Publications by authors named "Xiaoqing Liu"

797 Publications

Defect Engineering Triggers Exceptional Sonodynamic Activity of Manganese Oxide Nanoparticles for Cancer Therapy.

ACS Appl Bio Mater 2022 Aug 11. Epub 2022 Aug 11.

Sun Yat-Sen University Cancer Center, State Key Lab oratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine. MOE of the Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou510275, PR China.

Sonodynamic therapy (SDT) has received increasing interest in cancer treatment, but its clinical application is still constrained by the low activity of sonosensitizers and their unclear mechanism. Herein, a kind of oxygen-deficient manganese oxide (MnO) nanoparticles with greatly enhanced sonodynamic activity and good biocompatibility is developed as an advanced sonosensitizer. The introduced oxygen defects can remarkably enhance the electrical conductivity of manganese oxide (MnO) nanoparticles and serve as charge trapping sites to prohibit the electron-hole pair recombination upon ultrasound (US) irradiation. Such distinct merits promote the generation of reactive oxygen species (ROS), making MnO as a decent sonosensitizer for SDT, and thus endowing MnO with higher ROS production under US irradiation. As a demonstration, the MnO nanoparticles decorated by 1,2-distearoyl--glycero-3-phosphoethanolamine--[methoxy(polyethylene glycol)-2000] (MnO-DSPE-PEG), a biocompatible coverage to enhance the dispersion ability, achieve a superior tumor killing efficiency of 96%, substantially higher than the MnO-DSPE-PEG counterpart (9%). Our experimental results also reveal that MnO-DSPE-PEG nanoparticles induce the death of tumor cells by targeting polyunsaturated fatty acids in their membrane with US-triggered ROS. Furthermore, the as-designed sonosensitizers exhibit negligible toxicity toward the treated mice.
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http://dx.doi.org/10.1021/acsabm.2c00445DOI Listing
August 2022

Metal Ions as the Third Component Coordinate with the Guest to Stereoscopically Enhance the Phosphorescence Properties of Doped Materials.

J Phys Chem Lett 2022 Aug 11:7607-7617. Epub 2022 Aug 11.

College of Chemistry and Materials Engineering, Wenzhou University, Wenzhou 325035, P.R. China.

The construction of multicomponent doped systems is an important direction for the development of phosphorescence materials. Herein, benzophenone is selected as the host, phenylquinoline isomers are designed as guests, and seven metal ions are selected as the third component (Al, Cu, Zn, Ga, Ag, Cd, and In) to construct the three-component doped system. Ag and Cd can considerably increase the emission intensity up to 38 times, and the highest phosphorescence quantum efficiency reaches 70%. Al, Ga, and In can prolong the emission wavelength, and the phosphorescence wavelength can be red-shifted up to 60 nm. Cu, Ga, and In can extend the phosphorescence lifetime by a maximum of 3.6 times. A series of experiments demonstrated that the coordination of metals and guests is the key to improve the phosphorescence properties. This work presents a simple and effective strategy to enhance the phosphorescence properties of doped materials.
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http://dx.doi.org/10.1021/acs.jpclett.2c02057DOI Listing
August 2022

Effectiveness and safety of low-dose interferon alpha-2a treatment in Behçet's Syndrome with refractory vascular or neurological involvement: a case series.

Ther Adv Chronic Dis 2022 26;13:20406223221111285. Epub 2022 Jul 26.

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng-qu, Beijing 100730, China.

Objective: The aim of this study was to evaluate the effectiveness and safety of low-dose interferon alpha-2a (IFNα2a) in Behçet's syndrome (BS) patients with refractory vascular/cardiac or neurological involvement.

Methods: In this retrospective cohort study, we consecutively included 25 BS patients with refractory vascular/cardiac ( = 16) or neurological involvement ( = 9) who received IFNα2a treatment in our center between June 2018 and September 2021. The low-dose IFNα2a (3 million IU, every other day) was used as an add-on treatment with the continuation of glucocorticoids (GCs) and immunosuppressants.

Results: In total, 25 patients (20 males, 5 females) with a mean age of 31.92 ± 9.25 years were included. IFNα2a was administered for BS patients with refractory vascular/cardiac involvement ( = 16) and neurological involvement ( = 9). Before the initiation of IFNα2a, patients had insufficient response or intolerance to conventional therapies. After a median follow-up of 23 [interquartile range (IQR), 11-30] months, all patients achieved clinical improvement. The Behçet's disease Current Activity Form (BDCAF) score improved significantly (5 0, median,  < 0.0001). BS Overall Damage Index (BODI) and vasculitis damage index (VDI) remain stable ( > 0.05). Decrease in erythrocyte sedimentation rate [ESR; 24 (IQR, 12-43.5) 5 (IQR, 2.75-10.5) mm/h,  = 0.0001] and C-reactive protein [CRP; 6.64 (IQR, 3.67-19.82) 1.24 (IQR, 0.24-3.12) mg/liter,  < 0.005] was achieved effectively. The median GCs dosage tapered from 26.25 (IQR, 11.88-41.25) to 10.00 (IQR, 7.50-10.63) mg/d,  < 0.0001. Immunosuppressants were also reduced in number ( < 0.005). No serious adverse events were observed during follow-up.

Conclusion: Our study suggests that low-dose IFNα2a, combined with GCs and immunosuppressants, is well-tolerated and effective for BS patients with refractory vascular/cardiac or neurological involvement and has a steroid- and immunosuppressant-sparing effect.
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http://dx.doi.org/10.1177/20406223221111285DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340357PMC
July 2022

Two-Wave Variable Nanotheranostic Agents for Dual-Mode Imaging-Guided Photo-Induced Triple-Therapy for Cancer.

Adv Sci (Weinh) 2022 Aug 2:e2201834. Epub 2022 Aug 2.

Department of Pharmaceutics, Key Laboratory of Chemical Biology (Ministry of Education), NMPA Key Laboratory for Technology Research and Evaluation of Drug Products, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Wenhua Xi Road, Jinan, Shandong Province, 250012, China.

