Publications by authors named "Xiaoling Wu"

231 Publications

Insulin promotes hepatocarcinoma tumorigenesis by up-regulating PKM2 expression.

Exp Cell Res 2021 Oct 11:112872. Epub 2021 Oct 11.

Department of Gastroenterology, Zhongshan Hospital Affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian Province, P. R. China; Xiamen Key Laboratory of Intestinal Microbiome and Human Health, Zhongshan Hospital Affiliated to Xiamen University, 201 Hubin South Road, Xiamen 361004, Fujian Province, P. R. China; Faculty of Clinical Medicine, School of Medicine, Xiamen University, 168 University Road, Xiamen 361005, Fujian Province, P. R. China; Faculty of Clinical Medicine & Institute of Mirobial Ecology, Medical College of Xiamen University, 168 University Road, Xiamen 361005, Fujian Province, P. R. China; Department of Digestive Disease, School of Medicine, Xiamen University, 168 University Road, Xiamen 361005, Fujian Province, P. R. China. Electronic address:

Insulin, as a growth factor, can increase the risk of certain types of cancer. The present study showed that insulin promoted the proliferation of hepatocellular carcinoma cells in vitro and in vivo throughpyruvate kinase M2 (PKM2), which is a rate-limiting enzyme in the process of glycolysis. Moreover, the expression of PKM2 was up-regulated by insulin at the posttranslational levelin a nuclear orphan receptor TR3-dependent manner. In addition, insulin could enhance the interaction between PKM2 and TR3 and protect PKM2 from degradation. Our results identified a specific mechanism of insulin affecting cancer metabolism and thus promoting cancer progression, and they contribute to a better understanding of the observation that insulin is linked to an increased cancer risk under hyperinsulinemic conditions.
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http://dx.doi.org/10.1016/j.yexcr.2021.112872DOI Listing
October 2021

SP1-Regulated Non-Coding RNA SNHG22 Promotes Ovarian Cancer Growth and Glycolysis.

Cancer Manag Res 2021 21;13:7299-7309. Epub 2021 Sep 21.

Department of Gynecology, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Liaoning, 110042, People's Republic of China.

Objectives: Long non-coding RNAs (lncRNAs) play a crucial part in cancer progression. However, in epithelial ovarian carcinoma (EOC), the role of SNHG22 needs to be further explained.

Methods: Quantitative real-time PCR was used to detect the expression of SNHG22. EOC cells were stably transfected with lentivirus approach and cell proliferation, glycolysis and cell apoptosis, as well as tumorigenesis in animal were performed to assess the effects of SNHG22 in EOC. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay were conducted to confirm the relationship between SP1 and SNHG22.

Results: Higher expressed SNHG22 was associated with a poor prognosis in EOC tissues. SNHG22 facilitated glycolysis and proliferation. Mechanistically, LDHA deficiency and glycolysis inhibitor (2-DG, 3-BG) partly rescued proliferation. SP1 mediated SNHG22 expression at the transcriptional level and the SNHG22 promoter region (-900~ -600) was necessary for SP1 binding. Hypoxia and HIF-1α also upregulated SNHG22 expression.

Conclusion: SNHG22 is an independent prognostic biomarker for EOC. SNHG22 promotes EOC progression and is a prospective therapeutic target.
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http://dx.doi.org/10.2147/CMAR.S318378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464310PMC
September 2021

Efficacy of peripheral arterial access for peripheral blood stem cells collection.

J Clin Apher 2021 Sep 24. Epub 2021 Sep 24.

Department of Nursing, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, Shandong, China.

Background: Transplantation of peripheral blood stem cells (PBSCs) mobilized by cytokines is increasingly applied to treat patients with hematologic diseases, such as lymphoma, multiple myeloma, leukemia, etc. Successful hematopoietic stem cell transplantation (HSCT) increasingly depends on the collection of hematopoietic stem cells (HSCs) from peripheral blood. Peripheral vein (PV) is the most common type of blood access. When the blood vessels are not well filled and the blood flow is insufficient, the machine will appear repeated low pressure alarm or pipeline coagulation, which seriously affects the collection efficiency. A peripheral artery (PA) is utilized for drawing blood, while a peripheral vein is used for blood return, that is a way to perform apheresis. The advantages of PA are that it ensures adequate extracorporeal circulation blood flow, stable blood flow rate, simple operation, and relatively low price. However, there are very few studies on the efficacy of peripheral arterial access for HSCs collection. Therefore, this retrospective study was conducted to assess the effectiveness of PA and PV access for PBSCs collection.

Methods: We performed a retrospective analysis of 150 apheresis procedures on 26 patients and 95 healthy donors collected by PV or PA access from March 1, 2020 to March 1, 2021. We compared the CD34+ cell count, collection efficiency (CE), duration of processing a single blood volume, number of low-pressure alarms, average blood flow rate and number of punctures between the two groups. Also, we analyzed adverse events.

Results: There was no significant difference in the quality of apheresis blood components between the PA group and the PV group. The CD34+ cells collected was 274.16 ± 216.31 × 10 in the PV group and 246.63 ± 127.94 × 10 in the PA group. The CE in the PA group was 49.50 ± 9.88%, higher than 42.39 ± 14.62% in the PV group. The duration of processing a single blood volume was 90.67 ± 15.35 min in the PV group and 79.68 ± 10.28 min in the PA group. The number of low-pressure alarms in the PA group was 0.38 ± 0.98, <2.42 ± 1.76 in the PV group, and the average blood flow rate in the PA group was 59.27 ± 2.18, higher than 54.21 ± 3.41 in the PV group. The difference was statistically significant (P < .05). The Number of punctures was 1.35 ± 0.75 in the PA group and 1.41 ± 1.01 in the PV group. There was no statistically significant difference.

Conclusion: Peripheral artery is a safe, reliable, economical, convenient, and fast vascular access, which opens a new way to the establishment of vascular access for PBSCs collection.
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http://dx.doi.org/10.1002/jca.21940DOI Listing
September 2021

The Ferroptosis-NLRP1 Inflammasome: The Vicious Cycle of an Adverse Pregnancy.

Front Cell Dev Biol 2021 20;9:707959. Epub 2021 Aug 20.

