Publications by authors named "Xiaomei Zhang"

520 Publications

The super typhoon Lekima (2019) resulted in massive losses in large seagrass (Zostera japonica) meadows, soil organic carbon and nitrogen pools in the intertidal Yellow River Delta, China.

Sci Total Environ 2021 Jun 10;793:148398. Epub 2021 Jun 10.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China; Shandong Province Key Laboratory of Experimental Marine Biology, Qingdao 266071, China. Electronic address:

Seagrass meadows are key ecosystems, and they are among the most threatened habitats on the planet. Increased numbers of extreme climate events, such as hurricanes and marine heatwaves have caused severe damage to global seagrass meadows. The largest Zostera japonica meadows in China are located in the Yellow River Delta. It had a distribution area of 1031.8 ha prior to August 2019 when the Yellow River Delta was severely impacted by the passage of typhoon Lekima. In this study, we compared field data collected before and after the typhoon to determine its impact on seagrass beds in the Yellow River Delta. The super typhoon caused dramatic changes in Z. japonica in the Yellow River Delta, resulting in a greater than 100-fold decrease in distribution area, a greater than 35% loss of soil organic carbon, and a greater than 65% loss of soil total nitrogen in the top 35 cm sediments. Owing to the lack of seeds and overwintering shoots, as well as the small remaining distribution area, recovery was impossible, even though environmental factors were still suitable for species growth. Thus, restoration efforts are required for seagrass meadow recovery. Additionally, the long-term monitoring of this meadow will provide new information on the ecosystem's status and will be useful for future protection.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148398DOI Listing
June 2021

Association of temperature and relative humidity with the growth rate of the coronavirus disease 2019 epidemic.

Am J Transl Res 2021 15;13(6):5943-5955. Epub 2021 Jun 15.

Graduate Institute of Business Administration, College of Management, Fu Jen Catholic University New Taipei, Taiwan.

The effects of temperature and relative humidity on the growth of coronavirus disease 2019 (COVID-19) remain unclear. Data on the COVID-19 epidemic that were analyzed in this study were obtained from the official websites of the National Health Commission of China and the Health Commissions of 31 provinces in China. From January 26 to February 25, 2020, the cumulative number of confirmed COVID-19 cases in each region was counted daily using data from our database. Curve fitting of daily scatter plots of the relationship between epidemic growth rate (GR) with average temperature (AT) and average relative humidity (ARH) was conducted using the loess method. The heterogeneity across days and provinces was calculated to assess the necessity of using a longitudinal model. Fixed-effect models with polynomial terms were developed to quantify the relationship between variations in the GR and AT or ARH. An increased AT markedly reduced the GR when the AT was lower than -5°C, the GR was moderately reduced when the AT ranged from -5°C to 15°C, and the GR increased when the AT exceeded 15°C. ARH increased the GR when it was less than 72% and reduced the GR when it exceeded 72%. The temperature and relative humidity curves were not linearly associated with the GR of COVID-19. The GR was moderately reduced when the AT ranged from -5°C to 15°C. When the AT was lower or higher than -5°C to 15°C, the GR of COVID-19 increased. An increased ARH increased the GR when the ARH was lower than 72% and reduced the GR when the ARH exceeded 72%.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290681PMC
June 2021

Could clinical nursing procedures lead to tracheal cuff pressure drop? A prospective observational study.

J Clin Nurs 2021 Jul 22. Epub 2021 Jul 22.

Nanfang Hospital, Southern Medical University, Guangzhou City, Guangdong Province, China.

Aim: To evaluate the dynamic changes in tracheal cuff pressure before and after four clinical nursing procedures including sputum suction, oral care, atomisation inhalation, and turning over, and thus provide references for the adjustment time of cuff pressure in clinical practice.

Background: Cuff pressure must be kept within the range of 25-30 cmH O to ensure effective ventilation and prevent aspiration, while maintaining tracheal blood flow perfusion.

Design: A prospective observational study.

Methods: The cuff pressure of 56 intubated patients was adjusted to 28-30 cmH O. A cuff pressure monitor was used to continuously monitor cuff pressure changes before and after four clinical nursing procedures (sputum suction, oral care, atomisation inhalation, and turning over) and the cuff pressures at various time points were compared. The semi-quantitative cough strength score (SCSS) was used to evaluate cough strength during sputum suction and the effect of cough strength on cuff pressure during sputum suction. This study followed the STROBE checklist for cross-sectional studies.

Results: The cuff pressures during the four clinical nursing procedures of sputum suction, atomisation inhalation, turning over, and oral care, all temporarily increased (p < 0.001) and decreased to varying degrees 20 min later (p < 0.001). Among them, the cuff pressure rose the highest under a state of moderate or strong coughing during sputum suction (78.38 ± 12.13 cmH O) and dropped the most at 20 min after the procedure (21.71 ± 4.80 cmH O).

Conclusions: The four clinical nursing procedures of sputum suction, atomisation inhalation, turning over, and oral care can all cause different degrees of cuff pressure drop. The decision on whether the cuff pressure needs to be corrected depends on the specific situation.

Relevance To Clinical Practice: During clinical practice, the cuff pressure can be individually corrected according to different clinical nursing procedures, which can increase the qualified rate of cuff pressure and reduce the workload of nurses.
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http://dx.doi.org/10.1111/jocn.15920DOI Listing
July 2021

Effects of Oral Exposure to Mn-Doped ZnS Quantum Dots on Intestinal Tract and Gut Microbiota in Mice.

Front Physiol 2021 6;12:657266. Epub 2021 Jul 6.

Institute of Molecular Medicine, Henan Provincial Engineering Center for Tumor Molecular Medicine, School of Basic Medical Sciences, Henan University, Kaifeng, China.

Mn-doped ZnS quantum dots (QDs) with excellent optical properties have been explored in a wide range of fields. Their potential adverse effects on biological systems and human health should be evaluated before biological application. In the present study, we investigated the effect of Mn-doped ZnS QDs on the intestinal tract and gut microbiota structures at 2 h and 14 days (d) after 14 d repeated oral exposure in mice. Flame atomic absorption spectrophotometry (FAAS), histopathological examination, and transmission electron microscopy (TEM) were used to assess the absorption and toxicity of Mn-doped ZnS QDs on the intestinal tract. The 16S rRNA gene sequencing was used to evaluate the gut microbial communities. Mn-doped ZnS QDs did not accumulate in the duodenum, jejunum, ileum, or colon. The Zn content of feces was not significantly higher than in the control group. No major histological changes were found in these tissues. The intestinal microvilli remained regular, but swelling of mitochondria and endoplasmic reticulum was detected by TEM at 14 d after the last gavage. A total of 2,712 operational taxonomic units (OTUs) were generated. Mn-doped ZnS QDs treatment did not significantly change the α-diversity of Richness, Chao1, Shannon, and Simpson indexes. According to principal component analysis (PCA), Mn-doped ZnS QDs had no effect on the overall structure of the gut microbiota. No significant change occurred at the phylum level, while three genera were downregulated at 2 h and seven changed at 14 d after the last gavage. Our findings revealed that Mn-doped ZnS QDs had a little stimulation of the intestinal tract and gut microbiota, and oral administration may be a safe route for biological application (such as bioimaging and drug delivery).
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http://dx.doi.org/10.3389/fphys.2021.657266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290145PMC
July 2021

Gait-Based Implicit Authentication Using Edge Computing and Deep Learning for Mobile Devices.