Photothermal therapy (PTT) is a promising strategy for cancer treatment, but its clinical application relies heavily on accurate tumor positioning and effective combination. Nanotheranostics has shown superior application in precise tumor positioning and treatment, bringing potential opportunities for developing novel PTT-based therapies. Here, a nanotheranostic agent is proposed to enhance magnetic resonance imaging (MRI)/ near-infrared fluorescence imaging (NIRFI) imaging-guided photo-induced triple-therapy for cancer. Thermosensitive liposomes co-loaded with SPIONs/IR780 and Abemaciclib (SIA-TSLs), peptide ACKFRGD, and click group 2-cyano-6-amino-benzothiazole (CABT) are co-modified on the surface of SIA-TSLs to form SIA-αTSLs. ACKFRGD can be hydrolyzed to expose the 1, 2-thiolamino groups in the presence of cathepsin B in tumors, which click cycloaddition with the cyano group on CABT, resulting in the formation of SIA-αTSLs aggregates. The aggregation of SIA-αTSLs in tumors enhances the MRI/NIRFI imaging capability and enables precise PTT. Photo-induced triple-therapy enhances precision cancer therapy. First, PTT ablates specific tumors and induces ICD via localized photothermal. Second, local tumor heating promotes the rupture of SIA-αTSLs, which release Abemaciclib to block the tumor cell cycle and inhibit Tregs proliferation. Third, injecting GM-CSF into tumor tissue leads to recruitment of dendritic cells and initiation of antitumor immunity. Collectively, these results present a promising nanotheranostic strategy for future cancer therapy.
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http://dx.doi.org/10.1002/advs.202201834DOI Listing
August 2022

Patients with infectious diseases undergoing mechanical ventilation in the intensive care unit have better prognosis after receiving metagenomic next-generation sequencing assay.

Int J Infect Dis 2022 Jul 28. Epub 2022 Jul 28.

State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Department of Pulmonary and Critical Care Medicine, 151 Yanjiang Road, Guangzhou, 510120, China.. Electronic address:

Objectives: To evaluate the relation between mNGS and the prognosis of patients with infectious diseases undergoing mechanical ventilation in the intensive care unit (ICU).

Design: This is a single-center observational study, comparing non-randomly assigned diagnostic approaches. We analyzed the medical records of 228 patients with suspected infectious diseases undergoing mechanical ventilation in the ICU from March 2018 to May 2020. The concordance of pathogen results was also assessed for the results of mNGS, culture and PCR assays.

Results: The 28-day mortality of the patients in the mNGS group was lower after the baseline difference correction (19.23% (20/104) vs. 29.03% (36/124) , p=0.039). Subgroup analysis showed that mNGS assay associates with improved 28-day mortality of non-immunosuppressive patients (14.06% vs. 29.82%, p=0.018) . Not performing mNGS assay, higher APACHE II score and hypertension are independent risk factors for 28-day mortality. The mNGS assay presented advantage in pathogen positivity (69.8% double positive and 25.0% mNGS positive only), and the concordance between thest two assays were 79.0%.

Conclusions: mNGS survey may be associated with a better prognosis as the reduction of 28-day mortality of patients with infectious diseases on mechanical ventilation in ICU. This technique presented advantage in pathogen positivity than traditional methods.
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http://dx.doi.org/10.1016/j.ijid.2022.07.062DOI Listing
July 2022

Evaluation of the Brewing Characteristics, Digestion Profiles, and Neuroprotective Effects of Two Typical Se-Enriched Green Teas.

Foods 2022 Jul 21;11(14). Epub 2022 Jul 21.

Key Laboratory for Deep Processing of Major Grain and Oil, Ministry of Education, Hubei Key Laboratory for Processing and Transformation of Agricultural Products, Wuhan Polytechnic University, Wuhan 430023, China.

As a functional beverage, selenium (Se)-enriched green tea (Se-GT) has gained increasing popularity for its superior properties in promoting health. In this study, we compared the brewing characteristics, in vitro digestion profiles, and protective effects on neurotoxicity induced through the amyloid-beta (Aβ) peptide of two typical Se-GTs (Enshi Yulu (ESYL) and Ziyang Maojian (ZYMJ), representing the typical low-Se green tea and high-Se green tea, respectively). ESYL and ZYMJ showed similar chemical component leaching properties with the different brewing methods, and the optimized brewing conditions were 5 min, 90 °C, 50 mL/g, and first brewing. The antioxidant activities of the tea infusions had the strongest positive correlation with the tea polyphenols among all of the leaching substances. The tea infusions of ESYL and ZYMJ showed similar digestive behaviors, and the tea polyphenols in the tea infusions were almost totally degraded or transferred after 150 min of dynamic digestion. Studies conducted in a cell model of Alzheimer's disease (AD) showed that the extract from the high-Se green tea was more effective for neuroprotection compared with the low-Se green tea. Overall, our results revealed the best brewing conditions and digestion behaviors of Se-GT and the great potential of Se-GT or Se-enriched green extract (Se-GTE) to be used as promising AD-preventive beverages or food ingredients.
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http://dx.doi.org/10.3390/foods11142159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318317PMC
July 2022

The photochemical behaviors of microplastics through the lens of reactive oxygen species: Photolysis mechanisms and enhancing photo-transformation of pollutants.

Sci Total Environ 2022 Jul 21;846:157498. Epub 2022 Jul 21.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia. Electronic address:

The photoaging mechanisms of various polymers have been explored based on the basic autoxidation scheme (BAS) before 10 years ago, however current research verified some defects in the BAS in both thermodynamic and dynamics. These defects are troublesome because they are associated with the hydrogen abstraction which is central to continuously perform the photooxidation process of microplastics. These found indicated that we might wrongly inferred photo-oxidation process of some microplastics. In addition, the important role of reactive oxygen species (ROS) in the type-dependent photoaging process of various microplastics has been revealed recently. In this case, fully and accurately understanding the photoaging mechanisms of different microplastics in environment is a priority to further manage the ecological risk of microplastics. Herein, this review aims to revise and update the degradation process of microplastics based on the revised BAS and in the perspective of ROS. Specifically, the modification of BAS is firstly discussed. The photoaging mechanisms of representative microplastics (i.e., polyethylene, polystyrene and polyethylene terephthalate) are then updated based on the corrected BAS. Additionally, the role of ROS in their photolysis process and the possibility of microplastics as photosensitizers/mediators to regulate the fate of co-existent pollutants are also analyzed. Finally, several perspectives are then proposed to guide future research on the photoaging behaviors of microplastics. This review would pave the way for the understanding of microplastic photoaging and the management of plastic pollution in environments.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157498DOI Listing
July 2022

Comparison of Anticancer Activities and Biosafety Between Serovar Typhimurium ΔppGpp and VNP20009 in a Murine Cancer Model.

