Publications by authors named "Xiaoqi Yu"

55 Publications

Compensatory role of endogenous sulfur dioxide in nitric oxide deficiency-induced hypertension.

Redox Biol 2021 Nov 18;48:102192. Epub 2021 Nov 18.

Department of Pediatrics, Peking University First Hospital, Beijing, China. Electronic address:

Objective: This study aimed to determine the communicational pattern of gaseous signaling molecules sulfur dioxide (SO) and nitric oxide (NO) between vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs), and elucidate the compensatory role and significance of endogenous SO in the development of hypertension due to NO deficiency.

Approach And Results: Blood pressure was monitored by the tail-cuff and implantable physiological signal telemetry in L-nitro-arginine methyl ester (l-NAME)-induced hypertensive mice, and structural alterations of mouse aortic vessels were detected by the elastic fiber staining method. l-NAME-treated mice showed decreased plasma NO levels, increased SO levels, vascular remodeling, and increased blood pressure, and application of l-aspartate-β-hydroxamate, which inhibits SO production, further aggravated vascular structural remodeling and increased blood pressure. Moreover, in a co-culture system of HAECs and HASMCs, NO from HAECs did not influence aspartate aminotransferase (AAT)1 protein expression but decreased AAT1 activity in HASMCs, thereby resulting in the inhibition of endogenous SO production. Furthermore, NO promoted S-nitrosylation of AAT1 protein in HASMCs and purified AAT1 protein. Liquid chromatography with tandem mass spectrometry showed that the Cys192 site of AAT1 purified protein was modified by S-nitrosylation. In contrast, dithiothreitol or C192S mutations in HASMCs blocked NO-induced AAT1 S-nitrosylation and restored AAT1 enzyme activity.

Conclusion: Endothelium-derived NO inhibits AAT activity by nitrosylating AAT1 at the Cys192 site and reduces SO production in HASMCs. Our findings suggest that SO acts as a compensatory defense system to antagonize vascular structural remodeling and hypertension when the endogenous NO pathway is disturbed.
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http://dx.doi.org/10.1016/j.redox.2021.102192DOI Listing
November 2021

Inactivated SARS-CoV-2 vaccine does not influence the profile of prothrombotic antibody nor increase the risk of thrombosis in a prospective Chinese cohort.

Sci Bull (Beijing) 2021 Nov 27;66(22):2312-2319. Epub 2021 Jul 27.

Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

The presence of antiphospholipid antibodies was shown to be associated with thrombosis in coronavirus disease 2019 (COVID-19) patients. Recently, according to reports from several studies, the vaccine-induced immune thrombotic thrombocytopenia is mediated by anti-platelet factor 4 (PF4)-polyanion complex in adenovirus-vectored COVID-19 vaccine recipients. It is impendent to explore whether inactivated COVID-19 vaccine widely used in China influences prothrombotic autoantibody production and induces thrombosis. In this prospective study, we recruited 406 healthcare workers who received two doses, 21 days apart, of inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine (BBIBP-CorV, Sinopharm). Paired blood samples taken before vaccination and four weeks after the second vaccination were used in detecting prothrombotic autoantibodies, including anticardiolipin (aCL), anti-β2 glycoprotein I (aβ2GP1), anti-phosphatidylserine/prothrombin (aPS/PT), and anti-PF4-heparin. The seroconversion rate of SARS-CoV-2 specific antibodies was 95.81% (389/406) four weeks after vaccination. None of the subjects had spontaneous thrombosis or thrombocytopenia over a minimum follow-up period of eight weeks. There was no significant difference in the presence of all ten autoantibodies between samples collected before and after vaccination: for aCL, IgG (7 vs. 8,  = 0.76), IgM (41 vs. 44,  = 0.73), IgA (4 vs. 4,  = 1.00); anti-β2GP1, IgG (7 vs. 6,  = 0.78), IgM (6 vs. 5,  = 0.76), IgA (3 vs. 5,  = 0.72); aPS/PT IgG (0 vs. 0,  = 1.00), IgM (6 vs. 5,  = 0.76); aPF4-heparin (2 vs. 7,  = 0.18), and antinuclear antibody (ANA) (18 vs. 21,  = 0.62). Notably, seven cases presented with anti-PF4-heparin antibodies (range: 1.18-1.79 U/mL) after vaccination, and none of them exhibited any sign of thrombotic disorder. In conclusion, inactivated SARS-CoV-2 vaccine does not influence the profile of antiphospholipid antibody and anti-PF4-heparin antibody nor increase the risk of thrombosis.
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http://dx.doi.org/10.1016/j.scib.2021.07.033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313791PMC
November 2021

High Level of Soluble CD146 In Cerebrospinal Fluid Might be a Biomarker of Severity of Anti-N-Methyl-D-Aspartate Receptor Encephalitis.

Front Immunol 2021 16;12:680424. Epub 2021 Jun 16.

Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Disruption of the blood-brain barrier (BBB) is an important pathophysiological process of anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis. A recent multi-center study showed that soluble (s) CD146 is a potential biomarker for monitoring early BBB damage and central nervous system inflammation, but little is known about sCD146 in anti-NMDAR encephalitis.

Method: Twenty-three anti-NMDAR encephalitis patients and seventeen controls with non-inflammatory neurological diseases were recruited. sCD146 and inflammatory cytokines in cerebrospinal fluid (CSF) and serum were detected by ELISA. Modified Rankin scale (mRS) scores were used to assess the neurological status of each patient. A follow-up review was completed three months after discharge.

