Publications by authors named "Xiaoming Wu"

300 Publications

Visual Performance Following Bilateral Implantation of Refractive Rotationally Asymmetric Bifocal Intraocular Lens (LS-313 MF30) or Apodized Diffractive Bifocal Intraocular Lens (ReSTOR SN6AD1).

Ther Clin Risk Manag 2021 1;17:917-926. Epub 2021 Sep 1.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, People's Republic of China.

Purpose: This study compared the clinical outcomes after cataract surgery with implantation of refractive rotationally asymmetric bifocal intraocular lens (IOL) (LS-313 MF30) and apodized diffractive bifocal IOL (ReSTOR SN6AD1).

Methods: This was a prospective, non-randomized, controlled study, where patients diagnosed with age-related cataracts were selected for phacoemulsification combined with bilateral IOL implantation. Based on the type of IOL voluntarily implanted, the patients were divided into two groups, ie, refractive and diffractive groups. In total, 30 cases (60 eyes) were in a refractive group, while 30 cases (60 eyes) were in diffractive group. Three months after surgery, we examined the uncorrected distance visual acuity (UDVA), uncorrected intermediate visual acuity (UIVA), uncorrected near visual acuity (UNVA), defocus curve, objective visual quality, and subjective questionnaire.

Results: Three months after surgery, the UIVA of the refractive group (0.18 ± 0.08) logMAR was better than that of the diffractive group (0.29 ± 0.16) logMAR (P < 0.05). No significant difference in UDVA and UNVA was noted between the two groups. For a 4mm pupil diameter, the intraocular and total eye aberration, higher-order aberration (HOA), coma, spherical aberration, and trefoil in the refractive group were significantly higher than those in diffractive group (P < 0.05). The intraocular modulation transfer function (MTF), intraocular strehl ratio (SR), total eye MTF, and total eye SR in the refractive group were lower than those in diffractive group (P < 0.05). No significant difference in glare incidence, spectacle independence rate, and patient satisfaction was observed between the two groups (P > 0.05). The halos incidence in the refractive group was lower than the diffractive group (P < 0.05).

Conclusion: Both bifocal IOLs obtained satisfactory UDVA and UNVA, with higher patient satisfaction. Unlike the apodized diffractive bifocal IOL, the refractive rotationally asymmetric bifocal IOL yielded slightly better UIVA, lower halos incidence, whereas the apodized diffractive bifocal IOL showed a better objective visual quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/TCRM.S325287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420797PMC
September 2021

Case Report: Rare Pulmonary Sclerosing Pneumocytoma: Large, Multiple, Metastatic, and Fatal.

Front Med (Lausanne) 2021 16;8:661032. Epub 2021 Aug 16.

Department of Thoracic Surgery, Henan Provincial Chest Hospital, Zhengzhou, China.

Pulmonary sclerosing pneumocytoma (PSP) is a rare benign or low-grade malignant tumor, but it has the potential to present with multiple lesions, lymph node metastasis, extra-pulmonary metastasis, recurrence and even cause death. Herein, a case of PSP that was huge, presented with multiple lesions and had lymph node as well as extrapulmonary metastases (liver, abdominal cavity, bones) is reported for the first time. This patient was also the first one to die of respiratory and circulatory failure caused by the PSP tumor and its metastases which compressed the mediastinal tissue.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.661032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415542PMC
August 2021

Temporal and Spatial Characteristics of Cataract Surgery Rates in China.

Risk Manag Healthc Policy 2021 25;14:3551-3561. Epub 2021 Aug 25.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, People's Republic of China.

Objective: The aim of the current study was to explore the spatial and temporal distribution characteristics of registered cases of cataract surgery in China from 2013 to 2017.

Methods: A database for spatial analysis of cataract surgery in China in 2013-2017 was established using ArcGIS10.0 software as a platform for data management and presentation. Spatial autocorrelation analysis of cataract surgery was undertaken, and temporal and spatial scan analysis was done using SaTScan 9.5 software.

Results: From 2013 to 2017, annual cataract surgery rates (CSRs) in China were 1200, 1400, 1782, 2070, and 2205 per 1 million population, indicating a gradually increasing trend. Local Moran's autocorrelation analysis showed that there was spatial clustering of CSR in China, with Anhui being a low-high clustering region. Findings of global hotspot analysis Getis-Ord General showed that General index of national CSR was <0.01, = 1.12, = 0.26. Findings of staged spatial-temporal scan analysis indicated that 18 areas of aggregation were found in 2 stages. Observed differences in each clustering area were statistically significant ( < 0.05).

Conclusion: CSRs in China showed increasing trend year by year and were randomly distributed, with spatial clustering, and Anhui was reported as a low-high clustering region. However, high-risk areas still persist, requiring focused attention and targeted prevention and control measures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/RMHP.S317547DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405972PMC
August 2021

Coexistent Integer Charge Transfer and Charge Transfer Complex in F4-TCNQ-Doped PTAA for Efficient Flexible Organic Light-Emitting Diodes.

J Phys Chem Lett 2021 Sep 31;12(35):8533-8540. Epub 2021 Aug 31.

Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education; Tianjin Key Laboratory of Photoelectric Materials and Devices; National Demonstration Center for Experimental Function Materials Education; School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China.

Understanding the mechanism of interaction between organic polymers and dopants is of great significance to further enhance the performances of flexible electronics. Here, the two doping mechanisms of charge transfer complex (CTC) and integer charge transfer (ICT) are found to coexist in p-π conjugated PTAA doped with the strong acceptor F4-TCNQ, and their correlation is affected by the HJ-aggregate state of the doped polymer. The growth of the J-aggregate caused by the increase of CTC would lead to a corresponding formation of ICT. The doping efficiency was dominated by the CTC/ICT ratio. On the basis of the analysis of the optical, electrical, and morphological properties of PTAA:F4-TCNQ films, we optimized the CTC/ICT ratio to achieve the efficient hole transport layers that are used in solution-processed flexible phosphorescent organic light-emitting diodes with p-i-n structure. The optimal device presents a very high current efficiency (CE) of 31.12 cd/A and a low turn-on voltage of 3.6 V.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.1c02281DOI Listing
September 2021

Functional Status of Pancreatic and Cells in Type 2 Diabetes Mellitus Patients with Different Plasma Triglyceride Levels: A Retrospective Analysis.

Int J Endocrinol 2021 17;2021:9976067. Epub 2021 Aug 17.

NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.

Objective: To investigate the functional status of pancreatic and cells in Type 2 diabetes mellitus (T2DM) patients with different plasma triglyceride (TG) levels. TG levels can be prognostic markers for T2DM.

Methods: A total of 328 patients with T2DM were divided into three groups according to different TG levels: the TGL group: TG < 1.7 mmol/L; TGM group: 1.7 mmol/L ≤ TG < 2.3 mmol/L; and TGH group: TG ≥ 2.3 mmol/L. An oral glucose tolerance test (OGTT), insulin release test, and glucagon release test were performed in each patient. The changes of glucagon, glucagon/insulin ratio, early insulin secretion index (Δ /Δ ), and area under the insulin curve (AUC) were compared among each group. Also, the correlations between glucagon and pancreatic -cell function, glycosylated hemoglobin (HbA1c), and other indices were analyzed.

