Publications by authors named "Xiaoning Li"

174 Publications

Heavy metal(loid) and Pb isotope compositions of black shale weathering profiles on the northern Yangtze Platform: insights into geochemical behavior, contamination assessment, and source apportionment.

Environ Sci Pollut Res Int 2021 May 5. Epub 2021 May 5.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 611756, Sichuan Province, China.

Heavy metal(loid) (Cd, Co, Cr, Ni, Cu, Zn, Pb, V, Tl, and As) and Pb isotope compositions of two black shale weathering profiles were determined to investigate the geochemical behaviors of these toxic elements during black shale weathering and the heavy metal(loid) contamination and source apportionment of Pb in black shale-associated soils. Black shale has higher heavy metal(loid) concentrations than the upper continental crust and the worldwide average shale. In contrast, the surface soils have much higher heavy metal(loid) concentrations than the profile soils. The heavy metal(loid) concentrations in black shale-associated soils are higher than the Chinese and worldwide soil background values, except for Co and Pb. Black shale-associated soils, especially the surface soils, have higher average concentrations of As, Cd, Cr, Ni, Cu, and Zn than Chinese, Dutch, and Canadian soil guidelines. The enrichment factor (EF) and geoaccumulation index (I) values indicate various degrees of heavy metal(loid) contamination in these soils, particularly for the heavy metals Cd, Tl, and V and metalloid As. Co and Pb contamination in these soils is not a current concern. According to the mass transfer coefficient (τ) values, Cd, Co, Ni, and Zn show overall losses, and other metals (Cr, Cu, Pb, and V) exhibit different behavior in the studied black shale weathering profiles. Based on a simple binary Pb isotopic mixing model, black shale is the dominant contributor to the Pb in black shale-associated soils (70.5-91.1% to profile soils and 81.2-88.8% to surface soils), and vehicle exhaust contributes less (8.9-29.5%) to the Pb in profile soils. Vehicle exhaust can exert an impact on the Pb isotopic evolution at depth intervals of 60-80 cm below the soil surface.
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http://dx.doi.org/10.1007/s11356-021-14234-5DOI Listing
May 2021

The Prognostic Value of PERK in Cancer and Its Relationship With Immune Cell Infiltration.

Front Mol Biosci 2021 16;8:648752. Epub 2021 Apr 16.

Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, Key Laboratory of Biomedicine in Gene Diseases, Health of Anhui Higher Education Institutes, Anhui Normal University, Wuhu, China.

Protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK) is a type I transmembrane protein that functions as an endoplasmic reticulum (ER) stress sensor to regulate global protein synthesis. Recent research studies suggest that PERK, as an important receptor protein of unfolded protein response, is involved in the pathogenesis of many cancers. This study aimed to investigate PERK expression and its relationship with prognosis in pan-cancer and attempted to explore the relevant mechanism of PERK involved in the regulation of cancer pathogenesis. The Oncomine and TIMER databases were used to analyze the expression of PERK between pan-cancer samples and normal samples. Survival analysis was performed using the PrognoScan, Kaplan-Meier (K-M) plotter, and UALCAN databases. Gene set enrichment analysis (GSEA) was used to perform the functional enrichment analysis of the PERK gene in breast invasive carcinoma (BRCA), head and neck squamous cell carcinoma (HNSC), and thyroid carcinoma (THCA). The TIMER database was used to investigate the correlation between PERK expression and tumor-infiltrating immune cells and analyze the relationship of PERK with marker genes of immune cells which were downloaded from the CellMarker database in BRCA, HNSC, and THCA. PERK was differentially expressed in various cancers, such as breast cancer, liver cancer, lung cancer, gastric carcinoma, lymphoma, thyroid cancer, leukemia, and head and neck squamous cell carcinomas. The high expression of PERK was associated with a poor prognosis in KIRP, LGG, BRCA, and THCA and with a favorable prognosis in HNSC. The results of GSEA indicated that PERK was mainly enriched in immune-related signaling pathways in BRCA, HNSC, and THCA. Moreover, PERK expression was significant positively correlated with infiltrating levels of macrophages and dendritic cells and was strongly associated with a variety of immune markers, especially macrophage mannose receptor 1 (MRC1, also called CD206) and T-helper cells (Th). The high expression of PERK could promote the infiltration of multiple immune cells in the tumor microenvironment and could deteriorate the outcomes of patients with breast and thyroid cancers, suggesting that PERK as well as tumor-infiltrating immune cells could be taken as potential biomarkers of prognosis.
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http://dx.doi.org/10.3389/fmolb.2021.648752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085429PMC
April 2021

Skin effect of facial cleansing: Should not be overemphasized compared with the long-term potential risk.

J Cosmet Dermatol 2021 Apr 30. Epub 2021 Apr 30.

Department of Dermatology, International Mongolian Hospital of Inner Mongolia, Hohhot, China.

We read with interest the study by Aiello and colleagues published online on Feb. 22. In this study, the effect of physiological properties was revealed through comparing a cleansing process with cosmetic product applied manually to cleansing with cosmetic product associated with the use of an electric sonic device and result the sonic device did not cause a significant hydration reduction with better preservation of skin homeostasis.
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http://dx.doi.org/10.1111/jocd.14190DOI Listing
April 2021

VE-cadherin-based matrix promoting the self-reconstruction of pro-vascularization microenvironments and endothelial differentiation of human mesenchymal stem cells.

J Mater Chem B 2021 Apr 6;9(15):3357-3370. Epub 2021 Apr 6.

The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Regulating the secretion and endothelial differentiation of human mesenchymal stem cells (hMSCs) plays an important role in the vascularization in tissue engineering and regenerative medicine. In this study, a recombinant cadherin fusion protein consisting of a human vascular endothelial-cadherin extracellular domain and immunoglobulin IgG Fc region (hVE-cad-Fc) was developed as a bioartificial matrix for modulating hMSCs. The hVE-cad-Fc matrix significantly enhanced the secretion of angiogenic factors, activated the VE-cadherin-VEGFR2/FAK-AKT/PI3K signaling pathway in hMSCs, and promoted the endothelial differentiation of hMSCs even without extra VEGF. Furthermore, the hVE-cad-Fc matrix was applied for the surface modification of a poly (lactic-co-glycolic acid) (PLGA) porous scaffold, which significantly improved the hemocompatibility and vascularization of the PLGA scaffold in vivo. These results revealed that the hVE-cad-Fc matrix should be a superior bioartificial ECM for remodeling the pro-vascularization extracellular microenvironment by regulating the secretion of hMSCs, and showed great potential for the vascularization in tissue engineering.
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http://dx.doi.org/10.1039/d1tb00017aDOI Listing
April 2021

Comprehensive transcriptional analysis reveals salt stress-regulated key pathways, hub genes and time-specific responsive gene categories in common bermudagrass (Cynodon dactylon (L.) Pers.) roots.

