Publications by authors named "Xiaoming Sun"

336 Publications

Combining PET with MRI to improve predictions of progression from mild cognitive impairment to Alzheimer's disease: an exploratory radiomic analysis study.

Ann Transl Med 2022 May;10(9):513

Department of Nuclear Medicine, University Hospital Bern, Bern, Switzerland.

Background: This study aimed to explore the potential of a combination of 18F-fluorodeoxyglucose positron emission tomography (F-FDG PET) and magnetic resonance imaging (MRI) to improve predictions of conversion from mild cognitive impairment (MCI) to Alzheimer's disease (AD). The predictive performances and specific associated biomarkers of these imaging techniques used alone (single-modality imaging) and in combination (dual-modality imaging) were compared.

Methods: This study enrolled 377 patients with MCI and 94 healthy control participants from 2 medical centers. Enrolment was based on the patients' brain MRI and PET images. Radiomic analysis was performed to evaluate the predictive performance of dual-modality F-FDG PET and MRI scans. Regions of interest (ROIs) were determined using an a priori brain atlas. Radiomic features in these ROIs were extracted from the MRI and F-FDG PET scan data. These features were either concatenated or used separately to select features and construct Cox regression models for prediction in each modality. Harrell's concordance index (C-index) was then used to assess the predictive accuracies of the resulting models, and correlations between the MRI and F-FDG PET features were evaluated.

Results: The C-indices for the two test datasets were 0.77 and 0.80 for dual-modality F-FDG PET/MRI, 0.75 and 0.73 for single-modality F-FDG PET, and 0.74 and 0.76 for single-modality MRI. In addition, there was a significant correlation between the crucial image signatures of the different modalities.

Conclusions: These results indicate the value of imaging features in monitoring the progress of MCI in populations at high risk of developing AD. However, the incremental benefit of combining F-FDG PET and MRI is limited, and radiomic analysis of a single modality may yield acceptable predictive results.
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http://dx.doi.org/10.21037/atm-21-4349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347042PMC
May 2022

Risk factors for delayed intrauterine device removal among menopausal women in rural China.

Menopause 2022 Aug 2. Epub 2022 Aug 2.

Department of Family and Community Medicine, University of California, San Francisco, CA.

Objective: More women in China have received intrauterine devices (IUDs) than in the rest of the world combined. Recent reports have recognized a growing problem of delayed removal of these IUDs after menopause, especially in rural China, but few studies have examined women's knowledge and other risk factors for delayed removal.

Methods: A total of 1,014 perimenopausal and postmenopausal women aged 45 to 64 years participating in a pilot program providing free IUD removal in rural China completed a questionnaire. We examined their knowledge about when IUDs should be removed and other demographic and reproductive health characteristics and the association of these factors with whether their IUD removal was on time or delayed using bivariate contingency analysis and multivariate logistic regression.

Results: A total of 40.2% of women were having their IUDs removed more than 2 years after menopause, and 67.9% did not know the correct time for IUD removal. Women who were older, less educated, had one child, had their IUD placed in a rural facility, and with no gynecological examination in the past 2 years were less likely to know when an IUD should be removed. In multivariate analysis, knowledge, the facility where the IUD was placed, and a recent gynecological examination were independent predictors of on-time removal. At least 170 women had received a gynecological examination after menopause without the IUD being removed at that time.

Conclusions: Lack of knowledge about timing for IUD removal and structural barriers in health service organization must be addressed to improve care for millions of women with IUDs in rural China.
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http://dx.doi.org/10.1097/GME.0000000000002022DOI Listing
August 2022

Unlocking Layered Double Hydroxide as a High-Performance Cathode Material for Aqueous Zinc-Ion Batteries.

Adv Mater 2022 Jul 28:e2204320. Epub 2022 Jul 28.

State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

Advanced cathode materials play an important role in promoting aqueous battery technology for safe energy storage. Transition metal double hydroxides are usually elusive as a stable cathode for aqueous zinc-ion batteries (AZIBs) due to their unstable crystal structure, sluggish ion transportation, and insufficient active sites for zinc-ion storage. Here, a trinary layered double hydroxide (LDH) with hydrogen vacancies (Ni Mn Fe -LDH) is reported as a new cathode material for AZIBs. A reversible high capacity up to 328 mAh g can be obtained and cycle stably over 500 cycles with a capacity retention of 85%. Experimental and theoretical studies reveal that the hydrogen vacancies in LDH can expose lattice oxygen atoms as active sites for zinc-ion storage and accelerate ion diffusion by reducing the electrostatic interactions between zinc ions and the host structure. In addition, the synergy of the trinary transitional metal cations can suppress the Jahn-Teller distortion of manganese (III) oxide octahedron and enable long cycle stability. This work provides not only a series of high-performance cathode materials for AZIBs but also a novel materials design strategy that can be extended to other multi-valence metal-ion batteries.
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http://dx.doi.org/10.1002/adma.202204320DOI Listing
July 2022

Synergistic Effects in N,O-Comodified Carbon Nanotubes Boost Highly Selective Electrochemical Oxygen Reduction to H O.

Adv Sci (Weinh) 2022 Jul 28:e2201421. Epub 2022 Jul 28.

State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing, 100029, China.

Electrochemical 2-electron oxygen reduction reaction (ORR) is a promising route for renewable and on-site H O production. Oxygen-rich carbon nanotubes have been demonstrated their high selectivity (≈80%), yet tailoring the composition and structure of carbon nanotubes to further enhance the selectivity and widen working voltage range remains a challenge. Herein, combining formamide condensation coating and mild temperature calcination, a nitrogen and oxygen comodified carbon nanotubes (N,O-CNTs) electrocatalyst is synthesized, which shows excellent selective (>95%) H O selectivity in a wide voltage range (from 0 to 0.65 V versus reversible hydrogen electrode). It is significantly superior to the corresponding selectivity values of CNTs (≈50% in 0-0.65 V vs RHE) and O-CNTs (≈80% in 0.3-0.65 V vs RHE). Density functional theory calculations revealed that the C neighbouring to N is the active site. Introducing O-related species can strengthen the adsorption of intermediates *OOH, while N-doping can weaken the adsorption of in situ generated *O and optimize the *OOH adsorption energy, thus improving the 2-electron pathway. With optimized N,O-CNTs catalysts, a Janus electrode is designed by adjusting the asymmetric wettability to achieve H O productivity of 264.8 mol kg h .
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http://dx.doi.org/10.1002/advs.202201421DOI Listing
July 2022

Electrocatalytic Hydrogenation of 5-Hydroxymethylfurfural Promoted by a Ru Cu Single-Atom Alloy Catalyst.

