Publications by authors named "Xiaoqing Li"

393 Publications

Dasatinib enhances anti-leukemia efficacy of chimeric antigen receptor T cells by inhibiting cell differentiation and exhaustion.

J Hematol Oncol 2021 Jul 21;14(1):113. Epub 2021 Jul 21.

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou, China.

Relapses of CD19-expressing leukemia in patients who achieved initial remission after CART cell treatment have been reported to correlate with poor CART cells persistence. Sustained tonic signaling or strong activation drives CART cell differentiation and exhaustion, which limit the therapeutic efficacy and persistence of CART cells. Here, we identified dasatinib as the optimal candidate to prevent or reverse both CD28/CART and 4-1BB/CART cell differentiation and exhaustion during ex vivo expansion, which profoundly enhanced the therapeutic efficacy and in vivo persistence. Moreover, strong activation-induced CART cells differentiation, exhaustion and apoptosis driven by CD3/CD28 stimulation or antigen exposure were dramatically prevented or reversed by dasatinib treatment. Mechanistically, dasatinib markedly reduced the phosphorylation of Src and Lck, and downregulated the expression of genes involved in CAR signaling pathways, which resulted in the optimization of cell differentiation, exhaustion and apoptosis-related gene expression. Our study proposes a promising pharmacological approach for optimizing CART cells manufacture, and provides an experimental basis for reinvigorating CART cells in clinical application.
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http://dx.doi.org/10.1186/s13045-021-01117-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293573PMC
July 2021

Treatment of Wound Healing with Sequential Therapy to Accelerate Recovery and Inhibit Scar Hyperplasia: A Case Report.

Clin Cosmet Investig Dermatol 2021 6;14:821-825. Epub 2021 Jul 6.

Cosmetics Safety and Efficacy Evaluation Centre, Sichuan University West China Hospital, Chengdu, 610041, People's Republic of China.

Study Design/patients And Methods: A 19-year-old male was reported to have a postoperative facial trauma suture as a result of being involved in a car accident. Red light-emitting diode (LED) therapy (20 min, 96 J/cm, 633 nm), Botulinum Toxin Type A 36 IU injection, BroadBand Light and Er:YAG laser at various stages of wound healing were applied as the sequential therapy.

Results: Since the correction was promptly apparent and acceptable, the treatment proved secure and efficacious for repairing wound healing.

Conclusion: Clinically sequential therapy has demonstrated marked improvement in our case. Scar sequential therapy may offer a new strategy for wound healing recovery.
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http://dx.doi.org/10.2147/CCID.S319558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275119PMC
July 2021

Contextual prediction modulates musical tension: Evidence from behavioral and neural responses.

Brain Cogn 2021 Jul 1;152:105771. Epub 2021 Jul 1.

CAS Key Laboratory of Behavioral Science, Institute of Psychology, Chinese Academy of Sciences, Beijing, China; Department of Psychology, University of Chinese Academy of Sciences, Beijing, China. Electronic address:

Tension is a bridge between music structure and emotion. It is known that tension is affected by prediction in music listening as music unfolds. Combining behavioral and neural responses, the current research investigated how musical predictions influence tension in the process of prediction build-up based on musical context (anticipatory stage) and its integration with upcoming stimuli (integration stage). The results showed that, at the anticipatory stage, compared with high-prediction conditions, in low-prediction conditions tension curve changed faster and unstable, and a larger N5 in ERP response was elicited. Furthermore, at the integration stage, compared with congruent conditions, in incongruent conditions the behavioral rating of tension were higher regardless of the predictability of the final chord; a right negativity and P600 were elicited, and the amplitude of P600 was modulated by the predictability of the final chord. These results indicated that the effect of prediction on tension was modulated by contextual predictability. The findings provide a more comprehensive view on how musical prediction affects musical tension.
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http://dx.doi.org/10.1016/j.bandc.2021.105771DOI Listing
July 2021

Carbon budgeting belowground.

New Phytol 2021 Jul 3. Epub 2021 Jul 3.

CSIRO Agriculture & Food, St Lucia, Brisbane, QLD, 4067, Australia.

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http://dx.doi.org/10.1111/nph.17520DOI Listing
July 2021

Cannabinoid Receptors in Myocardial Injury: A Brother Born to Rival.

Int J Mol Sci 2021 Jun 26;22(13). Epub 2021 Jun 26.

Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

Cannabinoid receptors typically include type 1 (CB1) and type 2 (CB2), and they have attracted extensive attention in the central nervous system (CNS) and immune system. Due to more in-depth studies in recent years, it has been found that the typical CB1 and CB2 receptors confer functional importance far beyond the CNS and immune system. In particular, many works have reported the critical involvement of the CB1 and CB2 receptors in myocardial injuries. Both pharmacological and genetic approaches have been used for studying CB1 and CB2 functions in these studies, revealing that the brother receptors have many basic differences and sometimes antagonistic functions in a variety of myocardial injuries, despite some sequence or location identity they share. Herein, we introduce the general differences of CB1 and CB2 cannabinoid receptors, and summarize the functional rivalries between the two brother receptors in the setting of myocardial injuries. We point out the importance of individual receptor-based modulation, instead of dual receptor modulators, when treating myocardial injuries.
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http://dx.doi.org/10.3390/ijms22136886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268439PMC
June 2021

Association of Obesity and Body Fat Percentage with Pubertal State in Six- to Nine-Year-Old Chinese Females.

Child Obes 2021 Jun 29. Epub 2021 Jun 29.

