Publications by authors named "Xiaomin Wu"

109 Publications

Axial spreading of droplet impact on ridged superhydrophobic surfaces.

J Colloid Interface Sci 2021 Apr 19;599:130-139. Epub 2021 Apr 19.

Key Laboratory for Thermal Science and Power Engineering of Ministry of Education, Beijing Key Laboratory for CO(2) Utilization and Reduction Technology, Department of Energy and Power Engineering, Tsinghua University, Beijing 100084, China. Electronic address:

Hypothesis: Due to the complex hydrodynamics of droplet impact on ridged superhydrophobic surfaces, quantitative droplet spreading characteristics are unrevealed, limiting the practical applications of ridged superhydrophobic surfaces. During droplet impacting, the size ratio (the ratio of the ridge diameter to the droplet diameter) is an important factor that affects droplet spreading dynamics.

Experiments: We fabricated ridged superhydrophobic surfaces with size ratios ranging from zero to one, and conduct water droplet impact experiments on these surfaces at varied Weber numbers. Aided by the numerical simulations and theoretical analysis, we illustrate the droplet spreading dynamics and reveal the law on the maximum axial spreading coefficient.

Finds: The results show that the droplet spreading and retraction dynamics on ridged superhydrophobic surfaces are significantly asymmetric in the axial and spanwise directions. Focusing on the maximum axial spreading coefficient, we find it decreases first and then increases with increasing size ratios, indicating the existence of the critical size ratio. The maximum axial spreading coefficient can be reduced by 25-40% at the critical size ratio compared with that on flat surfaces. To predict the maximum axial spreading coefficient, two theoretical models are proposed respectively for size ratios smaller and larger than the critical size ratio.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2021.04.078DOI Listing
April 2021

A follow-up study shows that recovered patients with re-positive PCR test in Wuhan may not be infectious.

BMC Med 2021 03 15;19(1):77. Epub 2021 Mar 15.

State Key Laboratory of Remote Sensing Science, Center for Global Change and Public Health, College of Global Change and Earth System Science, Beijing Normal University, Beijing, China.

Background: Previous studies showed that recovered coronavirus disease 2019 (COVID-19) patients can have a subsequent positive polymerase chain reaction (PCR) test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) after they are discharged from the hospital. Understanding the epidemiological characteristics of recovered COVID-19 patients who have a re-positive test is vital for preventing a second wave of COVID-19.

Methods: This retrospective study analyzed the epidemiological and clinical features of 20,280 COVID-19 patients from multiple centers in Wuhan who had a positive PCR test between December 31, 2019, and August 4, 2020. The RT-PCR test results for 4079 individuals who had close contact with the re-positive cases were also obtained.

Results: In total, 2466 (12.16%) of the 20,280 patients had a re-positive SARS-CoV-2 PCR test after they were discharged from the hospital, and 4079 individuals had close contact with members of this patient group. All of these 4079 individuals had a negative SARS-CoV-2 PCR test.

Conclusions: This retrospective study in Wuhan analyzed the basic characteristics of recovered COVID-19 patients with re-positive PCR test and found that these cases may not be infectious.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12916-021-01954-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956402PMC
March 2021

The mitochondrial redistribution of eNOS is involved in lipopolysaccharide induced inflammasome activation during acute lung injury.

Redox Biol 2021 May 26;41:101878. Epub 2021 Jan 26.

Department of Medicine, Division of Translational & Regenerative Medicine, University of Arizona, Tucson, AZ, USA. Electronic address:

Acute lung injury (ALI) is a devastating clinical syndrome with no effective therapies. Inflammasome activation has been reported to play a critical role in the initiation and progression of ALI. The molecular mechanisms involved in regulating the activation of inflammasome in ALI remains unresolved, although increases in mitochondrial derived reactive oxygen species (mito-ROS) are involved. Our previous work has shown that the mitochondrial redistribution of uncoupled eNOS impairs mitochondrial bioenergetics and increases mito-ROS generation. Thus, the focus of our study was to determine if lipopolysaccharide (LPS)-mediated inflammasome activation involves the mitochondrial redistribution of uncoupled eNOS. Our data show that the increase in mito-ROS involved in LPS-mediated inflammasome activation is associated with the disruption of mitochondrial bioenergetics in human lung microvascular endothelial cells (HLMVEC) and the mitochondrial redistribution of eNOS. These effects are dependent on RhoA-ROCK signaling and are mediated via increased phosphorylation of eNOS at Threonine (T)-495. A derivative of the mitochondrial targeted Szeto-Schiller peptide (SSP) attached to the antioxidant Tiron (T-SSP), significantly attenuated LPS-mediated mito-ROS generation and inflammasome activation in HLMVEC. Further, T-SSP attenuated mitochondrial superoxide production in a mouse model of sepsis induced ALI. This in turn significantly reduced the inflammatory response and attenuated lung injury. Thus, our findings show that the mitochondrial redistribution of uncoupled eNOS is intimately involved in the activation of the inflammatory response in ALI and implicate attenuating mito-ROS as a therapeutic strategy in humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.redox.2021.101878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879038PMC
May 2021

WGEVIA: A Graph Level Embedding Method for Microcircuit Data.

Front Comput Neurosci 2020 6;14:603765. Epub 2021 Jan 6.

Department of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, United States.

Functional microcircuits are useful for studying interactions among neural dynamics of neighboring neurons during cognition and emotion. A functional microcircuit is a group of neurons that are spatially close, and that exhibit synchronized neural activities. For computational analysis, functional microcircuits are represented by graphs, which pose special challenges when applied as input to machine learning algorithms. Graph embedding, which involves the conversion of graph data into low dimensional vector spaces, is a general method for addressing these challenges. In this paper, we discuss limitations of conventional graph embedding methods that make them ill-suited to the study of functional microcircuits. We then develop a novel graph embedding framework, called Weighted Graph Embedding with Vertex Identity Awareness (WGEVIA), that overcomes these limitations. Additionally, we introduce a dataset, called the five vertices dataset, that helps in assessing how well graph embedding methods are suited to functional microcircuit analysis. We demonstrate the utility of WGEVIA through extensive experiments involving real and simulated microcircuit data.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fncom.2020.603765DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815934PMC
January 2021

Effects of laser debonding treatment on the optical and mechanical properties of all-ceramic restorations.

