Publications by authors named "Xiaolong Zhao"

117 Publications

Operative choice for subtrochanteric femoral fracture in school-aged children: Triple elastic stable intramedullary nail versus locking plate.

Front Pediatr 2022 26;10:894262. Epub 2022 Jul 26.

Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The management strategy of subtrochanteric fractures remains controversial, and triple elastic stable intramedullary nail (ESIN) has not been reported for pediatric subtrochanteric fractures. This study aimed to compare the clinical effects of treating school-aged children with subtrochanteric fractures with triple ESINs versus locking plates.

Methods: We conducted a retrospective review of pediatric patients with subtrochanteric femoral fracture receiving either triple ESINs (TE) or locking plates (LPs) between January 2010 and January 2018. Sixteen patients in each group with matched age, sex, and fracture characteristics were included in the study. The preoperative data, including baseline information of the patients, fracture pattern, and types of surgical procedure, were collected from the hospital database. Patients were followed-up at the outpatient clinic in the 3rd month, 6th month, 12th month, and annually afterward. Hardware removal was performed at 9 - 18 months after the primary surgery.

Results: In all, 16 patients (8.4 ± 1.5-year-old, 7 boys, 9 girls) in the TE group and 16 patients (8.4 ± 1.4-year-old, 7 boys, 9 girls) in the LP group were included. There was significantly less operative time, reduced estimated blood loss, and shortened hospital stay for the TE as compared with the LP ( < 0.001). However, higher fluoroscopy frequency was observed in the TE group than in the LP group ( < 0.001). The time to union was faster in the TE group than in the LP group (P = 0.031). However, the angulation was higher in the TE group (3.2 ± 0.6) than the LP group (1.8 ± 0.5), and the incidence of implant prominence was higher in the TE group (7/16, 43.8%) than the LP group (1/16, 6.3%).

Conclusion: Compared with the locking plates, triple ESINs demonstrated significantly less operative time, reduced estimated blood loss, and shortened hospital stay. Besides, both TE and LP groups produced satisfactory outcomes in school-aged children with subtrochanteric fractures. Therefore, TE remains a feasible choice for subtrochanteric fractures in school-aged children.
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http://dx.doi.org/10.3389/fped.2022.894262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360405PMC
July 2022

Preparation and Characterization of Screen-Printed CuS/PEDOT:PSS Hybrid Films for Flexible Thermoelectric Power Generator.

Nanomaterials (Basel) 2022 Jul 15;12(14). Epub 2022 Jul 15.

School of Electrical and Control Engineering, North University of China, Taiyuan 030051, China.

In recent years, flexible thermoelectric generators(f-TEG), which can generate electricity by environmental temperature difference and have low cost, have been widely concerned in self-powered energy devices for underground pipe network monitoring. This paper studied the CuS films by screen-printing. The effects of different proportions of p-type CuS/poly 3,4-ethylene dioxythiophene-polystyrene sulfonate (PEDOT:PSS) mixture on the thermoelectric properties of films were studied. The interfacial effect of the two materials, forming a superconducting layer on the surface of CuS, leads to the enhancement of film conductivity with the increase of PEDOT:PSS. In addition, the Seebeck coefficient decreases with the increase of PEDOT:PSS due to the excessive bandgap difference between the two materials. When the content ratio of CuS and PEDOT:PSS was 1:1.2, the prepared film had the optimal thermoelectric performance, with a maximum power factor (PF) of 20.60 μW·m·K. The conductivity reached 75% of the initial value after 1500 bending tests. In addition, a fully printed Te-free f-TEG with a fan-shaped structure by CuS and AgSe was constructed. When the temperature difference (ΔT) was 35 K, the output voltage of the f-TEG was 33.50 mV, and the maximum power was 163.20 nW. Thus, it is envisaged that large thermoelectric output can be obtained by building a multi-layer stacking f-TEG for continuous self-powered monitoring.
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http://dx.doi.org/10.3390/nano12142430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324872PMC
July 2022

APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b.

Cell Mol Life Sci 2022 Jul 25;79(8):446. Epub 2022 Jul 25.

Laboratory of Molecular Biology and DNA Repair, Department of Medicine, University of Udine, Udine, Italy.

Increasing evidence suggests different, not completely understood roles of microRNA biogenesis in the development and progression of lung cancer. The overexpression of the DNA repair protein apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) is an important cause of poor chemotherapeutic response in lung cancer and its involvement in onco-miRNAs biogenesis has been recently described. Whether APE1 regulates miRNAs acting as prognostic biomarkers of lung cancer has not been investigated, yet. In this study, we analyzed miRNAs differential expression upon APE1 depletion in the A549 lung cancer cell line using high-throughput methods. We defined a signature of 13 miRNAs that strongly correlate with APE1 expression in human lung cancer: miR-1246, miR-4488, miR-24, miR-183, miR-660, miR-130b, miR-543, miR-200c, miR-376c, miR-218, miR-146a, miR-92b and miR-33a. Functional enrichment analysis of this signature revealed its biological relevance in cancer cell proliferation and survival. We validated DICER1 as a direct functional target of the APE1-regulated miRNA-33a-5p and miR-130b-3p. Importantly, IHC analyses of different human tumors confirmed a negative correlation existing between APE1 and Dicer1 protein levels. DICER1 downregulation represents a prognostic marker of cancer development but the mechanisms at the basis of this phenomenon are still completely unknown. Our findings, suggesting that APE1 modulates DICER1 expression via miR-33a and miR-130b, reveal new mechanistic insights on DICER1 regulation, which are of relevance in lung cancer chemoresistance and cancer invasiveness.
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http://dx.doi.org/10.1007/s00018-022-04443-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314295PMC
July 2022

Amine-Modified Chitosan Flocculant Synthesized via Single-Mode Microwave Method for Laundry Wastewater Treatment.

ACS Omega 2022 Jul 5;7(28):24522-24530. Epub 2022 Jul 5.

Liaoning Petrochemical University, Dandong Road, Wanghua District, Fushun City, Liaoning Province 113001, China.

