Publications by authors named "Xiaomin Li"

447 Publications

Effect of Acupuncture on Simple Obesity and Serum Levels of Prostaglandin E and Leptin in Sprague-Dawley Rats.

Comput Math Methods Med 2021 4;2021:6730274. Epub 2021 Oct 4.

Department of Traditional Chinese Medicine II Ward, Huaibei People's Hospital, Huaibei, 235000 Anhui Province, China.

Aim: The study is aimed at investigating the curative effect of acupuncture on simple obesity and its influence on serum levels of prostaglandin E and leptin in Sprague-Dawley (SD) rats.

Methods: In the study, there are 50 male SD rats. We took 10 as healthy controls and fed 40 with a diet of high fat for 8 weeks. After the 40 rat model was established successfully, we fed 10 rats in the model group with a normal diet and treated 10 rats in the acupuncture group by acupuncture. During the experiment, the body fat and body length of rats were measured weekly, and Lee's index was calculated. After the treatment, the levels of leptin, prostaglandin E, C-reactive protein (CRP), triacylglycerol (TG), cholesterol (CHO), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were detected, and the liver fat morphology was observed by electron microscope.

Results: Acupuncture significantly downregulated the serum levels of CRP, TG, CHO, LDL, leptin, and prostaglandin E and upregulated the serum levels of HDL in rats with simple obesity.

Conclusion: On basis of these results, it was found that acupuncture could boost fat metabolism and weight loss by inhibiting the production of leptin and prostaglandin E.
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http://dx.doi.org/10.1155/2021/6730274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8505091PMC
October 2021

Caenorhabditis elegans as a Model System for Discovering Bioactive Compounds against Polyglutamine-mediated Neurotoxicity.

J Vis Exp 2021 Sep 21(175). Epub 2021 Sep 21.

Institute for Food Nutrition and Human Health, School of Food Science and Engineering, South China University of Technology;

Age-related misfolding and aggregation of pathogenic proteins are responsible for several neurodegenerative diseases. For example, Huntington's disease (HD) is principally driven by a CAG nucleotide repeat that encodes an expanded glutamine tract in huntingtin protein. Thus, the inhibition of polyglutamine (polyQ) aggregation and, in particular, aggregation-associated neurotoxicity is a useful strategy for the prevention of HD and other polyQ-associated conditions. This paper introduces generalized experimental protocols to assess the neuroprotective capacity of test compounds against HD using established polyQ transgenic Caenorhabditis elegans models. The AM141 strain is chosen for the polyQ aggregation assay as an age-associated phenotype of discrete fluorescent aggregates can be easily observed in its body wall at the adult stage due to muscle-specific expression of polyQ::YFP fusion proteins. In contrast, the HA759 model with strong expression of polyQ-expanded tracts in ASH neurons is used to examine neuronal death and chemoavoidance behavior. To comprehensively evaluate the neuroprotective capacity of target compounds, the above test results are ultimately presented as a radar chart with profiling of multiple phenotypes in a manner of direct comparison and direct viewing.
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http://dx.doi.org/10.3791/63081DOI Listing
September 2021

Donor-π-Acceptor Heterosystem-Functionalized Porous Hollow Carbon Microsphere for High-Performance Li-S Cathode Materials with S up to 93 wt.

ACS Appl Mater Interfaces 2021 Oct 10. Epub 2021 Oct 10.

Shanghai Electrochemical Energy Devices Research Center, School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Lithium-sulfur (Li-S) batteries, as a prospective energy storage system, are still plagued by many problems that prevent them from their application, especially the low content of sulfur in the cathode. Herein, a cathode material with S up to 93 wt % is designed via a hollow donor-π-acceptor heterosystem, which combines catalytic sites, adsorption sites, and good conductivity together. Following this guidance, a hollow porous carbon sphere is prepared with CoO particles and single V atoms decorated on it (Co/V-HPCS), providing ultrahigh volumetric space for sulfur. Even the electrode made of sulfur-loaded Co/V-HPCS (Co/[email protected]) has a high content of 90 wt % (sulfur content in the electrode is ∼83.5 wt %), and the cathode exhibits an excellent discharge capacity of 575.2 mAh g under 0.2C after 100 cycles. With careful analysis by means of a high-resolution transmission electron microscope (HRTEM), the catalytic amounts of CoO particles and single V atoms loaded on the carbon shell are confirmed, which endows the material with outstanding catalytic ability to transfer sulfur and excellent adsorption of polysulfides. This concept of the cathode material increases the possibility of advanced long-life Li-S batteries with high tap density and high energy density.
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http://dx.doi.org/10.1021/acsami.1c15133DOI Listing
October 2021

Highly Efficient Uptake of TcO by Imidazolium-Functionalized Wood Sawdust.

ACS Omega 2021 Oct 23;6(39):25672-25679. Epub 2021 Sep 23.

School of Nuclear Science and Technology, Lanzhou University, Lanzhou 730000, China.

Tc is a radioactive fission product, mainly in the form of TcO , with good solubility and mobility in the environment. The development of effective and inexpensive materials to remove TcO from nuclear industry wastewater or contaminated water is significant. Wood sawdust is a byproduct of the wood processing industry and is an abundant, low-cost, and sustainable material. The mesostructure of wood consists of numerous hollow cells that are joined endwise to form an interconnected channel matrix capable of rapid transfer of ions. Imidazolium-functionalized wood sawdust (IM-WS) was synthesized using natural wood sawdust by a two-step reaction. It has excellent properties of TcO /ReO adsorption including rapid adsorption dynamics (30 s to equilibrium), good adsorption stability (pH 3-9), high selectivity (adsorption of 45.4 Re % in 1000 times excess of NO ions, 76.6 Re % in 6000 times excess of SO ions, and 92.2 Tc % in a simulated mixed solution; after adsorption, the concentration of TcO decreased to 0.056 ppb from the initial concentration of 12.09 ppb in 1000 times excess of SO ), and in particular low production costs. These characteristics give it great prospects for low-level radioactive wastewater treatment and environmental remediation.
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http://dx.doi.org/10.1021/acsomega.1c03784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495850PMC
October 2021

Direct oral anticoagulants versus vitamin K antagonists for patients with left ventricular thrombus.

Ann Palliat Med 2021 Sep;10(9):9427-9434

Department of Cardiovascular Medicine, Shanxi Bethune Hospital, Third Clinical Medical College of Shanxi Medical University, Taiyuan, China.