Department of Clinical Epidemiology and Biostatistics, Children Health Advocacy Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

An early trend in the mean age of pubertal onset appears in adolescents, but the association between body fat percentage (BF%) of children and precocious puberty is unclear. The aim of the study was to analyze the association of sexual development with BF% in girls. A total of 407 females were included in this cross-sectional study. BF% was measured by Inbody S10, International Obesity Task Force was used to judge overweight or obesity, and early puberty was defined as a younger age than the median age in each of the pubertal Tanner stages. Logistic regression analysis was used to test relationships between pubertal states and independent variables, including age, weight, waist circumference (WC), type of school, and residency. Females with early puberty exhibited higher anthropometry data (such as weight, BMI, BF%) than females with normal maturation ( < 0.001). Weight, BMI, WC, BF% residency, and school type were related to pubertal state ( < 0.001). Females with higher BF% were more likely to exhibit early puberty (odds ratio = 1.138, 95% confidence interval = 1.046-1.237). The students who lived in urban areas and studied in public schools had a lower risk of early puberty. Moreover, BF% continuously increased with age in 6- to 9-year-old girls. Females with higher BF% may be more likely to exhibit early puberty. In future studies, more research is needed to analyze this mechanism of how BF% influences puberty development.
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http://dx.doi.org/10.1089/chi.2020.0247DOI Listing
June 2021

Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells.

Stem Cell Res Ther 2021 06 19;12(1):353. Epub 2021 Jun 19.

Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou, Zhejiang, PR China.

Background: Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system.

Methods: In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. The in vivo hematopoietic reconstitution potential of c-kit cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system.

Results: The c-kit cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201c-kitcells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201c-kitcells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. Moreover, CD201 hematopoietic cells remained in a relatively slow cycling state, consistent with high expression levels of P57 and Ccng2. Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 hematopoietic cell induction from mouse PSCs.

Conclusions: Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application.
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http://dx.doi.org/10.1186/s13287-021-02434-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214308PMC
June 2021

Boosting Chemodynamic Therapy by the Synergistic Effect of Co-Catalyze and Photothermal Effect Triggered by the Second Near-Infrared Light.

Nanomicro Lett 2020 Sep 2;12(1):180. Epub 2020 Sep 2.

State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry (CIAC), Chinese Academy of Sciences (CAS), Changchun, 130022, People's Republic of China.

In spite of the tumor microenvironments responsive cancer therapy based on Fenton reaction (i.e., chemodynamic therapy, CDT) has been attracted more attentions in recent years, the limited Fenton reaction efficiency is the important obstacle to further application in clinic. Herein, we synthesized novel FeO/MoS nanocomposites modified by bovine serum albumin (FeO/MoS-BSA) with boosted Fenton reaction efficiency by the synergistic effect of co-catalyze and photothermal effect of MoS nanosheets triggered by the second near-infrared (NIR II) light. In the tumor microenvironments, the MoS nanosheets not only can accelerate the conversion of Fe ions to Fe ions by Mo ions on their surface to improve Fenton reaction efficiency, but also endow FeO/MoS-BSA with good photothermal performances for photothermal-enhanced CDT and photothermal therapy (PTT). Consequently, benefiting from the synergetic-enhanced CDT/PTT, the tumors are eradicated completely in vivo. This work provides innovative synergistic strategy for constructing nanocomposites for highly efficient CDT.
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http://dx.doi.org/10.1007/s40820-020-00516-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7770794PMC
September 2020

To the Editor: Cavoportal Collateral Circulation in Budd-Chiari Syndrome.

Cardiovasc Intervent Radiol 2021 Jun 15. Epub 2021 Jun 15.

Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University, Chengdu, Sichuan, China.

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http://dx.doi.org/10.1007/s00270-021-02886-2DOI Listing
June 2021

Second-generation antipsychotics induce cardiotoxicity by disrupting spliceosome signaling: Implications from proteomic and transcriptomic analyses.

Pharmacol Res 2021 Jun 5;170:105714. Epub 2021 Jun 5.

Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. Electronic address:

Second-generation antipsychotics (SGAs) are first-line drugs that are prescribed for mental disorders in clinic. Severe cardiotoxicity has been widely reported and thus limits their clinical application. This study aimed to identify the common mechanism underlying SGAs-induced cardiotoxicity using dual-omics analyses. Balb/C mice were intraperitoneally injected with two representative SGAs, olanzapine (2.5 mg/kg) and clozapine (25 mg/kg), at clinically comparable doses for 0, 7, 14 and 21 days. Our results showed that both SGAs induced cardiomyocyte degeneration, inflammation infiltration, and cardiac fibrosis, all of which worsened with time. Proteomic analysis revelaed that 22 differentially expressed (DE) proteins overlapped in olanzapine and clozapine-treated hearts. These proteins were significantly enriched in muscle contraction, amino acid metabolism and spliceosomal assembly by GO term analysis and spliceosome signaling was among the top enriched pathways by KEGG analysis. Among the 22 DE proteins, three spliceosome signal proteins were validated in a dynamic detection, and their expression significantly correlated with the extent of SGAs-induced cardiac fibrosis. Following the spliceosome signaling dysregulation, RNA sequencing revealed that alternative splicing events in the mouse hearts were markedly enhanced by SGAs treatments, and the production of vast transcript variants resulted in dysregulation of multiple pathways that are critical for cardiomyocytes adaptation and cardiac remodeling. Pladienolide B, a specific inhibitor of mRNA splicing, successfully corrected SGAs-induced alternative splicing and significantly attenuated the secretion of pro-inflammatory factors and cell deaths induced by SGAs exposure. Our study concluded that the spliceosome signaling was a common pathway driving SGAs cardiotoxicity. Pharmacological inhibition of the spliceosome signaling represents a novel therapeutic strategy against SGAs cardiotoxicity.
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http://dx.doi.org/10.1016/j.phrs.2021.105714DOI Listing
June 2021

CCL17-CCR4 axis contributes to the onset of vitiligo in mice.