In this study, an effective and environmentally friendly polyaminated cross-linked chitosan (M-PACTS) flocculant was successfully synthesized via circular focus single-mode microwave synthesizer irradiation. Epichlorohydrin and tetraethylenepentamine were used as the cross-linking agent and active cationic reagent, respectively. The same formation was used to prepare cationic lightly cross-linked chitosan (C-PACTS) via the conventional heating method. The flocculant was characterized using Fourier transform infrared spectroscopy, X-ray diffraction analysis, and scanning electron microscopy. The flocculation capability of C-PACTS and M-PACTS was compared using laundry wastewater as a model pollutant. The pH, PACTS dosage, temperature, stirring rate, stirring time, and setting time were systematically investigated. The experimental results showed that circular focus single-mode microwave synthesizer irradiation was a more efficient method to modify chitosan. M-PACTS exhibited a higher capacity for turbidity and chemical oxygen demand (COD) removal. Under optimal conditions, the removal rate values of M-PACTS were up to 96% (turbidity) and 78% (COD). The proposed PACTS is suitable for treatment of polluted wastewater in an eco-friendly manner without causing secondary pollution.
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http://dx.doi.org/10.1021/acsomega.2c02189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301697PMC
July 2022

Perthes Disease in a Child With Osteogenesis Imperfecta From a Rare Genetic Variant: A Case Report.

Front Genet 2022 8;13:920950. Epub 2022 Jul 8.

Department of Orthopaedic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Although certain genetic components have been reported as contributing factors for Perthes disease, its etiology remains unclear. We present a rare case of Perthes disease in a child with osteogenesis imperfecta (OI) caused by a mutation in the gene . A 7-year-old boy was initially treated at our medical facility in March 2016 with a history of chronic pain in right hip joint and limping for a year. He was diagnosed as Perthes disease in the right hip joint. He underwent acetabular osteotomy and ipsilateral proximal femoral varus osteotomy for better containment. During the follow-ups, the right hip demonstrated a normal range of motion without pain, and the pelvic X-ray demonstrated Stulberg Type II hip joint with a round femoral head. In the latest admission in 2022, he suffered from a right femoral shaft fracture after petty violence. After reviewing his medical history, he was suspected of having OI. The whole exome sequencing demonstrated a gene mutation in (OMIM 166200) and confirmed the diagnosis of OI. Telescopic nailing was used to treat the femoral shaft fracture. After the nailing of the right femur, the appearance of the lower extremity seemed normal and symmetrical. This study revealed that there might be an association between OI and Perthes disease. Our case report enriches the phenotypes of osteogenesis imperfecta and provides insight into the pathogenesis of LCPD.
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http://dx.doi.org/10.3389/fgene.2022.920950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304686PMC
July 2022

The Development of Microscopic Imaging Technology and its Application in Micro- and Nanotechnology.

Front Chem 2022 5;10:931169. Epub 2022 Jul 5.

Laboratory of Optical Detection and Imaging, School of Science, Qingdao University of Technology, Qingdao, China.

As a typical microscopic imaging technology, the emergence of the microscope has accelerated the pace of human exploration of the micro world. With the development of science and technology, microscopes have developed from the optical microscopes at the time of their invention to electron microscopes and even atomic force microscopes. The resolution has steadily improved, allowing humans to expand the field of research from the initial animal and plant tissues to microorganisms such as bacteria, and even down to the nanolevel. The microscope is now widely used in life science, material science, geological research, and other fields. It can be said that the development of microscopes also promotes the development of micro- and nanotechnology. It is foreseeable that microscopes will play a significant part in the exploration of the microworld for a long time to come. The development of microscope technology is the focus of this study, which summarized the properties of numerous microscopes and discussed their applications in micro and nanotechnology. At the same time, the application of microscopic imaging technology in micro- and nanofields was investigated based on the properties of various microscopes.
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http://dx.doi.org/10.3389/fchem.2022.931169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294601PMC
July 2022

The Development and Progression of Micro-Nano Optics.

Front Chem 2022 20;10:916553. Epub 2022 Jun 20.

Laboratory of Optical Detection and Imaging, School of Science, Qingdao University of Technology, Qingdao, China.

Micro-Nano optics is one of the most active frontiers in the current development of optics. It combines the cutting-edge achievements of photonics and nanotechnology, which can realize many brand-new functions on the basis of local electromagnetic interactions and become an indispensable key science and technology of the 21st century. Micro-Nano optics is also an important development direction of the new optoelectronics industry at present. It plays an irreplaceable role in optical communication, optical interconnection, optical storage, sensing imaging, sensing measurement, display, solid-state lighting, biomedicine, security, green energy, and other fields. In this paper, we will summarize the research status of micro-nano optics, and analyze it from four aspects: micro-nano luminescent materials and devices, micro-nano optical waveguide materials and devices, micro-nano photoelectric detection materials and devices, and micro-nano optical structures and devices. Finally, the future development of micro-nano optics will be prospected.
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http://dx.doi.org/10.3389/fchem.2022.916553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251314PMC
June 2022

Liddle syndrome misdiagnosed as primary aldosteronism is caused by inaccurate aldosterone-rennin detection while a novel mutation is discovered.

Blood Press 2022 12;31(1):139-145

Department of Endocrinology, Shanghai Public Health Clinical Center, Shanghai, China.

Purpose: Through describing the confusing misdiagnosis process of Liddle syndrome, we try to reveal the importance of accurate aldosterone-renin detection and a genetic test for Liddle syndrome.

Methods: We found a family of hypertension and hypokalaemia with the proband of a 21-year-old female who had been misdiagnosed as primary aldosteronism (PA). She presented with high aldosterone and low renin levels. Aldosterone is not suppressed in the saline infusion test and captopril challenge test. However, treatment with a standard dose of spironolactone has no blood pressure improvement effect. A heterozygous variant of was found with whole exome sequencing and Liddle syndrome is indicated. Treatment with amiloride was effective. We rechecked aldosterone-renin levels with two different aldosterone and renin test kits. Clinical features and the mutant gene SCNN1G of each family member were determined by the Sanger method.