Angew Chem Int Ed Engl 2022 Jul 25:e202209849. Epub 2022 Jul 25.

Department of Chemistry, Tsinghua University, Beijing, 100084, China.

Electrochemical reduction of biomass-derived 5-hydroxymethylfurfural (HMF) represents an elegant route toward sustainable value-added chemicals production that circumvents the use of fossil fuel and hydrogen. However, the reaction efficiency is hampered by the high voltage and low activity of electrodes (Cu, Bi, Pb). Herein, we report a Ru Cu single-atom alloy (SAA) catalyst with isolated Ru atoms on Cu nanowires that exhibits an electrochemical reduction of HMF to 2,5-dihydroxymethylfuran (DHMF) with promoted productivity (0.47 vs. 0.08 mmol cm  h ) and faradic efficiency (FE) (85.6 vs. 71.3 %) at -0.3 V (vs. RHE) compared with Cu counterpart. More importantly, the FE (87.5 %) is largely retained at high HMF concentration (100 mM). Kinetic studies by using combined electrochemical techniques suggest disparate mechanisms over Ru Cu and Cu, revealing that single-atom Ru promotes the dissociation of water to produce H* species that effectively react with HMF via an electrocatalytic hydrogenation (ECH) mechanism.
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http://dx.doi.org/10.1002/anie.202209849DOI Listing
July 2022

Biorefining process of agricultural onions to functional vinegar.

Prep Biochem Biotechnol 2022 Jul 20:1-9. Epub 2022 Jul 20.

Xinzhi College, Zhejiang Normal University, Lanxi, China.

Biorefinery of onion vinegar (OV) is attractive as a method for producing functional foods from onions or onion by-products. In this study, a two-stage fermentation of OV using ATCC9763 and CICC20001 was carried out at 28 °C, the titratable acidity reached 4.01%, and the was 69.64% at 72 h. Based on this, semi-continuous fermentation was performed, proceeded to charge-discharge consisting of three cycles, and the yield, productivity, and specific production rate were 76.71%, 17.73 g/(L·d), and 20.51 h, respectively, which was higher than fed-batch fermentation. The antioxidant experiments showed that OV significantly increased GSH-Px, SOD, and CAT enzyme activities of at 271.57, 129.26, and 314.68%, respectively. Nutritional analysis revealed that the total flavonoids and polyphenols were 3.01 mg/mL and 976.76 µg/mL, respectively. It was also shown that the acetic acid to total organic acid (A/T) ratio of OV was 79.02%, and the total free amino acid content was 262.30 mg/100 mL, 1.78-7.44 times higher than other fruit vinegar. The OV prepared in this study showed higher quality than the commercial vinegar.
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http://dx.doi.org/10.1080/10826068.2022.2098321DOI Listing
July 2022

The Practical Application of Oil Painting in the Treatment of Mental Illness.

Authors:
Xiaoming Sun

Occup Ther Int 2022 17;2022:1727507. Epub 2022 Jun 17.

Shaanxi Normal University, Shaanxi 710062, China.

With the rapid development of economy, politics, science, and technology and the improvement of living material level, people pay more attention to personal physical and mental development. The incidence of mental illness is on the rise. Psychological education curriculum learning and psychological counselling are based on communication; there are still many limitations. The art media enables patients to reveal their subconscious and potential emotional needs, so that interveners have the opportunity to explore these issues and build a bridge of communication with patients. In this paper, oil painting therapy of nonverbal communication is proposed as a way of psychological therapy. The patients in the psychological department of a hospital are served by six stages of therapy, each stage of treatment has different arrangements, and continuous service and treatment are adopted. Based on the art analysis principle of oil painting creation, the therapeutic experience obtained from oil painting creation and the experience gained from oil painting were divided into groups to compare the therapeutic effect and explore the role of oil painting art therapy. The results showed that the scores of the treatment group were significantly lower than those of the control group at 4 weeks and 6 weeks after the art therapy of oil painting creation, showing a significant downward trend overall. Oil painting art therapy may alleviate anxiety, providing data support for the application of oil painting art therapy in patients with depressive episodes.
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http://dx.doi.org/10.1155/2022/1727507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232304PMC
July 2022

A Chinese case of concurrent anti-laminin γ1 pemphigoid and anti-laminin 332-type mucous membrane pemphigoid.

J Dermatol 2022 Jul 11. Epub 2022 Jul 11.

Dermatology Hospital of Jiangxi Province, Jiangxi Provincial Clinical Research Center for Skin Diseases, Candidate Branch of National Clinical Research Center for Skin Diseases, Dermatology Institute of Jiangxi Province, The Affiliated Dermatology Hospital of Nanchang University, Nanchang, China.

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http://dx.doi.org/10.1111/1346-8138.16513DOI Listing
July 2022

Iridium Doped Pyrochlore Ruthenates for Efficient and Durable Electrocatalytic Oxygen Evolution in Acidic Media.

Small 2022 Jul 3;18(30):e2202513. Epub 2022 Jul 3.

