Publications by authors named "Xiaopeng Liu"

92 Publications

Downregulation of FPN1 acts as a prognostic biomarker associated with immune infiltration in lung cancer.

Aging (Albany NY) 2021 Mar 10;13(6):8737-8761. Epub 2021 Mar 10.

Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China.

Lung cancer morbidity and mortality remain the leading causes of tumor-associated death worldwide. The discovery of early diagnostic and prognostic markers of lung cancer could significantly improve the survival rate and decrease the mortality rate. FPN1 is the only known mammalian iron exporter. However, the molecular and biological functions of FPN1 in lung cancer remain unclear. Here, FPN1 mRNA expression in lung cancer was estimated using the TCGA, Oncomine, TIMER, and UALCAN databases. The prognostic role of FPN1 was evaluated using Kaplan-Meier plotter and PrognoScan. Associations between FPN1 and immune infiltration in lung cancer were evaluated by the TIMER and CIBERSORT algorithms. FPN1 mRNA and protein expressions were significantly downregulated in lung cancer. Low FPN1 expression was strongly related to worse prognosis in patients with lung cancer. GO and KEGG analyses and GSEA suggested that FPN1 was remarkably related to iron homeostasis and immunity. Importantly, FPN1 was remarkably associated with the infiltrating abundance of multiple immune cells. Moreover, FPN1 displayed a strong correlation with various immune marker sets. We investigated the clinical application value of FPN1 and provided a basis for the sensitive diagnosis, prognostication and targeted therapy of lung cancer.
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http://dx.doi.org/10.18632/aging.202685DOI Listing
March 2021

Temporal characteristics of the cochlear response after noise exposure.

Hear Res 2021 May 18;404:108208. Epub 2021 Feb 18.

Center for Hearing and Deafness, SUNY at Buffalo, Buffalo, New York 14214, USA.

The effect of intense noise on cochlear sensitivity has been extensively studied, but its influence on the temporal characteristics of the cochlear response is still unclear. This study investigated the effects of noise exposure on the latency of cochlear response and cochlear forward masking. Rats were exposed to an octave band noise (8-16 kHz) at 90 dB SPL for 5 days. Cochlear compound action potentials (CAPs) induced by single- and double-tone stimuli and distortion product otoacoustic emissions (DPOAE) were recorded 1 day or 2 months after the noise exposure. The latency of the CAP and its forward masking were compared between the noise-exposed rats and normal control rats. The noise exposure significantly reduced DPOAE and elevated CAP threshold in the noise band region, but not in the other areas. Even in the noise band area, the noise did not reduce CAP-amplitude at the high stimulation level (80 dB SPL). Correspondingly, about one-third of the outer hair cells (OHC) in the noise band area disappeared, while the inner hair cells (IHC) did not. However, the noise exposure in the frequency range of 4-24 kHz significantly prolonged CAP latency and increased its variability, while the CAP forward masking effect was significantly enhanced in the frequency range of 16-30 kHz. The frequency-dependent changes in CAP latency and forward masking after noise exposure may reflect different types of synaptic subinjury in the cochlea, which may lead to psychophysical consequences of sound localization and speech recognition.
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http://dx.doi.org/10.1016/j.heares.2021.108208DOI Listing
May 2021

Neuroplastic changes in auditory cortex induced by long-duration "non-traumatic" noise exposures are triggered by deficits in the neural output of the cochlea.

Hear Res 2021 May 12;404:108203. Epub 2021 Feb 12.

Center for Hearing and Deafness, SUNY at Buffalo, Buffalo, 137 Cary Hall, 3435 Main Street, NY 14214, USA.

Long-term exposure to moderate intensity noise that does not cause measureable hearing loss can cause striking changes in sound-evoked neural activity in auditory cortex.  It is unclear if these changes originate in the cortex or result from functional deficits in the neural output of the cochlea.  To explore this issue, rats were exposed for 6-weeks to 18-24 kHz noise at 45, 65 or 85 dB SPL and then compared the noise-induced changes in the cochlear compound action potential (CAP) with the neurophysiological alterations in the anterior auditory field (AAF) of auditory cortex. The 45-dB exposure, which had no effect on the cochlear CAP also had no effect on the AAF. In contrast, the 85-dB exposure greatly reduced CAP amplitudes at high frequencies, but had little or no effect on low frequencies. Despite the large reduction in high-frequency CAP neural responses, high frequency AAF neural responses (spike rate and local field potential amplitude) remained largely within normal limits, evidence of central gain compensation. AAF responses were also enhanced at the low frequencies even though CAP responses were normal; this AAF hyperactivity only occurred at low-moderate intensities (level-dependent enhanced central gain). The 65-dB exposure also caused a moderate reduction in high-frequency CAP amplitudes. Notwithstanding this cochlear loss, AAF responses were boosted into the normal range, evidence of homeostatic gain compensation. Our results suggest that the noise-induced neuroplastic changes in the auditory cortex from so-called "non-traumatic" exposures are triggered from functional deficits in the neural output of the cochlea.
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http://dx.doi.org/10.1016/j.heares.2021.108203DOI Listing
May 2021

Primary enteric adenocarcinoma: A case report and literature review.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2020 Dec;45(12):1504-1508

Department of Respiratory and Critical Care Medicine, Second Xiangya Hospital, Changsha 410011.

Primary enteric adenocarcinoma is a rare variant of primary pulmonary adenocarcinoma. This disease lacks a distinctive manifestation and often requires pathological examination to make a definite diagnosis. A male patient visited the Second Xiangya Hospital, Central South University for consistent cough and sputum production for about 1 year. Anti-infection therapy was given but it showed ineffectiveness. Enteric adenocarcinoma was diagnosed after percutaneous lung biopsy according to pathological findings. Combining this case with relevant literature, we summarized the characteristics to raise physicians' awareness for this rare subtype.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2020.190392DOI Listing
December 2020

Review: Neural Mechanisms of Tinnitus and Hyperacusis in Acute Drug-Induced Ototoxicity.