Results: With the increase of TG, the fasting and postprandial glucagon levels, the glucagon/insulin ratio, and the area under the glucagon curve (AUC) presented an increasing trend. The homeostasis model assessment of insulin resistance (HOMA-IR) of the TGH group was significantly increased compared to the TGL and TGM groups. In addition to the increase in TG levels, the insulin sensitivity index (ISI), homeostasis model assessment for -cell function index (HOMA-), Δ /Δ , and AUC displayed a reducing trend. Glucagon was negatively correlated with Δ /Δ , high-density lipoprotein (HDL), HOMA-, body mass index (BMI), ISI, and AUC ( < 0.05) and positively correlated with fasting blood glucose (FPG), AUC, HOMA-IR, HbA1c, duration, TG, low-density lipoprotein (LDL), and total cholesterol (TC) ( < 0.05).

Conclusion: Hypertriglyceridemia aggravated the dysfunction of pancreatic and cells. A reasonable control of the TG level makes it easier for blood glucose to reach the standard.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/9976067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387189PMC
August 2021

Neutrophil extracellular traps contribute to tissue plasminogen activator resistance in acute ischemic stroke.

FASEB J 2021 Sep;35(9):e21835

Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.

Circulating neutrophil extracellular traps (NETs) resistant to t-PA have not been studied completely although NETs in thrombi may contribute to tissue plasminogen activator (t-PA) resistance. This research intended to elucidate whether circulating NETs are associated with t-PA resistance and the underlying mechanism. The levels of NETs were detected in the circulating neutrophils, ischemic brain tissue of acute ischemic stroke (AIS) patients, and transient middle cerebral artery occlusion (tMCAO) models. NET formation in blood, thrombi, and ischemic brain tissue of mice were analyzed by immunofluorescence. Exposed phosphatidylserine (PS) was assessed using flow cytometry and confocal microscopy. Procoagulant activity (PCA) was evaluated using fibrin formation assays, thrombin, and purified coagulation complex. The plasma levels of NETs in AIS patients were significantly higher than those in healthy individuals. After thrombolysis, a significant increase was noted in NET markers in no-improvement patients, while the changes in improvement patients were not significant. Importantly, NETs were decorated with von Willebrand factor (vWF) and plasminogen activator inhibitor-1 (PAI-1) in the blood and thrombi, which could reverse the fibrinolytic effects. In addition, NETs activated platelets (PLTs) and endothelial cells (ECs), stimulating a procoagulant phenotype and facilitating vWF and PAI-1 release. DNase I, activated protein C (APC), and sivelestat markedly inhibited these effects. Furthermore, targeting NETs protected mice from tMCAO-induced cerebral ischemia, possibly by regulating vWF and PAI-1. In summary, NETs may contribute to t-PA resistance in AIS through activation of PLTs and ECs. Strategies against NETs may present a promising therapeutic approach to improve the thrombolysis efficiency of t-PA in AIS patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1096/fj.202100471RRDOI Listing
September 2021

Sustainable self-compacting concrete containing high-amount industrial by-product fly ash as supplementary cementitious materials.

Environ Sci Pollut Res Int 2021 Aug 14. Epub 2021 Aug 14.

Jiangsu Transportation Research Institute, Nanjing, 211112, Jiangsu, China.

Nowadays, utilizing large amount industrial by-product fly ash (FA) as the alternatives for cement in self-compacting concrete (SCC) had attracted more attention. In this study, FA was employed in SCC at five levels (0 %, 20 %, 30 %, 40 %, 50 %). The mechanical behaviors, the water porosities, the transport properties, and the sustainability of FA series SCC were investigated. At the initial curing stage (3 days), the use of FA in SCC reduces mechanical properties and increases water porosity, water absorption and water absorption coefficient (sorptivity) of SCC. FA series SCC have the lower resistance against carbon dioxide attack and chloride ion penetration than cement-based SCC. The prolonging curing time is beneficial to improve the long-term behaviors of FA- blended SCC. After SCC made by 20 %, 30 %, and 40 % FA water-curing for 90 days, there are the reduction of 0.44-2.09 % in the mechanical behaviors and the increase of 0.082-0.41 % in the water porosity, compared to pure-cement SCC. Beyond the content of FA (40 %), the difference s of the mechanical properties and the water porosity between SCC with 50 % FA and fully cement SCC are below the value of 2.5 %. With the progress in the curing time, the largest reduction rates of the water absorption and the sorptivity in all SCC mixtures were found in 50 % FA-blended SCC. Utilizing 50 % FA in SCC reduces the total charge passed values of SCC. The manufacture of 50 % FA-blended SCC has the lowest energy consumption and released amounts of CO, NO, and SO in all series SCC mixtures. The application of high-level FA to SCC is the positive assistance to prepare sustainable SCC with satisfying long-term behaviors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-15883-2DOI Listing
August 2021

BN/BO-Ullazines and Bis-BO-Ullazines: Effect of BO Doping on Aromaticity and Optoelectronic Properties.

J Org Chem 2021 Sep 1;86(18):12507-12516. Epub 2021 Aug 1.

Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin Key Laboratory of Drug Targeting and Bioimaging, School of Chemistry and Chemical Engineering, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, People's Republic of China.

We have achieved substitutional doping of ullazine with either two BO units or with one BO unit and one BN unit. The synthesis of these B-doped ullazines is straightforward, using demethylation and borylative cyclization as the key steps. Ullazine cores of both BN/BO-ullazines () and bis-BO-ullazines () are very close to being planar. Their electronic and photophysical properties were investigated by ultraviolet-visible, fluorescence spectroscopy, cyclic voltammetry, and density functional theory calculations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.joc.1c00777DOI Listing
September 2021

Calunduloside E inhibits HepG2 cell proliferation and migration via p38/JNK-HMGB1 signalling axis.

J Pharmacol Sci 2021 Sep 24;147(1):18-26. Epub 2021 May 24.