Front Microbiol 2022 29;13:914575. Epub 2022 Jun 29.

School of Biomedical Sciences, Hunan University, Changsha, China.

defective in guanosine 5'-diphosphate-3'-diphosphate (ppGpp) synthesis (ΔppGpp) is an attenuated strain with good biosafety and excellent anticancer efficacy. It has been widely applied in preclinical studies of anticancer therapy for various types of solid cancer. VNP20009 is another genetically modified auxotrophic strain with 108-kb deletion, , , and many single nucleotide polymorphisms (SNPs); it has shown promising therapeutic efficacy in various preclinical tumor models and entered phase I clinical trials. Here, the invasion activities and virulence of ΔppGpp were obviously lower than those of the VNP20009 strain when tested with cancer cells . In addition, the MC38 tumor-bearing mice showed comparable cancer suppression when treated with ΔppGpp or VNP20009 intravenously. However, the ΔppGpp-treated mice showed 16.7% of complete cancer eradication and prolonged survival in mice, whereas VNP20009 showed higher toxicity to animals, even with equal tumor size individually. Moreover, we found decreased levels of inflammatory cytokines in circulation but strengthened immune boost in tumor microenvironments of ΔppGpp-treated mice. Therefore, the engineered ΔppGpp has high potential for cancer therapeutics, and it is a promising option for future clinical cancer therapy.
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http://dx.doi.org/10.3389/fmicb.2022.914575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277105PMC
June 2022

Commensal microbe-derived SCFA alleviates atrial fibrillation via GPR43/NLRP3 signaling.

Int J Biol Sci 2022 27;18(10):4219-4232. Epub 2022 Jun 27.

Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

Dysbiotic gut microbiota (GM) and NLRP3 inflammasome are proarrhythmic factors in atrial fibrillation (AF). Herein, whether short-chain fatty acid (SCFA) produced from GM fermentation of dietary fiber serving as invisible mediators is yet unclear. Thus, the current study aimed to determine whether SCFA alleviated from NLRP3 signaling-mediated atrial remodeling protects AF development. First, a cross-sectional study based on the GC-MS metabolomics was performed to explore the association between fecal SCFA levels and AF traits in a cohort consisted of 48 individuals. Then, a well-established mice model fed diet deficient or enriched in dietary fiber was established to elucidate the pathophysiological role of SCFA involved in AF susceptibility, atrial remodeling, and G-protein-coupled receptor 43 (GPR43)/NLRP3 signaling. Finally, the effects of SCFA were verified on HL-1 cells. Fecal SCFA levels were remarkably reduced in AF patients with a declining trend from paroxysmal to persistent AF. Prolonged P wave duration based on surface ECG and increased left atrial diameter gained from echocardiography was identified in low-fiber diet mice but lost in SCFA-supplemented group. Lack of dietary fiber enhanced susceptibility to AF under burst pacing, whereas SCFA might exert a protective effect. The supplementation of SCFA prevented dietary fiber deficiency-upregulated phosphorylation of calmodulin-dependent protein kinase II and ryanodine receptor 2, the disarray fibrosis, collagen expression, and NLRP3 inflammasome activation in atrial tissue. Finally, the AF protective roles of SCFA were identified through GPR43 mediated deactivation of NLRP3 by GPR43 knockdown in HL-1 cells. SCFA derived from dietary fiber fermentation by gut commensals alleviates AF development via GPR43/NLRP3 signaling.
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http://dx.doi.org/10.7150/ijbs.70644DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274492PMC
July 2022

Multiply Guaranteed and Successively Amplified Activation of a Catalytic DNA Machine for Highly Efficient Intracellular Imaging of MicroRNA.

Small 2022 Aug 17;18(32):e2203341. Epub 2022 Jul 17.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.

DNA amplification machines show great promise for intracellular imaging, yet are always constrained by off-site machinery activation or signal leakage, originating from the inherent thermodynamically driven hybridization between machinery substrates. Herein, an entropy-driven catalytic DNA amplification machine is integrated with the on-site amplified substrate exposure procedure to realize the high-contrast in vivo imaging of microRNA (miRNA). The key machinery substrate (fuel strands) is initially split into substrate subunits that are respectively grafted into an auxiliary DNA polymerization amplification accessory for eliminating the undesired signal leakage. Meanwhile, in target cells, the auxiliary polymerization accessory can be motivated by cell-specific mRNA for successively restoring their intact machine-propelling functions for guaranteeing the on-site amplified imaging of miRNA with high specificity. This intelligent on-site multiply guaranteed machinery can improve the specificity of catalytic DNA machines for discriminating different cell types and, thus, can provide a remarkable prospect in biomedical diagnosis.
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http://dx.doi.org/10.1002/smll.202203341DOI Listing
August 2022

Emerging electrochemical techniques for identifying and removing micro/nanoplastics in urban waters.

