Publications by authors named "Xiaoqian Deng"

20 Publications

  • Page 1 of 1

Bioprinting of dual ECM scaffolds encapsulating limbal stem/progenitor cells in active and quiescent statuses.

Biofabrication 2021 08 13;13(4). Epub 2021 Aug 13.

Department of NanoEngineering, University of California San Diego, La Jolla, CA 92093, United States of America.

Limbal stem cell deficiency and corneal disorders are among the top global threats for human vision. Emerging therapies that integrate stem cell transplantation with engineered hydrogel scaffolds for biological and mechanical support are becoming a rising trend in the field. However, methods for high-throughput fabrication of hydrogel scaffolds, as well as knowledge of the interaction between limbal stem/progenitor cells (LSCs) and the surrounding extracellular matrix (ECM) are still much needed. Here, we employed digital light processing (DLP)-based bioprinting to fabricate hydrogel scaffolds encapsulating primary LSCs and studied the ECM-dependent LSC phenotypes. The DLP-based bioprinting with gelatin methacrylate (GelMA) or hyaluronic acid glycidyl methacrylate (HAGM) generated microscale hydrogel scaffolds that could support the viability of the encapsulated primary rabbit LSCs (rbLSCs) in culture. Immunocytochemistry and transcriptional analysis showed that the encapsulated rbLSCs remained active in GelMA-based scaffolds while exhibited quiescence in the HAGM-based scaffolds. The primary human LSCs encapsulated within bioprinted scaffolds showed consistent ECM-dependent active/quiescent statuses. Based on these results, we have developed a novel bioprinted dual ECM 'Yin-Yang' model encapsulating LSCs to support both active and quiescent statues. Our findings provide valuable insights towards stem cell therapies and regenerative medicine for corneal reconstruction.
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http://dx.doi.org/10.1088/1758-5090/ac1992DOI Listing
August 2021

Functional characterization of the transcription factors AhR and ARNT in Nilaparvata lugens.

Pestic Biochem Physiol 2021 Jul 14;176:104875. Epub 2021 May 14.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address:

In the present study, the aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) of Nilaparvata lugens were cloned and identified. The NlAhR and NlARNT expression levels significantly increased after imidacloprid, etofenprox and isoprocarb treatments. Knockdowns of NlAhR and NlARNT increased the susceptibility of N. lugens to imidacloprid, etofenprox and isoprocarb, and the detoxification enzyme activities were also significantly decreased. In addition, NlCYP301A1, NlGSTt1 and NlCarE7 were significantly down-regulated after injections of dsNlAhR and dsNlARNT, with the NlCarE7 expression decreasing by greater than 80%. Moreover, after knocking down NlCarE7, the susceptibility of N. lugens to etofenprox and isoprocarb significantly increased. Both NlAhR and NlARNT bound the NlCarE7 promoter and significantly enhanced the transcriptional activity. Our research revealed the functional roles of transcription factors NlAhR and NlARNT in the detoxification metabolism of N. lugens. The results provide a theoretical basis for the pest management and comprehensive control of N. lugens and increase our knowledge of insect toxicology.
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http://dx.doi.org/10.1016/j.pestbp.2021.104875DOI Listing
July 2021

Surface plasmon resonances boost the transverse magneto-optical Kerr effect in a CoFeB slab covered by a subwavelength gold grating for highly sensitive detectors.

Opt Express 2021 Mar;29(7):10546-10555

Herein, we have theoretically investigated the sensing performance-including enormous increase in the sensitivity and figure of merit (FOM)-of a magneto-optical surface plasmon resonance (MOSPR) sensor, which is based on the transverse magneto-optical Kerr effect (T-MOKE) in a ferromagnet coupled with a noble-metal grating. Specifically, we propose to use a CoFeB magnetic slab covered by a subwavelength, periodic gold grating configured as a magnetoplasmonic heterostructure. In such a device, sharp, Fano-like T-MOKE signals of high amplitude can be achieved due to the surface plasmon resonances (SPRs) excited in the presence of the gold grating, especially after optimizing the grating period. Tiny changes in the refractive index of an analyte surrounding the MOSPR sensor can be measured by analyzing the shift in the angle of incidence of the resonance positions of the T-MOKE signals. By calculating these resonance positions, we have demonstrated that it is possible to achieve a considerable sensitivity of 105° RIU and a FOM as high as ∼10. Such a MOSPR sensing system can be exploited in biosensors with high detection limits.
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http://dx.doi.org/10.1364/OE.414749DOI Listing
March 2021

Source apportionment of heavy metals in sediments of the urban rivers flowing into Haizhou Bay, Eastern China: using multivariate statistical analyses and Pb-Sr isotope fingerprints.