Results: The two kits had nearly opposite results. Among those Liddle syndrome patients confirmed by a genetic test, for Test kit A all ARR were screened positive while for test kit B negative. It seems Test kit B is consistent with the diagnosis while test kit A misleads the diagnosis. A novel mutation, c.1729 C > T, was found in this family, which introduce a premature stop codon in the γ subunit in the epithelial Na channel (ENaC) and resulted in a deletion of 72 amino acids at the carboxyl end.

Conclusion: inaccurate ARR detection might misdiagnose Liddle syndrome. A Gene test is an important method for the diagnosis of Liddle syndrome. A novel missense mutation, c.1729 C > T, is found in a Chinese family.
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http://dx.doi.org/10.1080/08037051.2022.2088471DOI Listing
December 2022

Benzo[de]isoquinoline-1,3-dione condensed asymmetric azaacenes as strong acceptors.

RSC Adv 2022 Apr 4;12(21):13480-13486. Epub 2022 May 4.

College of Chemistry and Chemical Engineering, Northwest Normal University Lanzhou 730070 China

We have designed and synthesized three novel benzo[de]isoquinoline-1,3-dione (BQD) condensed asymmetric azaacenes, BQD-TZ, BQD-AP and BQD-PA, with different end groups of 1,2,5-thiadiazole, acenaphthylene and phenanthrene. The triisopropylsilylethynyl groups were grafted to increase the solubility and crystallinity of the three molecules. These molecules have high electron affinity values of 3.87, 3.69 and 3.74 eV for BQD-TZ, BQD-AP and BQD-PA, respectively as confirmed by cyclic voltammetry measurements. Single-crystal X-ray diffraction revealed that these molecules have strong π-π stacking with distances of 3.31-3.41 Å and different stacking arrangements.
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http://dx.doi.org/10.1039/d2ra01074gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067369PMC
April 2022

Antibody-drug conjugates targeting CD248 inhibits liver fibrosis through specific killing on myofibroblasts.

Mol Med 2022 03 22;28(1):37. Epub 2022 Mar 22.

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, 710072, China.

Background: Chronic liver injury induces pathological repair, resulting in fibrosis, during which hepatic stellate cells (HSCs) are activated and transform into myofibroblasts. CD248 is mainly expressed on myofibroblasts and was considered as a promising target to treat fibrosis. The primary aim of this study was to generate a CD248 specific antibody-drug conjugate (ADC) and evaluate its therapeutic efficacy for liver fibrosis and its safety in vivo.

Methods: CD248 expression was examined in patients with liver cirrhosis and in mice with CCl-induced liver fibrosis. The ADC IgG78-DM1, which targets CD248, was prepared and its bioactivity on activated primary HSCs was studied. The anti-fibrotic effects of IgG78-DM1 on liver fibrosis were evaluated in CCl-induced mice. The reproductive safety and biosafety of IgG78-DM1 were also evaluated in vivo.

Results: CD248 expression was upregulated in patients with liver cirrhosis and in CCl-induced mice, and was mainly expressed on alpha smooth muscle actin (α-SMA) myofibroblasts. IgG78-DM1 was successfully generated, which could effectively bind with and kill CD248 activated HSCs in vitro and inhibit liver fibrosis in vivo. In addition, IgG78-DM1 was demonstrated to have qualified biosafety and reproductive safety in vivo.

Conclusions: Our study demonstrated that CD248 could be an ideal target for myofibroblasts in liver fibrosis, and CD248-targeting IgG78-DM1 had excellent anti-fibrotic effects in mice with liver fibrosis. Our study provided a novel strategy to treat liver fibrosis and expanded the application of ADCs beyond tumors.
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http://dx.doi.org/10.1186/s10020-022-00460-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8939076PMC
March 2022

Detection Methods of Nanoparticles Synthesized by Gas-Phase Method: A Review.

Front Chem 2022 28;10:845363. Epub 2022 Feb 28.

Laboratory of Optical Detection and Imaging, School of Science, Qingdao University of Technology, Qingdao, China.

The detection of nanoparticles is the basis of the study of synthesis mechanism, active regulation of the synthesis process, and the study of nanoparticle properties after synthesis. It is significantly meaningful to the academia and engineering industry. Although there are many relevant detection methods at present, each method has its own advantages and disadvantages, and their measurement quantity and application conditions are also different. There is a lack of unified sorting and generalization. In this paper, the significance of detection of nanoparticles synthesized by a gas-phase method is introduced, the development of detection technology is reviewed, and the future is prospected. It is hoped that this paper will provide a reference for the detection of nanoparticles under various conditions and for the development of new detection methods.
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http://dx.doi.org/10.3389/fchem.2022.845363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919326PMC
February 2022

Ni-Fe Layered Double Hydroxide Nanosheets Supported on Exfoliated Graphite for Efficient Urea Oxidation in Direct Urea Fuel Cells.

ChemSusChem 2022 Apr 3;15(7):e202102614. Epub 2022 Mar 3.

Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, P. R. China.

Urea-rich wastewater can cause serious eutrophication problem to the water environment. On the other hand, urea is a potential fuel with high energy density, which can be effectively utilized by direct urea fuel cell. In this work, exfoliated graphite (EG) with high surface area and electrical conductivity was obtained by microwave irradiation, which was used to support the Ni-Fe layered double hydroxide (LDH), leading to a highly efficient and low-cost urea oxidation catalyst. Compared with commercial RuO , the as-prepared Ni-Fe LDH/EG exhibited a lower onset potential of 1.25 V vs. reversible hydrogen electrode as well as a lower Tafel slope of 44 mV dec . The catalyst durability was also proved to be excellent. The optimized Ni/Fe molar ratio was confirmed to be 3 : 1, while the most suitable catalyst/EG ratio was 3 : 50. When applied in a dual-electrolyte direct urea fuel cell, the peak power density reached 12 mW cm , and the long-term discharge was also stable with negligible voltage loss at 10 mA cm for 3 h. Such a low-cost and efficient urea oxidation catalyst can be widely utilized in future direct urea fuel cells, which achieve wastewater treatment and renewable electricity generation at the same time.
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http://dx.doi.org/10.1002/cssc.202102614DOI Listing
April 2022

Cancellation method to improve signal to noise ratio of an electrodeless microwave-biased ZnO single crystal x-ray detector.

