Publications by authors named "Xiaoqiang Wu"

29 Publications

  • Page 1 of 1

A self-alignment method for gravitational apparent acceleration identification and accelerometer bias estimation based on repeated navigation solution.

Rev Sci Instrum 2021 Jun;92(6):064505

T-SEA Marine Technology Co., Ltd., Zhangjiagang 215600, China.

Estimating inertial measurement unit error without any external reference information has always been a difficult problem. The method based on the reconstruction of the gravitational apparent motion in the inertial frame can estimate the accelerometer bias, but a long identification time is needed to get high alignment accuracy. In order to reduce the convergence time and improve the accuracy of estimation, a fast estimation method based on repetitive navigation under the excitation of swing motion is proposed. Two data processing methods, the forward-forward loop and the backward-forward loop, are studied and compared in this paper. In addition, the influence of data length on the experiment result was analyzed. The simulation and experimental results show that the forward-forward loop calculation cannot solve the contradiction between speed and accuracy, while the backward-forward loop calculation can effectively estimate the accelerometer bias and realize self-alignment, with a short alignment time and high alignment accuracy.
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http://dx.doi.org/10.1063/5.0050368DOI Listing
June 2021

Fecal Microbiota Transplantation as an Effective Treatment for Carbapenem-Resistant Infection in a Renal Transplant Patient.

Infect Drug Resist 2021 14;14:1805-1811. Epub 2021 May 14.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, People's Republic of China.

Background: In renal transplant recipients, carbapenem-resistant (CRKP) infection is a common complication, and usually associated with severe clinical outcomes due to a lack of effective treatment.

Case Presentation: A 37-year-old woman with CRKP infection one month after kidney transplantation was involved in this study. Microbial characteristics of fecal samples from the patient were analyzed. Fecal microbiota transplantation (FMT) was performed for treating the CRKP infection. One week after FMT, the patient's urine and anal swab cultures returned negative for CRKP, and 17 days after FMT, the incision showed complete healing. Moreover, the patient had no symptoms of infection two months after FMT. Alpha diversity analyses showed that before FMT, the patient was associated with obviously lower species richness and diversity than the donor, which significantly increased at one week, three weeks and two months after FMT. Beta diversity analyses showed that though the patient's microbial community post-FMT still differed from the donor composition, their distances decreased visibly, especially at one week and three weeks after FMT. Obvious shift in microbial composition could be observed before and after FMT. The microbial composition of the patient post FMT resembled the donor composition. Relative abundance of genera such as and increased after FMT, while the relative abundance of significantly decreased.

Conclusion: This study demonstrated the therapeutic effect of FMT on infections caused by CRKP for a renal transplant patient. Further studies are required to confirm the findings of this study.
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http://dx.doi.org/10.2147/IDR.S308308DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131010PMC
May 2021

Numerical study of structural parameters influencing flow in a lunular valveless pump.

Rev Sci Instrum 2021 Feb;92(2):025009

Institute of Precision Machinery, Zhejiang Normal University, Jinhua 321004, China.

Piezoelectric pumps are widely applied in medical transportation, bioengineering, and mechanical lubrication. However, the backflow phenomenon of the valveless piezoelectric pump is more prominent. Therefore, this paper designed a lunular piezoelectric pump to reduce the backflow. The three-dimensional flow field of the piezoelectric pump was analyzed using ANSYS Computational Fluid X. Based on the numerical results, the design of the lunular piezoelectric pump was improved, and a new model of the piezoelectric pump with better performance was developed. A prototype was made, and an experimental verification was carried out. The experimental results show that the maximum flow rate per unit area is 404.812 µl/min and maximum back pressure is 22.3 cmHO, which achieves the optimal performance of the pump and verifies the rationality of Computational Fluid X simulation. Thus, the performance of a pressure pump with low driving frequency can be predicted by Computational Fluid X simulation, and the structural optimization method is reliable. This research can help to broaden the application of the piezoelectric pump.
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http://dx.doi.org/10.1063/5.0029298DOI Listing
February 2021

Gut microbiota alterations associated with antibody-mediated rejection after kidney transplantation.

Appl Microbiol Biotechnol 2021 Mar 24;105(6):2473-2484. Epub 2021 Feb 24.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, 450003, China.

Antibody-mediated rejection (AMR) has become the major challenge for kidney transplantation, and the efficacy of existing therapies was limited to prevent AMR. Increasing evidences have demonstrated the link between gut microbiota alterations and allograft outcome. However, there has been no comprehensive analysis to profile the gut microbiota associated with AMR after kidney transplantation. We performed this study to characterize the gut microbiota possibly associated with AMR. Fecal specimens were collected from 24 kidney transplantation recipients with AMR and 29 controls. DNA extracted from the specimens was processed for 16S rRNA gene sequencing using Illumina MiSeq. Gut microbial community of recipients with AMR was significantly different from that of controls based on unweighted (P = 0.001) and weighted (P = 0.02) UniFrac distances, and the bacterial richness (observed species: P = 0.0448; Chao1 index: P = 0.0450; ACE index: P = 0.0331) significantly decreased in the AMR group. LEfSe showed that 1 phylum, 5 classes, 7 families, and 10 genera were increased, whereas 1 class, 2 order, 3 families, and 4 genera were decreased in the AMR group. Specific taxa such as Clostridiales could be potentially used as biomarkers to distinguish the recipients with AMR from the controls (AUC = 0.77). PICRUSt analysis illustrated that 16 functional pathways were with significantly different abundances in the AMR and control groups. Our findings provide a foundation for further investigation on the role of gut microbiota in AMR after kidney transplantation, and potentially support novel diagnostic biomarkers and therapeutic options for AMR. KEY POINTS: • Gut microbial community of kidney recipients with AMR was different from that of controls. • Clostridiales is a potential marker to distinguish recipients with AMR from controls.
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http://dx.doi.org/10.1007/s00253-020-11069-xDOI Listing
March 2021

Uncovering the microbiota in renal cell carcinoma tissue using 16S rRNA gene sequencing.