Immun Inflamm Dis 2021 Jun 2. Epub 2021 Jun 2.

Department of Dermatology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai'an, China.

Background: Destruction of melanocytes mediated by autoimmunity is currently believed as the main cause of vitiligo. This article aims to identify the role of CC chemokine ligand 17 (CCL17)-CC chemokine receptor 4 (CCR4) axis in vitiligo and provide new possibilities for the clinical treatment of vitiligo.

Methods: A total of 30 patients with vitiligo from Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University were recruited based on the inclusion and exclusion criteria. Trephine was used to obtain skin samples from the lesion area and its surrounding normal areas, and the expression levels of CCL17, CCR4, Tbx21, Eomes, and Blimp1 were determined by quantitative reverse transcription polymerase chain reaction. Vitiligo mouse model was established by adoptively transferring CFP-PMEL CD8+ T cells into sublethally irradiated Krt14-Kitl* mice. Recipient mice received intraperitoneal injection of 1 × 10 plaque-forming units of rVV-hPMEL on the same day of transfer. The degree of depigmentation was scored blindly by one observer 5 weeks after vitiligo induction. CFP-PMEL CD8+ T cells migration to skin, draining lymph nodes, spleen, and blood were detected by flow cytometry. CCR4 blockade was performed by intraperitoneal injection of neutralizing antibody.

Results: The expression levels of CCL17, CCR4, Tbx21, Eomes, and Blimp1 in skin lesions were significantly increased compared with that in surrounding normal areas. CCL17 and CCR4 mice exhibited significantly lower disease scores than WT mice. The CFP-PMEL CD8+ T cells accumulation was significantly decreased in the skin of CCL17 and CCR4 mice, but was not changed in draining lymph nodes, spleen, and blood. Administration of CCR4 neutralizing antibody decreased the degree of depigmentation and the recruitment of CFP-PMEL CD8+ T cells to the skin, while keeping the number of T cells in draining lymph nodes unchanged.

Conclusion: Targeting CCL17-CCR4 axis might inhibit T cell migrating to skin and alleviate vitiligo progression.
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http://dx.doi.org/10.1002/iid3.423DOI Listing
June 2021

Metabolic reprogramming of myeloid-derived suppressor cells: An innovative approach confronting challenges.

J Leukoc Biol 2021 Jun 2. Epub 2021 Jun 2.

Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Immune cells such as T cells, macrophages, dendritic cells, and other immunoregulatory cells undergo metabolic reprogramming in cancer and inflammation-derived microenvironment to meet specific physiologic and functional demands. Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that are characterized by immunosuppressive activity, which plays a key role in host immune homeostasis. In this review, we have discussed the core metabolic pathways, including glycolysis, lipid and fatty acid biosynthesis, and amino acid metabolism in the MDSCs under various pathologic situations. Metabolic reprogramming is a determinant of the phenotype and functions of MDSCs, and is therefore a novel therapeutic possibility in various diseases.
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http://dx.doi.org/10.1002/JLB.1MR0421-597RRDOI Listing
June 2021

Fullerene C60 Protects Against Intestinal Injury from Deoxynivalenol Toxicity by Improving Antioxidant Capacity.

Life (Basel) 2021 May 27;11(6). Epub 2021 May 27.

Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

Oxidative stress is involved in a wide variety of pathologies, and fullerene has been shown to have an antioxidant ability. Mycotoxins exert toxic effects through induction of excessive reactive oxygen species (ROS). Here, we evaluated water-soluble fullerene C60 for its anti-mycotoxin and antioxidant effects in vitro and in vivo. Intestinal epithelial cells were cultured with fullerene during deoxynivalenol (DON) exposure. The results revealed that fullerene C60 significantly promoted cell viability, decreased apoptosis and necrotic cell number, and significantly reduced intracellular ROS levels during DON exposure ( < 0.05). To investigate the role of fullerene in antioxidant capacity in vivo further, 40 three-week-old male C57BL/6 mice were randomly divided into four groups. There were no significant differences between the control and fullerene groups ( > 0.05). In mice exposed to DON, supplementation with fullerene C60 significantly improved growth performance, and enhanced the total antioxidant status and the activities of SOD and GPX in the intestine and liver ( < 0.05). In addition, fullerene C60 supplementation improved intestinal morphology, as indicated by a higher villus height and tight junction protein expression ( < 0.05). Furthermore, fullerene supplementation decreased serum concentrations of inflammatory cytokine and lipopolysaccharide (LPS; a penetrability marker) compared to the DON-challenged group ( < 0.05). The current study suggests that fullerene C60 improves intestinal antioxidant status against DON-induced oxidative stress in vitro and in vivo.
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http://dx.doi.org/10.3390/life11060491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229202PMC
May 2021

Model Decoupled Synchronization Control Design with Fractional Order Filter for H-Type Air Floating Motion Platform.

Entropy (Basel) 2021 May 19;23(5). Epub 2021 May 19.

School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430070, China.

H-type motion platform with linear motors is widely used in two-degrees-of-freedom motion systems, and one-direction dual motors need to be precisely controlled with strict synchronization for high precision performance. In this paper, a synchronous control method based on model decoupling is proposed. The dynamic model of an H-type air floating motion platform is established and one direction control using two motors with position dependency coupling is decoupled and converted into independent position and rotation controls, separately. For the low damping second-order oscillation system of the rotation control loop, a new fractional order biquad filtering method is proposed to generate an antiresonance peak to improve the phase and control gain of the open loop system, which can ensure system stability and quick attenuation for external disturbances. In the multiple-degree-of-freedom decoupled control loops, a systematic feedback controller design methodology is proposed to satisfy the given frequency domain design specifications; a feed-forward control strategy is also applied to compensate the disturbance torque caused by the platform motion. The simulation and experimental results demonstrate that the proposed synchronization control method is effective, and achieves better disturbance rejection performance than the existing optimal cancellation filtering method and biquad filtering method.
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http://dx.doi.org/10.3390/e23050633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159128PMC
May 2021

Big Five Personality Model-based study of death coping self-efficacy in clinical nurses: A cross-sectional survey.