State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

Developing highly active, durable, and cost-effective electrocatalysts for the oxygen evolution reaction (OER) is of prime importance in proton exchange membrane (PEM) water electrolysis techniques. Ru-based catalysts have high activities but always suffer from severe fading and dissolution issues, which cannot satisfy the stability demand of PEM. Herein, a series of iridium-doped yttrium ruthenates pyrochlore catalysts is developed, which exhibit better activity and much higher durability than commercial RuO , IrO , and most of the reported Ru or Ir-based OER electrocatalysts. Typically, the representative Y Ru Ir O OER catalyst demands a low overpotential of 220 mV to achieve 10 mA cm , which is much lower than that of RuO (300 mV) and IrO (350 mV). In addition, the catalyst does not show obvious performance decay or structural degradation over a 2000 h stability test. EXAFS and XPS co-prove the reduced valence state of ruthenium and iridium in pyrochlore contributes to the improved activity and stability. Density functional theory reveals that the potential-determining steps barrier of OOH* formation is greatly depressed through the synergy effect of Ir and Ru sites by balancing the d band center and oxygen intermediates binding ability.
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http://dx.doi.org/10.1002/smll.202202513DOI Listing
July 2022

Vertical "V-Y" Advancement Lifting Based on Fat Compartments: A New Approach for Lower Eyelid and Midcheek Rejuvenation.

Plast Reconstr Surg 2022 Jun 28. Epub 2022 Jun 28.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine; Shanghai, China.

Background: Lower blepharoplasty continues to improve over time. With discovery of the numerous fat compartments of the face, surgeons noticed that the appearance of the lower eyelid area through aging could be improved through midface lifting. This study introduces a vertical "V-Y" advancement lifting technique, using the concept of fat compartments for lower lid and midcheek rejuvenation.

Methods: Eighty female patients were categorized into four age groups at 20 patients each from 20- to 59-year-olds. The average measurement for the lower lid length in each group was recorded. Additionally, 318 patients underwent lower eyelid blepharoplasty combined with fat compartments advancement lifting for lid-cheek area rejuvenation from April 2015 to May 2018 were included in this study. The postoperative effect was evaluated based on pre- and postoperative photographs, lower lid length measurements, and three-dimensional facial analyses.

Results: The lower lid lengths gradually increased with age, and the greatest increase occurred in the 30- to 39-year-old group. 318 Asian female patients underwent the surgical procedure, with the patients' age ranging from 35 to 58 years old. Measurements of the lower lid length showed an average reduction of 0.31cm 6 months post-surgery. Three-dimensional facial analysis also indicated that the patient's midcheek volume increased at varying degrees post-surgery.

Conclusions: Lower eyelid blepharoplasty combined with vertical "V-Y" advancement lifting makes it possible to restore facial fat compartments. This procedure improves projection in the midcheek without any kinds of fillers, thus achieving a more youthful appearance of the lower lid and lid-cheek contour in middle-aged women.
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http://dx.doi.org/10.1097/PRS.0000000000009461DOI Listing
June 2022

An Aux/IAA Family Member, , Involved in Ethylene-Inhibited Petal Expansion in Rose ().

Genes (Basel) 2022 Jun 10;13(6). Epub 2022 Jun 10.

Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, College of Horticulture, China Agricultural University, Beijing 100193, China.

Flower size, a primary agronomic trait in breeding of ornamental plants, is largely determined by petal expansion. Generally, ethylene acts as an inhibitor of petal expansion, but its effect is restricted by unknown developmental cues. In this study, we found that the critical node of ethylene-inhibited petal expansion is between stages 1 and 2 of rose flower opening. To uncover the underlying regulatory mechanism, we carried out a comparative RNA-seq analysis. Differentially expressed genes (DEGs) involved in auxin-signaling pathways were enriched. Therefore, we identified an auxin/indole-3-acetic acid (Aux/IAA) family gene, , whose expression was development-specifically repressed by ethylene. The silencing of reduced cell expansion, resulting in diminished petal expansion and flower size. In addition, the expressions of cell-expansion-related genes, including , , and , were significantly downregulated following silencing. Our results reveal an Aux/IAA that serves as a key player in orchestrating petal expansion and ultimately contributes to flower size, which provides new insights into ethylene-modulated flower opening and the function of the Aux/IAA transcription regulator.
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http://dx.doi.org/10.3390/genes13061041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222917PMC
June 2022

Multi-roles of SO to enhance the removal of arsenic from wastewater in sulfidation processes.

J Hazard Mater 2022 08 2;436:129038. Epub 2022 May 2.

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Sulfidation has been an efficient method for arsenic (As) removal from acid wastewater, yet it is inefficient under neutral and weak acid conditions. The higher pH values resulted in the formation of the unstable As-S precipitates, especially employing NaS as the vulcanizing agent as it can increase the pH value dramatically. Here, we found that SO exhibited excellent multi-roles in As removal when applying HS-sulfidation method. The acidification effect of SO lead to the decreasing of pH values, guaranteed the stable As-S precipitates formation. Through the SO pre-treatment method, the results indicated that the pH values decreased from 7 to 2.8, with the increased HS utilization efficiencies for As(III) removal from 20.9% to 92.0%. Moreover, SO post-treatment not only increased the As(III) removal efficiency, but also eliminated the excessive sulfides in solution. The reaction mechanism analysis indicated that the liquid comproportionation reaction between SO and excessive sulfides plays a vital role. The generated nascent sulfur (N-S) can adsorb arsenic species and promote the agglomeration of As(III)-S precipitates. Furthermore, the SO and HS co-treatment exhibited excellent As(V) removal performance. This study provides a new alternative method to improve the HS-sulfidation process with SO for As removal from wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129038DOI Listing
August 2022

Effect of in ovo manganese injection on the embryonic development, antioxidation, hatchability, and performances of offspring broilers under normal and high temperatures.

Poult Sci 2022 Aug 30;101(8):101936. Epub 2022 Apr 30.

Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P. R. China. Electronic address:

Two experiments were carried out to study the effect of in ovo manganese (Mn) injection on the embryonic development, antioxidation, hatchability, and performances of offspring broilers under normal temperature (NT) and high temperature (HT). Experiment 1 was conducted to investigate the effect of in ovo Mn injection on the embryonic hatchability of Arbor Acres broiler breeders. On D 9 of incubation, a total of 684 fertilized eggs were randomly allocated to 6 treatments: the non-injected positive control (niPC) and treatments injected with 0 (the negative control, iNC), 6.25, 12.5, 25.0, or 50.0 μg Mn/egg as Mn sulfate. Experiment 2 was conducted to investigate the effect of in ovo Mn injection on the embryonic development, antioxidation and performance of offspring broilers under NT and HT. A total of 792 fertilized eggs were randomly allocated to 6 treatments in a 1 (niPC) + 1 (iNC) + 2 (injected Mn sources: Mn sulfate and Mn proteinate) × 2 (injected Mn levels: 12.5 and 25.0 μg/egg) factorial arrangement during the embryonic stage and D1 to 28 at NT. Then, 288 birds were allotted to 12 treatments in a 6 (the above embryonic treatments) × 2 (environmental temperatures: NT-22℃ vs HT-34℃) factorial arrangement from D 29 to 42. The results showed that Mn injection affected (P < 0.03) the hatchability and the maximum level of in ovo injected Mn was 25.0 μg Mn/egg. The Mn injection upregulated (P < 0.05) Mn-containing superoxide dismutase mRNA expression in the embryonic heart compared to the iNC. Hyperthermia decreased (P < 0.05) ADG and ADFI, breast muscle percentage, plsma alkaline phosphatase activity, and red color values of breast and thigh muscles, but increased (P < 0.05) F/G, plasma aspartate aminotransferase and lactic dehydrogenase activities, total cholesterol, uric acid and triiodothyronine contents, abdominal fat, light values of breast and thigh muscles of offspring broilers. The results suggest that in ovo Mn injection can enhance antioxidant ability in the chick embryonic heart.
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http://dx.doi.org/10.1016/j.psj.2022.101936DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192813PMC
August 2022

Use of deep learning-based radiomics to differentiate Parkinson's disease patients from normal controls: a study based on [F]FDG PET imaging.

Eur Radiol 2022 Jun 8. Epub 2022 Jun 8.

Institute of Biomedical Engineering, School of Communication and Information Engineering, Shanghai University, Shanghai, 200444, China.

Objectives: We proposed a novel deep learning-based radiomics (DLR) model to diagnose Parkinson's disease (PD) based on [F]fluorodeoxyglucose (FDG) PET images.

Methods: In this two-center study, 255 normal controls (NCs) and 103 PD patients were enrolled from Huashan Hospital, China; 26 NCs and 22 PD patients were enrolled as a separate test group from Wuxi 904 Hospital, China. The proposed DLR model consisted of a convolutional neural network-based feature encoder and a support vector machine (SVM) model-based classifier. The DLR model was trained and validated in the Huashan cohort and tested in the Wuxi cohort, and accuracy, sensitivity, specificity and receiver operator characteristic (ROC) curve graphs were used to describe the model's performance. Comparative experiments were performed based on four other models including the scale model, radiomics model, standard uptake value ratio (SUVR) model and DLR model.

Results: The DLR model demonstrated superiority in differentiating PD patients and NCs in comparison to other models, with an accuracy of 95.17% [90.35%, 98.13%] (95% confidence intervals, CI) in the Huashan cohort. Moreover, the DLR model also demonstrated greater performance in diagnosing PD early than routine methods, with an accuracy of 85.58% [78.60%, 91.57%] in the Huashan cohort.

Conclusions: We developed a DLR model based on [F]FDG PET images that showed good performance in the noninvasive, individualized prediction of PD and was superior to traditional handcrafted methods. This model has the potential to guide and facilitate clinical diagnosis and contribute to the development of precision treatment.

Key Points: The DLR method on [F]FDG PET images helps clinicians to diagnose PD and PD subgroups from normal controls. A prospective two-center study showed that the DLR method provides greater diagnostic accuracy.
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http://dx.doi.org/10.1007/s00330-022-08799-zDOI Listing
June 2022

Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing.

Nucleic Acids Res 2022 Apr 21. Epub 2022 Apr 21.

Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University, Wuhan 430072, Hubei, China.

It is important to control CRISPR/Cas9 when sufficient editing is obtained. In the current study, rational engineering of guide RNAs (gRNAs) is performed to develop small-molecule-responsive CRISPR/Cas9. For our purpose, the sequence of gRNAs are modified to introduce ligand binding sites based on the rational design of ligand-RNA pairs. Using short target sequences, we demonstrate that the engineered RNA provides an excellent scaffold for binding small molecule ligands. Although the 'stem-loop 1' variants of gRNA induced variable cleavage activity for different target sequences, all 'stem-loop 3' variants are well tolerated for CRISPR/Cas9. We further demonstrate that this specific ligand-RNA interaction can be utilized for functional control of CRISPR/Cas9 in vitro and in human cells. Moreover, chemogenetic control of gene editing in human cells transfected with all-in-one plasmids encoding Cas9 and designer gRNAs is demonstrated. The strategy may become a general approach for generating switchable RNA or DNA for controlling other biological processes.
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http://dx.doi.org/10.1093/nar/gkac255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071477PMC
April 2022

Onion Vinegar Quality Evaluation and its Alleviate Oxidative Stress Mechanism in Caenorhabditis elegans Via SKN-1.

Plant Foods Hum Nutr 2022 Jun 19;77(2):206-211. Epub 2022 Apr 19.

Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Normal University, Jinhua, 321004, China.