BMC Plant Biol 2021 Apr 10;21(1):175. Epub 2021 Apr 10.

Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, Shandong, People's Republic of China.

Background: Despite its good salt-tolerance level, key genes and pathways involved with temporal salt response of common bermudagrass (Cynodon dactylon (L.) Pers.) have not been explored. Therefore, in this study, to understand the underlying regulatory mechanism following the different period of salt exposure, a comprehensive transcriptome analysis of the bermudagrass roots was conducted.

Results: The transcripts regulated after 1 h, 6 h, or 24 h of hydroponic exposure to 200 mM NaCl in the roots of bermudagrass were investigated. Dataset series analysis revealed 16 distinct temporal salt-responsive expression profiles. Enrichment analysis identified potentially important salt responsive genes belonging to specific categories, such as hormonal metabolism, secondary metabolism, misc., cell wall, transcription factors and genes encoded a series of transporters. Weighted gene co-expression network analysis (WGCNA) revealed that lavenderblush2 and brown4 modules were significantly positively correlated with the proline content and peroxidase activity and hub genes within these two modules were further determined. Besides, after 1 h of salt treatment, genes belonging to categories such as signalling receptor kinase, transcription factors, tetrapyrrole synthesis and lipid metabolism were immediately and exclusively up-enriched compared to the subsequent time points, which indicated fast-acting and immediate physiological responses. Genes involved in secondary metabolite biosynthesis such as simple phenols, glucosinolates, isoflavones and tocopherol biosynthesis were exclusively up-regulated after 24 h of salt treatment, suggesting a slightly slower reaction of metabolic adjustment.

Conclusion: Here, we revealed salt-responsive genes belonging to categories that were commonly or differentially expressed in short-term salt stress, suggesting possible adaptive salt response mechanisms in roots. Also, the distinctive salt-response pathways and potential salt-tolerant hub genes investigated can provide useful future references to explore the molecular mechanisms of bermudagrass.
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http://dx.doi.org/10.1186/s12870-021-02939-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8035780PMC
April 2021

The Clouclip, a wearable device for measuring near-work and outdoor time: validation and comparison of objective measures with questionnaire estimates.

Acta Ophthalmol 2021 Mar 17. Epub 2021 Mar 17.

Aier School of Ophthalmology, Central South University, Hunan, China.

Purpose: To validate a novel wearable device that can measure both viewing distance and light exposure, Clouclip, and compare questionnaire estimates regarding near-work and outdoor time with the objective measures obtained using Clouclip.

Methods: Fifteen Clouclips were selected to measure different distances and levels of illuminance. With each Clouclip, five measurements at different distances and light intensities were measured and recorded. Eighty participants wore Clouclips for a week and completed an activity questionnaire afterwards.

Results: The intra- and inter-Clouclip coefficients were 1.00 and 0.99 for measuring distance and 1.00 and 1.00 for illuminance, respectively. Within the measurement limit, the maximum relative error was 2.07% for distance and 2.23% for illuminance. Assuming that <30 cm was the typical distance for near-work activities and >1000 Lux was the typical cut-off for outdoor environments, the questionnaire showed a trend of overestimation for both. The greatest overestimation of near-work occurred during the school period [Questionnaire: 4.73 hr (4.73, 5.07) versus Clouclip: 2.16 hr (1.74, 2.78); p < 0.01]. The greatest overestimation of outdoor activity also occurred during the school period [Questionnaire: 1.60 hr (1.33, 1.85) versus Clouclip: 1.21 hr (0.96, 1.50); p < 0.01]. Based on Clouclip, the total time spent outdoors was estimated to be 1.55 hr on school days, of which 0.34 hr occurred after school. For weekend days, however, the duration was only 0.17 hr.

Conclusions: Clouclip had excellent precision and accuracy. Although the agreement between the questionnaire and Clouclip was relatively poor, they were able to complement each other to provide a more logical and feasible assessment of exposure to near-work and outdoor activity. Indoor-oriented lifestyles were found to predominate in Chinese children.
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http://dx.doi.org/10.1111/aos.14785DOI Listing
March 2021

Exogenous Enhances Drought and Heat Tolerance of Perennial Ryegrass.

Front Microbiol 2021 19;12:593722. Epub 2021 Feb 19.

Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, China.

Perennial ryegrass () is a cool-season grass whose growth and development are limited by drought and high temperature. has been reported to promote plant growth and counteract the adverse effects of abiotic stresses. The objective of this study was to assess -induced response mechanisms to drought and heat resistance in perennial ryegrass. We evaluated the physiological and biochemical markers of drought and heat stress based on the hormone homeostasis, photosynthesis, antioxidant enzymes activity, lipid peroxidation, and genes expression level. We found out that under drought and heat stress, -inoculated leaves exhibited higher abscisic acid (ABA) and lower salicylic acid (SA) contents than non-inoculated regimes. In addition, under drought and heat stress, the fungus enhanced the photosynthetic performance, decreased the antioxidase activities, and mitigated membrane lipid peroxidation compared to non-inoculated regime. Furthermore, under drought stress, induced a dramatic upregulation of and and a downregulation of , , and genes. In addition, under heat stress, -inoculated plants exhibited a higher expression level of , , and while a lower expression level of and than non-inoculated ones. Our results provide an evidence of the protective role of in perennial ryegrass response to drought and heat stresses.
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http://dx.doi.org/10.3389/fmicb.2021.593722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933552PMC
February 2021

Hydrogen Generation and Degradation of Organic Dyes by New Piezocatalytic 0.7BiFeO-0.3BaTiO Nanoparticles with Proper Band Alignment.

ACS Appl Mater Interfaces 2021 Mar 26;13(9):11050-11057. Epub 2021 Feb 26.

Institute for Superconducting and Electronic Materials, Australian Institute for Innovative Materials, University of Wollongong, North Wollongong, New South Wales 2500, Australia.