Water Res 2022 Aug 10;221:118846. Epub 2022 Jul 10.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, NSW 2007, Australia. Electronic address:

The ubiquitous micro/nanoplastics (MPs/NPs) in urban waters are priority pollutants due to their toxic effects on living organisms. Currently, great efforts have been made to realize a plastic-free urban water system, and the identification and removal of MPs/NPs are two primary issues. Among diverse methods, emerging electrochemical techniques have gained growing interests owing to their facile implementation, high efficiency, eco-compatibility, onsite operation, etc. Herein, recent progress in the electrochemical identification and removal of MPs/NPs in urban waters are comprehensively reviewed. The electrochemical sensing of MPs/NPs and their released pollutants (e.g., bisphenol A (BPA)) has been analyzed, and the sensing principles and the featured electrochemical devices/electrodes are examined. Afterwards, recent applications of electrochemical methods (i.e., electrocoagulation, electroadsorption, electrokinetic separation and electrochemical degradation) in MPs/NPs removal are discussed in detail. The influences of critical parameters (e.g., plastics' property, current density and electrolyte) in the electrochemical identification and removal of MPs/NPs are also analyzed. Finally, the current challenges and prospects in electrochemical sensing and removal of MPs/NPs in urban waters are elaborated. This review would advance efficient electrochemical technologies for future MPs/NPs pollutions management in urban waters.
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http://dx.doi.org/10.1016/j.watres.2022.118846DOI Listing
August 2022

Removal of microplastics and nanoplastics from urban waters: Separation and degradation.

Water Res 2022 Aug 4;221:118820. Epub 2022 Jul 4.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, NSW 2007, Australia. Electronic address:

The omnipresent micro/nanoplastics (MPs/NPs) in urban waters arouse great public concern. To build a MP/NP-free urban water system, enormous efforts have been made to meet this goal via separating and degrading MPs/NPs in urban waters. Herein, we comprehensively review the recent developments in the separation and degradation of MPs/NPs in urban waters. Efficient MP/NP separation techniques, such as adsorption, coagulation/flocculation, flotation, filtration, and magnetic separation are first summarized. The influence of functional materials/reagents, properties of MPs/NPs, and aquatic chemistry on the separation efficiency is analyzed. Then, MP/NP degradation methods, including electrochemical degradation, advanced oxidation processes (AOPs), photodegradation, photocatalytic degradation, and biological degradation are detailed. Also, the effects of critical functional materials/organisms and operational parameters on degradation performance are discussed. At last, the current challenges and prospects in the separation, degradation, and further upcycling of MPs/NPs in urban waters are outlined. This review will potentially guide the development of next-generation technologies for MP/NP pollution control in urban waters.
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http://dx.doi.org/10.1016/j.watres.2022.118820DOI Listing
August 2022

Development and validation of a clinical risk model to predict the hospital mortality in ventilated patients with acute respiratory distress syndrome: a population-based study.

BMC Pulm Med 2022 Jul 11;22(1):268. Epub 2022 Jul 11.

Department of Critical Care Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

Background: Large variability in mortality exists in patients of acute respiratory distress syndrome (ARDS), especially those with invasive ventilation. The aim of this study was to develop a model to predict risk of in-hospital death in ventilated ARDS patients.

Methods: Ventilated patients with ARDS from two public databases (MIMIC-III and eICU-CRD) were randomly divided as training cohort and internal validation cohort. Least absolute shrinkage and selection operator (LASSO) and then Logistic regression was used to construct a predictive model with demographic, clinical, laboratory, comorbidities and ventilation variables ascertained at first 24 h of ICU admission and invasive ventilation. Our model was externally validated using data from another database (MIMIC-IV).

Results: A total of 1075 adult patients from MIMIC-III and eICU were randomly divided into training cohort (70%, n = 752) and internal validation cohort (30%, n = 323). 521 patients were included from MIMIC-IV. From 176 potential predictors, 9 independent predictive factors were included in the final model. Five variables were ascertained within the first 24 h of ICU admission, including age (OR, 1.02; 95% CI: 1.01-1.03), mean of respiratory rate (OR, 1.04; 95% CI: 1.01-1.08), the maximum of INR (OR, 1.14; 95% CI: 1.03-1.31) and alveolo-arterial oxygen difference (OR, 1.002; 95% CI: 1.001-1.003) and the minimum of RDW (OR, 1.17; 95% CI: 1.09-1.27). And four variables were collected within the first 24 h of invasive ventilation: mean of temperature (OR, 0.70; 95% CI: 0.57-0.86), the maximum of lactate (OR, 1.15; 95% CI: 1.09-1.22), the minimum of blood urea nitrogen (OR, 1.02; 95% CI: 1.01-1.03) and white blood cell counts (OR, 1.03; 95% CI: 1.01-1.06). Our model achieved good discrimination (AUC: 0.77, 95% CI: 0.73-0.80) in training cohort but the performance declined in internal (AUC: 0.75, 95% CI: 0.69-0.80) and external validation cohort (0.70, 95% CI: 0.65-0.74) and showed modest calibration.

Conclusions: A risk score based on routinely collected variables at the start of admission to ICU and invasive ventilation can predict mortality of ventilated ARDS patients, with a moderate performance.
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http://dx.doi.org/10.1186/s12890-022-02057-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277886PMC
July 2022

Three-Bit Digital Comparator Based on Intracell Diffusion of Silver Single Atom.

Nano Lett 2022 07 11;22(14):5909-5915. Epub 2022 Jul 11.

Department of Physics, Nanchang University, Nanchang 330031, China.

Using a single atom to construct electronic components is a promising route for the microminiaturization of electronic instruments. However, effective control of the intrinsic property in a molecular/atomic prototype component is full of challenges. Here, we present that the intracell diffusion behavior of a target Ag single atom within a unit cell of Si reconstruction is controllably modulated by constructing Ag nanoclusters and arrays in the neighboring cells. Moreover, a three-bit digital comparator device is fabricated on the basis of the diffusion time of a Ag single atom that can be effectively regulated by using the intercoupling between the target Ag monomer and the surrounding metal arrays.
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http://dx.doi.org/10.1021/acs.nanolett.2c01916DOI Listing
July 2022

Suppressing the activity of down-regulates the expression of renal fibrosis related genes in primary glomerular cells.

Transl Pediatr 2022 Jun;11(6):882-890

Department of Nephrology, Jiangxi Provincial Children's Hospital, Nanchang, China.

Background: C-X-C chemokine receptor type 4 () has a certain effect on renal fibrosis, and there are few specific studies in cells. We want to investigate the impact of suppressing activity on the expression of renal fibrosis-related genes in primary glomerular endothelial cells, mesangial cells, and podocytes.