Environ Sci Pollut Res Int 2021 Jul 10;28(27):36354-36366. Epub 2021 Mar 10.

School of Marine Sciences, Nanjing University of Information Science & Technology, Nanjing, 210044, China.

Urban river runoff carrying various anthropogenic sources of heavy metals (HMs) is the most important input pathways for HM pollutions in the coastal region, apportioning sources of environmental pollutants is key to controlling coastal HM pollution. In the study, surface sediments were collected from seven urban rivers flowing through Lianyungang City and discharging into Haizhou Bay, Eastern China. The concentrations of HMs of the river sediments were, in mg/kg (mean value ± standard deviation): Mn (550 ± 227) > Zn (67 ± 61) > Cr (33 ± 12) > Ni (21 ± 8.5) > Cu (16 ± 7.6) > Pb (15 ± 5.6) > Cd (0.11 ± 0.06), which were slightly to moderately polluted. As important outlets for municipal and industrial sewages, the Shawang River and Linhong River were the most polluted. Based on the multivariate statistical analysis, HMs were attributed to anthropogenic source (industrial, domestic, and agricultural discharges) and natural source (soil parent materials and atmospheric deposition). Based on isotope source apportionment, Pb was mainly from natural source, exhausts of leaded gasoline vehicles, and coal combustion, with the mean contributions of 39.3%, 23.7%, and 37.0%, respectively, and Sr originated from natural source and anthropogenic source, with mean contributions of 31.8% and 68.2%, respectively. Pb-Sr isotopes illustrated that anthropogenic inputs were the dominant source for HMs in urban river sediments flowing into Haizhou Bay, and the isotope tracing results make up the discriminating deficiency of the multivariate statistical analysis.
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http://dx.doi.org/10.1007/s11356-021-13287-wDOI Listing
July 2021

Exfoliated Graphite Nanosheets Coating on Nano-grained SnO/LiTiO as a High-Performance Anode Material for Lithium-Ion Batteries.

Langmuir 2020 Dec 24;36(48):14666-14675. Epub 2020 Nov 24.

School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China.

The SnO/LiTiO/C compound was gained via hydrothermal, sintering, and ball milling methods. Nano-grained SnO/LiTiO are homogeneously wrapped in a sheet-like graphite. LiTiO possesses cyclic stability and superior rate capacity. Meanwhile, the SnO/LiTiO hybrid can supply abundant active sites for absorption of Li, mitigate chemical stress in cycling, and prevent the ultrathin graphite nanosheets from stacking. Besides, the sheet-like graphite could reduce volume variation in cycling and reduce transmission distance for the electron or Li. Therefore, an outstanding electrochemical property of the SnO/LiTiO/C composite can be obtained.
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http://dx.doi.org/10.1021/acs.langmuir.0c02524DOI Listing
December 2020

Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.

Biomaterials 2021 01 23;267:120462. Epub 2020 Oct 23.

Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address:

Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither an efficient culture of CjSCs nor the development of a minimally invasive ocular surface CjSC transplantation therapy has been reported. Here, we developed a robust in vitro expansion method for primary rabbit-derived CjSCs and applied digital light processing (DLP)-based bioprinting to produce CjSC-loaded hydrogel micro-constructs for injectable delivery. Expansion medium containing small molecule cocktail generated fast dividing and highly homogenous CjSCs for more than 10 passages in feeder-free culture. Bioprinted hydrogel micro-constructs with tunable mechanical properties enabled the 3D culture of CjSCs while supporting viability, stem cell phenotype, and differentiation potency into conjunctival goblet cells. These hydrogel micro-constructs were well-suited for scalable dynamic suspension culture of CjSCs and were successfully delivered to the bulbar conjunctival epithelium via minimally invasive subconjunctival injection. This work integrates novel cell culture strategies with bioprinting to develop a clinically relevant injectable-delivery approach for CjSCs towards the stem cell therapies for the treatment of ocular surface diseases.
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http://dx.doi.org/10.1016/j.biomaterials.2020.120462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719077PMC
January 2021

Sevoflurane modulates breast cancer cell survival via modulation of intracellular calcium homeostasis.