Department of Biochemistry and Molecular Biology, PR China; Anhui Province Key Laboratory of Active Biological Macro-molecules, PR China. Electronic address:

High-mobility group box 1 (HMGB1), a highly conserved chromosome protein, is considered as a potential therapeutic target and novel biomarker because of its regulation in the proliferation and metastasis of Hepatocellular carcinoma (HCC). Calenduloside E (CE), a natural active product, has been reported to anti-cancer effect. However, the role and underlying molecular mechanism of CE in HCC is still unclear. The purpose of this study is to investigate the effects of CE on the proliferation and migration of HCC, and then explore the possible underlying molecular mechanism. HepG2 cells were treated with CE or transfected with HMGB1 shRNA plasmids, EdU and colony formation assays were used to detect cell proliferation ability. Wound healing and transwell assays were used to determine the role of CE in cell migration. The expression of Cyclins, PCNA, MMPs, HMGB1, N-cadherin, E-cadherin and phosphorylation of p38, ERK and JNK were all detected using Western blotting. Our results showed that CE inhibited HepG2 cells proliferation and migration in a dose dependent manner; reduced the expression levels of Cycins, PCNA, HMGB1, MMPs and N-cadherin; up-regulated E-cadherin expression; enhanced the phosphorylation of p38 and JNK signalling pathways. Blocking the activation of p38 and JNK obviously reversed CE-mediated inhibitory effects on HepG2 cell proliferation and migration; reversed CE-induced down-regulation of Cyclins, PCNA, MMPs, N-cadherin and HMGB1, as well as E-cadherin up-regulation. In conclusion, our study suggested that CE reduces the expression levels of Cyclins, MMPs and epithelial-mesenchymal transformation (EMT) through p38/JNK-HMGB1 signaling axis and then inhibits HepG2 cells proliferation and migration in HepG2 cells. This study provides a new perspective for the anti-tumour molecular mechanism of CE in HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jphs.2021.05.005DOI Listing
September 2021

Asymmetric epigenetic modification and homoeolog expression bias in the establishment and evolution of allopolyploid Brassica napus.

New Phytol 2021 Jul 15. Epub 2021 Jul 15.

College of Life Sciences, Wuhan University, Wuhan, 430072, China.

This study explores how allopolyploidization reshapes the biased expression and asymmetric epigenetic modification of homoeologous gene pairs, and examines the regulation types and epigenetic basis of expression bias. We analyzed the gene expression and four epigenetic modifications (DNA methylation, H3K4me3, H3K27me3 and H3K27ac) of 29 976 homoeologous gene pairs in resynthesized, natural allopolyploid Brassica napus and an in silico 'hybrid'. We comprehensively elucidated the biased gene expression, asymmetric epigenetic modifications and the generational transmission characteristics of these homoeologous gene pairs in B. napus. We analyzed cis/trans effects and the epigenetic basis of homoeolog expression bias. There was a significant positive correlation between two active histone modifications and biased gene expression. We revealed that parental legacy was the dominant principle in the remodeling of homoeolog expression bias and asymmetric epigenetic modifications in B. napus, and further clarified that this depends on whether there were differences in the expression/epigenetic modifications of gene pairs in parents/progenitors. The maternal genome was dominant in the homoeolog expression bias of resynthesized B. napus, and this phenomenon was attenuated in natural B. napus. Furthermore, cis rather than trans effects were dominant when epigenetic modifications potentially affected biased expression of gene pairs in B. napus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.17621DOI Listing
July 2021

TMSB4 Overexpression Enhances the Potency of Marrow Mesenchymal Stromal Cells for Myocardial Repair.

Front Cell Dev Biol 2021 9;9:670913. Epub 2021 Jun 9.

Department of the Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.

Objective: The actin-sequestering proteins, thymosin beta-4 (Tβ4) and hypoxia-inducible factor (HIF)-1α, are known to be associated with angiogenesis after myocardial infarction (MI). Herein, we aimed to identify the mechanism of HIF-1α induction by Tβ4 and investigate the effects of bone marrow mesenchymal stromal cells (BMMSCs) transfected with the Tβ4 gene () in a rat model of MI.

Methods: Rat BMMSCs were isolated, cultured, and transfected with the gene by using the lentivirus-mediated method. Rats with surgically induced MI were randomly divided into three groups ( = 9/group); after 1 week, the rats were injected at the heart infarcted border zone with TMSB4-overexpressed BMMSCs (BMMSC-TMSB4 ), wild-type BMMSCs that expressed normal levels of TMSB4 (BMMSC-TMSB4 ), or medium (MI). The fourth group of animals ( = 9) underwent all surgical procedures necessary for MI induction except for the ligation step (Sham). Four weeks after the injection, heart function was measured using transthoracic echocardiography. Infarct size was calculated by TTC staining, and collagen volume was measured by Masson staining. Angiogenesis in the infarcted heart area was evaluated by CD31 immunofluorescence histochemistry. experiments were carried out to observe the effect of exogenous Tβ4 on HIF-1α and explore the various possible mechanism(s).

Results: experiments showed that vascular density 4 weeks after treatment was about twofold higher in BMMSC-TMSB4 -treated animals than in BMMSC-TMSB4 -treated animals ( < 0.05). The cardiac function and infarct size significantly improved in both cell-treatment groups compared to controls. Notably, the cardiac function and infarct size were most prominent in BMMSC-TMSB4 -treated animals (both < 0.05). HIF-1α and phosphorylated HIF-1α (p-HIF-1α) were significantly enhanced by exogenous Tβ4, which was nonetheless blocked by the factor-inhibiting HIF (FIH) promoter (YC-1). The expression of prolyl hydroxylase domain proteins (PHD) was decreased upon treatment with Tβ4 and further decreased with the combined treatment of Tβ4 and FG-4497 (a specific PHD inhibitor).

Conclusion: TMSB4-transfected BMMSCs might significantly improve recovery from myocardial ischemia and promote the generation of HIF-1α and p-HIF-1α the AKT pathway, and inhibit the degradation of HIF-1α the PHD and FIH pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.670913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221609PMC
June 2021

LncRNA GAS8-AS1 downregulates lncRNA NEAT1 to inhibit glioblastoma cell proliferation.

Brain Behav 2021 06 4;11(6):e02128. Epub 2021 May 4.

Department of Neurology, People's Hospital of Deyang City, Deyang, China.

Background: LncRNA GAS8-AS1 has been reported to participate in several types of cancer, while its role in glioblastoma (GBM) is unknown. In the present study, we aimed to investigate the function of GAS8-AS1 in GBM and the underlying mechanisms.

Methods: The expression levels of GAS8-AS1 and NEAT1 in GBM patients and the healthy controls were measured by performing RT-qPCR. Diagnostic values of plasma GAS8-AS1 and NEAT1 for GBM were analyzed by performing ROC curve analysis with GBM patients as true positive cases and the healthy controls as true negative cases. Linear regression analysis was performed to study the correlation between the expression levels of GAS8-AS1 and NEAT1. The expression levels of GAS8-AS1 and NEAT1 in GBM cells were also determined by RT-qPCR. CCK-8 and transwell invasion assays were performed to detect the proliferation and invasion of GBM cells. Western blot assay was performed to detect the expression levels of β-catenin, Axin2, c-myc, cyclin D1, and GAPDH in GBM cells.

Results: GAS8-AS1 was downregulated, while lncRNA NEAT1 was upregulated in the plasma of GBM patients. Altered expression levels of GAS8-AS1 and NEAT1 distinguished GBM patients from the healthy controls. The expression of GAS8-AS1 and NEAT1 was inversely correlated only in GBM patients. Overexpression of GAS8-AS1 reduced the expression levels of NEAT1 in GBM cells, while knock-down of GAS8-AS1 increased the expression levels of NEAT1. However, overexpression of NEAT1 showed no significant effects on the expression of GAS8-AS1. Knock-down of GAS8-AS1 promoted GBM cell proliferation and invasion and enhanced the activation of the Wnt/β-catenin pathway. However, the effects of knock-down of GAS8-AS1 were alleviated by the knock-down of NEAT1.

