Publications by authors named "Xiaoliang Chen"

122 Publications

Associations Between Child Maltreatment and Depressive Symptoms Among Chinese College Students: An Analysis of Sex Differences.

Front Psychiatry 2021 9;12:656646. Epub 2021 Jul 9.

Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Depressive symptoms and child maltreatment are both global public health problems among young adults. This study aimed to investigate the associations between five types of child maltreatment and depressive symptoms among Chinese college students, with a focus on potential sex differences. A cross-sectional study of a nationally representative sample of Chinese college students was conducted from March to June 2019 with a multistage, stratified cluster, random sampling method. In total, 30,179 college students from 60 colleges of 10 Chinese province-level regions completed standard questionnaires, including a history of child maltreatment and current depressive symptoms. The prevalence of depressive symptoms among college students in China was 7.3%. After adjusting for control variables, physical abuse (adjusted odds ratio [aOR] = 1.20, 95% confidence interval [CI] = 1.17-1.23), emotional abuse (aOR = 1.21, 95% CI = 1.19-1.23), sexual abuse (aOR = 1.19, 95% CI = 1.16-1.22), physical neglect (aOR = 1.14, 95% CI = 1.12-1.16) and emotional neglect (aOR = 1.08, 95% CI = 1.07-1.09) were all positively associated with depressive symptoms. Notably, a cumulative effect of child maltreatment on depressive symptoms among Chinese college students was observed. Moreover, sex differences in the associations of emotional abuse, emotional neglect, and the number of maltreatment types with depressive symptoms were statistically significant ( < 0.05). Further stratification analyses showed that female students who experienced emotional abuse and emotional neglect had a higher risk of depressive symptoms than male students, and the cumulative effect of maltreatment types was stronger for females than males. Five types of child maltreatment and their co-occurrence were associated with an increased risk of depressive symptoms among college students. Furthermore, the effects of emotional abuse, emotional neglect and the number of maltreatment types on depressive symptoms were stronger for females than for males. These findings can promote understanding of the effects of child maltreatment on depressive symptoms, and prevention and intervention strategies for depressive symptoms should consider the type of child maltreatment and sex differences.
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http://dx.doi.org/10.3389/fpsyt.2021.656646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298832PMC
July 2021

The diagnostic value of metabolic, morphological and heterogeneous parameters of 18F-FDG PET/CT in mediastinal lymph node metastasis of non-small cell lung cancer.

Nucl Med Commun 2021 Jul 15. Epub 2021 Jul 15.

Department of Nuclear Medicine Department of Radiology, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing 400030, P.R. China.

Objectives: To investigate the value of PET/CT metabolic, morphological and heterogeneous parameters in the diagnosis of 18F-FDG positive mediastinal lymph node metastasis in non-small cell lung cancer (NSCLC).

Patients And Methods: A total of 156 patients with pathologically diagnosed NSCLC and underwent 18F-FDG PET/CT scans were enrolled in this study. Mediastinal lymph nodes with 18F-FDG uptake greater than the mediastinum were analyzed. The metabolic parameters of maximum and mean standardized uptake value (SUVmax, SUVmean), SUVratio (node SUVmax/mediastinum SUVmax), metabolic tumor volume (MTV), total lesion glycolysis (TLG), morphological parameters of maximum short diameter (Dmin), CT values and metabolic heterogeneity parameter of coefficient of variation (COV) were measured. The performance of each parameter and their combinations for diagnosis of lymph node metastasis was evaluated through receiver operating characteristic (ROC) curves and binary logistic regression analysis.

Results: There were 206 lymph nodes with pathological evidence included in the study, including 103 metastatic and 103 nonmetastatic nodes. The SUVmax, SUVmean, SUVratio, TLG, COV and Dmin of metastatic lymph nodes were significantly higher/ greater than those in nonmetastatic ones (P < 0.05). ROC curve analysis revealed that the combination of SUVratio, Dmin and COV showed the highest diagnostic efficacy among all single and combined parameters, the area under the curve (AUC) was 0.907 (P = 0.000), these three parameters all increased the risk of lymph node metastasis, with odds ratios of 1.848, 1.293 and 1.258, respectively (all P < 0.05).

Conclusion: Heterogeneity parameter was helpful for the accurate distinction of mediastinal lymph node metastasis in NSCLC. The combination of the SUVratio, Dmin and COV could improve the diagnostic accuracy. Multiple-parameters analysis plays an important complementary role in the diagnosis of lymph node metastasis.
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http://dx.doi.org/10.1097/MNM.0000000000001456DOI Listing
July 2021

Channel-Crack-Designed Suspended Sensing Membrane as a Fully Flexible Vibration Sensor with High Sensitivity and Dynamic Range.

ACS Appl Mater Interfaces 2021 Jul 16;13(29):34637-34647. Epub 2021 Jul 16.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi 710049, China.

Vibration sensors are essential for signal acquisition, motion measuring, and structural health evaluations in civil and industrial applications. However, the mechanical brittleness and complicated installation process of micro-electromechanical system vibration sensors block their applications in wearable devices and human-machine interaction. The development of flexible vibration sensors satisfying the requirements of good flexibility, high sensitivity, and the ability to attach conformably on curved critical components is highly demanded but still remains a challenge. Here, we demonstrate a highly sensitive and fully flexible vibration sensor with a channel-crack-designed suspended sensing membrane for high dynamic vibration and acceleration monitoring. The flexible sensor is designed as a suspended vibration membrane structure by bonding a channel-crack-sensing membrane on a cavity substrate, of which the suspended sensing membrane can freely vibrate out of plane under external vibration. By inducing the cracks to be generated in the embedded multiwalled carbon nanotube channels and fully cracked across the conducting routes, the suspended vibration membrane shows high sensitivity, good reproducibility, and robust sensing stability. The resultant vibration sensor demonstrates an ultrawide frequency vibration response range from 0.1 to 20,000 Hz and exhibits the ability to respond to acceleration vibration with a broad response of 0.24-100 m/s. The high sensitivity, wide bandwidth, and fully flexible format of the vibration sensor enable it to be directly attached on human bodies and curvilinear surfaces to conduct in situ vibration sensing, which was demonstrated by motion detection, voice identification, and the vibration monitoring of mechanical equipment.
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http://dx.doi.org/10.1021/acsami.1c09963DOI Listing
July 2021

Nanoscopic Imaging of Nucleolar Stress Enabled by Protein-Mimicking Carbon Dots.