Lasers Med Sci 2021 Jan 15. Epub 2021 Jan 15.

Department of Stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China.

The objective of this study was to evaluate how erbium-doped yttrium aluminium garnet (Er:YAG) laser debonding treatment affects the optical and mechanical properties of dental ceramics. In total, 120 rectangular (22*5*1.2 mm) IPS E.max Press specimens were fabricated and divided into 4 groups: the control group, 3 W laser group, 4 W laser group, and 5 W laser group. For each group, 10 specimens were used for the colour test (colour difference (△E) and transparency parameter (TP)), 10 specimens were used for the flexural strength test, and 10 were used for the Vickers hardness test. One random sample from each colour test specimens was observed by scanning electron microscopy (SEM). The L*, a*, b*, △E, TP, flexural strength, and Vickers hardness values were measured and calculated. According to the Kruskal-Wallis test, the L*, a*, and b* values showed no significant variations (P > 0.05), except for the b* value in the 5 W laser group (P < 0.05). The △E, TP, flexural strength, and Vickers hardness values were analysed by one-way analysis of variance (ANOVA). The 5 W laser group exhibited a higher △E value, which exceeded the perceptible threshold and significantly lower TP values than the other groups (P < 0.05). The mean flexural strength and Vickers hardness values after Er:YAG laser debonding revealed no significant changes (P > 0.05). Microcracks were detected during the SEM analysis of the 5 W laser group. Er:YAG laser debonding treatment did not affect the mechanical properties, but changed the optical properties of dental ceramics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10103-020-03219-4DOI Listing
January 2021

Ultimate jumping of coalesced droplets on superhydrophobic surfaces.

J Colloid Interface Sci 2021 Apr 8;587:429-436. Epub 2020 Dec 8.

Key Laboratory for Thermal Science and Power Engineering of Ministry of Education, Department of Energy and Power Engineering, Tsinghua University, Beijing 100084, China. Electronic address:

Hypothesis: Jumping of coalesced droplets on superhydrophobic surfaces (SHSs) is widely used for enhanced condensation, anti-icing/frosting, and self-cleaning due to its superior droplet transport capability. However, because only a tiny fraction (about 5%) of the released excess surface energy during coalescence can be transformed into jumping kinetic energy, the jumping is very weak, limiting its application.

Methods: We experimentally propose enhanced jumping methods, use machine learning to design structures that achieve ultimate jumping, and finally combine experiments and simulations to investigate the mechanism of the enhanced jumping.

Finding: We find that a more orderly flow inside the droplets through the structure is the key to improve energy transfer efficiency and that the egg tray-like structure enables the droplet to jump with an energy transfer efficiency 10.6 times higher than that of jumping on flat surfaces. This energy transfer efficiency is very close to the theoretical limit, i.e., almost all the released excess surface energy is transformed into jumping kinetic energy after overcoming viscous dissipation. The ultimate jumping enhances the application of water droplet jumping and enables other low surface energy fluid such as R22, R134a, Gasoline, and Ethanol, which cannot jump on a flat surface, to jump.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2020.12.007DOI Listing
April 2021

The complete chloroplast genome sequence of (Rosaceae).

Mitochondrial DNA B Resour 2020 27;5(3):2184-2185. Epub 2020 May 27.

School of Life Sciences, Huaibei Normal University, Huaibei, China.

Cheng ex Yü, a small excellent ornamental tree species, is only distributed in Eastern China. In this study, we assembled and annotated the complete chloroplast (cp) genome of the species using the next-generation sequencing for the first time. The cp genome was 160,006 bp in size, consisting of two copies of invert repeat (IR) regions of 26,405 bp, one large single-copy (LSC) region of 87,870bp, and one small single-copy (SSC) region of 19,326 bp. The overall GC content of the genome was 36.55%. The genome was predicted to contain 128 genes, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis of 25 chloroplast genomes in Rosaceae indicated that . is most closely related to . . These findings may provide useful information to the phylogeny of the genus .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1768951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510610PMC
May 2020

Downregulation of IRAK1 Prevents the Malignant Behavior of Hepatocellular Carcinoma Cells by Blocking Activation of the MAPKs/NLRP3/IL-1β Pathway.

Onco Targets Ther 2020 14;13:12787-12796. Epub 2020 Dec 14.

Department of Oncology, People's Hospital of Huadu District, Guangzhou 510800, People's Republic of China.

Introduction: Interleukin-1 receptor-associated kinase 1 (IRAK1) was shown to contribute to a variety of cancer-related processes. However, the function of IRAK1 in hepatocellular carcinoma (HCC) pathogenesis has not been investigated in detail.

Methods: IRAK1 expression in HCC was examined by immunohistochemistry, qRT-PCR, and Western blot assays. In addition, Huh7 and Hep3B cells were transfected with IRAK1 siRNAs and/or a NOD-like receptor family pyrindomain containing 3 (NLRP3) plasmid. Western blot, EdU staining, and Transwell assays were performed to determine changes of apoptosis, proliferation, migration, and invasion in HCC cells. Moreover, changes in the expression of proteins involved in the MAPKs/NLRP3/IL-1β pathway were confirmed by Western blotting.

Results: IRAK1 was found to be highly upregulated in HCC tissues and cells. Knockdown of IRAK1 signaling prevented the proliferation, invasion, migration, epithelial-mesenchymal transition (EMT) of HCC cells. Mechanistically, we found that activation of the MAPKs/NLRP3/IL-1β pathway could be markedly suppressed by IRAK1 knockdown in HCC cells. Furthermore, our data showed that NLRP3 could partially reverse the reduced aggressive biological behaviors of HCC cells which were caused by RAK1 knockdown.

Conclusion: Knockdown of prevented HCC progression by inhibiting the ability of NLRP3 to block the MAPKs/IL-1β pathway, suggesting that approach as a strategy for treating HCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S260793DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751837PMC
December 2020

Arginine recycling in endothelial cells is regulated BY HSP90 and the ubiquitin proteasome system.