Am J Audiol 2021 Jan 19:1-15. Epub 2021 Jan 19.

Center for Hearing & Deafness, Department of Communicative Disorders and Sciences, The State University of New York at Buffalo.

Purpose Tinnitus and hyperacusis are debilitating conditions often associated with age-, noise-, and drug-induced hearing loss. Because of their subjective nature, the neural mechanisms that give rise to tinnitus and hyperacusis are poorly understood. Over the past few decades, considerable progress has been made in deciphering the biological bases for these disorders using animal models. Method Important advances in understanding the biological bases of tinnitus and hyperacusis have come from studies in which tinnitus and hyperacusis are consistently induced with a high dose of salicylate, the active ingredient in aspirin. Results Salicylate induced a transient hearing loss characterized by a reduction in otoacoustic emissions, a moderate cochlear threshold shift, and a large reduction in the neural output of the cochlea. As the weak cochlear neural signals were relayed up the auditory pathway, they were progressively amplified so that the suprathreshold neural responses in the auditory cortex were much larger than normal. Excessive central gain (neural amplification), presumably resulting from diminished inhibition, is believed to contribute to hyperacusis and tinnitus. Salicylate also increased corticosterone stress hormone levels. Functional imaging studies indicated that salicylate increased spontaneous activity and enhanced functional connectivity between structures in the central auditory pathway and regions of the brain associated with arousal (reticular formation), emotion (amygdala), memory/spatial navigation (hippocampus), motor planning (cerebellum), and motor control (caudate/putamen). Conclusion These results suggest that tinnitus and hyperacusis arise from aberrant neural signaling in a complex neural network that includes both auditory and nonauditory structures.
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http://dx.doi.org/10.1044/2020_AJA-20-00023DOI Listing
January 2021

LncRNA miR143HG inhibits the proliferation of glioblastoma cells by sponging miR-504.

Int J Neurosci 2021 Feb 2:1-9. Epub 2021 Feb 2.

Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang City, China.

Aim: It is known that miR-504 can target p53 to promote cancer progression. Our bioinformatics analysis revealed that miR-504 could bind miR-143 host gene (miR143HG), suggesting that miR143HG might also have crosstalk with p53 in cancer progression. This study aimed to analyze the function of miR143HG in glioblastoma (GBM).

Methods: This study selected 64 GBM patients. GBM and non-tumor tissues were obtained from the patients. RT-qPCR was used to analyze gene expression. Survival curve analysis was performed to analyze the prognostic values of miR143HG for GBM. The crosstalk between miR143HG and miR-504 was analyzed by overexpressing them in GBM cells, followed by RT-qPCRs to detect their expression. CCK-8 assay was used to detect the cell proliferation ability.

Results: We found that miR143HG was downregulated in GBM and predicted poor survival. The mRNA expression levels of miR143HG and p53 were positively correlated in GBM tissues. Bioinformatics analysis suggested that miR143HG could form base paring with miR-504, which has been reported to target p53. Overexpression experiments revealed that miR143HG overexpression upregulated the expression of p53, while miR-504 overexpression inhibited the effect of miR143HG overexpression on the expression of p53. Moreover, overexpression of miR143HG and p53 decreased GBM cell proliferation, while overexpression of miR-504 increased GBM cell proliferation. In addition, overexpression of miR-504 attenuated the effect of miR143HG overexpression on GBM cell proliferation.

Conclusion: Therefore, miR143HG may decrease the proliferation of GBM cells by sponging miR-504 to upregulate p53.
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http://dx.doi.org/10.1080/00207454.2020.1865950DOI Listing
February 2021

Nuciferine protects against folic acid-induced acute kidney injury by inhibiting ferroptosis.

Br J Pharmacol 2021 Mar;178(5):1182-1199

Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, China.

Background And Purpose: Acute kidney injury is a common clinical problem with no definitive or specific treatment. Therefore, the molecular mechanisms of acute kidney injury must be fully understood to develop novel treatments. Nuciferine, a major bioactive compound isolated from the lotus leaf, possesses extensive pharmacological activities. Its effect on folic acid-induced acute kidney injury, however, remains unknown. Here, we aimed to clarify the pharmacological effects of nuciferine and its mechanisms of action in acute kidney injury.

Experimental Approach: The effects of nuciferine on folic acid-induced acute kidney injury in mice were investigated. HK-2 human proximal tubular epithelial cells and HEK293T HEK cells were used to evaluate the protective effect of nuciferine on RSL3-induced ferroptosis.

Key Results: Nuciferine treatment mitigated the pathological alterations, ameliorated inflammatory cell infiltration and improved kidney dysfunction in mice with folic acid-induced acute kidney injury. In HK-2 and HEK293T cells, nuciferine significantly prevented RSL3-induced ferroptotic cell death. Mechanistically, nuciferine significantly inhibited ferroptosis by preventing iron accumulation and lipid peroxidation in vitro and in vivo. Moreover, knockdown of glutathione (GSH) peroxidase 4 (GPX4) abolished the protective effect of nuciferine against ferroptosis.

Conclusion And Implications: Nuciferine ameliorated renal injury in mice with acute kidney injury, perhaps by inhibiting the ferroptosis. Nuciferine may represent a novel treatment that improves recovery from acute kidney injury by targeting ferroptosis.
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http://dx.doi.org/10.1111/bph.15364DOI Listing
March 2021

A novel identified Pseudomonas aeruginosa, which exhibited nitrate- and nitrite-dependent methane oxidation abilities, could alleviate the disadvantages caused by nitrate supplementation in rumen fluid fermentation.