Nano Lett 2021 07 28;21(13):5689-5696. Epub 2021 Jun 28.

State Key Laboratory of Heavy Oil Processing and College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

The nucleolus is a central hub for coordinating cellular stress responses during cancer development and treatment. Accurate identification of nucleolar stress response is crucially desired for nucleolus-based diagnostics and therapeutics but technically challenging due to the need to address the ultrastructural analysis. Here, we report a protein-like CD with the integration of fluorescent blinking domains and RNA-binding motifs, which offers the ability to perform enhanced super-resolution imaging of the nucleolar ultrastructure. This image allows extraction of multidimensional information from the nucleolus for accurate distinguishment of different cells from the same cell types. Furthermore, we demonstrate for the first time this CD-depicted nucleolar ultrastructure as a sensitive hallmark to identify and discriminate subtle responses to various stressors as well as to afford RNA-related information that has been inaccessible by conventional immunofluorescence methods. This protein-mimicking CD could become a broadly useful probe for nucleolar stress studies in cell diagnostics and therapeutics.
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http://dx.doi.org/10.1021/acs.nanolett.1c01420DOI Listing
July 2021

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Oncogene 2021 Jul 23;40(30):4884-4893. Epub 2021 Jun 23.

State Key Laboratory of Oncogenes and Related Genes, Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Pancreatic ductal adenocarcinoma (PDAC) is the deadliest cancer mainly owing to its proclivity to early metastasis and the lack of effective targeted therapeutic drugs. Hence, understanding the molecular mechanisms underlying early invasion and metastasis by PDAC is imperative for improving patient outcomes. The present study identified that upregulation of TSPAN8 expression in PDAC facilitates metastasis in vivo and in vitro. We found SOX9 as a key transcriptional regulator of TSPAN8 expression in response to EGF stimulation. SOX9 modulation was sufficient to positively regulate endogenous expression of TSPAN8, with concomitant in vitro phenotypic changes such as loss of cell-matrix adherence and increased invasion. Moreover, increased SOX9 and TSPAN8 levels were shown to correlate in human pancreatic cancer specimens and downregulated in vitro by EGFR tyrosine kinase inhibitors. High expression of SOX9 and TSPAN8 has been associated with tumor stage, poor prognosis and poor patient survival in PDAC. In conclusion, this study highlights the importance of the EGF-SOX9-TSPAN8 signaling cascade in the control of PDAC invasion and implies that TSPAN8 may be a promising novel therapeutic target for the treatment of PDAC.
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http://dx.doi.org/10.1038/s41388-021-01864-9DOI Listing
July 2021

Tuning the Mechanical and Electrical Properties of Porous Electrodes for Architecting 3D Microsupercapacitors with Batteries-Level Energy.

Adv Sci (Weinh) 2021 Jun 20:e2004957. Epub 2021 Jun 20.

Micro-/Nano-technology Research Center, State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.

Microsupercapacitors (MSCs) are vital power sources for internet of things (IoTs) and miniaturized electronics. The performance of MSCs is often restricted by its low areal energy density, which is due to the low areal mass loading of active materials. Constructing thick planar microelectrode with fine structure and high aspect ratio is an efficient way to increase mass loading, but limited by the breakable nature of porous electrode materials. Here, it is found that the mechanical and electrical properties of porous electrodes, as well as their surface area utilization and internal ion diffusion pathway, can be synergistically tuned by infilling gel electrolyte into internal pores of porous electrode films. The tuned thick porous electrode films are robust enough to enable laser ablation of three dimensional (3D) microelectrodes for high mass loading and high aspect ratio. The areal capacitance of 3D microelectrodes is able to increase linearly with mass loading (or thickness) up to at least 13 mg cm  (or 260 µm) for a value of up to 4640 mF cm based on active carbon. The 3D MSCs deliver areal energy density of 1318 μWh cm , which is comparable to the best of Li-ion 3D microbatteries while exhibiting superior electrochemical and mechanical stability.
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http://dx.doi.org/10.1002/advs.202004957DOI Listing
June 2021

Deep-Learning-Assisted Single-Molecule Tracking on a Live Cell Membrane.

Anal Chem 2021 Jun 16. Epub 2021 Jun 16.

State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, China.

Single-molecule fluorescence imaging is a powerful tool to study protein function by tracking molecular position and distribution, but the precise and rapid identification of dynamic molecules remains challenging due to the heterogeneous distribution and interaction of proteins on the live cell membrane. We now develop a deep-learning (DL)-assisted single-molecule imaging method that can precisely distinguish the monomer and complex for rapid and real-time tracking of protein interaction. This DL-based model, which comprises convolutional layers, max pooling layers, and fully connected layers, is trained to reach an accuracy of >98% for identifying monomer and complex. We use this method to investigate the dynamic process of chemokine receptor CXCR4 on the live cell membrane during the early signaling stage. The results show that, upon ligand activation, the CXCR4 undergoes a dynamic process of forming a receptor complex. We further demonstrate that the CXCR4 complex tends to be internalized at 2.5-fold higher rate into the cell interior than the monomer via the clathrin-dependent pathway. This study is the first example to scrutinize the early signaling process of CXCR4 at the single-molecule level on the live cell membrane. We envision that this DL-assisted imaging method would be a broadly useful technique to study more protein families for elucidating their physiological and pathological functions.
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http://dx.doi.org/10.1021/acs.analchem.1c00547DOI Listing
June 2021

Impact of variceal eradication on rebleeding and prognosis in cirrhotic patients undergoing secondary prophylaxis.

