Publications by authors named "Xiaoqin Zhang"

194 Publications

Changes in blood pressure and related risk factors among nurses working in a negative pressure isolation ward.

Front Public Health 2022 22;10:942904. Epub 2022 Jul 22.

Nursing Department, Guangdong Second Provincial General Hospital, Guangzhou, China.

Objective: To observe changes in blood pressure (ΔBP) and explore potential risk factors for high ΔBP among nurses working in a negative pressure isolation ward (NPIW).

Methods: Data from the single-center prospective observational study were used. Based on a routine practice plan, female nurses working in NPIW were scheduled to work for 4 days/week in different shifts, with each day working continuously for either 5 or 6 h. BP was measured when they entered and left NPIW. Multivariable logistic regression was used to assess potential risk factors in relation to ΔBP ≥ 5 mm Hg.

Results: A total of 84 nurses were included in the analysis. The ΔBP was found to fluctuate on different working days; no significant difference in ΔBP was observed between the schedules of 5 and 6 h/day. The standardized score from the self-rating anxiety scale (SAS) was significantly associated with an increased risk of ΔBP ≥ 5 mm Hg (odds ratio [OR] = 1.12, 95% CI: 1.00-1.24). Working 6 h/day (vs. 5 h/day) in NPIW was non-significantly related to decreased risk of ΔBP (OR = 0.70), while ≥ 2 consecutive working days (vs. 1 working day) was non-significantly associated with increased risk of ΔBP (OR = 1.50).

Conclusion: This study revealed no significant trend for ΔBP by working days or working time. Anxiety was found to be significantly associated with increased ΔBP, while no <2 consecutive working days were non-significantly related to ΔBP. These findings may provide some preliminary evidence for BP control in nurses who are working in NPIW for Coronavirus Disease 2019 (COVID-19).
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http://dx.doi.org/10.3389/fpubh.2022.942904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353044PMC
August 2022

Genome-Wide Identification of R2R3-MYB Transcription Factor and Expression Analysis under Abiotic Stress in Rice.

Plants (Basel) 2022 Jul 25;11(15). Epub 2022 Jul 25.

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

The myeloblastosis (MYB) family comprises a large group of transcription factors (TFs) that has a variety of functions. Among them, the R2R3-MYB type of proteins are the largest group in plants, which are involved in controlling various biological processes such as plant growth and development, physiological metabolism, defense, and responses to abiotic and biotic stresses. In this study, bioinformatics was adopted to conduct genome-wide identification of the R2R3-MYB TFs in rice. We identified 190 MYB TFs (99 R2R3-MYBs), which are unevenly distributed on the 12 chromosomes of rice. Based on the phylogenetic clustering and protein sequence characteristics, OsMYBs were classified into five subgroups, and 59.6% of the genes contained two introns. Analysis of cis-acting elements in the 2000 bp upstream region of genes showed that all genes contained plant hormones-related or stress-responsive elements since 91.9%, 79.8%, 79.8%, and 58.6% of genes contain ABRE, TGACG, CGTCA, and MBS motifs, respectively. Protein-protein network analysis showed that the were involved in metabolic process, biosynthetic process, and tissue development. In addition, some genes showed a tissue-specific or developmental-stage-specific expression pattern. Moreover, the transcription levels of 20 genes under polyethylene glycol (PEG) and cadmium chloride (CdCl) stress inducers were dissected by qRT-PCR. The results indicated genes with an altered expression upon PEG or CdCl stress induction. These results potentially supply a basis for further research on the role that genes play in plant development and stress responses.
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http://dx.doi.org/10.3390/plants11151928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330779PMC
July 2022

Lysyl oxidase promotes renal fibrosis via accelerating collagen cross-link driving by β-arrestin/ERK/STAT3 pathway.

FASEB J 2022 Aug;36(8):e22427

Department of Nephrology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Lysyl oxidase (LOX) is a copper-dependent monoamine oxidase whose primary function is the covalent cross-linking of collagen in the extracellular matrix (ECM). Evidence has shown that LOX is associated with cancer and some fibrotic conditions. We recently found that serum LOX is a potential diagnostic biomarker for renal fibrosis, but the mechanism by which LOX is regulated and contributes to renal fibrosis remains unknown. The current study demonstrates the following: (1) LOX expression was increased in fibrotic kidneys including ischemia-reperfusion injury-(IRI-), unilateral ureteral obstruction-(UUO-), and folic acid- (FA-) induced fibrotic kidneys as well as in the paraffin-embedded sections of human kidneys from the patients with renal fibrosis. (2) The increasing deposition and cross-linking of collagen induced by LOX was observed in IRI-, UUO- and FA-kidneys. (3) LOX was regulated by the β-arrestin-ERK-STAT3 pathway in renal fibrosis. STAT3 was the downstream of AT1R-β-arrestin-ERK, ERK entered the nucleus and activated STAT3-pY705 but not STAT3-pS727. (4) STAT3 nuclear subtranslocation and binding to the LOX promoter may be responsible for the upregulation of LOX expression. (5) Pharmacologic inhibition of LOX with BAPN in vivo inhibited the upregulation of LOX, decreased collagen over cross-linking and ameliorated renal fibrosis after ischemic injury. Collectively, these observations suggest that LOX plays an essential role in the development of renal fibrosis by catalyzing collagen over cross-linking. Thus, strategies targeting LOX could be a new avenue in developing therapeutics against renal fibrosis.
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http://dx.doi.org/10.1096/fj.202200573RDOI Listing
August 2022

Cross talk between lysine methyltransferase Smyd2 and TGF-β-Smad3 signaling promotes renal fibrosis in autosomal dominant polycystic kidney disease.

Am J Physiol Renal Physiol 2022 08 27;323(2):F227-F242. Epub 2022 Jun 27.

Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota.