Department of Traditional Chinese Medicine, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

One of the hallmarks of placental dysfunction is the increase of oxidative stress. This process, along with the overexpression of the inflammasome, creates a downward spiral that can lead to a series of severe pregnancy complications. Ferroptosis is a form of iron-mediated cell death involving the accumulation of reactive oxygen species, lipid peroxides. In this study, the rats' model of oxidative stress abortion was established, and hydrogen peroxide (HO) was used to establish a cellular model of placental oxidative stress. RNAi, western blot, and immunofluorescence were used to evaluate the expression of specific markers of ferroptosis and the expression of the inflammasome in placental trophoblast cells. We observed excessive levels of ferroptosis and inflammasome activation in both rats' model and placental trophoblast cell model of oxidative stress. When the NLRP1 inflammasome was silenced, the expression levels of GSH and Glutathione peroxidase 4 (GPX4) were increased, while the expression levels of transferrin receptor 1 (TFR1), acyl-CoA synthetase long-chain family member 4 (ACSL4), Superoxide dismutase (SOD), and Malondialdehyde (MDA) were decreased. However, when an NLRP1 activator was applied, we observed the opposite phenomenon. We further explored the mechanisms underlying the actions of ferroptosis to inflammasomes. The expression levels of NLRP1, NLRP3, IL-1β, and caspase-1 were positively correlated with the ferroptosis following the application of ferroptosis inhibitor (ferrostatin-1) and ferroptosis activator (erastin). The existence of ferroptosis was demonstrated in the oxidative stress model of placental trophoblast cells; the results also indicate ferroptosis is linked with the expression of NLRP1 inflammasome. These findings may provide a valuable therapeutic target for the pathogenesis of pregnancy-related diseases.
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http://dx.doi.org/10.3389/fcell.2021.707959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417576PMC
August 2021

Downregulation of GTSE1 leads to the inhibition of proliferation, migration, and Warburg effect in cervical cancer by blocking LHDA expression.

J Obstet Gynaecol Res 2021 Sep 5. Epub 2021 Sep 5.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.

Aim: G2 and S phase-expressed-1 (GTSE1) has been identified to play a vital role in several kinds of cancers, but its role in cervical cancer development remains unknown. Herein, we aimed to reveal the role and underlying mechanism of GTSE1 in cervical cancer cell growth, migration, and aerobic glycolysis.

Methods: GTSE1 expression levels in cervical cancer tissues and normal cervical tissues were determined by real time PCR and immunohistochemistry. Human short hairpin RNA was used to downregulate GTSE1 level in cervical cancer cells SiHa and HeLa cells. Colony formation, cell counting kit-8, and wound-healing assays were used for cell function evaluation. Lactate production, lactate dehydrogenase activity, and glucose concentration were tested to assess the Warburg effect.

Results: GTSE1 expressions at both mRNA and protein levels were significantly elevated in cervical cancer tissues compared with normal tissues. Downregulation of GTSE1 induced significant repressions in cell colony formation, viability and migration, and Warburg effect, as well as reduced expression of lactate dehydrogenase isoform A (LDHA) at mRNA and protein levels. Additionally, downregulation of GTSE1 weakened the tumorigenesis of HeLa and SiHa cells in vivo.

Conclusion: This study demonstrated that downregulation of GTSE1 led to significant inhibitions in cell proliferation, migration, tumorigenesis, and Warburg effect in cervical cancer by blocking the expression of LHDA.
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http://dx.doi.org/10.1111/jog.15000DOI Listing
September 2021

Effects of on Production Performance, Bone Physiological Property, and Hematology Indexes in Laying Hens.

Animals (Basel) 2021 Jul 8;11(7). Epub 2021 Jul 8.

Livestock Behavior Research Unit, USDA-Agricultural Research Service, West Lafayette, IN 47907, USA.

This study was to investigate the effects of on production performance and bone pathophysiological characteristics of layers. Twenty-four 48-week-old Lohmann Pink-shell laying hens were randomly divided into two groups: a basic diet (control) and the basic diet mixed with (0.5 g/kg) for a 60-day trial. Statistically, independent-sample -test was used to assess the treatment differences. The results showed that supplementation improved the percent of marketable eggs ( < 0.05) with reduced numbers of broken and soft-shelled eggs but had no effects on egg weight, height of albumen, yolk color, and Haugh unit ( > 0.05). supplement also elevated maximum load ( = 0.06), maximum stress ( = 0.01), stiffness ( < 0.01), and Young's modulus ( < 0.01) but suppressed maximum strain ( = 0.06) in the femur. In addition, compared with control birds, phosphorous concentration ( < 0.01) was reduced in serum at day 61 but increased in the femur ( < 0.05) in fed birds. fed birds also had lower magnesium concentrations in both femur ( = 0.04) and feces ( = 0.09). Furthermore, increased plasma estrogen concentration ( = 0.01) and femur TNF receptor superfamily member 11b () expression ( < 0.05) but reduced plasma IL-1 ( < 0.01) and TNF-α ( < 0.01) concentrations. These results indicate that could be used as a health promotor to reduce overproduction-induced inflammation and associated bone damage and to increase marketable egg production. The data provide evidence for developing a management strategy to use as a feed additive to improve marketable egg production and health and welfare status of laying hens.
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http://dx.doi.org/10.3390/ani11072041DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300237PMC
July 2021

microRNA-196b promotes esophageal squamous cell carcinogenesis and chemoradioresistance by inhibiting EPHA7, thereby restoring EPHA2 activity.

Am J Cancer Res 2021 15;11(7):3594-3610. Epub 2021 Jul 15.

Department of Medicine, Division of Genomic Medicine, Department of Microbiology, Immunology and Tropical Medicine, The George Washington University School of Medicine and Health Sciences Washington, DC, USA.

Esophageal cancer (EC) is extremely aggressive and has a very poor survival rate. Esophageal squamous cell carcinoma (ESCC) accounts for 80% of all ECs worldwide, with the majority of the remaining 20% being esophageal adenocarcinoma (EAC). Due to its occult and insidious presentation, ESCC is typically diagnosed and treated in its advanced stages, thereby limiting the success of present therapeutic modalities. microRNAs (miRNAs) can function as tumor suppressors or oncogenes, playing critical roles in cancer initiation and progression by regulating target genes in oncogenic pathways. In the current study, we demonstrated that microRNA-196b (miR-196b) is one of the most upregulated miRNAs in both ESCC and EAC. miR-196b was overexpressed in ESCC and EAC cell lines, cellular exosomal RNAs, and ESCC tissue samples. Functional studies revealed that miR-196b acted as an oncomiR by directly targeting a tumor suppressor, ephrin type-A receptor 7 (EPHA7). EPHA7 abrogates the activity of ephrin type-A receptor 2 (EPHA2), a key molecule involved in the epithelial-to-mesenchymal transition (EMT) and MAPK/ERK pathways, mediating resistance to UV and chemoradiotherapy in both ESCC and EAC. Taken together, these findings suggest that miR-196b is a strong candidate molecular target for EC treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8332861PMC
July 2021

MicroRNA-148a-3p inhibits the proliferation of cervical cancer cells by regulating the expression levels of DNMT1 and UTF1.