Piezoelectric materials have recently demonstrated their potential applications in clean energy exploration and environmental remediation through triggering a number of catalytic reactions by harvesting waste vibrational energy in the environment. In this work, unique lead-free 0.7BiFeO-0.3BaTiO (BF-BT) nanoparticles with tuned band structure were synthesized by the hydrothermal method for use as piezoelectric catalysts to generate hydrogen by splitting water; a high production rate of 1.322 mmol/g was achieved in 1 h, which is 10 times higher than the production rate of pure BiFeO. Of particular interest, BF-BT particles attached to nickel mesh have the ability to degrade rhodamine B in flowing water, demonstrating their potential to treat polluted water by anchoring BF-BT in drains. Finally, we propose novel insight on the piezocatalytic mechanism, which is based on the internal electric field (the sum of the depolarization field and the screening charge field) that drives electron/hole separation and movement.
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http://dx.doi.org/10.1021/acsami.1c01407DOI Listing
March 2021

Enhanced Tendon-to-Bone Healing via IKKβ Inhibition in a Rat Rotator Cuff Model.

Am J Sports Med 2021 03 28;49(3):780-789. Epub 2021 Jan 28.

Department of Biomedical Engineering, Columbia University, New York, New York, USA.

Background: More than 450,000 rotator cuff repairs are performed annually, yet healing of tendon to bone often fails. This failure is rooted in the fibrovascular healing response, which does not regenerate the native attachment site. Better healing outcomes may be achieved by targeting inflammation during the early period after repair. Rather than broad inhibition of inflammation, which may impair healing, the current study utilized a molecularly targeted approach to suppress IKKβ, shutting down only the inflammatory arm of the nuclear factor κB (NF-κB) signaling pathway.

Purpose: To evaluate the therapeutic potential of IKKβ inhibition in a clinically relevant model of rat rotator cuff repair.

Study Design: Controlled laboratory study.

Methods: After validating the efficacy of the IKKβ inhibitor in vitro, it was administered orally once a day for 7 days after surgery in a rat rotator cuff repair model. The effect of treatment on reducing inflammation and improving repair quality was evaluated after 3 days and 2, 4, and 8 weeks of healing, using gene expression, biomechanics, bone morphometry, and histology.

Results: Inhibition of IKKβ attenuated cytokine and chemokine production in vitro, demonstrating the potential for this inhibitor to reduce inflammation in vivo. Oral treatment with IKKβ inhibitor reduced NF-κB target gene expression by up to 80% compared with a nontreated group at day 3, with a subset of these genes suppressed through 14 days. Furthermore, the IKKβ inhibitor led to enhanced tenogenesis and extracellular matrix production, as demonstrated by gene expression and histological analyses. At 4 weeks, inhibitor treatment led to increased toughness, no effects on failure load and strength, and decreases in stiffness and modulus when compared with vehicle control. At 8 weeks, IKKβ inhibitor treatment led to increased toughness, failure load, and strength compared with control animals. IKKβ inhibitor treatment prevented the bone loss near the tendon attachment that occurred in repairs in control.

Conclusion: Pharmacological inhibition of IKKβ successfully suppressed excessive inflammation and enhanced tendon-to-bone healing after rotator cuff repair in a rat model.

Clinical Relevance: The NF-κB pathway is a promising target for enhancing outcomes after rotator cuff repair.
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http://dx.doi.org/10.1177/0363546520985203DOI Listing
March 2021

Clinical Analysis of Bloodstream Infections During Agranulocytosis After Allogeneic Hematopoietic Stem Cell Transplantation.

Infect Drug Resist 2021 19;14:185-192. Epub 2021 Jan 19.

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Purpose: To explore the epidemiological characteristics and risk factors of bloodstream infections (BSI) in patients who develop agranulocytosis fever after allogeneic hematopoietic stem cell transplantation (allo-HSCT). This study also provides a basis for the clinical treatment of BSI.

Methods: A retrospective analysis of 397 allo-HSCT patients in the Department of Hematology of our hospital was conducted from January 2013 to December 2017 to analyze the incidence of BSI, the distribution and types of pathogenic bacteria, and drug resistance rates. We also determined whether various parameters are risk factors to BSI, including the patient age, gender, disease type, transplantation method, stem cell source, pre-treatment with anti-thymocyte globulin (ATG), and agranulocytosis time.

Results: Among the 397 allo-HSCT patients, 294 had a fever during the period of agranulocytosis, and 52 cases were found to have BSI. The incidence of BSI in patients with agranulocytosis fever was 17.7% (52/294). Among the 60 pathogens detected, 43 (71.67%), 10 (16.67%), and 7 (11.67%) were Gram negative strains, Gram positive strains, and fungi, respectively. The most common bacteria were , and . The detection rate of extended-spectrum β-lactamase (ESBL) was 40.0%, and carbapenem-resistant (CRE) accounted for 17.9%. Single-factor and multi-factor analyses showed that pre-treatment with ATG, agranulocytosis time (≥21 days), and stem cell source were risk factors for BSI.

Conclusion: We found that in our hospital, BSIs in allo-HSCT patients are mainly caused by Gram-negative bacteria, and the resistance rate to carbapenem drugs is high. Pre-treatment with ATG, agranulocytosis time (≥21 days), and stem cell source are risk factors for BSI.
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http://dx.doi.org/10.2147/IDR.S280869DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826046PMC
January 2021

Resveratrol alleviates bleomycin-induced pulmonary fibrosis via suppressing HIF-1α and NF-κB expression.

Aging (Albany NY) 2021 01 20;13(3):4605-4616. Epub 2021 Jan 20.

Department of Respiratory Medicine, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, China.

The absence of a gold standard for treating pulmonary fibrosis makes its management challenging. We established a rat model to study the effect of resveratrol (Res) on bleomycin-induced pulmonary fibrosis. Rats were randomly divided into control, model, low-Res, middle-Res, high-Res, and dexamethasone groups and treated with various concentrations of these drugs. Rats showed typical features of pulmonary fibrosis; i.e., alveolitis, fibrous hyperplasia, and fibrosis on days 7, 14, and 28, respectively. Expression of HIF-1α and NF-κB was higher in the middle-Res, high-Res, and dexamethasone groups than in the control group, but was less than that in the model and low Res groups. We conclude that different levels of HIF-1α and NF-κB expression at different stages of pulmonary fibrosis in rats is positively correlated with the disease severity. Furthermore, resveratrol alleviates bleomycin-induced pulmonary fibrosis by suppressing HIF-1α and NF-κB expression, indicating its potential as a promising therapeutic drug candidate.
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http://dx.doi.org/10.18632/aging.202420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906133PMC
January 2021

Effect of book-shaped acellular tendon scaffold with bone marrow mesenchymal stem cells sheets on bone-tendon interface healing.