J Cancer Res Clin Oncol 2021 Feb 21;147(2):481-491. Epub 2020 Nov 21.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, 450003, China.

Introduction: Increasing evidence indicates an important role of microbiota in cancer development and progression, while little is known about the correlation between microbiota and renal cell carcinoma (RCC). Thus, we performed this study to profile the intratumoral microbiota possibly associated with RCC.

Materials And Methods: Paired RCC and adjacent normal tissue samples were collected from 24 patients with RCC. V3-V4 variable region of microbial 16S rRNA gene was sequenced using Illumina MiSeq. Sequencing reads were processed using QIIME. Differentially abundant bacterial taxa between groups were identified by LEfSe, and their potential functions were inferred by PICRUSt.

Results: Decreased species diversity was presented in RCC tissues (Simpson index, P = 0.0340), and the composition of the bacterial community in RCC tissues was significantly distinct from that in normal tissues (unweighted UniFrac distance, P = 0.026; weighted UniFrac distance, P = 0.017). Compared with normal tissues, 25 taxa increased and 47 reduced taxa were identified in RCC tissues. Among these taxa, the class Chloroplast (AUC = 0.91, P < 0.0001) and the order Streptophyta (AUC = 0.89, P < 0.0001) showed high indication accuracy to discriminate RCC tissues from normal tissues. Furthermore, nine altered pathways were identified in RCC tissues to reveal the potential microbial function.

Conclusions: Our results have uncovered the presence of distinct microbiota in RCC and adjacent normal tissues and provided a better understanding of the possible role of the intratumoral microbiota in RCC. Further studies are required to confirm our results and determine the real correlation between microbiota and RCC.
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http://dx.doi.org/10.1007/s00432-020-03462-wDOI Listing
February 2021

Catalpol inhibits the proliferation, migration and metastasis of HCC cells by regulating miR‑140‑5p expression.

Mol Med Rep 2021 01 12;23(1). Epub 2020 Nov 12.

Department of Hepatobiliary Surgery, The People's Hospital of Xinchang, Shaoxing, Zhejiang 312500, P.R. China.

Hepatocellular carcinoma (HCC) is a frequent malignant tumor. Catalpol is a Chinese medicine extract with a number of pharmacologically active properties. The present study aimed to investigate the effects and mechanisms of catalpol in HCC. HCC cells were treated with catalpol in the presence or absence of microRNA (miR)‑140‑5p inhibitor, and assays to determine cell viability, proliferation, invasion and migration were performed. Reverse transcription‑quantitative PCR and western blotting were performed to determine the mRNA and protein expression levels of miR‑140‑5p, vimentin, N‑Cadherin and E‑Cadherin. Moreover, cells were treated with catalpol in the absence or presence of transforming growth factor (TGF)‑β1, and the cell morphology was observed under a microscope. The results demonstrated that catalpol inhibited cell proliferation, invasion and migration, and decreased the expression levels of vimentin and N‑cadherin, but increased the expression levels of E‑cadherin and miR‑140‑5p. Catalpol inhibited morphological changes in epithelial‑mesenchymal transformation (EMT) of cells induced by TGF‑β1. Following inhibition of miR‑140‑5p expression, the proliferation, invasion and migration of HCC cells were promoted, E‑cadherin expression was decreased, and the levels of vimentin and N‑cadherin were increased. The miR‑140‑5p inhibitor effectively reversed the inhibitory effect of catalpol on cell proliferation, invasion and migration. Thus, the results suggested that the antitumor potential of catalpol in HCC may be exerted by regulating the expression of miR‑140‑5p to inhibit proliferation, invasion, migration and EMT of HCC cells.
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http://dx.doi.org/10.3892/mmr.2020.11667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673346PMC
January 2021

What is the optimum systemic treatment for advanced/metastatic renal cell carcinoma of favourable, intermediate and poor risk, respectively? A systematic review and network meta-analysis.

BMJ Open 2020 08 27;10(8):e034626. Epub 2020 Aug 27.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, China

Purpose: The optimum systemic therapies for advanced/metastatic renal cell carcinoma (RCC) of favourable, intermediate and poor risk have not been established. We aimed to compare and rank the effects associated with systemic therapies in the first-line setting.

Methods: We searched PubMed, Cochrane databases, Web of Science and ClinicalTrials.gov for randomised controlled trials (RCT) published up to February 2020 of all available treatments for advanced/metastatic RCC. Analysis was done on a Bayesian framework.

Results: 15 unique RCTs including 8995 patients were identified. For advanced/metastatic RCC of favourable risk, avelumab plus axitinib was associated with a significantly higher improvement in progression-free survival (PFS) than sunitinib (HR 0.57, 95% CI 0.34 to 0.96). For intermediate-risk patients, cabozantinib, nivolumab plus ipilimumab, pembrolizumab plus axitinib and avelumab plus axitinib were associated with significantly higher improvement in PFS than sunitinib (HR 0.63, 95% CI 0.44 to 0.97; HR 0.66, 95% CI 0.53 to 0.81; HR 0.58, 95% CI 0.44 to 0.80; HR 0.62, 95% CI 0.47 to 0.83, respectively); pembrolizumab plus axitinib and nivolumab plus ipilimumab were associated with significantly higher improvement in overall survival (OS) than sunitinib (HR 0.53, 95% CI 0.34 to 0.81; HR 0.66, 95% CI 0.50 to 0.87, respectively). For poor-risk patients, nivolumab plus ipilimumab and pembrolizumab plus axitinib were associated with significantly higher improvement in PFS than sunitinib (HR 0.57, 95% CI 0.43 to 0.76; HR 0.48, 95% CI 0.30 to 0.82, respectively); nivolumab plus ipilimumab and pembrolizumab plus axitinib were significantly more efficacious for OS than sunitinib (HR 0.57, 95% CI 0.39 to 0.883; HR 0.43, 95% CI 0.23 to 0.80, respectively). For OS, there were 81% and 78% probabilities that pembrolizumab plus axitinib was the best option for intermediate-risk and poor-risk patients, respectively.