Methods: Immunofluorescence assays were used to determine the purity of isolated glomerular endothelial cells, mesangial cells, and podocytes. knockdown cell lines were established by transfecting the short hairpin (sh)RNA against CXCR4. T140 and AMD3100 were used to inhibit the activity of . LY294002 was used to inhibit the activity of phosphoinositide 3-kinase (). The mRNA expression of was determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). The protein expression of , , matrix metallopeptidase , and vascular cell adhesion protein 1 () was evaluated by Western blot analysis.

Results: High purity was observed on isolated primary glomerular endothelial cells and podocytes. However, the purity of isolated mesangial cells was relatively low. The mRNA expression of was significantly suppressed by the transfection of shRNA. Compared to control cells, the expression of , collagen , and were dramatically downregulated in cell lines transfected with shRNA against . Furthermore, cell lines treated with T140, AMD3100, or LY294002 also showed downregulated expression of these proteins compared to untreated cells. No significant differences were observed in the protein expression of these proteins between control cells and cells transfected with the shRNA negative control (NC).

Conclusions: Suppressing the activity of downregulated the expression of renal fibrosis-related genes in primary glomerular cells, even under a non-inflammatory state.
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http://dx.doi.org/10.21037/tp-22-157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253943PMC
June 2022

Wettability Controlled Surface for Energy Conversion.

Small 2022 Aug 6;18(31):e2202906. Epub 2022 Jul 6.

Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, 1219 Zhongguan West Road, Zhenhai District, Ningbo, 315201, P. R. China.

To achieve clean and high-efficiency utilization of renewable energy, functional surfaces with controllable and patternable wettability are becoming a fast-growing research focus. In this work, a laser scribing strategy to fabricate patterned graphene surfaces that are capable of energy conversion in different forms is demonstrated. Using the laser raster-scanning and vector-scanning modes, two distinct surface structures are constructed on polybenzoxazine substrate, yielding a superhydrophilic (LSHL) surface and superhydrophobic (LSHB) surface, respectively. Of particular note is that the unique hierarchical structure of LSHB surface has endowed it with quite a robust superwetting behaviors. Further profiting from the flexibility of the processing method, wettability patterns with spatially resolved LSHL and LSHB regions are designed, achieving the conversion of surface energy to liquid kinetic energy. This also offers a tractable approach to fabricate wettability-engineered devices that enable the directional, pumpless transport of water by capillary pressure gradient and the selective surface cooling via jet impingement. In addition, the LSHB surface demonstrates the high conversion of electric-to-thermal energy (222 °C cm W ) and light-to-thermal energy (88%). Overall, the material system and processing method present a promising step forward to developing easy-fabricated graphene surfaces with spatially controlled wettability for efficient energy utilization and conversion.
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http://dx.doi.org/10.1002/smll.202202906DOI Listing
August 2022

Co-expression of transcription factors ZmC1 and ZmR2 establishes an efficient and accurate haploid embryo identification system in maize.

Plant J 2022 Jul 6. Epub 2022 Jul 6.

National Maize Improvement Center of China, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.

Because of their high efficiency during chromosome doubling, immature haploid maize (Zea mays L.) embryos are useful for doubled haploid production. The R1-nj marker is commonly used in doubled haploid breeding and has improved the efficiency of haploid identification. However, its effectiveness is limited by genetic background and environmental factors. We addressed this technical challenge by developing an efficient and accurate haploid embryo identification marker through co-expression of two transcription factor genes (ZmC1 and ZmR2) driven by the embryo-aleurone-specific bidirectional promoter P ; these factors can activate anthocyanin biosynthesis in the embryo and aleurone layer during early seed development. We developed a new haploid inducer, Maize Anthocyanin Gene InduCer 1 (MAGIC1), by introducing the transgenes into the haploid inducer line CAU6. MAGIC1 could identify haploids at 12 days after pollination, which is nine days earlier than CAU6. Importantly, MAGIC1 increased haploid identification accuracy to 99.1%, compared with 88.3% for CAU6. In addition, MAGIC1 could effectively overcome the inhibition of anthocyanin synthesis in some germplasms. Furthermore, an upgraded anthocyanin marker was developed from ZmC1 and ZmR2 to generate MAGIC2, which could identify haploids from diploids due to differential anthocyanin accumulation in immature embryos, coleoptiles, sheaths, roots, leaves, and dry seeds. This haploid identification system is more efficient and accurate than the conventional R1-nj-based method, and it simplifies the haploid identification process. Therefore, this system provides technical support for large-scale doubled haploid line production.
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http://dx.doi.org/10.1111/tpj.15888DOI Listing
July 2022

Improved Reversible Zinc Storage Achieved in a Constitutionally Crystalline-Stable Mn(VO ) Nanobelts Cathode.

Chemistry 2022 Jul 5:e202201687. Epub 2022 Jul 5.

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, P. R. China.

Rechargeable zinc-ion batteries (ZIBs) are potential for grid-scale applications owing to their safety, low price, and available sources. The development of ZIBs cathode with high specific capacity, wide operating voltage window and stable cyclability is urgently needed in next-generation commercial batteries. Herein, we report a structurally crystalline-stable Mn(VO ) nanobelts cathode for ZIBs prepared via a facile hydrothermal method. The as-synthesized Mn(VO ) exhibited high specific capacity of 350 mAh g at 0.1 A g , and maintained a capacity retention of 92 % after 10,000 cycles at 2 A g . It also showed good rate performance and obtained a reversible capacity of up to 200 mAh g after 600 cycles at 0.2 A g under -20 °C. The electrochemical tests suggest that Mn(VO ) nanobelts impart fast Zn ions migration, and the introduction of manganese atoms help make the structures more indestructible, leading to a good rate performance and prolonged cycle lifespan.
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http://dx.doi.org/10.1002/chem.202201687DOI Listing
July 2022

An Autocatalytic DNA Circuit Based on Hybridization Chain Assembly for Intracellular Imaging of Polynucleotide Kinase.