Recently, there has been renewed interest in biorefining of agricultural onion into functional products. In this study, onion vinegar (OV) are prepared by a two-stage semi-continuous fermentation method, and its content of total flavonoids (3.01 mg/mL) and polyphenols (976.76 μg/mL) is superior to other commercial vinegars. OV possesses a high radical scavenging activity and enhances the antioxidant enzyme activities in vivo, alleviating intracellular oxidative stress in Caenorhabditis elegans. Treated by OV, the 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH·), diammonium 2,2'-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS·) and 2-phenyl-4,4,5,5- tetramethylimidazoline-1-oxyl 3-Oxide (PTIO·) free radicals clearance rates are 88.76, 98.76 and 90.54%, respectively in vitro. Whereas the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) enzyme activities in C. elegans reach 271.57, 129.26, and 314.68%, respectively. Using RNAi and RT-PCR, it has been further confirmed that OV modulates transcription factor SKN-1, the nuclear factor erythroid 2-related factor 2 (Nrf2) homologous, in C. elegans, enhancing the resistance of C. elegans against sodium arsenite stress. Lifespan analysis reveals that 1 mL OV extends the maximum lifespan of the nematode to 26 days. Evidence is presented which shows that OV increases the lifespan of C. elegans by activating the SKN-1 signaling pathway. Overall, the OV is a well functional condiment, enhancing the value-added of onion.
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http://dx.doi.org/10.1007/s11130-022-00959-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192463PMC
June 2022

A Case of Clear Cell Hidradenoma Found During Abdominal Cyst Excision.

Cureus 2022 Mar 8;14(3):e22957. Epub 2022 Mar 8.

Dermatopathology, Quest Diagnostics, Kansas City, USA.

Clear cell hidradenoma (CCH), a rare yet benign finding, is a tumor that originates from sweat glands. It mainly presents as a slow-growing cystic nodule on the scalp or trunk. We report a case of a 60-year-old man who presented with an abdominal subcutaneous mass. This mass was excised and pathology confirmed the presence of CCH, with cytology negative for malignant cells. Although benign, CCHs cannot be confirmed as such until excision and pathologic analysis, which is the standard treatment of choice.
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http://dx.doi.org/10.7759/cureus.22957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8989250PMC
March 2022

Upconversion Luminescence-Initiated and GSH-Responsive Self-Driven DNA Motor for Automatic Operation in Living Cells and In Vivo.

Anal Chem 2022 04 24;94(13):5450-5459. Epub 2022 Mar 24.

Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, People's Republic of China.

In light of the worthy design flexibility and the good signal amplification capacity, the recently developed DNA motor (especially the DNA walker)-based fluorescent biosensors can offer an admirable choice for realizing bioimaging. However, this attractive biosensing strategy not only has the disadvantage of uncontrollable initiation but also usually demands the supplement of exogenous driving forces. To handle the above obstacles, some rewarding solutions are proposed here. First, on the surface of an 808 nm near-infrared light-excited low-heat upconversion nanoparticle, a special ultraviolet upconversion luminescence-initiated three-dimensional (3D) walking behavior is performed by embedding a photocleavage linker into the sensing elements, and such light-controlled target recognition can perfectly overcome the pre-triggering of the biosensor during the biological delivery to significantly boost the sensing precision. After that, a peculiar self-driven walking pattern is constructed by employing MnO nanosheets as an additional nanovector to physically absorb the sensing frame, for which the reduction of the widespread glutathione in the biological medium can bring about sufficient self-supplied Mn to guarantee the walking efficiency. By selecting an underlying next-generation broad-spectrum cancer biomarker ( messenger RNA) as the model target, we obtain that the newly formed autonomous 3D DNA motor shows a commendable sensitivity (where the limit of detection is down to 0.51 pM) and even an outstanding specificity for distinguishing single-base mismatching. Beyond this sound assay performance, our sensing approach is capable of working as a powerful imaging platform for accurately operating in various living specimens such as cells and bodies, showing a favorable diagnostic ability for cancer care.
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http://dx.doi.org/10.1021/acs.analchem.2c00830DOI Listing
April 2022

SND1 Promotes Radioresistance in Cervical Cancer Cells by Targeting the DNA Damage Response.

Cancer Biother Radiopharm 2022 Mar 10. Epub 2022 Mar 10.

Key Laboratory of Immune Microenvironment and Disease, Ministry of Education, Tianjin Medical University, Tianjin, China.

Radiotherapy is one of the most effective therapeutic strategies for cervical cancer patients, although radioresistance-mediated residual and recurrent tumors are the main cause of treatment failure. However, the mechanism of tumor radioresistance is still elusive. DNA damage response pathways are key determinants of radioresistance. The purpose of this study was to investigate the role and mechanism of SND1 in radioresistance of cervical cancer. A stable HeLa cell line with SND1 knockout (HeLa-KO) was generated through a modified CRISPR/Cas9 double-nicking gene editing system. The stable CaSki cell lines with SND1 knockdown (CaSki-Ctrl, CaSki-SND1-sh-1, CaSki-SND1-sh-2) were constructed through lentivirus transfection with the pSil-SND1-sh-1 and pSil-SND1-sh-2 plasmids. It was observed that SND1 deficiency significantly increased the radiosensitivity of cervical cancer cells. It was also found that silencing SND1 promotes radiation-induced apoptosis. Significantly, the cells with a loss of SND1 function exhibited inefficient ataxia telangiectasia mutated pathway activation, subsequently impairing DNA repair and G2/M checkpoint arrest. In addition, threonine 103 is an important phosphorylation site of SND1 under DNA damaging stress. Collectively, the results of this study reveal a potent radiosensitizing effect of silencing SND1 or T103 mutation on cervical cancer cells, providing novel insights into potential therapeutic strategies for cervical cancer treatment.
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http://dx.doi.org/10.1089/cbr.2021.0371DOI Listing
March 2022

Secretory Fluid-Aggregated Janus Electrospun Short Fiber Scaffold for Wound Healing.

Small 2022 Mar 10:e2200799. Epub 2022 Mar 10.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhi Zao Ju Road, Shanghai, 200011, P. R. China.