Background: A retrospective cohort study was conducted to compare the efficacy and safety of direct oral anticoagulants (DOACs) and vitamin K antagonists (VKAs) in the treatment of patients with left ventricular thrombus (LVT).

Methods: Consecutive patients admitted to our institution with LVT between February 2009 and December 2020 and treated with either DOACs or VKAs were considered for inclusion in this study. The outcomes included stroke or systemic embolism (SSE), thrombus resolution, and bleeding events.

Results: Eighty-seven patients with LVT were identified. Of these, 25 patients were treated with DOACs and 62 patients were treated with VKA. The average follow-up period was 2.37±2.1 years. DOACs were associated with similar incidences of stroke (4.0% vs. 4.8%; P=0.158), systemic embolism (0% vs. 1.6%; P=0.906), SSE (4.0% vs. 6.5%; P=0.657), thrombus resolution (76.0% vs. 74.2%; P=0.057), and blooding events (4.0% vs. 3.2%; P=0.858) as compared to VKAs. In the univariate logistic regression analysis, there was a significant difference between the DOAC and VKA groups in the incidence of SSE when antiplatelets were controlled [odds ratio (OR) =0.34, 95% confidence interval (CI): 0.21, 0.98; P=0.027]. However, in the multivariate analysis, antiplatelets had no significant effect on the outcome (OR =0.41, 95% CI: 0.36, 1.54; P=0.366).

Conclusions: DOACs had similar efficacy and safety to VKAs in the treatment of patients with LVT. Randomized controlled trials should be conducted to verify our findings.
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http://dx.doi.org/10.21037/apm-21-1683DOI Listing
September 2021

NMN recruits GSH to enhance GPX4-mediated ferroptosis defense in UV irradiation induced skin injury.

Biochim Biophys Acta Mol Basis Dis 2021 Oct 6:166287. Epub 2021 Oct 6.

National Translational, Science Center for Molecular Medicine, Fourth Military Medical University, Xi'an 710032, China. Electronic address:

Oxidative stress and lipid peroxidation are major causes of skin injury induced by ultraviolet (UV) irradiation. Ferroptosis is a form of regulated necrosis driven by iron-dependent peroxidation of phospholipids and contributes to kinds of tissue injuries. However, it remains unclear whether the accumulation of lipid peroxides in UV irradiation-induced skin injury could lead to ferroptosis. We generated UV irradiation-induced skin injury mice model to examine the accumulation of the lipid peroxides and iron. Lipid peroxides 4-HNE, the oxidative enzyme COX2, the oxidative DNA damage biomarker 8-OHdG, and the iron level were increased in UV irradiation-induced skin. The accumulation of iron and lipid peroxidation was also observed in UVB-irradiated epidermal keratinocytes without actual ongoing ferroptotic cell death. Ferroptosis was triggered in UV-irradiated keratinocytes stimulated with ferric ammonium citrate (FAC) to mimic the iron overload. Although GPX4 protected UVB-injured keratinocytes against ferroptotic cell death resulted from dysregulation of iron metabolism and the subsequent increase of lipid ROS, keratinocytes enduring constant UVB treatment were markedly sensitized to ferroptosis. Nicotinamide mononucleotide (NMN) which is a direct and potent NAD precursor supplement, rescued the imbalanced NAD/NADH ratio, recruited the production of GSH and promoted resistance to lipid peroxidation in a GPX4-dependent manner. Taken together, our data suggest that NMN recruits GSH to enhance GPX4-mediated ferroptosis defense in UV irradiation-induced skin injury and inhibits oxidative skin damage. NMN or ferroptosis inhibitor might become promising therapeutic approaches for treating oxidative stress-induced skin diseases or disorders.
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http://dx.doi.org/10.1016/j.bbadis.2021.166287DOI Listing
October 2021

Improving the functionality of chitosan-based packaging films by crosslinking with nanoencapsulated clove essential oil.

Int J Biol Macromol 2021 Oct 6. Epub 2021 Oct 6.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230601, China. Electronic address:

The study aimed to obtain chitosan composite films with gratifying physical and functional properties. First, we developed a Pickering emulsion containing clove essential oil (CEO)-loaded nanoparticles with 1:2 (w/w) zein and sodium caseinate (NaCas). We found that in this ratio, the CEO-loaded zein-NaCas (C/ZN) nanoparticles had smaller particle size, proper polydispersity index (PDI) and zeta potential as well as higher encapsulation efficiency. Then, the acquired C/ZN nanoparticles were incorporated into chitosan film at three levels (0.2%, 0.4% and 0.6%), reducing the water vapor permeability to 4.62 × 10 g·s·m·Pa. Also, the tensile strength and break elongation of chitosan films were increased, reaching 38.67 MPa and 1.56%, respectively. The infrared spectroscopy verified that the intermolecular hydrogen bonds exist between chitosan and C/ZN nanoparticles. The chitosan composite films showed a controlled-release property of CEO in 96 h. Finally, the chitosan composite films showed the improved antibacterial property by creating larger inhibition zones against Escherichia coli (3.29 mm) and Staphylococcus aureus (6.15 mm). In general, we improved the water resistance, light blocking, mechanical strength, controlled-release and antibacterial properties of chitosan film with C/ZN nanoparticles. The current edible antibacterial films have great potential on applications for food preservation and food delivery system.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.197DOI Listing
October 2021

Occurrence, profiles, and ecotoxicity of poly- and perfluoroalkyl substances and their alternatives in global apex predators: A critical review.

J Environ Sci (China) 2021 Nov 4;109:219-236. Epub 2021 May 4.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; School of Environment, Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences, Hangzhou 310000, China; Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Institute of Environment and Health, Jianghan University, Wuhan 430010, China. Electronic address:

Certain poly- and perfluoroalkyl substances (PFASs) exhibit significant bioaccumulation/biomagnification behaviors in ecosystems. PFASs, such as perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorohexanesulfonic acid (PFHxS) and related precursors, have elicited attention from both public and national regulatory agencies, which has resulted in worldwide restrictions on their production and use. Apex predators occupy the top trophic positions in ecosystems and are most affected by the biomagnification behavior of PFASs. Meanwhile, the long lifespans of apex predators also lead to the high body burden of PFASs. The high body burden of PFASs might be linked to adverse health effects and even pose a potential threat to their reproduction. As seen in previous reviews of PFASs, knowledge is lacking between the current stage of the PFAS body burden and related effects in apex predators. This review summarized PFAS occurrence in global apex predators, including information on the geographic distribution, levels, profiles, and tissue distribution, and discussed the trophic transfer and ecotoxicity of PFASs. In the case where legacy PFASs were restricted under international convention, the occurrence of novel PFASs, such as 6:2 chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) and perfluoroethylcyclohexane sulfonate (PFECHS), in apex predators arose as an emerging issue. Future studies should develop an effective analytical method and focus on the toxicity and trophic transfer behavior of novel PFASs.
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http://dx.doi.org/10.1016/j.jes.2021.03.036DOI Listing
November 2021

Activation of TLR2 heterodimers-mediated NF-κB, MAPK, AKT signaling pathways is responsible for Vibrio alginolyticus triggered inflammatory response in vitro.