BMC Anesthesiol 2020 09 29;20(1):253. Epub 2020 Sep 29.

Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, 305 John Morgan Building, 3610 Hamilton Walk, Philadelphia, PA, 19104, USA.

Background: Some retrospective and in vitro studies suggest that general anesthetics influence breast cancer recurrence and metastasis. We compared the effects of general anesthetics sevoflurane versus propofol on breast cancer cell survival, proliferation and invasion in vitro. The investigation focused on effects in intracellular Ca homeostasis as a mechanism for general anesthetic-mediated effects on breast cancer cell survival and metastasis.

Methods: Estrogen receptor-positive (MCF7) and estrogen receptor-negative (MDA-MB-436) human breast cancer cell lines along with normal breast tissue (MCF10A) were used. Cells were exposed to sevoflurane or propofol at clinically relevant and extreme doses and durations for dose- and time-dependence studies. Cell survival, proliferation and migration following anesthetic exposure were assessed. Intracellular and extracellular Ca concentrations were modulated using Ca chelation and a TRPV1 Ca channel antagonist to examine the role of Ca in mediating anesthetic effects.

Results: Sevoflurane affected breast cancer cell survival in dose-, time- and cell type-dependent manners. Sevoflurane, but not propofol, at equipotent and clinically relevant doses (2% vs. 2 μM) for 6 h significantly promoted breast cell survival in all three types of cells. Paradoxically, extreme exposure to sevoflurane (4%, 24 h) decreased survival in all three cell lines. Chelation of cytosolic Ca dramatically decreased cell survival in both breast cancer lines but not control cells. Inhibition of TRPV1 receptors significantly reduced cell survival in all cell types, an effect that was partially reversed by equipotent sevoflurane but not propofol. Six-hour exposure to sevoflurane or propofol did not affect cell proliferation, metastasis or TRPV1 protein expression in any type of cell.

Conclusion: Sevoflurane, but not propofol, at clinically relevant concentrations and durations, increased survival of breast cancer cells in vitro but had no effect on cell proliferation, migration or TRPV1 expression. Breast cancer cells require higher cytoplasmic Ca levels for survival than normal breast tissue. Sevoflurane affects breast cancer cell survival via modulation of intracellular Ca homeostasis.
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http://dx.doi.org/10.1186/s12871-020-01139-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526115PMC
September 2020

Receptor-binding domain-specific human neutralizing monoclonal antibodies against SARS-CoV and SARS-CoV-2.

Signal Transduct Target Ther 2020 09 22;5(1):212. Epub 2020 Sep 22.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Fudan University, Shanghai, China.

The outbreaks of severe acute respiratory syndrome (SARS) and Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV and SARS-CoV-2, respectively, have posed severe threats to global public health and the economy. Treatment and prevention of these viral diseases call for the research and development of human neutralizing monoclonal antibodies (NMAbs). Scientists have screened neutralizing antibodies using the virus receptor-binding domain (RBD) as an antigen, indicating that RBD contains multiple conformational neutralizing epitopes, which are the main structural domains for inducing neutralizing antibodies and T-cell immune responses. This review summarizes the structure and function of RBD and RBD-specific NMAbs against SARS-CoV and SARS-CoV-2 currently under development.
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http://dx.doi.org/10.1038/s41392-020-00318-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7506210PMC
September 2020

Interleukin-6 promotes proliferative vitreoretinopathy by inducing epithelial-mesenchymal transition via the JAK1/STAT3 signaling pathway.