Ann Transl Med 2021 Apr;9(7):540

Department of Gastroenterology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Background: Endoscopic therapy has been widely applied to prevent variceal rebleeding, but data addressing the effect of endoscopic variceal eradication (VE) are lacking. We aimed to clarify the clinical impact of VE and reveal the long-term incidence and mortality of gastrointestinal rebleeding.

Methods: This prospective study included 228 cirrhotic patients who underwent secondary prophylaxis for variceal bleeding and achieved VE through a systematic procedure we proposed as endoscopic sequential therapy (EST). Rebleeding rates before and after VE were compared and cumulative incidence of rebleeding and mortality were calculated using the Kaplan-Meier method. A logistic regression model and P for trend were used to investigate the optimal time limit for VE.

Results: During a median (interquartile range) follow-up duration of 33.0 (23.0-48.75) months, rebleeding was identified in 28 patients (12.3%) after VE and in 27 patients (11.8%) during endoscopic sessions. The cumulative incidence of rebleeding before and after VE was 8.4% and 1.8% at 6 months, and 14.9% and 4.0% at 1 year respectively (P<0.001). The long-term incidence of all-cause/variceal rebleeding following VE was 10.4%/9.1%, and 31.5%/23.5% at 2 and 5 years respectively. Eleven patients (4.8%) died and the 5-year mortality was 9.3%. VE achieved within 6 months was associated with fewer rebleeding events compared to VE achieved after 6 months (5.5% . 20.0%, P=0.002), while logistic regression revealed an overall increasing trend in the odds ratio of rebleeding ( patients with VE time ≤6 months) for patients with 6< VE time ≤12 months and VE time >12 months (P for trend <0.001).

Conclusions: VE further reduces rebleeding based on routine endoscopic prophylaxis and improves long-term prognosis. VE within 6 months seems to be the optimal timing and should therefore be advocated.
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http://dx.doi.org/10.21037/atm-20-3401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105824PMC
April 2021

Nonblinking carbon dots for imaging and tracking receptors on a live cell membrane.

Chem Commun (Camb) 2021 Jun 10;57(45):5554-5557. Epub 2021 May 10.

State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266580, China.

Blinking occurs with nearly all fluorophores including organic dyes, fluorescent proteins, semiconductor quantum dots and carbon dots (CDs). We developed non-blinking and photoresistant fluorescent CDs by introducing multiple aromatic domains onto a single carbon dot and demonstrated their great potential for imaging and tracking of receptors on a live cell membrane.
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http://dx.doi.org/10.1039/d1cc01120kDOI Listing
June 2021

The relationship between childhood emotional abuse and depressive symptoms among Chinese college students: The multiple mediating effects of emotional and behavioral problems.

J Affect Disord 2021 06 31;288:129-135. Epub 2021 Mar 31.

Department of Psychiatry, University of Toronto, Toronto, ON, Canada.

Background: This study aims to explore the mediational effects of emotional and behavioral problems on the association between childhood emotional abuse and depressive symptoms among college students.

Methods: Data were drawn from 60 universities from 10 provinces in China (n=30,374). Information about childhood maltreatment, depressive symptoms, emotional and behavioral problems were gathered through the Childhood Trauma Questionnaire-Short Form (CTQ-SF), the Center for Epidemiologic Studies Depression Scale (CES-D) and the Strengths and Difficulties Questionnaire (SDQ), respectively. Univariable and multivariable logistic regression models and mediating models were used.

Results: After controlling for demographic factors, childhood emotional abuse was the strongest risk factor for depressive symptoms (adjusted odds ratio (aOR)=2.54, 95%CI=2.27-2.85). The relationship between childhood emotional abuse and depressive symptoms was partially mediated by emotional and behavioral problems with 68.7% total indirect effect. Among the 5 identified subtypes of emotional and behavioral problems, the mediating effects of emotional problems (57.3%) and hyperactivity (28.6%) were higher than peer problems (7.8%) and prosocial behavior (3.6%). Conduct problems did not show a significant mediating effect (p>0.05).

Limitations: The cross-sectional design is limited to make inferences about causality.

Conclusions: Childhood emotional abuse was strongly associated with depressive symptoms in college students. Of the five identified subtypes of emotional and behavioral problems, four subtypes mediated the relationship between childhood emotional abuse and depressive symptoms, including emotional problems, hyperactivity, peer problems and prosocial behavior.
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http://dx.doi.org/10.1016/j.jad.2021.03.074DOI Listing
June 2021

Poly-Adenine-Based Spherical Nucleic Acids for Efficient Live-Cell MicroRNA Capture.

Angew Chem Int Ed Engl 2021 06 24;60(26):14438-14445. Epub 2021 May 24.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai, 200240, China.

Direct delivery of exogenous non-coding nucleic acids into living cells has attracted intense interest in biological applications. However, the cell entry efficiency and target capture ability remain to be improved. Herein, we report a method for compartmenting the nucleic acids on the surface of poly-adenine-based spherical nucleic acids (polyA-SNAs) for efficient capture of oncogenic microRNAs (miRNAs) in living cells. We find that polyA-SNAs exhibit high cell entry efficiency, which is insensitive to the configuration of the anti-miRNA sequences. By programming the length of polyAs, we precisely engineered the spatial configuration of the anti-miRNA sequences in polyA-SNAs. Compartmentalized polyA-SNAs bind to miRNAs with improved capture ability as compared to densely compacted SNAs. We further demonstrate that polyA-SNAs serve as high-efficacy miRNA sponges for capturing oncogenic miRNAs both in living cells and in mice. The efficient inhibition of miRNAs results in significant suppression of tumor growth.
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http://dx.doi.org/10.1002/anie.202017039DOI Listing
June 2021

Using a multidisciplinary team for the staged management and optimally minimally invasive treatment of severe acute pancreatitis.

Biosci Trends 2021 Jul 11;15(3):180-187. Epub 2021 Apr 11.

Hepatobiliary Surgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, China.