Oncol Lett 2021 Aug 24;22(2):617. Epub 2021 Jun 24.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

MicroRNAs (miRs) serve a key role in carcinogenesis. miR-148a-3p has been demonstrated to act as a tumor suppressor in several tumors, such as epithelial ovarian cancer and esophageal cancer. However, to the best of our knowledge, the role of miR-148a-3p in cervical cancer remains unclear. In the present study, the expression levels of miR-148a-3p measured by reverse transcription-quantitative PCR were significantly decreased in cervical cancer tissues compared with that in normal cervical tissues. Furthermore, overexpression of miR-148a-3p markedly suppressed the proliferation of cervical cancer cells. The luciferase reporter assay demonstrated that DNA methyltransferase 1 (DNMT1) was the target gene of miR-148a-3p and that its expression measured by western blotting was inhibited by miR-148a-3p in cervical cancer cells. Correlation analysis highlighted that the expression levels of the undifferentiated embryonic cell transcription factor-1 (UTF1) were negatively associated with the expression levels of DNMT1 in cervical cancer tissues. Furthermore, DNMT1 knockdown increased the expression of UTF1 and decreased the methylation level of UTF1 promoter. These data demonstrated the expression levels of UTF1 were regulated by DNMT1 methylation in cervical cancer cells. Collectively, the results of the present study suggested that miR-148a-3p may inhibit the proliferation of cervical cancer cells by regulating the expression levels of DNMT1/UTF1, which provides potential therapeutic targets for cervical cancer.
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http://dx.doi.org/10.3892/ol.2021.12878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243077PMC
August 2021

Identification of a seven-long non-coding RNA signature associated with Jab1/CSN5 in predicting hepatocellular carcinoma.

Cell Death Discov 2021 Jul 10;7(1):178. Epub 2021 Jul 10.

Department of Systems Biology, The University of Texas - MD Anderson Cancer Center, Houston, TX, USA.

Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide, accounting for over 700,000 deaths each year. The lack of predictive and prognostic biomarkers for HCC, with effective therapy, remains a significant challenge for HCC management. Long non-coding RNAs (lncRNAs) play a key role in tumorigenesis and have clinical value as potential biomarkers in the early diagnosis and prediction of HCC. Jun activation domain-binding protein 1 (Jab1, also known as COP9 signalosome subunit 5, CSN5) is a potential oncogene that plays a critical role in the occurrence of HCC. Here, we performed a comprehensive analysis for Jab1/CSN5-associated lncRNAs to predict the prognosis of HCC. The differentially expressed (DE) lncRNAs between in HCC were analyzed based on the TCGA RNA-seq data. We detected 1031 upregulated lncRNAs in 371 HCC tissues and identified a seven-lncRNA signature strongly correlated with Jab1/CSN5 (SNHG6, CTD3065J16.9, LINC01604, CTD3025N20.3, KB-1460A1.5, RP13-582O9.7, and RP11-29520.2). We further evaluated the prognostic significance of these lncRNAs by GEPIA ( http://gepia.cancer-pku.cn/ ). The expression data in 364 liver tumors indicated that this seven-lncRNA signature could better predict worse survival in HCC patients. Moreover, 35 clinical HCC samples were evaluated to assess the validity and reproducibility of the bioinformatic analysis. We found that the targeted lncRNAs were upregulated, with a strong association with Jab1/CSN5 and prognostic value in HCC. Functional enrichment analysis by Gene Ontology (GO) showed that these seven prognostic lncRNAs exhibit oncogenic properties and are associated with prominent hallmarks of cancer. Overall, our findings demonstrate the clinical implication of Jab1/CSN5 with the seven-lncRNAs in predicting survival for patients with HCC.
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http://dx.doi.org/10.1038/s41420-021-00560-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272716PMC
July 2021

The internal exposure of bisphenol analogues in South China adults and the associated health risks.

Sci Total Environ 2021 Nov 2;795:148796. Epub 2021 Jul 2.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou, China. Electronic address:

Bisphenol A (BPA) is widely applied in industrial products and household products, leading to ubiquitous occurrences in environmental and biological samples. However, knowledge on human internal exposure to bisphenol analogues remains limited. Our study determined nine bisphenol analogues in urine samples collected from 1168 South China adults. BPA and bisphenol F (BPF) exhibited the highest detection frequencies in urine, i.e., 99.4% and 74.6%, respectively. BPA dominated over other analogues, with a median concentration of 1.74 μg/L, while BPF had a median concentration of 0.08 μg/L. Significant positive correlation was observed between urinary BPA and BPF (r = 0.201, p < 0.01), indicating similar exposure sources or pathways of these two chemicals. Urinary BPA concentrations were significantly correlated with age, marital status, drinking status and history of hyperlipidemia (p < 0.05). The median estimated daily intake (EDI) of ΣBPs (the sum concentrations of BPA, BPF and BPAF) was determined to be 53.6 ng/kg-bw/day for adults. The EDIs were much lower than the temporary tolerable reference dose of BPA recommended by the European Food Safety Authority, indicating the bisphenol analogues presented no obvious health risks to South China adults.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148796DOI Listing
November 2021

Perchlorate in shellfish from South China Sea and implications for human exposure.

Mar Pollut Bull 2021 Sep 1;170:112672. Epub 2021 Jul 1.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou 510275, China. Electronic address:

Shellfish can absorb and accumulate contaminants. The consumption of shellfish could expose humans to pollutants and increase related health risk. Perchlorate (ClO) is a ubiquitous pollutant and could affect thyroid functions, especially for children and pregnant women. However, knowledge on the contamination of perchlorate in aquatic food such as shellfish remains limited. This study aimed to investigate the abundances of perchlorate in shellfish from South China Sea and to assess human exposure risks. A total of 178 shellfish samples from eight species were collected from offshore aquaculture waters in South China Sea. Perchlorate was detected in 99.4% of them, suggesting widespread pollution in coastal waters. Concentrations of perchlorate ranged from not detected (N.D.) to 71.5 μg kg, with a median value of 4.33 μg kg. Estimated daily intake (EDI) and hazard quotient (HQ) were used to assess human exposure dose and health risks, respectively. The HQ values were determined to be less than 1, indicating no significant health risks to local residents via shellfish consumption. To our knowledge, this is the first study to investigate perchlorate contamination in South China shellfish and assess potential human risks.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112672DOI Listing
September 2021

Ciltacabtagene autoleucel, a B-cell maturation antigen-directed chimeric antigen receptor T-cell therapy in patients with relapsed or refractory multiple myeloma (CARTITUDE-1): a phase 1b/2 open-label study.