PLoS One 2021 27;16(5):e0252430. Epub 2021 May 27.

Department of Nursing, Zhejiang University School of Medicine Sir Run Run Shaw Hospital, Hangzhou, Zhejiang, China.

Background: Specific personality traits may affect the ability of nurses to deal with patient death. The relationship between personality and death coping self-efficacy (DCS) has rarely been investigated in the palliative care setting. In this study, we explored the associations between different personality profiles and DCS in clinical nurses from general wards and ICU.

Methods: A cross-sectional survey of 572 Chinese nurses was conducted between August and September 2020, by way of a self-administered questionnaire.

Results: Among the Big Five Personality Traits, in nurses the score was highest for conscientiousness and lowest for neuroticism. With regard to DCS, nurses scored highly on the intention of hospice care. The Big Five Personality Traits were found to explain 20.2% of the overall variation in DCS. Openness, agreeableness and conscientiousness were significantly associated with DCS in nurses.

Conclusions: Nursing managers should pay attention to differences in personality characteristics and provide personalized and targeted nursing education. This should improve nurses' DCS, enrich their professional development and promote high quality palliative care for patients and their families.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252430PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158956PMC
May 2021

Artificial Intelligence for Screening Chinese Electronic Medical Record and Biobank Information.

Biopreserv Biobank 2021 May 26. Epub 2021 May 26.

Department of Biobank, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

To establish a structured and integrated platform of clinical data and biobank data, and a client to retrieve these data. Initially, the hospital information system (HIS) and biobank information system (BIS) were integrated through the patients' ID numbers. Then, natural language processing (NLP) was used to process the integrated unstructured clinical information. A query interface was designed for this system, which enabled researchers to retrieve clinical or biobank data. Finally, several queries were listed and manually checked to test the retrieval performance of the system. The construction of the biobank screening system (BSS) was completed, and the data were structured. The BSS took an average of 2 seconds to perform a search for target patients/samples. The retrieval results were consistent with the HIS and BIS. For complex queries, we manually checked the retrieved patients/samples, and the system's accuracy was 100%. This NLP-based system improved biological sample screening and using of clinical data. We will continue to improve this system, enhance resource sharing, and promote the development of translational medicine.
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http://dx.doi.org/10.1089/bio.2020.0151DOI Listing
May 2021

Novel Algorithms for Comprehensive Untargeted Detection of Doping Agents in Biological Samples.

Anal Chem 2021 06 21;93(21):7746-7753. Epub 2021 May 21.

Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center Campus, 382 West Street Road, Kennett Square, Pennsylvania 19348, United States.

To address the limitations of current targeted analytical methods that can only detect known doping agents, a novel methodology that permits untargeted drug detection (UDD) has been developed to help in the fight against doping in sports. Fifty-seven drugs were spiked into blank equine plasma and were treated as unknowns since their exact masses and chromatographic retention times were not utilized for detection. The spiked drugs were extracted from the plasma samples and were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). The acquired LC-HRMS raw data files were processed using metabolomic software for compound detection and identification. For UDD with the resultant data, a mathematical model was created, and two algorithms were generated to calculate the ratio of the mean (ROM) and outlier index (OLI). Using ROM and OLI, the majority of the 57 drugs were accurately detected by name (52 of 57) or chemical formula (1 of 57). The limit of detection for the drugs was from tens of picograms to nanograms per milliliter. Xenobiotics and endogenous substances relevant to doping control were also identified using this untargeted approach following their extraction from real-world race samples, thus validating the UDD methodology. To the authors' knowledge, this is the first completely UDD methodological approach and represents significant advance toward using artificial intelligence for the detection of both known and emerging doping agents in sports.
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http://dx.doi.org/10.1021/acs.analchem.1c01273DOI Listing
June 2021

Degradation of blue honeysuckle polysaccharides, structural characteristics and antiglycation and hypoglycemic activities of degraded products.

Food Res Int 2021 05 9;143:110281. Epub 2021 Mar 9.

College of Art and Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

In this study, HO-Vc (hydrogen peroxide-ascorbic acid) oxidation system under 50 and 70 °C was developed to degrade blue honeysuckle polysaccharides. The results suggested that viscosity-average molecular weight was decreased and reducing sugar content was raised with the rise of time or temperature. The degradation was fitted to the second-order reaction kinetics. The gas chromatography revealed two degraded polysaccharides were consisted of six monosaccharides (Gal A, Rha, Ala, Gal, Glu, Man) with different ratios, and eight types of sugar residues were confirmed using nuclear magnetic resonance. Based on the linearity (R), branching (R), branch size (R) calculation, PD70 (purified degraded polysaccharide at 70 °C) had a more linear structure and longer side chains comparing to PD50 (purified degraded polysaccharide at 50 °C). The absence of triple helical structure and sheet-like aggregation with rough surface were confirmed by the Congo red test and scanning electron. Rheological characterization proved the two degraded polysaccharides exhibited shear-thinning behavior and viscoelastic property. Besides, the two degraded polysaccharides displayed strong antiglycation activities, inhibitory effects against α-amylase and α-glucosidase, and exhibited competitive inhibitory kinetics. These findings support the potential application of blue honeysuckle polysaccharides as the treatment of diabetes.
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http://dx.doi.org/10.1016/j.foodres.2021.110281DOI Listing
May 2021

IL-17-producing γδT cells ameliorate intestinal acute graft-versus-host disease by recruitment of Gr-1CD11b myeloid-derived suppressor cells.