Sensors (Basel) 2021 Jul 5;21(13). Epub 2021 Jul 5.

Commonwealth Scientific and Industrial Research Organization (CSIRO), Sandy Bay 7005, Australia.

Implicit authentication mechanisms are expected to prevent security and privacy threats for mobile devices using behavior modeling. However, recently, researchers have demonstrated that the performance of behavioral biometrics is insufficiently accurate. Furthermore, the unique characteristics of mobile devices, such as limited storage and energy, make it subject to constrained capacity of data collection and processing. In this paper, we propose an implicit authentication architecture based on edge computing, coined Edge computing-based mobile Device Implicit Authentication (EDIA), which exploits edge-based gait biometric identification using a deep learning model to authenticate users. The gait data captured by a device's accelerometer and gyroscope sensors is utilized as the input of our optimized model, which consists of a CNN and a LSTM in tandem. Especially, we deal with extracting the features of gait signal in a two-dimensional domain through converting the original signal into an image, and then input it into our network. In addition, to reduce computation overhead of mobile devices, the model for implicit authentication is generated on the cloud server, and the user authentication process also takes place on the edge devices. We evaluate the performance of EDIA under different scenarios where the results show that i) we achieve a true positive rate of 97.77% and also a 2% false positive rate; and ii) EDIA still reaches high accuracy with limited dataset size.
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http://dx.doi.org/10.3390/s21134592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271781PMC
July 2021

Copper Promotes Tumorigenesis by Activating the PDK1-AKT Oncogenic Pathway in a Copper Transporter 1 Dependent Manner.

Adv Sci (Weinh) 2021 Jul 18:e2004303. Epub 2021 Jul 18.

Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA.

Copper plays pivotal roles in metabolic homoeostasis, but its potential role in human tumorigenesis is not well defined. Here, it is revealed that copper activates the phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB, also termed AKT) oncogenic signaling pathway to facilitate tumorigenesis. Mechanistically, copper binds 3-phosphoinositide dependent protein kinase 1 (PDK1), in turn promotes PDK1 binding and subsequently activates its downstream substrate AKT to facilitate tumorigenesis. Blocking the copper transporter 1 (CTR1)-copper axis by either depleting CTR1 or through the use of copper chelators diminishes the AKT signaling and reduces tumorigenesis. In support of an oncogenic role for CTR1, the authors find that CTR1 is abnormally elevated in breast cancer, and is subjected by NEDD4 like E3 ubiquitin protein ligase (Nedd4l)-mediated negative regulation through ubiquitination and subsequent degradation. Accordingly, Nedd4l displays a tumor suppressive function by suppressing the CTR1-AKT signaling. Thus, the findings identify a novel regulatory crosstalk between the Nedd4l-CTR1-copper axis and the PDK1-AKT oncogenic signaling, and highlight the therapeutic relevance of targeting the CTR1-copper node for the treatment of hyperactive AKT-driven cancers.
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http://dx.doi.org/10.1002/advs.202004303DOI Listing
July 2021

Linc00941 regulates esophageal squamous cell carcinoma via functioning as a competing endogenous RNA for miR-877-3p to modulate PMEPA1 expression.

Aging (Albany NY) 2021 07 13;13(13):17830-17846. Epub 2021 Jul 13.

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and the Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, China.

Esophageal squamous cell carcinoma (ESCC) represents one of the most common malignancies and is the fifth leading cause of cancer-related deaths. Long intergenic non-coding RNAs (lincRNAs) have been suggested to be dysregulated in various types of cancers, and a growing number of lincRNAs have been implicated to be functional in the ESCC progression. In this study, we examined the role of linc00941 in the ESCC progression and explored the underlying molecular mechanisms. The bioinformatics analysis identified the up-regulation of linc00941 in the ESCC tissues. Further studies showed that linc00941 was up-regulated in ESCC cell lines. The loss-of-function studies demonstrated that linc00941 knockdown suppressed ESCC cell proliferation, invasion and migration, and also suppressed the tumor growth. Furthermore, bioinformatics prediction along with luciferase reporter assay and RNA immunoprecipitation assay implied that linc00941 acted as a competing endogenous RNA for miR-877-3p, and linc00941 regulated ESCC cell progression via at least targeting miR-877-3p. Subsequently, miR-877-3p targeted prostate transmembrane protein, androgen induced 1 (PMEPA1) 3' untranslated region and repressed PMEPA1 expression in ESCC cells; overexpression of PMEPA1 attenuated the inhibitory effects of linc00941 knockdown on the ESCC cell progression. Linc00941 knockdown suppressed epithelial-mesenchymal transition (EMT) via targeting miR-877-3p/PMEPA1 axis in ESCC cells. In conclusion, our results indicated the oncogenic role of linc00941 in ESCC, and knockdown of linc00941 suppressed ESCC cell proliferation, invasion, migration and EMT via interacting with miR-877-3p/PMEPA1 axis.
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http://dx.doi.org/10.18632/aging.203286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312468PMC
July 2021

Exploring dynamics and associations of dominant lignocellulose degraders in tomato stalk composting.

J Environ Manage 2021 Sep 30;294:113162. Epub 2021 Jun 30.