Lancet 2021 07 24;398(10297):314-324. Epub 2021 Jun 24.

Legend Biotech USA, Piscataway, NJ, USA.

Background: CARTITUDE-1 aimed to assess the safety and clinical activity of ciltacabtagene autoleucel (cilta-cel), a chimeric antigen receptor T-cell therapy with two B-cell maturation antigen-targeting single-domain antibodies, in patients with relapsed or refractory multiple myeloma with poor prognosis.

Methods: This single-arm, open-label, phase 1b/2 study done at 16 centres in the USA enrolled patients aged 18 years or older with a diagnosis of multiple myeloma and an Eastern Cooperative Oncology Group performance status score of 0 or 1, who received 3 or more previous lines of therapy or were double-refractory to a proteasome inhibitor and an immunomodulatory drug, and had received a proteasome inhibitor, immunomodulatory drug, and anti-CD38 antibody. A single cilta-cel infusion (target dose 0·75 × 10 CAR-positive viable T cells per kg) was administered 5-7 days after start of lymphodepletion. The primary endpoints were safety and confirmation of the recommended phase 2 dose (phase 1b), and overall response rate (phase 2) in all patients who received treatment. Key secondary endpoints were duration of response and progression-free survival. This trial is registered with ClinicalTrials.gov, NCT03548207.

Findings: Between July 16, 2018, and Oct 7, 2019, 113 patients were enrolled. 97 patients (29 in phase 1b and 68 in phase 2) received a cilta-cel infusion at the recommended phase 2 dose of 0·75 × 10 CAR-positive viable T cells per kg. As of the Sept 1, 2020 clinical cutoff, median follow-up was 12·4 months (IQR 10·6-15·2). 97 patients with a median of six previous therapies received cilta-cel. Overall response rate was 97% (95% CI 91·2-99·4; 94 of 97 patients); 65 (67%) achieved stringent complete response; time to first response was 1 month (IQR 0·9-1·0). Responses deepened over time. Median duration of response was not reached (95% CI 15·9-not estimable), neither was progression-free survival (16·8-not estimable). The 12-month progression-free rate was 77% (95% CI 66·0-84·3) and overall survival rate was 89% (80·2-93·5). Haematological adverse events were common; grade 3-4 haematological adverse events were neutropenia (92 [95%] of 97 patients), anaemia (66 [68%]), leukopenia (59 [61%]), thrombocytopenia (58 [60%]), and lymphopenia (48 [50%]). Cytokine release syndrome occurred in 92 (95%) of 97 patients (4% were grade 3 or 4); with median time to onset of 7·0 days (IQR 5-8) and median duration of 4·0 days (IQR 3-6). Cytokine release syndrome resolved in all except one with grade 5 cytokine release syndrome and haemophagocytic lymphohistiocytosis. CAR T-cell neurotoxicity occurred in 20 (21%) patients (9% were grade 3 or 4). 14 deaths occurred in the study; six due to treatment-related adverse events, five due to progressive disease, and three due to treatment-unrelated adverse events.

Interpretation: A single cilta-cel infusion at the target dose of 0·75 × 10 CAR-positive viable T cells per kg led to early, deep, and durable responses in heavily pretreated patients with multiple myeloma with a manageable safety profile. The data from this study formed the basis for recent regulatory submissions.

Funding: Janssen Research & Development and Legend Biotech.
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http://dx.doi.org/10.1016/S0140-6736(21)00933-8DOI Listing
July 2021

Ultrasensitive immunochromatographic strip for the detection of cyhalothrin in foods.

Anal Methods 2021 07 16;13(27):3040-3049. Epub 2021 Jun 16.

State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

In this study, a highly specific and sensitive monoclonal antibody (mAb) against lambda-cyhalothrin (LCT) was prepared. The half maximal inhibitory concentration (IC) in the ic-ELISA was 1.2 ng mL and the limit of detection (LOD) value was 0.2 ng mL. Based on the mAb, an immunochromatographic strip (ICS) was developed to qualitatively and quantitatively detect LCT in cabbage, parsley, spinach, and green tea samples. The results from the qualitative test can be observed with the naked eye within 10 min, and from quantitative detection experiments the linear detection ranges for cabbage, parsley, spinach, and green tea samples were 2.3-122.0, 1.5-98.2, 2.1-145.5, and 6.6-129.7 ng g, respectively. Furthermore, recovery experiments were carried out at three spiked concentrations of LCT (5, 20, and 80 ng g), and the recoveries of the ICS in vegetable samples ranged from 81.2% to 96.7%, with a coefficient of variation (CV) of less than 8.1%. For green tea, the recoveries of the ICS were from 80.4% to 90.7%, with a CV of less than 8.7%. The ICS assay established in this study can be used for the qualitative and quantitative determination of LCT residues in foods and agricultural products.
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http://dx.doi.org/10.1039/d1ay00609fDOI Listing
July 2021

Changing light promotes isoflavone biosynthesis in soybean pods and enhances their resistance to mildew infection.

Plant Cell Environ 2021 Aug 21;44(8):2536-2550. Epub 2021 Jun 21.

Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, Sichuan, China.

Mildew severely reduces soybean yield and quality, and pods are the first line of defence against pathogens. Maize-soybean intercropping (MSI) reduces mildew incidence on soybean pods; however, the mechanism remains unclear. Changing light (CL) from maize shading is the most important environmental feature in MSI. We hypothesized that CL affects isoflavone accumulation in soybean pods, affecting their disease resistance. In the present study, shading treatments were applied to soybean plants during different developmental stages according to various CL environments under MSI. Chlorophyll fluorescence imaging (CFI) and classical evaluation methods confirmed that CL, especially vegetative stage shading (VS), enhanced pod resistance to mildew. Further metabolomic analyses and exogenous jasmonic acid (JA) and biosynthesis inhibitor experiments revealed the important relationship between JA and isoflavone biosynthesis, which had a synergistic effect on the enhanced resistance of CL-treated pods to mildew. VS promoted the biosynthesis and accumulation of constitutive isoflavones upstream of the isoflavone pathway, such as aglycones and glycosides, in soybean pods. When mildew infects pods, endogenous JA signalling stimulated the biosynthesis of downstream inducible malonyl isoflavone (MIF) and glyceollin to improve pod resistance.
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http://dx.doi.org/10.1111/pce.14128DOI Listing
August 2021

YY1-mediated reticulocalbin-2 upregulation promotes the hepatocellular carcinoma progression via activating MYC signaling.