Conclusion: Avelumab plus axitinib might be the optimum treatment for advanced/metastatic RCC of favourable risk. Pembrolizumab plus axitinib might be the optimum treatment for intermediate-risk and poor-risk patients.
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http://dx.doi.org/10.1136/bmjopen-2019-034626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7454197PMC
August 2020

A novel Rauch-Tung-Streibel smoothing scheme based on the factor graph for autonomous underwater vehicles.

Rev Sci Instrum 2020 Jul;91(7):075109

School of Instrument Science and Engineering, Southeast University, Nanjing 210096, China.

In submarine surveying and mapping applications, a novel Rauch-Tung-Streibel smoothing (RTSS) scheme based on the factor graph for autonomous underwater vehicles is presented to gain a better offline navigation solution in this paper. The factor graph method is applied to optimally use observation information of multi-sensors with the asynchronous and short-term failure problems to overcome deficiencies of the federal Kalman filter in information fusion processing. Furthermore, the revised RTSS as a post-mission smoothing algorithm is performed by combining the results of the factor graph and one backward data processing through recursively updating the smoothed state and its covariance. From the simulation analysis, it is found that the factor graph mainly owns plug and play capability and contributes to the real-time navigation accuracy over the federal Kalman filtering. The RTSS provides better accuracy and smoothness for the position, velocity, and attitude at the same time compared to the corresponding real-time navigation solution, especially when signals are lost or sensors fail for a short time. With the best of both methods, a novel smoothing scheme combining the factor graph with the RTSS is built. Semi-physical experiment results verify the reliability and effectiveness of the proposed method.
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http://dx.doi.org/10.1063/5.0007538DOI Listing
July 2020

COVID-19 in a Kidney Transplant Patient.

Eur Urol 2020 06 6;77(6):769-770. Epub 2020 Apr 6.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.eururo.2020.03.036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271211PMC
June 2020

Microstructures of the spermatic cord with three-dimensional reconstruction of sections of the cord and application to varicocele.

Syst Biol Reprod Med 2020 Jun 23;66(3):216-222. Epub 2020 Mar 23.

Department of Urology, Zhujiang Hospital, Southern Medical University , Guangzhou, Guangdong Province, China.

The aim of the study was to investigate the micro-structures of the spermatic cord using histological examination with three-dimensional (3D) reconstruction of the serial tissue sections of the cord for clinical application in microscopic varicocelectomy. Human spermatic cord specimens obtained from 13 adult male cadavers were used to prepare serial transverse sections. The sections were stained to allow observation of the spermatic cord microstructures. The 3D reconstruction was performed with digitized serial sections by Mimics software. The microscopic varicocelectomy was performed based on the anatomical results of 3D reconstruction of the spermatic cord. The results showed the number of small spermatic veins, large spermatic veins, arteries, lymphatics or nerves were not markedly different between the subinguinal and inguinal regions or between the right and left sperm cord. The number of medium spermatic veins in the subinguinal region was obviously higher than at the inguinal level. The internal spermatic vessels and the vas deferens together with other associated vessels within the cremaster were separately enclosed by two thin and translucent sheaths, the internal spermatic fascia and the vas deferens fascia. We conclude that internal spermatic vessels and the vas deferens together with the associated neurovascular vessels are wrapped by two distinct sheaths separating them from the surrounding tissues. Microscopic varicocelectomy based on the anatomical results of 3D reconstruction of the spermatic cord is feasible.

Abbreviations: 3D: three-dimensional; ISF: internal spermatic fascia; ESF: external spermatic fascia; MHIV: High inguinal microsurgical varicocelectomy; MSIV: subinguinal microsurgical varicocelectomy; CAAD: computer-assisted anatomic dissection; HE: hematoxylin-eosin.
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http://dx.doi.org/10.1080/19396368.2020.1741723DOI Listing
June 2020

Recent Advances in the Synthesis, Surface Modifications and Applications of Core-Shell Magnetic Mesoporous Silica Nanospheres.

Chem Asian J 2020 Apr 18;15(8):1248-1265. Epub 2020 Mar 18.

College of Science, University of Shanghai for Science and Technology, No. 334 Jungong Road, Shanghai, 200093, P.R. China.

The hierarchically structured core-shell magnetic mesoporous silica nanospheres (Mag-MSNs) have attracted extensive attention, particularly in studies involving reliable preparations and diverse applications of the multifunctional nanomaterials in multi-disciplinary fields. Intriguingly, Mag-MSNs have been prepared with well-designed synthesis strategies and used as adsorbent materials, biomedicines, and in proteomics and catalysis due to their excellent magnetic responsiveness, enormous specific surface area and readiness for surface modifications. Through a carefully designed surface modification of Mag-MSNs, the performance and application prospects of the material are greatly improved. Typically, the introduction of various molecular matrices into the shell of Mag-MSNs facilitates the combination of surface modifications and magnetic separation technology. So far, as sustainable chemistry is concerned, it is important to recover the functionalized core-shell Mag-MSNs after the reaction and reuse them without losing activity. In this review, the design conceptions and the construction of core-shell Mag-MSNs are discussed. Furthermore, various surface modification approaches of core-shell Mag-MSNs are summarized, and recent applications of these functionalized nanomaterials in the fields of biomedicine, catalysis, proteomics and wastewater treatment are exemplified.
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http://dx.doi.org/10.1002/asia.202000045DOI Listing
April 2020

Role of immune checkpoint inhibitor-based therapies for metastatic renal cell carcinoma in the first-line setting: A Bayesian network analysis.

EBioMedicine 2019 Sep 19;47:78-88. Epub 2019 Aug 19.