J Orthop Translat 2021 Jan 24;26:162-170. Epub 2020 Mar 24.

Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, 410008, People's Republic of China.

Background: Tissue engineering has exhibited great effect on treatment for bone-tendon interface (BTI) injury. The aim of this study was to evaluate the effect of a book-shaped acellular tendon scaffold (ATS) with bone marrow mesenchymal stem cells sheets (MSCS) for BTI injury repair.

Methods: ATS was designed based on the shape of "book", decellularization effect was evaluated by Hematoxylin and eosin (H&E), 4', 6-diamidino-2-phenylindole (DAPI) and scanning electron microscopy (SEM), then bone marrow mesenchymal stem cells (MSCs) were cultured on ATS to assess the differentiation inductivity of ATS. A rabbit right partial patellotomy model was established, and MSCS seeded on ATS were implanted into the lesion site. The patella-patellar tendon (PPT) at 2, 4, 8 or 16 weeks post-operation were obtained for histological, biomechanical and immunofluorescence analysis.

Results: H&E, DAPI and SEM results confirmed the efficiency of decellularization of ATS, and their in vitro tenogenic and chondrogenic ability were successfully identified. In vivo results showed increased macrophage polarization toward the M2 phenotype, IL-10 expression, regenerated bone and fibrocartilage at the patella-patellar tendon interface of animals received MSCS modified ATS implantation. In addition, the level of tensile strength was also the highest in MSCS modified ATS implantation group.

Conclusion: This study suggests that ATS combined with MSCS performed therapeutic effects on promoting the regeneration of cartilage layer and enhancing the healing quality of patella-patellar tendon interface.

The Translational Potential Of This Article: This study showed the good biocompatibility of the ATS, as well as the great efficacy of ATS with MSCS on tendon to bone healing. The results meant that the novel book-shaped ATS with MSCS may have a great potential for clinical application.
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http://dx.doi.org/10.1016/j.jot.2020.02.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7773951PMC
January 2021

Gold nanoclusters modified mesoporous silica coated gold nanorods: Enhanced photothermal properties and fluorescence imaging.

J Photochem Photobiol B 2021 Feb 26;215:112111. Epub 2020 Dec 26.

MicroNano System Research Center, College of Information and Computer & Key Laboratory of Advanced Transducers and Intelligent Control System of Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address:

Photothermal therapy (PTT) is a non-invasive therapy that is widely used in cancer treatment. Gold nanorods (AuNRs) are particularly suitable as a photothermal reagent due to their unique localized surface plasmon resonance (LSPR) properties. However, bare gold nanorods are not stable enough during radiation to collect enough energy to kill tumor cells. In addition, they showed some biologically toxic originated from the poor colloidal stability and surfactants cetyltrimethyl ammonium bromide (CTAB), making it difficult to apply them directly to clinical research. To solve these problems, a novel nanocomposite was structured by coating silica shell and gold nanocluster on the outer layer of the gold nanorod (AuNRs@SiO@AuNCs). Compared with the bare gold nanorod, the nanocomposite with the core-shell structure showed superior photothermal effect. The photothermal conversion temperature reached 63 °C under a lower irradiation power. The photothermal conversion efficiency was enhanced to 77.6%. Its photothermal performance remained constant after five cycles of near-infrared laser irradiation, indicating excellent photothermal stability. In vitro cell imaging experiments show that AuNRs@SiO@ AuNCs can effectively enter tumor cells. By 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis, cancer cells can be effectively killed when exposed to a near-infrared laser. During the synthesis process, the silica and gold nanoclusters replaced the toxic CTAB molecular layer on the surface of AuNRs. Therefore, AuNRs@SiO@AuNCs has good biocompatibility and fluorescence characteristics. These results suggest that such AuNRs@SiO@AuNCs nanocomposite shows great potential in imaging guided photothermal therapy for cancer.
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http://dx.doi.org/10.1016/j.jphotobiol.2020.112111DOI Listing
February 2021

lncRNA LIFR‑AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR‑4698.

Mol Med Rep 2021 02 23;23(2). Epub 2020 Dec 23.

Department of Surgery, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.

The vital functions of long non‑coding (lnc)RNAs have been verified in gastric carcinoma (GC). However, as a novel cancer‑related lncRNA, the influence of leukemia inhibitory factor receptor antisense RNA 1 (LIFR‑AS1) in GC cell biological behaviors remains unreported. The present study explored the biological effects of lncRNA LIFR‑AS1 on GC progression. Reverse transcription‑quantitative PCR was performed to examine lncRNA LIFR‑AS1 expression in GC tissues and cells. Cell Counting Kit‑8, 5‑ethynyl‑2'‑deoxyuridine incorporation, cell wound healing and Transwell invasion assays were used to assess the functions of lncRNA LIFR‑AS1 in GC cell proliferation, migration and invasion. Additionally, associations among lncRNA LIFR‑AS1, microRNA (miR)‑4698 and microtubule‑associated tumor suppressor 1 (MTUS1) were investigated via bioinformatics software and a luciferase reporter system. In addition, western blotting was used to examine the expression of MEK and ERK. Decreased lncRNA LIFR‑AS1 expression was observed in GC tissues and cells. Upregulated lncRNA LIFR‑AS1 inhibited GC cell proliferation, migration and invasion. Upregulated miR‑4698 and downregulated MTUS1 were identified in GC tissues and cells. The inhibitory interaction between lncRNA LIFR‑AS1 and miR‑4698 was confirmed. Additionally, MTUS1 was predicted as a target gene of miR‑4698 positively regulated by lncRNA LIFR‑AS1. The MEK/ERK pathway was inhibited by lncRNA LIFR‑AS1 via regulating MTUS1. These findings revealed the inhibitory functions of lncRNA LIFR‑AS1 in GC cell proliferation, migration and invasion. The process was mediated via miR‑4698, MTUS1 and the MEK/ERK pathway.
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http://dx.doi.org/10.3892/mmr.2020.11792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789130PMC
February 2021

Moderately low nitrogen application mitigate the negative effects of salt stress on annual ryegrass seedlings.