Rev Sci Instrum 2022 Jan;93(1):015006

The Key Lab of Micro-Nano Electronics and System Integration of Xi'an City, Faculty of Electronic and Information Engineering, School of Microelectronics, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.

Semiconductor x-ray detectors are usually fabricated with proper ohmic or Schottky contact electrodes, which make the fabrication process complex and even unable to realize, especially for new materials. In this paper, we demonstrated an electrodeless ZnO single crystal x-ray detector using microwave (MW) bias with a high signal-to-noise ratio obtained by a cancellation method. The MW-biased x-ray detector is fabricated using the split-ring-resonator with the ZnO crystal mounted on the split-ring gap. The analytical response model was built for the detector. The MW cancellation process was realized by a phase and amplitude matching network. By using the cancellation method, the signal-to-noise ratio of the detector is about 59.4 dB, which is 58 dB higher than that of the DC-biased ZnO photodetector. The sensitivity of the detector is 139 µC Gy cm for the x-ray dose rate of 3.54 Gy/s, which is 86 times higher than that of the DC-biased ZnO photodetector. The high sensitivity of the detector is due to the high equivalent stimulated voltage caused by the split-ring resonator. The MW-biased detector can be used for x-ray dose monitoring.
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http://dx.doi.org/10.1063/5.0070988DOI Listing
January 2022

A patient with glucocorticoid hypersensitivity syndrome.

Chin Med J (Engl) 2022 Jan 27. Epub 2022 Jan 27.

Department of Endocrinology and Metabolism, Shanghai Public Health Clinical Center, Shanghai Medical School, Fudan University, Shanghai, China Department of Endocrinology and Metabolism, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai, China.

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http://dx.doi.org/10.1097/CM9.0000000000001977DOI Listing
January 2022

CD248 as a bridge between angiogenesis and immunosuppression: a promising prognostic and therapeutic target for renal cell carcinoma.

Ann Transl Med 2021 Dec;9(23):1741

Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Background: Renal cell carcinoma (RCC) is characterized by significant vascularization and immunogenicity, which contributes to drug resistance and immune escape. CD248, a pericytes marker in tumor vasculature, might help explain tumor microenvironment (TME) remodeling and serve as a novel therapeutic target.

Methods: Transcriptome data and clinical information of RCC patients were obtained from The Cancer Genome Atlas (TCGA) database. ESTIMATE and microenvironment cell population (MCP)-counter algorithms were adopted to calculate immune and stromal contents. The prognostic value of TME was evaluated via Kaplan-Meier and Wilcoxon signed rank test. Pearson's correlation coefficient was employed to explore the correlation between angiogenesis and TME, and the relationship between CD248 and TME or RCC progression. CD248 overexpression and vascular colocalization in RCC were confirmed via histology staining. The weighted gene coexpression network analysis (WGCNA) and enrichment analysis were performed to explore CD248-mediated regulatory mechanism in angiogenesis and TME remodeling. CD248-based drug response was predicted through CellMiner database.

Results: Tumor angiogenesis contributed to deteriorated RCC progression, which might be involved with immunosuppression. More specifically, upregulated immune checkpoints exhausted infiltrated T cells. CD248 overexpressed in RCC vessels correlated with TME and predicted a bad survival outcome. CD248 and coexpressed genes participated in angiogenesis and TME remodeling. Several clinical approved drugs that might inhibit CD248-mediated tumor promoting effects were selected.

Conclusions: CD248 appears to contribute to angiogenesis and immunosuppressive TME, and may thus be a promising prognostic and therapeutic target for RCC. CD248-based medication guidance might benefit RCC patients.
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http://dx.doi.org/10.21037/atm-21-6271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743697PMC
December 2021

Manganese-Doped Carbon Dots with Redshifted Orange Emission for Enhanced Fluorescence and Magnetic Resonance Imaging.

ACS Appl Bio Mater 2021 02 3;4(2):1969-1975. Epub 2021 Feb 3.

College of Chemistry and Environmental Science, Institute of Life Science and Green Development, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, P. R. China.

Metal-doped carbon dots (CDs) exhibited promising application in fluorescence and magnetic resonance (MR) imaging, but developing manganese-doped CDs (Mn-CDs) with long wavelength emission and enhanced MR performance is a challenge. Herein, using a one-step solvothermal method, Mn-CDs with redshifted orange emission and enhanced longitudinal relaxation were synthesized for fluorescence and MR imaging. The results indicated that the prepared Mn-CDs had a uniform size distribution, and the average size was 5 nm. Moreover, Mn-CDs possessed a stronger fluorescence performance than Mn-free CDs, and simultaneously, the emission wavelength can redshift from 542 nm (green emission) to 578 nm (orange emission), owing to the increasing N-doping. Because of the movement limit of Mn, Mn-CDs exhibited high T-weighted MR imaging performance with a longitudinal relaxation rate of 12.69 mM·s. The experiments demonstrated their excellent fluorescence and MR imaging with safety and reliability. Therefore, the prepared Mn-CDs with orange emission can be an excellent candidate as a dual-modal nanoprobe for fluorescence and MR imaging.
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http://dx.doi.org/10.1021/acsabm.0c01597DOI Listing
February 2021

Antibody-drug conjugates targeting CD248 myofibroblasts effectively alleviate renal fibrosis in mice.

FASEB J 2022 02;36(2):e22102

Institute of Medical Research, Northwestern Polytechnical University, Xi'an, China.