Department of Urology, Henan Provincial People's Hospital; Zhengzhou University People's Hospital, Zhengzhou 450003, China. Electronic address:

Background: Several novel immune checkpoint inhibitor (ICI)-based treatments exhibited promising survival benefits for metastatic renal cell carcinoma (mRCC), yet there is no current guidance regarding the optimum first-line regimen. We performed this network analysis to compare the efficacy and safety of all available treatments for mRCC.

Methods: A systematic search of literature was conducted up to April 30, 2019, and the analysis was done on a Bayesian fixed-effect model.

Findings: Twenty-five randomized clinical trials (RCTs) involving 13,010 patients were included in this study. The results showed that for overall survival, pembrolizumab plus axitinib (hazard ratio [HR]: 0.53; 95% credible interval [CrI]: 0.38-0.73) and nivolumab plus ipilimumab (HR: 0.63; 95% CrI: 0.50-0.79) were significantly more effective than sunitinib, and pembrolizumab plus axitinib was probably (68%) to be the best choice. For progression-free survival, cabozantinib (HR: 0.66; 95% CrI: 0.46-0.94), pembrolizumab plus axitinib (HR: 0.69; 95% CrI: 0.57-0.84), avelumab plus axitinib (HR: 0.69; 95% CrI: 0.56-0.85), nivolumab plus ipilimumab (HR: 0.82; 95% CrI: 0.68-0.99), and atezolizumab plus bevacizumab (HR: 0.86; 95% CrI: 0.74-0.99) were statistically superior to sunitinib, and cabozantinib was likely (43%) to be the preferred options. Nivolumab plus ipilimumab (OR: 0.50; 95% CrI: 0.28-0.84), and atezolizumab plus bevacizumab (OR: 0.56; 95% CrI: 0.36-0.83) were associated with significantly lower rate of high-grade adverse events than sunitinib.

Interpretation: Our findings demonstrate that pembrolizumab plus axitinib might be the best treatment for mRCC, while nivolumab plus ipilimumab has the most favorable balance between efficacy and acceptability, and may provide new guidance to make treatment decisions. FUND: This research was supported by the Henan Provincial Scientific and Technological Research Project (Grant No. 192102310036).
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http://dx.doi.org/10.1016/j.ebiom.2019.08.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796578PMC
September 2019

Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing.

Biomed Res Int 2019 12;2019:1759697. Epub 2019 Jun 12.

Department of Urology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, China.

Delayed graft function (DGF) is one of the major obstacles for graft survival for kidney recipients. It is profound to reduce the incidence of DGF for maintaining long-term graft survival. However, the molecular regulation of DGF is still not adequately explained and the biomarkers for DGF are limited. Exosomes are cell-derived membrane vesicles, contents of which are stable and could be delivered into recipient cells to exert their biological functions. Consequently, exosome-derived proteomic and RNA signature profiles are often used to account for the molecular regulation of diseases or reflect the conditional state of their tissue as biomarkers. Few researches have been done to demonstrate the function of exosomes associated with DGF. In this study, high-throughput sequencing was used to explore the miRNA expression profiling of exosomes in the peripheral blood of kidney recipients with DGF. We identified 52 known and 5 conserved exosomal miRNAs specifically expressed in recipients with DGF. Three coexpressed miRNAs, hsa-miR-33a-5p_R-1, hsa-miR-98-5p, and hsa-miR-151a-5p, were observed to be significantly upregulated in kidney recipients with DGF. Moreover, hsa-miR-151a-5p was positively correlated with the first-week serum CR, BUN, and UA levels of the kidney recipients after transplantation. Furthermore, we also analyzed functions and signaling pathways of the three upregulated miRNAs target genes to uncover putative mechanism of how these exosomal miRNAs functioned in DGF. Overall, these findings identified biomarker candidates for DGF and provided new insights into the important role of the exosomal miRNAs regulation in DGF.
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http://dx.doi.org/10.1155/2019/1759697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594342PMC
December 2019

Effects of levosimendan on mortality in patients undergoing cardiac surgery: A systematic review and meta-analysis.

J Card Surg 2018 Jun 21;33(6):322-329. Epub 2018 May 21.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou, China.

Purpose: We sought to determine the impact of levosimendan on mortality following cardiac surgery based on large-scale randomized controlled trials (RCTs).

Methods: We searched PubMed, Web of Science, Cochrane databases, and ClinicalTrials.gov for RCTs published up to December 2017, on levosimendan for patients undergoing cardiac surgery.

Results: A total of 25 RCTs enrolling 2960 patients met the inclusion criteria; data from 15 placebo-controlled randomized trials were included for meta-analysis. Pooled analysis showed that the all-cause mortality rate was 6.4% (71 of 1106) in the levosimendan group and 8.4% (93 of 1108) in the placebo group (odds ratio [OR], 0.76; 95% confidence interval [CI], 0.55-1.04; P = 0.09). There were no significant differences between the two groups in the rates of myocardial infarction (OR: 0.91; 95% CI, 0.68-1.21; P = 0.52), serious adverse events (OR: 0.84; 95% CI, 0.66-1.07; P = 0.17), hypotension (OR: 1.69; 95% CI, 0.94-3.03; P = 0.08), and low cardiac output syndrome (OR: 0.47; 95% CI, 0.22-1.02; P = 0.05).

Conclusion: Levosimendan did not result in a reduction in mortality in adult cardiac surgery patients. Well designed, adequately powered, multicenter trials are necessary to determine the role of levosimendan in adult cardiac surgery.
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http://dx.doi.org/10.1111/jocs.13716DOI Listing
June 2018

Spatial-temporal signature of resting-state BOLD signals in classic trigeminal neuralgia.

J Pain Res 2017 5;10:2741-2750. Epub 2017 Dec 5.

Department of Neurology, the Second Hospital of Jiaxing City, Jiaxing, Zhejiang.

