Publications by authors named "Xiaoqian Jia"

24 Publications

  • Page 1 of 1

Associations between endocrine-disrupting heavy metals in maternal hair and gestational diabetes mellitus: A nested case-control study in China.

Environ Int 2021 Jul 24;157:106770. Epub 2021 Jul 24.

Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China.

Background: Exposure to environmental endocrine disruptors (EDCs) may lead to abnormal glucose metabolism and, potentially, gestational diabetes mellitus (GDM).

Objective: We investigated the association between five endocrine-disrupting heavy metals (EDHMs), i.e., arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg), and tin (Sn), in maternal hair and the risk of GDM.

Methods: We conducted a nested case-control study including 335 GDM cases and 343 controls without GDM based on a prospective birth cohort established in Beijing, China. Concentrations of EDHMs were analyzed in maternal hair. Log-binomial regression and multiple linear regression were used to estimate the associations between the hair concentrations of single metals and the risk of GDM, while weighted quantile sum (WQS) regression for their mixed effects.

Results: The median concentrations of Hg (0.442 vs. 0.403 μg/g) and Sn (0.171 vs. 0.140 μg/g) in the case group were significantly higher than those in the control group. No differences were found between the two groups for the other three metals. After adjusting for confounders, the prevalence ratio (PR; highest vs. lowest tertile) of GDM risk for Hg was 1.27 (95% confidence interval [CI]: 1.05-1.54), while that for Sn was 1.26 (95% CI: 1.04-1.53). Among women with a body mass index < 24 kg/m, the PR (highest vs. lowest tertile) of GDM for Sn was 1.38 (95% CI: 1.09-1.75). The effect of exposure to the five EDHMs on the risk of GDM was estimated by WQS regression: Sn and Hg made the largest contributions to the WQS index (40.9% and 40.3%, respectively).

Conclusion: High maternal levels of EDHMs, particularly Sn and Hg, may promote the development of GDM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2021.106770DOI Listing
July 2021

NADPH-Oxidase 2 Promotes Autophagy in Spinal Neurons During the Development of Morphine Tolerance.

Neurochem Res 2021 Aug 18;46(8):2089-2096. Epub 2021 May 18.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Repeated morphine administration results in analgesic tolerance. However, the underlying mechanism of morphine analgesic tolerance remains unclear. NADPH-oxidase 2 (NOX2) is the first discovered NADPH oxidase, which mainly functions to produce reactive oxygen species. Its specific role in morphine tolerance has not been fully investigated. In this work, we found that chronic morphine administration significantly increased the expression of NOX2 in spinal cord. Pretreatment of NOX2 inhibitor blocked the upregulation of NOX2 and autophagy markers, including LC3B and P62, and consequently the development of morphine tolerance. NOX2 and LC3B were both colocalized with NeuN in spinal dorsal horn in morphine-tolerant rats. Our results suggest that the increased autophagy activity in spinal neurons promoted by NOX2 activation contributes to the development of morphine tolerance. NOX2 may be considered as a new therapeutic target for morphine tolerance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11064-021-03347-5DOI Listing
August 2021

Individualized protocol for radiation and contrast medium dose reduction in one-stop assessment for kidney transplantation patients.

Eur J Radiol 2021 Jul 7;140:109757. Epub 2021 May 7.

Department of Radiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address:

Objective: To compare image quality, radiation, and contrast medium (CM) doses between individualized and conventional scan protocols in combined coronary CT angiography (CCTA) and iliac artery CTA for kidney transplantation patients.

Methods: 148 patients needing assessment for coronary and iliac arteries before kidney transplantation were prospectively enrolled and randomly divided into the conventional and individualized groups. All patients underwent one-stop combined scans on a 256-row CT scanner with automatic tube current modulation, 50 % pre-ASIR-V to control radiation dose. CCTA was performed first using one heartbeat axial scan mode with bolus tracking technique and iliac CTA was performed 3 s after CCTA using a spiral scan. The conventional group (n = 72) used the standard protocol: 100 kVp, 60 mL of 350 mgI/mL CM at 4.5 mL/s flow rate. The individualized group (n = 76) used a body-mass-index (BMI)-dependent protocol: kVp: 80 (BMI < 24) and 100 (BMI ≥ 24) and CM: 19 mgI/kg (BMI < 18); 21 mgI/kg (18 ≤ BMI < 24); and 22 mgI/kg (BMI ≥ 24). Image quality radiation and CM doses of the two groups were compared.

Results: There was no significant difference in patient demographic data. Compared with the conventional group, the individualized group reduced contrast flow rate (in mL/s) by 14.4 % (3.85 ± 0.72 vs. 4.5), contrast dose (in mL) by 35.8 % (38.53 ± 7.18 vs. 60) and radiation dose (in mSv) by 34.3 % (4.30 ± 1.73 vs. 6.54 ± 1.45). The individualized group had significantly higher subjective image quality score (P < 0.05), lower noise (17.30 ± 4.97 HU vs. 19.13 ± 4.73 HU, P = 0.02) and higher signal-to-noise ratio (22.09 ± 7.41 vs. 19.55 ± 6.18, P = 0.03) for the three main vessels in CCTA compared with the conventional group. There were no differences in both subjective scores and objective measurements in iliac artery CTA between the two groups.