Am J Cancer Res 2021 15;11(5):2238-2251. Epub 2021 May 15.

Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University Wuhan 430071, Hubei, People's Republic of China.

Hepatocellular carcinoma (HCC) is a common digestive tumor with high fatality worldwide. Previous studies have shown that Reticulocalbin-2 (RCN2) was a crucial factor for HCC proliferation, but invasion and migration mechanism of RCN2 contributing to HCC is poorly investigated. In this study, we estimated the RCN2 expression in both patient tissues and cell lines by polymerase chain reaction (PCR) and western blotting (WB), as well as the clinical information of HCC patients from public databases. Biological function induced by RCN2 in and was also researched through multiple functional experiments. Upstream and downstream signal of RCN2 was identified by bioinformatics. We found that up-regulated RCN2 was related to poorer prognosis in HCC patients and attached significance to HCC proliferation, invasion and migration. Luciferase reporter assay and chromatin immunoprecipitation validated that YY1 as the upstream transcription factor of RCN2, facilitating the expression of RCN2. Gene set enrichment analysis indicated that HCC progression induced by RCN2 might be related to MYC signaling. Furthermore, we demonstrated RCN2 reduced proteasomal degradation of MYC and lead to HCC progression. The effects of overexpressed RCN2 in HCC were attenuated by MYC silencing. In conclusion, our study highlighted the vital role of RCN2 in tumor progression and the potential benefit for the treatment of HCC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8167676PMC
May 2021

Transcriptional Responses of Interacted with Soybean to Cause Root Rot.

J Fungi (Basel) 2021 May 27;7(6). Epub 2021 May 27.

College of Agronomy & Sichuan Engineering Research Center for Crop Strip Intercropping System, Sichuan Agricultural University, Chengdu 611130, China.

is the most devastating pathogen of head blight of cereals, stalk and ear of maize, and it has recently become a potential threat for soybean as maize-soybean strip relay intercropping is widely practiced in China. To elucidate the pathogenesis mechanism of on intercropped soybean which causes root rot, transcriptional profiling of at 12, 24, and 48 h post-inoculation (hpi) on soybean hypocotyl tissues was conducted. In total, 2313 differentially expressed genes (DEGs) of were annotated by both KEGG pathway and Gene Ontology (GO) analysis. Among them, 128 DEGs were commonly expressed at three inoculation time points while the maximum DEGs were induced at 24 hpi. In addition, DEGs were also rich in carbon metabolism, ribosome and peroxisome pathways which might contribute to carbon source utilization, sexual reproduction, virulence and survival of when infected on soybean. Hence, this study will provide some basis for the deep understanding the pathogenesis mechanism of on different hosts and its effective control in maize-soybean strip relay intercropping systems.
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http://dx.doi.org/10.3390/jof7060422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227214PMC
May 2021

Investigation of hierarchically porous zeolitic imidazolate frameworks for highly efficient dye removal.

J Hazard Mater 2021 09 5;417:126011. Epub 2021 May 5.

Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; South China Institute of Collaborative Innovation, Dongguan 221116, China. Electronic address:

Treatment of textile water containing organic molecules as contaminants still remains a challenge and has become a central issue for environment remediation. Here, a nucleotide incorporated zeolitic imidazolate frameworks (NZIF) featuring hierarchically porous structure served as a potential adsorbent for removal of organic dye molecules. Adsorption isotherms of organic dyes were accurately described by Langmuir adsorption model with correlation coefficients of 0.98 and kinetic data followed the pseudo-second-order model. The maximum adsorption capacity of NZIF for Congo red (CR) and methylene blue (MB) reached 769 and 10 mg/g, respectively, which were 6 and 5 times higher than that of ZIF-8. The adsorption behavior of sunset yellow and crystal violet was examined for mechanism investigation. Analysis of pore size, molecular size, zeta potential and FTIR measurement together revealed that mesopores in NZIF provided more interaction sites and led to enhanced adsorption capacity. Hydrogen bonding and π-π stacking which resulted from the interaction between introduced nucleotide monophosphate and dyes dominated the driving forces for adsorption, where electrostatic interaction was also involved. Moreover, the introduced nucleoside monophosphate enabled NZIF to function under acidic condition whereas ZIF-8 collapsed. This study opens a new avenue for design of porous materials for environment remediation.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126011DOI Listing
September 2021

Potential Environmental Health Risk Analysis of Neonicotinoids and a Synergist.

Environ Sci Technol 2021 06 13;55(11):7541-7550. Epub 2021 May 13.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

The extensive use of neonicotinoid pesticides has led to their widespread presence in the environment, resulting in considerable safety risks to the ecosystem and human health. In this study, we investigated the biotransformation behavior of a cocktail of multiple neonicotinoids and piperonyl butoxide (PBO) synergist and their potential environmental health risk. It was found that neonicotinoids with a cyano group, such as acetamiprid and thiacloprid, tended to accumulate in liver and spleen tissues, while others with nitro groups (imidacloprid, thiamethoxam, clothianidin, dinotefuran, and nitenpyram) were mostly excreted in urine. In the presence of the synergist PBO, the metabolism of neonicotinoids changed, mainly through the nitro reduction pathway, while a low abundance of related metabolites was observed in the conventional hydroxylation and demethylation metabolic pathways, due to inhibition of CYP450 enzymes by the synergist. Furthermore, DNA methylation damage was exacerbated by the induction of hydroxylamine metabolites formed in the intermediate process of neonicotinoid metabolism with the synergistic effect of PBO, which resulted in a higher level of the O-methyldeoxyguanosine (O-medG) biomarker in the liver. Therefore, during the comprehensive evaluation of pesticide environmental risks, attention should be paid not only to the co-exposure mode under real environmental conditions but also to the potential risks of intermediate metabolism and related intermediate metabolites. This study provides a referential strategy and theoretical support for the health risk assessment of co-exposure of chemicals.
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http://dx.doi.org/10.1021/acs.est.1c00872DOI Listing
June 2021

Porcine Acellular Dermal Matrix Increases Fat Survival Rate after Fat Grafting in Nude Mice.

Aesthetic Plast Surg 2021 10 6;45(5):2426-2436. Epub 2021 May 6.

Department of Burn and Plastic Surgery, Second People's Hospital of Shenzhen, First Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, 518000, Guangdong, China.