Autosomal dominant polycystic kidney disease (ADPKD) is an inherited genetic disorder that is caused by mutations in or genes and is characterized by renal fluid-filled cyst formation and interstitial fibrosis. gene mutation results in the upregulation of SET (suppressor of variegation, enhancer of zeste, trithorax) and MYND (myeloid-nervy-DEAF1) domain-containing lysine methyltransferase 2 (SMYD2) in kidneys from mutant mice and patients with ADPKD. However, the role and mechanism of Smyd2 in the regulation of renal fibrosis in ADPKD remains elusive. In the present study, we showed that ) expression of Smyd2 can be regulated by transforming growth factor (TGF)-β-Smad3 in normal rat kidney 49F (NRK-49F) cells and mouse fibroblast NIH3T3 cells; ) knockdown of Smyd2 and inhibition of Smyd2 with its specific inhibitor, AZ505, decreases TGF-β-induced expression of α-smooth muscle actin, fibronectin, collagen type 1 and 3, and plasminogen activator inhibitor-1 in NRK-49F cells; ) Smyd2 regulates the transcription of fibrotic marker genes through binding on the promoters of those genes or through methylating histone H3 to indirectly regulate the expression of those genes; and ) knockout and inhibition of Smyd2 significantly decreases renal fibrosis in knockout mice, supporting that targeting Smyd2 can not only delay cyst growth but also attenuate renal fibrosis in ADPKD. This study identified a cross talk between TGF-β signaling and Smyd2 in the regulation of fibrotic gene transcription and activation of fibroblasts in cystic kidneys, suggesting that targeting Smyd2 with AZ505 is a potential therapeutic strategy for ADPKD treatment. Here, we identified a cross talk between SET and MYND domain-containing lysine methyltransferase 2 (Smyd2) and transforming growth factor (TGF)-β-Smad3 signaling and a synergistic feedback loop between them, in which TGF-β stimulates expression of Smyd2 in a Smad3-dependent manner, and upregulation of Smyd2 regulates the transcription of TGF-β and other fibrotic marker genes through direct binding on their promoters or methylating histone H3 indirectly to regulate the transcription of those genes in fibroblasts. Thus, the Smyd2-TGF-β-Smad3-Smyd2 signaling axis plays an important role in promoting renal fibrosis, and targeting Smyd2 with its specific inhibitor should not only delay cyst growth but also ameliorate renal fibrosis in ADPKD.
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http://dx.doi.org/10.1152/ajprenal.00452.2021DOI Listing
August 2022

Acupoint Massage plus Recombinant Bovine Basic Fibroblast Growth Factor Ophthalmic Gel and Limbal Stem Cell Transplantation on Visual Quality, Corneal Refraction, and Aesthetic Outcome in Patients with Pterygium.

Authors:
Xiaoqin Zhang Yu Wu

Evid Based Complement Alternat Med 2022 14;2022:7103496. Epub 2022 Jun 14.

Department of Ophthalmology, People's Hospital of Lujiang County, Hefei, Anhui, China.

Objective: To investigate the clinical study of acupoint massage combined with recombinant bovine basic fibroblast growth factor (rbFGF) ophthalmic gel combined with limbal stem cell transplantation on visual quality, corneal refraction, and aesthetic outcome in patients with pterygium.

Methods: Sixty patients with pterygium treated in People's Hospital of Lujiang County from March 2018 to May 2021 were randomized into the control group and the research group by the random number table method, with 30 cases in each group. The control group received rbFGF plus limbal stem cell transplantation. The research group was treated with acupoint massage additionally.

Results: The total clinical effective rate in the study group was higher than that in the control group ( < 0.05); after treatment, the modulation transfer function (MTF) cutoff and Strehl ratio (SR) levels in the study group were significantly higher than those in the control group, and the tear film-related objective scatter index (TF-OSI) was significantly lower than that in the control group ( < 0.05); corneal horizontal curvature and corneal vertical curvature in the study group after treatment were significantly higher than those in the control group, and corneal astigmatism degree (CAD) levels were significantly lower than those in the control group ( < 0.05); the levels of the break-up time (BUT) and Schirmer test (SIT) in the study group were significantly higher after treatment, compared with the control group ( < 0.05); and the incidence of adverse manifestations in the study group was significantly lower than that in the control group ( < 0.05).

Conclusion: Acupoint massage combined with rbFGF ophthalmic gel combined with limbal stem cell transplantation is effective in clinical treatment of pterygium.
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http://dx.doi.org/10.1155/2022/7103496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213128PMC
June 2022

Tensor Recovery With Weighted Tensor Average Rank.

IEEE Trans Neural Netw Learn Syst 2022 Jun 22;PP. Epub 2022 Jun 22.

In this article, a curious phenomenon in the tensor recovery algorithm is considered: can the same recovered results be obtained when the observation tensors in the algorithm are transposed in different ways? If not, it is reasonable to imagine that some information within the data will be lost for the case of observation tensors under certain transpose operators. To solve this problem, a new tensor rank called weighted tensor average rank (WTAR) is proposed to learn the relationship between different resulting tensors by performing a series of transpose operators on an observation tensor. WTAR is applied to three-order tensor robust principal component analysis (TRPCA) to investigate its effectiveness. Meanwhile, to balance the effectiveness and solvability of the resulting model, a generalized model that involves the convex surrogate and a series of nonconvex surrogates are studied, and the corresponding worst case error bounds of the recovered tensor is given. Besides, a generalized tensor singular value thresholding (GTSVT) method and a generalized optimization algorithm based on GTSVT are proposed to solve the generalized model effectively. The experimental results indicate that the proposed method is effective.
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http://dx.doi.org/10.1109/TNNLS.2022.3182541DOI Listing
June 2022

Use of physical restraints and its associated risk factors in the aged care facilities: A multicentre cross-sectional study.

J Clin Nurs 2022 Jun 19. Epub 2022 Jun 19.