PeerJ 2020 3;8:e10427. Epub 2020 Dec 3.

Coastal Salinity Tolerant Grass Engineering and Technology Research Center, Ludong University, Yantai, China.

Appropriate application of nitrogen (N) can alleviate the salt stress-induced damage on plants. This study explores the changes of nitrogen requirement in feeding annual ryegrass seedlings under mild salt concentrations (50 mM, 100 mM) plus its underlying mitigation mechanism. Results showed that low salt concentration decreased N requirement as observed from the increment in plant height and biomass at a relative low N level (2.0 mM not 5.0 mM). Under salt treatment, especially at 50 mM NaCl, the OJIP (Chl a fluorescence induction transient) curve and a series of performance indexes (PI, RC/CS, ET/CS, ϕE, ϕ) peaked whereas DI/RC, V and M were the lowest under moderately low N level (2.0 mM). In addition, under salt stress, moderately low N application could maintain the expression of NR (nitrate reductase) and GS (glutamine synthetase) encoding genes at a relatively stable level but had no effect on the expression of detected NRT (nitrate transporter) gene. The seedlings cultured at 2.0 mM N also exhibited the highest activity of CAT and POD antioxidant enzymes and the lowest MDA content and EL under relative low level of salt treatment. These results indicated that mild salt treatment of annual ryegrass seedlings might reduce N requirement while moderately low N application could promote their growth via regulating photosynthesis, alleviating ROS-induced (reactive oxygen species) damage and maintenance of N metabolism. These results also can provide useful reference for nitrogen application in moderation rather than in excess on annual ryegrass in mild or medium salinity areas through understanding the underlying response mechanisms.
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http://dx.doi.org/10.7717/peerj.10427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719293PMC
December 2020

Prognostic nutritional index predicts acute kidney injury and mortality of patients in the coronary care unit.

Exp Ther Med 2021 Feb 3;21(2):123. Epub 2020 Dec 3.

Department of Ultrasound Imaging, Renmin Hospital of Wuhan University, Wuhan, Hubei 430061, P.R. China.

The current study aimed to investigate whether prognostic nutritional index (PNI) is an independent predictor of acute kidney injury (AKI) and mortality of patients in the coronary care unit (CCU). In the present two-stage observational study of patients in the CCU, 6,444 patients from the Medical Information Mart for Intensive Care (MIMIC) III database were first enrolled (test cohort), after which 412 patients from Zhongnan Hospital of Wuhan University were recruited in the validation cohort. AKI was defined based on the Kidney Disease Improving Global Outcomes AKI criteria. The primary endpoint was the incidence of AKI stratified by severity, while the second endpoint included in-hospital mortality and 2-year mortality. In the test cohort, 4,457 (69.2%) patients developed AKI during hospitalization. Following multivariable adjustment, the highest quartile of the PNI value was associated with a 1.8-fold increased risk of AKI compared with the lowest quartile. For the prediction of AKI, the area under the receiver operating characteristic curve outperformed the acute physiology score III score and clinical model in patients with or without preexisting chronic kidney disease, and this was further validated in the hospital cohort used in the present study. A total of 2,219 patients suffered mortality during the 2-year follow-up, and PNI was indicated to independently predict the risk of in-hospital mortality and 2-year mortality in the test cohort and in the validation cohort. Decision curve analysis indicated that the PNI values were clinically useful; Therefore, the current study demonstrated that the PNI value is an independent predictor of AKI and mortality in patients within the CCU.
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http://dx.doi.org/10.3892/etm.2020.9555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739862PMC
February 2021

High Oxygen Evolution Activity of Tungsten Bronze Oxides Boosted by Anchoring of Co at Nb Sites Accompanied by Substantial Oxygen Vacancy.

Adv Sci (Weinh) 2020 Nov 29;7(22):2002242. Epub 2020 Sep 29.

Institute for Superconducting & Electronic Materials (ISEM) Australia Institute for Innovative Materials Innovation Campus University of Wollongong Squires Way North Wollongong NSW 2500 Australia.

The participation of lattice oxygen in the oxygen evolution reaction (OER) process has been proved to be faster in kinetics than the mechanisms where only metal is involved, although activating the lattice oxygen in the traditional rigid structures remains a big challenge. In this work, efforts are devoted to exploring a new flexible structure that is competent in providing large amounts of oxygen vacancies as well as offering the freedom to manipulate the electronic structure of metal cations. This is demonstrated by anchoring low valence state Co at high valence state Nb sites in the tetragonal tungsten bronze (TTB)-structured SrBaNb Co O , with different ratios of Co to Nb to optimize the Co substitution proportion. It is found that the occupation of Co in the Nb sites gives rise to the generation of massive surface oxygen vacancies (O), while Co itself is stabilized in Co by adjacent O. The coexistence of O and LS Co enables an oxygen intercalation mechanism in the optimal SBNC45 with specific activity at 1.7 V versus reversible hydrogen electrode that is 20 times higher than for the commercial IrO. This work illuminates an entirely new avenue to rationally design OER electrocatalysts with ultrafast kinetics.
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http://dx.doi.org/10.1002/advs.202002242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675188PMC
November 2020

Carbapenemase OXA-423: A Novel OXA-23 Variant in .

Infect Drug Resist 2020 10;13:4069-4075. Epub 2020 Nov 10.

Department of Clinical Laboratory, Yijishan Hospital of Wannan Medical College, Wuhu, People's Republic of China.

Purpose: A novel variant of OXA-23, named OXA-423, was identified in an clinical isolate. The aim of this study was to analyse the resistance phenotype of OXA-423.

Methods: The strain WY-0713 was isolated from an intensive care unit patient. PCR was used to detect the -like genes. Amplifying, cloning and sequencing were performed for the complete -like. The novel and its ancestor were cloned into the expression vector pET-28b(+), and transformed into Rosetta (DE3) for antibiotic susceptibility testing. SDS-PAGE, modified Hodge test and CarbaNP test were used for detecting the expression of OXA-423 and OXA-23.