Severe acute pancreatitis (SAP) is a common critical disease with a high mortality rate that involves a complex, rapid change in condition and multiple organ systems. Therefore, a multidisciplinary team (MDT), including staff from the emergency department, intensive care unit, pancreatic surgery, gastroenterology , and imaging, is necessary for the early diagnosis, evaluation, and treatment of patients with SAP. This involves managing the systemic inflammatory response and maintaining organ function in the early stage and managing systemic infection and treatment of peripancreatic complications in the middle-to-late stages. The MDT should be led by departments corresponding to the clinical characteristics of each stage, and those departments should be responsible for the coordination and implementation of treatment by other relevant departments. In the late stage, pancreatic surgery and gastroenterology are the main departments that should manage peripancreatic complications. In line with the principle of minimally invasive treatment, the timely and reasonable selection of endoscopic or minimally invasive surgical debridement can achieve good therapeutic outcomes. Open surgery is also an effective method for treating an intractable massive hemorrhage in the abdominal cavity or necrotic cavity, intractable abdominal compartment syndrome, visceral perforation, and fistulae.
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http://dx.doi.org/10.5582/bst.2021.01075DOI Listing
July 2021

Nanoimprinting metal-containing nanoparticle-doped gratings to enhance the polarization of light-emitting chips by induced scattering.

Nanotechnology 2021 Mar 19;32(23):235304. Epub 2021 Mar 19.

Micro- and Nano-technology Research Center, State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, People's Republic of China.

Polarized radiative luminous semiconductor chips have huge application potential in many highly value-added fields. The integration of a subwavelength grating is recognized to be the most promising method for the development of polarized chips, but still faces the challenge of low polarized radiative performance. This paper describes a proposal for, and the development of, a scattering-induced enhanced-polarization light-emitting diode chip by directly nanoimprinting a metal-containing nanoparticle-doped grating onto the top surface of a common flip chip. The rate at which quantum-well light emission is used by the developed polarized chip is improved by more than 30%. More attractively, the doped scattering nanoparticles function as a scattering-induced polarization state converter that is sandwiched in between the top aluminum grating and the bottom silver reflector of the chips. The originally non-radiated light, with an electric-field vector parallel to the grating lines, is reflected back and forth inside the sandwich until it changes to the perpendicular vibration mode and is radiated outside the chip. Therefore, the polarization extinction ratio is greatly improved, compared to undoped samples.
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http://dx.doi.org/10.1088/1361-6528/abe964DOI Listing
March 2021

Fabrication of ulcer-adhesive oral keratin hydrogel for gastric ulcer healing in a rat.

Regen Biomater 2021 Mar 13;8(2):rbab008. Epub 2021 Mar 13.

Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.

Hydrogel has been used for in suit gastric ulcer therapy by stopping bleeding, separating from ulcer from gastric fluids and providing extracellular matrix scaffold for tissue regeneration, however, this treatment guided with endoscopic catheter in most cases. Here, we developed an oral keratin hydrogel to accelerate the ulcer healing without endoscopic guidance, which can specially adhere to the ulcer because of the high-viscosity gel formation on the wound surface . Approximately 50% of the ulcer-adhesive keratin hydrogel can resident in ethanol-treated rat stomach within 12 h, while approximately 18% of them maintained in health rat stomach in the same amount of time. Furthermore, Keratin hydrogels accelerated the ethanol-induced gastric ulcer healing by stopping the bleeding, preventing the epithelium cells from gastric acid damage, suppressing inflammation and promoting re-epithelization. The oral administration of keratin hydrogel in gastric ulcer treatment can enhance the patient compliance and reduce the gastroscopy complications. Our research findings reveal a promising biomaterial-based approach for treating gastrointestinal ulcers.
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http://dx.doi.org/10.1093/rb/rbab008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955710PMC
March 2021

Nanoimprinting Metal-Containing Nanoparticles-Doping-Grating for High Polarization Performance Light Emitting Chips by Scattering-Induced Enhancement.

Nanotechnology 2021 Feb 24. Epub 2021 Feb 24.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, CHINA.

Polarized radiative semiconductor luminous chips have huge application potentials in lots of high value-added fields. Integrating a subwavelength grating is recognized as the most promising method for development of polarized chips, but still faces the challenge of lower polarized radiation performance. The paper proposed and developed a scattering-induced polarization enhancement light emitting diodes chips by directly nanoimprinting metal-containing nanoparticles-doping-grating on the top surface of a common flip-chips. The utilization rate of quantum well light emission on the developed polarized chips was improved more than 30%. More attractively, the doped scattering nanoparticles play as a scattering-induced polarization state converter that sandwiched in the top aluminum grating and bottom silver reflector on the chips. The originally non-radiative light with an electric field vector parallel to the grating lines is reflected back and forth among the sandwich until it changes to the perpendicular vibration mode to radiate outside the chips. Therefore, the polarization extinction ratio was greatly improved compared with the samples without doping.
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http://dx.doi.org/10.1088/1361-6528/abe964DOI Listing
February 2021

Carbon Dot Blinking Enables Accurate Molecular Counting at Nanoscale Resolution.

Anal Chem 2021 03 18;93(8):3968-3975. Epub 2021 Feb 18.

State Key Laboratory of Heavy Oil Processing and College of Chemical Engineering China, University of Petroleum (East China), Qingdao 266580, China.

Accurate counting of single molecules at nanoscale resolution is essential for the study of molecular interactions and distribution in subcellular fractions. By using small-sized carbon dots (CDs), we have now developed a quantitative single-molecule localization microscopy technique (qSMLM) based on spontaneous blinking to count single molecules with a localization precision of 10 nm, which can be accomplished on conventional microscopes without sophisticated laser control. We explore and adapt the blinking of CDs with diverse structures and demonstrate a counting accuracy of >97% at a molecular density of 500 per μm. When applied to G-protein coupled receptors on a cell membrane, we discriminated receptor oligomerization and clustering and revealed ligand-regulated receptor distribution patterns. This is the first example of adapting nanoparticle self-blinking for molecular counting, and this demonstrates the power of CDs as SMLM probes to reliably decipher sub-diffraction structures that mediate crucial biological functions.
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http://dx.doi.org/10.1021/acs.analchem.0c04885DOI Listing
March 2021

Protocol for a gallbladder cancer registry study in China: the Chinese Research Group of Gallbladder Cancer (CRGGC) study.