Myofibroblasts, or activated fibroblasts, play a critical role in the process of renal fibrosis. Targeting myofibroblasts to inhibit their activation or induce specific cell death has been considered to be an effective strategy to attenuate renal fibrosis. However, specific biomarkers for myofibroblasts are needed to ensure the efficacy of these strategies. Here, we verified that CD248 was mainly expressed in myofibroblasts in patients with chronic kidney disease, which was inversely correlated with renal function. The same result was also confirmed in renal fibrotic mice induced by unilateral ureteral obstruction and aristolochic acid nephropathy. By using an antibody-drug conjugate (ADC) named IgG78-DM1, in which maytansinoid (DM1) was linked to a fully human antibody IgG78 through an uncleavable SMCC linker, we demonstrated that it could effectively bind with and kill CD248 fibroblasts in vitro and alleviate renal fibrosis in mice models. Besides, we confirmed that IgG78-DM1 had qualified biosafety in vivo. Our results confirmed that CD248 can be used as a specific marker for myofibroblasts, and specific killing of CD248 myofibroblasts by IgG78-DM1 has excellent anti-fibrotic effect in renal fibrotic mice. Our study expanded the application of ADC and provided a novel strategy for the treatment of renal fibrosis.
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http://dx.doi.org/10.1096/fj.202101441RDOI Listing
February 2022

CD248 Cancer-Associated Fibroblasts: A Novel Prognostic and Therapeutic Target for Renal Cell Carcinoma.

Front Oncol 2021 14;11:773063. Epub 2021 Dec 14.

Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Background: The tumor microenvironment (TME) plays an important role in the progression of renal cell carcinoma (RCC). Cancer-associated fibroblasts (CAFs) are considered to constitute a major component of the TME and participate in various tumor-promoting molecular events. We have previously confirmed that CD248 represents a promising biomarker of CAFs, which may provide insight into CAF-based tumor-promoting effects. However, CAF-mediated tumor progression and the potential mechanism of CD248 remain largely unknown in RCC patients.

Methods: Expression profiling and clinical data of RCC patients were obtained from The Cancer Genome Atlas (TCGA) database. An MCP-counter algorithm and Kaplan-Meier survival analysis were performed to explore the prognostic value of CAFs and CD248, respectively. A Pearson correlation coefficient test and Student's -test were employed to evaluate the relationship between immunosuppressive TME and CD248 or CAFs. Immunohistochemistry and immunofluorescence staining were performed to confirm CD248 expression within CAFs. CD248-specific siRNA was used to investigate the potential function of CD248 in CAF tumor promotion. Differentially expressed genes (DEGs), weighted gene co-expression network analysis (WGCNA), and enrichment analysis were conducted to clarify the function of CD248 CAFs in RCC progression and the associated regulatory mechanism.

Results: CD248 overexpression and CAF infiltration could predict poor RCC prognosis, which may involve the immunosuppressive TME. CD248 may serve as a promising CAFs biomarker and be involved with the tumor-promoting effect of CAFs. Moreover, CD248 CAF infiltration may contribute to RCC progression and an immunosuppressive TME through cell-extracellular matrix (ECM) interactions and metabolism regulation.

Conclusion: CD248 CAFs participate in the regulation of RCC progression and immunosuppressive TME, which may represent a novel prognostic and therapeutic target for RCC.
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http://dx.doi.org/10.3389/fonc.2021.773063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712640PMC
December 2021

High-Performance MnO /Al Battery with In Situ Electrochemically Reformed Al MnO Nanosphere Cathode.

Small Methods 2021 Sep 1;5(9):e2100491. Epub 2021 Aug 1.

Department of Mechanical Engineering, The University of Hong Kong, Hong Kong, China.

Aqueous Al-ion battery (AAIB) is regarded as a promising candidate for large-scale energy storage systems due to its high capacity, high safety, and low cost, with MnO proved to be a high-performance cathode. However, the potential commercial application of this type of battery is plagued by the frequent structural collapse of MnO . Herein, an in situ, electrochemically reformed, urchin-like Al MnO cathode is developed for water-in-salt electrolyte-based AAIBs. Benefiting from its unique α-MnO coated Mn AlO structure, a high Al ion storage capacity is achieved together with a high discharge voltage plateau of 1.9 V by reversible MnO electrolysis. Consequently, the battery exhibits a high specific capacity of 285 mAh g and a high energy density of 370 Wh kg at a high current density of 500 mA g . Improved stability with record capacity retention is also obtained at an ultrahigh current density of 5 A g after 500 cycles. Such a high-capacity and high-stability Al MnO cathode would pave the way for in situ electrochemical transformation of cathode design and thus boost the practical application of AAIBs.
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http://dx.doi.org/10.1002/smtd.202100491DOI Listing
September 2021

The Beta-Cell Function and Glucose Profile of Newly Diagnosed Acromegalic Patients with Normal Glucose Tolerance.

Int J Endocrinol 2021 7;2021:3666692. Epub 2021 Dec 7.

Department of Endocrinology, Huashan Hospital Fudan University, Shanghai, China.

Objectives: Untreated acromegaly is a nature model for unveiling the diabetogenic effects of GH. CGMS can uncover more glucose profile of acromegaly. This study aimed to evaluate the insulin resistance (IR), -cell function, and glycemic spectrum of patients with newly diagnosed acromegaly with normal glucose tolerance (NGT).

Methods: This study was conducted in Huashan Hospital from January 2015 to February 2019. Eight newly diagnosed acromegalic patients without history of diabetes and eight age- and gender-matched healthy subjects were enrolled. All participants underwent oral glucose tolerance test (OGTT) and 72 h continuous glucose monitoring (CGM). Parameters on -cell function and IR were calculated. Mean blood glucose (MBG) in 24 hours was adopted for the evaluation of the glycemic level, and standard deviation of blood glucose (SDBG) and mean amplitude of glycemic excursion (MAGE) were used for glucose fluctuation.

Results: HbA1c in the acromegaly group was significantly higher than in the control. During OGTT, glucose peaked at 60 min in acromegaly and at 30 min in controls. After glucose load, the acromegaly group had significantly higher insulin levels than controls, especially in 120 min and 180 min. Both insulin sensitivity index and disposal index after glucose load of acromegaly were significantly lower than those of controls. Moreover, acromegalic subjects had significantly higher MBG than controls.

Conclusions: The newly diagnosed acromegalic patients with NGT were characterized by IR and impaired -cell function after glucose load. CGM showed that MBG of NGT acromegaly patients was higher than that of normal people.
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http://dx.doi.org/10.1155/2021/3666692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670947PMC
December 2021

Evolution of the conductive filament system in HfO-based memristors observed by direct atomic-scale imaging.