School of Nursing, Hunan University of Chinese Medicine, Changsha, China.

Aims And Objectives: To investigate the use of physical restraints in aged care facilities(ACFs)and analyse its associated risk factors.

Background: Physical restraints have been widely used in ACFs worldwide, but they can cause physical and mental harm to older people. It is important to regulate the use of physical restraint.

Design: A cross-sectional observational and correlational multicentre study.

Methods: By convenience sampling method, we selected eight ACFs in four representative regions of Hunan province, China, for this study. The ACF-related information was obtained by interviewing the managers and reviewing records. We conducted investigation and observation on the elderly in the ACFs to understand the use of physical restraints at three different times: 9:30-11:30, 16:00-18:00 and 19:30-21:30 on a working day. The STROBE checklist was followed for this cross-sectional study.

Results: This study found that the utilisation rate of physical restraints was 23.2%. The critical risk factors affecting the use of physical restrains include the following: (1) the ratio of nursing staff to the elderly residents; (2)whether there is a dementia care unit at the facility; (3) the number of elderly residents in each room; (4) the elderly residents' age, degree of education, marital status, care dependence and cognitive impairment; (5) whether the elderly has suffered from a stroke or senile dementia; (6) whether the elderly carries medical catheters.

Conclusion: There is a lack of standardisation in the use of physical restraints in ACFs of central China. Chinese ACFs should develop guidelines and reduction measures to standardise the use of physical restraints, basing on the key factors affecting the use of physical restraints.

Relevance To Clinical Practice: The use of physical restraints in ACFs is threatening the safety of the elderly residents. Understanding the implementation of physical restraint in ACFs can provide reference for reducing the use of physical restraint.
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http://dx.doi.org/10.1111/jocn.16406DOI Listing
June 2022

attenuated vascular remodeling by alleviating endoplasmic reticulum stress in spontaneously hypertensive rats.

Food Funct 2022 Jun 6;13(11):6293-6305. Epub 2022 Jun 6.

Department of Pharmacy, Lishui hospital of traditional Chinese medicine, Lishui, Zhejiang, 323000, China.

(CS), the leaves of S. Y. Hu., is an effective tea to prevent and treat hypertension in China. This study aimed to explore the effect and mechanism of CS in the protection against vascular remodeling in hypertension. Spontaneously hypertensive rats (SHRs) were orally administered with aqueous extracts of CS for 6 months. The blood pressure and morphological changes of the aorta were measured. Their mechanisms were studied by combining chemical identification, network pharmacology analysis and validation . Hypertensive rats showed an impaired vascular structure and dyslipidemia as illustrated by the increase of the vascular media thickness and collagen deposition in the aorta. CS treatment exhibited significant beneficial effects on blood pressure control and aortal morphology. A total of 21 compounds from CS were identified, which were linked to 106 corresponding targeted genes for vascular remodeling. The network pharmacology predicted that CS prevented vascular remodeling through the endoplasmic reticulum stress pathway. The experiments further showed that CS treatment upregulated Glucose-Regulated Protein 78 and downregulated CCAAT-enhancer-binding protein homologous protein at both mRNA and protein levels, paralleling reduced apoptotic cells in the arterial wall. Additionally, CS diminished the low-density lipoprotein cholesterol levels, total cholesterol contents and triglyceride/high-density lipoprotein cholesterol ratios in the sera of SHRs, which might also contribute to its protection of vessels. Collectively, CS protects against vascular modeling by suppressing endoplasmic reticulum stress-related apoptosis in hypertension, and it could be a potential agent for the prevention and treatment of vascular modeling.
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http://dx.doi.org/10.1039/d1fo04381aDOI Listing
June 2022

TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferation.

Genome Res 2022 Jun 24;32(6):1026-1041. Epub 2022 May 24.

MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

Polypeptides encoded by long noncoding RNAs (lncRNAs) are a novel class of functional molecules. However, whether these hidden polypeptides participate in the TP53 pathway and play a significant biological role is still unclear. Here, we discover that TP53-regulated lncRNAs can encode peptides, two of which are functional in various human cell lines. Using ribosome profiling and RNA-seq approaches in HepG2 cells, we systematically identified more than 300 novel TP53-regulated lncRNAs and further confirmed that 15 of these TP53-regulated lncRNAs encode peptides. Furthermore, several peptides were validated by mass spectrometry. Ten of the novel translational lncRNAs are directly inducible by TP53 in response to DNA damage. We show that the TP53-inducible peptides TP53LC02 and TP53LC04, but not their lncRNAs, can suppress cell proliferation. TP53LC04 peptide also has a function associated with cell proliferation by regulating the cell cycle in response to DNA damage. This study shows that TP53-regulated lncRNAs can encode new functional peptides, leading to the expansion of the TP53 tumor-suppressor network and providing novel potential targets for cancer therapy.
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http://dx.doi.org/10.1101/gr.275831.121DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248879PMC
June 2022

Nomogram and Machine Learning Models Predict 1-Year Mortality Risk in Patients With Sepsis-Induced Cardiorenal Syndrome.

Front Med (Lausanne) 2022 29;9:792238. Epub 2022 Apr 29.

Department of Nephrology, School of Medicine, Tongji Hospital, Tongji University, Shanghai, China.

Objective: Early prediction of long-term outcomes in patients with sepsis-induced cardiorenal syndrome (CRS) remains a great challenge in clinical practice. Herein, we aimed to construct a nomogram and machine learning model for predicting the 1-year mortality risk in patients with sepsis-induced CRS.