Exudate management is critical to improve chronic wound healing. Herein, inspired by a Janus-structured lotus leaf with asymmetric wettability, a Janus electrospun short fiber scaffold is fabricated via electrospinning technologies and short fiber modeling. This scaffold is composed of hydrophilic 2D curcumin-loaded electrospun fiber and hydrophobic 3D short fiber via layer-by-layer assembly and electrostatic interactions which can aggregate the wound exudate by pumping from the hydrophobic layer to the hydrophilic via multiple contact points between hydrophilic and hydrophobic fibers, and simultaneously trigger the cascade release of curcumin in the upper 2D electrospun fiber. The 3D short fiber with high porosity and hydrophobicity can quickly aggregate exudate within 30 s after compounding with hydrophilic 2D electrospun fiber via a spontaneous pump. In vitro experiments show that Janus electrospun short fiber has good biocompatibility, and the cascade release of curcumin can significantly promote the proliferation and migration of fibroblasts. In vivo experiments show that it can trigger cascade release of curcumin by aggregating wound exudate, so as to accelerate wound healing process and promote collagen deposition and vascularization. Hence, this unique biometric Janus scaffold provides an alternative for chronic wound healing.
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http://dx.doi.org/10.1002/smll.202200799DOI Listing
March 2022

Decabromodiphenyl ether induces ROS-mediated intestinal toxicity through the Keap1-Nrf2 pathway.

J Biochem Mol Toxicol 2022 Apr 9;36(4):e22995. Epub 2022 Mar 9.

State Key Laboratory of Food Science and Technology, Institute of Nutrition and School of Food Science, Nanchang University, Nanchang, China.

Polybrominated diphenyl ethers (PBDEs) are widely used brominated flame retardants as commercial products. PBDEs have been demonstrated to induce hepatic, reproductive, neural, and thyroid toxicity effects. This study aimed to clarify the potential intestinal toxicity effects of decabrominated diphenyl ether (PBDE-209) in vivo and in vitro. First, we investigated the change of PBDE-209 on oxidative stress in the intestine of mice. Subsequently, the potential toxicity mechanism of PBDE-209 in vitro was investigated. Caco-2 cells were treated with different concentrations of PBDE-209 (1, 5, and 25 μmol/L) for 24  and 48 h. We determined the cell viability, reactive oxygen species (ROS) level, multiple cellular parameters, and relative mRNA expressions. The results showed that PBDE-209 significantly injured the colon of mice, increased the intestinal levels of malondialdehyde (MDA), and changed the antioxidant enzyme activities. PBDE-209 inhibited the proliferation and induced cytotoxicity of Caco-2 cells. The change in ROS production and mitochondrial membrane potential (MMP) revealed that PBDE-209 caused oxidative stress in Caco-2 cells. The real-time PCR assays revealed that PBDE-209 inhibited the mRNA expression level of antioxidative defense factor, nuclear factor erythroid 2-related factor 2 (Nrf2). Furthermore, the FAS and Cytochrome P450 1A1 (CYP1A1) mRNA expression levels were increased in Caco-2 cells. These results suggested that PBDE-209 exerts intestinal toxicity effects in vivo and in vitro and inhibits the antioxidative defense gene expression in Caco-2 cells. This study provides an opportunity to advance the understanding of toxicity by the persistent environmental pollutant PBDE-209 to the intestine.
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http://dx.doi.org/10.1002/jbt.22995DOI Listing
April 2022

Deciphering antibiotic resistance genes and microbial community of anammox consortia under sulfadiazine and chlortetracycline stress.

Ecotoxicol Environ Saf 2022 Mar 5;234:113343. Epub 2022 Mar 5.

Department of General Surgery, Suzhou Wuzhong People's Hospital, Suzhou 215128, China. Electronic address:

The responses of anammox consortia to typical antibiotics sulfadiazine (SDZ) and chlortetracycline (CTC) were evaluated on the aspects of general performance, microbial activity, diversity and abundance of antibiotic resistance genes (ARGs), and microbial host of ARGs in anammox system. Results showed the anammox consortia had a stable performance and great resistance to 10 mg/L of SDZ, while 1 mg/L of CTC induced an unrecoverable inhibitory influence on nitrogen removal performance and anammox activity without any special treatment. The absolute abundances of anammox functional genes (nirS, hzsA and hdh) were stimulated by the acclimation to SDZ stress, however, they were much lower than the initial levels under CTC stress. In anammox consortia, ARGs comprised 18 types (94 subtypes) derived from over 20 genera. Strikingly, the anammox bacteria (AnAOB) "Ca. Brocadia" occupied 46.81% of the SDZ resistance genes (sul1 and sul2) and 38.63% of CTC resistance genes (tetX, tetG and rpsJ), and thus were identified as the dominant antibiotic resistance bacteria (ARB). Therefore, harboring the corresponding ARGs by AnAOB could be the primary protective mechanism to interpret the resistance of anammox consortia to antibiotics stress. Meanwhile, co-occurring of ARGs in anammox consortia suggested the synergistic cooperation of different ARGs could be an essential strategy to alleviate the SDZ and CTC stress. The present study proposed a new interpretation of possible mechanism that cause antibiotic resistance of anammox consortia.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113343DOI Listing
March 2022

Spatial distribution and migration of Pu in Chinese soils.

Sci Total Environ 2022 Jun 12;824:153724. Epub 2022 Feb 12.

School of Natural Resource and Surveying, Nanning Normal University, Nanning 530001, PR China.

The migration of radionuclides is a critical threat to the soil and groundwater environment. This study investigates highly radiological toxic Pu in 647 surface soils and 66 soil cores in China. First, the spatial distributions of Pu activities and Pu/Pu ratios are presented in Chinese surface soils. Second, four different types of vertical distribution of Pu, namely 45.4%, 40.9%, 9.1% and 4.5% of Chinese soil cores proportions are integrated using statistical analysis. Furthermore, different soil types are accompanied by different Pu vertical distributions, which relate closely to the Pu migration. Finally, based on the Convection Dispersion Equation (CDE) model, the present work find that the apparent convection velocities of Pu are ranging from 0.00032 ± 0.00031 cm a to 0.473 ± 0.083 cm a. As shown by the four typical vertical activity distribution of Pu in soil cores, the deepened activity maximum value position of Pu implies a fast migration rate or apparent convection velocity. This study, for the first time, suggests a significant linear correlation (R = 0.847) between the depth of Pu maximum value (cm) and the apparent convection velocity (v, cm a). We hypothesize that v usually does not exceed 0.5 cm a in the CDE model. A significant linear correlation is also identified between apparent dispersion coefficient (D, cm a) and v in Chinese soil cores from the CDE model. It will provide an important reference for harmful heavy metal migration research in the future.
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http://dx.doi.org/10.1016/j.scitotenv.2022.153724DOI Listing
June 2022

LINC00943 acts as miR-338-3p sponge to promote MPP-induced SK-N-SH cell injury by directly targeting SP1 in Parkinson's disease.