Conclusion: Overexpression of GAS8-AS1 inhibits GBM cell proliferation and invasion by downregulating NEAT1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/brb3.2128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213648PMC
June 2021

Neutrophil extracellular traps induced by pro-inflammatory cytokines enhance procoagulant activity in NASH patients.

Clin Res Hepatol Gastroenterol 2021 Apr 10:101697. Epub 2021 Apr 10.

Department of Hematology, The First Affiliated Hospital, Harbin Medical University, Harbin Heilongjiang, China; Department of Research, VA Boston Healthcare System, Harvard Medical School, Boston, MA, USA; Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 1400 VFW Parkway, West Roxbury, Boston, MA 02132, USA. Electronic address:

Background: Nonalcoholic steatohepatitis (NASH) patients are at a high risk of developing venous thromboembolism, with a high rate of morbidity and mortality. The role of neutrophil extracellular traps (NETs) in procoagulant activity (PCA) in patients with NASH remains unclear. Our study aimed to investigate the formation of NETs in NASH patients stimulated by specific pro-inflammatory factors. Moreover, we evaluated the pivotal role of NETs in the induction of hypercoagulability in NASH and the interaction between NETs and endothelial injury.

Method: The levels of the NETs biomarkers were evaluated in the plasma samples of 27 NASH patients and 18 healthy subjects. The formation of NETs was visualized using immunofluorescence microscopy. The PCA of the NETs was assessed using coagulation time, purified coagulation complex, and fibrin formation assays. Confocal microscopy was further used to evaluate the interactions between the NETs and HUVECs.

Results: The levels of NETs markers in the plasma of NASH patients were significantly higher than healthy controls. NETs derived from NASH enhanced thrombin and fibrin formation and significantly reduced CT (p<0.05). The mixture of IL-6 and TNF-α triggered the NETs release in the plasma rather than them alone. Additionally, the NETs exerted cytotoxic effects on the endothelial cells, converting them to a procoagulant and pro-inflammatory phenotype, and DNase I could reverse these effects.

Conclusion: Our results revealed the primary role of NETs in promoting the hypercoagulable state in NASH patients. Methods that prevent the formation of NETs may be a novel approach for the prevention and treatment of NASH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.clinre.2021.101697DOI Listing
April 2021

Greener Solution to Waste Corn Stalks and Shortage of Asphalt Resource: Hydrochar Produced by Hydrothermal Carbonization as a Novel Performance Enhancer for Asphalt Binder.

Materials (Basel) 2021 Mar 15;14(6). Epub 2021 Mar 15.

School of Civil Engineering and Transportation, South China University of Technology, Guangzhou 510641, China.

Utilization of waste corn stalks (CS) has seized extensive attention due to high annual output and hazardous impact of piling aside or direct combustion on environment. However, previously there has been a lot of emphasis on improvement of its energy efficiency as solid fuel while limited investigations are available which explore the possibility of applying corn stalks as performance enhancer in asphalt binder. The purpose of this study is to examine the potential of employing hydrochar as modifiers in asphalt binder by a series of experimental tests. In this study, two hydrochar were produced from corn stalks by a novel process called hydrothermal carbonization at a different reaction temperature. The two hydrochar and their responding hydrochar-modified asphalt (HCMA) were tested by chemical and rheological tests. Chemical analysis detected the interaction between hydrochar and binder factions, resulting in poor compatibility but satisfying anti-aging property. Even though hydrochar increased the viscosity of bitumen, implying worse workability, and caused poor storage stability, ameliorated performance of asphalt binder at high temperature by incorporating hydrochar was verified by various criteria such as higher performance grade (PG) failure temperature and lower non-recoverable creep compliance (). Moreover, higher reaction temperature makes hydrochar's particles smaller and more homogeneous, which results in slightly lower enhanced high temperature performance, more satisfying workability, better storage stability, and greater anti-aging effect of hydrochar-modified asphalt. Eventually, this study provided a promising win-win solution to environment problems concerning corn stalk treatment and shortage of asphalt binder. Further exploration of methods to improve HCMA's storage stability, real-scale corroboration on trial section and life cycle assessment of asphalt pavement containing hydrochar modifiers will be followed in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14061427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001865PMC
March 2021

Acute Infectious Endophthalmitis After Cataract Surgery: Epidemiological Characteristics, Risk Factors and Incidence Trends, 2008-2019.

Infect Drug Resist 2021 25;14:1231-1238. Epub 2021 Mar 25.

Qingdao Eye Hospital of Shandong First Medical University, Qingdao, 266071, People's Republic of China.

Objective: Intended to investigate the epidemiological characteristics, risk factors and incidence trend of endophthalmitis after cataract surgery in recent 12 years.

Methods: A retrospective study was conducted on the patients who underwent cataract surgery in Qingdao Eye Hospital from January 1, 2008, to December 31, 2019, including age, sex, history of diabetes, intraoperative operation and complications. In addition, the related risk factors and incidence trend of acute infective endophthalmitis were analyzed. The incidence of endophthalmitis and its related factors were analyzed by univariate and multivariate logistic regression analysis.

Results: A total of 55,612 cases of cataract surgery were performed in our hospital in 12 years, and 42 cases of acute infective endophthalmitis occurred (the incidence rate was 0.076%). The average occurrence time was 10.57 days ± 11.17, with eye pain as the main complaint and anterior chamber fibrin exudation as the main clinical manifestation. In addition, there were eight cases of anterior chamber hypopyon and 18 cases of vitreous reaction, the results of anterior aqueous humor or vitreous fluid culture in 10 patients were positive. Univariate analysis showed that intraoperative rupture of posterior capsule, non-use of antibiotics and non-use of behind-the-lens washout were the risk factors of infectious endophthalmitis after cataract surgery (P=0.032, P=0.000, P=0.000). Besides, multivariate logistic regression analysis showed that intraoperative rupture of posterior capsule was the main risk factor.

Conclusion: The occurrence of endophthalmitis after cataract surgery is mainly related to intraoperative rupture of posterior capsule, absence of antibiotics and behind-the-lens washout. In addition, the incidence of endophthalmitis after cataract surgery decreased during the past 12 years.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S304675DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007477PMC
March 2021

Nitrogen utilisation-efficient oilseed rape (Brassica napus) genotypes exhibit stronger growth attributes from flowering stage onwards.