Mol Vis 2020 29;26:517-529. Epub 2020 Jul 29.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Purpose: Interleukin-6 (IL-6) is elevated in intraocular fluid from eyes with proliferative vitreoretinopathy (PVR), but the exact role of the cytokine is still unclear. We investigated the function and mechanism of IL-6 in retinal pigment epithelium (RPE) cell biology in vitro and in a mouse model in vivo.

Methods: After treatment with various concentrations of IL-6, RPE cell proliferation was assessed with cell counting kit-8 (CCK-8) assay, and epithelial-mesenchymal transition (EMT) markers were evaluated using western blotting and immunofluorescent staining. The activation of JAK1/STAT3 signaling was determined with western blotting. Moreover, the effects of blockade of IL-6/JAK1/STAT3 signaling were investigated using pharmacological inhibitor S3I-201. For in vivo studies, the PVR model was induced with intravitreal injection of dispase/collagenase in wild-type and IL-6 knockout mice. The severity of PVR was evaluated with histological analysis. The expression of IL-6, gp130, and EMT markers was assessed with quantitative real-time PCR and western blotting.

Results: IL-6 statistically significantly induced RPE cell proliferation and EMT in a dose-dependent manner in vitro which was accompanied by rapid phosphorylation of JAK1 and STAT3. Blockade of the IL-6/JAK1/STAT3 pathway with S3I-201 apparently inhibited RPE proliferation and EMT. Furthermore, IL-6 and gp130 overexpression, and JAK1/STAT3 signaling hyperactivation were detected in the retinas of the wild-type mice at 1, 3, and 7 days after dispase/collagenase injection. Finally, we confirmed that IL-6 deficiency markedly alleviated mouse PVR development via inhibiting EMT.

Conclusions: These findings indicate that IL-6 promotes PVR by inducing RPE proliferation and EMT via the JAK1/STAT3 signaling pathway. We provided new evidence that therapeutic strategies to block IL-6 may be beneficial for PVR.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406861PMC
July 2021

Influenza virus glycoprotein-reactive human monoclonal antibodies.

Microbes Infect 2020 Jul - Aug;22(6-7):263-271. Epub 2020 Jun 19.

College of Life Sciences, Hebei Agricultural University, Baoding, China. Electronic address:

Influenza continues to be a significant public health challenge. Two glycoproteins on the surface of influenza virus, hemagglutinin and neuraminidase, play a prominent role in the process of influenza virus infection and release. Monoclonal antibodies targeting glycoproteins can effectively prevent the spread of the virus. In this review, we summarized currently reported human monoclonal antibodies targeting glycoproteins of influenza A and B viruses.
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http://dx.doi.org/10.1016/j.micinf.2020.06.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303604PMC
May 2021

Wolbachia enhances expression of NlCYP4CE1 in Nilaparvata lugens in response to imidacloprid stress.

Insect Sci 2021 Apr 21;28(2):355-362. Epub 2020 Jul 21.

Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.

The brown planthopper, Nilaparvata lugens, is one of the main insect pests of rice. The N. lugens gene NlCYP4CE1 encodes cytochrome P450 monooxygenase (P450), which is a key enzyme in the metabolism of the insecticide imidacloprid. Previous research has suggested that the expression of NlCYP4CE1 is induced by imidacloprid stress, but the effect of bacterial symbionts on its expression has not been determined. The results of this study show that exposure to subtoxic imidacloprid changed the structure of the bacterial symbiont community in N. lugens. Specifically, the total bacterial content increased, but the bacterial species diversity significantly decreased. Wolbachia accounted for the largest proportion of bacteria in N. lugens; its abundance significantly increased after subtoxic imidacloprid exposure. The transcript level of NlCYP4CE1 was significantly increased by imidacloprid, but this effect was significantly weakened after Wolbachia was cleared with tetracycline. This result suggests that Wolbachia enhances the expression of NlCYP4CE1 to promote the detoxification metabolic response to imidacloprid stress. Understanding the effect of bacterial symbionts on gene expression in the host provides a new perspective on interactions between insecticides and their target insect pests, and highlights that subtoxic imidacloprid exposure may raise the risk of insecticide resistance by altering the structure of bacterial symbiont communities.
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http://dx.doi.org/10.1111/1744-7917.12834DOI Listing
April 2021

Development of small-molecule inhibitors against hantaviruses.