Nat Commun 2021 Dec 13;12(1):7232. Epub 2021 Dec 13.

Key Laboratory of Microelectronic Devices & Integration Technology, Institute of Microelectronics of Chinese Academy of Sciences, Beijing, 100029, China.

The resistive switching effect in memristors typically stems from the formation and rupture of localized conductive filament paths, and HfO has been accepted as one of the most promising resistive switching materials. However, the dynamic changes in the resistive switching process, including the composition and structure of conductive filaments, and especially the evolution of conductive filament surroundings, remain controversial in HfO-based memristors. Here, the conductive filament system in the amorphous HfO-based memristors with various top electrodes is revealed to be with a quasi-core-shell structure consisting of metallic hexagonal-HfO and its crystalline surroundings (monoclinic or tetragonal HfO). The phase of the HfO shell varies with the oxygen reservation capability of the top electrode. According to extensive high-resolution transmission electron microscopy observations and ab initio calculations, the phase transition of the conductive filament shell between monoclinic and tetragonal HfO is proposed to depend on the comprehensive effects of Joule heat from the conductive filament current and the concentration of oxygen vacancies. The quasi-core-shell conductive filament system with an intrinsic barrier, which prohibits conductive filament oxidation, ensures the extreme scalability of resistive switching memristors. This study renovates the understanding of the conductive filament evolution in HfO-based memristors and provides potential inspirations to improve oxide memristors for nonvolatile storage-class memory applications.
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http://dx.doi.org/10.1038/s41467-021-27575-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668918PMC
December 2021

Prognostic Value of Vascular-Expressed PSMA and CD248 in Urothelial Carcinoma of the Bladder.

Front Oncol 2021 17;11:771036. Epub 2021 Nov 17.

Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Background: Urothelial carcinoma of the bladder (UCB) is a common cancer of the urinary system. Despite substantial improvements in available treatment options, the survival outcome of patients with advanced UCB is unsatisfactory. Therefore, it is necessary to identify new prognostic biomarkers for monitoring and therapy guidance of UCB. In recent years, prostate-specific membrane antigen (PSMA) and CD248 have been identified promising candidate bio7markers.

Methods: In this study, we first examined PSMA and CD248 expression in tissues from 124 patients with UCB using immunohistochemical and immunofluorescent staining. We then analyzed the association between the expression of the two biomarkers and other clinicopathological features and prognosis. Finally, we performed bioinformatic analysis of and using the TCGA-BLCA dataset to explore the underlying mechanism of PSMA and CD248 in the progression of UCB.

Results: Among the 124 cases, PSMA and CD248 were confirmed to be expressed in tumor-associated vessels. Vascular PSMA and CD248 expression levels were associated significantly with several deteriorated clinicopathological features. Furthermore, using univariate and multivariate Cox analyses, high vascular PSMA and CD248 expression levels were observed to be associated significantly with poor prognosis in patients with UCB. As risk factors, both PSMA and CD248 expression showed good performance to predict prognosis. Furthermore, combining these vascular molecules with other clinical risk factors generated a risk score that could promote predictive performance. Bioinformatic analysis showed that both PSMA and CD248 might contribute to angiogenesis and promote further progression of UCB.

Conclusion: Both PSMA and CD248 are specifically expressed in the tumor-associated vasculature of UCB. These two molecules might be used as novel prognostic biomarkers and vascular therapeutic targets for UCB.
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http://dx.doi.org/10.3389/fonc.2021.771036DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635966PMC
November 2021

Active Suspension Control Strategy of Multi-Axle Emergency Rescue Vehicle Based on Inertial Measurement Unit.

Sensors (Basel) 2021 Oct 16;21(20). Epub 2021 Oct 16.

Key Laboratory of Special Carrier Equipment of Hebei Province, Yanshan University, Qinhuangdao 066004, China.

Active suspension control strategies are a top priority in active suspension system. The current research on active suspension control strategies is mostly focused on two-axle vehicles, and there is less research investigating multi-axle vehicles. Additionally, their effective implementation is dependent on accurate mathematical models, and most of them adopt force feedback control, which is vulnerable to external interference. To solve these problems, this paper proposes an active suspension control strategy based on Inertial Measurement Unit. The multi-axle emergency rescue vehicle is made to be equivalent to a 3-degrees-of-freedom parallel mechanism by using the method of grouping and interconnecting the suspension units of the whole vehicle. The attitude change of the vehicle body was transformed into the servo actuator's displacement by solving the inverse solution of the parallel mechanism position and the action of the servo actuator was driven in reverse according to the displacement obtained. In this way, the vehicle body attitude can be compensated, and the ride comfort and the handling stability of the vehicle can be improved. To verify the effectiveness of the control strategy proposed, the three-axle six vehicle was taken as the research object, the position inverse solution of its equivalent 3-degrees-of-freedom parallel mechanism was deduced, and a high-pass filter was designed. The three-axle vehicle experiment platform integrating active suspension and hydro-pneumatic suspension was built, and the gravel road and slope road experiments were carried out and the results compared with those obtained with hydro-pneumatic suspension. The experiment results showed that, compared with hydro-pneumatic suspension, the active suspension control strategy based on Inertial Measurement Unit proposed in this paper can not only stabilize the body attitude, but also effectively suppress body vibration, improving the ride comfort and handling stability of the vehicle significantly.
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http://dx.doi.org/10.3390/s21206877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8539746PMC
October 2021

Altered Lipid Profile Is a Risk Factor for the Poor Progression of COVID-19: From Two Retrospective Cohorts.

Front Cell Infect Microbiol 2021 30;11:712530. Epub 2021 Sep 30.

Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: The coronavirus disease 2019 (COVID-19) pandemic has spread worldwide. However, the impact of baseline lipid profile on clinical endpoints in COVID-19 and the potential effect of COVID-19 on lipid profile remain unclear.

Methods: In this retrospective cohort study, we consecutively enrolled 430 adult COVID-19 patients from two Chinese hospitals (one each in Chengdu and Wuhan). The lipid profile before admission and during the disease course and the clinical endpoint including in-hospital death or oropharyngeal swab test positive again (OSTPA) after discharge were collected. We used Kaplan-Meier and Cox regression to explore the lipid risk factors before admission associated with endpoints. Then, we assessed the lipid level change along with the disease course to determine the relationship between pathology alteration and the lipid change.