Nitric Oxide 2021 03 15;108:12-19. Epub 2020 Dec 15.

Department of Medicine, Arizona Health Sciences Center, University of Arizona, Tucson, AZ, 85721, USA. Electronic address:

Despite the saturating concentrations of intracellular l-arginine, nitric oxide (NO) production in endothelial cells (EC) can be stimulated by exogenous arginine. This phenomenon, termed the "arginine paradox" led to the discovery of an arginine recycling pathway in which l-citrulline is recycled to l-arginine by utilizing two important urea cycle enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL). Prior work has shown that ASL is present in a NO synthetic complex containing hsp90 and endothelial NO synthase (eNOS). However, it is unclear whether hsp90 forms functional complexes with ASS and ASL and if it is involved regulating their activity. Thus, elucidating the role of hsp90 in the arginine recycling pathway was the goal of this study. Our data indicate that both ASS and ASL are chaperoned by hsp90. Inhibiting hsp90 activity with geldanamycin (GA), decreased the activity of both ASS and ASL and decreased cellular l-arginine levels in bovine aortic endothelial cells (BAEC). hsp90 inhibition led to a time-dependent decrease in ASS and ASL protein, despite no changes in mRNA levels. We further linked this protein loss to a proteasome dependent degradation of ASS and ASL via the E3 ubiquitin ligase, C-terminus of Hsc70-interacting protein (CHIP) and the heat shock protein, hsp70. Transient over-expression of CHIP was sufficient to stimulate ASS and ASL degradation while the over-expression of CHIP mutant proteins identified both TPR- and U-box-domain as essential for ASS and ASL degradation. This study provides a novel insight into the molecular regulation l-arginine recycling in EC and implicates the proteasome pathway as a possible therapeutic target to stimulate NO signaling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.niox.2020.12.003DOI Listing
March 2021

Experimental and theoretical investigation of the enhancement of the photo-oxidation of Hg by CeO-modified morphology-controlled anatase TiO.

J Hazard Mater 2021 Mar 2;406:124535. Epub 2020 Dec 2.

Institute of Environmental Engineering, National Sun Yat-Sen University, No. 70, Lian-Hai Road, Kaohsiung 804, Taiwan. Electronic address:

This study aims to investigate the coeffects of predominantly exposed anatase TiO{001} and {101} and CeO loading on the photo-oxidation of Hg to relieve the adverse effects caused by higher temperatures of 50-250 °C. The effect of loading CeO on the photocatalytic activity of morphology-controlled TiO was not only investigated using DFT with U correction but also experimentally analyzed by characterizing the electrochemical properties and the formation of free radicals. The theoretical calculation showed that CeO loading on TiO{101} was more stable than that on TiO{001}. Accordingly, a larger portion of CeO was observed to anchor to the (101) plane than to the (001) plane. CeO loading is more beneficial for increasing the distribution of photo-induced electrons and holes on the surface of 7%CeTi than on the surface of TiO and increases the energy difference between the conduction band edge of 7%CeTi and the standard redox potential of O/·O. Correspondingly, the photocatalytic removal efficiencies (PREs) of Hg by 7%CeTi were significantly enhanced compared with those of pristine TiO. The effect of CeO was highly morphologically dependent on the photocatalytic activity. This study provides valuable insight into surface engineering strategies for morphology-controlled photocatalysts for air pollution control technology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2020.124535DOI Listing
March 2021

RAC1 nitration at Y IS involved in the endothelial barrier disruption associated with lipopolysaccharide-mediated acute lung injury.

Redox Biol 2021 01 13;38:101794. Epub 2020 Nov 13.

Division of Translational and Regenerative Medicine, Department of Medicine, College of Medicine Tucson, University of Arizona, Tucson, AZ, USA. Electronic address:

Acute lung injury (ALI), a devastating illness induced by systemic inflammation e.g., sepsis or local lung inflammation e.g., COVID-19 mediated severe pneumonia, has an unacceptably high mortality and has no effective therapy. ALI is associated with increased pulmonary microvascular hyperpermeability and alveolar flooding. The small Rho GTPases, RhoA and Rac1 are central regulators of vascular permeability through cytoskeleton rearrangements. RhoA and Rac1 have opposing functional outcome: RhoA induces an endothelial contractile phenotype and barrier disruption, while Rac1 stabilizes endothelial junctions and increases barrier integrity. In ALI, RhoA activity is increased while Rac1 activity is reduced. We have shown that the activation of RhoA in lipopolysaccharide (LPS)-mediated ALI, is dependent, at least in part, on a single nitration event at tyrosine (Y). Thus, the purpose of this study was to determine if the inhibition of Rac1 is also dependent on its nitration. Our data show that Rac1 inhibition by LPS is associated with its nitration that mass spectrometry identified as Y, within the switch I region adjacent to the nucleotide-binding site. Using a molecular modeling approach, we designed a nitration shielding peptide for Rac1, designated NipR2 (nitration inhibitor peptide for the Rho GTPases 2), which attenuated the LPS-induced nitration of Rac1 at Y, preserves Rac1 activity and attenuates the LPS-mediated disruption of the endothelial barrier in human lung microvascular endothelial cells (HLMVEC). Using a murine model of ALI induced by intratracheal installation of LPS we found that NipR2 successfully prevented Rac1 nitration and Rac1 inhibition, and more importantly attenuated pulmonary inflammation, reduced lung injury and prevented the loss of lung function. Together, our data identify a new post-translational mechanism of Rac1 inhibition through its nitration at Y. As NipR2 also reduces sepsis induced ALI in the mouse lung, we conclude that Rac1 nitration is a therapeutic target in ALI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.redox.2020.101794DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664366PMC
January 2021

Activation of the mechanosensitive Ca channel TRPV4 induces endothelial barrier permeability via the disruption of mitochondrial bioenergetics.

Redox Biol 2021 01 12;38:101785. Epub 2020 Nov 12.