Results: sCD146 levels in the CSF of patients with the acute stage anti-NMDAR encephalitis were significantly increased compared with controls and accompanied by increases in TNF-α, IL-6 and IL-10. CSF sCD146 was positively correlated with neuroinflammatory factors in the CSF and with mRS score. Three months after effective treatment, CSF sCD146 in patients was significantly decreased but remained significantly different compared with the controls.

Conclusion: Our data suggested that higher expression of CSF sCD146 correlated with more serious neurological damage. Therefore, levels of CSF sCD146 may represent a promising indicator for monitoring disease and optimizing clinical treatment decisions in the early stages of anti-NMDAR encephalitis.
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http://dx.doi.org/10.3389/fimmu.2021.680424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245058PMC
October 2021

Semiquantitative Visual Chiral Assay with a Pseudoenantiomeric Fluorescent Sensor Pair.

J Org Chem 2021 07 24;86(14):9603-9609. Epub 2021 Jun 24.

Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States.

A new red-light-emitting fluorescent probe ()- was synthesized. In the presence of Zn, this compound was found to exhibit good enantioselective fluorescence enhancement at λ = 655 nm when treated with a variety of amino acids in aqueous solution. This probe in combination with a green-light-emitting probe ()- that has enantioselective fluorescence enhancement at λ = 505 nm has formed a pseudoenantiomeric sensor pair because of their opposite enantioselectivities. This sensor pair can simultaneously detect both enantiomers of a chiral amino acid at two very different wavelengths (Δ = 150 nm). It was used to visually and semiquantitatively determine the enantiomeric compositions of amino acids. For example, when a 1:1 mixture of ()- and ()- was treated with Zn(OAc) and histidine samples of 0-100% [d-His], the color of the mixtures changed from green to yellow, orange, and red under a UV lamp (365 nm), which allowed a quick quantification of [d-His]%. This is the first example of using fluorescence to visually quantify the enantiomeric composition of chiral compounds.
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http://dx.doi.org/10.1021/acs.joc.1c00875DOI Listing
July 2021

From MonoBINOL to BisBINOL: Expanded Enantioselective Fluorescent Recognition of Amino Acids.

J Org Chem 2021 05 26;86(9):6780-6786. Epub 2021 Apr 26.

Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, Virginia 22904, United States.

Condensation of the methoxymethyl-protected ()-3,3'-diformyl-1,1'-bi-2-naphthol (BINOL) with (pyridine-2,6-diylbis(methylene))bis(triphenyl phosphonium)dibromide in the presence of a base followed by deprotection gave a new bisBINOL-based fluorescent probe (,)-. This compound showed expanded substrate scope in the recognition of amino acids with good enantioselective fluorescence responses toward 17 common amino acids. Two diastereomeric imines were synthesized from the condensation of (,)- with l- and d-valine, and the reactions of these imines with Zn(OAc) were investigated by various spectroscopic methods for a better understanding of the enantioselective fluorescent recognition process.
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http://dx.doi.org/10.1021/acs.joc.1c00507DOI Listing
May 2021

A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa.

J Mater Chem B 2021 05;9(17):3662-3665

Department of Obstetrics/Gynecology, Joint Laboratory of Reproductive Medicine, SCU-CUHK, Key Laboratory of Obstetric, Gynaecologic and Paediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, China. and Reproductive Endocrinology and Regulation Laboratory, West China Second University Hospital, Sichuan University, Chengdu, 610041, China.

A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm.
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http://dx.doi.org/10.1039/d1tb00108fDOI Listing
May 2021

Endogenous SO-dependent Smad3 redox modification controls vascular remodeling.

Redox Biol 2021 05 18;41:101898. Epub 2021 Feb 18.

Department of Pediatrics, Peking University First Hospital, Beijing, 100034, China; Key Laboratory of Cardiovascular Sciences, Ministry of Education, China. Electronic address:

Sulfur dioxide (SO) has emerged as a physiological relevant signaling molecule that plays a prominent role in regulating vascular functions. However, molecular mechanisms whereby SO influences its upper-stream targets have been elusive. Here we show that SO may mediate conversion of hydrogen peroxide (HO) to a more potent oxidant, peroxymonosulfite, providing a pathway for activation of HO to convert the thiol group of protein cysteine residues to a sulfenic acid group, aka cysteine sulfenylation. By using site-centric chemoproteomics, we quantified >1000 sulfenylation events in vascular smooth muscle cells in response to exogenous SO. Notably, ~42% of these sulfenylated cysteines are dynamically regulated by SO, among which is cysteine-64 of Smad3 (Mothers against decapentaplegic homolog 3), a key transcriptional modulator of transforming growth factor β signaling. Sulfenylation of Smad3 at cysteine-64 inhibits its DNA binding activity, while mutation of this site attenuates the protective effects of SO on angiotensin II-induced vascular remodeling and hypertension. Taken together, our findings highlight the important role of SO in vascular pathophysiology through a redox-dependent mechanism.
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http://dx.doi.org/10.1016/j.redox.2021.101898DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933484PMC
May 2021

New Insights into the Role of Ferritin in Iron Homeostasis and Neurodegenerative Diseases.

Mol Neurobiol 2021 Jun 28;58(6):2812-2823. Epub 2021 Jan 28.

Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders, School of Basic Medicine, Qingdao University, Qingdao, 266071, China.