Microb Pathog 2021 Sep 30:105219. Epub 2021 Sep 30.

Jiangsu Key Laboratory of Marine Biological Resources and Environment, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China; Key Laboratory of Zoonosis Research, College of Veterinary Medicine, Jilin University, Changchun, 130062, China. Electronic address:

Vibrio alginolyticus is an important zoonotic marine pathogenic bacterium. Previous studies on the mechanism of innate immune against V. alginolyticus infection have been limited to aquatic animals, however, how V. alginolyticus activates mammalian immune cells has not been fully clarified. Here, ELISA combined RT-qPCR assays were used to detect the secretion and transcription level of pro-inflammatory cytokines and TLRs during V. alginolyticus infection of mice peritoneal macrophages (PMϕs). Western blotting was used to explore the phosphorylation levels of p38, JNK, ERK, AKT and NF-κB protein. Immunofluorescence assay was used to determine the location of NF-κB protein. Inhibition assay was used to study the role of up-regulated TLR in activated signaling pathways and the role of these pathways in the release of pro-inflammatory cytokines. Our data showed that V. alginolyticus can up-regulate the expression levels of IL-1β, IL-6, IL-12 and TNF-α in PMϕs. In addition, V. alginolyticus stimulation activated the phosphorylation of p38, JNK and ERK were TLR2 heterodimers-dependent, whereas inhibitors of SB203580 (p38), SCH772984 (ERK) and SP600125 (JNK) significantly reduced IL-1β, IL-6, IL-12 and TNF-α production. We further revealed that V. alginolyticus activated the signaling pathways of AKT via TLR2 heterodimers. The inhibitor of MK-2206 2HCl (AKT) negatively regulated the IL-1β, IL-6 and TNF-α release levels. Moreover, V. alginolyticus infection of PMϕs resulted in TLR2 heterodimers-mediated activation of NF-κB and induced translocation of phosphorylated NF-κB protein from the cytoplasm into the nucleus via IκBα degradation. V. alginolyticus induced IL-1β, IL-6, IL-12 and TNF-α release were blocked by the specific NF-κB inhibitor, BAY 11-7082. Taken together, our results suggested that activation of the TLR2 heterodimers-mediated downstream signaling pathways NF-κB, MAPK and AKT is responsible for inflammatory response during Vibrio alginolyticus infection in vitro.
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http://dx.doi.org/10.1016/j.micpath.2021.105219DOI Listing
September 2021

Effects of Oral Vitamin C Supplementation on Liver Health and Associated Parameters in Patients With Non-Alcoholic Fatty Liver Disease: A Randomized Clinical Trial.

Front Nutr 2021 14;8:745609. Epub 2021 Sep 14.

Department of Nutrition and Food Safety of School of Public Health, Xi'an Jiaotong University, Xi'an, China.

Non-alcoholic fatty liver disease (NAFLD) is now recognized as the most prevalent hepatic disorder worldwide, and an unhealthy lifestyle is the leading risk factor for its occurrence. Vitamin C (VC) has been suggested to protect NAFLD, whereas evidence from randomized controlled trials (RCTs) is sparse. In this study, we aimed to investigate the potential benefits of VC supplementation daily on liver health and associated parameters in patients with NAFLD. In this double-blind, RCT, 84 patients with NAFLD, aged 18-60 years old, were assigned to 12 weeks of oral treatment with either low (250 mg/day, = 26), medium (1,000 mg/day, = 30), or high (2,000 mg/day, = 28) doses of VC supplements. After the intervention, the Medium group had a more significant decrease in aspartate aminotransferase [Medium, -5.00 (-10.25, -1.75) vs. High, -2.50 (-7.75, 0.00), = 0.02] and alanine aminotransferase [Medium, -8.00 (-18.00, -1.75) vs. High, -3.50 (-13.75, 4.25), = 0.05; Medium vs. Low, -3.00 (-9.00, 5.50), = 0.031]. The levels of other indicators of liver health, such as gamma-glutamyl transferase, alkaline phosphatase, total bilirubin, and direct bilirubin were decreased after the intervention but comparable among the three groups and so did the parameters of glucose metabolism, such as fasting insulin, fasting glucose, and homeostasis model assessment for insulin resistance. The plasma level of VC in patients and total adiponectin and high molecular weight (HMW) adiponectin levels were also elevated but not in a dose-dependent manner. Meanwhile, analysis of fecal microbiota composition showed an increase in the alpha diversity (Abundance-based Coverage Estimator (ACE), Shannon, chao1, and Simpson) both in the Low and the Medium groups. A total of 12 weeks of VC supplementation, especially 1,000 mg/day, improved liver health and glucose metabolism in patients with NAFLD. The elevated plasma levels of VC, total and HMW adiponectin, and the improvement of intestinal microbiota may have made some contributions.
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http://dx.doi.org/10.3389/fnut.2021.745609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8478121PMC
September 2021

Effects of microplastics on soil microbiome: The impacts of polymer type, shape, and concentration.

Sci Total Environ 2021 Sep 27;806(Pt 2):150516. Epub 2021 Sep 27.

Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation, College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

Increasing research has recognized that the ubiquitous presence of microplastics in terrestrial environments is undeniable, which potentially alters the soil ecosystem properties and processes. The fact that microplastics with diverse characteristics enter into the soil may induce distinct effects on soil ecosystems. Our knowledge of the impacts of microplastics with different polymers, shapes, and concentrations on soil bacterial communities is still limited. To address this, we examined the effects of spherical microplastics (150 μm) with different polymers (i.e., polyethylene (PE), polystyrene (PS), and polypropylene (PP)) and four shapes of PP microplastics (i.e., fiber, film, foam, and particle) at a constant concentration (1%, w/w) on the soil bacterial community in an agricultural soil over 60 days. Treatments with different concentrations (0.01-20%, w/w) of PP microplastic particles (150 μm) were also included. The bacterial communities in PE and PP treatments showed a similar pattern but separated from those in PS-treated soils, indicating the polymer backbone structure is an important factor modulating the soil bacterial responses. Fiber, foam, and film microplastics significantly affected the soil bacterial composition as compared to the particle. The community dissimilarity of soil bacteria was significantly (R = 0.592, p < 0.001) correlated with the changes of microplastic concentration. The random forest model identified that certain bacteria belonging to Patescibacteria were closely linked to microplastic contamination. Additionally, analysis of the predicted function further showed that microplastics with different characteristics caused distinct effects on microbial community function. Our findings suggested that the idiosyncrasies of microplastics should not be neglected when studying their effects on terrestrial ecosystems.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150516DOI Listing
September 2021

Cadmium uptake and transport processes in rice revealed by stable isotope fractionation and Cd-related gene expression.

Sci Total Environ 2021 Sep 28;806(Pt 2):150633. Epub 2021 Sep 28.

CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China.

Multiple processes are involved in Cd transfer in rice plants, including root uptake, xylem loading, and immobilization. These processes can be mediated by membrane transporters and can alter Cd speciation by binding Cd to different organic ligands. However, it remains unclear which processes control Cd transport in rice in response to different watering conditions in soil. Herein, Cd isotope fractionation and Cd-related gene expression were employed to investigate the key regulatory mechanisms during uptake, root-to-shoot, and stem-to-leaf transport of Cd in rice grown in pot experiments with Cd-contaminated soil under flooded and non-flooded conditions, respectively. The results showed that soil flooding decreased the Cd concentration in soil porewater and, thereby, Cd uptake and transport in rice. Cd isotopes fractionated negatively from soil porewater to the whole rice (flooded: ∆Cd = -0.15‰, non-flooded: ∆Cd = -0.39‰), suggesting that Cd transporters preferentially absorbed light Cd isotopes. The non-flooded treatment revealed an upregulated expression of OsNRAMP1 and OsNRAMP5 genes compared to the flooded treatment, which may partially contribute to its more pronounced porewater-to-rice fractionation. Cd isotopes fractionated positively from roots to shoots under flooded conditions (∆Cd = 0.19‰). However, a reverse direction of fractionation was observed under non-flooded conditions (∆Cd = -0.67‰), which was associated with the substantial upregulation of CAL1 in roots, facilitating xylem loading of Cd-CAL1 complexes with lighter isotopes. After being transported to the shoots, the majority of Cd were detained in stems (44%-55%), which were strongly enriched in lighter isotopes than in the leaves (∆Cd = 0.77 to 1.01‰). Besides the Cd-CAL1 transported from the roots, the expression of OsPCS1 and OsHMA3 in the stems could also favor the enrichment of Cd-PCs with lighter isotopes, leaving heavier isotopes to be transported to the leaves. The higher expression levels of OsMT1e in older leaves than in younger leaves implied that Cd immobilization via binding to metallothioneins like OsMT1e may favor the enrichment of lighter isotopes in older leaves. The non-flooded treatment showed lighter Cd isotopes in younger leaves than the flooded treatment, suggesting that more Cd-CAL1 in the stems and Cd-PCs in the older leaves might be transported to the younger leaves under non-flooded conditions. Our results demonstrate that isotopically light Cd can be preferentially transported from roots to shoots when more Cd is absorbed by rice under non-flooded conditions, and isotope fractionation signature together with gene expression quantification has the potential to provide a better understanding of the key processes regulating Cd transfer in rice.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150633DOI Listing
September 2021

Near-infrared manipulation of multiple neuronal populations via trichromatic upconversion.

Nat Commun 2021 09 27;12(1):5662. Epub 2021 Sep 27.

Department of Chemistry, Institutes of Brain Science, State Key Laboratory of Molecular Engineering of Polymers, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai, 200433, P. R. China.

Using multi-color visible lights for independent optogenetic manipulation of multiple neuronal populations offers the ability for sophisticated brain functions and behavior dissection. To mitigate invasive fiber insertion, infrared light excitable upconversion nanoparticles (UCNPs) with deep tissue penetration have been implemented in optogenetics. However, due to the chromatic crosstalk induced by the multiple emission peaks, conventional UCNPs or their mixture cannot independently activate multiple targeted neuronal populations. Here, we report NIR multi-color optogenetics by the well-designed trichromatic UCNPs with excitation-specific luminescence. The blue, green and red color emissions can be separately tuned by switching excitation wavelength to match respective spectral profiles of optogenetic proteins ChR2, C1V1 and ChrimsonR, which enables selective activation of three distinct neuronal populations. Such stimulation with tunable intensity can not only activate distinct neuronal populations selectively, but also achieve transcranial selective modulation of the motion behavior of awake-mice, which opens up a possibility of multi-color upconversion optogenetics.
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http://dx.doi.org/10.1038/s41467-021-25993-7DOI Listing
September 2021

AhR-mediated CYP1A1 and ROS overexpression are involved in hepatotoxicity of decabromodiphenyl ether (BDE-209).

Toxicol Lett 2021 Sep 24;352:26-33. Epub 2021 Sep 24.

Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.

Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants. They are constantly detected in terrestrial, ocean, and atmospheric systems, and it is of particular concern that these fat-soluble xenobiotics may have a negative impact on human health. This study aimed to evaluate the toxic effect and underlying mechanism of decabromodiphenyl ether (BDE-209) on human liver in a HepG2 cell model. The results showed that BDE-209 significantly induced HepG2 cells apoptosis, increased intracellular reactive oxygen species (ROS), disturbed [Ca ] homeostasis and mitochondrial membrane potential (MMP), and caused nuclear shrinkage and DNA double-strand breaks. BDE-209 also significantly decreased the activities of antioxidant parameters, superoxide dismutase (SOD), total antioxygenic capacity (T-AOC), glutathione (GSH), and total glutathione (T-GSH). The up-regulation of the Aryl hydrocarbon receptor (AhR)/cytochrome P4501A1 (CYP1A1) signaling pathway indicates that after long-term and high-dose exposure, BDE-209 may be a liver carcinogen. Interestingly, HepG2 cells attempt to metabolize BDE-209 through the Nrf2-mediated antioxidant pathway. These findings help elucidate the mechanisms of BDE-209-induced hepatotoxicity in humans.
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http://dx.doi.org/10.1016/j.toxlet.2021.09.008DOI Listing
September 2021

Adalimumab Therapy Restores the Gut Microbiota in Patients With Ankylosing Spondylitis.