Methods: This retrospective study enrolled 340 patients diagnosed with sepsis-induced CRS in Shanghai Tongji Hospital between January 2015 and May 2019, as a discovery cohort. Two predictive models, the nomogram and machine learning model, were used to predict 1-year mortality. The prognostic variables used to develop the nomogram were identified based on a forward stepwise binary logistic regression, and the predictive ability of the nomogram was evaluated by the areas under the receiver operating characteristic curve (AUC) and the calibration curve. Meanwhile, machine learning (ML) techniques, such as support vector machine, random forest (RF), and gradient boosted decision tree, were assessed mainly by accuracy and AUC. Feature ranking analysis was performed using the ML algorithm. Both nomogram and ML models were externally validated by an independent cohort of 103 patients diagnosed with sepsis-induced CRS between June 2019 and December 2020.

Results: Age, sequential sepsis-related organ failure score (SOFA), serum myoglobin (MYO), vasopressor use, and mechanical ventilation were identified as independent risk factors for 1-year mortality in the nomogram predictive model. In the discovery cohort, the nomogram yielded higher AUC for predicting mortality than did the SOFA score (0.855 [95% CI: 0.815-0.895] vs. 0.756 [95% CI: 0.705-0.808]). For ML, the model developed by RF showed the highest accuracy (0.765) and AUC (0.854). In feature ranking analysis, factors such as age, MYO, SOFA score, vasopressor use, and baseline serum creatinine were identified as important features affecting 1-year prognosis. Moreover, the nomogram and RF model both performed well in external validation, with an AUC of 0.877 and 0.863, respectively.

Conclusion: Our nomogram and ML models showed that age, SOFA score, serum MYO levels, and the use of vasopressors during hospitalization were the main factors influencing the risk of long-term mortality. Our models may serve as useful tools for assessing long-term prognosis in patients with sepsis-induced CRS.
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http://dx.doi.org/10.3389/fmed.2022.792238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9099150PMC
April 2022

Whole Genome Profiling of Lung Microbiome in Solid Organ Transplant Recipients Reveals Virus Involved Microecology May Worsen Prognosis.

Front Cell Infect Microbiol 2022 16;12:863399. Epub 2022 Mar 16.

Department of Critical Care Medicine, West China Hospital, West China Clinical Medical School, Sichuan University, Chengdu, China.

Solid organ transplantation (SOT) is the final therapeutic option for recipients with end-stage organ failure, and its long-term success is limited by infections and chronic allograft dysfunction. Viral infection in SOT recipients is considered an important factor affecting prognosis. In this study, we retrospectively analyzed 43 cases of respiratory infections in SOT recipients using metagenomic next-generation sequencing (mNGS) for bronchoalveolar lavage fluid (BALF). At least one virus was detected in 26 (60.5%) recipients, while 17 (39.5%) were virus-negative. Among virus-positive recipients, cytomegalovirus (CMV) was detected in 14 (32.6%), Torque teno virus (TTV) was detected in 9 (20.9%), and other viruses were detected in 6 (14.0%). Prognostic analysis showed that the mortality of the virus-positive group was higher than that of the virus-negative group regardless whether it is the main cause of infection. Analysis of different types of viruses showed that the mortality of the CMV-positive group was significantly higher than that of the CMV-negative group, but no significant difference was observed in other type of virus groups. The diversity analysis of the lung microbiome showed that there was a significant difference between the virus-positive group and the negative group, in particular, the significant differences in microorganisms such as (PJP) and were detected. Moreover, in the presence of CMV, , , and other species showed dramatic changes in the lung of SOT patients, implying that high degree of co-infection between CMV and . Taken together, our study shows that the presence of virus is associated with worse prognosis and dramatically altered lung microbiota in SOT recipients.
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http://dx.doi.org/10.3389/fcimb.2022.863399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8967177PMC
April 2022

Rapid detection of 5 fungal diseases in sunflower (Helianthus annuus) using dual priming oligonucleotide system-based multiplex PCR and capillary electrophoresis.

SLAS Technol 2022 08 30;27(4):253-260. Epub 2022 Mar 30.

Hubei Province Engineering Research Centre of Legume Plants, College of Life Sciences, Jianghan University, Wuhan, China. Electronic address:

Rapid and accurate diagnosis of fungal pathogens is essential for disease control in sunflower. In the present study, a multiplex PCR assay was developed based on the dual priming oligonucleotide (DPO) system, which was used to simultaneously detect and identify five major sunflower fungal pathogens. There was no cross-reactivity among the pathogens tested. In each reaction, 0.1 ng genomic DNA templates were sufficient to ensure specificity and accuracy. The system exhibited high adaptability over a wide range of annealing temperatures. No mismatch or nonspecific amplification was observed in the annealing temperatures tested. In combination with capillary electrophoresis, the DPO-primer-based multiplex PCR system provides a rapid, reliable and cost-efficient solution for the diagnosis of fungal pathogens in sunflower.
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http://dx.doi.org/10.1016/j.slast.2022.03.004DOI Listing
August 2022

Photoredox/Copper Dual-Catalyzed Benzylic C-H Esterification via Radical-Polar Crossover.

Org Lett 2022 04 31;24(14):2679-2683. Epub 2022 Mar 31.

College of Medicine, Jiaxing University, 118 Jiahang Road, Jiaxing 314001, P. R. China.

Herein, we report a distinctive photoredox/copper dual-catalyzed esterification of benzylic C-H bonds through the combination of photoredox-mediated hydrogen atom transfer and Cu(II)-mediated radical-polar crossover. This methodology demonstrates a high functional group tolerance (>40 examples) and moderate to good yields with structurally diverse benzylic C-H substrates. Notably, stoichiometric amounts of carboxylic acids are used as coupling partners, which allows the synthesis of structurally diverse benzylic esters and the late-stage functionalization of pharmaceuticals.
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http://dx.doi.org/10.1021/acs.orglett.2c00763DOI Listing
April 2022

SST: Spatial and Semantic Transformers for Multi-Label Image Recognition.

IEEE Trans Image Process 2022 21;31:2570-2583. Epub 2022 Mar 21.