Growing evidence has indicated that iron deposition is one of the key factors leading to neuronal death in the neurodegenerative diseases. Ferritin is a hollow iron storage protein composed of 24 subunits of two types, ferritin heavy chain (FTH) and ferritin light chain (FTL), which plays an important role in maintaining iron homeostasis. Recently, the discovery of extracellular ferritin and ferritin in exosomes indicates that ferritin might be not only an iron storage protein within the cell, but might also be an important factor in the regulation of tissue and body iron homeostasis. In this review, we first described the structural characteristics, regulation and the physiological functions of ferritin. Secondly, we reviewed the current evidence concerning the mechanisms underlying the secretion of ferritin and the possible role of secreted ferritin in the brain. Then, we summarized the relationship between ferritin and the neurodegenerative diseases including Parkinson's disease (PD), Alzheimer's disease (AD) and neuroferritinopathy (NF). Given the importance and relationship between iron and neurodegenerative diseases, understanding the role of ferritin in the brain can be expected to contribute to our knowledge of iron dysfunction and neurodegenerative diseases.
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http://dx.doi.org/10.1007/s12035-020-02277-7DOI Listing
June 2021

Ndfip1 Prevents Rotenone-Induced Neurotoxicity and Upregulation of α-Synuclein in SH-SY5Y Cells.

Front Mol Neurosci 2020 5;13:613404. Epub 2021 Jan 5.

Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Department of Physiology, Medical College of Qingdao University, Qingdao, China.

Nedd4 family interacting protein 1 (Ndfip1) is an adaptor of Nedd4-family ubiquitin ligases. Experimental results showed that Ndfip1 had a potential neuroprotective effect in neurology diseases. However, the neuroprotective effect and the underlying mechanisms of Ndfip1 in Parkinson's disease (PD) have not yet been fully elucidated. Therefore, in this study, we explored the neuroprotective effect of Ndfip1 against mitochondrial complex I inhibitor rotenone in a human dopaminergic neuroblastoma SH-SY5Y cell line and further elucidated its possible underlying mechanisms. Our results showed that rotenone could induce the up-regulation of α-synuclein (α-syn) in both mRNA and protein levels. The expression of Ndfip1 decreased at 24 h after rotenone treatment. Further study showed that high expression of Ndfip1 could protect SH-SY5Y cells against rotenone-induced neurotoxicity and antagonize the rotenone-induced increase in α-syn protein levels. In addition, high expression of Ndfip1 inhibited rotenone-induced increase in the protein levels of caspase-3 and decrease in tyrosine hydroxylase (TH). Further study showed that Ndfip1 did not affect the protein expression of iron regulatory protein 1 (IRP1), transferrin receptor 1 (TfR1), while antagonized the increase in protein levels of P62 and ferritin L caused by rotenone. Our findings provide specific identification of Ndfip1 proteins to inhibit the increase of α-syn in rotenone-induced SH-SY5Y cells. Ndfip1 might be a new theoretical drug target for the prevention and treatment of PD.
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http://dx.doi.org/10.3389/fnmol.2020.613404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7813998PMC
January 2021

Disruption of EARLY LESION LEAF 1, encoding a cytochrome P450 monooxygenase, induces ROS accumulation and cell death in rice.

Plant J 2021 02 20;105(4):942-956. Epub 2020 Dec 20.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, P. R. China.

Lesion-mimic mutants (LMMs) provide a valuable tool to reveal the molecular mechanisms determining programmed cell death (PCD) in plants. Despite intensive research, the mechanisms behind PCD and the formation of lesions in various LMMs still remain to be elucidated. Here, we identified a rice (Oryza sativa) LMM, early lesion leaf 1 (ell1), cloned the causal gene by map-based cloning, and verified this by complementation. ELL1 encodes a cytochrome P450 monooxygenase, and the ELL1 protein was located in the endoplasmic reticulum. The ell1 mutant exhibited decreased chlorophyll contents, serious chloroplast degradation, upregulated expression of chloroplast degradation-related genes, and attenuated photosynthetic protein activity, indicating that ELL1 is involved in chloroplast development. RNA sequencing analysis showed that genes related to oxygen binding were differentially expressed in ell1 and wild-type plants; histochemistry and paraffin sectioning results indicated that hydrogen peroxide (H O ) and callose accumulated in the ell1 leaves, and the cell structure around the lesions was severely damaged, which indicated that reactive oxygen species (ROS) accumulated and cell death occurred in the mutant. TUNEL staining and comet experiments revealed that severe DNA degradation and abnormal PCD occurred in the ell1 mutants, which implied that excessive ROS accumulation may induce DNA damage and ROS-mediated cell death in the mutant. Additionally, lesion initiation in the ell1 mutant was light dependent and temperature sensitive. Our findings revealed that ELL1 affects chloroplast development or function, and that loss of ELL1 function induces ROS accumulation and lesion formation in rice.
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http://dx.doi.org/10.1111/tpj.15079DOI Listing
February 2021

Chemoselective and enantioselective fluorescent recognition of glutamic and aspartic acids.

Chem Commun (Camb) 2020 Dec;56(95):15012-15015

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, China.

A highly chemoselective as well as enantioselective fluorescent probe has been discovered for the recognition of the acidic amino acids, including glutamic acid and aspartic acid. This study has established a novel amino acid recognition mechanism by an aldehyde-based fluorescent probe.
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http://dx.doi.org/10.1039/d0cc06736aDOI Listing
December 2020

LRG1 maintains sterile lemma identity by regulating OsMADS6 expression in rice.

Sci China Life Sci 2021 Jul 30;64(7):1190-1192. Epub 2020 Oct 30.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.