Bone Marrow Transplant 2021 May 13. Epub 2021 May 13.

Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Acute graft-versus-host disease (aGVHD) is the most severe complication and the major cause of morbidity and mortality in patients who have received hematopoietic stem cell transplantation. Inflammation mediated by donor T cells and neighboring recipient cells is considered to be responsible for intestinal aGVHD. Interleukin (IL)-17A-producing γδT (γδT17) cells have been investigated as key players in cancer, immunity, and inflammatory responses because of their phenotypic plasticity, memory-like activity, and unique migratory features. However, the precise roles and underlying mechanisms of γδT17 cells in aGVHD immunopathogenesis remain elusive. Here, we found that γδT17 cells constituted the major resident γδT population in the intestinal lamina propria of aGVHD mice. Adoptive infusion of induced γδT17 cells markedly attenuated murine aGVHD and increased infiltration of Gr-1CD11b myeloid-derived suppressor cells (MDSCs) into the inflammatory intestine, and did not affect graft-versus-leukemia(GVL) effect. Further experiments indicated that γδT17 cells enhanced both migration ability and immunosuppressive activity of MDSCs. γδT17 cell-mediated protection in aGVHD was blunted by depletion of IL-17A or MDSCs. Our study clarifies an immune pathway where γδT17 cells play a protective role in murine aGVHD by recruiting MDSCs to the inflammatory intestine.
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http://dx.doi.org/10.1038/s41409-021-01299-6DOI Listing
May 2021

Chemical Identification of Catalytically Active Sites on Oxygen-doped Carbon Nanosheet to Decipher the High Activity for Electro-synthesis Hydrogen Peroxide.

Angew Chem Int Ed Engl 2021 Jul 17;60(30):16607-16614. Epub 2021 Jun 17.

State Key Laboratory of Powder Metallurgy, School of Physical and Electronics, Central South University, 410083, Changsha, China.

Electrochemical production of hydrogen peroxide (H O ) through two-electron (2 e ) oxygen reduction reaction (ORR) is an on-site and clean route. Oxygen-doped carbon materials with high ORR activity and H O selectivity have been considered as the promising catalysts, however, there is still a lack of direct experimental evidence to identify true active sites at the complex carbon surface. Herein, we propose a chemical titration strategy to decipher the oxygen-doped carbon nanosheet (OCNS ) catalyst for 2 e ORR. The OCNS exhibits outstanding 2 e ORR performances with onset potential of 0.825 V (vs. RHE), mass activity of 14.5 A g at 0.75 V (vs. RHE) and H O production rate of 770 mmol g  h in flow cell, surpassing most reported carbon catalysts. Through selective chemical titration of C=O, C-OH, and COOH groups, we found that C=O species contributed to the most electrocatalytic activity and were the most active sites for 2 e ORR, which were corroborated by theoretical calculations.
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http://dx.doi.org/10.1002/anie.202104480DOI Listing
July 2021

Experimental evolution in morbidostat reveals converging genomic trajectories on the path to triclosan resistance.

Microb Genom 2021 May;7(5)

Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.

Understanding the dynamics and mechanisms of acquired drug resistance across major classes of antibiotics and bacterial pathogens is of critical importance for the optimization of current anti-infective therapies and the development of novel ones. To systematically address this challenge, we developed a workflow combining experimental evolution in a morbidostat continuous culturing device with deep genomic sequencing of population samples collected in time series. This approach was applied to the experimental evolution of six populations of BW25113 towards acquiring resistance to triclosan (TCS), an antibacterial agent in various consumer products. This study revealed the rapid emergence and expansion (up to 100% in each culture within 4 days) of missense mutations in the gene, encoding enoyl-acyl carrier protein reductase, the known TCS molecular target. A follow-up analysis of isolated clones showed that distinct amino acid substitutions increased the drug IC in a 3-16-fold range, reflecting their proximity to the TCS-binding site. In contrast to other antibiotics, efflux-upregulating mutations occurred only rarely and with low abundance. Mutations in several other genes were detected at an earlier stage of evolution. Most notably, three distinct amino acid substitutions were mapped in the C-terminal periplasmic domain of CadC protein, an acid stress-responsive transcriptional regulator. While these mutations do not confer robust TCS resistance, they appear to play a certain, yet unknown, role in adaptation to relatively low drug pressure. Overall, the observed evolutionary trajectories suggest that the FabI enzyme is the sole target of TCS (at least up to the ~50 µm level), and amino acid substitutions in the TCS-binding site represent the main mechanism of robust TCS resistance in . This model study illustrates the potential utility of the established morbidostat-based approach for uncovering resistance mechanisms and target identification for novel drug candidates with yet unknown mechanisms of action.
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http://dx.doi.org/10.1099/mgen.0.000553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209735PMC
May 2021

Targeting the EMT transcription factor Snail overcomes resistance to osimertinib in EGFR-mutant non-small cell lung cancer.

Thorac Cancer 2021 06 4;12(11):1708-1715. Epub 2021 May 4.

Department of Oncology, Tianjin Medical University General Hospital, Tianjin, China.

Background: The resistance mechanism of the third generation of epidermal growth factor (EGFR) tyrosine kinase inhibitor (TKI) osimertinib is complex. Epithelial mesenchymal transition (EMT) is a common mechanism of EGFR-TKI acquired resistance. Snail is an important transcription factor related to EMT. Whether targeting Snail can reverse the resistance of osimertinib by downregulating Snail is unknown.