Background: Autologous fat grafts have been widely in use for reconstruction, contour abnormalities, and cosmetic surgeries. However, the grafted fat one-year survival rate is unpredictable and always low (20%-80%). Standardizing the existing transplantation technology is difficult due to the limiting conditions. Scaffold materials or drugs are unsuitable to employ because of legal restrictions, complex production, and undetermined hazards. Therefore, a simpler and more effective approach to improve grafted fat survival rate is using commercial products as additives. Earlier studies proved that porcine acellular dermal matrix (PADM), a biomaterial clinically used for wound repair, could work as a scaffold for lipo-implantation. This study aimed at investigating the hitherto unclear effect of PADM on transplanted fat survival.

Methods: Thirty-two 8-week-old female nude mice were divided into two groups. Control mice received a 300 μl fat injection, while the PADM group mice were injected with a 300 μl PADM-fat mixture. After a 4-week treatment, fat weight and liquefaction ratio were assessed. Histological changes were quantified via hematoxylin & eosin (H&E) staining. Macrophage infiltration and vascular regeneration were revealed using an anti-CD34 antibody. Mouse and human mRNA expression levels were gauged via RNA-sequencing. On the third day post implantation, the mRNA expression levels of inflammatory genes Mcp-1 and Tnf-α were measured by qRT-PCR.

Results: The weight of surviving grafted fat did not differ between the control and the PADM group. However, adding PADM significantly decreased fat liquefaction. H&E-stained sections showed that PADM decreased fat necrosis, increased fat tissue regeneration, and raised CD34 levels in the regenerated tissue. RNA-sequencing showed that, compared to controls, fats from PADM-added group expressed more mouse-related mRNA but less human-related mRNA. The following GO and KEGG analysis showed that added PADM increased extracellular matrix (ECM) genes expression levels. The qRT-PCR showed that adding PADM increased Mcp-1 and Tnf-α mRNA expression levels.

Conclusions: In summary, PADM addition increased fat survival rate by reducing fat liquefaction through an increased macrophage infiltration, ECM regeneration, and revascularization. Therefore, PADM addition is a workable application in autologous fat grafting.

No Level Assigned: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02299-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481189PMC
October 2021

Serum deprivation-response protein induces apoptosis in hepatocellular carcinoma through ASK1-JNK/p38 MAPK pathways.

Cell Death Dis 2021 04 30;12(5):425. Epub 2021 Apr 30.

Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China.

Serum deprivation-response protein (SDPR), a phosphatidylserine-binding protein, which is known to have a promising role in caveolar biogenesis and morphology. However, its function in hepatocellular carcinoma (HCC) was still largely unknown. In this study, we discussed the characterization and identification of SDPR, and to present it as a novel apoptosis candidate in the incidence of HCC. We identified 81 HCC cases with lower SDPR expression in the tumor tissues with the help of qRT-PCR assay, and lower SDPR expression was potentially associated with poor prognostication. The phenotypic assays revealed that cell proliferation, invasion, and migration were profoundly connected with SDPR, both in vivo and in vitro. The data obtained from the gene set enrichment analysis (GSEA) carried out on the liver hepatocellular carcinoma (LIHC), and also The Cancer Genome Atlas (TCGA) findings indicated that SDPR was involved in apoptosis and flow cytometry experiments further confirmed this. Furthermore, we identified the interaction between SDPR and apoptosis signal-regulating kinase 1 (ASK1), which facilitated the ASK1 N-terminus-mediated dimerization and increased ASK1-mediated signaling, thereby activating the JNK/p38 mitogen-activated protein kinases (MAPKs) and finally enhanced cell apoptosis. Overall, this work identified SDPR as a tumor suppressor, because it promoted apoptosis by activating ASK1-JNK/p38 MAPK pathways in HCC.
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http://dx.doi.org/10.1038/s41419-021-03711-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087765PMC
April 2021

Drug-coated balloon angioplasty versus balloon angioplasty for treating patients with in-stent restenosis in the femoropopliteal artery: A meta-analysis.

Medicine (Baltimore) 2021 Apr;100(16):e25599

Department of Geriatrics, Wuhan Central Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: The introduction of endovascular surgery has led to frequent stent use, although in-stent restenosis (ISR) remains a challenging issue. Drug-coated balloon (DCB) and conventional balloon angioplasty (BA) are common endovascular procedures for addressing ISR in the femoropopliteal artery. However, there is controversy regarding which procedure provides the greatest benefit to patients.

Methods: The PubMed, EMBASE, and Cochrane Central Register of Controlled Trials databases were searched for prospective controlled trials that compared DCB and BA for patients with ISR in the femoropopliteal artery. The study has been approved by Ethics Committee of Wuhan Central Hospital.

Results: The meta-analysis included 6 prospective trials with 541 patients. We found that DCB use was associated with significant reductions in binary restenosis at 6 months (relative risk [RR]: 0.45, 95% confidence interval [CI]: 0.33-0.63; P < .00001), binary restenosis at 1 year (RR: 0.44, 95% CI: 0.34-0.57; P < .00001), target lesion revascularization (TLR) at 6 months (RR: 0.36, 95% CI: 0.20-0.65; P = .0006), and TLR at 1 year (RR: 0.38, 95% CI: 0.27-0.54; P < .00001). The DCB group also had significantly better clinical improvement (RR: 1.39, 95% CI: 1.13-1.71; P = .002), although we did not detect inter-group differences in terms of death, target vessel thrombosis, or ipsilateral amputation. The brand of DCB may a cause of heterogeneity.

Conclusion: Relative to BA, DCB use increases the durability of treatment for ISR in the femoropopliteal artery, based on significant reductions in binary restenosis and TLR at 6-12 months after the procedure. Furthermore, DCB use was associated with better clinical improvement. However, additional randomized controlled trials are needed to validate these findings.
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http://dx.doi.org/10.1097/MD.0000000000025599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078449PMC
April 2021

Autophagy in the HTR-8/SVneo Cell Oxidative Stress Model Is Associated with the NLRP1 Inflammasome.

Oxid Med Cell Longev 2021 27;2021:2353504. Epub 2021 Mar 27.