BMJ Open 2021 02 16;11(2):e038634. Epub 2021 Feb 16.

Department of HPB Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Introduction: Gallbladder cancer (GBC), the sixth most common gastrointestinal tract cancer, poses a significant disease burden in China. However, no national representative data are available on the clinical characteristics, treatment and prognosis of GBC in the Chinese population.

Methods And Analysis: The Chinese Research Group of Gallbladder Cancer (CRGGC) study is a multicentre retrospective registry cohort study. Clinically diagnosed patient with GBC will be identified from 1 January 2008 to December, 2019, by reviewing the electronic medical records from 76 tertiary and secondary hospitals across 28 provinces in China. Patients with pathological and radiological diagnoses of malignancy, including cancer in situ, from the gallbladder and cystic duct are eligible, according to the National Comprehensive Cancer Network 2019 guidelines. Patients will be excluded if GBC is the secondary diagnosis in the discharge summary. The demographic characteristics, medical history, physical examination results, surgery information, pathological data, laboratory examination results and radiology reports will be collected in a standardised case report form. By May 2021, approximately 6000 patient with GBC will be included. The clinical follow-up data will be updated until 5 years after the last admission for GBC of each patient. The study aimed (1) to depict the clinical characteristics, including demographics, pathology, treatment and prognosis of patient with GBC in China; (2) to evaluate the adherence to clinical guidelines of GBC and (3) to improve clinical practice for diagnosing and treating GBC and provide references for policy-makers.

Ethics And Dissemination: The protocol of the CRGGC has been approved by the Committee for Ethics of Xinhua Hospital, Shanghai Jiao Tong University School of Medicine (SHEC-C-2019-085). All results of this study will be published in peer-reviewed journals and presented at relevant conferences.

Trial Registration Number: NCT04140552, Pre-results.
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http://dx.doi.org/10.1136/bmjopen-2020-038634DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888310PMC
February 2021

Injectable thermosensitive hydrogel-based drug delivery system for local cancer therapy.

Colloids Surf B Biointerfaces 2021 Apr 16;200:111581. Epub 2021 Jan 16.

Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, 570100, China. Electronic address:

Injectable thermosensitive hydrogel has been regarded as attractive drug delivery system, which displays a sol-gel phase transition upon injection in response to temperature. Recently, thermosensitive hydrogel has become a matter of importance in cancer therapy, providing high local drug concentration, sustained release characteristics, minimal invasiveness, and low systemic toxicities. Here, we review the extensive application of thermosensitive hydrogel in local cancer therapy, including chemotherapy, photothermal therapy, photodynamic therapy, gene therapy, chemo-photothermal combined therapy, and chemo-/immuno- combined therapy.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111581DOI Listing
April 2021

Fried-food consumption and risk of cardiovascular disease and all-cause mortality: a meta-analysis of observational studies.

Heart 2021 Jan 19. Epub 2021 Jan 19.

Department of Biostatistics and Epidemiology, Shenzhen University Health Science Center, Shenzhen, Guangdong, China

Objective: We performed a meta-analysis, including dose-response analysis, to quantitatively determine the association of fried-food consumption and risk of cardiovascular disease and all-cause mortality in the general adult population.

Methods: We searched PubMed, EMBASE and Web of Science for all articles before 11 April 2020. Random-effects models were used to estimate the summary relative risks (RRs) and 95% CIs.

Results: In comparing the highest with lowest fried-food intake, summary RRs (95% CIs) were 1.28 (1.15 to 1.43; n=17, I=82.0%) for major cardiovascular events (prospective: 1.24 (1.12 to 1.38), n=13, I=75.7%; case-control: 1.91 (1.15 to 3.17), n=4, I=92.1%); 1.22 (1.07 to 1.40; n=11, I=77.9%) for coronary heart disease (prospective: 1.16 (1.05 to 1.29), n=8, I=44.6%; case-control: 1.91 (1.05 to 3.47), n=3, I=93.9%); 1.37 (0.97 to 1.94; n=4, I=80.7%) for stroke (cohort: 1.21 (0.87 to 1.69), n=3, I=77.3%; case-control: 2.01 (1.27 to 3.19), n=1); 1.37 (1.07 to 1.75; n=4, I=80.0%) for heart failure; 1.02 (0.93 to 1.14; n=3, I=27.3%) for cardiovascular mortality; and 1.03 (95% CI 0.96 to 1.12; n=6, I=38.0%) for all-cause mortality. The association was linear for major cardiovascular events, coronary heart disease and heart failure.

Conclusions: Fried-food consumption may increase the risk of cardiovascular disease and presents a linear dose-response relation. However, the high heterogeneity and potential recall and misclassification biases for fried-food consumption from the original studies should be considered.
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http://dx.doi.org/10.1136/heartjnl-2020-317883DOI Listing
January 2021

microRNA-377 acts as a suppressor in esophageal squamous cell carcinoma through CBX3-dependent P53/P21 pathway.

J Cell Physiol 2021 Jan 16;236(1):107-120. Epub 2020 Aug 16.

Department of Thoracic Surgery Oncology, Jiangxi Cancer Hospital, Nanchang, China.