Resting-state functional magnetic resonance imaging (R-fMRI) signals are spatiotemporally organized. R-fMRI studies in patients with classic trigeminal neuralgia (CTN) have suggested alterations in functional connectivity. However, far less attention has been given to investigations of the local oscillations and their frequency-specific changes in these patients. The objective of this study was to address this issue in patients with CTN. R-fMRI data from 17 patients with CTN and 19 age- and gender-matched healthy controls (HCs) were analyzed using amplitude of low-frequency fluctuation (ALFF). The ALFF was computed across different frequencies (slow-4: 0.027-0.073 Hz; slow-5: 0.01-0.027 Hz; and typical band: 0.01-0.08 Hz) in patients with CTN compared to HCs. In the typical band, patients with CTN showed increases of ALFF in bilateral temporal, occipital, and left middle frontal regions and in the left middle cingulate gyrus, as well as decreases of ALFF in the right inferior temporal region and in regions (medial prefrontal regions) of default mode network. These significant group differences were identified in different sub-bands, with greater brainstem findings in higher frequencies (slow-4) and extensive default mode network and right postparietal results in lower frequencies (slow-5). Furthermore, significant relationships were found between subjective pain ratings and both amplitudes of higher frequency (slow-4) blood oxygen level-dependent (BOLD) signals in pain localization brain regions and lower frequencies (slow-5) in pain signaling/modulating brain regions in the patients, and decreased ALFF within the prefrontal regions was significantly correlated with pain duration in the patients. This result supports our hypothesis that trigeminal pain has a characteristic spatiotemporal distribution of low-frequency BOLD signals. These findings might contribute to a better understanding of the impact of CTN on the brain's intrinsic architecture. Future studies should take the frequencies into account when measuring brain resting BOLD signals of patients with CTN.
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http://dx.doi.org/10.2147/JPR.S143734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5722017PMC
December 2017

LncRNA ZEB2-AS1 promotes bladder cancer cell proliferation and inhibits apoptosis by regulating miR-27b.

Biomed Pharmacother 2017 Dec 24;96:299-304. Epub 2017 Nov 24.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, China.

Background: The aim of this study was to explore the role of lncRNA zinc finger E-box binding homeobox 2 antisense RNA 1 (ZEB2-AS1), as a new tumor-associated lncRNA, in bladder cancer (BC) pathogenesis.

Methods: BC tissues and tumor-adjacent normal bladder tissues were collected for detection of the expression profile of ZEB2-AS1 and miR-27b in BC. The endogenous expression of ZEB2-AS1 and miR-27b was modulated by the recombinant expression vector in vitro. The interaction between ZEB2-AS1 and miR-27b was identified by luciferase report gene assays and RNA immunoprecipitation (RIP) assays. The proliferation and apoptosis of BC cells was determined using CCK-8 assays and flow cytometric analysis.

Results: The expression of ZEB2-AS1 was significantly increased in both BC tissues and BC cells (J82, 5637, T24); while miR-27b was down-regulated in BC tissues. More importantly, ZEB2-AS1 was significantly negative correlated with miR-27b expression in BC tissues (R=0.1688, P<0.05). ZEB2-AS1 silencing inhibited BC cell proliferation and promoted apoptosis. Further studies confirmed that miR-27b was negatively regulated by ZEB2-AS1 in BC cells 5637 and T24, and the effects of ZEB2-AS1 on BC cells was mediated by miR-27b.

Conclusion: Our data provided strong evidence that ZEB2-AS1 promoted tumorigenesis and development of BC through down-regulating tumor-suppressive miR-27b.
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http://dx.doi.org/10.1016/j.biopha.2017.08.060DOI Listing
December 2017

Micro-vesicles derived from human Wharton's Jelly mesenchymal stromal cells mitigate renal ischemia-reperfusion injury in rats after cardiac death renal transplantation.

J Cell Biochem 2018 02 7;119(2):1879-1888. Epub 2017 Sep 7.

Department of Urology, Henan Provincial People's Hospital, Zhengzhou, China.

The purpose of the present study was to investigate the possible therapeutic effects of the human Wharton-Jelly mesenchymal stromal cells derived micro-vesicles (hWJMSCs-MVs) on renal ischemia-reperfusion injury (IRI) after cardiac death (CD) renal transplantation in rats. MVs were injected intravenously in rats immediately after renal transplantation. The animals were sacrificed at 24 h, 48 h, 1 and 2 weeks post-transplantation. ELISA was used to determine the von Willebrand Factor (vWF), tumor necrosis factor (TNF)-α, and interleukin (IL)-10 levels in the serum. Tubular cell proliferation and apoptosis were identified by Ki67 immunostaining and TUNEL assay. Renal fibrosis was assessed by Masson's tri-chrome straining and alpha-smooth muscle actin (α-SMA) staining. The infiltration of inflammatory cells was detected by CD68 staining. The transforming growth factor (TGF)-β, hepatocyte growth factor (HGF), and α-SMA expression in the kidney was measured by Western blot. After renal transplantation, the rats treated with hWJMSCs-MVs improved survival rate and renal function. Moreover, MVs mitigated renal cell apoptosis, enhanced proliferation, and alleviated inflammation at the first 48 h. In the late period, abrogation of renal fibrosis was observed in the MVs group. MVs also could decrease the number of CD68 macrophages in the kidney. Furthermore, MVs decreased the protein expression levels of α-SMA and TGF-β1 and increased the protein expression level of HGF at any point (24 h, 48 h, 1 or 2 weeks). The administration of MVs immediately after renal transplantation could ameliorate IRI in both the acute and chronic stage.
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http://dx.doi.org/10.1002/jcb.26348DOI Listing
February 2018

Engineering Bimetallic Ag-Cu Nanoalloys for Highly Efficient Oxygen Reduction Catalysts: A Guideline for Designing Ag-Based Electrocatalysts with Activity Comparable to Pt/C-20.

Small 2017 05 15;13(19). Epub 2017 Mar 15.

School of Chemistry, University of Birmingham, Birmingham, B15 2TT, UK.