College of Resource and Environment, Qingdao Agricultural University, Qingdao, 266109, China. Electronic address:

The plant residues of tomato bring pressures to the environment and composting provides a feasible method to treat such agricultural waste. However, little is known about the succession and associations of the dominant lignocellulose degraders in the compost system. To further accelerate the process by inoculating key functional microorganisms, a compost pile composed of tomato stalk with maize straw addition was constructed, and the whole community structure and functions of the dominant were investigated by applying the integrated mata-omics. Results showed that Actinobacteria, Firmicutes, and Ascomycota dominated and drove the assembly of the co-occurrence network. In the thermophilic stage, Thermobifida was the exclusive degrader of cellulose, and Thermobifida fusca was the most important cellulolytic actinomycete. Saccharomonospora viridis, Planifilum fulgidum, Thermobacillus sp. and the dominant ascomycota of Aspergillus sclerotialis participated in hemicellulose decomposing. In the cooling phase, functional microorganisms became more diverse, with Nocardiopsis flavescens, Glycomyces artemisiae, Glycomyces sambucus, Streptomyces rubrolavendulae and Streptomyces vietnamensis joining the cellulose-degrading rank, and Chaetomium thermophilum emerging as the main hemicellulose degrader. More than two thirds of the bacteria-bacteria interactions and all the fungi-fungi associations were positive, while, both competition (for the same substrate of hemicellulose) and synergy (preference for cellulose and hemicellulose) coexisted in the bacteria-fungi interactions. In conclusion, these findings provide useful information for understanding the biodegradation of tomato plant residues better, and effects of the functional agents identified on composting process should be further studied.
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http://dx.doi.org/10.1016/j.jenvman.2021.113162DOI Listing
September 2021

Effects of Fermented Milk Containing Strain Shirota on Constipation in Patients with Depression: A Randomized, Double-Blind, Placebo-Controlled Trial.

Nutrients 2021 Jun 29;13(7). Epub 2021 Jun 29.

Department of Nutrition and Health, China Agricultural University, Beijing 100091, China.

Probiotics have been shown to benefit patients with constipation and depression, but whether they specifically alleviate constipation in patients with depression remains unclear. The aim of this study was to investigate the effect of strain Shirota (LcS), formerly strain Shirota, on constipation in patients with depression with specific etiology and gut microbiota and on depressive regimens. Eighty-two patients with constipation were recruited. The subjects consumed 100 mL of a LcS beverage (10 CFU/mL) or placebo every day for 9 weeks. After ingesting beverages for this period, we observed no significant differences in the total patient constipation-symptom (PAC-SYM) scores in the LcS group when compared with the placebo group. However, symptoms/scores in item 7 (rectal tearing or bleeding after a bowel movement) and items 8-12 (stool symptom subscale) were more alleviated in the LcS group than in the placebo group. The Beck Depression Index (BDI) and Hamilton Depression Rating Scale (HAMD) scores were all significantly decreased, and the degree of depression was significantly improved in both the placebo and LcS groups ( < 0.05), but there was no significant difference between the groups. The LcS intervention increased the beneficial , and levels and decreased the bacterial levels related to mental illness, such as , and Additionally, the interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) levels were significantly decreased in both the placebo and LcS groups ( < 0.05). In particular, the IL-6 levels were significantly lower in the LcS group than the placebo group after the ingestion period ( < 0.05). In conclusion, the daily consumption of LcS for 9 weeks appeared to relieve constipation and improve the potentially depressive symptoms in patients with depression and significantly decrease the IL-6 levels. In addition, the LcS supplementation also appeared to regulate the intestinal microbiota related to mental illness.
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http://dx.doi.org/10.3390/nu13072238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308326PMC
June 2021

Endogenous bacteria inhabiting the Ophiocordyceps highlandensis during fruiting body development.

BMC Microbiol 2021 Jun 11;21(1):178. Epub 2021 Jun 11.

Yunnan Herbal Laboratory, School of Ecology and Environmental Science, Yunnan University, Kunming, 650091, China.

Background: The genus Ophiocordyceps, which includes Ophiocordyceps sinensis, has been demonstrated to be one of the most valuable medicinal taxa. The low rate of larval infection and slow development that characterize the cultivation of this genus should be urgently addressed. To identify potential bioinoculants that stimulate the growth of Ophiocordyceps, O. highlandensis was selected as a model system, and a total of 72 samples were collected to systematically compare the microbial communities present during fruiting body development. By applying high-throughput 16S and ITS2 amplicon sequencing technology, the bacterial and fungal communities were identified in O. highlandensis and its surrounding soil, and the functional dynamics of the bacteria were explored.

Results: The results indicate that the most abundant bacteria across all the samples from O. highlandensis were Proteobacteria, Firmicutes and Bacteroidetes, while members of Ascomycota were detected among the fungi. The pathways enriched in the developmental stages were associated with carbohydrate degradation, nucleotides and pyridoxal biosynthesis, and the TCA cycle. Compared with that in the fungal community, an unexpectedly high taxonomic and functional fluctuation was discovered in the bacterial community during the maturation of O. highlandensis. Furthermore, bipartite network analysis identified four potential supercore OTUs associated with O. highlandensis growth.

Conclusions: All the findings of this study suggest unexpectedly high taxonomic and functional fluctuations in the bacterial community of O. highlandensis during its maturation. O. highlandensis may recruit different endogenous bacteria across its life cycle to enhance growth and support rapid infection. These results may facilitate Ophiocordyceps cultivation and improve the development of strategies for the identification of potential bioinoculant resources.
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http://dx.doi.org/10.1186/s12866-021-02227-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196446PMC
June 2021

Bipolar Aggregation-Induced Electrochemiluminescence of Thiophene-Fused Conjugated Microporous Polymers.

ACS Appl Mater Interfaces 2021 Jun 9;13(24):28782-28789. Epub 2021 Jun 9.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.

Herein, we synthesize the thiophene tetraphenylethene-based conjugated microporous polymer (ThT-CMP) using the tetraphenylethylene derivative [i.e., 1,1,2,2-tetrakis(4-bromophenyl)ethane (TPBE)] and 2,5-thiophenediboronic acid as the precursors. The aggregation of TPBE in the ThT-CMP can induce a strong dual-band bipolar electrochemiluminescence (AIECL) emission at 554 nm (anodic) and 559 nm (cathodic) with tri--propylamine (TPrA) and SO as the coreactants, respectively. The anodic and cathodic ECL efficiencies are measured to be 11.49 and 3.82% with respect to the standard of the Ru(bpy)/TPrA system, respectively. We further develop a dipolar ECL sensor to sensitively detect rhodamine B (RhB) based on resonance energy transfer. This ECL sensor possesses a large dynamic range and high sensitivity. This research provides a new avenue of designing organic structures with the characteristic of bipolar AIECL for the development of luminescent devices.
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http://dx.doi.org/10.1021/acsami.1c05195DOI Listing
June 2021

Potential biomarkers for inherited thrombocytopenia 2 identified by plasma proteomics.

Platelets 2021 Jun 8:1-8. Epub 2021 Jun 8.

Laboratory of Radiation Biology, Department of Blood Transfusion, Laboratory Medicine Center, The Second Affiliated Hospital, Army Medical University, Chongqing, China.