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http://dx.doi.org/10.1007/s11427-020-1816-xDOI Listing
July 2021

Characterization of antibody responses to SARS-CoV-2 in convalescent COVID-19 patients.

J Med Virol 2021 04 10;93(4):2227-2233. Epub 2020 Nov 10.

Research Laboratory of Clinical Virology, Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The coronavirus disease 2019 (COVID-19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, little is known about the durability of the antibody response during COVID-19 convalescent phase. We investigated the prevalence of anti-SARS-CoV-2 specific antibodies including immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies and the dynamic changes in antibody levels in convalescent COVID-19 patients. A total of 159 blood samples were collected from 52 recovered COVID-19 patients up to six months after symptom onset for longitudinal serological tests. The positive rate of IgG and IgM antibodies was 92.3% and 90.4% in the first month after symptom onset, and the seropositivity of IgG antibody remained high at all follow-up time points, whereas the seropositivity of IgM antibody decreased to 22.73% by the sixth months after symptom onset. The level of IgG antibody was stable, the level of IgM antibody decreased slightly in the early convalescent phase and was detected in only five patients in the sixth month after symptom onset. The level of IgG antibody was higher in the severe and critical group than in the moderate group. The anti-SARS-CoV-2 specific antibodies have a long-term persistence in convalescent COVID-19 patients, whether they have long-term protection need to be further investigated.
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http://dx.doi.org/10.1002/jmv.26646DOI Listing
April 2021

The Psychological Health Status of Healthcare Workers During the COVID-19 Outbreak: A Cross-Sectional Survey Study in Guangdong, China.

Front Public Health 2020 18;8:562885. Epub 2020 Sep 18.

Department of Geriatric Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.

The sudden outbreak of COVID-19 has caused mental stress on healthcare workers (HCW). This study aimed to assess their psychological health status at the peak of COVID-19 and to identify some coping strategies. A cross-sectional survey study was conducted during the outbreak of COVID-19. The survey was completed by 908/924 HCW (response rate 98.27%) in government-designated hospitals in Guangdong, China. A quality of life (QoL) scale, the Zung Self-Rating Anxiety Scale (SAS), and the Zung Self-Rating Depression Scale (SDS) were used to evaluate their psychological status. Logistic regression models were used to identify the occupational factors related to anxiety or depression. A total of 221 (24.34%) respondents had varying levels of anxiety, and 299 (32.93%) of them had depression. The mean SAS (42.9) and SDS (47.8) scores of HCW indicated that they were in the normal range for both anxiety and depression. Contact with COVID-19 cases or suspected cases, worry about suffering from COVID-19, worry about their family, and dismission during the COVID-19 period were significant work-related contributing factors to the psychological health problems of HCW (all p<0.01). The overall psychological health status of HCW in Guangdong, China, during the outbreak of COVID-19 was not overly poor. Updating and strengthening training in disease information, the provision of adequate medical supplies, and care about the life and health of medical staff and their family members may reduce their mental stress.
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http://dx.doi.org/10.3389/fpubh.2020.562885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7530179PMC
May 2021

Colyliform Crystalline 2D Covalent Organic Frameworks (COFs) with Quasi-3D Topologies for Rapid I Adsorption.

Angew Chem Int Ed Engl 2020 Dec 2;59(50):22697-22705. Epub 2020 Oct 2.

College of Chemistry, Sichuan University, Key Laboratory of Radiation Physics & Technology, Ministry of Education, No. 29 Wangjiang Road, Chengdu, 610064, P. R. China.

Constructing three-dimensional (3D) structural characteristics on two-dimensional (2D) covalent organic frameworks (COFs) is a good approach to effectively improve the permeability and mass transfer rate of the materials and realize the rapid adsorption for guest molecules, while avoiding the high cost and monomer scarcity in preparing 3D COFs. Herein, we report for the first time a series of colyliform crystalline 2D COFs with quasi-three-dimensional (Q-3D) topologies, consisting of unique "stereoscopic" triangular pores, large interlayer spacings and flexible constitutional units which makes the pores elastic and self-adaptable for the guest transmission. The as-prepared QTD-COFs have a faster adsorption rate (2.51 g h ) for iodine than traditional 2D COFs, with an unprecedented maximum adsorption capacity of 6.29 g g . The excellent adsorption performance, as well as the prominent irradiation stability allow the QTD-COFs to be applied for the rapid removal of radioactive iodine.
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http://dx.doi.org/10.1002/anie.202010829DOI Listing
December 2020

Dental follicle stem cells rescue the regenerative capacity of inflamed rat dental pulp through a paracrine pathway.

Stem Cell Res Ther 2020 08 3;11(1):333. Epub 2020 Aug 3.

Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, People's Republic of China.

Background: Pulpitis is a common dental disease characterized by sustained inflammation and impaired pulp self-repair. Mesenchymal stem cell-based minimally invasive vital pulp therapy (MSC-miVPT) is a potential treatment method, but its application is limited by the difficulty in acquiring MSCs. We recently revealed the immunomodulatory effects of rat dental follicle stem cells (rDFSCs) on acute lung injury. The present study focused on the paracrine effects of rDFSCs on the inflammation and regeneration of rat injured dental pulp to detect whether DFSCs are a potential candidate for MSC-miVPT.

Methods: Conditioned medium from rDFSCs (rDFSC-CM) was applied to lipopolysaccharide (LPS)-induced inflammatory rat dental pulp cells (rDPCs). The inflammation and regeneration of rDPCs were detected by RT-qPCR, Western blotting, immunofluorescence staining, Cell Counting Kit-8 (CCK-8) assay, flow cytometry, wound-healing assay, and Masson's staining. The effects of rDFSC-CM on inflamed rat dental pulp were further evaluated by hematoxylin-eosin and immunohistochemical staining.