Microb Biotechnol 2020 Dec 11. Epub 2020 Dec 11.

College of Animal Science and Technology, Northwest A&F University, Shaanxi, 712100, China.

After the occurrence of nitrate-dependent anaerobic methane oxidation (AMO) in rumen fluid culture was proved, the organisms that perform the denitrifying anaerobic methane oxidizing (DAMO) process in the rumen of dairy goat were investigated by establishing two enrichment culture systems, which were supplied with methane as the sole carbon source and NaNO or NaNO as the electron acceptor. Several Operational Taxonomic Units (OTU) belonging to Proteobacteria became dominant in the two enrichment systems. The identified Pseudomonas aeruginosa, which was isolated from the NaNO enrichment system, could individually perform a whole denitrifying anaerobic methane oxidizing process. Further in vitro rumen fermentation showed that supplementation with the isolated P. aeruginosa could reduce methane emissions, alleviate the nitrite accumulation and prevent the decrease in propionic acid product caused by nitrate supplementation.
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http://dx.doi.org/10.1111/1751-7915.13726DOI Listing
December 2020

Psychophysiological Responses of Pilots in Hypoxia Training at 7000 and 7500 m.

Aerosp Med Hum Perform 2020 Oct;91(10):785-789

We compared the physiological responses, psychomotor performances, and hypoxia symptoms between 7000 m and 7500 m (23,000 and 24,600 ft) exposure to develop a safer hypoxia training protocol. In altitude chamber, 66 male pilots were exposed to 7000 and 7500 m. Heart rate and arterial oxygen saturation were continuously monitored. Psychomotor performance was assessed using the computational task. The hypoxic symptoms were investigated by a questionnaire. The mean duration time of hypoxia was 323.0 56.5 s at 7000 m and 218.2 63.3 s at 7500 m. The 6-min hypoxia training was completed by 57.6% of the pilots and 6.1% of the pilots at 7000 m and at 7500 m, respectively. There were no significant differences in pilots heart rates and psychomotor performance between the two exposures. The So₂ response at 7500 m was slightly severer than that at 7000 m. During the 7000 m exposure, pilots experienced almost the same symptoms and similar frequency order as those during the 7500 m exposure. There were concordant symptoms, psychomotor performance, and very similar physiological responses between 7000 m and 7500 m during hypoxia training. The results indicated that 7000-m hypoxia awareness training might be an alternative to 7500-m hypoxia training with lower DCS risk and longer experience time.
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http://dx.doi.org/10.3357/AMHP.5634.2020DOI Listing
October 2020

Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae.

Biomolecules 2020 10 16;10(10). Epub 2020 Oct 16.

Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Biomedical Sciences, University of Science and Technology of China, Hefei 230027, China.

Cochlear hair cells in human beings cannot regenerate after loss; however, those in fish and other lower species can. Recently, the role of inflammation in hair cell regeneration has been attracting the attention of scientists. In the present study, we investigated how suppression of inflammatory factors affects hair cell regeneration and the functional recovery of regenerated hair cells in zebrafish. We killed hair cells in the lateral line of zebrafish larvae with CuSO to induce an inflammatory response and coapplied BRS-28, an anti-inflammatory agent to suppress the inflammation. The recovery of the hair cell number and rheotaxis was slower when CuSO and BRS-28 were coapplied than when CuSO was applied alone. The recovery of hair cell count lagged behind that of the calcium imaging signal during the regeneration. The calcium imaging signal in the neuromasts in the inflammation-inhibited group was weaker than that in the noninflammation-inhibited group at the early stage of regeneration, although it returned to normal at the late stage. Our study demonstrates that suppressing inflammation by BRS-28 delays hair cell regeneration and functional recovery when hair cells are damaged. We suspect that BRS-28 inhibits pro-inflammatory factors and thereby reduces the migration of macrophages to delay the regeneration of hair cells.
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http://dx.doi.org/10.3390/biom10101451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650643PMC
October 2020

Surgical treatment of primary pulmonary artery sarcoma.

Gen Thorac Cardiovasc Surg 2021 Apr 12;69(4):638-645. Epub 2020 Sep 12.

Peking University China-Japan Friendship School of Clinical Medicine, Beijing, 100029, China.

Objective: Primary pulmonary artery sarcoma (PAS) is a rare tumor that originates from the intimal layer of the pulmonary artery or pulmonary valve and has a poor prognosis. The standard treatment for this devastating disease remains unclear. This study aimed to summarize the current standard treatments for PAS.

Methods: From September 2015 to January 2020, six patients were diagnosed with PAS and underwent pulmonary endarterectomy (PEA) at our department. Their medical records were retrospectively reviewed to analyze the clinical characteristics, histopathological features, and postoperative outcomes. Fourteen articles, each reporting at least 6 cases, identified 201 patients diagnosed with PAS, and 158 patients had detailed treatments and follow-up data.

Results: All of the patients who successfully underwent PEA were alive at follow-up, with a mean survival duration of 11.6 months (7-28 months), and one patient developed recurrence in the right upper lobe lung. Two patients received postoperative chemotherapy. In one patient, the tumor invaded the pulmonary valve.

Conclusions: PAS resection combined with PEA via the aid of cardiopulmonary bypass and deep hypothermic circulatory arrest could achieve maximal tumor resection in patients without metastatic lesions. An individualized surgery strategy relies on a precise preoperative imaging examination. Moreover, postoperative adjuvant therapy could yield improved survival outcomes.
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http://dx.doi.org/10.1007/s11748-020-01476-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981312PMC
April 2021

Defect-Enhanced CO Reduction Catalytic Performance in O-Terminated MXenes.