Department of Medicine, Division of Translational & Regenerative Medicine, University of Arizona, Tucson, AZ, USA. Electronic address:

Mechanical ventilation is a life-saving intervention in critically ill patients with respiratory failure due to acute respiratory distress syndrome (ARDS), a refractory lung disease with an unacceptable high mortality rate. Paradoxically, mechanical ventilation also creates excessive mechanical stress that directly augments lung injury, a syndrome known as ventilator-induced lung injury (VILI). The specific mechanisms involved in VILI-induced pulmonary capillary leakage, a key pathologic feature of VILI are still far from resolved. The mechanoreceptor, transient receptor potential cation channel subfamily V member 4, TRPV4 plays a key role in the development of VILI through unresolved mechanism. Endothelial nitric oxide synthase (eNOS) uncoupling plays an important role in sepsis-mediated ARDS so in this study we investigated whether there is a role for eNOS uncoupling in the barrier disruption associated with TRPV4 activation during VILI. Our data indicate that the TRPV4 agonist, 4α-Phorbol 12,13-didecanoate (4αPDD) induces pulmonary arterial endothelial cell (EC) barrier disruption through the disruption of mitochondrial bioenergetics. Mechanistically, this occurs via the mitochondrial redistribution of uncoupled eNOS secondary to a PKC-dependent phosphorylation of eNOS at Threonine 495 (T495). A specific decoy peptide to prevent T495 phosphorylation reduced eNOS uncoupling and mitochondrial redistribution and preserved PAEC barrier function under 4αPDD challenge. Further, our eNOS decoy peptide was able to preserve lung vascular integrity in a mouse model of VILI. Thus, we have revealed a functional link between TRPV4 activation, PKC-dependent eNOS phosphorylation at T495, and EC barrier permeability. Reducing pT495-eNOS could be a new therapeutic approach for the prevention of VILI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.redox.2020.101785DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691184PMC
January 2021

miR-101 suppresses colon cancer cell migration through the regulation of EZH2.

Rev Esp Enferm Dig 2021 Apr;113(4):255-260

School of Life Sciences, School of Life Sciences, Information College, Huaibei Normal University, China.

Background: colorectal cancer (CRC) is one of the most prevalent types of malignancies worldwide. The incidence of CRC is steadily increasing due to extended life expectancy and aging-related genetic and epigenetic abnormalities. Dysregulation of microRNAs (miRNAs) has been implicated in CRC development.

Methods: the current study is a basic research study aimed at understanding the molecular mechanism of miR-101 in the pathogenesis of CRC using human samples in vivo and CRC cell lines in vitro. The miRNAs profile from human samples was analyzed by miRNA microarrays and the expression level of single miRNAs were confirmed by qRT-PCR. The validation of the direct target of miR-101 was performed by western blot assay. The cell mobility of CRC was assessed using the Transwell migration assay.

Results: downregulation of miR-101 was identified in 39 human CRC tissues and CRC cell lines (HT29 and SW620) when compared to their counterpart control. We further confirmed that the enhancer of zeste homolog 2 (EZH2), a histone methyltransferase, is a direct target of miR-101. Overexpression of EZH2 promoted CRC cell line migration and this effect was inhibited by forcing the expression of miR-101. Thus, we conclude that miR-101 regulated colon cancer cell migration occurs at least partially, though targeting EZH2.

Conclusion: our study suggests that miR-101 functions as a tumor suppressor in CRC, and miR-101 may be a potential therapeutic target for CRC treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.17235/reed.2020.6800/2019DOI Listing
April 2021

Controlling the Jumping Angle of Coalescing Droplets Using Surface Structures.

ACS Appl Mater Interfaces 2020 Nov 6;12(46):52221-52228. Epub 2020 Nov 6.

Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.

The jumping direction is an essential characteristic of jumping droplets, but it is poorly understood and uncontrollable at present. In this work, we present a method to control the jumping direction by surface structures, where the jumping direction is controlled by changing the inclination angle of the structure. The underlying mechanism is analyzed experimentally, with numerical simulations, and using a theoretical model developed to relate the jumping direction and the inclination angle for a few cases with a specific distribution. Because random droplet distributions are more common on actual condensation surfaces, a more comprehensive prediction model was developed based on a convolution neural network (CNN) to predict the jumping direction for more general cases. The input to the CNN is an image of droplets with various distribution features, which are detected by the neural network and used to predict the jumping angle. SHapley Additive exPlanations methods were then used to analyze the feature importance and to give human-understandable insights from the prediction model. This work offers avenues for improving cooling rates, anti-icing/freezing characteristics, and self-cleaning attributes and contributes to a better understanding of the jumping direction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c16995DOI Listing
November 2020

Mechanistic Insights to the Binding of Antibody CR3022 Against RBD from SARS-CoV and HCoV-19/SARS-CoV-2: A Computational Study.

Comb Chem High Throughput Screen 2020 10 26. Epub 2020 Oct 26.

Institute of Biomedicine, Jinan University, Guangzhou, 510632. China

Aims & Objective: Corona Virus Disease 2019 (COVID-19) caused by the human coronavirus 2019 (HCoV-19, also known as SARS-CoV-2) infection is currently in a global outbreak. COVID-19 has posed a huge threat to public health and economic stability worldwide. CR3022, a human monoclonal neutralizing antibody isolated from a Severe Acute Respiratory Syndrome (SARS) recovery patient, was confirmed to be able to bind the S protein of HCoV-19 with a certain degree of neutralizing activity. Crystal structural information indicated that CR3022 could bind to the epitope on the receptor binding domain (RBD) of HCoV-19, whose epitope consists of 28 amino acids, and 24 of them are conserved in SARS-CoV of SARS. However, the crystal structure is only a static conformation at a certain moment in time, and it cannot provide dynamic details of the interaction between antigen and antibody.

Methods: In this study, molecular dynamics (MD) simulation combined with MM/PBSA and CAS methods were performed to investigate the binding mechanism of binding of CR3022 against SARS-CoV-RBD and HCoV-19-RBD in order to determine their holographic dynamic information.