Results: rDFSC-CM downregulated the ERK1/2 and NF-κB signaling pathways, which resulted in suppression of the expression of IL-1β, IL-6, and TNF-α and promotion of the expression of IL-4 and TGF-β, and these findings lead to the attenuation of rDPC inflammation. rDFSC-CM enhanced the in vitro proliferation, migration, and odontogenic differentiation of inflammatory rDPCs and their in vivo ectopic dentinogenesis. Furthermore, rDFSC-CM inhibited inflammatory cell infiltration in rat pulpitis and triggered Runx2 expression in some of the odontoblast-like cells surrounding the injured site, and these effects were conducive to the repair of inflamed dental pulp.

Conclusions: rDFSC-CM exhibits therapeutic potential by rescuing the regeneration of the inflamed rat dental pulp through an immunomodulatory mechanism, indicating the application prospects of DFSCs in biological regenerative endodontics.
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http://dx.doi.org/10.1186/s13287-020-01841-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397608PMC
August 2020

Encodes a MYB Transcription Factor That Regulates Spikelet Development in Rice.

Plant Physiol 2020 09 17;184(1):251-265. Epub 2020 Jul 17.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China.

Rice () spikelets have a unique inflorescence structure, and the mechanisms regulating their development are not yet fully understood. Moreover, approaches to manipulate spikelet development have the potential to increase grain yield. In this study, we identified and characterized a recessive spikelet mutant, namely (). The mutant has a delayed transition from the spikelet to the floral meristem, inducing the formation of extra lemma-like and palea-like organs. In addition, the main body of the palea was reduced, and the sterile lemma was enlarged and partially acquired hull (lemma and/or palea) identity. We used map-based cloning to identify the locus and confirmed our identification by complementation and by generating new alleles using CRISPR-Cas9 gene editing. encodes a MYB domain protein with the typical ethylene response factor-associated amphiphilic repression motifs, is expressed in all organs and tissues, and has a strong repression effect. MOF1 localizes to the nucleus and interacts with TOPLESS-RELATED PROTEINs to possibly repress the expression of downstream target genes. Taken together, our results reveal that plays an important role in the regulation of organ identity and spikelet determinacy in rice.
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http://dx.doi.org/10.1104/pp.20.00658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479877PMC
September 2020

Parent-adolescent congruence in phubbing and adolescents' depressive symptoms: A moderated polynomial regression with response surface analyses.

J Affect Disord 2020 10 30;275:127-135. Epub 2020 Apr 30.

School of Education, Renmin University of China, No. 59 Zhongguancun Street, Haidian District, Beijing 100872, China. Electronic address:

Background: With the widespread of smartphones, there is an increased interest in exploring the influences of phubbing in modern society. However, little research has examined the impact of parent phubbing (Pphubbing) or adolescent phubbing (Aphubbing) on adolescent development. This conceptual and empirical work aims to explore the unique and joint impacts of Pphubbing and Aphubbing on adolescents' depressive symptoms.

Method: Participants were 3322 students from 64 classes of a senior high school. The data were analyzed with polynomial regressions and response surface analyses.

Results: The results showed that Pphubbing was positively related to Aphubbing. Pphubbing and Aphubbing were both positively associated with adolescents' depressive symptoms. In addition, when Pphubbing and Aphubbing were in congruence, adolescents' depressive symptoms would increase as the former two increased. Moreover, adolescents' depressive symptoms would decrease as the discrepancy between parent and adolescent increased. Meanwhile, attachment avoidance moderated the congruence and incongruence effects of parent-adolescent phubbing on adolescents' depressive symptoms.

Limitations: This study used cross-sectional data, which cannot infer causality.

Conclusion: The effect between parent-adolescent congruence in phubbing and adolescents' depressive symptoms is significant, and it is moderated by attachment avoidance.
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http://dx.doi.org/10.1016/j.jad.2020.03.156DOI Listing
October 2020

Epidemiological and clinical characteristics of three family clusters of COVID-19 transmitted by latent patients in China.

Epidemiol Infect 2020 07 6;148:e137. Epub 2020 Jul 6.

Department of Infectious Diseases, Ruian People's Hospital, Ruian, Zhejiang, China.

From 21 January 2020 to 9 February 2020, three family clusters involving 31 patients with coronavirus disease 2019 were identified in Wenzhou, China. The epidemiological and clinical characteristics of the family cluster patients were analysed and compared with those of 43 contemporaneous sporadic cases. The three index cases transmitted the infection to 28 family members 2-10 days before illness onset. Overall, 28 of the 41 sporadic cases and three of 31 patients in the family clusters came back from Wuhan (65.12 vs. 9.68%, P< 0.001). In terms of epidemiological characters and clinical symptoms, no significant differences were observed between the family cluster and sporadic cases. However, the lymphocyte counts of sporadic cases were significantly lower than those of family cluster cases ((1.32 ± 0.55) × 109/l vs. (1.63 ± 0.70) × 109/l, P = 0.037), and the proportion of hypoalbuminaemia was higher in sporadic cases (18/43, 41.86%) than in the family clusters (6/31, 19.35%) (P < 0.05). Within the family cluster, the second- and third-generation cases had milder clinical manifestations, without severe conditions, compared with the index and first-generation cases, indicating that the virulence gradually decreased following passage through generations within the family clusters. Close surveillance, timely recognition and isolation of the suspected or latent patient is crucial in preventing family cluster infection.
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http://dx.doi.org/10.1017/S0950268820001491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360955PMC
July 2020

Retrospective detection of SARS-CoV-2 in hospitalized patients with influenza-like illness.