ChemSusChem 2020 Nov 9;13(21):5690-5698. Epub 2020 Sep 9.

School of Materials Science and Engineering, Beihang University, Beijing, 100191, P. R. China.

Electrochemical carbon dioxide reduction reaction (CO RR) represents a promising way to generate fuels and chemical feedstock sustainably. Recently, studies have shown that two-dimensional metal carbides and nitrides (MXenes) can be promising CO RR electrocatalysts due to the alternating -C and -H coordination with intermediates that decouples scaling relations seen on transition metal catalysts. However, further by tuning the electronic and surface structure of MXenes it should still be possible to reach higher turnover number and selectivities. To this end, defect engineering of MXenes for electrochemical CO RR has not been investigated to date. In this work, first-principles modelling simulations are employed to systematically investigate CO RR on M XO -type MXenes with transition metal and carbon/nitrogen vacancies. We found that the -C-coordinated intermediates take the form of fragments (e. g., *COOH, *CHO) whereas the -H-coordinated intermediates form a complete molecule (e. g., *HCOOH, *H CO). Interestingly, the fragment-type intermediates become more strongly bound when transition-metal vacancies are present on most MXenes, while the molecule-type intermediates are largely unaffected, allowing the CO RR overpotential to be tuned. The most promising defective MXene is Hf NO containing Hf vacancies, with a low overpotential of 0.45 V. More importantly, through electronic structure analysis it could be observed that the Fermi level of the MXene changes significantly in the presence of vacancies, indicating that the Fermi level shift can be used as an ideal descriptor to rapidly predict the catalytic performance of defective MXenes. Such an evaluation strategy is applicable to other catalysts beyond MXenes, which could enhance high throughput screening efforts for accelerated catalyst discovery.
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http://dx.doi.org/10.1002/cssc.202001624DOI Listing
November 2020

Functional Neuroanatomy of Salicylate- and Noise-Induced Tinnitus and Hyperacusis.

Curr Top Behav Neurosci 2020 Jul 12. Epub 2020 Jul 12.

Center for Hearing and Deafness, University at Buffalo, Buffalo, NY, USA.

Tinnitus and hyperacusis are debilitating conditions often associated with aging or exposure to intense noise or ototoxic drugs. One of the most reliable methods of inducing tinnitus is with high doses of sodium salicylate, the active ingredient in aspirin. High doses of salicylate have been widely used to investigate the functional neuroanatomy of tinnitus and hyperacusis. High doses of salicylate have been used to develop novel behavioral methods to detect the presence of tinnitus and hyperacusis in animal models. Salicylate typically induces a hearing loss of approximately 20 dB which greatly reduces the neural output of the cochlea. As this weak neural signal emerging from the cochlea is sequentially relayed to the cochlear nucleus, inferior colliculus, medial geniculate, and auditory cortex, the neural response to suprathreshold sounds is progressively amplified by a factor of 2-3 by the time the signal reaches the auditory cortex, a phenomenon referred to as enhanced central gain. Sound-evoked hyperactivity also occurred in the amygdala, a region that assigns emotional significance to sensory stimuli. Resting state functional magnetic imaging of the BOLD signal revealed salicylate-induced increases in spontaneous neural activity in the inferior colliculus, medial geniculate body, and auditory cortex as well as in non-auditory areas such as the amygdala, reticular formation, cerebellum, and other sensory areas. Functional connectivity of the BOLD signal revealed increased neural coupling between several auditory areas and non-auditory areas such as the amygdala, cerebellum, reticular formation, hippocampus, and caudate/putamen; these strengthened connections likely contribute to the multifaceted dimensions of tinnitus. Taken together, these results suggest that salicylate-induced tinnitus disrupts a complex neural network involving many auditory centers as well as brain regions involved with emotion, arousal, memory, and motor planning. These extra-auditory centers embellish the basic auditory percepts that results in tinnitus and which may also contribute to hyperacusis.
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http://dx.doi.org/10.1007/7854_2020_156DOI Listing
July 2020

2-Hydroxypropyl-β-cyclodextrin Ototoxicity in Adult Rats: Rapid Onset and Massive Destruction of Both Inner and Outer Hair Cells Above a Critical Dose.

Neurotox Res 2020 Oct 30;38(3):808-823. Epub 2020 Jun 30.

Center for Hearing and Deafness, 137 Cary Hall, University at Buffalo, Buffalo, NY, 14214, USA.

2-Hydroxypropyl-β-cyclodextrin (HPβCD), a cholesterol chelator, is being used to treat diseases associated with abnormal cholesterol metabolism such as Niemann-Pick C1 (NPC1). However, the high doses of HPβCD needed to slow disease progression may cause hearing loss. Previous studies in mice have suggested that HPβCD ototoxicity results from selective outer hair cell (OHC) damage. However, it is unclear if HPβCD causes the same type of damage or is more or less toxic to other species such as rats, which are widely used in toxicity research. To address these issues, rats were given a subcutaneous injection of HPβCD between 500 and 4000 mg/kg. Distortion product otoacoustic emissions (DPOAE), the cochlear summating potential (SP), and compound action potential (CAP) were used to assess cochlear function followed by quantitative analysis of OHC and inner hair cell (IHC) loss. The 3000- and 4000-mg/kg doses abolished DPOAE and greatly reduced SP and CAP amplitudes. These functional deficits were associated with nearly complete loss of OHC as well as ~ 80% IHC loss over the basal two thirds of the cochlea. The 2000-mg/kg dose abolished DPOAE and significantly reduced SP and CAP amplitudes at the high frequencies. These deficits were linked to OHC and IHC losses in the high-frequency region of the cochlea. Little or no damage occurred with 500 or 1000 mg/kg of HPβCD. The HPβCD-induced functional and structural deficits in rats occurred suddenly, involved damage to both IHC and OHC, and were more severe than those reported in mice.
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http://dx.doi.org/10.1007/s12640-020-00252-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484207PMC
October 2020

FOXO3a-mediated long non-coding RNA LINC00261 resists cardiomyocyte hypoxia/reoxygenation injury via targeting miR23b-3p/NRF2 axis.