Multi-label image recognition has attracted considerable research attention and achieved great success in recent years. Capturing label correlations is an effective manner to advance the performance of multi-label image recognition. Two types of label correlations were principally studied, i.e., the spatial and semantic correlations. However, in the literature, previous methods considered only either of them. In this work, inspired by the great success of Transformer, we propose a plug-and-play module, named the Spatial and Semantic Transformers (SST), to simultaneously capture spatial and semantic correlations in multi-label images. Our proposal is mainly comprised of two independent transformers, aiming to capture the spatial and semantic correlations respectively. Specifically, our Spatial Transformer is designed to model the correlations between features from different spatial positions, while the Semantic Transformer is leveraged to capture the co-existence of labels without manually defined rules. Other than methodological contributions, we also prove that spatial and semantic correlations complement each other and deserve to be leveraged simultaneously in multi-label image recognition. Benefitting from the Transformer's ability to capture long-range correlations, our method remarkably outperforms state-of-the-art methods on four popular multi-label benchmark datasets. In addition, extensive ablation studies and visualizations are provided to validate the essential components of our method.
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http://dx.doi.org/10.1109/TIP.2022.3148867DOI Listing
March 2022

Correction: A mesoporous polydopamine nanoparticle enables highly efficient manganese encapsulation for enhanced MRI-guided photothermal therapy.

Nanoscale 2022 Mar 24;14(12):4770. Epub 2022 Mar 24.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Correction for 'A mesoporous polydopamine nanoparticle enables highly efficient manganese encapsulation for enhanced MRI-guided photothermal therapy' by Yan Wu , , 2021, , 6439-6446, DOI: D1NR00957E.
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http://dx.doi.org/10.1039/d1nr90265bDOI Listing
March 2022

Application of three-dimensional (3D) reconstruction and printing as an elective course for undergraduate medical students: an exploratory trial.

Authors:
Xiaoqin Zhang Yi Wu

Surg Radiol Anat 2022 04 18;44(4):497-498. Epub 2022 Feb 18.

Department of Digital Medicine, College of Biomedical Engineering and Medical Imaging, Army Medical University (Third Military Medical University), Chongqing, 400038, China.

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http://dx.doi.org/10.1007/s00276-022-02904-zDOI Listing
April 2022

Recent Progress on Bio-Based Polyesters Derived from 2,5-Furandicarbonxylic Acid (FDCA).

Polymers (Basel) 2022 02 6;14(3). Epub 2022 Feb 6.

Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, 1219 Zhongguan West Road, Zhenhai District, Ningbo 315201, China.

The big challenge today is the upgrading of sustainable materials to replace miscellaneous ones from petroleum resources. Thus, a generic bio-based building block lays the foundation of the huge bio-market to green economy. 2,5-Furandicarboxylic acid (FDCA), a rigid diacid derived from lignocellulose or fructose, represents a great potential as a contender to terephthalic acid (TPA). Recently, studies on the synthesis, modification, and functionalization of bio-based polyesters based on FDCA have attracted widespread attention. To apply furanic polyesters on engineering plastics, packaging materials, electronics, etc., researchers have extended the properties of basic FDCA-based homo-polyesters by directional copolymerization and composite preparation. This review covers the synthesis and performance of polyesters and composites based on FDCA with emphasis bedded on the thermomechanical, crystallization, barrier properties, and biodegradability. Finally, a summary of what has been achieved and the issues waiting to be addressed of FDCA-based polyester materials are suggested.
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http://dx.doi.org/10.3390/polym14030625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8838965PMC
February 2022

Siamese Implicit Region Proposal Network With Compound Attention for Visual Tracking.

IEEE Trans Image Process 2022 16;31:1882-1894. Epub 2022 Feb 16.

Recently, siamese-based trackers have achieved significant successes. However, those trackers are restricted by the difficulty of learning consistent feature representation with the object. To address the above challenge, this paper proposes a novel siamese implicit region proposal network with compound attention for visual tracking. First, an implicit region proposal (IRP) module is designed by combining a novel pixel-wise correlation method. This module can aggregate feature information of different regions that are similar to the pre-defined anchor boxes in Region Proposal Network. To this end, the adaptive feature receptive fields then can be obtained by linear fusion of features from different regions. Second, a compound attention module including a channel and non-local attention is raised to assist the IRP module to perform a better perception of the scale and shape of the object. The channel attention is applied for mining the discriminative information of the object to handle the background clutters of the template, while non-local attention is trained to aggregate the contextual information to learn the semantic range of the object. Finally, experimental results demonstrate that the proposed tracker achieves state-of-the-art performance on six challenging benchmark tests, including VOT-2018, VOT-2019, OTB-100, GOT-10k, LaSOT, and TrackingNet. Further, our obtained results demonstrate that the proposed approach can be run at an average speed of 72 FPS in real time.
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http://dx.doi.org/10.1109/TIP.2022.3148876DOI Listing
February 2022

Abnormal Iron and Lipid Metabolism Mediated Ferroptosis in Kidney Diseases and Its Therapeutic Potential.

Metabolites 2022 Jan 10;12(1). Epub 2022 Jan 10.

Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA.

Ferroptosis is a newly identified form of regulated cell death driven by iron-dependent phospholipid peroxidation and oxidative stress. Ferroptosis has distinct biological and morphology characteristics, such as shrunken mitochondria when compared to other known regulated cell deaths. The regulation of ferroptosis includes different molecular mechanisms and multiple cellular metabolic pathways, including glutathione/glutathione peroxidase 4(GPX4) signaling pathways, which are involved in the amino acid metabolism and the activation of GPX4; iron metabolic signaling pathways, which are involved in the regulation of iron import/export and the storage/release of intracellular iron through iron-regulatory proteins (IRPs), and lipid metabolic signaling pathways, which are involved in the metabolism of unsaturated fatty acids in cell membranes. Ferroptosis plays an essential role in the pathology of various kidneys diseases, including acute kidney injury (AKI), chronic kidney disease (CKD), autosomal dominant polycystic kidney disease (ADPKD), and renal cell carcinoma (RCC). Targeting ferroptosis with its inducers/initiators and inhibitors can modulate the progression of kidney diseases in animal models. In this review, we discuss the characteristics of ferroptosis and the ferroptosis-based mechanisms, highlighting the potential role of the main ferroptosis-associated metabolic pathways in the treatment and prevention of various kidney diseases.
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http://dx.doi.org/10.3390/metabo12010058DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8779729PMC
January 2022

Elevated lncRNA-UCA1 upregulates EZH2 to promote inflammatory response in sepsis-induced pneumonia via inhibiting HOXA1.