Funct Plant Biol 2021 07;48(8):755-765

College of Natural Resource and Environment, Northwest A and F University, 712100 Yangling, Shaanxi, China; and Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, 712100 Yangling, Shaanxi, China; and Corresponding author. Email:

Preliminary studies observed a lower growth activity during the vegetative stage with higher growth attributes at the pod-filling stage among the high nitrogen (N) utilisation efficiency (NUtE) oilseed rape (Brassica napus L.) genotypes, compared with the low NUtE genotypes. Therefore, we hypothesised that there would exist a critical growth stage when distinctive phenotypic traits are exhibited to regulate yield formation and NUE. A field experiment and a hydroponic culture were conducted to characterise the differences in shoot and root physiological indicators of the high and low NUtE oilseed rape genotypes at seedling, bud, bolting, flowering and pod-filling stages. We found that flowering was the critical period when the reverse growth habit occurred between high and low NUtE genotypes. The high NUtE genotypes displayed larger values of root traits, stronger N uptake kinetics parameters, higher activity of leaf glutamine synthetase (GS) and glutamate synthetase (GOGAT), larger SPAD values and net photosynthetic rate, ultimately leading to higher seed yield and NUE. Our results indicate that flowering is the critical growth stage to distinguish the high from low NUtE oilseed rape genotypes, and plant breeders may focus on selecting root and shoot phenotypic traits from flowering stage onwards to achieve both high yields and NUE for oilseed rape genotypes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1071/FP20263DOI Listing
July 2021

Complete chloroplast genome features and phylogenetic analysis of Eruca sativa (Brassicaceae).

PLoS One 2021 12;16(3):e0248556. Epub 2021 Mar 12.

Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.

Eruca sativa Mill. (Brassicaceae) is an important edible vegetable and a potential medicinal plant due to the antibacterial activity of its seed oil. Here, the complete chloroplast (cp) genome of E. sativa was de novo assembled with a combination of long PacBio reads and short Illumina reads. The E. sativa cp genome had a quadripartite structure that was 153,522 bp in size, consisting of one large single-copy region of 83,320 bp and one small single-copy region of 17,786 bp which were separated by two inverted repeat (IRa and IRb) regions of 26,208 bp. This complete cp genome harbored 113 unique genes: 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Forty-nine long repetitive sequences and 69 simple sequence repeats were identified in the E. sativa cp genome. A codon usage analysis of the E. sativa cp genome showed a bias toward codons ending in A/T. The E. sativa cp genome was similar in size, gene composition, and linearity of the structural region when compared with other Brassicaceae cp genomes. Moreover, the analysis of the synonymous (Ks) and non-synonymous (Ka) substitution rates demonstrated that protein-coding genes generally underwent purifying selection pressure, expect ycf1, ycf2, and rps12. A phylogenetic analysis determined that E. sativa is evolutionarily close to important Brassica species, indicating that it may be possible to transfer favorable E. sativa alleles into other Brassica species. Our results will be helpful to advance genetic improvement and breeding of E. sativa, and will provide valuable information for utilizing E. sativa as an important resource to improve other Brassica species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248556PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7954331PMC
March 2021

Optimized Culture Conditions for Improved Growth and Functional Differentiation of Mouse and Human Colon Organoids.

Front Immunol 2020 12;11:547102. Epub 2021 Feb 12.

Immunology Pharmacology, AbbVie, AbbVie Bioresearch Center, Worcester, MA, United States.

Background & Aims: Diligent side-by-side comparisons of how different methodologies affect growth efficiency and quality of intestinal colonoids have not been performed leaving a gap in our current knowledge. Here, we summarize our efforts to optimize culture conditions for improved growth and functional differentiation of mouse and human colon organoids.

Methods: Mouse and human colon organoids were grown in four different media. Media-dependent long-term growth was measured by quantifying surviving organoids imaging and a cell viability readout over five passages. The impact of diverse media on differentiation was assessed by quantifying the number of epithelial cell types using markers for enterocytes, stem cells, Goblet cells, and enteroendocrine cells by qPCR and histology upon removal of growth factors.

Results: In contrast to Wnt3a-conditioned media, media supplemented with recombinant Wnt3a alone did not support long-term survival of human or mouse colon organoids. Mechanistically, this observation can be attributed to the fact that recombinant Wnt3a did not support stem cell survival or proliferation as demonstrated by decreased LGR5 and Ki67 expression. When monitoring expression of markers for epithelial cell types, the highest level of organoid differentiation was observed after combined removal of Wnt3a, Noggin, and R-spondin from Wnta3a-conditioned media cultures.

Conclusion: Our study defined Wnt3a-containing conditioned media as optimal for growth and survival of human and mouse organoids. Furthermore, we established that the combined removal of Wnt3a, Noggin, and R-spondin results in optimal differentiation. This study provides a step forward in optimizing conditions for intestinal organoid growth to improve standardization and reproducibility of this model platform.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2020.547102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906999PMC
May 2021

The Distinct Function of p21 With p16 in Modulating Aging Phenotypes of Werner Syndrome by Affecting Tissue Homeostasis.

Front Genet 2021 5;12:597566. Epub 2021 Feb 5.

Laboratory of Molecular Genetics of Aging & Tumor, Medical School, Kunming University of Science and Technology, Kunming, China.

Human Werner syndrome (WS) is an autosomal recessive progeria disease. A mouse model of WS manifests the disease through telomere dysfunction-induced aging phenotypes, which might result from cell cycle control and cellular senescence. Both p21 (p21, encoded by the gene) and p16 (p16, encoded by the gene) are cell cycle inhibitors and are involved in regulating two key pathways of cellular senescence. To test the effect of p21 and p16 deficiencies in WS, we crossed WS mice (DKO) with or mice to construct triple knockout (p21-TKO or p16-TKO) mice. By studying the survival curve, bone density, regenerative tissue (testis), and stem cell capacity (intestine), we surprisingly found that p21-TKO mice displayed accelerated premature aging compared with DKO mice, while p16-TKO mice showed attenuation of the aging phenotypes. The incidence of apoptosis and cellular senescence were upregulated in p21-TKO mice tissue and downregulated in p16-TKO mice. Surprisingly, cellular proliferation in p21-TKO mice tissue was also upregulated, and the p21-TKO mice did not show telomere shortening compared with age-matched DKO mice, although p16-TKO mice displayed obvious enhancement of telomere lengthening. Consistent with these phenotypes, the SIRT1-PGC1 pathway was upregulated in p16-TKO but downregulated in p21-TKO compared with DKO mouse embryo fibroblasts (MEFs). However, the DNA damage response pathway was highly activated in p21-TKO, but rescued in p16-TKO, compared with DKO MEFs. These data suggest that p21 protected the stem cell reservoir by regulating cellular proliferation and turnover at a proper rate and that p21 loss in WS activated fairly severe DNA damage responses (DDR), which might cause an abnormal increase in tissue homeostasis. On the other hand, p16 promoted cellular senescence by inhibiting cellular proliferation, and p16 deficiency released this barrier signal without causing severe DDR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2021.597566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901894PMC
February 2021

LncRNA SNHG5 promotes cervical cancer progression by regulating the miR-132/SOX4 pathway.

Autoimmunity 2021 03 23;54(2):88-96. Epub 2021 Feb 23.

Department of Laboratory, Hangzhou Jianggan District People's Hospital, Hangzhou, China.