J Cell Mol Med 2020 08 18;24(15):8368-8378. Epub 2020 Jun 18.

Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, HeBei, China.

Ischemia/reperfusion (I/R)-mediated acute myocardial infarction (AMI) is a major pathological factor implicated in the progression of ischemic heart disease (IHD). Long non-coding RNA plays an important role in regulating the occurrence and development of cardiovascular disease. The aim of this study was to investigate the regulating role of LINC00261 in hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis. The relative expression of LINC00261, miR-23b-3p and NRF2 were determined in rats I/R myocardial tissues and H/R-induced cardiomyocytes. The rat model and cell model of LINC00261 overexpression were established to investigate the biological function of LINC00261 on H9C2 cell. The interaction between LINC00261, miR-23b-3p, NRF2 and FOXO3a was identified using bioinformatics analysis, luciferase reporter assay, RNA immunoprecipitation (RIP) assay, chromatin immunoprecipitation (CHIP) assay and qRT-PCR. The expression of LINC00261 was significantly down-regulated in myocardial tissues and H9C2 cell. Overexpression of LINC00261 improves cardiac function and reduces myocardium apoptosis. Interestingly, transcription factor FOXO3a was found to promote LINC00261 transcription. Moreover, LINC00261 was confirmed as a spong of miR23b-3p and thereby positively regulates NRF2 expression in cardiomyocytes. Our findings reveal a novel role for LINC00261 in regulating H/R cardiomyocyte apoptosis and the potency of the LINC00261/miR-23b-3p/NRF2 axis as a therapeutic target for the treatment of MIRI.
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http://dx.doi.org/10.1111/jcmm.15292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412708PMC
August 2020

How low must you go? Effects of low-level noise on cochlear neural response.

Hear Res 2020 07 29;392:107980. Epub 2020 Apr 29.

Center for Hearing and Deafness, University at Buffalo, Buffalo, New York, 14214, USA.

Prolonged exposure to low-level noise has often been used scientifically as well as clinically to induce neuroplastic changes within the central auditory pathway in order to reduce central gain, suppress tinnitus and hyperacusis, and modulate different features of central auditory processing. A fundamental assumption underling these studies is that the noise exposure levels are so low that they have no effect on the neural output of the cochlea. Therefore, functional changes occurring in the central auditory pathway must be the results of central rather than peripheral changes. In an attempt to identify long-term noise exposures that did not cause peripheral changes, we measured the compound action potential (CAP) input/output functions from control rats and rats exposed for 6-weeks to 18-24 kHz noise presented at 25, 45, 55, 65, 75 or 85 dB SPL. Exposures >65 dB SPL significantly increased CAP thresholds; the critical intensity (Ct) below which no threshold shift occurred was estimated to be 55 dB SPL. Exposures >55 dB SPL significantly reduced suprathreshold CAP amplitudes; the critical intensity (Ca) below which no amplitude change was predicted to occur was a remarkably low level of 19 dB SPL. These results demonstrate that even extremely low-intensity long duration exposures can disrupt the neural output of the cochlea; these peripheral modifications are likely to contribute to the extensive compensatory changes observed at multiple levels of the central auditory pathway, neural network changes aimed at re-establishing homeostasis.
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http://dx.doi.org/10.1016/j.heares.2020.107980DOI Listing
July 2020

Impact of pulmonary thromboendarterectomy on tricuspid regurgitation in patients with chronic thromboembolic pulmonary hypertension: a single-center prospective cohort experience.

J Thorac Dis 2020 Mar;12(3):758-764

Department of Cardiovascular Surgery, China-Japan Friendship Hospital, Beijing 100029, China.

Background: For patients with chronic thromboembolic pulmonary hypertension (CTEPH) and tricuspid regurgitation (TR) undergoing pulmonary thromboendarterectomy (PTE), whether concomitant tricuspid annuloplasty should be carried out is still controversial.

Methods: The study population consisted of 45 consecutive patients with CTEPH who were scheduled to undergo PTE. All PTE surgeries were conducted with a median sternotomy and deep hypothermia circulatory arrest (DHCA). We collected and analyzed the demographics, surgical details, echocardiographic parameters, and right heart catheterization (RHC) results of these patients.

Results: Moderate to severe TR was documented in 48.9% (22/45) of the patients pre-operatively and 4.4% (2/45) of the patients post-operatively. In patients with grade 4 TR, severity decreased to grade 2 in 8 and to grade 1 in 1. In patients with grade 3 TR, severity decreased to grade 2 in 9, to grade 1 in 3, and 1 remained unchanged. In patients with grade 2 TR, severity decreased to grade 1 in 8, and 15 remained unchanged. The post-operative TR velocity was decreased significantly (431.9±53.4 196.5±154.0, P<0.001). Pulmonary artery systolic pressure was 84±17 mmHg pre-operatively and decreased to 38±14 mmHg post-operatively (P<0.001). The pre and post-operative pulmonary diastolic pressure was 29±9 and 17±7 mmHg, respectively (P<0.001). The pre and post-operative mean pulmonary pressure was 48±10 and 24±9 mmHg, respectively (P<0.001). The pulmonary vascular resistance (PVR) (1,025.4±465.0 476.6±181.2 dynes·sec·cm, P<0.001) and pulmonary artery wedge pressure (PAWP) (9±4 5±2 mmHg, P<0.001) decreased significantly after operation. The cardiac index (CI) increased significantly (1.9±0.5 2.3±0.4, P=0.003) after operation.