Results: It was found that the CR3022-SARS-CoV-RBD complex was more stable during 100ns MD run than that of the CR3022-HCoV19-RBD system. There were common conservative amino acids on the β2 sheet of RBD, including Tyr369, Phe377, Lys378, Tyr380, Gly381, Lys386, Leu390 and others. These conservative amino acids play significant roles in the binding process of CR3022 antibody against SARS-CoV-RBD and HCoV-19-RBD. It was also found that the binding mode of CR3022 to its native target SARS-CoV-RBD is more comprehensive and uniform. Moreover, the β2 sheet residue Thr385 and non-β2 sheet residues Arg408 and Asp428 of the CR3022- SARS-CoV-RBD system were found to be crucial for their binding affinities, thus forming a special conformational epitope. However, these key amino acids are not present in the CR3022-HCoV-19-RBD system. The binding mode of CR3022 and HCoV-19-RBD is similar to that of SARS-CoV-RBD, but the deficiency of crucial hydrogen-bonds and salt-bridges. Therefore, the binding of CR3022 and HCoV19-RBD only draws on the partial mode of the binding of CR3022 and SARS-CoV-RBD, so there is a loss of affinity.

Conclusion: Thus, in order to better fight the epidemic of COVID-19 with the CR3022 antibody, this antibody needs to further improve the neutralization efficiency of HCoV-19 through mutation of it's CDR region.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1386207323666201026160500DOI Listing
October 2020

High-Performance Organic Electrochemical Transistors with Nanoscale Channel Length and Their Application to Artificial Synapse.

ACS Appl Mater Interfaces 2020 Nov 21;12(44):49915-49925. Epub 2020 Oct 21.

Institute of Optoelectronic Display, National & Local United Engineering Lab of Flat Panel Display Technology, Fuzhou University, Fuzhou 350002, China.

Organic electrochemical transistors (OECTs) have attracted considerable interests for various applications ranging from biosensors to digital logic circuits and artificial synapses. However, the majority of reported OECTs utilize large channel length up to several or several tens of micrometers, which limits the device performance and leads to low transistor densities. Here, we demonstrate a new design of vertical OECT architecture with a nanoscale channel length down to ∼100 nm. The devices exhibit a high on-state current of over 20 mA under a low bias voltage of 0.5 V, a fast transient response of less than 300 μs, and an extraordinary transconductance up to 68.88 mS, representing a record-high value for OECTs. The excellent electrical performance is attributed to the novel structure with a nanoscale channel length defined by the channel material thickness, which is intrinsically different from that of conventional OECTs with the channel length limited by the lithography resolution. Owing to the low thermal budget, we fabricate flexible devices on a flexible substrate, which exhibit unprecedented endurance characteristics and mechanical robustness after 1000 blending cycles. Furthermore, the proposed device is capable of mimicking biological inhibitory synapses for application in intelligent artificial neural networks. Our work not only pushes the performance limit of OECTs but also opens up a new design of OECTs for high-performance biosensors, digital logic, and neuromorphic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c15553DOI Listing
November 2020

Prediction of RNA secondary structure based on stem region replacement using the RSRNA algorithm.

Comput Methods Biomech Biomed Engin 2020 Sep 9:1-14. Epub 2020 Sep 9.

College of Life Sciences, Huaibei Normal University, Huaibei, China.

RNA functions, including the regulation of various cellular activities, seem to be closely related to its structure. However, accurately predicting RNA secondary structures can be difficult. Structural prediction can be achieved by selecting stem areas that are suitable and compatible from stem pools. Here, we propose a method for predicting the secondary structure of non-coding RNA based on stem region substitution, which we named RSRNA. This method is compatible with nested RNA secondary structures, while reducing any randomness. Our algorithm had higher performance and prediction accuracy than other algorithms, which deems it more effective for future RNA structure studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/10255842.2020.1813280DOI Listing
September 2020

DSARna: RNA Secondary Structure Alignment Based on Digital Sequence Representation.

Comb Chem High Throughput Screen 2020 Aug 10. Epub 2020 Aug 10.

College of Life Science, Huaibei Normal University, Huaibei, Anhui. China.

Background: With increasing applications and development of high-throughput sequencing, knowledge of the primary structure of RNA has expanded exponentially. Moreover, the function of RNA is determined by the secondary or higher RNA structure, and similar structures are related to similar functions, such as the secondary clover structure of tRNA. Therefore, RNA structure alignment is an important subject in computational biology and bioinformatics to accurately predict function. However, the traditional RNA structure alignment algorithms have some drawbacks such as high complexity and easy loss of secondary structure information.

Objective: To study RNA secondary structure alignment according to the shortcomings of existing secondary structure alignment algorithms and the characteristics of RNA secondary structure.

Method: We propose a new digital sequence RNA structure representation algorithm named "DSARna" . Then based on a dynamic programming algorithm, the scoring matrix and binary path matrix are simultaneously constructed. The backtracking path is identified in the path matrix, and the optimal result is predicted according to the path length.

Conclusions: Upon comparison with the existing SimTree algorithm through experimental analysis, the proposed method showed higher accuracy and could ensure that the structural information is not easily lost in terms of improved specificity, sensitivity, and the Matthews correlation coefficient.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/1386207323666200811100338DOI Listing
August 2020

Dichloromethane extraction from Piper nigrum L. and P. longum L. to mitigate ischemic stroke by activating the AKT/mTOR signaling pathway to suppress autophagy.

Brain Res 2020 12 8;1749:147047. Epub 2020 Aug 8.

School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China. Electronic address:

Dichloromethane fraction (DF) of Piper nigrum L. and P. longum L. (PnL and PlL), has been found to exert a protective effect against ischemic stroke in rats. However, the regulatory mechanism exerted by PnL and PIL have not been fully elucidated. In this study, we found that DF greatly ameliorated cerebral ischemic injury in a rat model of permanent middle cerebral artery occlusion (pMCAO). The neurological score, behavioral assessment, brain infarct volume, phosphorylation of AKT (p-AKT), phosphorylation mTOR (p-mTOR), and Atg7 protein levels were determined. Additionally, we discovered that DF pretreatment reduced infarct volume, neurological score, and brain damage. Furthermore, DF therapy caused the downregulation of Atg7 and p-AKT expression, as well as the upregulation of p-mTOR expression. In conclusion, our findings indicated that DF treatment can reduce brain damage and inhibit apoptosis and autophagy by activating the Akt-mTOR signaling pathway in ischemic stroke.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.brainres.2020.147047DOI Listing
December 2020

RNF8 Promotes Epithelial-Mesenchymal Transition in Lung Cancer Cells via Stabilization of Slug.