Inherited thrombocytopenia 2 (THC2) is difficult to diagnose due to the lack of specific clinical characteristics and diagnostic methods. To identify potential plasma protein biomarkers for THC2, we collected the plasma samples from a THC2 family (9 THC2 and 15 non-THC2 members), enriched the medium and low abundant proteins using Proteominer and analyzed the protein profiles using the liquid chromatography-mass spectrometry in data independent acquisition mode. Initially, we detected 784 proteins in the plasma samples of this family and identified 27 up-regulated and 36 down-regulated in the THC2 group compared to the non-THC2 group (|log ratio| >1 and -value <0.05). To improve the predictive power, top eight dysregulated proteins (B7Z2B4, LTF, HP, ERN1, IGHV1-8, A0A0X9V9C4, VH6DJ, and D3JV41) were selected by an area under the curve-based random forest process to construct a clinical model. Multivariate analysis with random forest and support vector machine showed that the prediction model provided high discrimination ability for THC2 diagnosis (AUC: 1.000 and 0.967, respectively). The potential plasma protein biomarkers will be tested in more THC2 patients and other thrombocytopenia patients to further validate their specificity and sensitivity.
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http://dx.doi.org/10.1080/09537104.2021.1937594DOI Listing
June 2021

Can the Non-native Salt Marsh Halophyte Threaten Native Seagrass () Habitats? A Case Study in the Yellow River Delta, China.

Front Plant Sci 2021 20;12:643425. Epub 2021 May 20.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Seagrass meadows are critical ecosystems, and they are among the most threatened habitats on the planet. As an anthropogenic biotic invader, Loisel. competes with native plants, threatens native ecosystems and coastal aquaculture, and may cause local biodiversity to decline. The distribution area of the exotic species in the Yellow River Delta had been expanding to ca.4,000 ha from 1990 to 2018. In this study, we reported, for the first time, the competitive effects of the exotic plant () on seagrass ( Asch. & Graebn.) by field investigation and a transplant experiment in the Yellow River Delta. Within the first 3 months of the field experiment, had pushed forward 14 m into the distribution region. In the study region, the area of in 2019 increased by 516 times compared with its initial area in 2015. Inhibition of growth increased with the invasion of . had been degrading significantly under the pressure of invasion. propagates sexually via seeds for long distance invasion and asexually by tillers and rhizomes for short distance invasion. Our results describe the invasion pattern of and can be used to develop strategies for prevention and control of invasion.
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http://dx.doi.org/10.3389/fpls.2021.643425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173042PMC
May 2021

Association Between Thyroid Hormone Levels and Advanced Liver Fibrosis in Patients with Type 2 Diabetes Mellitus and Non-Alcoholic Fatty Liver Disease.

Diabetes Metab Syndr Obes 2021 26;14:2399-2406. Epub 2021 May 26.

Department of Endocrinology, Beijing Jishuitan Hospital, Beijing, People's Republic of China.

Purpose: To evaluate the effects of serum thyroid hormone levels on advanced liver fibrosis in cases with NAFLD (non-alcoholic fatty liver disease) and T2DM (type 2 diabetes mellitus).

Patients And Methods: A total of 1422 cases with T2DM who were admitted to Peking University International Hospital between December 2014 and October 2019 were retrospectively analyzed. Standard anthropometry as well as clinical and laboratory evaluation were performed on all patients. Abdominal ultrasonography was performed to diagnose NAFLD. NFS (NAFLD fibrosis score) was used to identify advanced fibrosis in patients with T2DM and NAFLD.

Results: In cases with T2DM and NAFLD, the serum FT3 level in cases with advanced fibrosis was lower than that in those without advanced fibrosis (4.79±0.89 vs 4.28±1.19, < 0.05), and significant difference was not found in serum levels of FT4 and TSH between cases with advanced fibrosis and those without advanced fibrosis ( > 0.05). The incidence of advanced fibrosis declined as the rise of serum FT3 levels ( < 0.05). Besides, FT3 (OR, 0.492; 95% CI, 0.384-0.631) was noted as a factor influencing advanced fibrosis in cases with T2DM and NAFLD (< 0.05).

Conclusion: In cases with T2DM and NAFLD, the incidence of advanced fibrosis is negatively correlated with serum FT3 levels, and a low FT3 level is an independent risk factor of advanced fibrosis.
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http://dx.doi.org/10.2147/DMSO.S313503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165094PMC
May 2021

Investigation of the Multi-Target Mechanism of Guanxin-Shutong Capsule in Cerebrovascular Diseases: A Systems Pharmacology and Experimental Assessment.

Front Pharmacol 2021 13;12:650770. Epub 2021 May 13.

Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Guanxin-Shutong capsule (GXSTC), a combination of Mongolian medicines and traditional herbs, has been clinically proven to be effective in treating cerebrovascular diseases (CBVDs). However, the underlying pharmacological mechanisms of GXSTC in CBVDs remain largely unknown. In this study, a combination of systems pharmacology and experimental assessment approach was used to investigate the bioactive components, core targets, and possible mechanisms of GXSTC in the treatment of CBVDs. A total of 15 main components within GXSTC were identified using high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) and a literature research. Fifty-five common genes were obtained by matching 252 potential genes of GXSTC with 462 CBVD-related genes. Seven core components in GXSTC and 12 core genes of GXSTC on CBVDs were further determined using the protein-protein interaction (PPI) and component-target-pathway (C-T-P) network analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis results predicted that the molecular mechanisms of GXSTC on CBVDs were mainly associated with the regulation of the vascular endothelial function, inflammatory response, and neuronal apoptosis. Molecular docking results suggested that almost all of core component-targets have an excellent binding activity (affinity < -5 kcal/mol). More importantly, in middle cerebral artery occlusion (MCAO) -injured rats, GXSTC significantly improved the neurological function, reduced the infarct volume, and decreased the percentage of impaired neurons in a dose-dependent manner. Western blotting results indicated that GXSTC markedly upregulated the expression of vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS), while downregulating the expression of cyclooxygenase-2 (COX-2) and transcription factor AP-1 (c-Jun) in MCAO-injured rats. These findings confirmed our prediction that GXSTC exerts a multi-target synergetic mechanism in CBVDs by maintaining vascular endothelial function, inhibiting neuronal apoptosis and inflammatory processes. The results of this study may provide a theoretical basis for GXSTC research and the clinical application of GXSTC in CBVDs.
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http://dx.doi.org/10.3389/fphar.2021.650770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155632PMC
May 2021

Flower-like Hydroxyfluoride-Sensing Platform toward NO Detection.

ACS Appl Mater Interfaces 2021 Jun 28;13(22):26278-26287. Epub 2021 May 28.

Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education and School of Materials Science and Engineering, Shandong University, Jinan, Shandong 250061, China.

We report for the first time using zinc hydroxyfluoride (ZnOHF) for efficient NO gas detection. The prepared ZnOHF had a unique flower-like architecture self-assembled by nanorods with a diameter of 150 nm and length of 2-3 μm. The sensing performance toward NO detection indicated that the prepared ZnOHF exhibited high response (82.71), short response/recovery time (13 s/35 s) to 10 ppm of NO, and excellent selectivity at 200 °C, greatly outperforming the ZnO raw material. ZnOHF could work in a wide detection window ranging from 100 ppb to 50 ppm, implying its practical application prospects in both industry and daily life. The excellent sensing behavior of ZnOHF originated mainly from the negligible oxygen ions adsorbed on the material surface, which was caused by the higher work function of ZnOHF. Therefore, almost all conduction band electrons can be used in the NO gas sensing.
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http://dx.doi.org/10.1021/acsami.1c02176DOI Listing
June 2021

Antinociceptive effects of macrophage-derived extracellular vesicles by carrying microRNA-216a.

Am J Transl Res 2021 15;13(4):1971-1989. Epub 2021 Apr 15.

Department of Anatomy, Kunming Medical University Haiyuan College Kunming 650101, Yunnan, P. R. China.

Cancer-induced bone pain (CIBP) represents the pain induced by bone metastases from malignancies. The role of extracellular vesicles (Evs) has been underscored in bone metastasis. However, the function of Evs, especially these derived from M2 macrophages (M2φ-Evs) in CIBP is unclear. Therefore, this investigation aimed to probe the possible antinociceptive effect of M2φ-Evs in CIBP and the underlying mechanism of action. Using the C57bl/6 mice, a CIBP animal model was established by the administration of Walker 256 mammary gland carcinoma cells, followed by M2φ-Evs administration. It was found that CIBP mice treated with M2φ-Evs had significantly reduced nociception and serum inflammatory factors. Microarray sequencing revealed that microRNA-216a (miR-216a) was the most upregulated miRNA in Evs-treated mouse spinal cord tissues. Subsequent bioinformatics, GSEA and KEGG enrichment analyses demonstrated that HMGB1 and TLR4-NF-κB pathway were the downstream effectors of miR-216a and were both downregulated in spinal cord tissues of CIBP mice treated with M2φ-Evs. Rescue experiments displayed that after we reduced miR-216a expression in M2φ-Evs, the antinociceptive effect of M2φ-Evs on CIBP mice was inhibited, and the HMGB1 expression and the TLR4-NF-κB signaling were significantly activated. Together, M2φ-Evs relieve CIBP by carrying miR-216a, which was elicited through the HMGB1/TLR4-NF-κB axis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129326PMC
April 2021

Relationships Between Annual and Perennial Seagrass () Populations and Their Sediment Geochemical Characteristics in the Yellow River Delta.

Front Plant Sci 2021 20;12:634199. Epub 2021 Apr 20.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Annual and perennial populations commonly occur for the same submerged aquatic angiosperm species, yet relationships between population types and sediment characteristics are poorly understood. In the current study two habitats with annual and perennial populations were surveyed in the Yellow River Delta (YRD). Biomass and seasonal seed bank size were used to evaluate population status and potential recruitment capacity. Sediment geochemical parameters including moisture, sulfide, Chl , carbohydrate, OM, TOC, TN, and TP were measured to compare sediment nutrient composition and variability. The results revealed a higher biomass and larger seed bank in the annual population compared with the perennial population. The P levels in sediments between the two populations were similar; while the N level in the sediment of the annual population was significantly higher than the perennial population, which might support the recruitment of vegetative shoots when a large amount of seeds germinated during wet periods. The annual population exhibited greater resilience after habitat desiccation, with the population recovering rapidly once water appeared. The results of this study add to the knowledge of populations and their sediment geochemical characteristics, and can be used as a reference for population conservation and management.
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http://dx.doi.org/10.3389/fpls.2021.634199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8095395PMC
April 2021

An enantioselective aza-Friedel-Crafts reaction of 5-aminoisoxazoles with isatin-derived -Boc ketimines.

Org Biomol Chem 2021 May;19(17):3820-3824

Asymmetric Synthesis and Chiraltechnology Key Laboratory of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, China. and Department of Chemistry, Xihua University, China.

By employing a chiral phosphoric acid as a catalyst, an enantioselective aza-Friedel-Crafts reaction of 5-aminoisoxazoles with isatin-derived N-Boc ketimines was realized. The reaction provided a wide variety of novel 3-isoxazole 3-amino-oxindoles with good yields (up to 99%) and moderate to good enantioselectivities (up to 99%). The absolute configuration of one product was assigned by X-ray crystal structural analysis and a plausible reaction mechanism was proposed. In addition, a scale-up reaction was performed successfully. Finally, one product was subjected to Suzuki-Miyaura coupling with phenylboronic acid to afford the product in a moderate yield without erosion of the enantioselectivity.
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http://dx.doi.org/10.1039/d1ob00374gDOI Listing
May 2021

Predictive Value of First-Trimester Glycosylated Hemoglobin Levels in Gestational Diabetes Mellitus: A Chinese Population Cohort Study.

J Diabetes Res 2021 9;2021:5537110. Epub 2021 Apr 9.

Department of Endocrinology and Metabolism, Peking University International Hospital, Beijing 102206, China.

This study was aimed at exploring the predictive value of first-trimester glycosylated hemoglobin (HbA1c) levels in the diagnosis of gestational diabetes mellitus (GDM). A total of 744 pregnant women registered at the Peking University International Hospital between March 2017 and March 2019 were included in this study. Data on personal characteristics and biochemical indicators of the pregnant women were collected during the first trimester. The International Association of Diabetes and Pregnancy Study Groups has adopted specific diagnostic criteria as the gold standard for the diagnosis of GDM. Receiver operating characteristic (ROC) curve statistics were used to assess the predictive value of first-trimester HbA1c levels in the diagnosis of GDM. HbA1c levels in the first trimester were significantly higher in the GDM group than in the non-GDM group (5.23% ± 0.29% vs. 5.06 ± 0.28%, < 0.05). The first-trimester HbA1c level was an independent risk factor for gestational diabetes. The area under the ROC curve (AUC) of HbA1c for GDM was 0.655 (95% confidence interval 0.620-0.689, < 0.001). The positive likelihood ratio was the highest at HbA1c = 5.9%, sensitivity was 2.78, and specificity was 99.83%. There was no statistical difference in AUC between fasting blood glucose and HbA1c ( = 0.407). First-trimester HbA1c levels can be used to predict GDM. The risk of GDM was significantly increased in pregnant women with first-trimester HbA1c levels > 5.9%. There was no statistical difference between first-trimester HbA1c and fasting blood glucose levels in predicting GDM.
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http://dx.doi.org/10.1155/2021/5537110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053049PMC
April 2021

Cross-Substitution Promoted Ultrawide Bandgap up to 4.5 eV in a 2D Semiconductor: Gallium Thiophosphate.