Methods: The presence of EMT in H1975/OR (osimertinib resistance) cells was confirmed by transwell assay. To explore the EMT role in resistance, the expression levels of EMT markers were detected in both parental cells H1975 and resistant cells H1975OR. We used RNA interference technology to knockdown the key regulator Snail in resistant cells. After the interference efficiency was confirmed, changes in EMT-related molecules of Snail were explicitly downregulated, and changes in sensitivity and migration and invasion ability were also examined. We used CDK4/6 inhibitor to test the ability of reversing drug resistance by downregulating Snail.

Results: Compared with the H1975 cell line, the H1975/OR resistant cell line showed increased invasiveness, upregulated expression of vimentin and downregulation of E-cadherin. EMT occurred in the H1975/OR resistant cell line. The expression of Snail was upregulated in the osimertinib-resistant cell line H1975/OR. Knockdown of Snail increased the sensitivity of H1975/OR cells to osimertinib. CDK4/6 inhibitor palbociclib could downregulate the expression of Snail. CDK 4/6 inhibitor palbociclib combined with osimertinib could reverse the resistance of osimertinib in H1975/OR.

Conclusions: Snail plays an important role in the third generation of EGFR-TKI osimertinib resistance, which may be reversed by downregulating Snail.
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http://dx.doi.org/10.1111/1759-7714.13906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169301PMC
June 2021

Current status of aspiration thrombectomy for acute stroke patients in China: data from ANGEL-ACT Registry.

Ther Adv Neurol Disord 2021 12;14:17562864211007715. Epub 2021 Apr 12.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, 100070, China.

Background And Aims: Although noninferior to stent retriever (SR) as first-line approach for endovascular treatment (EVT) of acute large vessel occlusion (LVO) stroke, little is known about the current status of direct aspiration (DA) as first-line thrombectomy in China. This analysis of a prospective, nationwide registry (ANGEL-ACT) aimed to investigate the prevalence and comparative effectiveness of DA-first thrombectomy in a real-world practice in China.

Methods: All patients receiving thrombectomy were screened from a prospective cohort of LVO patients undergoing EVT at 111 hospitals in China between November 2017 and March 2019, and divided into two groups based upon which type of thrombectomy was attempted first ("DA-first" and "SR-first"). The following outcome measures were compared using logistic regression models with adjustment: successful recanalization after first-device alone and all procedures, use of rescue treatment, intracranial hemorrhage (ICH) within 24 h, and modified Rankin Scale (mRS) score at 90 days.

Results: A total of 1225 patients, 102 (8.3%) in DA-first group and 1123 (91.7%) in SR-first group, were included. Patients receiving DA-first had less often successful recanalization after first-device alone [30.4 66.4%; odds ratio (OR) = 0.23, 95% confidence interval (CI) = 0.15-0.37], more frequent rescue treatment (62.8 27.0%; OR = 4.55, 95% CI = 2.92-7.08) and ICH (35.4 22.1%; OR = 1.78, 95% CI = 1.12-2.83) than those receiving SR-first; however, no significant difference was found in successful recanalization after all procedures (84.3 90.3%;  = 0.18) and 90-day mRS (median: 3 3 points;  = 0.90) between both groups.

Conclusion: This real-world registry suggested that DA-first thrombectomy for acute stroke patients lagged behind in China during the study period. Far fewer DA-first than SR-first thrombectomies were performed, and DA-first was associated with lower first-device recanalization, more frequently requiring rescue treatment, and increased ICH risk.

Clinical Trial Registration: ClinicalTrials.gov, NCT03370939.
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http://dx.doi.org/10.1177/17562864211007715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047968PMC
April 2021

Photoelectrochemical detection of human epidermal growth factor receptor 2 (HER2) based on CoO-ascorbic acid oxidase as multiple signal amplifier.

Mikrochim Acta 2021 Apr 20;188(5):166. Epub 2021 Apr 20.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China.

A sensitive photoelectrochemical (PEC) sensor based on hexagonal carbon nitride tubes (HCNT) as photoactive material was prepared for the detection of human epidermal growth factor receptor 2 (HER2). Magnetic FeO nanospheres (MNs) modified with anti-HER2 antibodies were employed for highly efficient capture of HER2 from serum sample, and CoO nanoparticles (CoO NPs) modified with ascorbic acid oxidase (AAO) as well as HER2 aptamer were used for signal amplification. When the aptamer-CoO-AAO probe was captured onto the electrode surface through the specific binding of the aptamer with HER2, the photocurrent intensity decreased. This was because CoO NPs competed with HCNT for consumption of the excitation energy. As a consequence AAO catalyzed the oxidation of the electron donor (AA), and the aptamer-CoO-AAO probe increased the steric hindrance at the electrode surface, leading to significant photocurrent intensity decrease, thus realizing multiple signal amplification. Based on this signal amplification strategy, at 0 V (vs Ag/AgCl), the PEC sensor shows a wide linear response ranging from 1 pg mL to 1 ng mL with a low detection limit of 0.026 pg mL for HER2. Importantly, the prepared PEC sensor was applied for detection of HER2 in human serum samples with recoveries between 98.8 and 101%. Sensitive photoelectrochemical sensor based on CoO nanoparticles modified with ascorbic acid oxidase for signal amplification is reported.
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http://dx.doi.org/10.1007/s00604-021-04829-7DOI Listing
April 2021

Association of small intestinal bacterial overgrowth with Parkinson's disease: a systematic review and meta-analysis.

Gut Pathog 2021 Apr 16;13(1):25. Epub 2021 Apr 16.

Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Objective: Parkinson's disease (PD) is the second most prevalent neurodegenerative disease after Alzheimer's disease (AD) worldwide. The prevalence of small intestinal bacterial overgrowth (SIBO) in PD patients is high. We conducted this comprehensive systematic review and meta-analysis to determine the association between SIBO and PD.