Microbes Infect 2020 Jul - Aug;22(6-7):272-277. Epub 2020 May 20.

College of Life Sciences, Hebei Agricultural University, Baoding, China. Electronic address:

Hantavirus (HV), a pathogen of animal infectious diseases that poses a threat to humans, has attracted extensive attention. Clinically, HV can cause hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), between which HFRS is mostly in Eurasia, and HPS is mostly in the Americas. This paper reviews the research progress of small-molecule inhibitors of HV.
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http://dx.doi.org/10.1016/j.micinf.2020.05.011DOI Listing
May 2021

Recent advances in the detection of respiratory virus infection in humans.

J Med Virol 2020 04 4;92(4):408-417. Epub 2020 Feb 4.

Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York.

Respiratory tract viral infection caused by viruses or bacteria is one of the most common diseases in human worldwide, while those caused by emerging viruses, such as the novel coronavirus, 2019-nCoV that caused the pneumonia outbreak in Wuhan, China most recently, have posed great threats to global public health. Identification of the causative viral pathogens of respiratory tract viral infections is important to select an appropriate treatment, save people's lives, stop the epidemics, and avoid unnecessary use of antibiotics. Conventional diagnostic tests, such as the assays for rapid detection of antiviral antibodies or viral antigens, are widely used in many clinical laboratories. With the development of modern technologies, new diagnostic strategies, including multiplex nucleic acid amplification and microarray-based assays, are emerging. This review summarizes currently available and novel emerging diagnostic methods for the detection of common respiratory viruses, such as influenza virus, human respiratory syncytial virus, coronavirus, human adenovirus, and human rhinovirus. Multiplex assays for simultaneous detection of multiple respiratory viruses are also described. It is anticipated that such data will assist researchers and clinicians to develop appropriate diagnostic strategies for timely and effective detection of respiratory virus infections.
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http://dx.doi.org/10.1002/jmv.25674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166954PMC
April 2020

Development of Small-Molecule Inhibitors Against Zika Virus Infection.

Front Microbiol 2019 6;10:2725. Epub 2019 Dec 6.

College of Life and Science, Hebei Agricultural University, Baoding, China.

In recent years, the outbreak of infectious disease caused by Zika virus (ZIKV) has posed a major threat to global public health, calling for the development of therapeutics to treat ZIKV disease. Here, we have described the different stages of the ZIKV life cycle and summarized the latest progress in the development of small-molecule inhibitors against ZIKV infection. We have also discussed some general strategies for the discovery of small-molecule ZIKV inhibitors.
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http://dx.doi.org/10.3389/fmicb.2019.02725DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909824PMC
December 2019

A cross-sectional study to assess the difference in perception of day surgery information between patients and medical staff in China.

Patient Prefer Adherence 2019 5;13:381-387. Epub 2019 Mar 5.

School of Information and Software Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan, People's Republic of China,

Purpose: The development of day surgery in China is still in its infancy. The aim of this study is to examine the difference in perception between patients and medical staff about day surgery and to find out what kind of information should be delivered to patients perioperatively.

Methods: The study was designed as a cross-sectional study using survey questionnaires. Patients undergoing day surgery and medical staff working in the day surgery center received a preoperative teaching questionnaire (PTQ). The PTQ consisted of preoperative, operative, postoperative and anesthesia information. Participants were asked to rate accurately what day surgery information they perceived as important on a five-point Likert-type scale that ranged from very important to not important. The main outcome was the value patients and staff place on perioperative information.

Results: For patients, details of anesthesia (82.6%) was the most important day surgery information, while for medical staff, preoperative (58.1%), postoperative (60.7%) and anesthesia (60.0%) information were of the same importance. Patients ranked surgery effect (83.3%) and pain management (82.5%) as two of the top five most important details about day surgery, but these were not listed in the top 5 rankings of staff. Student's -test results of mean rankings also showed that patients placed more importance on surgery effect (4.8±0.6, 4.6±0.6, =0.036) and pain management (4.8±0.5, 4.5±0.5, =0.031) than the medical staff did.