Adv Mater 2021 Jun 19;33(22):e2008761. Epub 2021 Apr 19.

State Key Laboratory of Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences & Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100083, China.

Exploring 2D ultrawide bandgap semiconductors (UWBSs) will be conductive to the development of next-generation nanodevices, such as deep-ultraviolet photodetectors, single-photon emitters, and high-power flexible electronic devices. However, a gap still remains between the theoretical prediction of novel 2D UWBSs and the experimental realization of the corresponding materials. The cross-substitution process is an effective way to construct novel semiconductors with the favorable parent characteristics (e.g., structure) and the better physicochemical properties (e.g., bandgap). Herein, a simple case is offered for rational design and syntheses of 2D UWBS GaPS by employing state-of-the-art GeS as a similar structural model. Benefiting from the cosubstitution of Ge with lighter Ga and P, the GaPS crystals exhibit sharply enlarged optical bandgaps (few-layer: 3.94 eV and monolayer: 4.50 eV) and superior detection performances with high responsivity (4.89 A W ), high detectivity (1.98 × 10 Jones), and high quantum efficiency (2.39 × 10 %) in the solar-blind ultraviolet region. Moreover, the GaPS -based photodetector exhibits polarization-sensitive photoresponse with a linear dichroic ratio of 1.85 at 254 nm, benefitting from its in-plane structural anisotropy. These results provide a pathway for the discovery and fabrication of 2D UWBS anisotropic materials, which become promising candidates for future solar-blind ultraviolet and polarization-sensitive sensors.
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http://dx.doi.org/10.1002/adma.202008761DOI Listing
June 2021

Prevention of deep venous thrombosis in stroke: a best practice implementation project.

JBI Evid Implement 2021 Apr 9. Epub 2021 Apr 9.

Neurology Department, Nanfang Hospital, Southern Medical University The Nanfang Nursing Centre for Evidence-based Practice: A JBI Centre of Excellence Nursing Department, Nanfang Hospital, Southern Medical University, Guangzhou, PR China.

Objectives: The current evidence implementation project aims to promote evidence-based practice in the care of patients with stroke, specifically around the prevention of deep venous thrombosis (DVT), in the neurology department of a tertiary hospital in Guangzhou, China.

Introduction: The prevalence of DVT within 14 days after stroke is 10-75%. Approximately 20% of patients with DVT develop pulmonary embolism, the third most common cause of death in patients with stroke and the most common cause of autopsy-verified death between the second and fourth week after stroke. Several risk factors are associated with the development of DVT, many of which can be alleviated by evidence-based strategies that can prevent or reduce the risk of DVT.

Methods: The current evidence implementation project utilized the Joanna Briggs Institute Practical Application of Clinical Evidence System program and involved 30 patients in each audit criterion. Interventions included staff education, involvement of rehabilitation staff, and building a patient education bundle. A postimplementation reaudit was undertaken including the same number of patient samples.

Results: The baseline audit results showed that the compliances for two audit criteria (patient mobilization and patient education) were under 20%, whereas those for the other criteria were at least 60%. After implementing the strategies, there were significant improvements especially in the two weak-compliance criteria. In addition, the overall compliance for most criteria increased.

Conclusion: The project standardized the DVT prevention process in the Neurology Department of Nanfang Hospital. The overall compliance with DVT prevention for patients with stroke in the department improved.
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http://dx.doi.org/10.1097/XEB.0000000000000278DOI Listing
April 2021

The Exploration of Novel Pharmacophore Characteristics and Multidirectional Elucidation of Structure-Activity Relationship and Mechanism of Sesquiterpene Pyridine Alkaloids from Based on Computational Approaches.

Evid Based Complement Alternat Med 2021 24;2021:6676470. Epub 2021 Mar 24.

Chongqing Academy of Chinese Materia Medica, No. 34 Nanshan Road, Nan'an District, Chongqing 400065, China.

Sesquiterpene pyridine alkaloids are a large group of highly oxygenated sesquiterpenoids, which are characterized by a macrocyclic dilactone skeleton containing 2-(carboxyalkyl) nicotinic acid and dihydro--agarofuran sesquiterpenoid, and are believed to be the active and less toxic components of . In this study, 55 sesquiterpene pyridine alkaloids from were subjected to identification of pharmacophore characteristics and potential targets analysis. Our results revealed that the greatest structural difference of these compounds was in the pyridine ring and the pharmacophore model-5 (Pm-05) was the best model that consisted of three features including hydrogen bond acceptor (HBA), hydrogen bond donor (HBD), and hydrophobic (HY), especially hydrophobic group located in the pyridine ring. It was proposed that 2-(carboxyalkyl) nicotinic acid part possessing a pyridine ring system was not only a pharmacologically active center but also a core of structural diversity of alkaloids from . Furthermore, sesquiterpene pyridine alkaloids from were predicted to target multiple proteins and pathways and possibly played essential roles in the cure of Alzheimer's disease, breast cancer, Chagas disease, and nonalcoholic fatty liver disease (NAFLD). They also had other pharmacological effects, depending on the binding interactions between pyridine rings of these compounds and active cavities of the target genes platelet-activating factor receptor (PTAFR), cannabinoid receptor 1 (CNR1), cannabinoid receptor 1 (CNR2), squalene synthase (FDFT1), and heat shock protein HSP 90-alpha (HSP90AA1). Taken together, the results of this present study indicated that sesquiterpene pyridine alkaloids from are promising candidates that exhibit potential for development as medicine sources and need to be promoted.
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http://dx.doi.org/10.1155/2021/6676470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012133PMC
March 2021

Efficacy and Safety of Mulberry Twig Alkaloids Tablet for the Treatment of Type 2 Diabetes: A Multicenter, Randomized, Double-Blind, Double-Dummy, and Parallel Controlled Clinical Trial.

Diabetes Care 2021 Apr 8. Epub 2021 Apr 8.

Department of Endocrinology, West China Hospital, Sichuan University, Sichuan, China.

Objective: This study aimed to evaluate the efficacy and safety of mulberry twig alkaloids (Sangzhi alkaloids [SZ-A]) in the treatment of type 2 diabetes (T2D).

