Publications by authors named "Xiaoli Zhou"

114 Publications

Study on the structure and digestibility of high amylose Tartary buckwheat (Fagopyrum tataricum Gaertn.) starch-flavonoid prepared by different methods.

J Food Sci 2021 Apr 5;86(4):1463-1474. Epub 2021 Apr 5.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, China.

Tartary buckwheat (Fagopyrum tataricum Gaertn.) is the only food rich in flavonoid bioactive substances in grains. Studies have shown that flavonoids interaction with amylose has an important impact on the physical and chemical properties and structure of starch. In this study, Tartary buckwheat was used as a raw material. It was then threshed with pullulanase, and a high amylose Tartary buckwheat starch flavonoid complex (HBS/BF) was prepared by physical mixing (PM), water bath treatment (WT), acid-base precipitation (AP), microwave treatment (MT), and ultrasonic treatment (UT); the physical and chemical properties were then evaluated. The results show that HBS/BF-UT and HBS/BF-MT have a higher iodine binding rate than HBS/BF-PM; X-ray diffraction results show that HBS/BF-AP has a V-type crystal form, but the relative crystallinity was reduced. Fourier infrared spectroscopy showed that there is no new covalent bond between Tartary buckwheat starch and flavonoids. In vitro digestion showed that adding flavonoid significantly increased the digestibility of Tartary buckwheat starch. PRACTICAL APPLICATION: These results will provide a theoretical basis for further starch anti-digestion mechanisms and the preparation of resistant starch. These steps will provide insights into the application of Tartary buckwheat starch and flavonoids in the food industry.
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http://dx.doi.org/10.1111/1750-3841.15657DOI Listing
April 2021

Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months.

Virol Sin 2021 Mar 4. Epub 2021 Mar 4.

National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection, the protection period of vaccination and the possibility of building an active herd immunity. This retrospective cohort study included 172 COVID-19 patients who were hospitalized in Wuhan. A total of 404 serum samples were obtained over six months from hospitalization to convalescence. Antibodies in the specimens were quantitatively analyzed by the capture chemiluminescence immunoassays (CLIA). All patients were positive for the anti-SARS-CoV-2 IgM/IgG at the onset of COVID-19 symptoms, and the IgG antibody persisted in all the patients during the convalescence. However, only approximately 25% of patients can detect the IgM antibodies, IgM against N protein (N-IgM) and receptor binding domain of S protein (RBD-IgM) at the 27th week. The titers of IgM, N-IgM and RBD-IgM reduced to 16.7%, 17.6% and 15.2% of their peak values respectively. In contrast, the titers of IgG, N-IgG and RBD-IgG peaked at 4-5th week and reduced to 85.9%, 62.6% and 87.2% of their peak values respectively at the end of observation. Dynamic behavior of antibodies and their correlation in age, gender and severity groups were investigated. In general, the COVID-19 antibody was sustained at high levels for over six months in most of the convalescent patients. Only a few patients with antibody reducing to an undetectable level which needs further attention. The humoral immune response against SARS-CoV-2 infection in COVID-19 patients exhibits a typical dynamic of acquired immunity.
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http://dx.doi.org/10.1007/s12250-021-00360-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931792PMC
March 2021

Serum hsa_circ_0054633 Is Elevated and Correlated with Clinical Features in Type 2 Diabetes Mellitus.

Ann Clin Lab Sci 2021 Jan;51(1):90-96

Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, Ningxia Medical University General Hospital, Yinchuan, Ningxia, China

Objective: Type 2 diabetes mellitus (T2DM) is a chronic disease that seriously threatens human health with high incidence and various complications. Circular RNA has become a research hotspot in recent years due to its high stability and wide expression, and has been found to be related to many diseases. The main purpose of this study was to investigate the expression of serum hsa_circ_0054633 in T2DM patients and explore the potential role of serum hsa_circ_0054633 in T2DM diagnosis and its association with clinical characteristics.

Methods: This retrospective case-control study enrolled 88 participants including 44 patients with T2DM and 44 age- and sex-matched controls between January 2018 and March 2019 at Ningxia Medical University General Hospital. Serum hsa_circ_0054633 levels were measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) with absolute quantification. Baseline information including clinical background, glucose control and biochemical variables were collected. Receiver operating characteristic (ROC) curves were used to evaluate diagnostic effect.