Background: The long non-coding RNA (lncRNA) small nucleolar RNA host gene 5 (SNHG5) has been verified as a crucial regulator in many types of tumours but not clear in cervical cancer (CC). This study aims to investigate the effect and further mechanisms of lncRNA SNHG5 in CC.

Methods: The expression of SNHG5 and miR-132, as well as SOX4 (sex-determining region Y-box 4) mRNA expression were determined by quantitative real-time PCR (qRT-PCR). The protein level of SOX4 and epithelial-mesenchymal transition (EMT)-related proteins were evaluated by western blot. Then, Edu and Transwell assay were performed to assess the proliferation, migration and invasion of CC cells. RNA immunoprecipitation (RIP) and RNA pull-down assay were conducted to explore the relationship between SNHG5 and miR-132.

Results: SNHG5 and SOX4 were upregulated, and miR-132 was downregulated in CC tissues and cell lines. SNHG5 was positively correlated with FIGO stage ( = .003) and lymph node metastasis ( = .001). Pearson's correlation analysis conveyed that SNHG5 was positively correlated with SOX4, and miR-132 was negatively correlated with SOX4 and SNHG5. Knockdown of SNHG5 reduced CC cell proliferation, migration and invasion through regulating miR-132. Moreover, overexpression of miR-132 restrained CC cell proliferation, migration, and invasion through targeting SOX4, and SNHG5 enhanced SOX4 expression negatively regulating miR-132.

Conclusion: SNHG5 promotes SOX4 expression to accelerate CC cell proliferation, migration and invasion through negatively regulating miR-132.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/08916934.2020.1864731DOI Listing
March 2021

Mechanisms and regulation of IL-22-mediated intestinal epithelial homeostasis and repair.

Life Sci 2021 Apr 10;271:119195. Epub 2021 Feb 10.

AbbVie Bioresearch Center, 100 Research Drive, Worcester, MA 01605, USA. Electronic address:

Aims: Ulcerative colitis and Crohn's disease, collectively known as inflammatory bowel disease (IBD), are chronic inflammatory disorders of the intestine for which key elements in disease initiation and perpetuation are defects in epithelial barrier integrity. Achieving mucosal healing is essential to ameliorate disease outcome and so new therapies leading to epithelial homeostasis and repair are under investigation. This study was designed to determine the mechanisms by which IL-22 regulates intestinal epithelial cell function.

Main Methods: Human intestinal organoids and resections, as well as mice were used to evaluate the effect of IL-22 on stem cell expansion, proliferation and expression of mucus components. IL-22 effect on barrier function was assessed in polarized T-84 cell monolayers. Butyrate co-treatments and organoid co-cultures with immune cells were performed to monitor the impact of microbial-derived metabolites and inflammatory environments on IL-22 responses.

Key Findings: IL-22 led to epithelial stem cell expansion, proliferation, barrier dysfunction and anti-microbial peptide production in human and mouse models evaluated. IL-22 also altered the mucus layer by inducing an increase in membrane mucus but a decrease in secreted mucus and goblet cell content. IL-22 had the same effect on anti-microbial peptides and membrane mucus in both healthy and IBD human samples. In contrast, this IL-22-associated epithelial phenotype was different when treatments were performed in presence of butyrate and organoids co-cultured with immune cells.

Significance: Our data indicate that IL-22 promotes epithelial regeneration, innate defense and membrane mucus production, strongly supporting the potential clinical utility of IL-22 as a mucosal healing therapy in IBD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119195DOI Listing
April 2021

ILF3-AS1 promotes cell proliferation and inhibits cell apoptosis of breast cancer by binding with miR-4429 to upregulate RAB14.

Hum Exp Toxicol 2021 Jul 2;40(7):1183-1193. Epub 2021 Feb 2.

Department of Medical Imaging, 26488Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning, China.

As one of the leading causes of cancer-related deaths among women, breast cancer accounts for a 30% increase of incidence worldwide since 1970s. Recently, increasing studies have revealed that the long non-coding RNA ILF3-AS1 is involved in the progression of various cancers. Nevertheless, the role of ILF3-AS1 in breast cancer remains largely unknown. In the present study, we found that ILF3-AS1 was highly expressed in breast cancer tissues and cells. ILF3-AS1 silencing inhibited breast cancer cell proliferation, migration and invasion, and promoted cell apoptosis. ILF3-AS1 bound with miR-4429 in breast cancer cells. Moreover, RAB14 was a downstream target of miR-4429, and miR-4429 expression was negatively correlated with RAB14 or ILF3-AS1 expression in breast cancer tissues. The result of rescue experiments demonstrated that overexpression of RAB14 can reverse the inhibitory effect of ILF3-AS1 knockdown on breast cancer cell proliferation, migration and invasion. Overall, ILF3-AS1 promotes the malignant phenotypes of breast cancer cells by interacting with miR-4429 to regulate RAB14, which might offer a new insight into the underlying mechanism of breast cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0960327121989422DOI Listing
July 2021

The Functional Impact of Alternative Splicing on the Survival Prognosis of Triple-Negative Breast Cancer.

Front Genet 2020 14;11:604262. Epub 2021 Jan 14.

School of Life Sciences and Technology, Xidian University, Xi'an, China.

Purpose: Triple-negative breast cancer (TNBC) is a type of breast cancer (BC) showing a high recurrence ratio and a low survival probability, which requires novel actionable molecular targets. The involvement of alternative splicing (AS) in TNBC promoted us to study the potential roles of AS events in the survival prognosis of TNBC patients.

Methods: A total of 150 TNBC patients from The Cancer Genome Atlas (TCGA) were involved in this work. To study the effects of AS in the recurrence-free survival (RFS) prognosis of TNBC, we performed the analyses as follows. First, univariate Cox regression model was applied to identify RFS-related AS events. Their host genes were analyzed by Metascape to discover the potential functions and involved pathways. Next, least absolute shrinkage and selection operator (LASSO) method was used to select the most informative RFS-related AS events to constitute an AS risk factor for RFS prognosis, which was evaluated by Kaplan-Meier (KM) and receiver operating characteristic (ROC) curves in all the data and also in different clinical subgroups. Furthermore, we analyzed the relationships between splicing factors (SFs) and these RFS-related AS events to seek the possibility that SFs regulated AS events to influence RFS. Then, we evaluated the potential of these RFS-related AS events in the overall survival (OS) prognosis from all the above aspects.

Results: We identified a total of 546 RFS-related AS events, which were enriched in some splicing and TNBC-associated pathways. Among them, seven RFS-related events were integrated into a risk factor, exhibiting satisfactory RFS prognosis alone and even better performance when combined with clinical tumor-node-metastasis stages. Furthermore, the correlation analysis between SFs and the seven AS events revealed the hypotheses that SRPK3 might upregulate PCYT2_44231_AA to have an effect on RFS prognosis and that three other SFs may work together to downregulate FLAD1_7874_RI to influence RFS prognosis. In addition, the seven RFS-related AS events were validated to be promising in the OS prognosis of TNBC as well.