Mol Cancer Res 2020 11 4;18(11):1638-1649. Epub 2020 Aug 4.

Department of Biology and Chemistry, College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan, China.

RNF8 (ring finger protein 8), a RING finger E3 ligase best characterized for its role in DNA repair and sperm formation via ubiquitination, has been found to promote tumor metastasis in breast cancer recently. However, whether RNF8 also plays a role in other types of cancer, especially in lung cancer, remains unknown. We show here that RNF8 expression levels are markedly increased in human lung cancer tissues and negatively correlated with the survival time of patients. Overexpression of RNF8 promotes the EMT process and migration ability of lung cancer cells, while knockdown of RNF8 demonstrates the opposite effects. In addition, overexpression of RNF8 activates the PI3K/Akt signaling pathway, knockdown of RNF8 by siRNA inhibits this activation, and pharmacologic inhibition of PI3K/Akt in RNF8-overexpressing cells also reduces the expression of EMT markers and the ability of migration. Furthermore, RNF8 is found to directly interact with Slug and promoted the K63-Ub of Slug, and knockdown of Slug disrupts RNF8-dependent EMT in A549 cells, whereas overexpression of Slug rescues RNF8-dependent MET in H1299 cells, and depletion of RNF8 expression by shRNA inhibits metastasis of lung cancer cells . Taken together, these results indicate that RNF8 is a key regulator of EMT process in lung cancer and suggest that inhibition of RNF8 could be a useful strategy for lung cancer treatment. IMPLICATIONS: This study provides a new mechanistic insight into the novel role of RNF8 and identifies RNF8 as a potential new therapeutic target for the treatment of lung cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/1541-7786.MCR-19-1211DOI Listing
November 2020

Risk of second primary malignancy in adults with pulmonary high-grade neuroendocrine carcinoma (HGNEC).

BMC Cancer 2020 Aug 3;20(1):719. Epub 2020 Aug 3.

Department of Oncology, Yancheng No.1 People's Hospital, the Affiliated Hospital of Nanjing University, 166 Yulong West Road, Yancheng, 224200, People's Republic of China.

Background: Pulmonary high-grade neuroendocrine carcinoma (HGNEC) has a rising incidence of developing second primary malignancies (SPMs). This study is the first population-based analysis to quantify the SPM risks among survivors of lung HGNEC.

Methods: We used the Surveillance, Epidemiology, and End Results (SEER) database to calculate standardized incidence ratio (SIR) and absolute excess risk (AER) between 2000 and 2016 for patients with pulmonary HGNEC.

Results: The data of 1161 patients with SPMs were retrieved from the SEER database. The ratio of observed/expected number of SPMs in pulmonary HGNEC was 1.53. Solid tumours comprised 91% of all second malignancies in lung HGNEC patients, with the most common cancers reported in the oral cavity and pharynx, the urinary and respiratory systems CONCLUSIONS: Our study observed an increased risk of SPMs among patients with pulmongnancies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-020-07224-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398247PMC
August 2020

Prognostic Value of an m6A RNA Methylation Regulator-Based Signature in Patients with Hepatocellular Carcinoma.

Biomed Res Int 2020 15;2020:2053902. Epub 2020 Jul 15.

Department of Oncology, Yancheng No. 1 People's Hospital, Nantong University, Yancheng 224200, China.

Purposes: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world. Recent researches have demonstrated that m6A methylation regulators play a key role in various cancers, such as gastric cancer and colon adenocarcinoma. Several m6A methylation regulators are reported to predict the prognosis of HCC. Therefore, there is a need to further identify the predictive value of m6A methylation regulators in HCC.

Methods: We utilized The Cancer Genome Atlas (TCGA) database to obtain the gene expression profile of m6A RNA methylation regulators and clinical information for patients with HCC. Besides, we identified two clusters of HCC with various clinical factors by consensus clustering analysis. Then the least absolute shrinkage and selection operator (LASSO) and the Cox regression analysis were applied to construct a prognostic signature.

Results: Except for ZC3H13 and METTL14, a majority of the thirteen m6A RNA methylation regulators were significantly overexpressed in HCC specimens. HCC patients were classified into two groups (cluster 1 and cluster 2). The cluster 1 was with a significantly worse prognosis than cluster 2, and most of the 13 known m6A RNA methylation regulators were upregulated in cluster 1. Besides, we developed a prognostic signature consisting of YTHDF2, YTHDF1, METTL3, KIAA1429, and ZC3H13, which could successfully differentiate high-risk patients. More importantly, univariate and multivariate Cox regression analysis indicated that the signature-based risk score was an independent prognostic factor for patients with HCC.

Conclusions: Our study showed these five m6A RNA methylation regulators can be used as practical and reliable prognostic tools of HCC, which might have potential value for therapeutic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/2053902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378627PMC
April 2021

Georgenia faecalis sp. nov. isolated from the faeces of Tibetan antelope.

J Microbiol 2020 Sep 24;58(9):734-740. Epub 2020 Jul 24.

Central & Clinical Laboratory of Sanya People's Hospital, Sanya, Hainan, 572000, P. R. China.

Two aerobic, Gram-stain-positive, non-motile, non-sporulating coccoid strains, designated ZLJ0423 and ZLJ0321, were isolated from the faeces of Tibetan antelope (Pantholops hodgsonii). Their optimal temperature, NaCl concentration and pH for growth were 28°C, 0.5% (w/v) NaCl and pH 7.5, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains ZLJ0423 and ZLJ0321 were very similar to each other (99.8%) and had a sequence similarity of 97.0% with Georgenia satyanarayanai NBRC 107612 and Georgenia subflava CGMCC 1.12782. Phylogenomic analysis based on 688 core genes indicated that these strains formed a clade with G. satyanarayanai NBRC 107612 and Georgenia wutianyii Z294. The predominant cellular fatty acids were anteiso-C, anteiso-C and C. The major menaquinone was MK-8(H). The cell-wall amino acids consisted of alanine, lysine, glycine and aspartic acid, with lysine as the diagnostic diamino acid. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unidentified lipids formed the polar lipid profile. The DNA G + C content of both isolates was 73.9 mol%. The digital DNA-DNA hybridization value between strains ZLJ0423 and ZLJ0321 was 91.2%, but their values with closely related species and other available type strains of the genus Georgenia were lower than the 70% threshold. On the basis of polyphasic taxonomic data, strains ZLJ0423 and ZLJ0321 represent a novel species within the genus Georgenia, for which the name Georgenia faecalis sp. nov. is proposed. The type strain is ZLJ0423 (= CGMCC 1.13681 = JCM 33470).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12275-020-0060-1DOI Listing
September 2020

sp. nov. and sp. nov., isolated from Tibetan antelope () and plateau pika (), respectively.