ACS Appl Mater Interfaces 2022 Jul 5;14(28):31727-31736. Epub 2022 Jul 5.

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.

Polynucleotide kinase (PNK) plays an essential role in various cellular events by regulating phosphorylation processes, and abnormal homeostasis of PNK could cause many human diseases. Herein, we proposed an autocatalytic hybridization system (AHS) through the elaborate integration of hybridization chain assembly (HCA) and catalytic DNA assembly (CDA) that enables a highly efficient positive feedback amplification. The PNK-targeting AHS biosensor is composed of three modules: a recognition module, an HCA amplification module, and a CDA autocatalytic module. In the presence of PNK, the recognition module could transform the PNK input into an exposed nucleic acid initiator (). Then the initiator strand could trigger the autonomous HCA process in the amplification module, and the resulted HCA products could reassemble the split CDA trigger strand , subsequently inducing the CDA process in the autocatalytic module to form abundant DNA duplex products. Consequently, the embedded initiator strand was liberated from the CDA duplex product to autonomously trigger the new rounds of HCA circuit. The rational integration and cooperative cross-activation between the HCA and CDA module could prominently accelerate the reaction and realize the exponential amplification efficiency by initiator regeneration. As a result, the self-sustainable AHS amplifier could implement the sensitive detection of PNK and in biological samples and further fulfill accurate monitoring of the intracellular PNK activity and the effective screening of PNK inhibitors. This work paves a way for exploiting highly efficient artificial DNA circuits to analyze low-abundance biomarkers, holding great potential in biochemical research and clinical diagnosis.
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http://dx.doi.org/10.1021/acsami.2c08523DOI Listing
July 2022

Stable and efficient luminescence of CsPbBrIx PQDs glass: a potential material for wide color gamut display.

Opt Lett 2022 Jul;47(13):3335-3338

Zero-dimensional CsPbBrIx perovskite quantum dots (PQDs) glass is successfully prepared via a melt quenching method, which provides infinite possibilities for achieving the whole family of zero-dimensional PQDs glass. The test results demonstrate excellent thermal stability and high photoluminescence quantum yields (PLQYs) of CsPbBrIx PQDs glass (up to 50%). Finally, the combination of CsPbBrIx PQDs glass with an InGaN blue chip is used to fabricate white light-emitting diodes (WLED), which show good color stability at a large operating current, and the color gamut area reaches 137% of NTSC. The above results indicate that zero-dimensional CsPbBrIx PQDs glass materials have a broad application prospect in the display lighting field.
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http://dx.doi.org/10.1364/OL.465445DOI Listing
July 2022

On-Site Nonenzymatic Orthogonal Activation of a Catalytic DNA Circuit for Self-Reinforced In Vivo MicroRNA Imaging.

Angew Chem Int Ed Engl 2022 Jul 1. Epub 2022 Jul 1.

Wuhan University, College of Chemistry and Molecular Sciences, Bayi Road 299, 430072, Wuhan, CHINA.

The wide extracellular-intracellular distribution of microRNA requires the on-site, robust and efficient activation of catalytic DNA circuits inside live cells. Herein, we developed an efficient non-enzymatic circuitry activation strategy to realize the orthogonally controlled catalytic DNA (CCD) circuit for achieving the high-fidelity in vivo microRNA imaging through the multiply guaranteed molecular recognition and progressively accelerated signal amplification. For predictable on-site activation and competent catalytic efficiency, the dominating circuitry fuel strand was initially splitted into inactive fuel subunits that were grafted into an auxiliary catalytic circuit, where the in-cell-specific mRNA triggered the orthogonal amplification of active fuel strands for sensitive target detection through the chief entropy-driven catalytic DNA circuit. We believe that the on-site orthogonal circuitry activation methodology can ingeniously contribute to clinical diagnosis and prognosis.
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http://dx.doi.org/10.1002/anie.202206529DOI Listing
July 2022

Comparison of diagnostic accuracy of QuantiFERON-TB Gold Plus and T-SPOT.TB in the diagnosis of active tuberculosis in febrile patients.

J Evid Based Med 2022 Jun 28;15(2):97-105. Epub 2022 Jun 28.

Division of Infectious Diseases, Department of Internal medicine, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Objective: This study aimed to compare the accuracy of QuantiFERON-TB Gold Plus (QFT-Plus) and T-SPOT.TB for diagnosing active tuberculosis (ATB) in febrile patients, to explore influencing factors of positive results and to verify the potential value of QFT-Plus in the identification of ATB and latent tuberculosis infection (LTBI).

Methods: A total of 240 febrile patients with ATB (n = 80) and non-ATB (n = 160) were recruited to assess the accuracy of QFT-Plus and T-SPOT.TB for diagnosing ATB. Multivariable logistic regression was used to analyze the influencing factors of positive results.

Results: The proportion of indeterminate results (ITRS) in QFT-Plus and T-SPOT.TB were 3.3% and 0%, respectively. The consistency between the results of the QFT-Plus and T-SPOT.TB was substantial. The area under the receiver operating characteristic curve (AUROC) of the QFT-Plus and T-SPOT.TB for diagnosing ATB was 0.792 and 0.849 (p = 0.070), respectively. The sensitivity of differentiating ATB from non-ATB was 92.2% in QFT-Plus versus 95.0% in T-SPOT.TB. The influencing factors of T-SPOT.TB positive result were male (odds ratio (OR) = 2.33, 95% confidence interval (CI) 1.27-4.26, p = 0.006), evidence of previous TB (OR 11.36, 95% CI 4.62-27.94, p < 0.001), while male (OR = 3.17, 95% CI 1.73-5.84, p < 0.001), evidence of previous TB (OR = 7.58, 95% CI 3.60-15.98, p <0.001), and use of immunosuppressant (OR = 0.49, 95% CI 0.260.94, p = 0.030) were influencing factors for QFT-Plus positive result. There was no significant difference in QFT-Plus in differentiating ATB from LTBI in febrile patients.