Results: PCR screening of WY-0713 was positive for -like genes. Sequencing of the PCR product identified a novel  named which encoding  OXA-423. OXA-423 differed from OXA-23 by a crucial amino acid substitution (Val128Ala). The V128A substitution was located at the conserved active-site motifs SAV of OXA-23. Antibiotic susceptibility testing performed using isogenic showed that the MICs of Rosetta (pET-OXA-423) for penicillins and carbapenems were lower (reduced MICs 4-fold to 16-fold) than that of Rosetta (pET-OXA-23). The MICs of cefotaxime, ceftazidime and aztreonam for both transformants remained the same as the acceptor strain. Moreover, OXA-423 was slightly inhibited by sulbactam, clavulanic acid and tazobactam. SDS-PAGE analysis showed that OXA-423 and OXA-23 were conspicuously expressed. Modified Hodge test and CarbaNP test were positive demonstrated both of them were functional.

Conclusion: OXA-423, the first report of an amino acid substitution located at conserved active-site motifs of OXA-23, conferred lower MIC values of penicillins and carbapenems as compared with OXA-23, while without affecting the resistance profiles of expanded-spectrum cephalosporins and aztreonam.
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http://dx.doi.org/10.2147/IDR.S277364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7666985PMC
November 2020

Downregulation of USP12 inhibits tumor growth via the p38/MAPK pathway in hepatocellular carcinoma.

Mol Med Rep 2020 Dec 1;22(6):4899-4908. Epub 2020 Oct 1.

School of Laboratory Medicine, Wannan Medical College, Wuhu, Anhui 241002, P.R. China.

Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. Ubiquitin‑specific protease 12 (USP12) is specifically upregulated in the tumor tissues of patients with HCC compared with the corresponding adjacent normal tissues. However, the relationship between USP12 and the growth of HCCs is not fully understood. In the present study, USP12 was knocked down in HCC cell lines to investigate its effects on proliferation and apoptosis. The results showed that USP12‑knockdown could inhibit the proliferation and promote apoptosis in HCC cell lines. Flow cytometry analysis also showed that USP12 could induce cell cycle arrest at the G2/M stage. In vivo experiments showed that USP12‑knockdown could suppress tumor growth in mice, and immuno‑blotting revealed that USP12 could induce G2/M arrest through the cyclin dependent kinase 1/cyclinB1 axis, and trigger apoptosis via the p38/mitogen‑activated protein kinase pathway. These data strongly indicate that USP12 is a potential target for the treatment of HCC.
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http://dx.doi.org/10.3892/mmr.2020.11557DOI Listing
December 2020

Impaired sperm quantity and motility in adult rats following gestational and lactational exposure to environmentally relevant levels of PBDE-47: A potential role of thyroid hormones disruption.

Environ Pollut 2021 Jan 6;268(Pt A):115773. Epub 2020 Oct 6.

Department of Occupational and Environmental Health, MOE Key Laboratory of Environment and Health, State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, China. Electronic address:

Polybrominated diphenyl ethers (PBDEs) are flame retardants and the congener 2, 2', 4, 4'-tetrabromodiphenyl ether (PBDE-47) is capable of inducing thyroid endocrine disruption and developmental toxicity. However, little is known about whether developmental PBDE-47 exposure-elicited alterations in semen quality is associated with thyroid hormones (THs) perturbation. In this research, we sought to explore the impacts of gestational and lactational PBDE-47 exposure on adult sperm quantity and motility, and its link with THs levels. For this purpose, female Sprague-Dawley rats were administered environmentally relevant PBDE-47 levels (0.1, 1.0, 10 mg/kg/day) by oral gavage from prepregnancy through lactation cessation to achieve early-life exposure of offspring and to mimic the actual exposure. Sperm quantity and motility together with serum THs levels from male offspring were determined on postnatal day 88. In utero and lactational exposure to PBDE-47 boosted the weight gain while reduced the relative testis weight in adult male offspring. These were accompanied with the reductions in sperm counts (total and living sperm counts), the percentage of progressive sperm motility, sperm velocities (curvilinear velocity, straight-line velocity and average path velocity), motion path (beat cross frequency, linearity and wobble) and linear motile sperm parameters (count, motility and concentration). Further studies identified that the levels of serum triiodothyronine (T) were increased by PBDE-47 exposure and negatively associated with those differential semen parameters on quantity and motility. Collectively, our results indicate that exposure to low-level PBDE-47 during early-life development impairs semen quality in adult rats, which could be mediated partially by abnormal T levels.
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http://dx.doi.org/10.1016/j.envpol.2020.115773DOI Listing
January 2021

Elucidation of distinct fluorescence and room-temperature phosphorescence of organic polymorphs from benzophenone-borate derivatives.

Phys Chem Chem Phys 2020 Sep;22(37):21445-21452

Institute of Molecular Aggregation Science, Tianjin University, Tianjin, 300110, P. R. China.

The development of organic room-temperature phosphorescence (RTP) is accompanied by opportunities and challenges. RTP from crystal polymorphism has aroused much attention, due to the significant different photophysical characteristics and intermolecular packings found in the same molecule with different crystal phases. Herein, we report three organic molecules BP-o-BO, BP-m-BO, and BP-p-BO, in which two crystal polymorphisms of BP-p-BO are successfully cultivated with different emission properties. BP-p-BO-A exhibits bright cyan photoluminescence (PL) with a quantum yield of 11.3% and a distinct RTP with a lifetime of 17.1 ms, which is much higher than the deep blue PL of BP-p-BO-B (6.9%) and the corresponding RTP lifetime of 3.3 ms. Crystal structure analyses indicate that the different emission properties can be ascribed to the different intermolecular packing, further demonstrating the essential role of molecular packing in the designing of RTP materials.
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http://dx.doi.org/10.1039/d0cp02881aDOI Listing
September 2020

Influence of contest on Chinese college students' health literacy based on repeated cross-sectional studies.

J Am Coll Health 2020 Sep 14:1-8. Epub 2020 Sep 14.

Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing, China.

Objective: Chinese college students' level of health literacy is low, so, we explored the effect of knowledge contests on health literacy (HL) among Chinese college students. Students from universities in Jiangsu, China. Methods: Two repeated cross-sectional studies were used to compare the effects of the college students' contests. Chi-square tests and variance analysis were used to compare the HL levels and scores, respectively; logistic regression was used for multivariate analysis. The levels of HL after each contest were significantly higher than those in the baseline survey ( < .05). There were statistically significant score differences ( = .023 and  = .001) after the contests in the two studies. Multivariable analysis of HL contest showed that school, grade, profession, completion time, and contest history were statistically significant. HL among Chinese college students was low and it is helpful to regularly hold health knowledge contests to improve these levels, especially for most non-medical students.
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http://dx.doi.org/10.1080/07448481.2020.1807991DOI Listing
September 2020

MiR-199-3p modulates the onset of puberty in rodents probably by regulating the expression of Kiss1 via the p38 MAPK pathway.