Front Immunol 2021 1;12:700570. Epub 2021 Sep 1.

Rheumatology and Immunology Department, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Growing evidence suggests that the gut microbiota is involved in the initiation and progression of ankylosing spondylitis (AS). In this study, we aimed to explore the gut microbiome alterations during adalimumab therapy and verify microbiome biomarkers predicting treatment response. By evaluating the gut microbial features of 30 AS patients before and after adalimumab therapy for 6 months and 24 healthy controls, we confirmed that the microbiome was restored remarkably after 6 months of adalimumab therapy in AS patients. We then compared the baseline gut microbiome of 22 adalimumab responders with 8 non-responders, a higher abundance of was revealed in the latter, although no statistical difference was found after adjusting for the false discovery rate. These results suggested that adalimumab therapy restored the gut microbiome in AS patients and indicated the utility of gut microbiome to be potential biomarkers for therapeutic evaluation. These findings provided an insight into the development of predictive tools and the establishment of precise medical interventions for clinical practice.
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http://dx.doi.org/10.3389/fimmu.2021.700570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441001PMC
September 2021

Water Management Alters Cadmium Isotope Fractionation between Shoots and Nodes/Leaves in a Soil-Rice System.

Environ Sci Technol 2021 10 14;55(19):12902-12913. Epub 2021 Sep 14.

National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.

The drainage of rice soils increases Cd solubility and results in high Cd concentrations in rice grains. However, plant Cd uptake is limited by sorption to iron plaques, and Cd redistribution in the plant is regulated by the nodes. To better understand the interplay of Cd uptake and redistribution in rice under drained and flooded conditions, we determined stable Cd isotope ratios and the expression of genes coding transporters that can transport Cd into the plant cells in a pot experiment. In soil, both water management practices showed similar patterns of isotope variation: the soil solution was enriched in heavy isotopes, and the root Fe plaque was enriched in light isotopes. In rice, the leaves were heavier (ΔCd = 0.17 to 0.96‰) and the nodes were moderately lighter (ΔCd = -0.26 to 0.00‰) relative to the shoots under flooded conditions, indicating preferential retention of light isotopes in nodes and export of heavy isotopes toward leaves. This is generally reversed under drained conditions (ΔCd = -0.25 to -0.04‰, ΔCd = 0.10 to 0.19‰). The drained treatment resulted in significantly higher expression of and (phloem loading) but lower expression of (vacuolar sequestration) in nodes and flag leaves relative to the flooded treatment. It appeared that OsHMA2 and OsLCT1 might preferentially transport isotopically heavier Cd, and the excess Cd was purposefully retranslocated via the phloem under drained conditions when the vacuoles could not retain more Cd. Cd in seeds was isotopically heavier than that in stems under both water management practices, indicating that heavy isotopes were preferentially transferred toward seeds via the phloem, leaving light isotopes retained in stems. These findings demonstrate that the Fe plaque preferentially adsorbs and occludes light Cd isotopes on the root surface, and distinct water management practices alter the gene expression of key transporters in the nodes, which corresponds to a change in isotope fractionation between shoots and nodes/leaves.
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http://dx.doi.org/10.1021/acs.est.0c04713DOI Listing
October 2021

Correlation Analysis between Mechanical Power and Lung Ultrasound Score and Their Evaluation of Severity and Prognosis in ARDS Patients.

Biomed Res Int 2021 1;2021:4156162. Epub 2021 Sep 1.

Department of Emergency Medicine, Lianyungang Clinical College of Nanjing Medical University, the First People's Hospital of Lianyungang, Lianyungang, China.

Methods: A total of 121 patients with moderate to severe ARDS admitted to the intensive care unit (ICU) from June 2017 to April 2020 and treated with invasive mechanical ventilation were sequentially included in this study. Their general information was collected, and MP was recorded at 0 h, 24 h, 48 h, and 72 h after admission to the ICU. Professionally trained researchers performed the LUS assessments. Patients were divided into the death and survival groups according to their 28-day prognosis. The trend of MP and LUS at the four time points was analyzed. A receiver operating characteristic curve (ROC) was used to analyze the predictive value of MP and LUS scores at 0 h and 72 h for the prognosis (28-day mortality rate) of patients with moderate to severe ARDS.

Results: 121 patients were included in the analysis, of which 73 were male and 48 were female. When patients entered the ICU, their oxygenation index (: 30885, < 0.01), APACHE II score (: 2.105, < 0.05), and SOFA score (: 4.134, < 0.001) were higher in the death group than the survival group. The death group had significantly higher MP and LUS at each time point (0 h, 24 h, 48 h, and 72 h) compared to the survival group (all < 0.05). There was a significant upward trend over time in the MP and LUS of the death group, contrasting to a significant downward trend in the survival group (all < 0.05). The Pearson correlation analysis showed that MP and LUS were significantly positively correlated at each time point ( values: 0 h: 0.3027; 24 h: 0.3705; 48 h: 0.3902; 72 h: 0.5916; all < 0.01). The ROC curves showed that MP and LUS at 72 h were of significant value in predicting the prognosis of ARDS patients, with areas under the curve of 0.866 ± 0.032 and 0.839 ± 0.037, respectively.

Conclusion: There was a significant correlation between the MP and LUS of ARDS patients at four time points from 0 to 72 h, which has a clinical value in evaluating severity and prognosis.
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http://dx.doi.org/10.1155/2021/4156162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429004PMC
September 2021

Denitrification performance and microbial community of bioreactor packed with PHBV/PLA/rice hulls composite.

Sci Total Environ 2021 Sep 1;803:150033. Epub 2021 Sep 1.

Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing 100084, PR China; Beijing Key Laboratory of Radioactive Wastes Treatment, Tsinghua University, Beijing 100084, PR China. Electronic address:

In this study, a novel biodegradable PHBV/PLA/rice hulls (PPRH) composite was applied and tested as biofilm attachment carrier and carbon source in two bioreactors for biological denitrification process. The denitrification performance, effect of operational conditions and microbial community structure of PPRH biofilm were evaluated. The batch experiment results showed that PPRH-packed bioreactor could completely remove 50 mg L of NO-N at natural pH (ca. 7.5) and room temperature. The continuous flow experiments indicated that high NO-N removal efficiency (77%-99%) was achieved with low nitrite (<0.48 mg L) and ammonia (<0.81 mg L) accumulation, when influent NO-N concentration was 30 mg L and hydraulic retention time was 2-6 h. Furthermore, the microbial community analysis indicated that bacteria belonging to genus Diaphorobacter in phylum Proteobacteria were the most dominant and major denitrifiers in denitrification. In summary, PPRH composite was a promising carbon source for biological nitrate removal from water and wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150033DOI Listing
September 2021

Dioscin attenuates lipopolysaccharide-induced inflammatory myocardial injury through oxidative stress-related pathway.