Methods: A comprehensive literature search of the PubMed, Cochrane Library and EMBASE databases was performed to identify studies correlating SIBO with PD. Studies were screened, and relevant data were extracted and analysed. We calculated the pooled prevalence of SIBO in all individuals with PD and compared the prevalence of SIBO between the two groups to calculate an odds ratio (OR) and 95% confidence interval (CI). Egger's test was performed to assess publication bias.

Results: Eleven studies with 973 participants met the inclusion criteria. The pooled prevalence of SIBO in patients with PD was 46% (95% CI 36-56). A random-effects model was applied given the heterogeneity (I = 83%) detected among the studies. Egger's test indicated no publication bias (p = 0.0657). Subgroup analyses showed that the prevalence of SIBO was greater in studies including patients diagnosed using the lactulose hydrogen breath test (LBT) (51%, 95% CI 37-65) than in those including patients diagnosed using the glucose hydrogen breath test (GBT) (35%, 95% CI 20-50), and the prevalence of SIBO in PD was highest (55%, 95% CI 38-72) in patients diagnosed by the LBT and GBT. The prevalence of SIBO was 52% (95% CI 40-64) among patients from Western countries and 33% (95% CI 22-43) among patients from Eastern countries. The pooled OR of SIBO in PD patients compared with healthy controls was 5.22 (95% CI 3.33-8.19, p < 0.00001). We did not identify an obvious predictor of SIBO in PD patients.

Conclusion: In conclusion, our meta-analysis found a strong association between SIBO and PD with approximately half of PD patients testing positive for SIBO. These relationships significantly differed based on diagnostic test and geographic area.
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http://dx.doi.org/10.1186/s13099-021-00420-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8051095PMC
April 2021

Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2-M checkpoint modulation.

Cancer Med 2021 05 30;10(9):3101-3112. Epub 2021 Mar 30.

Suzhou Cancer Center Core Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, Jiangsu, P.R. China.

Background: Radiotherapy is one of the main strategies for the treatment of esophageal squamous cell carcinoma (ESCC). However, treatment failure often occurs due to the emergence of radioresistance. In this study, we report a key regulator of radiation sensitivity, termed TAB182 that may become an ideal biomarker and therapeutic target to overcome radioresistance.

Materials And Methods: By applying qRT-PCR and immunohistochemical staining, the expression of TAB182 was detected in patient tissues. We next assessed the influence of TAB182 downregulation to radiosensitivity using clonogenic survival assay and γ-H2A.X foci analysis in TE-1, TE-10, and radioresistant TE-1R cell lines after ionizing radiation. To unveil the mechanism underlying, TAB182 interacting proteins were identified by mass spectrometry following co-immunoprecipitation. Furthermore, flow cytometry and western blot assay were applied to validate the identified proteins.

Results: Our results demonstrated that the expression of TAB182 is higher in cancer tissues than normal tissues and elevated expression of TAB182 correlates with poor outcomes of postoperative radiotherapy. Downregulation of TAB182 sensitized cancer cells to ionizing radiation, particularly in radioresistant TE-1R cells that spontaneously overexpress TAB182. Mechanically, TAB182 interacts with FHL2 to induce G2-M arrest through wiring the CHK2/CDC25C/CDC2 signaling pathway. Finally, overexpression of shRNA-resistant TAB182 restored the checkpoint and radioresistance.

Conclusion: TAB182 potentiates the radioresistance of ESCC cells by modulating the G2-M checkpoint through its interaction with FHL2. Thus, TAB182 may become an ideal biomarker and therapeutic target of ESCC radiotherapy.
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http://dx.doi.org/10.1002/cam4.3879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085956PMC
May 2021

Insoluble matrix proteins from shell waste for synthesis of visible-light response photocatalyst to mineralize indoor gaseous formaldehyde.

J Hazard Mater 2021 08 17;415:125649. Epub 2021 Mar 17.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR China; Xi'an High-Tech Institute, Xi'an 710025, PR China. Electronic address:

HCHO is the most concerned indoor air pollutant that photocatalytic degradation is a feasible approach. To achieve efficient and complete degradation of HCHO under visible light irradiation, heteroatoms are usually doped in TiO. But using natural materials as a dopant instead of expensive and toxic chemicals to fertilize TiO remains challenging. This paper proposes a sustainable and green approach to synthesize an efficient N, Ca co-doped TiO photocatalyst (TIMP) by using the insoluble matrix proteins (IMPs) extracted from abalone shell. TIMP-0.8 achieves near completely degradation HCHO within 45 min under visible light at ambient temperature and exhibits superior stability after 7 cycles. TIMP-0.8 has monodispersity with smaller diameter, high porosity, abundant defects and high adsorption affinity for surface hydroxyls compared with pure TiO. With the assistance of IMPs, the rate-determining step of HCHO degradation changes from -COOH oxidation to spontaneous decomposition of HCO, significantly facilitating the elimination and mineralization of HCHO. Overall, IMPs from abalone shell are natural surfactant, bio-templet, and dopant for TiO modification, contributing to desirable visible-light photocatalytic performance for HCHO degradation. This paper provides new insight for high-value utilization of waste shell and photocatalytic indoor purification.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125649DOI Listing
August 2021

Artificial intelligence-assisted reduction in patients' waiting time for outpatient process: a retrospective cohort study.

BMC Health Serv Res 2021 Mar 17;21(1):237. Epub 2021 Mar 17.

School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Many studies suggest that patient satisfaction is significantly negatively correlated with the waiting time. A well-designed healthcare system should not keep patients waiting too long for an appointment and consultation. However, in China, patients spend notable time waiting, and the actual time spent on diagnosis and treatment in the consulting room is comparatively less.