Mol Cell Endocrinol 2020 12 18;518:110994. Epub 2020 Aug 18.

Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, Donghua University, Shanghai, China; College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai, China. Electronic address:

The Kiss1 gene plays an indispensable role in modulating the onset of puberty and fertility in mammals. Although an increasing number of genetic and environmental factors that influence reproduction through Kiss1 have been identified, the function of microRNAs, a class of posttranscriptional regulators, in regulating Kiss1 expression remains poorly understood. This study aimed at investigating the mechanism by which Kiss1 expression is regulated by microRNAs. A simplified miRNome screen by a dual-fluorescence reporter system based on Kiss1 was performed to identify microRNAs that affect the expression of Kiss1. The expression patterns of the identified microRNAs during the period of murine sexual development were investigated, and only miR-199-3p was studied further. Aided by bioinformatics algorithms, miR-199-3p was demonstrated to be a repressor of Kiss1 expression, as it blocked the expression of Kiss1 through the p38 MAPK pathway by simultaneously inhibiting several targets in both GT1-7 cells and primary hypothalamic neurons. Both the inhibition of the p38 MAPK pathway by the intracerebroventricular administration of chemical agents in rats and the ectopic expression of miR-199-3p by lentivirus injection in the hypothalamus in mice delayed puberty onset and gonad development. Our results presented a novel regulatory mechanism of puberty onset which the sustained downregulation of miR-199-3p might gradually release the inhibition of the p38 MAPK/Fos/CREB/Kiss1 pathway during puberty development.
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http://dx.doi.org/10.1016/j.mce.2020.110994DOI Listing
December 2020

Efficacy and Mechanism of Butylphthalide Combined with Atorvastatin Calcium Tablets in the Diagnosis of Cerebral Infarction Using Iodol/Fe₃O₄ Nanometric Contrast Agent.

J Nanosci Nanotechnol 2020 Dec;20(12):7356-7361

Nuclear Industry 215 Hospital of Shaanxi Province, Xianyang 712000, Shaanxi, PR China.

To determine serum proteolytic activity, NO (nitric oxide) levels, TAC (total antioxidant capacity), and TOS (total oxidant status) in acute hemorrhagic stroke, the oxidative stress response mechanism of atorvastatin was studied in a rat model with cerebral infarction. All patient contrast agents are Iodol/Fe₃O₄ nanometric contrast agents. Serum prolinease activity, catalase activity, NO levels, TAC, and TOS were spectrophotometrically determined and the OSI (oxidative stress index) calculated. In this study, the serum TAC level and catalase activity with acute hemorrhagic stroke were significantly lower than in the control group (each = 0.001), while the level of NO, TOS, OSI, and protease were significantly higher than in the control group ( = 0.01). When patients were classified for acute hemorrhagic stroke by gender, there was no difference in serum prolinease activity, meanwhile, NO, TAC, and TOS levels, and OSI differed between men and women (as mentioned with details acknowledged by all, = 0.05). The results of this study indicate that compared with the control group, elevated oxidative stress levels, decreased antioxidants levels, and elevated prolinease activity are correlated with acute hemorrhagic stroke. Additionally, further research is needed to elucidate the mechanisms of oxidative stress with acute hemorrhagic stroke.
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http://dx.doi.org/10.1166/jnn.2020.18605DOI Listing
December 2020

Psychological impact of healthcare workers in China during COVID-19 pneumonia epidemic: A multi-center cross-sectional survey investigation.

J Affect Disord 2020 09 19;274:405-410. Epub 2020 May 19.

Zhongnan Hospital of Wuhan University, Wuhan City, Hubei Province, People's Republic of China. Electronic address:

Background: Since the outbreak of 2019 new coronavirus (COVID-19) pneumonia, healthcare workers (HCW) have suffered psychological stress. The present study is to examine the prevalence of stress, anxiety and depression of HCW in China during the COVID-19 epidemic, and to determine the risk factors predicting psychological morbidities that can be used as psychological intervention targets.

Methods: A cross-sectional survey was conducted to investigate the psychological levels of HCW in multiple centers in China. The prevalence of stress, anxiety and depression were determined by using Perceived Stress Scale (PSS-14) and Hospital Anxiety / Depression scale (HAD). Psychology related factors were evaluated and correlation between job title and contact history was analyzed.

Results: We received 958 of effective responses, 73.6% of which were from Wuhan and 67.2% were female participants. 55.1% of respondents had psychological stress that is higher than that of HCW during SARS. 54.2% and 58% of participants had symptoms of anxiety and depression. Stress levels of HCW were different in job titles and years of work experience. Anxiety and depression levels were different between different gender, job titles, degrees of protective measures and levels of contact history. Gender, intermediate title, protective measures and contact history were the independent risk factors for anxiety. Protective measures and contact history were the independent risk factors for depression.

Conclusions: The COVID-19 epidemic has induced stress levels for HCW, and high percentages of HCW have anxiety and depression. The situation of HCW is worrying and intervention service is urgent.
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http://dx.doi.org/10.1016/j.jad.2020.05.081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236675PMC
September 2020

Cellulose-based adsorbents loaded with zero-valent iron for removal of metal ions from contaminated water.

Environ Sci Pollut Res Int 2020 Sep 12;27(26):33234-33247. Epub 2020 Jun 12.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NB, E3B 5A3, Canada.