Carcinogenesis 2022 05;43(4):371-381

Department of Intensive Care Unit, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Sepsis is characterized by a dysregulated inflammatory response. We aimed to explore the role of the long noncoding RNA urothelial carcinoma associated 1 (lncRNA UCA1)/enhancer of zeste homolog 2 (EZH2)/homeobox A1 (HOXA1) axis in sepsis-induced pneumonia. The sepsis rat models and RLE-6TN cellular sepsis-induced pneumonia models were established using ligation and puncture (CLP) and lipopolysaccharide (LPS). The expression of UCA1, EZH2, and HOXA1 in rat lung tissues and RLE-6TN cells was detected. Then, the CLP rats were respectively treated with lentivirus to upregulate or downregulate the expression of UCA1 and EZH2 to measure their roles in the pathology, apoptosis, inflammation and phosphorylated NF-κB p65(p-p65) levels in CLP rat lung tissues. UCA1 and EZH2 expression was upregulated or downregulated in LPS-induced RLE-6TN cells to explore their effects on cell viability, apoptosis, inflammation and p-p65 levels. The interactions among UCA1, EZH2, and HOXA1 were identified. UCA1 and EZH2 were upregulated whereas HOXA1 was downregulated in CLP rat lung tissues and LPS-induced RLE-6TN cells. Elevated UCA1 or increased EZH2 aggravated pathology and promoted apoptosis, inflammation and phosphorylated NF-κB p-65 levels in CLP rat lung tissues, and inhibited viability while facilitated apoptosis, inflammation and phosphorylated NF-κB p-65 levels in LPS-induced RLE-6TN cells. Silenced EZH2 reversed the effects of UCA1 elevation on sepsis-induced pneumonia. UCA1 suppressed HOXA1 expression through physically interacting with EZH2. UCA1 overexpression upregulates EZH2 to repress HOXA1 expression, thus aggravating the progression of sepsis-induced pneumonia, which could be alleviated by EZH2 inhibition.
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http://dx.doi.org/10.1093/carcin/bgac004DOI Listing
May 2022

Effect of allisartan on blood pressure and left ventricular hypertrophy through Kv1.5 channels in hypertensive rats.

Clin Exp Hypertens 2022 Apr 11;44(3):199-207. Epub 2022 Jan 11.

Department of Cardiology, Southern Medical University Affiliated FengXian Hospital, Shanghai, China.

Background: The objective of the present work was to study the anti-hypertensive effect of allisartan on blood pressure (BP) and in facilitating left ventricular remodeling through voltage-gated potassium channels (Kv) 1.5 channels.

Methods: A total of 30 SD rats were randomly divided into sham operation group, hypertension control group, and allisartan treatment group. Hypertension was induced by renal artery stenosis. The animals of treatment group were administered with allisartan once a day at a dose of 30 mg/kg body weight through an oral gavage for 4 weeks. The heart function of animals post 4 weeks of treatment was evaluated by echocardiography, and the degree of ventricular hypertrophy and cardiomyocyte hypertrophy were evaluated by histomorphology. The expression of Kv1.5 is detected by real-time quantitative polymerase chain reaction while Western blotting was used to detect the protein expression.

Results: Four weeks after renal artery stenosis, a significant difference was observed in the whole heart ratio, left heart ratio, and cardiomyocyte area between allisartan treatment group and the hypertension control group (< .01). A significant decrease in BP of allisartan treatment group compared to hypertension control group (< .01) was observed. The expression of Kv1.5 mRNA was increased significantly (< .01) in allisartan treatment group compared to hypertension control group. Western blot analysis also confirmed the increased expression of Kv1.5 channel.

Conclusion: The results showed that allisartan lowers BP and improves left ventricular remodeling through increased expression of Kv1.5 mRNA.
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http://dx.doi.org/10.1080/10641963.2021.2018597DOI Listing
April 2022

Semantics-Guided Contrastive Network for Zero-Shot Object detection.

IEEE Trans Pattern Anal Mach Intell 2022 Jan 4;PP. Epub 2022 Jan 4.

Zero-shot object detection (ZSD), the task that extends conventional detection models to detecting objects from unseen categories, has emerged as a new challenge in computer vision. Most existing approaches on ZSD are based on a strict mapping-transfer strategy that learns a mapping function from visual to semantic space over seen categories, then directly generalizes the learned mapping function to unseen object detection. However, the ZSD task still remains challenging, since those works fail to consider the two key factors that hamper the ZSD performance: (a) the domain shift problem between seen and unseen classes leads to poor transferable ability of the model; (b) the original visual feature space is suboptimal for ZSD since it lacks discriminative information.To alleviate these issues, we develop a novel Semantics-Guided Contrastive Network for ZSD (ContrastZSD), a detection framework that first brings the contrastive learning paradigm into the realm of ZSD. The pairwise contrastive tasks take advantage of class label and semantic relation as additional supervision signals. Under the guidance of those explicit semantic supervision, the model can learn more knowledge about unseen categories to avoid over-fitting to the seen concepts.
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http://dx.doi.org/10.1109/TPAMI.2021.3140070DOI Listing
January 2022

Targeting lysine-specific demethylase 1A inhibits renal epithelial-mesenchymal transition and attenuates renal fibrosis.