Conclusion: The abnormal AS events regulated by SFs may act as a kind of biomarker for the survival prognosis of TNBC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fgene.2020.604262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841428PMC
January 2021

Characteristics of the vaginal microbiomes in prepubertal girls with and without vulvovaginitis.

Eur J Clin Microbiol Infect Dis 2021 Jun 16;40(6):1253-1261. Epub 2021 Jan 16.

Department of Pediatric Gynecology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.

The present study focused on the characteristics of the vaginal microbiomes in prepubertal girls with and without vulvovaginitis. We collected 24 vaginal samples and 16 fecal samples from 10 girls aged 3-9 years with vulvovaginitis and 16 healthy girls of the same age. The samples were divided into three groups: fecal swabs from healthy controls (HF), vaginal swabs from healthy controls (HVS), and vaginal swabs from girls with vulvovaginitis (VVS). Sequencing of the V3-V4 region of the 16S rDNA gene was performed with the NovaSeq PE250 platform to reveal the vaginal microbial community structure in healthy prepubertal girls and vulvovaginitis-associated microbiota. The intestinal microbiomes of healthy children were also analyzed for comparison. This study revealed that the healthy vaginal tract in prepubertal girls was dominated by Prevotella, Porphyromonas, Ezakiella, and Peptoniphilus species, with a high diversity of microbiota. The vulvovaginitis-associated microbiota were dominated by Streptococcus, Prevotella, Haemophilus, and Granulicatella, with lower diversity than that in healthy girls. Furthermore, the compositions of the vaginal and intestinal microbiomes were completely different. ANOSIM, MRPP, Adonis, and AMOVA were used to analyze the beta diversity, and the results showed that there were significant differences in the microbial communities among the three groups. Lactobacillus deficiency and high bacterial diversity were characteristics of the vaginal microbiome in healthy prepubertal girls; this is inconsistent with that in reproductive-age women. The vulvovaginitis-associated vaginal microbiota differed dramatically from normal microbiota, and the main causative agents were not fecal in origin.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10096-021-04152-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139898PMC
June 2021

A comparative study of the disease burden attributable to PM in China, Japan and South Korea from 1990 to 2017.

Ecotoxicol Environ Saf 2021 Feb 4;209:111856. Epub 2021 Jan 4.

Department of Environmental Health, Boston University School of Public Health, Boston, MA, USA. Electronic address:

Background: Exposure to fine particulate matter (PM) is one of the leading contributors to disease burden. However, little is known about the spatial and temporal trends of the disease burden attributable to PM in the three major economies in East Asia. We aimed to estimate the patterns and temporal variations of the disease burden attributable to PM in China, Japan, and South Korea from 1990 to 2017.

Methods: We obtained data on disease burden attributable to PM from the Global Burden of Disease Study (GBD) 2017. We retrieved the numbers and age-standardized mortality rate (ASMR) and disability-adjusted life years (DALYs) rate (ASDR) of disease attributable to PM by age, sex, socio-demographic index (SDI), and country. We used percentage change and estimated annual percentage change (EAPC) to assess the trends of ASMR and ASDR attributable to PM between 1990 and 2017. We further calculated the contribution of population growth, population aging, and changes in mortality or DALYs rate to the net changes in total deaths and DALYs associated with PM.

Results: We found considerable differences in the disease burden attributable to PM in China, Japan, and South Korea. In 2017, the ASMR and ASDR of disease attributable to PM in China were 49.37 (95% UI: 41.18, 57.5) per 100,000 population and 1065.9 (95% UI: 891.28, 1237.38) per 100,000 population, respectively, which was about four times higher than that of Japan and twice higher than that of South Korea. Regardless of country, the ASMR and ASDR were more pronounced among elders and males. From 1990 to 2017, the declines in ASMR and ASDR were more pronounced in Japan and South Korea than in China. The changes in PM associated total deaths and DALYs between 1990 and 2017 were the combined effects of population aging, population growth, and changes in mortality or DALY rate, resulting in a net increase in total deaths and DALYs in China but little changes in Japan and South Korea.

Conclusions: PM still contributed to significant disease burdens in 2017, although age-standardized disease burden has declined from 1990 to 2017. There has been an increasing trend in total deaths and DALYs in China, which was primarily driven by population aging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2020.111856DOI Listing
February 2021

Comprehensive RNA expression profile of therapeutic adipose‑derived mesenchymal stem cells co‑cultured with degenerative nucleus pulposus cells.

Mol Med Rep 2021 03 5;23(3). Epub 2021 Jan 5.

Trauma Centre, Department of Trauma and Orthopedics, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.

Stem cell‑based therapy is a promising alternative to conventional approaches to treating intervertebral disc degeneration (IDD). However, comprehensive understanding of stem cell‑based therapy at the gene level is still lacking. In the present study, we identified the expression profiles of messenger RNAs (mRNAs) and long non‑coding RNAs (lncRNAs) expressed within a co‑culture system of adipose‑derived mesenchymal stem cells (ASCs) and degenerative nucleus pulposus cells (NPCs) and explored the signaling pathways involved and their regulatory networks. Microarray analysis was used to compare ASCs co‑cultured with degenerative NPCs to ASCs cultured alone, and the underlying regulatory pattern, including the signaling pathways and competing endogenous RNA (ceRNA) network, was analyzed with robust bioinformatics methods. The results showed that 360 lncRNAs and 1757 mRNAs were differentially expressed by ASCs, and the microarray results were confirmed by quantitative PCR. Moreover, 589 Gene Ontology terms were upregulated, whereas 661 terms were downregulated. A total of 299 signaling pathways were significantly altered. A Path‑net and a Signal‑net were built to show interactions among differentially expressed genes. An mRNA‑lncRNA co‑expression network was constructed to reveal the interplay among differentially expressed mRNAs and lncRNAs, whereas a ceRNA network was built to investigate their connections with microRNAs involved in IDD. To the best of our knowledge, this original and comprehensive exploration reveals differentially expressed lncRNAs and mRNAs of ASCs stimulated by degenerative NPCs, underscoring the regulation pattern within the co‑culture system at the gene level. These data may further understanding of NPC‑directed differentiation of ASCs and facilitate the application of ASCs in future treatments for IDD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2021.11824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809910PMC
March 2021

Global burden of ischemic heart disease attributable to ambient PM pollution from 1990 to 2017.

Chemosphere 2021 Jan 27;263:128134. Epub 2020 Aug 27.

Department of Environmental Health, Boston University School of Public Health, Boston, MA, USA. Electronic address:

Objectives: We aimed to estimate the spatial and temporal variation in the PM associated ischemic heart disease (IHD) burden on a global scale between 1990 and 2017.