Sawdust loaded with zero-valent iron (S-ZVI) was prepared using a liquid phase reduction method for removing heavy metal ions from contaminated water. Surface chemistry and morphology of adsorbents were characterized with Fourier transform infrared (FT-IR) spectrometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), SEM-mapping, EDX, and X-ray photoelectron spectrum (XPS). The results demonstrated that the zero-valent iron was successfully loaded onto the sawdust. The impact of various factors such as pH, initial metal ion concentration, temperature, and contact time on the removal capability of the adsorbents was systematically investigated. The equilibrium adsorption data showed that the adsorption of arsenic ions and Cr(III) followed the Langmuir model well, and the maximum adsorption reached 111.37 and 268.7 mg/g in an aqueous solution system. In addition, the adsorption kinetics was more accurately described by the pseudo-second-order model, suggesting the domination of chemical adsorption. Meanwhile, the results on recyclability indicated that the high performance of S-ZVI on the removal of arsenic ions was well maintained after three regeneration cycles. The adsorption mechanism revealed in this work suggested that S-ZVI improved the dispersion of ZVI by minimizing the agglomeration, thus leading to highly effective adsorption via chelation, electrostatic interaction, and redox reaction.
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http://dx.doi.org/10.1007/s11356-020-09390-zDOI Listing
September 2020

Two-Dimensional, High-Resolution Peripheral Refraction in Adults with Isomyopia and Anisomyopia.

Invest Ophthalmol Vis Sci 2020 06;61(6):16

,.

Purpose: The purpose of this study was to investigate the two-dimensional peripheral refraction in fellow eyes of patients with isomyopia and anisomyopia.

Methods: Sixty-eight young adults were recruited, including 25 isomyopes with interocular differences (IODs) of foveal refraction < 1.00 D and 43 anisomyopes with IOD > 1.50 D. Peripheral refraction across an area of the visual field of 60° × 36° with a resolution of 1° was measured using a custom-made Hartmann-Shack wavefront sensor. The retinal area was divided into 3 × 3 zones for comparison between the fellow eyes.

Results: There was no difference of refraction in all corresponding zones between the fellow eyes in the isomyopic group (all P > 0.05). The IODs between more myopic (MM) eyes and less myopic (LM) eyes in the anisomyopic group ranged from -1.40 to approximately -2.46 D (all P <0.001), which was flagged in the center and attenuated in peripheral zones by varied magnitudes. In the stratification analysis for different levels of anisomyopia, the nasal retina first presented significant relative hyperopic shifts compared to the center, followed by the temporal retina. In contrast, the superior and inferior periphery only differed from the center when the central IOD was greater than 3.00 D.

Conclusions: The two-dimensional peripheral refraction patterns showed a mirror symmetry between the fellow eyes of a patient with isomyopia. However, in the anisomyopic group, peripheral refraction showed significantly relative hyperopic shift when compared with the center and developed with a varied rate in different areas. These findings may indicate an asymmetrical variation in the peripheral refraction patterns during myopia progression.
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http://dx.doi.org/10.1167/iovs.61.6.16DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415287PMC
June 2020

The Efficacy of New Chinese Diabetes Risk Score in Screening Undiagnosed Type 2 Diabetes and Prediabetes: A Community-Based Cross-Sectional Study in Eastern China.

J Diabetes Res 2020 29;2020:7463082. Epub 2020 Apr 29.

Department of Health Education, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China.

The New Chinese Diabetes Risk Score (NCDRS) is one of the recommended tools for screening undiagnosed type 2 diabetes in China. However, its performance in detecting undiagnosed diabetes needs to be verified in different community populations. Also, it is unknown whether NCDRS can be used in detecting prediabetes. In the present study, we aimed to evaluate the performance of NCDRS in detecting undiagnosed diabetes and prediabetes among the community residents in eastern China. We applied NCDRS in 7675 community residents aged 18-65 years old in Jiangsu Province. The results showed that the participants with undiagnosed diabetes reported the highest NCDRS value, followed by those with prediabetes ( < 0.001). The best cut-off points of NCDRS for detecting undiagnosed diabetes and prediabetes were 27 (with a sensitivity of 78.0% and a specificity of 57.7%) and 27 (with a sensitivity of 66.0% and a specificity of 62.9%). The AUCs of NCDRS for identifying undiagnosed diabetes and prediabetes were 0.749 (95% CI: 0.739~0.759) and 0.694 (95% CI: 0.683~0.705). These results demonstrate the excellent performance of NCDRS in screening undiagnosed diabetes in the community population in eastern China and further provide evidence for using NCDRS in detecting prediabetes.
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http://dx.doi.org/10.1155/2020/7463082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7210548PMC
March 2021

WIPI2 depletion inhibits the growth of hepatocellular carcinoma cells through the AMPK signaling pathway.

Oncol Rep 2020 May 4;43(5):1467-1478. Epub 2020 Mar 4.

Department of Hepatobiliary Surgery, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.

WD‑repeat domain phosphoinositide‑interacting protein 2 (WIPI2) is a protein that regulates the assembly of multiprotein complexes by presenting a beta‑propeller platform for simultaneous and reversible protein‑protein interactions. This study was designed to investigate the association between the expression of WIPI2 and the growth of hepatocellular carcinoma (HCC). Publicly‑available data from the UALCAN platform revealed that WIPI2 is upregulated in tumor tissues compared with that noted in normal tissues in many types of tumors especially in HCC, and high WIPI2 expression predicts a poor patient prognosis. WIPI2 expression was significantly higher in tumor tissues compared with that in the corresponding adjacent normal tissues. Depletion of WIPI2 inhibited the proliferation and promoted the apoptosis both in HCC Huh7 and Hep3B cells. In order to explore the mechanisms of WIPI2 in HCC, WIPI2 was depleted in HCC cell lines and a gene microarray was constructed. The bioinformatic analysis showed that WIPI2 regulated the proliferation of HCC cells mainly through the AMPK signaling pathway. Further analysis indicated that the downstream factors of the AMPK signaling pathway were downregulated after WIPI2 depletion. Collectively, our study revealed that WIPI2 plays an important role in the pathogenesis of HCC mainly through the AMPK signaling pathway.
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http://dx.doi.org/10.3892/or.2020.7531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107944PMC
May 2020

A report of clinical diagnosis and treatment of nine cases of coronavirus disease 2019.

J Med Virol 2020 06 16;92(6):683-687. Epub 2020 Mar 16.

Department of Clinical Laboratory, The First Affiliated Hospital of Wanan Medical College, Wuhu, Anhui, China.

Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 has become an important public health issue in the world. More than 118 000 cases were confirmed around the world. The main clinical manifestations were respiratory symptoms and occasional gastrointestinal symptoms. However, there is no unified standard for the diagnosis and treatment of COVID-19. In the retrospective analysis, we report nine cases of COVID-19, describe the history of contact, clinical manifestations, the course of diagnosis and clinical treatment before, during and after treatment.
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http://dx.doi.org/10.1002/jmv.25755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7228316PMC
June 2020