Department of Traditional Chinese Medicine, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

We investigated whether there was activation of NLRP1 inflammasomes and excessive autophagy in oxidative stress damage. And we further demonstrate whether there is a cascade relationship between the activation of NLRP1 inflammasomes and the phenomenon of excessive autophagy. To observe the expression level of the NLRP1 inflammasome group in the pathological process of trophoblast cell oxidative stress, western blot, immunofluorescence, and qRT-PCR were performed. Autophagy in trophoblast cells after the action of HO was detected by using normal trophoblast cells' NLRP1-specific activator (MDP) as a positive control. The presence of excessive autophagy was determined by comparing it with the autophagy-related proteins in normal trophoblast cells. Through siRNA-NLRP1, we investigated the role of oxidative stress and the NLRP1 inflammasome in autophagy in cells. 100 mol MDP for 24 hours can be used as the optimal concentration of the NLRP1 activator. In human placental trophoblast oxidative stress, the model group significantly increased the expression level of inflammasome IL-1, CASP1, and NLRP1, compared with the control group NLRP3, and LC3-II, Beclin-1, ATG5, ATG7, and p62 overactivated the autophagy ability of cells. After the activation of NLRP1, the expression of these inflammasomes increased, accompanied by the decrease in autophagy. After the expression of NLRP1 was silenced by RNAi, the expression of inflammasome IL-1, CASP1, and NLRP3 was also decreased. Still, the autophagy level was increased, which was manifested by the high expression of LC3-II, Beclin-1, ATG5, and ATG7 and the decrease in p62. Trophoblast cells showed the expression of NLRP1 protein and excessive autophagy under oxidative stress. Simultaneously, the NLRP1 inflammasome of trophoblast cells in the state of oxidative stress was correlated with autophagy. Inflammasome activation and autophagy were shown to be linked and to influence each other mutually. These may also provide new therapeutic targets in a pathological pregnancy.
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http://dx.doi.org/10.1155/2021/2353504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019638PMC
June 2021

A Versatile Competitive Coordination Strategy for Tailoring Bioactive Zeolitic Imidazolate Framework Composites.

Small 2021 Apr 6:e2007586. Epub 2021 Apr 6.

Lab of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, No. 381 Wushan Road, Guangzhou, Guangdong, 510640, China.

Zeolitic imidazolate frameworks (ZIFs) serving as platforms for bioactive guest encapsulation have attracted growing attention, yet the tailoring of its architectures and bioactivity remains a major challenge. Herein, a versatile competitive coordination strategy is proposed by using amorphous zinc nucleotide gel as template for step-by-step growth of ZIFs, which enables the tailoring of bioactive ZIF composites under facile conditions. Mechanism investigation reveals that introduced nucleotide determines the hierarchical pore structure and hydrophilicity, leading to customized activity retention and stability of the resultant bioactive ZIF composites. Furthermore, nucleoside monophosphate enhances the acidic tolerance of ZIFs. To the authors' knowledge, this is the first example showing the dynamic evolution of amorphous gels to crystalline ZIFs for in situ encapsulation of enzymes with tailored catalytic performance. This study provides insights for rational design of ZIF-based biocomposites and broadens the application of bioactive metal-organic frameworks.
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http://dx.doi.org/10.1002/smll.202007586DOI Listing
April 2021

MiR-1179 is downregulated in cervical cancer and its overexpression suppresses cancer cells invasion by targeting CHAF1A/ZEB1.

Acta Biochim Pol 2021 Mar;68(2):193-199

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710004, China.

The anticancer effect of miR-1179 has been extensively studied in many tumors. The mechanism of miR-1179 action in cervical cancer, however, remains largely unknown. In the present study, miR-1179 was downregulated in both cervical cancer cell lines and cancer tissues. In addition, miR-1179 mimic suppressed cancer cells invasion and epithelial-mesenchymal transition (EMT) in cervical cancer SiHa and Caski cells. We found that chromatin assembly factor 1 subunit A (CHAF1A) might be a direct target of miR-1179 and could be regulated by miR-1179. Furthermore, CHAF1A shRNA suppressed the cervical cancer cells invasion and the expression of EMT-promoted proteins. Reversely, CHAF1A overexpression not only promoted cervical cancer cells invasion but also upregulated the level of Zinc finger E-box binding protein 1 (ZEB1), an EMT-related protein. The induction of ZEB1 could be counteracted by miR-1179 overexpression. It was observed that in cervical cancer patients' tissues, miR-1179 was downregulated while the pathway of CHAF1A/ZEB1 was upregulated. In summary, our research indicated that the miR-1179 might regulate CHAF1A/ZEB1 axis and inhibit the invasion of cervical cancer cells.
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http://dx.doi.org/10.18388/abp.2020_5499DOI Listing
March 2021

Exposure to parabens and associations with oxidative stress in adults from South China.

Sci Total Environ 2021 Jun 3;774:144917. Epub 2021 Feb 3.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address:

Parabens are widely applied as preservatives in cosmetics, drugs and food. Previous studies suggested that parabens could exhibit potential risks to human health. However, data on human exposure levels and health effects of parabens remain limited, especially in the potential effects on DNA oxidative stress. This study aimed to investigate urinary levels of parabens in adults from South China and explore the relationships between urinary parabens and DNA oxidative stress. Five short chain parabens, including methyl paraben (MeP), ethyl paraben (EtP), n-propyl paraben (PrP), butyl paraben (BuP) and benzyl paraben (BzP), were determined in urine from 319 adults in Shenzhen, China. MeP, EtP and PrP were frequently detected in urine samples (detection frequencies >66.5%), suggesting broad exposure in South China adults. Median concentrations of MeP, EtP, PrP, BuP and BzP were 5.78, 0.39, 0.35, 0.01 and 0.02 μg/L, respectively. A significantly positive correlation was observed between the urinary concentrations of MeP and PrP (p < 0.01), suggesting similar sources for these two chemicals. In addition, participants with alcohol consumption exhibited significantly lower paraben concentrations in urine than those without alcohol drinking (p < 0.05). Significant association was observed between urinary concentrations of parabens and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels (p < 0.01), while no significant dose-response relationship was found (p > 0.05). A potential risk from PrP exposure was found in South China adults.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144917DOI Listing
June 2021

Self-limiting self-assembly of supraparticles for potential biological applications.

Nanoscale 2021 Feb;13(4):2302-2311

International Joint Research Laboratory for Biointerface and Biodetection, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China and State Key Laboratory of Food Science and Technology, Jiangnan University, Jiangsu, People's Republic of China.

Nanotechnology has largely spurred the development of biological systems by taking advantage of the unique chemical, physical, optical, magnetic, and electrical properties of nanostructures. Self-limiting self-assembly of supraparticles produce new nanostructures and display great potential to create biomimicking nanostructures with desired functionalities. In this minireview, we summarize the recent developments and outstanding achievements of colloidal supraparticles, such as the driving forces for self-limiting self-assembly of supraparticles and properties of constructed supraparticles. Their application values in biological systems have also been illustrated.
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http://dx.doi.org/10.1039/d0nr08001bDOI Listing
February 2021

Post-surgery anxiety and depression in prostate cancer patients: prevalence, longitudinal progression, and their correlations with survival profiles during a 3-year follow-up.