Conclusion: In China, day surgery is an emerging practice with both patients and medical practitioners still lacking experience, so high-quality perioperative teaching is necessary and important. Our study examining patients and staffs' views on day surgery information should be considered when developing perioperative teaching programs. To increase patient satisfaction of the day surgery experience, delivery of patient-specific information tailored to individual circumstances is necessary.
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http://dx.doi.org/10.2147/PPA.S196674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407508PMC
March 2019

Inhibition of astrocytic differentiation of transplanted neural stem cells by chondroitin sulfate methacrylate hydrogels for the repair of injured spinal cord.

Biomater Sci 2019 Apr;7(5):1995-2008

Department of Spine Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China.

Neural stem cell (NSC) transplantation exerts a therapeutic effect on spinal cord injury (SCI) but is limited to an unregulated differentiation pattern by which NSCs preferentially differentiate into astrocytes, with relatively few neurons. It is well established that the increased NSC-derived astrocytes exhibit aberrant axonal sprouting associated with allodynia-like symptoms of the forepaws. Some strategies have been used to overcome this issue, such as regulation of major pathways, ex vivo gene transfer, and genetic overexpression. However, lack of efficiency, viral vector safety issues and the risk of tumorigenesis have hindered the clinical application of these treatments. Here, we show that astrocytic differentiation of NSCs in vitro and in vivo can be inhibited by encapsulation of cells in a three-dimensional chondroitin sulfate methacrylate (CSMA) hydrogel. When CSMA hydrogels were used to transplant NSCs, the combinatory implant promoted functional recovery and attenuated the hypersensitivity responses of the forepaws. Further analysis showed that transplantation of NSCs within CSMA hydrogels reduced injured cavity areas and promoted neurogenesis rather than fibroglial formation after graft implantation. Furthermore, the treatment prevented allodynia-related CGRP/GAP43-positive nociception due to fibers sprouting into inappropriate lamina regions. Taken together, these findings show that CSMA/NSCs combined transplantation helps prevent adverse side effects of NSCs treatment and promotes recovery of SCI.
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http://dx.doi.org/10.1039/c8bm01363bDOI Listing
April 2019

Development of Small-Molecule MERS-CoV Inhibitors.

Viruses 2018 12 17;10(12). Epub 2018 Dec 17.

College of Life and Science, Hebei Agricultural University, Baoding 071001, China.

Middle East respiratory syndrome coronavirus (MERS-CoV) with potential to cause global pandemics remains a threat to the public health, security, and economy. In this review, we focus on advances in the research and development of small-molecule MERS-CoV inhibitors targeting different stages of the MERS-CoV life cycle, aiming to prevent or treat MERS-CoV infection.
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http://dx.doi.org/10.3390/v10120721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316138PMC
December 2018

Short message service (SMS) can enhance compliance and reduce cancellations in a sedation gastrointestinal endoscopy center: a prospective randomized controlled trial.

J Med Syst 2015 Jan 5;39(1):169. Epub 2014 Dec 5.

Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan Province, China.

Many outpatients who inadequately prepared for the procedure were cancelled on the day of the examination for various reasons. The aim of study was to investigate whether short message service (SMS) can improve patients' compliance and reduce cancellation rates. Outpatients scheduled for sedation gastrointestinal endoscopy were randomly assigned to mobile phone SMS group or control group. Patients in the control group received a leaflet on preparation instructions, while patients in the SMS group received SMS reminders after making an appointment. A total of 1786 patients were analyzed. There was a significant reduction in the rate of cancellations for patients in the SMS group (4.8%) compared with patients in the control group (8.0%) (P<0.001). Patients in the SMS group were 40% less likely to be cancelled by medical staff than patients in the control group. The compliance score of the two groups based on demographic and clinic characteristic distribution showed that for both male and female patients, the compliance score was higher in the SMS group than that in the control group (P=0.023, P<0.001, respectively). Additionally, the compliance score was also significantly higher in the SMS group among patients who were under 50 years old, less than an undergraduate education level, experiencing their first time for procedure, or whose procedures were gastroscopy, waiting time was between 4 and 15 days, and schedules were in morning (P≤0.032). SMS reminders can be considered a complement to conventional preparation instructions, which could help improve the compliance of outpatients and reduce the rate of cancellations.
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http://dx.doi.org/10.1007/s10916-014-0169-zDOI Listing
January 2015

A cross-sectional study to assess the feasibility of a short message service to improve adherence of outpatients undergoing sedation gastrointestinal endoscopy in the People's Republic of China.

