderly group, the scores of MMSE, PM scale and RM scale also had statistically significant differences between TNBC group and non-TNBC group (p<0.001). Multivariate logistic regression analyses revealed that TNBC [odds ratio (OR)=3.659, p=0.004] and age (OR =1.128, p<0.001) were risk factors for the occurrence of cognitive impairment in patients with breast cancer.

Conclusions: Patients receiving chemotherapy for breast cancer suffer from varying degrees of cognitive impairment. The cognitive impairment in TNBC patients is more severe than that in patients without TNBC, the difference being mainly detected in young patients. In addition, both TNBC and age are risk factors for CICI in breast cancer.
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August 2019

Effective inhibition of Cbf-14 against Cryptococcus neoformans infection in mice and its related anti-inflammatory activity.

Authors:
Changzhong Yu Shanshan Wei Xiaorong Han Hanhan Liu Mengxiao Wang Meiling Jiang Min Guo Jie Dou Changlin Zhou Lingman Ma

Fungal Genet Biol 2018 01 6;110:38-47. Epub 2017 Dec 6.

State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu 210009, PR China. Electronic address:

Cbf-14 (RLLRKFFRKLKKSV), a designed peptide derived from cathelicidin family AMP, has proven to be potent against drug-resistant bacteria. In the present study, we investigated the anti-cryptococcal activity of Cbf-14 in vitro and in a pulmonary infection mouse model. Sensitivity test indicated that Cbf-14 possessed effective antifungal activity against Cryptococcus neoformans with an MIC of 4-16 µg/ml, and killing experiments showed that fungicidal activity was achieved after only 4 h treatment with Cbf-14 at 4× MIC concentrations in vitro. Meanwhile, Cbf-14 was effective at prolonging the survival of infected mice when compared with controls, and significantly inhibited the secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6, suggesting its anti-inflammatory activity against fungal infections. As a positively charged peptide, Cbf-14 was proven to neutralize the negative zeta potential of the fungal cell surface, disrupt the capsule polysaccharide of fungi, and further damage cell membrane integrity. These results were confirmed by flow cytometry analysis of the fluorescence intensity after PI staining, while cell membrane damage could be clearly observed by transmission electron microscopy after Cbf-14 (4× MIC) treatment for 1 h. In addition, Cbf-14 increased the IL-10 levels in cultured RAW 264.7 cells, which were stimulated by C. neoformans infection. The obtained data demonstrated that Cbf-14 could rapidly kill C. neoformans cells in vitro, effectively inhibit C. neoformans induced-infection in mice, and inhibit inflammation in vitro / vivo. Therefore, Cbf-14 could potentially be used for the treatment of fungal infections clinically.
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http://dx.doi.org/10.1016/j.fgb.2017.11.008DOI Listing
January 2018

Clinical analysis of bronchoscopic cryotherapy in 156 pediatric patients.

Authors:
Caiyun Ni Huafeng Yu Xiaorong Han Chen Meng Yanqin Zhang

Pediatr Int 2017 Jan 6;59(1):62-67. Epub 2016 Nov 6.

Department of Respiratory Interventional Radiology, Qilu Children's Hospital of Shandong University, Jinan, China.

Background: The aim of this study was to investigate the efficacy and safety of bronchoscopic cryotherapy for pediatric poor ventilation.

Methods: A total of 156 pediatric patients with airway stenosis and obstruction as well as pulmonary atelectasis received bronchoscopic cryotherapy. Treatment efficacy was evaluated on bronchoscopy Before cryotherapy and after the last cryotherapy treatment. Pulmonary atelectasis was assessed according to range of atelectasis on chest computed tomography (CT), and tracheobronchial stenosis according to pulmonary function, tidal volume analysis and dyspnea index.

Results: The 154 patients with effective treatment had patent airway, smooth mucosa and resolved atelectasis. In the two patients with ineffective treatment, however, the airway was not smooth and the range of pulmonary atelectasis was reduced by <30%. Among the 156 patients, 136 had markedly effective treatment, with CT pulmonary atelectasis recovery >70%. The width of the main airway significantly increased approximately 50% and the tidal volume or capacity increased 100%. Effective treatment was identified in 18 patients (18/156), with CT pulmonary atelectasis area reduced 30-70%. Ineffective treatment was noted in two patients, with CT pulmonary atelectasis area reduced by <30%. No complications were noted in any patients during or after operation. No recurrence was noted on follow up of 2-24 months.

Conclusion: Bronchoscopic cryotherapy is effective and safe for dyspnea caused by airway obstruction, and wide application in clinic is recommended.
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http://dx.doi.org/10.1111/ped.13088DOI Listing
January 2017

Clinical analysis of bronchoscopic electrocoagulation in pediatric patients.