Research Design And Methods: This was a multicenter, randomized, double-blind, double-dummy, and parallel controlled noninferiority clinical trial that was conducted for 24 weeks. A total of 600 patients were randomly allocated to the SZ-A group ( 360) or acarbose group ( 240). The primary efficacy end point was the change of glycosylated hemoglobin (HbA) compared with baseline. In addition, adverse events (AEs), severe AEs (SAEs), treatment-related AEs (TAEs), and gastrointestinal disorders (GDs) were monitored.

Results: After treatment for 24 weeks, the change in HbA was -0.93% (95% CI -1.03 to -0.83) (-10.2 mmol/mol [-11.3 to -9.1]) and -0.87% (-0.99 to -0.76) (-9.5 mmol/mol [-10.8 to -8.3]) in the SZ-A and acarbose groups, respectively, and the least squares mean difference was -0.05% (95% CI -0.18 to 0.07) (-0.5 mmol/mol [-2.0 to 0.8]) between the two groups, with no significant difference on the basis of covariance analysis ( > 0.05). The incidence of TAEs and GDs was significantly lower in the SZ-A group than the acarbose group ( < 0.01), but no differences for AEs or SAEs between the two groups were observed ( > 0.05).

Conclusions: SZ-A exhibited equivalent hypoglycemic effects to acarbose in patients with T2D. Nevertheless, the incidence of TAEs and GDs was lower following SZ-A treatment than acarbose treatment, suggesting good safety.
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http://dx.doi.org/10.2337/dc20-2109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247493PMC
April 2021

Diversity, distribution and conservation of seagrass in coastal waters of the Liaodong Peninsula, North Yellow Sea, northern China: Implications for seagrass conservation.

Mar Pollut Bull 2021 Jun 31;167:112261. Epub 2021 Mar 31.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Seagrass beds are highly productive coastal ecosystems that are widely distributed along temperate and tropical coastlines globally. Although seagrass distribution and diversity have been widely reported on a global scale, there have been few reports on seagrass distribution and diversity in northern China, especially for coastal waters of the Liaodong Peninsula in the North Yellow Sea. In the present study, we investigated the distribution and diversity of seagrass in coastal waters of the Liaodong Peninsula in the North Yellow Sea, northern China. Field surveys of seagrass wrack were conducted along shorelines, to identify whether seagrass beds occurred in nearby waters, and sonar methods were then used to collect data relating to seagrass bed extent. Also, we analyzed the major threats facing seagrass beds. The results of the study revealed that four species (Zostera marina L., Z. japonica Aschers. & Graebn., Z. caespitosa M., and Phyllospadix iwatensis M.) were found in study area, covering a total area of 1253.47 ha. Seagrass bed area significantly decreased with increasing water depth, and most seagrass was recorded at depths of 2-5 m. Due to the steep slope of the seabed, seagrass beds exhibited a zonal distribution in most of the study areas. In addition, the amount of seagrass wrack along shorelines could be used to infer the size and distance of seagrass beds. Human activities, such as clam harvesting, land reclamation, coastal aquaculture pose a threat to the seagrass beds. This study provides new information to fill knowledge gaps regarding seagrass distribution in northern China and it provides a baseline for further monitoring of these seagrass beds.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112261DOI Listing
June 2021

Salivary Microbiota for Gastric Cancer Prediction: An Exploratory Study.

Front Cell Infect Microbiol 2021 10;11:640309. Epub 2021 Mar 10.

Department of Gastroenterology and Hepatology, The First Medical Center, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.

To characterize the salivary microbiota in patients at different progressive histological stages of gastric carcinogenesis and identify microbial markers for detecting gastric cancer, two hundred and ninety-three patients were grouped into superficial gastritis (SG; n = 101), atrophic gastritis (AG; n = 93), and gastric cancer (GC; n = 99) according to their histology. 16S rRNA gene sequencing was used to access the salivary microbiota profile. A random forest model was constructed to classify gastric histological types based on the salivary microbiota compositions. A distinct salivary microbiota was observed in patients with GC when comparing with SG and AG, which was featured by an enrichment of putative proinflammatory taxa including and . Among the significantly decreased oral bacteria in GC patients including , , , , , and , , and are known to reduce nitrite, which may consequently result in an accumulation of carcinogenic N-nitroso compounds. We found that GC can be distinguished accurately from patients with AG and SG (AUC = 0.91) by the random forest model based on the salivary microbiota profiles, and taxa belonging to and have potential as diagnostic biomarkers for GC. Remarkable changes in the salivary microbiota functions were also detected across three histological types, and the upregulation in the isoleucine and valine is in line with a higher level of these amino acids in the gastric tumor tissues that reported by other independent studies. Conclusively, bacteria in the oral cavity may contribute gastric cancer and become new diagnostic biomarkers for GC, but further evaluation against independent clinical cohorts is required. The potential mechanisms of salivary microbiota in participating the pathogenesis of GC may include an accumulation of proinflammatory bacteria and a decline in those reducing carcinogenic N-nitroso compounds.
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http://dx.doi.org/10.3389/fcimb.2021.640309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7988213PMC
July 2021

New insights into physiological effects of anoxia under darkness on the iconic seagrass Zostera marina based on a combined analysis of transcriptomics and metabolomics.

Sci Total Environ 2021 May 21;768:144717. Epub 2021 Jan 21.

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China; CAS Engineering Laboratory for Marine Ranching, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Coastal hypoxia/anoxia is a major emerging threat to global coastal ecosystems. Macroalgae blooms of tens of kilometers are often observed in open waters. These blooms not only cause a lack of oxygen, but also benthic light limitation. We explored the physiological responses of Zostera marina L. to anoxia under darkness. After exposing Z. marina to anoxia under darkness for 72 h, we measured the elongation of leaves and the decrease in maximal quantum yield of photosystem II (Fv/Fm), and investigated the transcriptomic and metabolomic responses to anoxic stress based on RNA-sequencing and liquid chromatography-mass spectrometry (LC-MS) technology. The results showed that anoxic stress significantly reduced the leaf Fv/Fm, and had a significant negative effect on the photosynthesis and growth of Z. marina. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of up-regulated differentially expressed genes (DEGs) showed that glycolysis was the most significant enrichment pathway (p < 0.001), and most of the important products in glycolysis were significantly up-regulated. This indicated that the glycolysis process of anaerobic respiration is promoted under anoxia. The metabolite results also showed that glyceraldehyde 3-phosphate in the glycolysis pathway was significantly up-regulated. Moreover, three genes encoding sucrose synthase (gene-ZOSMA_310G00150, gene-ZOSMA_81G00980, and gene-ZOSMA_8G00730) and one gene encoding alpha-amylase (gene-ZOSMA_95G00270) were significantly up-regulated, providing the sugar basis for the subsequent increase in glycolysis. Furthermore, gene-encoding oxoglutarate dehydrogenase, the rate-limiting step of the tricarboxylic acid (TCA) cycle, was significantly down-regulated, indicating that this cycle was inhibited under anoxia. Metabolomic results showed that L-tryptophan, L-phenylalanine, and DL-leucine were significantly up-regulated. Only significantly decreased glutamate and non-significantly decreased glutamine, substances consumed in alanine and γ-aminobutyric acid (GABA) shunt mechanisms, were detected in the leaves, while GABA and alanine were not detected. The results of this study show that anoxic stress induces a programmed transcriptomic and metabolomic response in seagrass, most likely reflecting a complex strategy of acclimation and adaptation in seagrass to resist anoxic stress.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144717DOI Listing
May 2021