Conclusion: The individualized scanning protocol in the one-stop assessment of coronary and iliac arteries before kidney transplantation significantly reduces both radiation and CM doses while maintaining image quality in iliac artery CTA and providing better coronary artery images in CCTA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejrad.2021.109757DOI Listing
July 2021

Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats.

J Neurochem 2021 Jul 2;158(2):373-390. Epub 2021 Jun 2.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The adverse side effects of opioids, especially antinociceptive tolerance, limit their clinical application. A recent study reported that platelet-derived growth factor receptor β (PDGFRβ) blockage selectively inhibited morphine tolerance. Autophagy has been reported to contribute to the cellular and behavioral responses to morphine. However, little is known about the relationship between PDGFRβ and autophagy in the mechanisms of morphine tolerance. In this study, rats were intrathecally administered with morphine twice daily for 7 days to induce antinociceptive tolerance, which was evaluated using a tail-flick latency test. By administration autophagy inhibitor 3-Methyladenine, PDGFRβ inhibitor imatinib, p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580 hydrochloride and minocycline hydrochloride, western blot, immunofluorescence, and transmission electron microscopy techniques were used to elucidate the roles of PDGFRβ, autophagy, and related signaling pathways in morphine tolerance. This study demonstrated for the first time that spinal PDGFRβ in microglia promotes autophagy in gamma-aminobutyric acid (GABA) interneurons through activating p38 MAPK pathway during the development of morphine tolerance, which suggest a potential strategy for preventing the development of morphine tolerance clinically, thereby improving the use of opioids in pain management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jnc.15383DOI Listing
July 2021

Evaluation of the lignite biotransformation capacity of sp. NF01 cultured on different growth substrates.

Can J Microbiol 2021 Aug 22;67(8):613-621. Epub 2021 Mar 22.

School of Energy, Xi'an University of Science and Technology, Xi'an, Shaanxi 710054, P.R. China.

The screening and studying the lignite solubilization/degradation capacities of indigenous microorganisms are key to exploring the in-situ biotransformation of lignite. Herein, a fungus was isolated from in-situ lignite samples and identified as sp. NF01. This isolate was then cultured on four different carbon sources to evaluate its lignite-transformation capacity. When cultured on a solid agar medium containing sodium gluconate or sodium glutamate, sp. NF01 completely liquefied 0.5 g of lignite within 6 days, and when cultured in a liquid medium containing sodium gluconate, the weight of lignite decreased by 28.4% within 7 days. Elemental analysis showed that the rate of lignite biodegradation was inversely proportional to the C:O ratio of the residual lignite samples. Additionally, a 5.9% biodesulfurization rate was achieved when sp. NF01 was cultured in the presence of sodium gluconate. Finally, Fourier-transform infrared analysis of the residual lignite samples revealed relatively weak signal intensities of the signature peaks representing the following: aromatic ring side chains; ether, ester, and alcohol bonds; aromatic ring carbon-carbon double bonds; and aliphatic methyl and methylene. The results show that sp. NF01 degrades lignite in a carbon-dependent manner and could be thus used for the bioconversion of subsurface coalbeds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1139/cjm-2020-0157DOI Listing
August 2021

Lower Limb Joint Nursing and Rehabilitation System Based on Intelligent Medical Treatment.

J Healthc Eng 2021 9;2021:6646977. Epub 2021 Feb 9.

Department of Joint Surgery, The Fourth People's Hospital of Jinan, Jinan 250031, Shandong, China.

With the aggravation of the problem of aging population, all kinds of lower limb paralysis caused by various diseases occur frequently. People's demand for lower limb nursing and rehabilitation treatment is growing. In this paper, combined with intelligent medical technology and lower limb kinematics model, this paper proposes to build a lower limb joint nursing and rehabilitation system based on intelligent medical treatment. It is expected that, through the following limb joint rehabilitation robot as the main rehabilitation means, a smart nursing rehabilitation system which can quickly respond to users and realize remote rehabilitation nursing can be designed. First of all, it is clear that the main body of the lower limb joint rehabilitation system consists of the robot body and the state display system. Then, the sensor, amplifier, and data acquisition card are set in the data acquisition system, and the plantar balance force is detected using a FlexiForce film pressure sensor. The final control system mainly includes the main control module program and the lower limb action recognition program. The motor control software adopts PID regulation method, and the lower limb action recognition adopts SVM one-to-one classification method. After the construction of lower limb joint nursing and rehabilitation system, the accuracy rate of action recognition and classification was tested. In the third experiment, the accuracy of all the movements was 100%. Then, the joint displacement and angle changes of the experimenter assisted by the system were analyzed. The experimenter's knee joint and hip joint show a normal walking state, and the joint angle changes tend to be normal. Ten out of 55 rehabilitation system users were randomly selected for interview survey. The total scores of operation convenience, wearing comfort, intensity suitability, and movement science of the system were 90, 83, 84, and 91, respectively. This shows that the rehabilitation action designed by the system is scientific and easy to operate and can be put into use in rehabilitation training after improving the wearing comfort.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6646977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886520PMC
February 2021

Low-dose CT urography using deep learning image reconstruction: a prospective study for comparison with conventional CT urography.

Br J Radiol 2021 Apr 24;94(1120):20201291. Epub 2021 Feb 24.