Authors:
Caiyun Ni Huafeng Yu Xiaorong Han Chen Meng Yanqing Zhang

Int J Clin Exp Med 2014 15;7(12):5657-62. Epub 2014 Dec 15.

Department of Respiratory Interventional Radiology, Qilu Children's Hospital of Shandong University Jinan 250022, P. R. China.

This study is to explore the efficacy and safety of bronchoscopic electrocoagulation treatment for pediatric disease of poor ventilation. Seventy pediatric patients of airway stenosis and obstruction as well as pharyngeal and laryngeal cysts received bronchoscopic electrocoagulation treatment, including 15 cases of epiglottic cyst, 13 cases of cicatricial hyperplasia of fibrous tissue after trachea intubation, 5 cases of foreign body in bronchus and 37 cases of endobronchial tuberculosis. Before and after the last electrocoagulation treatment, treatment efficacy was evaluated by examining the patients' clinical presentations and lesions in airway under bronchoscope, examining chest CT and pulmonary function, and estimating pulmonary atelectasis and ventilation function. Seventy cases of pediatric patients were treated by bronchoscopic electrocoagulation, with the total treatment number of 106 times. Among them, 66 cases were treated with marked efficacy and 4 cases were with effective treatment. There was no invalid treatment. The treatment efficacy was 100% without complications. Bronchoscopic electrocoagulation treatment is a fast, effective and safe therapeutic method in treating airway stenosis and obstruction, such as foreign body in bronchus, granulation tissue hyperplasia, and epiglottic cysts. It is worthy of being widely applied in clinic.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307533PMC
February 2015

Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol.

Authors:
Yang Yang Chao Yuan Jie Dou Xiaorong Han Hui Wang Hongqing Fang Changlin Zhou

World J Microbiol Biotechnol 2014 Dec 1;30(12):3263-72. Epub 2014 Oct 1.

School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, People's Republic of China.

Shikimic acid (SA) is an industrially important chiral compound used in diverse commercial applications, and the insufficient supply by isolation from plants and expensive chemical synthesis of SA has increased the importance of developing strategies for SA synthesis. In our previous studies, glycerol was observed to be an effective carbon source for SA accumulation in E. coli DHPYAAS-T7, where the PTS operon (ptsHIcrr) and aroL and aroK genes were inactivated, and the tktA, glk, aroE, aroF (fbr) , and aroB genes were overexpressed. For further investigation of the effects of glycerol aerobic fermentation on SA accumulation in E. coli BL21(DE3), the glpD, glpK genes and tktA, glk, aroE, aroF (fbr) , aroB genes were overexpressed simultaneously. The results indicated that SA production was increased 5.6-fold, while the yield was increased 5.3-fold over that of parental strain in shake flasks. It is demonstrated that the aerobic fermentation of glycerol associated with glpD and glpK gene overexpression increased glycerol flux, resulting in higher SA accumulation in E. coli BL21(DE3)-P-DK.
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http://dx.doi.org/10.1007/s11274-014-1753-6DOI Listing
December 2014

A novel bi-functional DNA vaccine expressing VP1 protein and producing antisense RNA targeted to 5'UTR of foot-and-mouth disease virus can induce both rapid inhibitory effect and specific immune response in mice.

Authors:
Bin Yang Xi Lan Xueri Li Xiangping Yin Baoyu Li Xiaorong Han Yanmin Li Zhidong Zhang Jixing Liu

Vaccine 2008 Oct 9;26(43):5477-83. Epub 2008 Aug 9.

Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 11, Lanzhou, Gansu 730046, PR China.

To overcome the inability of a conventional vaccine against a virus to induce rapid protection against viral challenge, a novel strategy was performed to generate a novel bi-functional vector expressing antisense RNA targeted to 5' untranslated regions (UTR) and VP1 protein of foot-and-mouth disease virus (FMDV). FMDV 5'UTR containing the viral RNA replication start elements was inserted inversely into the pIRES vector to produce antisense RNA, followed by insertion of FMDV VP1 gene to generate a recombinant plasmid pAS-IR-VP1. BHK-21 cells transfected with pAS-IR-VP1 plasmid showed a specific resistance against FMDV infection. In mice vaccinated with this plasmid, T cell proliferation was significantly higher than that in an unvaccinated control group. Anti-FMDV antibodies were detected up to 1:64 in the serum collected from mice boosted with pAS-IR-VP1 at 21 days after the first immunization. At 6h post-vaccination 50-83% of the suckling mice survived a challenge with FMDV. The results demonstrated that a novel bi-functional DNA vaccine, producing antisense RNA targeted to FMDV 5'UTR and expressing VP1 protein, has been successfully constructed and was able to induce a rapid inhibitory effect and immune response against FMDV infection in mice.
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http://dx.doi.org/10.1016/j.vaccine.2008.07.060DOI Listing
October 2008
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