Emerg Microbes Infect 2020 Dec;9(1):1470-1473

Research Laboratory of Clinical Virology, Ruijin Hospital, and Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Since the first report of the coronavirus disease (COVID-19) in late December 2019, the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has now widely spread to more than 187 countries and regions. However, it is unclear whether there has been cryptic transmission before these early officially confirmed cases, we therefore retrospectively screened for the SARS-CoV-2 RNA in 1271 nasopharyngeal swab samples, as well as the prevalence of IgM, IgG, and total antibodies against SARS-CoV-2 in 357 matched serum samples collected from hospitalized patients with influenza-like illness between 1 December 2018 and 31 March 2020 in Shanghai Ruijin Hospital. The onset date of the earliest COVID-19 case in this study was 25 January 2020. Before this time point, the presence of SARS-CoV-2 was not observed, which limited the possibility that SARS-CoV-2 has already spread among the population before the large-scale outbreak. Additionally, among 6662 patients with influenza-like illness from 1 December 2017 to 31 March 2020, the overall number of patients positive for influenza and other respiratory viruses during the COVID-19 period decreased significantly when compared with that in the same period of the last two years, reflecting that public health interventions can effectively control the spread of common respiratory viruses.
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http://dx.doi.org/10.1080/22221751.2020.1785952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473166PMC
December 2020

Work Stress and General Trust: The Mediating Effect of Depression and the Moderating Effect of the OXTR Gene rs53576.

J Affect Disord 2020 07 29;272:283-288. Epub 2020 Apr 29.

School of Psychological and Cognitive Sciences and Beijing Key Laboratory of Behavior and Mental Health, Peking University, 5# Yiheyuan Road, Haidian District, Beijing, 100871. Electronic address:

Background: Work stress has been proved to be linked to depression, and both can decrease general trust. Likewise, OXTR gene is currently the gene that is most closely associated with trust. However, few empirical studies have explored the significant role of gene-environment interactions on general trust. In this study, we explored the mediating role of depression in the relationship between work stress and general trust, and the moderating role of the OXTR rs 53576 in the mediation model.

Methods: 364 healthy Han Chinese faculties were recruited from one university in Beijing. Work stress, depression, and general trust were assessed using the House and Rizzo's Work Stress Scale, Self-Rating Depression Scale (SDS), and the General Trust Questionnaire, respectively. Blood samples were collected for OXTR rs53576 genotyping.

Results: Depression mediated the relationship between work stress and general trust. In the mediation model, OXTR rs53576 could only moderated the direct path from work stress to general trust. For AA and GA individuals, depression completely mediated the relationship between work stress and general trust. For GG individuals, depression partly mediated the relationship between work stress and general trust.

Limitations: This study used a cross-sectional design, only considered Han Chinese faculties, and the role of other genes should be explored.

Conclusions: Depression has a mediating effect between work stress and general trust. The general trust levels of GG genotype individuals are more prone to be affected by work stress than AA and GA individuals.
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http://dx.doi.org/10.1016/j.jad.2020.03.160DOI Listing
July 2020

A near-IR Fluorescent Probe for Enantioselective Recognition of Amino Acids in Aqueous Solution.

J Org Chem 2020 06 27;85(11):7342-7348. Epub 2020 May 27.

Department of Chemistry, University of Virginia, McCormick Rd, Charlottesville, Virginia 22904, United States.

A novel BINOL-based near-IR fluorescent probe was designed and synthesized by incorporating a rhodamine-like dye. In the presence of Zn(II), this compound was found to exhibit highly enantioselective fluorescence enhancement at λ > 730 nm and λ = 690 nm when treated with 14 common amino acids in aqueous solution. An enantioselective fluorescence enhancement ratio up to 163 was observed. The mechanism of the fluorescence response of this probe was investigated by various spectroscopic methods.
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http://dx.doi.org/10.1021/acs.joc.0c00725DOI Listing
June 2020

Effectiveness of convalescent plasma therapy in severe COVID-19 patients.

Proc Natl Acad Sci U S A 2020 04 6;117(17):9490-9496. Epub 2020 Apr 6.

Sinopharm Wuhan Plasma-derived Biotherapies Co., Ltd, 430207 Wuhan, China.

Currently, there are no approved specific antiviral agents for novel coronavirus disease 2019 (COVID-19). In this study, 10 severe patients confirmed by real-time viral RNA test were enrolled prospectively. One dose of 200 mL of convalescent plasma (CP) derived from recently recovered donors with the neutralizing antibody titers above 1:640 was transfused to the patients as an addition to maximal supportive care and antiviral agents. The primary endpoint was the safety of CP transfusion. The second endpoints were the improvement of clinical symptoms and laboratory parameters within 3 d after CP transfusion. The median time from onset of illness to CP transfusion was 16.5 d. After CP transfusion, the level of neutralizing antibody increased rapidly up to 1:640 in five cases, while that of the other four cases maintained at a high level (1:640). The clinical symptoms were significantly improved along with increase of oxyhemoglobin saturation within 3 d. Several parameters tended to improve as compared to pretransfusion, including increased lymphocyte counts (0.65 × 10/L vs. 0.76 × 10/L) and decreased C-reactive protein (55.98 mg/L vs. 18.13 mg/L). Radiological examinations showed varying degrees of absorption of lung lesions within 7 d. The viral load was undetectable after transfusion in seven patients who had previous viremia. No severe adverse effects were observed. This study showed CP therapy was well tolerated and could potentially improve the clinical outcomes through neutralizing viremia in severe COVID-19 cases. The optimal dose and time point, as well as the clinical benefit of CP therapy, needs further investigation in larger well-controlled trials.
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http://dx.doi.org/10.1073/pnas.2004168117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7196837PMC
April 2020

Opposite Enantioselectivity of Mg(II) Versus Zn(II) in the Fluorescent Recognition of Amino Acids.