Results: In the Chengdu cohort, multivariable Cox regression showed that low-density lipoprotein cholesterol (LDL-C) dyslipidemia before admission was associated with OSTPA after discharge for COVID-19 patients (RR: 2.51, 95% CI: 1.19, 5.29, p = 0.006). In the Wuhan cohort, the patients with triglyceride (TG) dyslipidemia had an increased risk of in-hospital death (RR: 1.92, 95% CI: 1.08, 3.60, p = 0.016). In addition, in both cohorts, the lipid levels gradually decreased in the in-hospital death or OSTPA subgroups since admission. On admission, we also noticed the relationship between the biomarkers of inflammation and the organ function measures and this lipid level in both cohorts. For example, after adjusting for age, sex, comorbidities, smoking, and drinking status, the C-reactive protein level was negatively associated with the TC lipid level [β (SE) = -0.646 (0.219), = 0.005]. However, an increased level of alanine aminotransferase, which indicates impaired hepatic function, was positively associated with total cholesterol (TC) lipid levels in the Chengdu cohort [β (SE) = 0.633 (0.229), = 0.007].

Conclusions: The baseline dyslipidemia should be considered as a risk factor for poor prognosis of COVID-19. However, lipid levels may be altered during the COVID-19 course, since lipidology may be distinctly affected by both inflammation and organic damage for SARS-CoV-2.
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http://dx.doi.org/10.3389/fcimb.2021.712530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515140PMC
October 2021

High-Performance Harsh-Environment-Resistant GaO Solar-Blind Photodetectors via Defect and Doping Engineering.

Adv Mater 2022 Jan 19;34(1):e2106923. Epub 2021 Oct 19.

School of Microelectronics, University of Science and Technology of China, Hefei, 230026, China.

Gallium oxide (Ga O ), with an ultrawide bandgap, is currently regarded as one of the most promising materials for solar-blind photodetectors (SBPDs), which are greatly demanded in harsh environment, such as space exploration and flame prewarning. However, realization of high-performance SBPDs with high tolerance toward harsh environments based on low-cost Ga O material faces great challenges. Here, defect and doping (DD) engineering towards amorphous GaO (a-GaO ) has been proposed to obtain ultrasensitive SBPDs for harsh condition application. Serious oxygen deficiency and doping compensation of the engineered a-GaO film ensure the high response currents and low dark currents, respectively. Annealing item in nitrogen of DD engineering also incurs the recrystallization of material, formation of nanopores by oxygen escape, and suppression of sub-bandgap defect states. As a result, the tailored GaO SBPD based on DD engineering not only harvests a record-high responsivity rejection ratio (R /R ) of 1.8 × 10 , 10 times higher detectivity, and 2 × 10 times faster decay speed than the control device, but also keeps a high responsivity, high photo-to-dark current ratio, and sharp imaging capability even at high temperature (280 °C) or high bias (100 V). The proposed DD engineering provides an effective strategy towards highly harsh-environment-resistant GaO SBPDs.
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http://dx.doi.org/10.1002/adma.202106923DOI Listing
January 2022

The Impact of Glycolipid Metabolic Disorders on Severity Stage-Specific Variation of Cardiac Autonomic Function in Obstructive Sleep Apnea: A Data-Driven Clinical Study.

Nat Sci Sleep 2021 28;13:1347-1362. Epub 2021 Jul 28.

Department of Otolaryngology Head and Neck Surgery & Center of Sleep Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, People's Republic of China.

Background: Cardiac autonomic dysfunction (CAD) is a common pathology in cardiovascular diseases; however, the role of glycolipid metabolic disorders in CAD development in obstructive sleep apnea (OSA) remains poorly understood.

Methods: In total, 4152 patients with suspected OSA were recruited in our sleep center. Metabolic characteristics including anthropometric and glycolipid data were collected. Heart rate variability (HRV) was measured to assess the risk of CAD; its dose-response relationship with OSA severity was evaluated via restricted cubic spline (RCS) analysis. A segmented multivariate linear regression (SMLR) model was used to evaluate the roles of metabolic variables in different stages of OSA.

Results: The RCS showed that CAD risk increased in a nonlinear relationship pattern with OSA severity, from slow fluctuation at earlier stages to rapid change in later stages. After integrating the clinical definition and RCS selected knots, we obtained the new four OSA severity stages. SMLR model showed that the overall value of glycolipid variables for prediction of HRV abnormalities was greater than the value of OSA variables at earlier stages, while OSA variables were more effective predictors in more severe stages. The discordance in respective relationship of HRV with metabolic and OSA variables sheds the light how metabolic disorders promoted the development of CAD in OSA, the later further in turn deteriorates cardiac function.

Conclusion: These results are indicative of stage-specific involvement of glycolipid metabolic factors underlying CAD nonlinear changes in patients with OSA. Early control glycolipid disorders may help the control of CAD development in patients with OSA.
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http://dx.doi.org/10.2147/NSS.S317201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327800PMC
July 2021

Fully Printed High-Performance n-Type Metal Oxide Thin-Film Transistors Utilizing Coffee-Ring Effect.

Nanomicro Lett 2021 Aug 3;13(1):164. Epub 2021 Aug 3.

Key Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, 310024, China.