Conclusion: There was no significant difference between QFT-Plus and T-SPOT.TB for diagnosing ATB in febrile patients. QFT-Plus is prone to ITRS. The influencing factors including males, evidence of the previous TB, and use of immunosuppressant should be considered when interpreting positive results.
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http://dx.doi.org/10.1111/jebm.12477DOI Listing
June 2022

Phylogenetic signature and prevalence of natural resistance-associated substitutions for hepatitis C virus genotypes 3a and 3b in southwestern China.

J Virus Erad 2022 Jun 15;8(2):100071. Epub 2022 Jun 15.

Department of Infectious Diseases, Institute for Viral Hepatitis, The Key Laboratory of Molecular Biology for Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Patients infected with hepatitis C (HCV) genotype (GT) 3, especially GT3b, are still difficult to cure. GT3b is more common than GT3a in southwestern China. Here we aimed to investigate the prevalence of naturally occurring RASs in HCV GT3 in southwestern China and performed phylogenetic analysis.

Methods: Serum samples were collected from patients with HCV GT3 infection. Sanger sequencing was used to validate resistance-associated substitutions (RASs). Phylogenetic analysis was performed using MEGA X and the observed-minus-expected-squared algorithm was used to analyze amino acid covariance.

Results: A total of 136 patients were enrolled, including 41 HCV GT3a and 95 GT3b infected patients. In the NS5A region, the proportion of RASs found in GT3b (99%) was notably higher than in GT3a (9%). In the NS3 region, RASs prevalence in GT3b (5%) was lower than in GT3a (24%). NS5B-specific RASs were rare. Both the NS5A30k and L31 M substitutions occurred in 96% of GT3b sequences. The A30K + L31M combination was found in 94% of GT3b isolates, however, there were no A30K or L31M mutations observed in the GT3a sequence.

Conclusions: Significant differences were observed between HCV GT3a and GT3b in terms of RAS prevalence. The origin of GT3a appears to be more diverse compared with GT3b in southern China. Studies specifically aimed at HCV GT3b infection should be initiated to gain more insight into this subtype.
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http://dx.doi.org/10.1016/j.jve.2022.100071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218835PMC
June 2022

Methods for Rational Design of Advanced Zn-Based Batteries.

Small Methods 2022 Jun 23:e2200560. Epub 2022 Jun 23.

MOE of the Key Laboratory of Bioinorganic and Synthetic Chemistry, The Key Lab of Low-carbon Chem & Energy Conservation of Guangdong Province, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, P. R. China.

Rechargeable aqueous zinc-based batteries (AZBs) have received massive attention as promising contenders for the future large-scale energy storage due to their low cost, inherent safety, and abundant resources. However, the insufficient energy density and poor stability have become the key to hinder their further application. As is well known, the energy densities (E, Wh kg ) of AZBs are determined by the specific capacity (mAh g ) and output voltage (V). Given the fixed redox potential and capacity of the Zn metal anode, the energy density of AZBs is mainly determined by the cathode material, and the rich material systems of the cathode provide more possibilities to this field. Meanwhile, the methods to improve the stability and performance of the Zn anodes have gained more and more attention due to the severe Zn dendrite growth that can pierce the separator and lead to short-circuiting of the cell. Therefore, in this review, we comprehensively summarize the rational design methods in optimizing the cathode, anode, and device architecture, and classic examples of each catalogue are discussed in details as well. Last, the issues and outlook for further development of high performance AZBs are also presented.
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http://dx.doi.org/10.1002/smtd.202200560DOI Listing
June 2022

Localized Myocardial Anti-Inflammatory Effects of Temperature-Sensitive Budesonide Nanoparticles during Radiofrequency Catheter Ablation.

Research (Wash D C) 2022 31;2022:9816234. Epub 2022 May 31.

Beijing Key Laboratory of Micro-Nano Energy and Sensor, Beijing Institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 101400, China.

Radiofrequency (RF) catheter ablation has emerged as an effective alternative for the treatment of atrial fibrillation (AF), but ablation lesions will result in swelling and hematoma of local surrounding tissue, triggering inflammatory cell infiltration and increased release of inflammatory cytokines. Some studies have shown that the inflammatory response may be related to the early occurrence of AF. The most direct way to inhibit perioperative inflammation is to use anti-inflammatory drugs such as glucocorticoids. Here, we prepared polylactic-co-glycolic acid (PLGA) nanoparticles loaded with budesonide (BUD) and delivered them through irrigation of saline during the onset of ablation. Local high temperature promoted local rupture of PLGA nanoparticles, releasing BUD, and produced a timely and effective local myocardial anti-inflammatory effect, resulting in the reduction of acute hematoma and inflammatory cell infiltration and the enhancement of ablation effect. Nanoparticles would also infiltrate into the local myocardium and gradually release BUD ingredients to produce a continuous anti-inflammatory effect in the next few days. This resulted in a decrease in the level of inflammatory cytokine IL-6 and an increase of anti-inflammatory cytokine IL-10. This study explored an extraordinary drug delivery strategy to reduce ablation-related inflammation, which may prevent early recurrence of AF.
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http://dx.doi.org/10.34133/2022/9816234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178488PMC
May 2022

Graphene-Enabled Electric-Field Regulation and Ionic Redistribution Around Lithiophilic Aurum Nanoparticles Toward a Dendrite-Free and 2000-Cycle-Life Lithium Metal Battery.

Chemistry 2022 Jun 13:e202201151. Epub 2022 Jun 13.

Guangdong Provincial Key Laboratory of Plant Resources Biorefinery, School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, P. R. China.

Lithium metal batteries (LMBs) have attracted extensive attention owing to their high energy density. However, the uncontrolled volume changes and serious dendrite growth of the Li metal anode have hindered their commercialization. Herein, a three-dimensional Cu foam decorated with Au nanoparticles and conformal graphene layer was designed to tune the Li plating/stripping behaviors. The 3D-Cu conductive host anchored by lithiophilic Au nanoparticles can effectively alleviate the volume expansion caused by the continuous plating/stripping of Li and reduce the nucleation energy barrier. Notably, the conductive graphene not only facilitates the transfer of electrons, but also acts as an ionic rectifier, thereby avoiding the aggregation of local current density and Li ions around Au nanoparticles and enabling the uniform Li flux. As a result, the [email protected]/Li anode ensures the non-dendritic and homogeneous Li plating/stripping. Electrochemical results show that the symmetric [email protected]/Li cell delivers a low voltage hysteresis of 110 mV after 1000 h at 1 mA cm . Matched with a layered LiNi Co Mn O cathode, the NCM622||[email protected]/Li full cell exhibits a long cycle life of 2000 cycles and an ultra-low capacity decay rate (0.01 % per cycle).
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http://dx.doi.org/10.1002/chem.202201151DOI Listing
June 2022

Causal associations of alcohol consumption with cardiovascular diseases and all-cause mortality among Chinese.