Methods: We obtained data on IHD attributable to PM from the Global Burden of Disease Study (GBD) 2017. We used the numbers and age-standardized mortality rate (ASMR) and disability-adjusted life years (DALYs) rate (ASDR) of IHD attributable to PM by sex, socio-demographic index (SDI), and countries. We calculated the estimated annual percentage changes (EAPCs) to assess the trends of ASMR and ASDR between 1990 and 2017. We further calculated the contribution of population growth, population aging, and mortality or DALYs changes to the total IHD deaths and DALYs attributable to PM between 1990 and 2017.

Results: In 2017, IHD attributable to PM resulted in 977,140 (95% UI: 838,900-1123,240) deaths and 21.93 million (95% UI: 18.88-25.37) DALYs globally. There has been a significant change of attributable IHD burden, from being a common burden to one that mainly affects low and middle-SDI countries in Asia, Oceania and sub-Saharan Africa. This global change has occurred as a consequence of opposing trends in high-SDI countries and in Asia, Oceania and sub-Saharan Africa, which has led to some Asian countries having the highest IHD burden attributable to PM in 2017.

Conclusions: Although the global age-standardized burden of IHD attributable to PM has decreased from 1990 to 2017, there has been an unpleasant increase in some low and middle-income countries, mainly in Asia, Oceania, and Africa.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.128134DOI Listing
January 2021

Reduced Surgical Time and Higher Accuracy of Distal Locking with the Electromagnetic Targeting System in Humeral Shaft Intramedullary Nailing.

Orthop Surg 2020 Oct 6;12(5):1413-1420. Epub 2020 Sep 6.

Department of Orthopaedic Traumatology, Trauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective: To evaluate the efficacy of the electromagnetic distal targeting system in the treatment of humeral shaft fractures.

Methods: From January 2012 to December 2018, we retrospectively reviewed 60 patients with humeral shaft fractures treated by intramedullary nailing. Among 60 cases, 41 were men and 19 were women. The average age at surgery was 48.5 years (range, 21-81 years). We performed the same surgical procedure for all patients. According to the different distal locking technique used, all patients were divided into two groups. The standard fluoroscopic freehand technique was used for Group FH, while the electromagnetic real-time targeting technique was used for Group EM. All procedures were performed by two senior surgeons. Overall surgical time, cases of complications, failure of distal locking, union time, and shoulder function assessment were recorded intraoperatively and during follow-up.

Results: Twenty-seven patients in Group FH and 33 patients in Group EM met the inclusion criteria. No significant difference was found in the demographic data of the two groups. The mean surgical time was 76.48 ± 10.73 min in Group FH and 65.61 ± 8.91 min in Group EM (P < 0.05), showing significant difference. Seven failures occurred in Group FH and two failures occurred in Group EM (P < 0.05). No relevant complications were noted. The average union time was 3.37 ± 0.49 months in Group FH and 3.39 ± 0.50 months in Group EM (P = 0.855). The mean follow-up was 14.30 ± 2.28 months in Group FH and 15.27 ± 2.83 months in Group EM (P = 0.153). The disabilities of the arm, shoulder and hand score (DASH) score, the range of motion (checked with the constant score), and the degree of functionality were, respectively, 21.52 ± 3.23, 27.04 ± 1.84, and 81.31% ± 3.88% in Group FH and 19.09 ± 2.40, 26.18 ± 1.70, and 77.97% ± 3.91% in Group EM (P = 0.233, 0.971, and 0.607).

Conclusion: The electromagnetic real-time targeting system reduced surgical time and improved accuracy, and there was no radiation exposure in the distal locking procedure for humeral shaft fractures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/os.12785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7670153PMC
October 2020

Identification of Hub Genes Associated with Hypertension and Their Interaction with miRNA Based on Weighted Gene Coexpression Network Analysis (WGCNA) Analysis.

Med Sci Monit 2020 Sep 5;26:e923514. Epub 2020 Sep 5.

Urban Construction Vocational College, Shanghai, China (mainland).

BACKGROUND Hypertension is one of the most widespread health conditions in the world, and the molecular mechanism of it is still unclear. In this study, we identified the hub genes (hub miRNA genes) associated with hypertension and explored the relationship between hypertension miRNA-gene by constructing a mRNA co-expression network and a miRNA co-expression network, which can help to reveal the mechanism and predict the prognosis of hypertension progression. MATERIAL AND METHODS Based on gene expression profile data of hypertensive samples from the Gene Expression Omnibus database, WGCNA was used to detect hypertension-related biomarkers and key mRNA and miRNA modules. Then, DAVID was used to perform gene-annotation enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) and miRPath were used for pathway analysis of mRNA and miRNAs genes. RESULTS We identified 3 key modules relating to hypertension, 2 mRNA modules named Msaddlebrown and Mgreenyellow and 1 miRNA module named Msalmon. In addition, 12 hub genes (RPL21, RPS28, LOC442727/PTGAP10, LOC100129599/RPS29P14, TBXAS1, FCER1G, CFP, FURIN, PECAM1, IGSF6, NCF1C, and LOC285296/UNC93B3) and 7 hub miRNAs (hsa-miR-1268a/b, hsa-miR-513c-3p, hsa-miR-4799-5p, hsa-miR-296-3p, hsa-miR-5195-5p, hsa-miR-219-2-3p, and hsa-miR-548d-5p) relating to hypertension were identified. HIF-1 signaling pathway and insulin signaling pathway were closely related to the 3 key modules. We also discovered 4 miRNAs (hsa-miR-548am-3p, hsa-miR-513c-3p, hsa-miR-182-5p, and hsa-miR-548d-5p) and 6 genes (IGF1R, GSK3B, FOXO1, PRKAR2B, HIF1A, and PIK3R1) were the core nodes in the hypertension-related miRNA-gene network, and hsa-miR-548am-3p was at the center of the network. CONCLUSIONS These findings will help improve the understanding of the pathogenesis of hypertension, and the discovered genes can serve as signatures for early diagnosis of hypertension.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.923514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491244PMC
September 2020

Proteomic analysis of aqueous humor from cataract patients with retinitis pigmentosa.

J Cell Physiol 2021 04 1;236(4):2659-2668. Epub 2020 Sep 1.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University & Shandong Academy of Medical Sciences, Qingdao, China.

A postcataract surgery complication in patients with retinitis pigmentosa (RP) is lens capsular contraction. To identify potential proteins contributing to this phenomenon, high-performance liquid chromatography/mass spectrometry-based proteomic analysis was conducted with aqueous humor samples collected from 11 patients who underwent cataract surgeries, with four patients diagnosed as RP and cataract (RP group) and the other seven with only senile cataract group. The upregulated proteins in the RP group were enriched in wound response, while downregulated proteins were enriched in cell adhesion and lens crystallins. Receptors of two dramatically upregulated proteins tenascin-C (TNC) and serotransferrin were found expressed in human lens epithelial cells (HLEs). TNC can promote primary HLEs proliferation and cell line HLE-B3 migration. This study indicates aqueous humor proteomic analysis serves as an effective way to unveil the pathogenesis of RP complications. TNC is a potential target of stimulating HLEs proliferation in RP concomitant cataract patients that worth further research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.30031DOI Listing
April 2021
-->