Department of Radiology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi province, PR China.

Objectives: To compare the image quality of low-dose CT urography (LD-CTU) using deep learning image reconstruction (DLIR) with conventional CTU (C-CTU) using adaptive statistical iterative reconstruction (ASIR-V).

Methods: This was a prospective, single-institutional study using the excretory phase CTU images for analysis. Patients were assigned to the LD-DLIR group (100kV and automatic mA modulation for noise index (NI) of 23) and C-ASIR-V group (100kV and NI of 10) according to the scan protocols in the excretory phase. Two radiologists independently assessed the overall image quality, artifacts, noise and sharpness of urinary tracts. Additionally, the mean CT attenuation, signal-to-noise ratio (SNR) and contrast-to-noise (CNR) in the urinary tracts were evaluated.

Results: 26 patients each were included in the LD-DLIR group (10 males and 16 females; mean age: 57.23 years, range: 33-76 years) and C-ASIR-V group (14 males and 12 females; mean age: 60 years, range: 33-77 years). LD-DLIR group used a significantly lower effective radiation dose compared with the C-ASIR-V group (2.01 ± 0.44 mSv 6.9 ± 1.46 mSv, < 0.001). LD-DLIR group showed good overall image quality with average score >4 and was similar to that of the C-ASIR-V group. Both groups had adequate and similar attenuation value, SNR and CNR in most segments of urinary tracts.

Conclusion: It is feasibility to provide comparable image quality while reducing 71% radiation dose in low-dose CTU with a deep learning image reconstruction algorithm compared to the conventional CTU with ASIR-V.

Advances In Knowledge: (1) CT urography with deep learning reconstruction algorithm can reduce the radiation dose by 71% while still maintaining image quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1259/bjr.20201291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010546PMC
April 2021

Contribution of Temperature Increase to Restrain the Transmission of COVID-19.

Innovation (N Y) 2021 Feb 16;2(1):100071. Epub 2020 Dec 16.

Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, P. R. China.

The COVID-19 outbreak has already become a global pandemic and containing this rapid worldwide transmission is of great challenge. The impacts of temperature and humidity on the COVID-19 transmission rate are still under discussion. Here, we elucidated these relationships by utilizing two unique scenarios, repeated measurement and natural experiment, using the COVID-19 cases reported from January 23 - February 21, 2020, in China. The modeling results revealed that higher temperature was most strongly associated with decreased COVID-19 transmission at a lag time of 8 days. Relative humidity (RH) appeared to have only a slight effect. These findings were verified by assessing SARS-CoV-2 infectivity under the relevant conditions of temperature (4°C-37°C) and RH (> 40%). We concluded that temperature increase made an important, but not determined, contribution to restrain the COVID-19 outbreak in China. It suggests that the emphasis of other effective controlling polices should be strictly implemented to restrain COVID-19 transmission in cold seasons.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.xinn.2020.100071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7834433PMC
February 2021

Association between tea drinking and plasma folate concentration among women aged 18-30 years in China.

Public Health Nutr 2021 Oct 15;24(15):4929-4936. Epub 2020 Dec 15.

Institute of Reproductive and Child Health/National Health Commission Key Laboratory of Reproductive Health, Peking University Health Science Center, Beijing100191, China.

Objective: Association was found between tea and neural tube defects. However, few studies investigated the relationship between tea consumption and blood folate levels. We aimed to investigate the association between tea consumption and plasma folate concentrations among women aged 18-30 years in different ethnicities of China.

Design: Data were obtained from a national cross-sectional study conducted from 2005 to 2006 of women aged 18-30 years in China. Socio-demographic characteristics and lifestyle were obtained from a questionnaire. Dietary folate intake was determined by 24-h dietary recall. Plasma folate concentrations were measured by a microbiological assay. Multiple linear regression model was used to calculate partial regression coefficients after adjusting for confounding factors.

Setting: Nine provinces or autonomous regions in China.

Participants: A total of 2932 women aged 18-30 years in China.

Results: After stratifying by ethnicity and tea type, tea consumption was significantly positively associated with plasma folate levels in Han women who drank unfermented tea weekly (β = 0·067, and P = 0·037) or daily (β = 0·119, and P = 0·031) and in Uighur women who drank fermented tea weekly (β = 0·325, and P = 0·028). For women who drank unfermented tea in Han ethnicity, weekly and daily tea drinkers had 6·77 % (95 % CI: 6·36 %, 7·21 %) and 7·13 % (95 % CI: 6·40 %, 7·96 %) increase in plasma folate concentration compared with no tea drinkers.

Conclusions: There is a suggestion of possible positive association between unfermented tea drinking in Han ethnicity and plasma folate concentrations, for Chinese women aged 18-30 years. The relationship between tea drinking in other ethnic groups and plasma folate still needs to be further explored.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1017/S1368980020004851DOI Listing
October 2021

Prevalence of mental health problems during the COVID-19 pandemic: A systematic review and meta-analysis.

J Affect Disord 2021 02 3;281:91-98. Epub 2020 Dec 3.

Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, China; National Health Commission Key Laboratory of Reproductive Health, Beijing, China. Electronic address:

Background: The global COVID-19 pandemic has generated major mental and psychological health problems worldwide. We conducted a meta-analysis to assess the prevalence of depression, anxiety, distress, and insomnia during the COVID-19 pandemic.