Am J Clin Nutr 2022 Jun 10. Epub 2022 Jun 10.

Key Laboratory of Cardiovascular Epidemiology & Department of Epidemiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.

Background: The causal effects of moderate alcohol consumption on cardiovascular diseases (CVDs) are continuously debated, especially on coronary heart disease (CHD).

Objectives: We aimed to explore the causal associations of alcohol consumption with CVDs and all-cause mortality among Chinese males.

Methods: A prospective cohort study was conducted in 40,386 Chinese males, with 17,676 being genotyped for the rs671 variant in the aldehyde dehydrogenase 2 (ALDH2) gene. Cox proportional hazards model was conducted to estimate the effects of self-reported alcohol consumption. Mendelian randomization (MR) analysis was performed to explore the causality using rs671 as an instrumental variable.

Results: During the follow-up of 303,353 person-years, 2,406 incident CVD and 3,195 all-cause mortality were identified. J-shaped associations of self-reported alcohol consumption with incident CVD and all-cause mortality were observed, showing decreased risks for light (≤25 g/day) and moderate drinkers (25-≤60 g/day). However, MR analyses revealed a linear association of genetically predicted alcohol consumption with the incident CVD (Ptrend = 0.02), including both CHD (Ptrend = 0.03) and stroke (Ptrend = 0.02). The HRs (95% CIs) for incident CVD across increasing tertiles of genetically predicted alcohol consumption were 1 (reference), 1.18 (1.01, 1.38), and 1.22 (1.03, 1.46). After excluding heavy drinkers, the risk of incident CVD and all-cause mortality was increased by 27% and 20% per standard drink increment of genetically predicted alcohol consumption, respectively.

Conclusions: Our analyses extend the evidence of the harmful effect of alcohol consumption to total CVD (including CHD) and all-cause mortality, highlighting the potential health benefits of lowering alcohol consumption, even among light-to-moderate male drinkers.
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http://dx.doi.org/10.1093/ajcn/nqac159DOI Listing
June 2022

Boosting enzymatic degradation of cellulose using a fungal expansin: Structural insight into the pretreatment mechanism.

Bioresour Technol 2022 Aug 6;358:127434. Epub 2022 Jun 6.

State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

The recalcitrance of cellulosic biomass greatly hinders its enzymatic degradation. Expansins induce cell wall loosening and promote efficient cellulose utilization; however, the molecular mechanism underlying their action is not well understood. In this study, TlEXLX1, a fungal expansin from Talaromyces leycettanus JCM12802, was characterized in terms of phylogeny, synergy, structure, and mechanism of action. TlEXLX1 displayed varying degrees of synergism with commercial cellulase in the pretreatment of corn straw and filter paper. TlEXLX1 binds to cellulose via domain 2, mediated by CH-π interactions with residues Tyr291, Trp292, and Tyr327. Residues Asp237, Glu238, and Asp248 in domain 1 form hydrogen bonds with glucose units and break the inherent hydrogen bonding within the cellulose matrix. This study identified the expansin amino acid residues crucial for cellulose binding, and elucidated the structure and function of expansins in cell wall networks; this has potential applications in biomass utilization.
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http://dx.doi.org/10.1016/j.biortech.2022.127434DOI Listing
August 2022

T cell epitopes are largely conserved in the SARS-CoV-2 Omicron subvariant (BA.1, BA.2, BA.3, and GKA).

J Med Virol 2022 Jun 8. Epub 2022 Jun 8.

Department of Infectious Diseases, Institute for Viral Hepatitis, The Key Laboratory of Molecular Biology for Infectious Diseases, Chinese Ministry of Education, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

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http://dx.doi.org/10.1002/jmv.27925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348460PMC
June 2022

Association between blood ethylene oxide levels and the prevalence of hypertension.

Environ Sci Pollut Res Int 2022 Jun 7. Epub 2022 Jun 7.

Heart Center & Beijing Key Laboratory of Hypertension Research, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing, 100020, China.

The relationship of blood ethylene oxide levels with hypertension and blood pressure has not been addressed. A total of 5005 participants from the National Health and Nutrition Examination Survey (NHANES) 2013-2016 were enrolled. Hypertension was defined as a mean systolic blood pressure (SBP) of at least 140 mmHg, a mean diastolic blood pressure (DBP) of at least 90 mmHg, or both, and/or the self-reported use of prescription drugs for diagnosed hypertension. Generalized linear regression models and restricted cubic spline plots were performed to explore the associations of ethylene oxide levels with hypertension and blood pressure. The prevalence of hypertension in the study sample was 27.6%. After adjusting for confounding factors, compared with the lowest quantile, the odds ratios (ORs) with 95% confidence intervals (CIs) of hypertension across the quantiles of ethylene oxide levels were 0.80 (0.63, 1.03), 0.91 (0.71, 1.16), and 1.39 (1.06, 1.82), respectively (P-value for trend = 0.001). Compared with the lowest quantile, the highest quantile of blood ethylene oxide levels was significantly associated with the worst DBP profile by approximately 2.67 mmHg. Blood ethylene oxide levels showed a strong nonlinear and positive association with DBP, while no significant association was observed between blood ethylene oxide levels and SBP. These results provide epidemiological evidence of elevated blood levels of ethylene oxide in relation to a higher prevalence of hypertension and higher DBP. Further study is warranted to address these issues.
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http://dx.doi.org/10.1007/s11356-022-21130-zDOI Listing
June 2022
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