Development of highly active and stable Pt-free oxygen reduction reaction catalysts from earth-abundant elements remains a grand challenge for highly demanded metal-air batteries. Ag-based alloys have many advantages over platinum group catalysts due to their low cost, high stability, and acceptable oxygen reduction reaction (ORR) performance in alkaline solutions. Nevertheless, compared to commercial Pt/C-20%, their catalytic activity still cannot meet the demand of commercialization. In this study, a kind of catalysts screening strategy on Ag Cu nanoalloys is reported, containing the surface modification method, studies of activity enhancement mechanism, and applied research on zinc-air batteries. The results exhibit that the role of selective dealloying (DE) or galvanic displacement (GD) is limited by the "parting limitation", and this "parting limitation" determines the surface topography, position of d-band center, and ORR performance of Ag Cu alloys. The GD-Ag Cu and DE-Ag Cu catalysts alloys present excellent ORR performance that is comparable to Pt/C-20%. The relationship between electronic perturbation and specific activity demonstrates that positive shift of the d-band center (≈0.12 eV, relative to Ag) for GD-Ag Cu is beneficial for ORR, which is contrary to Pt-based alloys (negative shift, ≈0.1 eV). Meanwhile, extensive electrochemical and electronic structure characterization indicates that the high work function of GD-Ag Cu (4.8 eV) is the reason behind their excellent durability for zinc-air batteries.
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http://dx.doi.org/10.1002/smll.201603876DOI Listing
May 2017

Activity Trends of Binary Silver Alloy Nanocatalysts for Oxygen Reduction Reaction in Alkaline Media.

Small 2017 04 2;13(15). Epub 2017 Feb 2.

School of Chemistry, University of Birmingham, Birmingham, B15 2TT, UK.

The electrocatalytic activity of Pt-based alloys exhibits a strong dependence on their electronic structures, but a relationship between electronic structure and oxygen reduction reaction (ORR) activity in Ag-based alloys is still not clear. Here, a vapor deposition based approach is reported for the preparation of Ag M (M = Cu, Co, Fe, and In) and Ag Cu (x = 0, 25, 45, 50, 55, 75, 90, and 100) nanocatalysts and their electronic structures are determined by valence band spectra. The relationship of the d-band center and ORR activity exhibits volcano-shape behaviors, where the maximum catalytic activity is obtained for Ag Cu alloys. The ORR enhancement of Ag Cu alloys originates from the 0.12 eV upshift in d-band center relative to pure Ag, which is different from the downshift in the d-band center in Pt-based alloys. The activity trend for these Ag M alloys is in the order of Ag Cu > Ag Fe > Ag Co . These results provide an insight to understand the activity and stability enhancement of Ag Cu and Ag Cu catalysts by alloying.
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http://dx.doi.org/10.1002/smll.201603387DOI Listing
April 2017

Repurposing the anti-malarial drug dihydroartemisinin suppresses metastasis of non-small-cell lung cancer via inhibiting NF-κB/GLUT1 axis.

Oncotarget 2016 Dec;7(52):87271-87283

Xiamen Diabetes Institution, The First Affiliated Hospital of Xiamen University, Xiamen, People's Republic of China.

Non-small-cell lung cancer (NSCLC) is an aggressive malignancy and long-term survival remains unsatisfactory for patients with metastatic and recurrent disease. Repurposing the anti-malarial drug dihydroartemisinin (DHA) has been proved to possess potent antitumor effect on various cancers. However, the effects of DHA in preventing the invasion of NSCLC cells have not been studied. In the present study, we determined the inhibitory effects of DHA on invasion and migration and the possible mechanisms involved using A549 and H1975 cells. DHA inhibited in vitro migration and invasion of NSCLC cells even in low concentration with little cytotoxicity. Additionally, low concentration DHA also inhibited Warburg effect in NSCLC cells. Mechanically, DHA negatively regulates NF-κB signaling to inhibit the GLUT1 translocation. Blocking the NF-κB signaling largely abolishes the inhibitory effects of DHA on the translocation of GLUT1 to the plasma membrane and the Warburg effect. Furthermore, GLUT1 knockdown significantly decreased the inhibition of invasion, and migration by DHA. Our results suggested that DHA can inhibit metastasis of NSCLC by targeting glucose metabolism via inhibiting NF-κB signaling pathway and DHA may deserve further investigation in NSCLC treatment.
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http://dx.doi.org/10.18632/oncotarget.13536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349987PMC
December 2016

Silver-Copper Nanoalloy Catalyst Layer for Bifunctional Air Electrodes in Alkaline Media.

ACS Appl Mater Interfaces 2015 Aug 4;7(32):17782-91. Epub 2015 Aug 4.

‡Department of Chemistry, University of Birmingham, Birmingham B15 2TT, United Kingdom.

A carbon-free and binder-free catalyst layer composed of a Ag-Cu nanoalloy on Ni foam was used as the air cathode in a zinc-air battery for the first time. The Ag-Cu catalyst was prepared using pulsed laser deposition. The structures of the catalysts were found to consist of crystalline Ag-Cu nanoalloy particles with an average size of 2.58 nm embedded in amorphous Cu films. As observed in the X-ray photoelectron spectra, the Ag 3d core levels shifted to higher binding energies, whereas the Cu 2p core levels shifted to lower binding energies, indicating alloying of the silver and copper. Rotating disk electrode measurements indicated that the oxygen reduction reaction (ORR) proceeded through a four-electron pathway on the Ag50Cu50 and Ag90Cu10 nanoalloy catalysts in alkaline solution. Moreover, the catalytic activity of Ag50Cu50 in the ORR is more efficient than that of Ag90Cu10. By performing charge and discharge cycling measurements, the Ag50Cu50 catalyst layer was confirmed to have a maximum power density of approximately 86.3 mW cm(-2) and an acceptable cell voltage at 0.863 V for current densities up to 100 mA cm(-2) in primary zinc-air batteries. In addition, a round-trip efficiency of approximately 50% at a current density of 20 mA cm(-2) was also obtained in the test.
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http://dx.doi.org/10.1021/acsami.5b04061DOI Listing
August 2015

Oxygen reduction reaction on Cu-doped Ag cluster for fuel-cell cathode.