Int J Syst Evol Microbiol 2020 Aug;70(8):4683-4690

Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.

Four novel strains (592, S592, MF47 and SMF47) were isolated from Tibetan antelopes () and plateau pikas (), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to DSM 10552 (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592 and MF47 represent two novel species. The four isolates produced acid from l-rhamnose, d-xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592 and MF47 were 70.3 and 69.8 mol%, respectively. The digital DNA-DNA hybridization value between strains 592 and MF47 was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus in the NCBI database, including NBRC 14897 and JCM 14732. The major fatty acids of the four strains were Cω9 and C 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H) and MK-8(H). The cell-wall peptidoglycan contained ll-diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, sp. nov. and sp. nov. The type strains are 592 (=CGMCC1.16526=DSM 106289) and MF47 (=CGMCC 1.17444=JCM 33790), respectively.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004331DOI Listing
August 2020

High-performance Nonvolatile Organic Photoelectronic Transistor Memory Based on Bulk Heterojunction Structure.

ACS Appl Mater Interfaces 2020 Jul 29;12(28):31716-31724. Epub 2020 Jun 29.

Institute of Optoelectronic Display, National & Local United Engineering Lab of Flat Panel Display Technology, Fuzhou University, Fuzhou 350002, China.

Depending on the storage mechanisms, organic field-effect transistor (OFET) memory is usually divided into floating gate memory, ferroelectric memory, and polymer-electret-based memory. In this work, a new type of nonvolatile OFET memory is proposed by simply blending a p-type semiconductor and a n-type semiconductor without using an extra trapping layer. The results show that the memory window can be effectively modulated by the dopant concentration of the n-type semiconductor. With the addition of a 5% n-type semiconductor, blending devices exhibit a large memory window up to 57.7 V, an ON/OFF current ratio (/) ≈ 10, and a charge retention time of over 10 years, which is comparable or even better than those of most of the traditional OFET memories. The discontinuous n-type semiconductor is set as a charge-trapping center for charge storage due to the quantum well-like organic heterojunctions. The generalization of this method is also investigated in other organic systems. Moreover, the blend devices are also applied to optical memory and show multilevel optical storage, which are further scaled up to 8 × 8 array to map up two-dimensional (2D) optical images with long-term retention and reprogramming characteristic. The results reveal that the novel system design has great potential application in the field of digital image memory and photoelectronic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c09221DOI Listing
July 2020

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China.

Int J Syst Evol Microbiol 2020 Jun;70(6):3763-3774

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China.

Eight Gram-stain-positive, rod-shaped bacterial strains were isolated from faeces of Tibetan antelopes on the Tibet-Qinghai Plateau of China. Genomic sequence analysis showed that the strains belong to the genera (strains 299 and 340), (strains 2184, 2185, 2183 and 2189) and (strains 160 and 143), respectively, with a percentage of similarity for the 16S rRNA gene under the species threshold of 98.7 % except for strains 160 and 143 with CAU 1183 (98.8 %). The genome sizes (and genomic G+C contents) were 3.1 Mb (49.4 %), 2.5 Mb (64.9 %), 2.4 Mb (66.1 %) and 4.1 Mb (37.1 %) for the type strains 299, 2183, 2184 and 160, respectively. Two sets of the overall genome relatedness index values between our isolates and their corresponding closely related species were under species thresholds (95 % for average nucleotide identity, and 70 % for digital DNA-DNA hybridization). These results, together with deeper genotypic, genomic, phenotypic and biochemical analyses, indicate that these eight isolates should be classified as representing four novel species. Strain 299 (=CGMCC 1.16320=JCM 33611) is proposed as representing sp. nov.; strain 2184 (=CGMCC 1.16417=DSM 106203) is proposed as representing sp. nov.; strain 2183 (=CGMCC 1.16416=DSM 106264) is proposed as representing sp. nov.; and strain 160 (=CGMCC 1.16367=DSM 106186) is proposed as representing sp. nov.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004232DOI Listing
June 2020

sp. nov.,isolated from faeces of Tibetan gazelle ().

Int J Syst Evol Microbiol 2020 Jun;70(6):3665-3672

Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, PR China.

Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136 and dk3543, were isolated from the faeces of Tibetan gazelle () in the Qinghai-Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Ka25 (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that Ka25, XZ17, 78 and D287 were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136 and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus . ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C9, -C and C8 as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136 and dk3543 represent a novel species in genus , for which the name sp. nov. is proposed. The type strain is dk3136 (=CGMCC 4.7570=JCM 33496=KCTC 49314).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004218DOI Listing
June 2020

Directional Transportation of Impacting Droplets on Wettability-Controlled Surfaces.

Langmuir 2020 Jun 18;36(21):5855-5862. Epub 2020 May 18.

School of Aeronautic Science and Engineering, Beihang University, Beijing 100191, China.