Beverages containing Lactobacillus paracasei LC-37 improved functional dyspepsia through regulation of the intestinal microbiota and their metabolites.

J Dairy Sci 2021 Jun 11;104(6):6389-6398. Epub 2021 Mar 11.

Department of Nutrition and Health, China Agricultural University, Beijing 100083, China. Electronic address:

Functional dyspepsia (FD) is a common disease of the digestive system and probiotics have been reported to be effective in the treatment of functional gastrointestinal diseases. The aim of this study was to determine the effect of the beverage containing Lactobacillus paracasei LC-37 (LC-37) and its ability to relieve symptoms of FD by a randomized clinical trial. Due to the mechanistic complexity underlying FD, intestinal microbiota and stool metabolomes were also evaluated. The results showed that FD was relieved in participants after treatment with the beverage containing LC-37 for 14 and 28 d. The clinical symptom scores were significantly decreased after these times (abdominal pain and belching were significantly decreased after 14 d and almost absent after 28 d of treatment). Probiotics, such as those containing the Lactobacillus, Lactococcus, and Weissella, significantly increased, and the abundance of harmful bacteria such as Lachnocliostridium significantly decreased. Furthermore, relevant beneficial intestinal metabolites such as pelargonic acid, benzoic acid, and short-chain fatty acids increased, and harmful intestinal metabolites such as hippuric acid decreased. Taken together, these findings suggested that the beverages containing LC-37 can increase the abundance of probiotics and decrease pathogenic bacteria, and thereby improve beneficial intestinal metabolites. Therefore, the beverages containing LC-37 may provide a natural alternative to combat FD.
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http://dx.doi.org/10.3168/jds.2020-19882DOI Listing
June 2021

The effect of different inflating volume on the measurement accuracy of the modified cuff pressure measurement method.

J Clin Monit Comput 2021 Mar 11. Epub 2021 Mar 11.

Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

To evaluate the effect of different inflation volume on the measurement accuracy of the modified cuff pressure measurement method in different shapes of cuffs, so as to provide reference for the correct monitoring of cuff pressure in clinic. In vitro study: The traditional cuff pressure measurement method (the cuff pressure gauge before measurement shows 0 cm HO) and the modified cuff pressure measurement method (the cuff pressure before measurement shows 25 cm HO, 28 cm HO, 30 cm HO or 32 cm HO) were used to measure cylindrical and tapered cuffs, and the effect of different inflation volume on cuff pressure was analyzed statistically. Clinical study: patients with the artificial airway established by orotracheal intubation or tracheotomy in Neuro-ICU were prospectively selected as subjects, and the measurement procedure was the same as in vitro study. In vitro study showed that the pressure loss values of cylindrical cuff and tapered cuff using the traditional cuff pressure measurement method were (3.75 ± 0.31) cm HO and (4.92 ± 0.44) cm HO, respectively, and clinical study showed that the pressure loss values were (5.07 ± 0.83) cm HO and (5.17 ± 0.93) cm HO, respectively. The actual measured values measured by the traditional cuff pressure measurement method of the two cuff shapes were compared with the corrected target value of 28 cm HO, and the differences were statistically significant (P < 0.000). Both in vitro and clinical study had shown that all differences between the actual measured value and the corrected target value using the modified cuff pressure measurement method (measured with 25 cm HO, 30 cm HO, 32 cm HO) were statistically significant (P < 0.000), and the range of overall differences was (0-1.23 ± 0.25) cm HO. In vitro study had shown that the pressure variation coefficient (CV) of the tapered cuff was greater than that of the cylindrical cuff, and the difference was statistically significant (3.08 ± 0.25 VS 2.41 ± 0.21, P < 0.000). The traditional cuff pressure measurement method can directly lead to the cuff pressure drop, which is easy to cause the leakage of secretions on the cuffs and the misjudgment of the cuff pressure by medical personnel. However, the modified cuff pressure measurement method can effectively reduce cuff pressure loss, and taking the actual cuff pressure value as the inflation volume is the highest measurement accuracy.The tapered cuff is more susceptible to air volume, so it is necessary to pay attention to its measurement and correction in clinical practice.
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http://dx.doi.org/10.1007/s10877-021-00681-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7951126PMC
March 2021

The clinical features, management, and survival of elderly patients with colorectal cancer.

J Gastrointest Oncol 2021 Feb;12(1):89-99

Department of Gastroenterology, the First Medical Center of PLA General Hospital, Beijing, China.

Background: As the population ages, the number of elderly patients with colorectal cancer is increasing year by year. However, older people have rarely been the focus of studies on colorectal cancer. Therefore, in the present study, we aimed to carry out a retrospective analysis of this patient subgroup.

Methods: A retrospective study of clinical data of patients aged over 80 years who died from colorectal cancer in our hospital between 1993 and 2020 was performed. Logistic regression, the Kaplan-Meier method, and a multivariate Cox proportional hazards model were used to analyze the overall survival and treatment outcomes of the patients.

Results: A total of 87 patients were included in the study. The overall median survival was 45 months. In most patients, the primary lesion was located in the right colon. One-quarter of the patients refused to accept any treatment. Patients with stage IV tumors, who accounted for the largest proportion of the study population, displayed a higher rate of abandoning treatment than did patients of other stages. Almost all patients with stages II and III accepted surgery. Patients who underwent surgery to treat their colorectal cancer had longer survival than those who did not.

Conclusions: Old age should not be a reason for giving up treatment for colorectal cancer. The treatment of colorectal cancer patients aged 80 years and above requires individualized evaluation and more aggressive treatment to achieve greater benefits.
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http://dx.doi.org/10.21037/jgo-21-63DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944162PMC
February 2021
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