Methods: We searched online biomedical databases (PubMed, Embase, Web of Science, Ovid, CNKI, and Wanfang Data) and preprint databases (SSRN, bioRxiv, and MedRxiv) for observational studies from January 1, 2020 to March 16, 2020 investigating the prevalence of mental health problems during the COVID-19 pandemic.

Results: We retrieved 821 citations from the biomedical databases and 53 citations from the preprint databases: 66 studies with 221,970 participants were included in our meta-analysis. The overall pooled prevalence of depression, anxiety, distress, and insomnia was 31.4%, 31.9%, 41.1% and 37.9%, respectively. Noninfectious chronic disease patients, quarantined persons, and COVID-19 patients had a higher risk of depression (Q=26.73, p<0.01) and anxiety (Q=21.86, p<0.01) than other populations. The general population and non-medical staff had a lower risk of distress than other populations (Q=461.21, p< 0.01). Physicians, nurses, and non-medical staff showed a higher prevalence of insomnia (Q=196.64, p<0.01) than other populations.

Limitations: All included studies were from the early phase of the global pandemic. Additional meta-analyses are needed to obtain more data in all phases of the pandemic.

Conclusions: The COVID-19 pandemic increases the mental health problems of the global population, particularly health care workers, noninfectious chronic disease patients, COVID-19 patients, and quarantined persons. Interventions for mental health are urgently needed for preventing mental health problems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jad.2020.11.117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710473PMC
February 2021

Modeling the Prevalence of Asymptomatic COVID-19 Infections in the Chinese Mainland.

Innovation (N Y) 2020 Aug 4;1(2):100026. Epub 2020 Aug 4.

Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, P. R. China.

Recently, considerable efforts have been focused on intensifying the screening process for asymptomatic COVID-19 cases in the Chinese Mainland, especially for up to 10 million citizens living in Wuhan City by nucleic acid testing. However, a high percentage of domestic asymptomatic cases did not develop into symptomatic ones, which is abnormal and has drawn considerable public attention. Here, we aimed to investigate the prevalence of COVID-19 infections in the Chinese Mainland from a statistical perspective, as it is of referential significance for other regions. By conservatively assuming a development time lag from pre-symptomatic (i.e., referring to the infected cases that were screened before the COVID-19 symptom onset) to symptomatic as an incubation time of 5.2 days, our results indicated that 92.5% of those tested in Wuhan City, China, and 95.1% of those tested in the Chinese Mainland should have COVID-19 syndrome onset, which was extremely higher than their corresponding practical percentages of 0.8% and 3.3%, respectively. We propose that a certain false positive rate may exist if large-scale nucleic acid screening tests for asymptomatic cases are conducted in common communities with a low incidence rate. Despite adopting relatively high-sensitivity, high-specificity detection kits, we estimated a very low prevalence of COVID-19 infections, ranging from 10 to 10 in both Wuhan City and the Chinese Mainland. Thus, the prevalence rate of asymptomatic infections in China had been at a very low level. Furthermore, given the lower prevalence of the infection, close examination of the data for false positive results is necessary to minimize social and economic impacts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.xinn.2020.100026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399709PMC
August 2020

Novel Core-Shell (ε-MnO/CeO)@CeO Composite Catalyst with a Synergistic Effect for Efficient Formaldehyde Oxidation.

ACS Appl Mater Interfaces 2020 Sep 25;12(36):40285-40295. Epub 2020 Aug 25.

College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot 010051, China.

A novel core-shell (ε-MnO/CeO)@CeO composite catalyst with a synergistic effect was prepared by hydrothermal reaction and thermal decomposition and its application to high-efficiency oxidation removal of formaldehyde (HCHO) was systemically investigated. The (MnCO/CeO)@CeO precursor was prepared first by the one-pot hydrothermal reaction of Mn and Ce solutions with a CO-storage material (COSM) without any external templates or surfactants required. The thermal decomposition of the precursor afforded the core-shell (ε-MnO/CeO)@CeO composite catalyst with excellent catalytic performance. HCHO in the feed gas (180 ppm HCHO, 21% O, N balanced) at a gas hourly space velocity of 100 L/(g h) is 100% converted over the catalyst at 80 °C. The conversion rate remains above 95% in 72 h and above 73.8% in 140 h, suggesting the strong stability of the catalyst at high gas flow rates and relatively low temperatures. The synergistic mechanism of the catalyst was explored by X-ray diffraction, Raman, Brunauer-Emmett-Teller, transmission electron microscopy, and X-ray photoelectron spectroscopy. The number of defects in the catalyst and the strength of the Mn-O bond in ε-MnO can be tuned by adjusting the synthesis conditions. More oxygen vacancies on the surface of CeO can make the synergistic effect of the catalyst stronger, which significantly improves the lattice oxygen (O) activity on the surface of ε-MnO. Our work has provided new insights into the preparation of the desired composite catalysts with excellent performances.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c09263DOI Listing
September 2020

The Interaction Between Spinal PDGFRβ and μ Opioid Receptor in the Activation of Microglia in Morphine-Tolerant Rats.

J Pain Res 2020 17;13:1803-1810. Epub 2020 Jul 17.

Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Purpose: Opioid tolerance remains a challenging problem, which limits prolonged drug usage in clinics. Previous studies have shown a fundamental role of platelet-derived growth factor receptor β submit (PDGFRβ) in morphine tolerance. The aim of this study was to investigate the mechanisms of spinal PDGFRβ activation in morphine tolerance.

Methods: Rats were treated with morphine for 7 days and the effect of drug was evaluated by tail-flick latency test. By using Western blot and real-time PCR, the interaction between μ opioid receptor (MOR) and PDGFRβ in microglia activation, as well as related signaling pathways during morphine tolerance were investigated.

Results: Chronic PDGFRβ agonist could induce microglia activation in spinal cord and decrease the analgesic effect of morphine. PDGFRβ inhibitor suppressed microglia activation during the development of morphine tolerance. Furthermore, antagonizing MOR could effectively inhibit the phosphorylations of PDGFRβ and JNK. Blocking PDGFRβ had no influence on JNK signaling, while JNK inhibitor could decrease the phosphorylation of PDGFRβ.

Conclusion: These results provide direct evidence that repeatedly activating MOR by morphine could induce the transactivation of PDGFRβ via JNK MAPK in spinal cord, which leads to microglia activation during the development of morphine tolerance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/JPR.S255221DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7381827PMC
July 2020

Spinal heat shock protein 27 participates in PDGFRβ-mediated morphine tolerance through PI3K/Akt and p38 MAPK signalling pathways.

Br J Pharmacol 2020 11 30;177(22):5046-5062. Epub 2020 Sep 30.

Department of Anesthesiology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Background And Purpose: The development of antinociceptive morphine tolerance is a clinically intractable problem. Earlier work has demonstrated the pivotal roles of PDGF and its receptor PDGFRβ in morphine tolerance. Here, we have investigated the role of spinal heat shock protein 27 (HSP27) in morphine tolerance and its relationship with PDGFRβ activation.

Experimental Approach: Rats were treated with morphine for 9 days, and its anti-nociceptive effect against thermal pain was evaluated by a tail-flick latency test. Western blot, real-time PCR, immunofluorescent staining, and various antagonists, agonists, and siRNA lentiviral vectors elucidated the roles of HSP27, PDGFRβ, and related signalling pathways in morphine tolerance.

Key Results: Chronic morphine administration increased expression and phosphorylation of HSP27 in the spinal cord. Down-regulating HSP27 attenuated the development of morphine tolerance. PDGFRβ antagonism inhibited HSP27 activation and attenuated and reversed morphine tolerance. PDGFRβ induction increased HSP27 expression and activation and partly decreased morphine analgesia. PDGFRβ inhibition reduced Akt and p38 MAPK activity in morphine tolerance. PI3K and p38 inhibitors reversed morphine tolerance and suppressed morphine-induced HSP27 phosphorylation.

Conclusion And Implications: This study demonstrated for the first time that spinal HSP27 participates in PDGFRβ-mediated morphine tolerance via the PI3K/Akt and p38 MAPK signalling pathways. These findings suggest a potential clinical strategy for prolonging the antinociceptive effects of opioids during long-term pain control.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/bph.15169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589020PMC
November 2020

Improving diagnostic accuracy for arteries of lower extremities with dual-energy spectral CT imaging.

Eur J Radiol 2020 Jul 12;128:109061. Epub 2020 May 12.

Department of Radiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, PR China. Electronic address:

Purpose: Investigate the clinical value of improving diagnostic accuracy for arteries of lower extremities with low energy images in dual-energy spectral CT (DEsCT) imaging.

Method: 110 (mean age, 67 ± 10 years) and 72 (mean age, 65 ± 13 years) patients underwent CT angiography (CTA) in the lower extremities using dual-energy and conventional (100kVp) imaging mode, retrospectively. The 50 keV monochromatic images were reconstructed in the DEsCT group for analysis. The quantitative and qualitative image quality of the two groups were compared using various statistical analysis methods and the diagnostic accuracy for the degree of vessel stenosis was compared using DSA as the gold standard. Consistency test was used for intra-group evaluation. A P < 0.05 was considered statistically significant.

Results: The use of 50 keV images in DEsCT significantly increased the enhancement in arteries of LKS (544.91 ± 106.37HU vs. 339.65 ± 83.74HU, P < 0.001) and provided higher SNR (19.92±9.39 vs. 17.39±4.99, P = 0.04) and CNR (45.60±16.61 vs. 38.70±18.17, P < 0.01) compared with conventional 100kVp images. Mann-Whitney test showed that the subjective image quality of the arteries of lower knee segment (LKS) in the DEsCT group was higher than in the conventional group (P = 0.01). The diagnostic efficiency of DEsCT group was better than that of conventional group, mainly in arteries of LKS (95.91 % vs. 87.85 %, for 50 % stenosis, P<0.001; 94.32 % vs. 89.58 % for occlusion, P = 0.02).

Conclusions: The use of 50 keV DEsCT images enhances the contrast in the lower extremity arteries and improves the diagnostic accuracy for the arteries of LKS, compared with the conventional CTA protocols.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejrad.2020.109061DOI Listing
July 2020

Environmental complex exposure and the risk of influenza-like illness among housewives: A case study in Shanxi Province, China.

Ecotoxicol Environ Saf 2020 May 9;194:110405. Epub 2020 Mar 9.