Ann Palliat Med 2021 Aug;10(8):8827-8836

Department of Cardiovascular Medicine, Shanxi Bethune Hospital, Third Clinical Medical College of Shanxi Medical University, Taiyuan, China.

Background: Lipopolysaccharide (LPS) is one of the main causes of myocardial injury. Dioscin has a protective effect on myocardial injury induced by LPS; however, the biological function and mechanism remain unclear. The purpose of this study was to investigate the effect of dioscin on myocardial injury induced by LPS.

Methods: The myocardial injury model was constructed through LPS treatment of primary rat cardiomyocytes. Cardiomyocytes were treated with different concentrations of dioscin (50, 100, and 200 ng/mL). MTT was used to detect the activity of cardiomyocytes; flow cytometry and TUNEL assay were used to detect apoptosis; and enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). The release of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) was detected according to the kit instructions. The levels of apoptosis-related proteins (Bax, caspase-3, and Bcl 2) and the Nrf2-Keap1 pathway proteins were detected by western blot.

Results: Dioscin significantly reduced LPS-induced cardiomyocyte injury in neonatal rats in a concentration- and time-dependent manner. Dioscin also significantly inhibited cardiomyocyte inflammation and apoptosis induced by LPS. With the increase of dioscin concentration, reactive oxygen species (ROS) and MDA were downregulated, and SOD and GSH were upregulated. Moreover, dioscin inhibited LPS-induced myocardial injury by inhibiting the Nrf2-Keap1 pathway.

Conclusions: Our study suggests that dioscin attenuates LPS-induced myocardial injury through oxidative stress-related pathways.
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http://dx.doi.org/10.21037/apm-21-1613DOI Listing
August 2021

circCDYL2, Overexpressed in Highly Migratory Colorectal Cancer Cells, Promotes Migration by Binding to Ezrin.

Front Oncol 2021 17;11:716073. Epub 2021 Aug 17.

Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Background: Colorectal cancer (CRC) is one of the most common malignancies with high mortality worldwide, particularly due to metastasis. However, there are no clinically available strategies for treating CRC metastasis. Exploring the mechanisms underlying CRC metastasis is the key to improve the treatment of CRC with metastasis.

Methods: In this study, we generated the highly migratory CRC cell subline H-RKO using a repeated transwell migration assay to identify circRNAs involved in CRC migration by high-throughput RNA sequencing. Upregulated circRNAs were validated by RT-qPCR to identify the most elevated circRNA. The expression of this circRNA (circCDYL2) was evaluated in 40 pairs of CRC tissues and four CRC cell lines by RT-qPCR. Transwell migration and wound healing assays were performed to verify the function of circCDYL2 in cell migration. The cellular distribution of circCDYL2 was confirmed using PCR. RNA pulldown and RNA immunoprecipitation were used to confirm the interaction between circCDYL2 and Ezrin. Western blotting, immunohistochemistry, and rescue experiments were used to determine the role of circCDYL2 in regulating Ezrin protein expression and AKT phosphorylation.

Results: Among the candidate circRNAs, circCDYL2 was the highest overexpressed circRNA in H-RKO compared to parental N-RKO cells. Furthermore, circCDYL2 expression was elevated in CRC tissues and cell lines. Gain- and loss-of-function assays indicated that circCDYL2 enhanced the migration of CRC cells. circCDYL2 was located in the cytoplasm of CRC cells and interacted with Ezrin to upregulate its protein levels, resulting in AKT phosphorylation. Ezrin knockdown abrogated the CRC cell migration induced by circCDYL2 overexpression.

Conclusions: Our study demonstrated for the first time that circCDYL2 promotes CRC migration by binding Ezrin and activating the AKT pathway. CircCDYL2 represents a potential therapeutic target for preventing CRC metastasis.
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http://dx.doi.org/10.3389/fonc.2021.716073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416158PMC
August 2021

Chronic Stress Activates PlexinA1/VEGFR2-JAK2-STAT3 in Vascular Endothelial Cells to Promote Angiogenesis.

Front Oncol 2021 16;11:709057. Epub 2021 Aug 16.

Department of Pathology, Chengde Medical College, Chengde, China.

It is known that chronic stress modulates multiple processes in a complex microenvironment, such as angiogenesis and immune function. However, the role of chronic stress inducing tumor angiogenesis and how it contributes to tumor progression are not quite clear. The following study assess psychological state from numerous ambulatory cancer cases (n=332), and chronic stress-related hormone levels were further measured. Here, we show that chronic stress not only causes behavioral changes in human, most importantly attributed to an elevated level of stress-related hormones. To address this, isoprenaline, the agonist of β2-adrenergic receptor (β2-AR), was utilized for simulating chronic stress and demonstrating the mechanism of stress in tumor angiogenesis at molecular level both and . As suggested by this study, isoprenaline promote VEGF autocrine of HUVECs, which can induce plexinA1 and VEGFR2 expression. Moreover, we show that isoprenaline promoted the expression of p-JAK2 and p-STAT3 . The results reveal that, isoprenaline enhances the autocrine of VEGF in HUVECs and up-regulating plexinA1 and VEGFR2 levels, thus activating the phosphorylation of JAK2-STAT3 pathway, the two essential parts during angiogenesis. The present work indicates that, the mechanism of chronic stress in enhancing angiogenesis is probably achieved through activating the plexinA1/VEGFR2-JAK2-STAT3 signal transduction pathway within HUVECs, and this is probably a candidate target for developing a strategy against angiogenesis in cancer.
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http://dx.doi.org/10.3389/fonc.2021.709057DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8415364PMC
August 2021

A pH-responsive Pt-based nanoradiosensitizer for enhanced radiotherapy via oxidative stress amplification.

Nanoscale 2021 Aug 3;13(32):13735-13745. Epub 2021 Aug 3.

Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.