Methods: We developed an artificial intelligence (AI)-assisted module and name it XIAO YI. It could help outpatients automatically order imaging examinations or laboratory tests based on their chief complaints. Thus, outpatients could get examined or tested before they went to see the doctor. People who saw the doctor in the traditional way were allocated to the conventional group, and those who used XIAO YI were assigned to the AI-assisted group. We conducted a retrospective cohort study from August 1, 2019 to January 31, 2020. Propensity score matching was used to balance the confounding factor between the two groups. And waiting time was defined as the time from registration to preparation for laboratory tests or imaging examinations. The total cost included the registration fee, test fee, examination fee, and drug fee. We used Wilcoxon rank-sum test to compare the differences in time and cost. The statistical significance level was set at 0.05 for two sides.

Results: Twelve thousand and three hundred forty-two visits were recruited, consisting of 6171 visits in the conventional group and 6171 visits in the AI-assisted group. The median waiting time was 0.38 (interquartile range: 0.20, 1.33) hours for the AI-assisted group compared with 1.97 (0.76, 3.48) hours for the conventional group (p < 0.05). The total cost was 335.97 (interquartile range: 244.80, 437.60) CNY (Chinese Yuan) for the AI-assisted group and 364.58 (249.70, 497.76) CNY for the conventional group (p < 0.05).

Conclusions: Using XIAO YI can significantly reduce the waiting time of patients, and thus, improve the outpatient service process of hospitals.
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http://dx.doi.org/10.1186/s12913-021-06248-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966905PMC
March 2021

Use of high resolution/accurate mass full scan/data-dependent acquisition for targeted/non-targeted screening in equine doping control.

Anal Methods 2021 04 12;13(13):1565-1575. Epub 2021 Mar 12.

Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, New Bolton Center Campus, Kennett Square, PA 19348, USA.

High-resolution mass spectrometry (HRMS) is a very powerful technology for equine doping control analysis. The more recently developed hybrid type of Orbitrap-based HRMS instrument allows for both targeted and non-targeted screening analyses in a single liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS/MS) run. In the present study, an LC-HRMS/MS method was developed and validated to detect prohibited substances in equine sports. The substances were recovered from equine plasma by liquid-liquid extraction (LLE) using methyl tert-butyl ether and were separated on a C18 reversed-phase column using mobile phases of 5 mM ammonium formate and acetonitrile. A 7.5 min LC gradient was employed to elute substances and results indicated that the LC method generated sharp and symmetric chromatographic peaks. An in-house equine doping compound database and a spectral library were built to increase method specificity for substances of interest. Five criteria, i.e. accurate mass, retention time, isotope pattern, selected HRMS/MS fragment ions (compound database) and HRMS/MS spectra (spectral library), were employed for targeted screening. We utilized these criteria to validate targeted detection of 451 substances within our in-house equine doping compound database. By using all five criteria in screening, the false screening positive rate is significantly reduced. A screening strategy and a Microsoft Excel macro were developed to facilitate interpretation and reporting of results. As the simultaneous acquisition of the full scan HRMS data provides the opportunity for retrospective non-targeted analysis, our findings highlight the use of this novel methodology as a simple, rapid, and reliably reproducible strategy to meet the challenge of identifying an increasing number of doping substances that could potentially impact the integrity of the horse racing community.
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http://dx.doi.org/10.1039/d0ay02297gDOI Listing
April 2021

CircRNA circ_0067772 aggravates the malignant progression of cutaneous squamous cell carcinoma by regulating miR-1238-3p/FOXG1 axis.

Genes Genomics 2021 May 11;43(5):491-501. Epub 2021 Mar 11.

Department of Dermatology, The Affiliated Huaian NO.1 People's Hospital of Nanjing Medical University, No. 6, West Beijing Road, Huaiyin District, Huaian, 223300, Jiangsu, China.

Background: Cutaneous squamous cell carcinoma (CSCC) is a severe malignancy derived from skin. Dysregulated circular RNAs (circRNAs) might play vital roles in tumor development.

Objective: Here, we aimed to explore the function of a novel circRNA circ_0067772 in CSCC.

Methods: Quantitative real-time PCR (qRT-PCR) or Western blot assay was performed to determine the expression of circ_0067772, microRNA (miR)-1238-3p and forkhead box protein G1 (FOXG1). Cell proliferation was assessed by Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Transwell assay and wound healing assay were employed to examine cell metastasis. Flow cytometry was employed to monitor cell cycle and apoptosis. The target association between miR-1238-3p and circ_0067772 or FOXG1 was validated by dual-luciferase reporter assay. Moreover, role of circ_0067772 in vivo was investigated via xenograft model in nude mice.

Results: Circ_0067772 and FOXG1 were upregulated, while miR-1238-3p was downregulated in CSCC tissues and cells. Circ_0067772 knockdown conferred inhibitory effects on cell proliferation, migration and invasion of CSCC cells. MiR-1238-3p served as a target of circ_0067772, whose silencing could reverse circ_0067772 knockdown-induced inhibitory impact on the malignant cellular behaviors. Circ_0067772 positively regulated FOXG1 expression by antagonizing miR-1238-3p. Additionally, miR-1238-3p could repress CSCC cell proliferation, migration and invasion by targeting FOXG1. Also, circ_0067772 knockdown hindered CSCC tumor growth in vivo.

Conclusion: Our study identified a novel oncogenic circRNA and the involvement of circ_0067772/miR-1238-3p/FOXG1 axis in CSCC development, providing a target for CSCC therapy.
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http://dx.doi.org/10.1007/s13258-021-01074-3DOI Listing
May 2021