Reproductive Medical Center, Peking University People's Hospital, Beijing, 100044, PR China. Electronic address:

The association between environmental pollution and risk of influenza-like illness (ILI) among general population has been reported. However, the relationships between the individual pollutants and ILI risk are still under discussion. Our study aimed to explore the associations of the typical environmental polycyclic aromatic hydrocarbons (PAHs) and metal(loid)s with ILI risk among women population. We carried out a cross-sectional study and included a total of 396 housewives in Shanxi Province, China. The information on their general characteristics and ILI frequency was collected by questionnaire. We collected their hair samples and analyzed the concentrations of PAHs and various metal(loid)s. The results indicated that only acenaphthylene concentration of the nine detected PAH congeners in the hair was significantly associated with ILI risk with adjusted odds ratio (AOR) and 95% confidence interval (95% CI) of 0.58 (0.38 - 0.91). Among the concerned 4 toxic metal(loid)s and 15 rare earth elements, only the hair concentration of arsenic had a positive dose-response relationship with ILI risk. In addition, we found that there were negative dose-response associations of the three essential trace elements (i.e. chromium, cobalt, and nickel), and four essential alkaline earth elements (i.e. magnesium, calcium, strontium, barium) with ILI risk. It was concluded that the environmental exposure to certain compounds of housewives may contribute to their ILI development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.110405DOI Listing
May 2020

External interference from ambient air pollution on using hair metal(loid)s for biomarker-based exposure assessment.

Environ Int 2020 04 24;137:105584. Epub 2020 Feb 24.

Institute of Reproductive and Child Health, Peking University/Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China. Electronic address:

Hair metal(loid)s are often measured as biomarkers to evaluate population internal exposure, however, hair samples could be easily contaminated by ambient particulate matter (PM) pollution. Here, we evaluated the potential external interference from ambient PM pollution on using hair metal(loid)s for population biomarker-based exposure assessment. The raw hair samples were strictly washed and placed under various indoor and outdoor scenarios for ~6 months at sites with high PM pollution. The contaminated hair was then washed using the same method. A total of 33 hair elements were quantified by inductively coupled plasma-mass spectrometry. The surface residual PM on hair after washing was observed by scanning electron microscopy. In addition, we chose a practical exposure scenario including 77 housewives in Shanxi Province, China for validation. The results for the hair exposure experiment revealed that external contamination of some elements that had relatively high concentrations in hair was generally mild in both indoor and outdoor exposure scenarios (i.e., Zn, Mg, Se, Fe, Sr, Ti, Mn, Sn, Ge, U, Co, Mo, and As). A relatively higher external contamination of other elements (e.g., Al, Cr, Pb, Cd, Li, and most rare earth elements (REEs)) was observed, especially for those elements with relatively low hair concentrations (e.g., Cd, and REEs) in the outdoor environment. This finding was due mainly to some small ambient PM not being fully removed by the current washing strategy when the hair sample was heavily contaminated. However, results from practical exposure scenario of the housewives showed that there were overall no significant differences of hair metal(loid)s between the housewives using coal and clean energy for cooking. We concluded that the external interference on hair internal metal(loid) analysis could be negligible when hair was efficiently washed, especially for population with relatively longer indoor activities. It is therefore promising to use hair analysis for their population exposure assessment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envint.2020.105584DOI Listing
April 2020

Simultaneous analysis of typical halogenated endocrine disrupting chemicals and metal(loid)s in human hair.

Sci Total Environ 2020 May 13;718:137300. Epub 2020 Feb 13.

Institute of Reproductive and Child Health, Peking University, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China.

Hair analysis has been an important approach in evaluating population exposure to various environmental factors. To meet the requirements of human environmental epidemiology studies, we aimed to develop an efficient method for simultaneous analysis of various metal(loid)s and some typical environmental halogenated endocrine disrupting chemicals (hEDCs) (i.e., polychlorinated biphenyls, polybrominated diphenyl ethers, and organochlorine pesticides, as well as some of their hydroxyl substituted metabolites) in a single hair sample. The hair was washed successively with surfactant solutions, methanol solvent, and deionized water to remove impurities attached to the hair surface. Efficiency was comprehensively compared among various washing strategies. The hair sample was further pulverized into fine powder with a median diameter (25th-75th percentile) of 8.6 (5.9-13.5) μm. The hair organic components were extracted by acetonitrile solvent and compared with the microwave-assisted extraction method. The hEDCs in the supernatant acetonitrile phase were quantified by gas chromatography-mass spectrometry, and the metal(loid)s in the precipitate hair were further analyzed by inductively coupled plasma mass spectrometry. Our developed method was further applied to analyze the hair samples of 165 pregnant women. The results showed that particles attached to the surface of the hair could not be washed off completely. However, we proposed a protocol framework to wash hair with relatively high efficience, which includes warm water incubation, and use of surfactant and organic solvent. The recoveries of the concerned hEDCs and metal(loid)s were overall in the range of 80% to 120%. For the women population, the method can efficiently recognize the typical exposure characteristics of the concerned hEDCs and metal(loid)s. Our study significantly ameliorated the deficiencies of the traditional hair washing strategy and developed an efficient method for simultaneous analysis of various metal(loid)s and hEDCs in a single hair sample. This method will provide important support for population complex exposure analysis and facilitate environmental exposome studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.137300DOI Listing
May 2020

Organochlorine pesticides exposure may disturb homocysteine metabolism in pregnant women.