Conclusions: In conclusion, functional TR could be alleviated after PTE even in patients with high PVR. However, the long-term results need to be further investigated.
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http://dx.doi.org/10.21037/jtd.2019.12.99DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138973PMC
March 2020

COVID-19: Face masks and human-to-human transmission.

Influenza Other Respir Viruses 2020 07 5;14(4):472-473. Epub 2020 Apr 5.

Henan University of Chinese Medicine, Zhengzhou, Henan, China.

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http://dx.doi.org/10.1111/irv.12740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228242PMC
July 2020

RXRα and MRTF-A have a synergistic effect in the retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells.

Cell Biol Int 2020 Jun 12;44(6):1373-1381. Epub 2020 Mar 12.

Institute of Biology and Medicine, Wuhan University of Science and Technology, No. 2 Huangjiahu Road, Wuhan, Hubei, 430070, China.

Mesenchymal stem cells (MSCs) have multilineage differentiation potential and can transform into neuron cells under an appropriate environment. Retinoic acid (RA) facilitates the neuronal differentiation of MSCs. We found that RXRα, a RA receptor, was significantly upregulated in RA-induced process. Here, we show that RXRα collaborated with myocardin-related transcription factor-A (MRTF-A) to strongly promote the RA-induced process as evidenced by the increase in NF-H expression and NF-H promoter transcription activity. Our studies reveal that RXRα and MRTF-A exhibit protein interactions and synergistically inhibit the MSCs apoptosis by enhancing the P21 expression. Furthermore, RXRα and MRTF-A can activate P21 transcription by affecting the formation of the MRTF-A/RXRα/RARE complex. These findings reveal the important roles of RXRα and MRTF-A signaling in RA-induced neural-like differentiation of MSCs and describe a new mechanism underlying the synergistic interaction of RXRα and MRTF-A.
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http://dx.doi.org/10.1002/cbin.11331DOI Listing
June 2020

Functional magnetic resonance imaging of enhanced central auditory gain and electrophysiological correlates in a behavioral model of hyperacusis.

Hear Res 2020 04 6;389:107908. Epub 2020 Feb 6.

Department of Physics, City University of Hong Kong, Hong Kong, China. Electronic address:

Hyperacusis is a debilitating hearing condition in which normal everyday sounds are perceived as exceedingly loud, annoying, aversive or even painful. The prevalence of hyperacusis approaches 10%, making it an important, but understudied medical condition. To noninvasively identify the neural correlates of hyperacusis in an animal model, we used sound-evoked functional magnetic resonance imaging (fMRI) to locate regions of abnormal activity in the central nervous system of rats with behavioral evidence of hyperacusis induced with an ototoxic drug (sodium salicylate, 250 mg/kg, i.p.). Reaction time-intensity measures of loudness-growth revealed behavioral evidence of salicylate-induced hyperacusis at high intensities. fMRI revealed significantly enhanced sound-evoked responses in the auditory cortex (AC) to 80 dB SPL tone bursts presented at 8 and 16 kHz. Sound-evoked responses in the inferior colliculus (IC) were also enhanced, but to a lesser extent. To confirm the main results, electrophysiological recordings of spike discharges from multi-unit clusters were obtained from the central auditory pathway. Salicylate significantly enhanced tone-evoked spike-discharges from multi-unit clusters in the AC from 4 to 30 kHz at intensities ≥60 dB SPL; less enhancement occurred in the medial geniculate body (MGB), and even less in the IC. Our results demonstrate for the first time that non-invasive sound-evoked fMRI can be used to identify regions of neural hyperactivity throughout the brain in an animal model of hyperacusis.
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http://dx.doi.org/10.1016/j.heares.2020.107908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7080601PMC
April 2020

Pollution characteristics of phthalate acid esters in agricultural soil of Yinchuan, northwest China, and health risk assessment.

Environ Geochem Health 2020 Dec 3;42(12):4313-4326. Epub 2020 Jan 3.

School of Resources and Environment, Ningxia University, Helanshan Road 489#, Xixia District, Yinchuan, 750021, China.

Eighty-nine agricultural surface soil samples from different types of land of Yinchuan were collected and detected for sixteen phthalate acid ester (PAE) compounds; the pollution characteristics and pollution distribution were analyzed. In addition, the potential health risk exposures to local resident of six priority control phthalates by the US EPA were assessed. All soil samples were contaminated with PAEs, the total concentrations of ΣPAEs were between 0.391 and 11.924 mg kg, and the mean concentrations were 4.427 mg kg in soil. Among the sixteen PAE congeners, DMP was the most abundant component, which accounted for average 44.64% of the total PAEs, then DnBP and DEHP, which accounted for the average contribution rate, were 21.25% and 23.34%, respectively, and DpHP was not detected in all soil samples. Risk assessment indicated that the risk of non-carcinogenesis in this study was within the acceptable range; however, the carcinogenic risk of DEHP through intake dietary significantly exceeded the carcinogenic level recommended by the US EPA (1 × 10) and therefore presented a potential carcinogenic risk. More considerable attention should be given to the PAEs contamination status in soils and potential effects on local resident health.
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http://dx.doi.org/10.1007/s10653-019-00502-4DOI Listing
December 2020

High-precision and flexible calibration method of non-overlapping array cameras.