Stem cells play pivotal roles in esophageal squamous cell carcinoma (ESCC) recurrence and metastasis. The self-renewal ability of stem cells was associated with specific microRNAs (miRs). Herein, we identified the effects of miR-377 on ESCC stem cell activities. First, the expression of miR-377 in ESCC and adjacent normal tissues was determined. The relationship between miR-377 and chromobox protein homolog 3 (CBX3) was assessed by a dual-luciferase reporter gene assay. miR-377 was overexpressed or inhibited in ESCC stem cells to explore its role in ESCC. To further investigate the mechanism of miR-377 in ESCC, cells were introduced with short hairpin RNA against CBX3 or pifithrin-α (inhibitor of P53 pathway). Besides, the expression of P21, P53, CD133, CD13, Nanog, sex determining region Y-Box 2 (Sox2), and octamer-binding transcription factor 4 (Oct4), cell sphere formation, colony formation, and proliferation were evaluated respectively. Finally, limiting dilution assay in vivo and tumor xenograft in nude mice were conducted to confirm the roles of miR-377 in vivo. miR-377 was poorly expressed in ESCC. Overexpression of miR-377 could suppress the stem-like trait of ESCC as well as the tumor growth in vivo. miR-377 targeted CBX3 to activate the P53/P21 pathway. Besides, the expression of stem-like markers including CD133, CD13, Oct4, Sox2, and Nanog was decreased, and the abilities of cell sphere formation, colony formation, proliferation, and tumorigenicity were significantly reduced by overexpressing miR-377 or silencing CBX3. The results were reversed after inactivating the P53/P21 pathway. In summary, upregulation of miR-377 inhibits the self-renewal of ESCC stem cells by inhibiting CBX3 expression and promoting activation of the P53/P21 pathway.
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http://dx.doi.org/10.1002/jcp.29631DOI Listing
January 2021

Determining the stability of minimally displaced lateral humeral condyle fractures in children: ultrasound is better than arthrography.

J Orthop Surg Res 2021 Jan 9;16(1):32. Epub 2021 Jan 9.

Department of Pediatric Orthopedic Surgery, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, 100 Hong-Kong road, Wuhan, 430016, People's Republic of China.

Background: Evaluating of the articular cartilage status of the distal humeral epiphysis is difficult. Ultrasound imaging of the elbow is increasingly being used to confirm the integrity of the articular cartilage of minimally displaced lateral humeral condyle fractures in children with minimally displaced fractures. The aims of this study were to assess the correlations between ultrasound and arthrography findings for predicting the integrity of the cartilage hinge and to describe the utility of ultrasound in determining the need for pre-treatment.

Methods: Thirty-nine patients with minimally displaced lateral humeral condyle fractures who underwent ultrasound and arthrography examinations before surgery from May 2018 to December 2019 were included in this study. Ultrasound and arthrography predictors of the cartilage hinge status were independently measured. The ultrasound and arthrography results were compared.

Results: The mean displacement of the fractures was 3.1 mm (range, 2.0~5.0 mm). Arthrography showed incomplete fractures in 24 patients (61.5%) and complete fractures in 15 patients (38.5%). Ultrasound showed incomplete fractures in 25 patients (64.1%) and complete fractures in 14 patients (35.9%). The ultrasound and arthrography results of the integrity of the articular surface were consistent in 92.3% of the cases, including 23 that were predicted to have an intact articular surface and 13 that were predicted to have an incongruity articular surface. There was no correlation between the displacement and the fracture appearing complete on the ultrasound scan. The Pearson coefficient between ultrasound and arthrography for assessing the integrity of the articular surface was 0.837.

Conclusions: Ultrasound and arthrography assessments of the integrity of the cartilage hinge status appear to be highly consistent. Ultrasound can be used as a complementary tool with arthrography to predict the integrity of the cartilage hinge status in children with minimally displaced lateral humeral condyle fractures.

Level Of Evidence: Prospective study; level II.
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http://dx.doi.org/10.1186/s13018-020-02174-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797123PMC
January 2021

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen.

Analyst 2021 Feb;146(4):1340-1345

College of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan, 030006, China.

Prostate specific antigen (PSA) has been considered as the most potential serological biomarker for the early stage detection of prostate cancer. Here, a label-free fluorescence aptasensing strategy for detecting PSA based on hybridization chain reaction (HCR) and G-quadruplex DNAzymes has been developed. This designed strategy consists of three DNA probes, aptamer probe (AP), hairpin probe 1 (H1) and hairpin probe 2 (H2). In the presence of target PSA, the aptamer sequences in AP specifically recognized PSA to form a PSA-aptamer complex, causing an AP conformation change and thus releasing the initiator, which triggered the chain-like assembly of H1 and H2 that yielded extended nicked double-stranded DNA through HCR. Upon the addition of hemin, the G-rich segments at the end of H1 and H2 self-assembled into the peroxidase-mimicking hemin/G-quadruplex DNAzymes, which catalyzed the hydrogen peroxide-mediated oxidation of thiamine to give a fluorescence signal dependent on the concentration of PSA. Under optimal conditions, a limit of detection of 0.05 nM and a linear range from 0.1 nM to 1 nM (R2 = 0.9942) were achieved by this assay. In addition, other interfering proteins, such as IgG, AFP and CEA, did not produce any significant change in the fluorescence intensity response, indicating good selectivity of this sensor for PSA detection. Finally, this proposed aptasensor was successfully used for diluted serum samples.
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http://dx.doi.org/10.1039/d0an02188aDOI Listing
February 2021

An Electrically Actuated Soft Artificial Muscle Based on a High-Performance Flexible Electrothermal Film and Liquid-Crystal Elastomer.

ACS Appl Mater Interfaces 2020 Dec 7;12(50):56338-56349. Epub 2020 Dec 7.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, No.28, Xianning West Road, Xi'an, Shaanxi 710049, P.R. China.

Liquid-crystal elastomer (LCE)-based soft robots and devices via an electrothermal effect under a low driving voltage have attracted a great deal of attention for their ability on generating larger stress, reversible deformation, and versatile actuation modes. However, electrothermal materials integrated with LCE easily induce the uncertainty of a soft actuator due to the non-uniformity on temperature distribution, inconstant resistance in the deformation process, and slow responsivity after voltage on/off. In this paper, a low-voltage-actuated soft artificial muscle based on LCE and a flexible electrothermal film is presented. At 6.5 V, a saturation temperature of 189 °C can be reached with a heating rate of 21 °C/s, which allows the soft artificial muscle quick and significant contraction and is suitable for untethered operation. Meanwhile, uniform temperature distribution and stable resistance of the flexible electrothermal film in the deformation process are obtained, leading to a work density of 9.97 kJ/m, an actuating stress of 0.46 MPa, and controllable deformation of the soft artificial muscle. Finally, programmable low-voltage-controlled soft artificial muscles are fabricated by tailoring the flexible electrothermal film or designing structured heating pattern, including a prototype of soft finger-like gripper for transporting small objects, which clearly demonstrates the potential of low-voltage-actuated soft artificial muscles in soft robotics applications.
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http://dx.doi.org/10.1021/acsami.0c17327DOI Listing
December 2020

Added value of hybrid SPECT with CT imaging for predicting poor therapeutic efficacy of Sr in patients with bone metastasis.