Ir J Med Sci 2021 Nov 7;190(4):1363-1372. Epub 2021 Jan 7.

Department of Intensive Care Unit, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 26 Shengli Street, Wuhan, 430014, China.

Background: Anxiety and depression are more frequent in cancer patients than general population and may be correlated with cancer prognosis; however, their value in prostate cancer patients is largely unknown. We aimed to evaluate prevalence of anxiety and depression in prostate cancer survivors post the surgeries, and their correlations with patients' disease-free survival (DFS) and overall survival (OS).

Methods: A hundred and ninety-four patients with prostate cancer who underwent radical prostatectomy were enrolled. After discharged from hospital, patients were assessed for post-surgery anxiety and depression every 3 months using Zung Self-rating Anxiety/Depression Scale (SAS/SDS) for a total of 36 months. In addition, disease conditions, DFS, and OS were also documented.

Results: SAS score (P < 0.001), anxiety rate (P = 0.004), SDS score (P < 0.001), and depression rate (P < 0.001) gradually elevated from baseline to month 36 in prostate cancer patients. Anxiety at baseline (P = 0.009) and anxiety at 3 years (P = 0.017) were correlated with worse DFS, and anxiety at baseline (P = 0.009) was also correlated with shorter OS in prostate cancer patients. Furthermore, depression at baseline (P = 0.005) and depression at 2 years (P = 0.008) were associated with unfavorable DFS, and depression at baseline (P = 0.001), 1 year (P = 0.025), and 2 years (P = 0.008) were associated with worse OS in prostate cancer patients. Moreover, multivariate Cox's proportional hazards regression analysis elucidated that depression at baseline (P = 0.027) was an independent predictive factor for shorter DFS in prostate cancer patients.

Conclusion: Anxiety and depression both gradually deteriorate, and they correlate with unfavorable survival profile in prostate cancer patients after radical prostatectomy.
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http://dx.doi.org/10.1007/s11845-020-02417-xDOI Listing
November 2021

A novel long non-coding RNA RP11-286H15.1 represses hepatocellular carcinoma progression by promoting ubiquitination of PABPC4.

Cancer Lett 2021 02 28;499:109-121. Epub 2020 Nov 28.

Department of Hepatobiliary and Pancreatic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, PR China. Electronic address:

Hepatocellular carcinoma (HCC) is a malignancy found at high frequency around the world. Unfortunately, the scarcity of effective early diagnostic methods invariably results in poor outcomes. Long noncoding RNAs (lncRNAs) are known to regulate the progression of hepatocellular carcinoma (HCC). A novel lncRNA RP11-286H15.1(OTTHUMG00000186042) has been identified and associated with HCC; however, the potential role of RP11-286H15.1 in HCC remains undefined. The transcript abundance of RP11-286H15.1 in 80 pairs of HCC samples and cell lines was evaluated by qRT-PCR analysis. The functional role of RP11-286H15.1 in HCC was tested in vivo and in vitro. The mechanisms underlying the role of RP11-286H15.1 in HCC were explored by RNA pulldown, transcriptome sequencing, and RNA immunoprecipitation (RIP), ubiquitination and fluorescence in situ hybridization (FISH) assays as well as Western blot analysis. The qRT-PCR and FISH assays revealed that RP11-286H15.1 was significantly decreased in HCC, and implied a shorter survival time. RP11-286H15.1 overexpression inhibited HCC cell proliferation and metastasis in vitro and in vivo, whereas RP11-286H15.1 knockdown produced the opposite results. Furthermore, we confirmed that RP11-286H15.1 (620-750 nucleotides) binds to poly(A) binding protein 4 (PABPC4) and promotes its ubiquitination, thus, reducing the stability of TRIM37 and CDC27 mRNAs. Our study demonstrates that a novel lncRNA, RP11-286H15.1, represses HCC progression by promoting PABPC4 ubiquitination. These findings highlight potential therapeutic targets for HCC.
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http://dx.doi.org/10.1016/j.canlet.2020.11.038DOI Listing
February 2021

Impact of knockdown LincRNA-Cox2 on apoptosis of macrophage infected with Bacillus Calmette-Guérin.

Mol Immunol 2021 02 26;130:85-95. Epub 2020 Nov 26.

Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, NingXia University, NingXia, Yinchuan, 750021, China; School of Life Science, NingXia University, NingXia, Yinchuan, 750021, China. Electronic address:

Mycobacterium tuberculosis (Mtb)-induced apoptosis of alveolar macrophages plays an important role in the pathogenesis of tuberculosis. Previous studies indicated that massive LncRNAs could deteriorate MTB invasion or latent infection by regulating macrophage's apoptosis. However, whether LincRNA-Cox2 is involved in apoptosis of macrophage infected with Mtb is unclear. In this study, we found Bacillus Calmette-Guerin(BCG)infection induced cell apoptosis with a increasing LincRNA-Cox2 expression in RAW264.7 cells. Furthermore, the activation of TLR signal pathway elevated the expression of lincRNA-Cox2. In this regard, we used small interfering RNA to explore the role of LincRNA-Cox2 on regulating apoptosis of RAW264.7 cells infected with BCG. The results showed that si-LincRNA-Cox2 was capable of increased the expression of apoptosis-associated proteins and accumulation of ROS in BCG-infected RAW264.7 cells. Mechanically, si-LincRNA-Cox2 facilitated BCG-induced macrophage apoptosis by activating the intrinsic apoptotic pathway as well as increased the genes expression of PERK/eIF2α/CHOP. These results provide novel insights into host-pathogen interactions and highlight the potential role of LincRNA-Cox2 in regulating apoptosis induced by BCG-infection.
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http://dx.doi.org/10.1016/j.molimm.2020.11.008DOI Listing
February 2021

Protein A immunoadsorption combination with immunosuppressive therapy improves neuropsychiatric systemic lupus erythematosus: A case report.

Clin Case Rep 2020 Nov 16;8(11):2158-2162. Epub 2020 Jul 16.

Department of Nephrology Guangdong Provincial People's Hospital Guangdong Academy of Medical Sciences Guangzhou China.

We described protein A immunoadsorption combination with immunosuppressive treatment improved rapidly a patient with Neuropsychiatric systemic lupus erythematosus.
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http://dx.doi.org/10.1002/ccr3.3140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669424PMC
November 2020
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