J Org Chem 2020 04 25;85(7):4901-4905. Epub 2020 Mar 25.

Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904-4319, United States.

The addition of Mg is found to turn on the fluorescence response of a molecular probe, 3,3'-diformyl-1,1'-bi-2-naphthol, toward chiral amino acids with high enantioselectivity. It is further found that the enantioselective fluorescence responses of the molecular probe in the presence of Mg toward certain amino acids are the opposite of those in the presence of Zn, that is, using Mg with an l-amino acid generates much greater fluorescence enhancement than with the corresponding d-amino acid, but using Zn with the d-amino acid gives much greater fluorescence than with the l-enantiomer. Thus, simply changing the metal cation additive allows the chirality sense of the fluorescence-based molecular recognition to be easily regulated.
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http://dx.doi.org/10.1021/acs.joc.0c00064DOI Listing
April 2020

Sulfonation of 3,3'-Diformyl-BINOL for Enantioselective Fluorescent Recognition of Amino Acids in Water.

Chemistry 2020 Jun 11;26(32):7258-7262. Epub 2020 May 11.

Department of Chemistry, University of Virginia, McCormick Rd, Charlottesville, VA, 22904, USA.

Reaction of (R)-3,3'-diformyl-1,1'-bi-2-naphthol with concentrated sulfuric acid gives the corresponding 6,6'-disulfonated compound (R)-2 selectively. This provides a simple and efficient method to convert a water-insoluble compound to a water-soluble fluorescent probe. It is found that (R)-2 in combination with Zn shows a highly enantioselective fluorescent response toward various amino acids in the aqueous HEPES buffer solution at pH 7.4. For example, an enantioselective fluorescence enhancement ratio [ef=ΔI /ΔI ] up to 35.8 is observed for the recognition of asparagine. NMR and mass spectroscopic investigations are conducted to explore the reaction of (R)-2 with asparagine.
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http://dx.doi.org/10.1002/chem.202000423DOI Listing
June 2020

Macrophage-derived sulfur dioxide is a novel inflammation regulator.

Biochem Biophys Res Commun 2020 04 10;524(4):916-922. Epub 2020 Feb 10.

Department of Pediatrics, Peking University First Hospital, Beijing, China. Electronic address:

Macrophage-mediated inflammation is a key pathophysiological component of cardiovascular diseases, but the underlying mechanisms by which the macrophage regulates inflammation have been unclear. In our study, we, for the first time, showed an endogenous sulfur dioxide (SO) production in RAW267.4 macrophages by using HPLC and SO-specific fluorescent probe assays. Moreover, the endogenous SO generating enzyme aspartate aminotransferase (AAT) was found to be expressed by the macrophages. Furthermore, we showed that AAT2 knockdown triggered spontaneous macrophage-mediated inflammation, as represented by the increased TNF-α and IL-6 levels and the enhanced macrophage chemotaxis; these effects could be reversed by the treatment with a SO donor. Mechanistically, AAT2 knockdown activated the NF-κB signaling pathway in macrophages, while SO successfully rescued NF-κB activation. In contrast, forced AAT2 expression reversed AngII-induced NF-κB activation and subsequent macrophage inflammation. Moreover, treatment with a SO donor also alleviated macrophage infiltration in AngII-treated mouse hearts. Collectively, our data suggest that macrophage-derived SO is an important regulator of macrophage activation and it acts as an endogenous "on-off switch" in the control of macrophage activation. This knowledge might enable a new therapeutic strategy for cardiovascular diseases.
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http://dx.doi.org/10.1016/j.bbrc.2020.02.013DOI Listing
April 2020

ABNORMAL FLOWER AND GRAIN 1 encodes OsMADS6 and determines palea identity and affects rice grain yield and quality.

Sci China Life Sci 2020 Feb 8;63(2):228-238. Epub 2020 Jan 8.

State Key Laboratory of Rice Biology, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, 310006, China.

The palea and lemma are floral organ structures unique to grasses; these structures form the hull and directly affect grain size and quality. However, the molecular mechanisms controlling the development of the hull are not well understood. In this study, we characterized the rice (Oryza sativa) abnormal flower and grain1 (afg1) mutant, a new allele of OsMADS6. Similar to previously characterized osmads6 alleles, in the afg1 floret, the palea lost its marginal region and acquired the lemma identity. However, in contrast to other osmads6 alleles, the afg1 mutant showed altered grain size and grain quality, with decreased total starch and amylose contents, and increased protein and soluble sugar contents. The analysis of transcriptional activity suggested that AFG1 is a transcriptional activator and may affect grain size by regulating the expression levels of several genes related to cell expansion and proliferation in the afg1 mutant. These results revealed that AFG1 plays an important role in determining palea identity and affecting grain yield and quality in rice.
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http://dx.doi.org/10.1007/s11427-019-1593-0DOI Listing
February 2020

AH2 encodes a MYB domain protein that determines hull fate and affects grain yield and quality in rice.