Appl Opt 2019 Nov;58(33):9251-9258

In this paper, a high-precision and flexible intrinsic and extrinsic parameters calibration method for non-overlapping array cameras is proposed. In the camera array system, each camera employs an individual planar calibration board to achieve intrinsic parameters calibration. Without assistance of any other coded or un-coded target, the extrinsic parameters of array cameras can be flexibly calibrated based on close-range photogrammetry. To overcome the disadvantages of low precision under a large field of view in close-range photogrammetry, a refinement coordinates mapping (RCM) method to achieve high-precision reconstruction of targets is developed. The principles of single-camera calibration, close-range photogrammetry, and the extrinsic calibration method are introduced, respectively. Experiments are performed to analyze the stability of single-camera calibration and the world coordinates reconstruction of targets. The results of the re-projection error in extrinsic parameters calibration show that the developed RCM method has high precision.
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http://dx.doi.org/10.1364/AO.58.009251DOI Listing
November 2019

N, P and S co-doped carbon materials derived from polyphosphazene for enhanced selective U(VI) adsorption.

Sci Total Environ 2020 Mar 9;706:136019. Epub 2019 Dec 9.

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore; Singapore Membrane Technology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore 639798, Singapore. Electronic address:

Herein, the precursor polyphosphazene was synthesized by the polymerization of hexachlorocyclotriphosphazene (HCCP) and bis(4-hydroxyphenyl) sulfone (BPS). The adsorbent which was codoped with N, P and S (amidate-CS) was developed from the precursor by using the carbonization method. The images of Scanning electron microscope (SEM) and Transmission electron microscope (TEM) indicate that the amidate-CS possessed porous graphene-like carbon lamellar structure. The excellent behaviors with respect to kinetics (120 min for equilibrium) and thermodynamics (maximum removal of 290 mg/g when pH was at 6.0) revealed the outstanding performance of amidate-CS in removing U(VI), which is due to the functional groups and strong covalent bonds between heteroatoms and uranyl ions. The adsorption of amidate-CS followed the pseudo-second-order kinetic and Langmuir adsorption model. The thermodynamic parameters indicate that the process was spontaneous and endothermic. The adsorption and desorption efficiency of amidate-CS had a slight decrease after five cycles, indicating excellent regeneration performance. Overall, the amidate-CS is a prospective candidate for highly selective U(VI) removing.
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http://dx.doi.org/10.1016/j.scitotenv.2019.136019DOI Listing
March 2020

Noise-Induced loudness recruitment and hyperacusis: Insufficient central gain in auditory cortex and amygdala.

Neuroscience 2019 12 26;422:212-227. Epub 2019 Oct 26.

Center for Hearing and Deafness, SUNY at Buffalo, Buffalo, NY 14214, USA.

Noise-induced hearing loss generally induces loudness recruitment, but sometimes gives rise to hyperacusis, a debilitating condition in which moderate intensity sounds are perceived abnormally loud. In an attempt to develop an animal model of loudness hyperacusis, we exposed rats to a 16-20 kHz noise at 104 dB SPL for 12 weeks. Behavioral reaction time-intensity functions were used to assess loudness growth functions before, during and 2-months post-exposure. During the exposure, loudness recruitment (R) was present in the region of hearing loss, but subtle evidence of hyperacusis (H) started to emerge at the border of the hearing loss. Unexpectedly, robust evidence of hyperacusis appeared below and near the edge of the hearing loss 2-months post-exposure. To identify the neural correlates of hyperacusis and test the central gain model of hyperacusis, we recorded population neural responses from the cochlea, auditory cortex and lateral amygdala 2-months post-exposure. Compared to controls, the neural output of the cochlea was greatly reduced in the noise group. Consistent with central gain models, the gross neural responses from the auditory cortex and amygdala were proportionately much larger than those from the cochlea. However, despite central amplification, the population responses in the auditory cortex and amygdala were still below the level needed to fully account for hyperacusis and/or recruitment. Having developed procedures that can consistently induce hyperacusis in rats, our results set the stage for future studies that seek to identify the neurobiological events that give rise to hyperacusis and to develop new therapies to treat this debilitating condition.
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http://dx.doi.org/10.1016/j.neuroscience.2019.09.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994858PMC
December 2019

Co-expression of cellulase and xylanase genes in toward enhanced bioethanol production from corn stover.

Bioengineered 2019 12;10(1):513-521

State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan, PR China.

Lignocellulose is considered as a good resource for producing renewable energy. Previous studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in to enhance the practical biotransformation, two recombinant strains (-CBH-CA and -CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in -CBH-CA and -CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/mL, respectively. The recombinant can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than only expressing cellulase. Fermentation with -CBH-CA and -CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than -CBH, 2.8 and 3.4 folds higher than the wild type . The strategy of co-expression cellulase and xylanase in is effective and can be foundation to research the mechanism of synergy effect of cellulose and xylanase.
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http://dx.doi.org/10.1080/21655979.2019.1682213DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844370PMC
December 2019

Altering polythiophene derivative substrates to control the electrodeposition morphology of Au particles toward ultrafine nanoparticles.

Chem Commun (Camb) 2019 Oct;55(80):12088-12091

School of Materials Science and Engineering, Beihang University, Beijing 100191, China.

A novel strategy for controlling the morphology of AuNPs by altering polythiophene derivative substrates was developed, and the nucleation mechanism of AuNPs on PTs was further explored theoretically. It is found that PTs with longer side chains can induce the electrodeposition of AuNPs with different morphologies and smaller particle sizes.
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http://dx.doi.org/10.1039/c9cc05682cDOI Listing
October 2019

Colorful Wall-Bricks with Superhydrophobic Surfaces for Enhanced Smart Indoor Humidity Control.