FASEB J 2022 01;36(1):e22122

Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.

Lysine-specific histone demethylase 1 (LSD1) as the first identified histone/lysine demethylase regulates gene expression and protein functions in diverse diseases. In this study, we show that the expression of LSD1 is increased in mouse kidneys with unilateral ureteral obstruction (UUO) and in cultured NRK-52E cells undergoing TGF-β1-induced epithelial-mesenchymal transition (EMT). Inhibition of LSD1 with its specific inhibitor ORY1001 attenuated renal EMT and fibrosis, which was associated with decreased the deposition of extracellular matrix proteins and the expression of fibrotic markers, including α-smooth muscle actin (α-SMA) and fibronectin, and the recovery of E-cadherin expression and decrease of N-cadherin expression in UUO kidneys and in NRK-52E cells induced with TGF-β1. Targeting LSD1 also decreased the expression of Snail family transcriptional repressor 1 (Snail-1) and its interaction with LSD1 in UUO kidneys and in NRK-52E cells treated with TGF-β1. In addition, we identified a novel LSD1-14-3-3ζ-PKCα axis in the regulation of the activation of AKT and Stat3 and then the activation of fibroblasts. This study suggests that LSD1 plays a critical role in regulation of renal EMT and fibrosis through activation of diverse signaling pathways and places an emphasis that LSD1 has potential as a therapeutic target for the treatment of renal fibrosis.
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http://dx.doi.org/10.1096/fj.202101566RDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9154004PMC
January 2022

Enhancing Oil Recovery from Low-Permeability Reservoirs with a Thermoviscosifying Water-Soluble Polymer.

Molecules 2021 Dec 9;26(24). Epub 2021 Dec 9.

Polymer Research Institute, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.

Water-soluble polymers, mainly partially hydrolyzed polyacrylamide (HPAM), have been used in the enhanced oil recovery (EOR) process. However, the poor salt tolerance, weak thermal stability and unsatisfactory injectivity impede its use in low-permeability hostile oil reservoirs. Here, we examined the adaptivity of a thermoviscosifying polymer (TVP) in comparison with HPAM for chemical EOR under simulated conditions (45 °C, 4500 mg/L salinity containing 65 mg/L Ca and Mg) of low-permeability oil reservoirs in Daqing Oilfield. The results show that the viscosity of the 0.1% TVP solution can reach 48 mPa·s, six times that of HPAM. After 90 days of thermal aging at 45 °C, the TVP solution had 71% viscosity retention, 18% higher than that of the HPAM solution. While both polymer solutions could smoothly propagate in porous media, with permeability of around 100 milliDarcy, TVP exhibited stronger mobility reduction and permeability reduction than HPAM. After 0.7 pore volume of 0.1% polymer solution was injected, TVP achieved an incremental oil recovery factor of 13.64% after water flooding, 3.54% higher than that of HPAM under identical conditions. All these results demonstrate that TVP has great potential to be used in low-permeability oil reservoirs for chemical EOR.
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http://dx.doi.org/10.3390/molecules26247468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709243PMC
December 2021

Cytisine-like alkaloids from the seeds of Hemsl. et Wils.

Nat Prod Res 2021 Dec 22:1-7. Epub 2021 Dec 22.

College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, People's Republic of China.

Two new cytisine-like alkaloids, hositisines C () and D (), were isolated from the seeds of along with four known compounds, (-)-tinctorine (), -adenosine (), 2'-deoxyadenosine (), and 7, 2', 4'-trihydroxy-5-methoxyisoflavanone (). Their structures were established using extensive spectroscopic techniques (UV, IR, CD, HRESIMS, 1 D and 2 D NMR). In the cytotoxic activity, compounds - and 5-fluorouracil (positive control) displayed inhibitory effects against HepG2 cells, exhibiting IC values of 44.52 ± 7.83 μM, 111.49 ± 12.76 μM, 127.72 ± 18.67 μM, and 16.37 ± 3.82 μM.
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http://dx.doi.org/10.1080/14786419.2021.2005591DOI Listing
December 2021

Deficits in N-Methyl-D-Aspartate Receptor Function and Synaptic Plasticity in Hippocampal CA1 in APP/PS1 Mouse Model of Alzheimer's Disease.

Front Aging Neurosci 2021 30;13:772980. Epub 2021 Nov 30.

Zhejiang Key Laboratory of Pathophysiology, Department of Pharmacology, School of Medicine, Ningbo University, Ningbo, China.

The N-methyl-D-aspartate receptor is a critical molecule for synaptic plasticity and cognitive function. Impaired synaptic plasticity is thought to contribute to the cognitive impairment associated with Alzheimer's disease (AD). However, the neuropathophysiological alterations of N-methyl-D-aspartate receptor (NMDAR) function and synaptic plasticity in hippocampal CA1 in transgenic rodent models of AD are still unclear. In the present study, APP/PS1 mice were utilized as a transgenic model of AD, which exhibited progressive cognitive impairment including defective working memory, recognition memory, and spatial memory starting at 6 months of age and more severe by 8 months of age. We found an impaired long-term potentiation (LTP) and reduced NMDAR-mediated spontaneous excitatory postsynaptic currents (sEPSCs) in the hippocampal CA1 of APP/PS1 mice with 8 months of age. Golgi staining revealed that dendrites of pyramidal neurons had shorter length, fewer intersections, and lower spine density in APP/PS1 mice compared to control mice. Further, the reduced expression levels of NMDAR subunits, PSD95 and SNAP25 were observed in the hippocampus of APP/PS1 mice. These results suggest that NMDAR dysfunction, impaired synaptic plasticity, and disrupted neuronal morphology constitute an important part of the neuropathophysiological alterations associated with cognitive impairment in APP/PS1 mice.
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http://dx.doi.org/10.3389/fnagi.2021.772980DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669806PMC
November 2021

Modulating adult neurogenesis affects synaptic plasticity and cognitive functions in mouse models of Alzheimer's disease.