Tumor radioresistance is a major issue in radiotherapy. To address it, a pH-responsive nanoradiosensitizer was synthesized employing a simple method. Initially, chloroplatinic acid was reduced by human serum albumin (HSA) to form HSA-wrapped [email protected] nanoparticles (NPs). Subsequently, cinnamicaldehyde (CA) was grafted on [email protected] via aldimine condensation to obtain nanoradiosensitizer [email protected]/CA NPs. CA would be released in tumor cells (pH = 5.5) to induce the production of reactive oxygen species, including HO, ˙OH, etc. The increased decomposition of HO catalyzed by the NPs resulted in enhanced production of oxygen, leading to hypoxia relief of the tumor cells, which is beneficial for radiotherapy. Due to the high X-ray attenuation coefficient of Pt, [email protected]/CA NPs enhance the energy deposition of radiation. Cytotoxicity assay revealed that [email protected]/CA NPs resulted in a cell death rate of 77%, which was 24.4% higher than that of [email protected] NPs even under low-dose X-ray irradiation of 4 Gy. Colony formation assay demonstrated that the sensitization enhancement ratio was 1.37, indicating that [email protected]/CA NPs displayed remarkable radiosensitizing ability. Notably, in vivo results indicated that the NPs could increase the tumor inhibition rate to 91.2% with negligible side effects to normal tissues. These results demonstrate that [email protected]/CA NPs had outstanding tumor curative efficacy and hypotoxicity.
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http://dx.doi.org/10.1039/d1nr02043aDOI Listing
August 2021

Interventional Clinical Trials on Diabetic Peripheral Neuropathy: A Retrospective Analysis.

J Pain Res 2021 26;14:2651-2664. Epub 2021 Aug 26.

Department of Endocrinology, The First Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin, People's Republic of China.

Aims/introduction: Diabetic peripheral neuropathy (DPN) is the most common complication of diabetes. At present, there is no comprehensive summary of the clinical trials related to DPN. In this article, we summarized the basic characteristics of the interventional clinical trials pertaining to DPN to determine the current status of research in this field and the existing issues.

Materials And Methods: We searched the World Health Organization International Clinical Trial Registration Platform (ICTRP), PubMed and Web of Science for clinical trials from 2005 to April 2021 and extracted 149 registered and 459 published clinical trials on DPN. We summarized the characteristics of the clinical trials, including the source registration, recruitment status, stage, age group, allocation method, intervention, end point classification, funding source, and treatment.

Results: After excluding noninterventional and nontreatment trials, 149 registered clinical trials out of 292 records from 12 registration centers and 459 published articles were included in this study. Among the registered trials, 43% had been completed, and 34.4% had been published in peer-reviewed journals. Among these trials, more than half used random allocation and blinded placebo-controlled methodologies. A total of 40.3% of the trials were multicenter studies, 63.8% of the treatments were drug therapies, and the endpoint classifications of 49% were efficacy and safety. Of the 459 published interventional clinical trials on DPN, 69.7% of the trials used drug treatments; more than half were randomized, double-blind, placebo-controlled clinical trials; 94.1% had positive outcomes; 46.4% had a target size of 50; and 22.9% were multicenter.

Conclusion: This paper systematically summarizes the current status of interventional trials on DPN registered in the ICTRP and published clinical trials and provides a reference for the development of high-quality intervention strategies for DPN in the future.
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http://dx.doi.org/10.2147/JPR.S320364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8405224PMC
August 2021

Tumor acid microenvironment-activated self-targeting & splitting gold nanoassembly for tumor chemo-radiotherapy.

Bioact Mater 2022 Jan 10;7:377-388. Epub 2021 Jun 10.

Key Laboratory of Functional Polymer Materials of Ministry of Education, College of Chemistry, Nankai University, Tianjin, 300071, China.

Low accumulation and penetration of nanomedicines in tumor severely reduce therapeutic efficacy. Herein, a pH-responsive gold nanoassembly is designed to overcome these problems. Polyethylene glycol linked raltitrexed (RTX, target ligand and chemotherapy drug) and two tertiary amine molecules (1-(2-aminoethyl) pyrrolidine and -dibutylethylenediamine) are modified on the surface of the 6-nm gold nanoparticles by lipoic acid to form gold nanoassembly defined as Au-NNP(RTX). The Au-NNP (RTX) nanoassembly could remain at about 160 nm at the blood circulation (pH 7.4), while split into 6-nm gold nanoparticles due to tertiary amine protonation at tumor extracellular pH (pH 6.8). This pH-responsive disassembly behavior endows Au-NNP(RTX) better tumor tissue permeability through the better diffusion brought by the size reduction. Meanwhile, after disassembly, more RTXs on the surface of gold nanoparticles are exposed from the shielded state of assembly along with 2.25-fold augment of cellular uptake capability. Most importantly, the results show that Au-NNP(RTX) possesses of high tumor accumulation and effective tumor penetration, thereby enhancing the tumor chemo-radiotherapy efficiency.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379383PMC
January 2022

Key Compounds and Metabolic Pathway Responsible for the Browning in Dangshan Pear ( spp.) Wine.

J Agric Food Chem 2021 Sep 25;69(35):10311-10320. Epub 2021 Aug 25.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, P. R. China.

Based on hydrophilic interaction liquid chromatography (HILIC) liquid chromatography-mass spectrometry (LC-MS), untargeted differential metabolomics analysis was performed on the pear wine samples before and after browning to determine the key compounds that affect the browning of Dangshan pear wine. A total of 196 significantly differential metabolites were found, 22 of which might be related to the browning of Dangshan pear wine. d-(+)-glucose, l-phenylalanine, l-norleucine, methionine, d-(+)-proline, aloin, and rutin were the key differential metabolites in pear wine before and after browning. The Maillard reaction of d-(+)-glucose, l-norleucine, methionine, and the oxidation of aloin played critical roles in the browning of Dangshan pear wine. The reaction of aloin and glucose to form 5-hydroxyaloin A, 7-hydroxyaloin B, and elgonica-dimer A was one of the important metabolic pathways in which the phenolic compounds formed anthraquinone during the browning process of Dangshan pear wine.
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http://dx.doi.org/10.1021/acs.jafc.1c03966DOI Listing
September 2021

Peptide vaccine-conjugated mesoporous carriers synergize with immunogenic cell death and PD-L1 blockade for amplified immunotherapy of metastatic spinal.

J Nanobiotechnology 2021 Aug 12;19(1):243. Epub 2021 Aug 12.

Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, People's Republic of China.