Sci Rep 2020 12 3;10(1):21207. Epub 2020 Dec 3.

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 400030, China.

To utilize single-photon emission computed tomography/computed tomography (SPECT/CT) scanning to investigate the usefulness of nerve root compression (NRC) and radioactive cold zone lesions (RCZLs) for predicting poor therapeutic efficacy of strontium-89 chloride (Sr-89) in patients with bone metastasis. Patients with bone metastatic neoplasms who had undergone baseline bone SPECT/CT scanning before Sr-89 therapy (148 MBq Sr-89 chloride by an intravenous injection for each patient) between July 2011 and July 2018 were included. Bone SPECT/CT images were assessed by two readers independently. Associations between imaging features and therapeutic efficacy were obtained via multivariate logistic regression analysis. Of 231 patients analyzed, 50 (21.6%) had NRC at baseline. Of 31 patients who experienced poor therapeutic efficacy, 29 (93.5%) had NRC. In multivariate logistic regression analysis baseline NRC independently predicted poor therapeutic efficacy. The sensitivity of NRC for predicting poor therapeutic efficacy was 93.5%, specificity was 89.5%, positive predictive value was 58.0%, and negative predictive value was 98.9%. RCZLs were detected in17 patients (7.4%), of whom 14 experienced poor Sr-89 therapeutic efficacy. The sensitivity of the presence of RCZLs for predicting poor therapeutic efficacy was 45.2%, specificity was 98.5%, positive predictive value was 82.4%, and negative predictive value was 92.1%. After adjusting for age, bone metabolism and lesion type, the significant independent predictors of poor Sr-89 therapeutic efficacy were presence of NRC (p < 0.001) and RCZL (p = 0.001). NRC and RCZL on baseline bone SPECT/CT are reliable independent predictors of poor Sr-89 therapeutic efficacy in patients with bone metastasis. These associations may facilitate the administration of more effective therapeutic interventions.
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http://dx.doi.org/10.1038/s41598-020-78372-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7713234PMC
December 2020

Antifade Carbon Dots on a Plasmonic Substrate for Enhanced Protein Detection in Immunotherapy.

ACS Sens 2020 12 30;5(12):4027-4034. Epub 2020 Nov 30.

State Key Laboratory of Heavy Oil Processing and College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.

Fluorescence microscopic analysis of checkpoint protein expression is capable of predicting clinical outcomes for checkpoint blockade immunotherapy. However, accurate detection of their expression levels is hindered by fluorophore photobleaching and cell autofluorescence. We now develop a sensitive and robust fluorescence microscopy method that uses antifade graphite-structured carbon dots (GCDs) on a plasmonic Ag substrate (named ACPAS) for the accurate detection of checkpoint proteins in immunotherapy. In ACPAS, a Ag substrate is used to enhance the fluorescence of GCDs while a continuous illumination is implemented to quench cell autofluorescence, thus enabling a dramatic improvement in the signal-to-background ratio by up to 33-fold. We use ACPAS to monitor programmed death ligand-1 (PD-L1) expression levels on various tumor cells and finely differentiate their microscopic changes in combination with chemokine receptor CXCR4-targeted treatments. ACPAS analysis reveals for the first time that CXCR4 agonist (SDF-1α) and antagonist (AMD3100) can potentiate PD-L1 expression by down-regulating CXCR4 expression on tumor cells, which provides valuable information on the development of anti-PD-L1 and anti-CXCR4 combination therapy. We envision that ACPAS will become a broadly useful tool for protein expression studies in biomedicine and life sciences.
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http://dx.doi.org/10.1021/acssensors.0c01983DOI Listing
December 2020

Notes from the Field: Effectiveness of Prevention and Control Measures for Imported COVID-19 in Guangming District of Shenzhen.

Eval Health Prof 2021 03 18;44(1):93-97. Epub 2020 Nov 18.

Department of Biostatistics and Epidemiology, School of Public Health, 47890Shenzhen University Health Science Center, Shenzhen, Guangdong, People's Republic of China.

Novel coronavirus disease 2019 (COVID-19) was present in most provinces of China after January 2020. We implemented a surveillance and screening strategy that included early detection of laboratory-confirmed COVID-19 cases and people who were exposed to the disease in Guangming District of Shenzhen. Separate targeted treatment and management strategies were applied to confirmed and suspected cases. From January 23 to March 13, 2020, we found 12 suspected cases, and 11 were confirmed as positive. Although eight of the 11 confirmed cases were family-aggregated, they were all imported cases with common exposure, which did not further cause local community transmission, and no medical staff were infected. After February 14, when the last case was confirmed, there were no newly confirmed cases for 28 consecutive days under the strict outbreak control. The targeted and whole-society involved prevention and control measures prevented the spread of the disease in a very short time and provided a strong guarantee for the orderly recovery of returning to work and social activities.
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http://dx.doi.org/10.1177/0163278720971833DOI Listing
March 2021

A novel aptasensor based on HCR and G-quadruplex DNAzyme for fluorescence detection of Carcinoembryonic Antigen.

Talanta 2021 Jan 28;221:121451. Epub 2020 Jul 28.