Stem Cell Reports 2021 12 2;16(12):3005-3019. Epub 2021 Dec 2.

Department of Neurobiology and Department of Neurology of the First Affiliated Hospital, Zhejiang University School of Medicine, NHC and CAMS Key Laboratory of Medical Neurobiology, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou, Zhejiang Province 310058, China. Electronic address:

New neurons are abnormal in the adult hippocampus of Alzheimer's disease (AD) mouse models. The effects of modulating adult neurogenesis on AD pathogenesis differ from study to study. We reported recently that ablation of adult neural stem cells (aNSCs) was associated with improved memory in AD models. Here, we found that long-term potentiation (LTP) was improved in the hippocampus of APP/PS1 mice after ablation of aNSCs. This effect was confirmed in hAPP-J20 mice, a second AD mouse model. On the other hand, we found that exposure to enriched environment (EE) dramatically increased the number of DCX neurons, promoted dendritic growth, and affected the location of newborn neurons in the dentate gyrus of APP/PS1 mice, and EE exposure significantly ameliorated memory deficits in APP/PS1 mice. Together, our data suggest that both inhibiting abnormal adult neurogenesis and enhancing healthy adult neurogenesis could be beneficial for AD, and they are not mutually exclusive.
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http://dx.doi.org/10.1016/j.stemcr.2021.11.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8693766PMC
December 2021

A new force-measuring balance for large-scale model in hypersonic wind tunnel.

Rev Sci Instrum 2021 Nov;92(11):115005

School of Mechanical Engineering, SouthWest Jiaotong University, Chengdu 610031, People's Republic of China.

There is a strong coupling effect between the body and propulsion system of the integrated hypersonic vehicle. The scaled-down model cannot reflect the laws of physics. Therefore, carrying out hypersonic aerodynamic tests using large-scale aircraft models is an urgent difficulty and the key link in the research of hypersonic aircraft. This Note presents a design scheme of large-scale and heavy-load three-component gauge strain balance. The balance innovatively uses the design of a "herringbone" force measuring element and a support plate to ensure the sensitivity of the main channel and solve the problem of serious coupling between channels. In order to solve the problem of insufficient stiffness caused by the large scale of the model, two convex plates are added to the contact surface between the model and the balance. Therefore, the difficulties encountered by large-scale aircraft models in a pulsed wind tunnel can be overcome effectively. The balance is verified by static calibration and wind tunnel test to meet the requirements. The results show that the balance meets the requirements of the aerodynamic load test of the large-scale and heavy-duty hypersonic vehicle model in a pulse combustion wind tunnel.
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http://dx.doi.org/10.1063/5.0054090DOI Listing
November 2021

Semantic-Aware Dehazing Network With Adaptive Feature Fusion.

IEEE Trans Cybern 2021 Nov 19;PP. Epub 2021 Nov 19.

Despite that convolutional neural networks (CNNs) have shown high-quality reconstruction for single image dehazing, recovering natural and realistic dehazed results remains a challenging problem due to semantic confusion in the hazy scene. In this article, we show that it is possible to recover textures faithfully by incorporating semantic prior into dehazing network since objects in haze-free images tend to show certain shapes, textures, and colors. We propose a semantic-aware dehazing network (SDNet) in which the semantic prior is taken as a color constraint for dehazing, benefiting the acquisition of a reasonable scene configuration. In addition, we design a densely connected block to capture global and local information for dehazing and semantic prior estimation. To eliminate the unnatural appearance of some objects, we propose to fuse the features from shallow and deep layers adaptively. Experimental results demonstrate that our proposed model performs favorably against the state-of-the-art single image dehazing approaches.
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http://dx.doi.org/10.1109/TCYB.2021.3124231DOI Listing
November 2021

Ferroptosis Promotes Cyst Growth in Autosomal Dominant Polycystic Kidney Disease Mouse Models.

J Am Soc Nephrol 2021 11;32(11):2759-2776

Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota

Background: Autosomal dominant polycystic kidney disease (ADPKD), the most common inherited kidney disease, is regulated by different forms of cell death, including apoptosis and autophagy. However, the role in ADPKD of ferroptosis, a recently discovered form of cell death mediated by iron and lipid metabolism, remains elusive.

Methods: To determine a pathophysiologic role of ferroptosis in ADPKD, we investigated whether the absence of (encoding polycystin-1) affected the expression of key factors involved in the process of ferroptosis, using Western blot and qRT-PCR analysis in mutant renal cells and tissues. We also examined whether treatment with erastin, a ferroptosis inducer, and ferrostain-1, a ferroptosis inhibitor, affected cyst growth in mutant mouse models.

Results: We found that kidney cells and tissues lacking exhibit extensive metabolic abnormalities, including reduced expression of the system Xc amino acid antiporter (critical for import of cystine), of iron exporter (ferroportin), and of GPX4 (a key and negative regulator of ferroptosis). The abnormalities also include increased expression of iron importers (TfR1, DMT1) and HO-1, which in turn result in high iron levels, low GSH and GPX4 activity, increased lipid peroxidation, and propensity to ferroptosis. We further found that erastin increased, and ferrostatin-1 inhibited ferroptotic cell death and proliferation of -deficient cells in kidneys from mutant mice. A lipid peroxidation product increased in -deficient cells, 4HNE, promoted the proliferation of survived mutant cells activation of Akt, S6, Stat3, and Rb during the ferroptotic process, contributing to cyst growth.

Conclusion: These findings indicate that ferroptosis contributes to ADPKD progression and management of ferroptosis may be a novel strategy for ADPKD treatment.
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http://dx.doi.org/10.1681/ASN.2021040460DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8806097PMC
November 2021
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