The clinical treatment of metastatic spinal tumor remains a huge challenge owing to the intrinsic limitations of the existing methods. Programmed cell death protein 1 (PD1)/programmed cell death ligand 1 (PD-L1) pathway blockade has been explored as a promising immunotherapeutic strategy; however, their inhibition has a low response rate, leading to the minimal cytotoxic T cell infiltration. To ameliorate the immunosuppressive microenvironment of intractable tumor and further boost the efficacy of immunotherapy, we report an all-round mesoporous nanocarrier composed of an upconverting nanoparticle core and a large-pore mesoporous silica shell (UCMS) that is simultaneously loaded with photosensitizer molecules, the IDO-derived peptide vaccine AL-9, and PD-L1 inhibitor. The IDO-derived peptide can be recognized by the dendritic cells and presented to CD8 cytotoxic T cells, thereby enhancing the immune response and promoting the killing of the IDO-expressed tumor cells. Meanwhile, the near-infrared (NIR) activated photodynamic therapy (PDT) could induce immunogenic cell death (ICD), which promotes the effector T-cell infiltration. By combining the PDT-elicited ICD, peptide vaccine and immune checkpoint blockade, the designed [email protected] successfully potentiated local and systemic antitumor immunity and reduced the progression of metastatic foci, demonstrating a synergistic strategy for cancer immunotherapy.
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http://dx.doi.org/10.1186/s12951-021-00975-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8362242PMC
August 2021

Predictive Value of Pretreatment Peripheral Neutrophil-to-Lymphocyte Ratio for Response to Neoadjuvant Chemotherapy and Breast Cancer Prognosis.

Cancer Manag Res 2021 28;13:5889-5898. Epub 2021 Jul 28.

Department of Breast Surgery, West China Hospital, Sichuan University, Chengdu, People's Republic of China.

Background: Neutrophil-to-lymphocyte ratio (NLR) is connected with the response to neoadjuvant chemotherapy (NAC) and prognosis. In addition, residual lymph node burden after NAC is likely important for prognosis. However, most studies have focused on the predictive value of NLR for NAC pathological complete response (pCR) rate. The relationship between NLR and post-operative residual lymph node ratio (LNR), and their prognostic values remain to be determined.

Methods: We retrospectively studied 282 patients with breast cancer who underwent curative surgery after NAC from 2008 to 2018. We collected pretreatment NLR in peripheral blood, the response to NAC, and the amount of axillary lymph nodes (positive and total) from patients who received axillary lymph node dissection (ALND). We followed up all patients from 2 to 116 months, with an average of 63 months. We analyzed the predictive value of pretherapeutic NLR in peripheral blood on the response of NAC, including pCR rate and postoperative LNR. The prognostic value of NLR and LNR was also analyzed.

Results: A pCR was achieved in 20 (27.0%) of 74 patients with low NLR, and 34 (16.3%) of 208 with high NLR ( = 0.045). In luminal A and luminal B tumors, patients with high NLR tended to have elevated LNR (LNR>0.5; =0.041). In Kaplan-Meier analysis, overall survival of patients with low NLR (NLR < 1.8; = 0.033) was longer than that of patients with high NLR (NLR ≥ 1.8). Moreover, by multivariable analysis, LNR was negatively correlated with overall survival ( < 0.05) and disease-free survival (DFS) ( < 0.05).

Conclusion: pCR rate, post-operative remaining lymph node involvement and overall survival in all patients who received NAC may be predicted by NLR. Low NLR and LNR may suggest favorable outcomes.
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http://dx.doi.org/10.2147/CMAR.S313123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326279PMC
July 2021

Migratory fishbones in the pharynx: A report of two cases.

Clin Case Rep 2021 Jul 26;9(7):e04548. Epub 2021 Jul 26.

Department of Otolaryngology Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University) Zhuhai China.

The possibility of fishbone migration into the surrounding tissues, especially in cases where it cannot be identified on routine inspection. Early diagnosis of migratory fishbone and therapeutic management are essential for optimal patient survival.
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http://dx.doi.org/10.1002/ccr3.4548DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312238PMC
July 2021

Corrigendum: Exploring Physiological Linkage in Same-Sex Male Couples.

Front Psychol 2021 13;12:720779. Epub 2021 Jul 13.

Department of Family Studies and Human Development, The University of Arizona, Tucson, AZ, United States.

[This corrects the article DOI: 10.3389/fpsyg.2020.619255.].
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http://dx.doi.org/10.3389/fpsyg.2021.720779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8314209PMC
July 2021

BDE-47 induces nephrotoxicity through ROS-dependent pathways of mitochondrial dynamics in PK15 cells.

Ecotoxicol Environ Saf 2021 Oct 26;222:112549. Epub 2021 Jul 26.

Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China. Electronic address:

2,2',4,4'-tetrabromodiphenyl ether (BDE-47)-induced nephrotoxicity is closely associated with oxidative stresses and mitochondrial abnormalities. Mitochondrial fusion and fission dynamics are crucial for maintaining mitochondrial and cellular physiological homeostasis. However, the detailed mechanisms through which BDE-47 disrupts this dynamic and contributes to renal injuries are still not fully understood. The porcine kidney-15 (PK15) cell line, a well-defined in vitro animal renal toxicological model, was exposed to BDE-47 with concentrations of 12.5, 25, 50, and 100 μM, respectively. Cell viability, the levels of reactive oxygen species (ROS) and adenosine triphosphate (ATP), the mitochondrial membrane potential (MMP), and the expression levels of key mitochondrial fusion and fission proteins were assessed. BDE-47 reduced cell viability and disrupted mitochondrial dynamics by inhibiting mitochondrial fusion and fission simultaneously, leading to MMP decreases, ROS overgeneration, ATP depletion, and cellular disintegration in a dose-dependent manner. Additionally, the mitochondrial division inhibitor (Mdivi-1) with the concentration of 20 μM observed to restore the downregulation of mitochondrial fusion and fission proteins, alleviate damages in mitochondrial morphology and functionality, correct ROS overproduction, and enable cell survival. The antioxidant N-acety-L-cysteine (NAC) with the concentration of 1 mM also simultaneously reversed the imbalance of mitochondrial dynamics, decreased ROS production, and restored mitochondrial morphology in PK15 cells exposed to BDE-47. Our data provide new insights indicating that BDE-47 disrupts mitochondrial fusion/fission dynamics to induce mitochondrial abnormalities, triggering oxidative stresses and thus contributing to PK15 cell dysfunction. ROS-dependent pathways in mitochondrial dynamics may provide a new avenue for developing effective strategies to protect cells against BDE-47-induced nephrotoxicity.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112549DOI Listing
October 2021
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