ACS Omega 2019 Aug 15;4(9):13896-13901. Epub 2019 Aug 15.

School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, Shanghai 200444, China.

Humidity-control materials have attracted increasing attention because of energy savings and smart regulation of indoor comforts. The current research is a successive work to face challenges, such as poor performance, limitations for large-scale production, and surface contamination. Here, we report a smart humidity-control wall-brick manufactured from sepiolite using CaCl as an additive. Low-temperature sintering generated a super hygroscopic interior structure, and further silane modification produced bricks with superhydrophobic surfaces. These superhydrophobic surfaces can promote the moisture storage and prevent the CaCl solution from leaking even after the surface is wiped 100 times. Meanwhile, the superhydrophobic surfaces make the wall-bricks easy to clean; also, these materials possess antifouling and antifungal properties. The 24 h and saturated moisture adsorption-desorption contents reached 630 and 1700 g·m, respectively. Furthermore, a test was performed using model houses in a real environment, which indicates that the wall-bricks can narrow the daily indoor humidity fluctuations by more than 20% in both wet and dry seasons. The white wall-brick can also be dyed with different colors and thus shows promise for applications in interior decorations of houses.
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http://dx.doi.org/10.1021/acsomega.9b01588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6714535PMC
August 2019

Improving Stockline Detection of Radar Sensor Array Systems in Blast Furnaces Using a Novel Encoder-Decoder Architecture.

Sensors (Basel) 2019 Aug 8;19(16). Epub 2019 Aug 8.

School of Automation & Electrical Engineering, University of Science and Technology Beijing, Beijing 100083, China.

The stockline, which describes the measured depth of the blast furnace (BF) burden surface with time, is significant to the operator executing an optimized charging operation. For the harsh BF environment, noise interferences and aberrant measurements are the main challenges of stockline detection. In this paper, a novel encoder-decoder architecture that consists of a convolution neural network (CNN) and a long short-term memory (LSTM) network is proposed, which suppresses the noise interferences, classifies the distorted signals, and regresses the stockline in a learning way. By leveraging the LSTM, we are able to model the longer historical measurements for robust stockline tracking. Compared to traditional hand-crafted denoising processing, the time and efforts could be greatly saved. Experiments are conducted on an actual eight-radar array system in a blast furnace, and the effectiveness of the proposed method is demonstrated on the real recorded data.
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http://dx.doi.org/10.3390/s19163470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719009PMC
August 2019

Preparation of porous uranium oxide hollow nanospheres with peroxidase mimicking activity: application to the colorimetric determination of tin(II).

Mikrochim Acta 2019 07 3;186(8):501. Epub 2019 Jul 3.

College of Science, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.

Porous uranium oxide hollow sphere nanoparticles were synthesized in ionic liquids under hydrothermal conditions. Various precipitating agents and ionic liquids were investigated to determine their respective impact on the resultant uranium oxide morphologies. Using hydrazine hydrate as precipitating agent and N-butyl pyridinium bromide as templating agent, a porous-hollow structure was created with a surface area of 1958 m.g and an average pore diameter of 30 nm. The nanoparticles revealed high peroxidase-mimicking activity. This was evaluated by using the peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) that is catalytically oxidized by HO to give oxidized TMB (oxTMB) which is blue (with an absorption peak at 652 nm). The material was used as a nanozyme for colorimetric detection of Sn. Meanwhile, it is found that BSA strongly improves the catalytic activity of the nanozyme, while Sn(II) inhibits its activity. Thus, a colorimetric method for Sn detection was designed. The method works in the 0.5-100 μM Sn(II) concentration range and has a lower detection limit of 0.36 μM (at S/N = 3). Graphical abstract The catalytic activity of porous-hollow nano-UO toward the oxidation of 3,3',5,5'-tetramethylbenzidine by HO is remarkably improved in the presence of bovine serum albumin, while tin(II) inhibits its activity. This finding has been applied to design a method for colorimetric quantification of tin(II) in water samples.
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http://dx.doi.org/10.1007/s00604-019-3624-1DOI Listing
July 2019

MORC2 promotes cell growth and metastasis in human cholangiocarcinoma and is negatively regulated by miR-186-5p.

Aging (Albany NY) 2019 06;11(11):3639-3649

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, P. R. China.

Microrchidia family CW-type zinc finger 2 (MORC2) is a ubiquitously expressed protein that contributes to chromatin remodeling, DNA repair, and lipogenesis. However, its role in cholangiocarcinoma (CCA) remains largely unknown. The aim of this study was to investigate the expression profile of MORC2 and its potential functions in CCA progression. The results showed that MORC2 was upregulated in human CCA specimens and cell lines. MORC2 expression was significantly associated with serum CA19-9 levels (P = 0.009), TNM stage (P = 0.003) and lymph node invasion (P = 0.004). Furthermore, high MORC2 expression was associated with poor 5-year survival (P = 0.016). Functional experiments revealed that MORC2 knockdown could suppress CCA cell proliferation, migration, and invasion both and . Mechanically, we found that MORC2 promoted CCA cell metastasis through the EMT process and enhanced proliferation via the Akt signaling pathway. Moreover, MORC2 was negatively regulated by miR-186-5p. MiR-186-5p could influence CCA cell proliferation, migration and metastasis by regulating MORC2. Taken together, the findings of this study demonstrated the oncogenic role of MORC2 in CCA tumorigenesis and metastasis, and clarified an underlying regulatory mechanism mediating MORC2 upregulation, which may provide a novel therapeutic target in CCA treatment.
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http://dx.doi.org/10.18632/aging.102003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594809PMC
June 2019