Plant J 2019 11 3;100(4):813-824. Epub 2019 Sep 3.

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, People's Republic of China.

The palea and lemma (hull) are grass-specific organs, and determine grain size and quality. In the study, AH2 encodes a MYB domain protein, and functions in the development of hull and grain. Mutation of AH2 produces smaller grains and alters grain quality including decreased amylose content and gel consistency, and increased protein content. Meantime, part of the hull lost the outer silicified cells, and induces a transformation of the outer rough epidermis to inner smooth epidermis cells, and the body of the palea was reduced in the ah2 mutant. We confirmed the function of AH2 by complementation, CRISPR-Cas9, and cytological and molecular tests. Additionally, AH2, as a repressor, repress transcription of the downstream genes. Our results revealed that AH2 plays an important role in the determination of hull epidermis development, palea identity, and grain size.
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http://dx.doi.org/10.1111/tpj.14481DOI Listing
November 2019

Simultaneous Determination of Concentration and Enantiomeric Composition of Amino Acids in Aqueous Solution by Using a Tetrabromobinaphthyl Dialdehyde Probe.

Chemistry 2019 Jul 3;25(42):9967-9972. Epub 2019 Jul 3.

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, 610064, P.R. China.

3,3'-Diformyl-1,1'-bi-2-naphthol or its methoxymethyl-protected derivative is found to undergo a highly selective reaction with excess bromine in CH Cl at reflux to give the novel 5,5',6,6'-tetrabrominated product (S)- or (R)-2. The observed electrophilic substitution at the 5,5'-positons of an optically active binaphthyl compound is unprecedented. Unlike unbrominated 3,3'-diformyl-1,1'-bi-2-naphthol, which is not suitable for fluorescent recognition in water, compound (S)-2, in combination with Zn , exhibits a highly enantioselective fluorescent response toward amino acids in aqueous solution (HEPES buffer, pH 7.4). It is further found that the condensation product of (R)-2 with tryptophan, (R)-3, shows dual-responsive emissions toward amino acids; the short wavelength (λ =350 nm) emission is sensitive to the concentration of the substrate regardless of the chiral configuration and the long wavelength (λ >500 nm) emission is highly enantioselective. Thus, the use of (R)-3 allows the simultaneous determination of the concentration and enantiomeric composition of an amino acid sample from one fluorescence measurement.
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http://dx.doi.org/10.1002/chem.201901374DOI Listing
July 2019

Dental Follicle Stem Cells Ameliorate Lipopolysaccharide-Induced Inflammation by Secreting TGF-β3 and TSP-1 to Elicit Macrophage M2 Polarization.

Cell Physiol Biochem 2018 7;51(5):2290-2308. Epub 2018 Dec 7.

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou,

Background/aims: Increasing evidence has demonstrated the novel roles of mesenchymal stem cells (MSCs) in immunotherapy. However, difficulty in acquiring these cells and possible ethical issues limited their application. Recently, we have isolated a unique MSC population from dental follicles with potent stem cell-like properties. This study focused on the effects of dental follicle stem cells (DFSCs) on macrophage activation and polarization to determine their role in immunomodulation and to test if DFSCs are a promising cell source for MSC-based immunotherapy.

Methods: Rat acute lung injury (ALI) models induced by Lipopolysaccharide (LPS) were applied to test the immune-modulatory effects of DFSC/DFSC-CM in vivo. The pulmonary permeability was determined by the dry / wet weight ratios of the left upper lung lobe. The lung histopathological damage was graded on a 0 to 4+ scale. And the inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were tested by ELISA. Then we established LPS-induced inflamed macrophage models in vitro. Inflammatory cytokine production and polarization marker expression were measured by RT-qPCR, ELISA, western blot and flow cytometric analysis in macrophages following DFSC-CM treatment. The paracrine factors in DFSC-CM were revealed by a RayBiotech Protein Array. Thereafter, neutralization studies were performed to confirm the potential immune regulators in DFSC-CM.

Results: The DFSC/DFSC-CM not only attenuated histopathological damage and pulmonary permeability, but also downregulated pro-inflammatory cytokines MCP-1, IL-1, IL-6 and TNF-α, and upregulated anti-inflammatory cytokine IL-10 in BALF. Immunofluorescence staining revealed the increased expression of macrophage M2 marker Arg-1. Further in vitro study revealed that macrophages switched to an anti-inflammatory M2 phenotype when co-cultured with DFSC-CM, characterized by suppressed production of pro-inflammatory cytokines MCP-1, IL-1, IL-6, TNF-α and M1-polarizing markers iNOS and CD86; and increased expression of the anti-inflammatory cytokine IL-10 and the M2-polarizing markers Arg-1 and CD163. A RayBiotech Protein Array revealed 42 differentially expressed (> 2-fold) paracrine factors in DFSC-CM compared with the serum-free Ham's F-12K medium, among which TGF-β3 and Thrombospondin-1 (TSP-1) were upregulated by 18- and 105-fold, respectively. Neutralization studies confirmed the immunoregulatory roles of TGF-β3 and TSP-1 in macrophage activation and polarization.

Conclusion: These results indicated that DFSCs can reprogram macrophages into the anti-inflammatory M2 phenotype, the paracrine factors TGF-β3 and TSP-1 may be one of the underlying mechanisms. This study supports the hypothesis that DFSCs are promising for MSC-based immunotherapy.
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http://dx.doi.org/10.1159/000495873DOI Listing
January 2019
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