J Mol Model 2014 Oct 17;20(10):2454. Epub 2014 Sep 17.

State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, People's Republic of China,

The development of fuel cells as clean-energy technologies is largely limited by the prohibitive cost of the noble-metal catalysts needed for catalyzing the oxygen reduction reaction (ORR) in fuel cells. A fundamental understanding of catalyst design principle that links material structures to the catalytic activity can accelerate the search for highly active and abundant bimetallic catalysts to replace platinum. Here, we present a first-principles study of ORR on Ag12Cu cluster in alkaline environment. The adsorptions of O2, OOH, and OH on Cu-doped Ag13 are stronger than on Ag13. The d-band centers of adsorption sites show the Cu-doping makes d-electrons transferred to higher energy state, and improves O2 dissociation. ORR processes on Ag12Cu and Ag13 indicate Cu-doping can strongly promote ORR, and ORR process can be better preformed on Ag12Cu than on Ag13. For four-electron transfer, the effective reversible potential is 0.401 V/RHE on Ag12Cu in alkaline medium.
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http://dx.doi.org/10.1007/s00894-014-2454-7DOI Listing
October 2014

Arabidopsis CPL4 is an essential C-terminal domain phosphatase that suppresses xenobiotic stress responses.

Plant J 2014 Oct 5;80(1):27-39. Epub 2014 Aug 5.

Molecular and Environmental Plant Sciences, Department of Horticultural Sciences, Vegetable and Fruit Development Center, Texas A&M University, College Station, TX, 77843, USA.

Eukaryotic gene expression is both promoted and inhibited by the reversible phosphorylation of the C-terminal domain of RNA polymerase II (pol II CTD). More than 20 Arabidopsis genes encode CTD phosphatase homologs, including four CTD phosphatase-like (CPL) family members. Although in vitro CTD phosphatase activity has been established for some CPLs, none have been shown to be involved in the phosphoregulation of pol II in vivo. Here we report that CPL4 is a CTD phosphatase essential for the viability of Arabidopsis thaliana. Mass spectrometry analysis identified the pol II subunits RPB1, RPB2 and RPB3 in the affinity-purified CPL4 complex. CPL4 dephosphorylates both Ser2- and Ser5-PO(4) of the CTD in vitro, with a preference for Ser2-PO(4). Arabidopsis plants overexpressing CPL4 accumulated hypophosphorylated pol II, whereas RNA interference-mediated silencing of CPL4 promoted hyperphosphorylation of pol II. A D128A mutation in the conserved DXDXT motif of the CPL4 catalytic domain resulted in a dominant negative form of CPL4, the overexpression of which inhibited transgene expression in transient assays. Inhibition was abolished by truncation of the phosphoprotein-binding Breast Cancer 1 C-terminal domain of CPL4, suggesting that both catalytic function and protein-protein interaction are essential for CPL4-mediated regulation of gene expression. We were unable to recover a homozygous cpl4 mutant, probably due to the zygotic lethality of this mutation. The reduction in CPL4 levels in CPL4(RNAi) plants increased transcript levels of a suite of herbicide/xenobiotic-responsive genes and improved herbicide tolerance, thus suggesting an additional role for CPL4 as a negative regulator of the xenobiotic detoxification pathway.
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http://dx.doi.org/10.1111/tpj.12612DOI Listing
October 2014

Memantine combined with environmental enrichment improves spatial memory and alleviates Alzheimer's disease-like pathology in senescence-accelerated prone-8 (SAMP8) mice.

J Biomed Res 2012 Nov 15;26(6):439-47. Epub 2012 Oct 15.

Department of Geriatric Neurology;

Memantine is a N-methyl-D-aspartate (NMDA) receptor antagonist approved for the treatment of moderate to severe Alzheimer's disease (AD). Environmental enrichment (EE) has shown significant beneficial effects on functional improvement in AD. In this study, we sought to determine whether combining these two distinct therapies would yield greater benefit than either drug used alone. We investigated the effect of memantine combined with EE on spatial learning and memory and AD-like pathology in a widely used AD model, the senescence-accelerated prone mice (SAMP8). The SAMP8 mice were randomly assigned to enriched housing (EH) or standard housing (SH), where either memantine (20 mg/kg) or saline was given by gastric lavage once daily continuously for eight weeks. Our results showed that, when provided separately, memantine and EE significantly improved spatial learning and memory by shortening escape latencies and increasing the frequency of entrance into the target quadrant. When combined, memantine and EE showed additive effect on learning and memory as evidenced by significant shorter escape latencies and higher frequency of target entrance than either drug alone. Consistent with the behavior results, pathological studies showed that both memantine and EE significantly reduced hippocampal CA1 neurofibrilliary tangles (NFTs) as well as amyloid beta precursor protein (APP) levels. Combining both therapies synergistically lessened NFTs and APP expression compared to either drug alone in SAMP8 mice, indicating that the combination of memantine with EE could offer a novel and efficient therapeutic strategy for the treatment of AD.
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http://dx.doi.org/10.7555/JBR.26.20120053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597048PMC
November 2012

HnRNPL as a key factor in spermatogenesis: Lesson from functional proteomic studies of azoospermia patients with sertoli cell only syndrome.

J Proteomics 2012 Jun 10;75(10):2879-91. Epub 2012 Jan 10.

Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P R China.