Patient Prefer Adherence 2014 23;8:1293-7. Epub 2014 Sep 23.

Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, Sichuan, People's Republic of China Patient Preference and Adherence 2014:8 1293-1297.

Background: Adherence to the prescribed preparation regimen for patients scheduled for sedation gastrointestinal endoscopy (SGIE) often fails to meet health care providers' expectations. Therefore, the feasibility of using mobile phones to improve the adherence of outpatients was assessed among outpatients scheduled for SGIE.

Methods: The study was designed as a cross-sectional study using survey questionnaires. Outpatients who would be undergoing SGIE were enrolled. The main outcomes included proportions of outpatients who have access to mobile phones, and the use of text-messaging among outpatients. Willingness to receive text messages and personal opinions about short message service (SMS) were also investigated. Characteristics of outpatients, including socio-demographic factors, with a mobile phone (or those who could receive and read messages) compared with those without a mobile phone (or those who could not receive and read messages) were compared using the chi-square test. Logistic regression was used to analyze significant contributing factors associated with mobile phone ownership and the use of text messages.

Results: A large majority of outpatients owned personal mobile phones (94.9%) and could receive and read the messages (78.9%). Most of the outpatients were willing to receive a pre-procedure education via text message (81.9%). Outpatients aged 16-39 years old and those with a college education or higher were more likely to use mobile phones and read SMS than outpatients aged 60-80 years old and those with only a primary education level (P<0.0001). Outpatients who lived in an urban setting were more likely to own a mobile phone than outpatients who lived in a rural setting (P=0.002).

Conclusion: The accessibility of mobile phones and the frequent use of message functions on mobile phones provide support for the initiation of an SMS reminder system for outpatients who will undergo SGIE in the People's Republic of China. In addition, the characteristics of the target study population should be considered when carrying out an SMS intervention.
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http://dx.doi.org/10.2147/PPA.S68814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4179828PMC
October 2014

Clinical comparison of propofol-remifentanil TCI with sevoflurane induction/maintenance anesthesia in laparoscopic cholecystectomy.

Pak J Med Sci 2014 Sep;30(5):1017-21

Tao Zhu, MD, Professor, Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

Unlabelled: Objective : We aimed to compare the anesthetic characteristics between total intravenous anesthesia (TIVA) using propofol-remifentanil with target control infusion (TCI) and volatile induction and maintenance anesthesia (VIMA) using sevoflurane and sufentanyl for patients undergoing laparoscopic cholecystectomy.

Methods: A total of 120 patients undergoing laparoscopic cholecystectomy were randomly assigned to two groups. Patients in group T received TCI of propofol-remifentanil for induction and maintenance. Patients in group S received sevoflurane-sufentanyl for induction and maintenance.

Results: Patients in group S had a significantly faster induction time than patients in group T (109s vs.44s). The emergence time in terms of time to extubation was comparable between the two groups, while the time to eyes opening (419s vs.483s, p=0.006) and duration in PACU were longer in group S (44 min vs.53 min, p=0.017). Ten (17.2%) patients in group S were administered an antihypertensive drug when gallbladder issues were present, while only 1(1.7%) patient needed this drug in group T (p=0.004).More patients in group T than in group S received fentanyl for analgesia in PACU (88%vs.70%, p=0.013). The incidence of postoperative nausea and vomiting (PONV) in PACU was higher in group S than in group T (20% vs.38%, p=0.027).

Conclusion: Both techniques had advantages and disadvantages in laparoscopic cholecystectomy; none of the techniques studied was superior.
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http://dx.doi.org/10.12669/pjms.305.5196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4163224PMC
September 2014
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