Brain Res 2022 05 3;1782:147814. Epub 2022 Feb 3.

Vice President of The Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University, Suzhou, Jiangsu, China. Electronic address:

Background: Abnormal expression of long non-coding RNA (lncRNA) is associated with the progression of Parkinson's disease (PD). LINC00943 has been proved to play an important role in the development of PD, so its role and mechanism in PD progression are worth further exploration.

Methods: MPTP was used to construct PD mice model, and its active ingredient MPP was used to construct PD cell model. Cell proliferation and apoptosis were determined by MTT assay, EdU staining and flow cytometry. The protein levels of Cyclin D1, Bcl-2 and specificity protein 1 (SP1) were tested by western blot analysis. The concentrations of inflammation factors were examined by ELISA assay. The expression levels of LINC00943, microRNA (miR)-338-3p and SP1 were measured using quantitative real-time PCR. The interaction between miR-338-3p and LINC00943 or SP1 was confirmed using dual-luciferase reporter assay and RIP assay.

Results: Our data showed that LINC00943 was highly expressed in the brain tissues of MPTP-treated mice and MPP-induced SK-N-SH cells. Knockdown of LINC00943 could promote the proliferation, while inhibit the apoptosis and inflammation of MPP-induced SK-N-SH cells to alleviate cell injury. In terms of mechanism, we pointed out that LINC00943 could sponge miR-338-3p, and miR-338-3p could target SP1. The negative regulation of si-LINC00943 on MPP-induced SK-N-SH cell injury could be reversed by miR-338-3p inhibitor. Moreover, miR-338-3p had a protective effect on SK-N-SH cells from MPP-induced injury, which could be reversed by SP1 overexpression. Additionally, we confirmed that LINC00943 positively regulated SP1 via sponging miR-338-3p.

Conclusion: To sum up, our data revealed that knockdown LINC00943 might alleviate PD progression through regulating the miR-338-3p/SP1 axis.
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http://dx.doi.org/10.1016/j.brainres.2022.147814DOI Listing
May 2022

Strong Metal-Support Interaction Boosts Activity, Selectivity, and Stability in Electrosynthesis of HO.

J Am Chem Soc 2022 Feb 30;144(5):2255-2263. Epub 2022 Jan 30.

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore.

Noble metals have an irreplaceable role in catalyzing electrochemical reactions. However, large overpotential and poor long-term stability still prohibit their usage in many reactions (e.g., oxygen evolution/reduction). With regard to the low natural abundance, the improvement of their overall electrocatalytic performance (activity, selectivity, and stability) was urgently necessary. Herein, strong metal-support interaction (SMSI) was modulated through an unprecedented time-dependent mechanical milling method on Pd-loaded oxygenated TiC electrocatalysts. The encapsulation of Pd surfaces with reduced TiO overlayers is precisely controlled by the mechanical milling time. This encapsulation induced a valence band restructuring and lowered the d-band center of surface Pd atoms. For hydrogen peroxide electrosynthesis through the two-electron oxygen reduction reaction (ORR), these electronic and geometric modifications resulted in optimal adsorption energies of reaction intermediates. Thus, SMSI phenomena not only enhanced electrocatalytic activity and selectivity but also created an encapsulating oxide overlayer that protected the Pd species, increasing its long-term stability. This SMSI induced by mechanical milling was also extended to other noble metal systems, showing great promise for the large-scale production of highly stable and tunable electrocatalysts.
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http://dx.doi.org/10.1021/jacs.1c12157DOI Listing
February 2022

Domestic Violence Victimization Among Men Who Have Sex with Men in China During the COVID-19 Lockdown.

J Interpers Violence 2022 Jan 19:8862605211072149. Epub 2022 Jan 19.

Department of Health, Behavior and Society, 25802Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Many countries worldwide have implemented dedicated measures, such as shelter at home, to contain the spread of the COVID-19 virus. However, those mitigation measures may have major implications for individuals living with someone abusive or controlling. Domestic violence (DV) may be one of the unintended consequences of public health measures due to increased various stressors and reduced access to support and services. There has been a lack of empirical research on DV victimization among gender and sexual minorities, a population vulnerable to interpersonal violence and its associated adverse health outcomes. This study investigates the prevalence of DV victimization among men who have sex with men (MSM) in Jiangsu Province, China, during the COVID-19 lockdown and its correlates with COVID-19-related psychosocial and health stressors. A total of 413 MSM were recruited via snowball sampling, venue-based, and internet-based sampling from four cities in Jiangsu Province. After providing informed consent, all participants completed study questionnaires, followed by HIV testing. Over a quarter of the participants (27.4%) reported DV victimization during the COVID-19 lockdown, including experience of verbal, physical, or sexual abuse. After adjusting sociodemographic factors, DV victimization was associated with various adverse experiences during the COVID-19 lockdown, including increased stress or anxiety level, increased alcohol use, and housing instability. Study findings underscore the prevalence of DV victimization among MSM during the COVID-19 pandemic in China. The results can inform public health efforts to raise awareness and address DV victimization among MSM in the low- and middle-income country context during the COVID-19 pandemic. Adequate health and social services and economic resources are also critical to address the needs of MSM experiencing DV victimization.
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http://dx.doi.org/10.1177/08862605211072149DOI Listing
January 2022

Using radiomics-based modelling to predict individual progression from mild cognitive impairment to Alzheimer's disease.

Eur J Nucl Med Mol Imaging 2022 Jun 15;49(7):2163-2173. Epub 2022 Jan 15.