Sci Total Environ 2020 Mar 21;708:135146. Epub 2019 Nov 21.

Institute of Reproductive and Child Health/Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Peking University, Beijing 100191, China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, China. Electronic address:

Maternal exposure to organochlorine pesticides (OCPs) has an adverse impact on maternal and fetal health, and excessive homocysteine is related to a variety of adverse pregnancy outcomes. Biomimetic studies suggest that OCPs interfere with folate-dependent pathways, but little evidence is available from studies with human subjects. This study explored whether exposure to OCPs interferes with the metabolism of homocysteine, which is folate dependent. A total of 313 pregnant women at 12-20 weeks gestation were recruited in Shanxi province, China, from 2014 to 2015. Plasma concentrations of 20 OCPs, including dichlorodiphenyltrichloroethane and metabolites (DDTs), hexachlorobenzene (HCB), and hexachlorocyclohexanes (HCHs), were analyzed by gas chromatography-mass spectrometry. Blood folate concentrations were analyzed by microbiological assay, and plasma homocysteine concentrations were determined by enzyme-linked immunosorbent assay. Information on demographics, lifestyle behaviors, and folic acid supplementation was collected by in-person interview. Of the women, 99% reported having taken folic acid supplements. Results of a logistic regression analysis showed that higher plasma levels of OCPs were associated with increased odds of higher plasma homocysteine after adjustment for potential confounding factors. Positive correlations were observed between plasma OCPs and plasma homocysteine concentrations: HCB (r = 0.176, p = 0.002), β-HCH (r = 0.172, p = 0.002), ρ,ρ'-DDE (r = 0.132, p = 0.020), ρ,ρ'-DDD (r = 0.161, p = 0.004), and ο,ρ'-DDT (r = 0.144, p = 0.011). Plasma concentrations of OCPs were negatively correlated with red blood cell (RBC) folate in the low-RBC-folate subgroup, but the correlations were not statistically significant. A positive correlation was observed between OCPs and homocysteine in the low-RBC-folate subgroup. These findings suggest that OCPs may disturb the folate-dependent homocysteine metabolism pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.135146DOI Listing
March 2020

Potential effect of germanium exposure on the risk of influenza-like illness in housewives in Shanxi Province, China.

Sci Total Environ 2019 Sep 9;682:208-212. Epub 2019 May 9.

Institute of Reproductive and Child Health, Peking University/Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, PR China.

Few studies have examined the relationship between exposure to germanium (Ge) and the risk of influenza-like illness (ILI). Therefore, we investigated the association of Ge exposure and its interaction with single nucleotide polymorphisms (SNPs) related to Phase II metabolism on ILI risk among housewives in Shanxi Province, northern China. This cross-sectional study enrolled 373 housewives. Information on the housewives' characteristics and the frequency of ILI was collected by questionnaire. We analyzed the Ge concentrations in hair samples taken from near the scalp at the back of the head. Blood samples were used to identify SNPs related to Phase II metabolism. The results suggested that the hair Ge concentration was associated with ILI risk with an adjusted odds ratio and 95% confidence interval of 2.59 (1.61-4.19). A significant dose-response relationship was observed without or with adjusting for confounders. We did not observe any interaction effect between the hair Ge concentration and the SNPs on ILI risk. We found that high dietary consumption of meat and fried foods was positively correlated with the hair Ge concentration. Therefore, chronic Ge exposure may be a risk factor for an increased frequency of ILI in housewives.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.05.103DOI Listing
September 2019

An efficient method to simultaneously analyze multi-class organic pollutants in human serum.

Environ Pollut 2019 Aug 3;251:400-406. Epub 2019 May 3.

Institute of Reproductive and Child Health, Peking University/ Key Laboratory of Reproductive Health, National Health Commission of the People's Republic of China, Beijing, 100191, PR China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, 100191, PR China.

The degree of population exposure to various organic pollutants (OPs), including polycyclic aromatic hydrocarbons, organochlorinated pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers, can be determined by measuring their concentrations in human serum. However, performing large-scale measurements with such a variety of compounds in serum is challenging in terms of efficiency and cost. We describe herein the development of a high-efficiency extraction and sample cleanup protocol for simultaneous and quantitative analyses of OPs using gas chromatography-mass spectrometry. OPs, together with crude lipid impurities, were extracted from human serum with a mixture of n-hexane and methyl tert-butyl ether. A disperse sorbent composed of primary secondary amine and C18 (PSA/C18) was used to roughly remove co-extracted impurities. A combined column of neutral silica gel and neutral alumina oxide (AlO/SiG) was then used for deep cleanup. For the removal of impurities, the overall performance of our protocol for the analysis of OPs in serum was comparable to that of traditional gel permeation chromatography (GPC) and dramatically better than that of PSA/C18, which is a frequently used QuEChERS (quick, easy, cheap, effective, rugged, safe) based method. While both the proposed protocol and GPC yielded recoveries of 80%-110% for four classes of OPs, our protocol consumed about 10 times less solvent, resulting in lower experimental expenses and a lower risk of contamination from residual OPs in the solvent and other supplies. In contrast to GPC, our protocol also permits efficient batch processing of serum samples, allowing for large sample sizes such as those encountered in epidemiological studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2019.05.008DOI Listing
August 2019

Improving the Degree and Uniformity of Enhancement in Coronary CT Angiography with a New Bolus Tracking Method Enabled By Free Breathing.