Metal oxide thin-films transistors (TFTs) produced from solution-based printing techniques can lead to large-area electronics with low cost. However, the performance of current printed devices is inferior to those from vacuum-based methods due to poor film uniformity induced by the "coffee-ring" effect. Here, we report a novel approach to print high-performance indium tin oxide (ITO)-based TFTs and logic inverters by taking advantage of such notorious effect. ITO has high electrical conductivity and is generally used as an electrode material. However, by reducing the film thickness down to nanometers scale, the carrier concentration of ITO can be effectively reduced to enable new applications as active channels in transistors. The ultrathin (~10-nm-thick) ITO film in the center of the coffee-ring worked as semiconducting channels, while the thick ITO ridges (>18-nm-thick) served as the contact electrodes. The fully inkjet-printed ITO TFTs exhibited a high saturation mobility of 34.9 cm V s and a low subthreshold swing of 105 mV dec. In addition, the devices exhibited excellent electrical stability under positive bias illumination stress (PBIS, ΔV = 0.31 V) and negative bias illuminaiton stress (NBIS, ΔV = -0.29 V) after 10,000 s voltage bias tests. More remarkably, fully printed n-type metal-oxide-semiconductor (NMOS) inverter based on ITO TFTs exhibited an extremely high gain of 181 at a low-supply voltage of 3 V, promising for advanced electronics applications.
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http://dx.doi.org/10.1007/s40820-021-00694-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333237PMC
August 2021

The Potential Use of Dynamics Changes of ctDNA and cfDNA in the Perioperative Period to Predict the Recurrence Risk in Early NSCLC.

Front Oncol 2021 16;11:671963. Epub 2021 Jul 16.

The Department of Thoracic Surgery, Daping Hospital, Army Medical University, Chongqing, China.

Background: Postoperative circulation tumor DNA (ctDNA) is a promising method to predict the risk of recurrence. However, the amount of ctDNA in patients with early NSCLC is too small. Cell damages caused during the intraoperative period leads to a significant increase in cell free DNA (cfDNA). Whether cfDNA content is restored to the preoperative level within a short time after surgery may indicate the degree of surgical trauma. In this study, dynamic changes of cfDNA combined with ctDNA in the perioperative period of NSCLC were used to explore the possibility of them as a biomarker to indicate the risk of recurrence.

Methods: NSCLC patients who planned to undergo radical resection were investigated. 10ml of peripheral blood was collected before, during and 7 days after surgery. DNA concentration was measured, and a 23-gene NGS panel was performed to detect gene mutations. All the patients would be followed-up for at least 18 months.

Results: A total of 7 patients were sampled. The amount of cfDNA before surgery was 36.6 ± 14.7ng, and increased to 127.2 ± 52.2ng during surgery. 7 days after surgery, it dropped to 45.23 ± 9.41ng in 3 patients and rose to 173.7 ± 80.80ng in the remaining 4. Only 1 patient was ctDNA positive after surgery, with decreasing cfDNA, and he was the only one that relapsed and died within 18 months.

Conclusion: The use of ctDNA to predict the risk of postoperative recurrence of NSCLC is a very valuable method, and it may be more reliable if combined with the dynamic changes of cfDNA. The amounts of cfDNA are raised by the operation, but will be polarized after surgery in 7 days. Postoperative NSCLC patients with positive ctDNA and reduced cfDNA have a higher risk of recurrence.
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http://dx.doi.org/10.3389/fonc.2021.671963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322676PMC
July 2021

A Study of Optimal Specifications for Light Shelves with Photovoltaic Modules to Improve Indoor Comfort and Save Building Energy.

Int J Environ Res Public Health 2021 03 4;18(5). Epub 2021 Mar 4.

School of Architecture, Kookmin University, 77, Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea.

Recent studies on light shelves found that building energy efficiency could be maximized by applying photovoltaic (PV) modules to light shelf reflectors. Although PV modules generate a substantial amount of heat and change the consumption of indoor heating and cooling energy, performance evaluations carried out thus far have not considered these factors. This study validated the effectiveness of PV module light shelves and determined optimal specifications while considering heating and cooling energy savings. A full-scale testbed was built to evaluate performance according to light shelf variables. The uniformity ratio was found to improve according to the light shelf angle value and decreased as the PV module installation area increased. It was determined that PV modules should be considered in the design of light shelves as their daylighting and concentration efficiency change according to their angles. PV modules installed on light shelves were also found to change the indoor cooling and heating environment; the degree of such change increased as the area of the PV module increased. Lastly, light shelf specifications for reducing building energy, including heating and cooling energy, were not found to apply to PV modules since PV modules on light shelf reflectors increase building energy consumption.
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http://dx.doi.org/10.3390/ijerph18052574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967301PMC
March 2021

The Diagnostic and Immunotherapeutic Value of CD248 in Renal Cell Carcinoma.

Front Oncol 2021 12;11:644612. Epub 2021 Mar 12.

Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Renal cell carcinoma (RCC) is the most common malignancy in the urinary system. Despite substantial improvements in available treatment options, the survival outcome of advanced RCC is unsatisfactory. Identifying novel biomarkers to assist in early diagnosis and to screen patients who are sensitive to immunotherapy would be beneficial. CD248 is a promising candidate that deserves to be investigated. The Cancer Genome Atlas (TCGA) data set and clinical specimens were adopted to analyze the expression of CD248 between normal and tumor tissues. Univariate and multivariate Cox regression analyses were employed to identify independent prognostic factors and construct a CD248-based prognostic signature. The correlation among the present signature, tumor-infiltrating immune cells (TIICs), the tumor mutation burden (TMB), and immunomodulatory molecules was evaluated. The weighted gene co-expression network analysis (WGCNA), the enrichment analysis, and the miRNA correlation analysis were performed to explore the underlying mechanism of CD248 in the progression of RCC. The overexpression of CD248 in RCC was related to a poor prognosis, and a CD248-based prognostic signature could precisely stratify patients with RCC with different survival outcomes regardless of the training or testing cohort. The present signature could reflect the immunosuppressive landscape of RCC (i.e., increased infiltration of regulatory T cells and upregulated immune checkpoints), accompanied by deteriorated clinicopathologic indexes. The TMB and immunostimulatory molecules expression also increased with the risk score generated from the present signature. CD248 co-expressed gene sets were identified through the WGCNA algorithm, and several immunosuppressive Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched. The result of CD248-correlated miRNA further emphasized the importance of CD248 in RCC. CD248 is a valuable biomarker to improve the diagnostic and therapeutic efficiency of RCC. The immunosuppressive effect of CD248 co-expressed genes may provide insight for the present study, and miRNA would help to reveal the mechanism of the expressive regulation of CD248.
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http://dx.doi.org/10.3389/fonc.2021.644612DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006336PMC
March 2021
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