Department of Nuclear Medicine, University Hospital Bern, Bern, Switzerland.

Background: Predicting the risk of disease progression from mild cognitive impairment (MCI) to Alzheimer's disease (AD) has important clinical significance. This study aimed to provide a personalized MCI-to-AD conversion prediction via radiomics-based predictive modelling (RPM) with multicenter 18F-fluorodeoxyglucose positron emission tomography (FDG PET) data.

Method: FDG PET and neuropsychological data of 884 subjects were collected from Huashan Hospital, Xuanwu Hospital, and from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. First, 34,400 radiomic features were extracted from the 80 regions of interest (ROIs) for all PET images. These features were then concatenated for feature selection, and an RPM model was constructed and validated on the ADNI dataset. In addition, we used clinical data and the routine semiquantification index (standard uptake value ratio, SUVR) to establish clinical and SUVR Cox models for further comparison. FDG images from local hospitals were used to explore RPM performance in a separate cohort of individuals with healthy controls and different cognitive levels (a complete AD continuum). Finally, correlation analysis was conducted between the radiomic biomarkers and neuropsychological assessments.

Results: The experimental results showed that the predictive performance of the RPM Cox model was better than that of other Cox models. In the validation dataset, Harrell's consistency coefficient of the RPM model was 0.703 ± 0.002, while those of the clinical and SUVR models were 0.632 ± 0.006 and 0.683 ± 0.009, respectively. Moreover, most crucial imaging biomarkers were significantly different at different cognitive stages and significantly correlated with cognitive disease severity.

Conclusion: The preliminary results demonstrated that the developed RPM approach has the potential to monitor progression in high-risk populations with AD.
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http://dx.doi.org/10.1007/s00259-022-05687-yDOI Listing
June 2022

Parallel analysis of transcription, integration, and sequence of single HIV-1 proviruses.

Cell 2022 01 12;185(2):266-282.e15. Epub 2022 Jan 12.

Infectious Disease Division, Brigham and Women's Hospital, Boston, MA 02115, USA; Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA. Electronic address:

HIV-1-infected cells that persist despite antiretroviral therapy (ART) are frequently considered "transcriptionally silent," but active viral gene expression may occur in some cells, challenging the concept of viral latency. Applying an assay for profiling the transcriptional activity and the chromosomal locations of individual proviruses, we describe a global genomic and epigenetic map of transcriptionally active and silent proviral species and evaluate their longitudinal evolution in persons receiving suppressive ART. Using genome-wide epigenetic reference data, we show that proviral transcriptional activity is associated with activating epigenetic chromatin features in linear proximity of integration sites and in their inter- and intrachromosomal contact regions. Transcriptionally active proviruses were actively selected against during prolonged ART; however, this pattern was violated by large clones of virally infected cells that may outcompete negative selection forces through elevated intrinsic proliferative activity. Our results suggest that transcriptionally active proviruses are dynamically evolving under selection pressure by host factors.
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http://dx.doi.org/10.1016/j.cell.2021.12.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8809251PMC
January 2022

Signatures of immune selection in intact and defective proviruses distinguish HIV-1 elite controllers.

Sci Transl Med 2021 12 15;13(624):eabl4097. Epub 2021 Dec 15.

Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.

Increasing evidence suggests that durable drug-free control of HIV-1 replication is enabled by effective cellular immune responses that may induce an attenuated viral reservoir configuration with a weaker ability to drive viral rebound. Here, we comprehensively tracked effects of antiviral immune responses on intact and defective proviral sequences from elite controllers (ECs), analyzing both classical escape mutations and HIV-1 chromosomal integration sites as biomarkers of antiviral immune selection pressure. We observed that, within ECs, defective proviruses were commonly located in permissive genic euchromatin positions, which represented an apparent contrast to autologous intact proviruses that were frequently located in heterochromatin regions; this suggests differential immune selection pressure on intact versus defective proviruses in ECs. In comparison to individuals receiving antiretroviral therapy, intact and defective proviruses from ECs showed reduced frequencies of escape mutations in cytotoxic T cell epitopes and antibody contact regions, possibly due to the small and poorly inducible reservoir that may be insufficient to drive effective viral escape in ECs. About 15% of ECs harbored deletions in intact proviruses, consistent with increased viral vulnerability to host immunity in the setting of dysfunction. Together, these results suggest a distinct signature of immune footprints in proviral sequences from ECs.
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http://dx.doi.org/10.1126/scitranslmed.abl4097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202005PMC
December 2021

Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 1 to 21 d of age.

Anim Nutr 2021 Dec 14;7(4):1133-1144. Epub 2021 Sep 14.

Poultry Mineral Nutrition Laboratory, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

The current NRC dietary selenium (Se) requirement (0.15 mg/kg) of broilers is primarily based on growth performance data reported in 1986. Our study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues. A total of 384 one-d-old male broilers ( = 8 replicates/diet) were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1, 0.2, 0.3, 0.4 or 0.5 mg Se/kg in the form of NaSeO for 21 d. Regression analysis was conducted to evaluate the optimal dietary Se levels using broken-line, quadratic or asymptotic models. The activity of glutathione peroxidase (GPX) in the plasma, liver, kidney and pancreas, iodothyronine deiodinase (DIO) in the plasma, liver and pancreas, and thioredoxin reductase (Txnrd) in the liver and pancreas, the mRNA levels of , , , () , and in the liver, , , , , , and in the kidney, and , , and in the pancreas, and the protein levels of GPX4 in the liver and kidney of broilers were influenced ( < 0.05) by added Se levels, and increased quadratically ( < 0.05) with the increase of added Se levels. The estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line, quadratic or asymptotic models ( < 0.001) of the aforementioned selenoprotein expression in the plasma, liver and kidney, and 0.09 to 0.46 mg/kg based on the fitted broken-line models ( < 0.001) of the aforementioned selenoprotein expression in the pancreas. The results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma, liver and kidney, and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age.
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http://dx.doi.org/10.1016/j.aninu.2021.02.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556337PMC
December 2021
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