Acad Radiol 2019 12 4;26(12):1591-1596. Epub 2019 Mar 4.

Department of Radiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, PR China. Electronic address:

Rationale And Objectives: To demonstrate the improved enhancement degree and uniformity in coronary CT angiography (CCTA) on a 16 cm wide-coverage CT with a new bolus tracking method enabled by free-breathing, in comparison with the conventional breath-holding method.

Materials And Methods: A total of 200 patients with suspected coronary heart disease were randomly divided into two groups for CCTA: Group A (n = 100, free-breathing) started CCTA with 2.2 seconds delay after the attenuation in aorta reached 250 HU; Group B (n = 100, breath-holding), used the standard protocol of 80 HU threshold and 8.4 seconds delay. Both groups used the contrast dose rate of 25 mgI/kg/s. CT value and standard deviation in aortic sinus (AS), right coronary artery, left anterior descending, left circumflex, and pericardial fat were measured. Contrast-to-noise ratio for vessels was calculated. Two experienced Radiologists independently reviewed image quality using a 5-point scale (1: nondiagnostic-5: excellent).

Results: There was no difference in contrast dose, radiation dose, heart rate, and qualitative image quality between the two groups (all p > 0.05). However, Group A had higher mean enhancement in vessels (404 ± 66 HU) than Group B (321 ± 69 HU), and lower coefficients of variation of CT value in aortic sinus, right coronary artery, left anterior descending, and left circumflex (16.3%, 17.7%, 19.2%, and 20.5% vs 21.5%, 22.3%, 23.6%, and 22.9%, respectively), (all p < 0.05).

Conclusion: A new bolus tracking method enabled by free-breathing in CCTA on a 16 cm wide-coverage CT system increases the enhancement degree and uniformity in coronary arteries, compared with the conventional breath-holding method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.acra.2019.01.018DOI Listing
December 2019

The impact of self-reported preconception body mass index on gestational abnormal glucose tolerance in a Chinese center.

J Diabetes Complications 2018 Oct 3;32(10):951-954. Epub 2018 Aug 3.

Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University Health Science Center, China; Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, China. Electronic address:

Aims: To investigate the association between self-reported preconception body mass index (BMI) and the risk of abnormal glucose tolerance (AGT).

Methods: Data were obtained from a prospective cohort study conducted in China. We recruited 5305 qualified women who registered during 22-24 gestational weeks. Blood glucose was measured by trained professionals, and other health-related information was recorded prospectively. We used logistic regression to evaluate the relationship between preconception BMI with AGT and its subtypes, after controlling for potential confounders.

Results: 649 of the 5305 participants (12.2%) were diagnosed with AGT. The prevalences of AGT in underweight, normal weight, overweight and obese population indicated a significant linear increased trend (8.4%, 11.1%, 20.0% and 27.7%, respectively) (p < 0.001), regardless of parity status. After adjustment for maternal age, education and parity, the adjusted odds ratios of AGT for underweight: OR = 0.82 (95% CI: 0.62, 1.06); overweight: OR = 1.92 (95% CI: 1.54, 2.38); obese: OR = 2.82 (95% CI: 1.88, 4.22) compared with normal weight. Stratified analysis showed preconception BMI had a greater impact on primiparous women.

Conclusions: Our results support an association between self-reported preconception BMI with increased risk of AGT, and it was dependent on parity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jdiacomp.2018.07.009DOI Listing
October 2018

Nanostructured lipid carriers co-delivering lapachone and doxorubicin for overcoming multidrug resistance in breast cancer therapy.

Int J Nanomedicine 2018 12;13:4107-4119. Epub 2018 Jul 12.

Department of General Surgery 2, The Fourth People's Hospital of Jinan, Jinan, Shandong, China,

Background: Multidrug resistance is responsible for the poor outcome in breast cancer therapy. Lapa is a novel therapeutic agent that generates ROS through the catalysis of the NAD(P) H:quinone oxidoreductase-1 (NQO1) enzyme which significantly facilitate the intracellular accumulation of the co-delivered DOX to overcome MDR in cancer cells.

Purpose: Herein, in our study, nanostructured lipid carrier (NLC) co-delivering β-lapachone (Lapa) and doxorubicin (DOX) was developed (LDNLC) with the aim to overcome the multidrug resistance (MDR) in breast cancer therapy.

Patients And Methods: Lapa and DOX were loaded into NLC to prepare LDNLC using melted ultrasonic dispersion method.

Results: The well designed LDNLC was nanoscaled particles that exhibited preferable stability in physiological environment. In vitro cell experiments on MCF-7 ADR cells showed increased DOX retention as compared to DOX mono-delivery NLC (DNLC). In vivo anti-cancer assays on MCF-7 ADR tumor bearing mice model also revealed significantly enhanced efficacy of LDNLC than mono-delivery NLCs (DNLC and LNLC).

Conclusion: LDNLC might be a promising platform for effective breast cancer therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S163929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047616PMC
August 2018
-->