Sertoli cell only syndrome (SCOS) is one of the main causes leading to the abnormal spermatogenesis. However, the mechanisms for abnormal spermatogenesis in SCOS are still unclear. Here, we analyzed the clinical testis samples of SCOS patients by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF/TOF MS) to find the key factors contributing to SCOS. Thirteen differential proteins were identified in clinical testis samples between normal spermatogenesis group and SCOS group. Interestingly, in these differential proteins, Heterogeneous nuclear ribonucleoprotein L(HnRNPL) was suggested as a key regulator involved in apoptosis, death and growth of spermatogenic cells by String and Pubgene bioinformatic programs. Down-regulated HnRNPL in testis samples of SCOS patients was further confirmed by immunohistochemical staining and western blotting. Moreover, in vitro and in vivo experiments demonstrated that knockdown of HnRNPL led to inhibited proliferation, increased apoptosis of spermatogenic cell but decreased apoptosis of sertoli cells. Expression of carcinoembryonic antigen-related cell adhesion molecule 1 in GC-1 cells or expression of inducible nitric oxide synthases in TM4 sertoli cells, was found to be regulated by HnRNPL. Our study first shows HnRNPL as a key factor involved in the spermatogenesis by functional proteomic studies of azoospermia patients with sertoli cell only syndrome. This article is part of a Special Issue entitled: Proteomics: The clinical link.
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http://dx.doi.org/10.1016/j.jprot.2011.12.040DOI Listing
June 2012

One-step casting of Laemmli discontinued sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel.

Anal Biochem 2012 Feb 7;421(1):347-9. Epub 2011 Oct 7.

Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA.

A modified Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protocol is described. The new method saves 30 min for gel casting without loss of the resolution power of Laemmli gel. In this method, both the upper and lower gels can be cast at the same time because the lower gel contains 10% glycerol, which generates higher density in the lower gel than in the upper gel.
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http://dx.doi.org/10.1016/j.ab.2011.10.004DOI Listing
February 2012

The Anabaena sp. strain PCC 7120 asr1734 gene encodes a negative regulator of heterocyst development.

Mol Microbiol 2007 May;64(3):782-94

Department of Biology, Texas A and M University, College Station, TX 77843-3258, USA.

The novel asr1734 gene of Anabaena (Nostoc) sp. strain PCC 7120 inhibited heterocyst development when present in extra copies. Overexpression of asr1734 inhibited heterocyst development in several strains including the wild type and two strains that form multiple contiguous heterocysts (Mch phenotype): a PatS null mutant and a hetR(R223W) mutant. Overexpression of asr1734 also caused increased nblA messenger RNA levels, and increased loss of autofluorescence in vegetative cells throughout filaments after nitrogen or sulphur depletion. Unlike the wild type, an asr1734 knockout mutant formed 5% heterocysts after a nitrogen shift from ammonium to nitrate, and formed 15% heterocysts and a weak Mch phenotype after step-down to medium lacking combined nitrogen. After nitrogen step-down, the asr1734 mutant had elevated levels of ntcA messenger RNA. A green fluorescent protein reporter driven by the asr1734 promoter, P(asr1734)-gfp, was expressed specifically in differentiating proheterocysts and heterocysts after nitrogen step-down. Strains overexpressing asr1734 and containing P(hetR)-gfp or P(patS)-gfp reporters failed to show normal patterned upregulation 24 h after nitrogen step-down even though hetR expression was upregulated at 6 h. Apparent orthologues of asr1734 are found only in two other filamentous nitrogen-fixing cyanobacteria, Anabaena variabilis and Nostoc punctiforme.
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http://dx.doi.org/10.1111/j.1365-2958.2007.05698.xDOI Listing
May 2007

patS minigenes inhibit heterocyst development of Anabaena sp. strain PCC 7120.

J Bacteriol 2004 Oct;186(19):6422-9

Department of Biology, Texas A&M University, College Station, Texas 77843-3258, USA.

The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode PatS C-terminal 4 (GSGR) to 8 (CDERGSGR) oligopeptides. When expressed by P(petE), P(patS), or P(rbcL) promoters, patS5 to patS8 inhibited heterocyst formation but patS4 did not. In contrast to the full-length patS gene, P(hepA)-patS5 failed to restore a wild-type pattern in a patS null mutant, indicating that PatS-5 cannot function in cell-to-cell signaling if it is expressed in proheterocysts. To establish the location of the PatS receptor, PatS-5 was confined within the cytoplasm as a gfp-patS5 fusion. The green fluorescent protein GFP-PatS-5 fusion protein inhibited heterocyst formation. Similarly, full-length PatS with a C-terminal hexahistidine tag inhibited heterocyst formation. These data indicate that the PatS receptor is located in the cytoplasm, which is consistent with recently published data indicating that HetR is a PatS target. We speculated that overexpression of other Anabaena strain PCC 7120 RGSGR-encoding genes might show heterocyst inhibition activity. In addition to patS and hetN, open reading frame (ORF) all3290 and an unannotated ORF, orf77, encode an RGSGR motif. Overexpression of all3290 and orf77 under the control of the petE promoter inhibited heterocyst formation, indicating that the RGSGR motif can inhibit heterocyst development in a variety of contexts.
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http://dx.doi.org/10.1128/JB.186.19.6422-6429.2004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC516582PMC
October 2004

Protection from UV-B damage of mosquito larvicidal toxins from Bacillus thuringiensis subsp. israelensis expressed in Anabaena PCC 7120.

Curr Microbiol 2002 Sep;45(3):217-20

Department of Life Sciences, Ben-Gurion University of the Negav, Be'er-Sheva, Israel.

A transgenic strain of the nitrogen-fixing filamentous cyanobacterium Anabaena PCC 7120 protected expressed delta-endotoxin proteins of Bacillus thuringiensis subsp. israelensis from damage inflicted by UV-B, a sunlight component that penetrates Earth's ozone layer. This organism, which serves as a food source to mosquito larvae and could multiply in their breeding sites, may solve the environment-imposed limitations of B. thuringiensis subsp. israelensis as a mosquito biological control agent.
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http://dx.doi.org/10.1007/s00284-001-0106-5DOI Listing
September 2002
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