College of Chemistry and Environmental Engineering, Shanxi Datong University, Datong, 037009, China; College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China. Electronic address:

In this paper, a rationally designed aptasensing platform based on Hybridization Chain Reaction (HCR) and G-quadruplex DNAzyme for the fluorescence detection of Carcinoembryonic Antigen (CEA) has been developed. In the presence of target CEA, the aptamer sequence in Aptamer Probe (AP) specifically bound to CEA, resulting in the AP conformation change and thus releasing initiator, which triggered the autonomous cross-opening of Hairpin 1 (H1) and Hairpin 2 (H2) that yielded extended nicked double-stranded DNA via HCR. Upon the addition of hemin, G-rich segments at the end of H1 and H2 self-assembled into the peroxidase-mimicking hemin/G-quadruplex DNAzymes, which catalyzed the hydrogen peroxide-mediated oxidation of thiamine to achieve fluorescence detection of CEA. The HCR product, and the formation and catalytic performance of DNAzyme were characterized by agarose gel electrophoresis, UV-vis spectroscopy and fluorescence spectroscopy, respectively. Under optimal conditions, the fluorescent aptasensor showed a linear relationship ranging from 0.25 to 1.5 nM toward CEA with a detection limit of 0.2 nM. In addition, this aptasensor exhibited high selectivity for CEA without being affected by other interfering proteins, such as IgG, AFP and PSA. Furthermore, this proposed aptasensor was successfully applied to CEA analysis in diluted human serum samples. It is believed that this strategy has a promising potential in biochemical analysis and clinic application.
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http://dx.doi.org/10.1016/j.talanta.2020.121451DOI Listing
January 2021

Long Non-Coding RNA Regulates Expression to Accelerate Tumor Progression in Non-Small Cell Lung Cancer by Sponging .

Cancer Manag Res 2020 25;12:9113-9123. Epub 2020 Sep 25.

Department of Thoracic Surgery, Gansu Gem Flower Hospital, Lanzhou 730060, Gansu, People's Republic of China.

Background: Non-small cell lung cancer (NSCLC) is the most common type of lung carcinoma. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 () was identified to participate in tumor progression. However, the mechanism and functions of were rarely reported in NSCLC progression.

Methods: The relative gene expression was tested by qRT-PCR. Cell viability, apoptosis, migration and invasion were measured by MTT assay, flow cytometry, and transwell migration and invasion assays, respectively. The interactions between and or were predicted by starBase and confirmed by the dual-luciferase reporter assay and RNA pull-down assay. The protein level of was evaluated by Western blot assay.

Results: The levels of and were significantly increased, while the level of was apparently reduced in NSCLC tissues and cells. was verified to sponge and was identified as a direct target of . depletion repressed cell viability, migration and invasion, but induced the apoptotic rate by targeting . overexpression blocked cell viability, metastasis and promoted cell apoptosis by regulating . silencing down-regulated expression by sponging .

Conclusion: facilitated NSCLC progression by regulating expression via targeting .
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http://dx.doi.org/10.2147/CMAR.S250893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524175PMC
September 2020

Variation of dissolved organic matter during excess sludge reduction in microbubble ozonation system.

Environ Sci Pollut Res Int 2021 Feb 28;28(5):6090-6098. Epub 2020 Sep 28.

Shanghai Engineering Research Center of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

Sewage sludge is the major by-product of wastewater treatment plants, and about 30% readily biodegradable organic matters might be reused through the mass reduction process, which could be also reduced the disposal fee. In this study, the microbubble ozonation (MB-O) was employed to improve the oxidation efficiency for sludge solubilization. At 160 mgO/gSS, the maximum mixed liquor suspended solids (MLSS) reduction ratio was 37.5% and the protein and polysaccharide contents increased to 31.6 and 138.6 mg/L, respectively. It was proposed that aromatic protein and soluble microbial in sludge were oxidized preferentially by MB-O, and the dissolved organic matter (DOM) fractions (mainly humic-acid-like substances) exhibited low degradability according to the variations of fluorescence excitation-emission spectrum coupled with fluorescence regional integration. MB-O could enhance the settleability, but deteriorate sludge dewaterability at low dosage (< 160 mgO/gSS) due to a reduction in particle size from 61.7 to 47.5 μm. MB-O has a good performance on the mass reduction of sludge through the improvement of the radical generated.
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http://dx.doi.org/10.1007/s11356-020-10799-9DOI Listing
February 2021

Association of ABCG1 gene methylation and its dynamic change status with incident type 2 diabetes mellitus: the Rural Chinese Cohort Study.

J Hum Genet 2021 Apr 23;66(4):347-357. Epub 2020 Sep 23.

Department of Biostatistics and Epidemiology, School of Public Health, Shenzhen Univerity Health Science Center, Shenzhen, PR China.

To explore whether DNA methylation of the ATP-binding cassette G1 (ABCG1) gene and its dynamic change are associated with incident type 2 diabetes mellitus (T2DM). We conducted a nested case-control study with 286 pairs of T2DM cases and matched controls nested in the Rural Chinese Cohort Study. Conditional logistic regression models were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for incident T2DM risk according to ABCG1 methylation level at baseline and its dynamic change at follow-up examination. Spearman's rank correlation coefficients were used to analyze the association between ABCG1 methylation and its possible risk factors in the control group. We found that T2DM risk increased by 16% (OR = 1.16, 95% CI = 1.02-1.31) with each 1% increase in DNA methylation levels of the ABCG1 loci CpG13 and CpG14. DNA methylation change of the ABCG1 locus CpG15 during the 6-year follow-up was associated with increased T2DM risk: T2DM risk increased by 78% in the upper tertile group (methylation gain ≥5%) versus lower tertile group (methylation gain <1%) (OR = 1.78, 95% CI = 1.01-3.15). Furthermore, body mass index was positively correlated with the DNA methylation level of the ABCG1 loci CpG13, CpG14 and CpG15. In conclusion, DNA methylation levels of the ABCG1 loci CpG13 and CpG14 and the methylation gain of locus CpG15 were positively associated with incident T2DM risk, which may suggest a possible etiologic pattern for T2DM and potentially improve T2DM prediction in rural Chinese people.
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http://dx.doi.org/10.1038/s10038-020-00848-zDOI Listing
April 2021
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