Although a superhydrophobic surface could realize rapid rebounding (., short contact time) of an orthogonal impacting droplet, the rebounding along the original impacting route may limit its engineering application; in contrast, the directional transportation seems to be more promising. Here, we achieve directional transportation of a droplet impacting a wettability-controlled surface. When the droplet eccentrically impacts on the boundary between the superhydrophobic part and the hydrophilic part, it undergoes spreading, retracting, departure, throwing, and breaking up stages, and finally bounces off directionally. The directional transportation distance could even reach more than ten times the droplet size, considered the adhesion length (., covering length on the hydrophilic part by the droplet at the maximum spreading) is optimized. However, there is a critical adhesion length, above which the directional transportation does not occur. To be more generalized, the adhesion length is de-dimensionalized by the maximum spreading radius, and the results show that as the dimensionless adhesion length increases, the transportation distance first increases and then decreases to zero. Under the present impacting conditions, the optimal dimensionless adhesion length corresponding to the maximum transportation distance is near 0.4, and the critical dimensionless adhesion length is about 0.7. These results provide a fundamental understanding of droplet directional transportation and could be useful for related engineering applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.0c00601DOI Listing
June 2020

[Salt intake knowledge and behavior among school teachers in four Chinese cities in 2014].

Wei Sheng Yan Jiu 2020 Mar;49(2):213-219

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To learn the knowledge level on "salt and health", and analyze the influence on related behavior among school teachers in four Chinese cities.

Methods: The data of 2014 salt KAP among urban residents in China was used, with stratified random cluster sampling, a total of 2518(male 708, female 1810) school teachers were selected from four cities of China, including Shenyang, Wuhan, Chengdu and Guangzhou city. Self-filled questionnaire was used to collect the information on the salt intake knowledge and behavior. The statistical software package SAS version 9. 4 was used for data analysis.

Results: Among the teachers in four Chinese cities, about 50. 9% of them knew the recommendation of salt intake, about 66. 6% of them knew sodium in the salt may influence health. Approximately 85. 3% of them knew eating long-term salty food may cause hypertension. The proportions of knowing salt intake among female, 40 years and older, below the undergraduate, higher family income teachers were higher than their counterparts(P<0. 05). The proportions of knowing the health influence of sodium among female, higher family income teachers were higher than their counterparts(P<0. 05). The proportions of knowing that eating long-term salty food may cause hypertension among female, undergraduate and above, higher family income teachers were higher than their counterparts(P<0. 05). About 80. 1% of them knew the nutrition labeling on the package food, about 19. 7% of them knew the nutrition labeling contents. Teachers utilized nutrition labeling differently owing to their knowledge on the labeling contents(P<0. 05). In the past three months, about 21. 4% of the teachers always use low-sodium salt when cooking, only 4. 2% of them always control their family's salt intake by using salt control spoon, about 25. 4% of them always choose low-sodium food when purchasing, only 15. 1% of the teachers requested the meals cooked lightly when eating out. The proportions of teachers who used low-sodium salt among female, higher family income, knowing salt intake recommended, knowing the health influence of sodium, knowing that long-term salty food may cause hypertension, active learning related knowledge, family members suffering from hypertension were higher than their counterparts(P<0. 05). The proportions of using salt control spoon among teachers under Bachelor's degree, active learning related knowledge were higher than their counterparts(P<0. 05). The proportions of teachers purchasing low-sodium food among 40 years and older, under Bachelor's degree, higher family income, knowing salt intake recommended, knowing the health influence of sodium, knowing that long-term salty food may cause hypertension, family members not suffering from hypertension, active learning related knowledge were higher than their counterparts(P<0. 05). The proportions of teachers requesting the meals cooked slightly when eating-out among 40 years and older, under Bachelor's degree, not knowing salt intake recommended, not knowing that long-term salty food may cause hypertension, not knowing if their family members suffered from hypertension, active learning related knowledge were higher than their counterparts(P<0. 05). The teachers consumed soy sauce, bean paste, fried bread stick differently owing to their knowledge about this(P<0. 05).

Conclusion: The teachers in the four cities have grasped some knowledge on salt and health, but their related behaviors should be improved to reduce the salt intake.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.02.008DOI Listing
March 2020

[Usage status of salt control spoon and influencing factors among students' caregivers of four cities in China in 2014].

Wei Sheng Yan Jiu 2020 Jan;49(1):44-50

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To analysis the usage status of salt control spoon and explore its influencing factors among student caregivers of four cities.

Methods: The data of 2014 salt knowledge, attitude, practice(KAP) survey among urban residents in China was used, which a total of 12 848 student caregivers(male 4862, female 7986) were recruited from Guangzhou, Wuhan, Chengdu and Shenyang by stratified random cluster sampling method. A self-filling questionnaire survey was used to collect information about knowledge of salt and usage of salt control spoon. Logistic regression model was used to explore influencing factors of salt control spoon usage effected by gender, education level, family income, salt related knowledge, etc. .

Results: The proportion of student caregivers who didn't know salt control spoon was 33. 3%, and 41. 9% of them knew salt control spoon but hadn't it. Only 24. 8% of them have heard of salt control spoon and had it at home. The proportion of having not heard salt control spoon among Wuhan, male, junior high school and below, other occupation and low family income group was higher than other groups. The proportion of having salt control spoon of groups in Guangzhou, female, high school and above, professional technical and clerical personnel, high family income was higher than other groups, and the difference was statistically significant(P<0. 01). Among the student caregivers having salt control spoon, the proportion of did not used salt control spoon, used it occasionally or used it without controlling the quantity and used it often and controlled the quantity was 10. 0%, 60. 6% and 29. 5%, respectively. The student caregivers of Shenyang, male, high family income groups used salt control spoon and controlled the quantity more often than the other groups, and the difference was statistically significant(P<0. 05). As shown in multivariate logistic regression analysis, the OR(95%CI) of high school and above, high income level, knowing the recommended intake of salt, knowing that high salt diet can cause high blood pressure on the influence of having salt control spoon was 1. 328(1. 221-1. 445), 1. 268(1. 029-1. 563), 1. 243(1. 145-1. 348)and 0. 838(0. 763-0. 921), respectively. Among the student caregivers who having salt control spoon, the OR(95%CI) of knowing the recommended intake of salt, knowing that high salt diet can cause high blood pressure, and source of salt control spoon(community/unit, friends/relatives) on the influence of using salt control spoon was 1. 473(1. 259-1. 722), 1. 249(1. 040-1. 501)and 0. 505(0. 415-0. 615)/0. 603(0. 441-0. 823), respectively.

Conclusion: There still existed lower proportion of awareness and usage of salt control spoon.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.01.008DOI Listing
January 2020