Results: We found that serum expression of hsa_circ_0054633 was dramatically increased in patients with T2DM. Serum hsa_circ_0054633 levels had high sensitivity (75.0%) and specificity (95.5%) for differentiating T2DM. Besides, serum expression of circ_0054633 was significantly correlated with fasting blood glucose (=0.698), hemoglobin A1c (=0.503) and low-density lipoprotein (=0.399).

Conclusion: Serum hsa_circ_0054633 is a potential noninvasive biomarker in discerning T2DM and is associated with changes in blood glucose and lipid.
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January 2021

Source and spatial distribution of airborne heavy metal deposition studied using mosses as biomonitors in Yancheng, China.

Environ Sci Pollut Res Int 2021 Feb 16. Epub 2021 Feb 16.

Co-Innovation Centre for Sustainable Forestry in Southern China, College of Biology and the Environment, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, Nanjing Forestry University, Nanjing, 210037, China.

Naturally growing mosses have been successfully used as biomonitors of atmospheric heavy metal (HM) deposition. In recent years, with rapid economic development, environmental pollution in Yancheng, a coastal city in central Jiangsu Province, China, has become increasingly serious. However, to date, there have been no reports on atmospheric HM deposition in Yancheng. In this study, we investigated the HM concentrations and Pb isotopes in the moss Haplocladium microphyllum (Hedw.) Broth. from Yancheng and analyzed their main sources. The concentrations of HM in mosses from Yancheng were higher than those recorded in other studies of mosses from HM smelting regions and pollution-free areas of Eurasia and Alaska. The contamination factor value suggested that the pollution level of Cd was the highest. The pollution load index indicated that the studied area was severely contaminated with Cd, Cr, Pb, Zn, V, Ni, and Cu. Positive matrix factorization was employed to identify the contamination sources of HM and apportion their source contributions in mosses. The contributions of the natural source, together with manufacturing and construction, metal processing and chemical industries, traffic emissions and fuel burning in industrial activities, and agricultural activities, accounted for 53%, 33%, 12%, and 2%, respectively. The Pb isotopic ratios in the mosses (1.125-1.164 for Pb/Pb, 2.059-2.148 for Pb/Pb) further proved that metal processing and traffic emissions were the main sources of Pb contamination. These results are useful for developing various effective measures to prevent and reduce atmospheric HM deposition in Yancheng.
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http://dx.doi.org/10.1007/s11356-021-12814-zDOI Listing
February 2021

Clinical characteristics in patients with coronary slow flow phenomenon: A retrospective study.

Medicine (Baltimore) 2021 Feb;100(6):e24643

Department of Cardiology, the First Affiliated Hospital of Chongqing Medical University.

Abstract: Coronary slow flow phenomenon (CSFP) is a coronary artery disease in which coronary angiography shows no obvious stenosis, but there is a delay in blood flow perfusion. The etiopathogenic mechanisms of CSFP are still unclear. The aim of the present study was to investigate the role of clinical characteristics in patients with CSFP, and to provide a reference for exploring the potential mechanisms of CSFP. Patients with angiographically normal epicardial arteries were enrolled (145 patients with CSFP and 145 normal controls). Collected clinical information and laboratory indexes, which measured by peripheral venous blood samples before coronary angiography. Logistic regression analysis was performed for statistical analysis. The present study found 19 clinical and laboratory indexes with statistical differences between the two groups in univariate analysis. Multivariate analysis showed that monocyte count, haemoglobin, serum creatinine and globulin were independent predictors of CSFP. Moreover, the monocyte count, haemoglobin, creatinine and globulin levels were significantly higher in the CSFP patients than the controls, with positive associations between these parameters and the extent of CSFP. In addition, ROC analysis showed the diagnostic value of the above indexes for CSFP.
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http://dx.doi.org/10.1097/MD.0000000000024643DOI Listing
February 2021

Effects of FGFR4 G388R, V10I polymorphisms on the likelihood of cancer.

Sci Rep 2021 Jan 14;11(1):1373. Epub 2021 Jan 14.

Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi, 214000, People's Republic of China.

The correlation between G388R or V10I polymorphisms of fibroblast growth factor receptor (FGFR) 4 gene and the risk of carcinoma has been investigated previously, but the results are contradictory. Odds ratios (ORs) with 95% confidence intervals (95%CIs), in silico tools, and immunohistochemical staining (IHS) were adopted to assess the association. In total, 13,793 cancer patients and 16,179 controls were evaluated in our pooled analysis. Summarization of all the studies showed that G388R polymorphism is associated with elevated susceptibility to cancer under homozygous comparison (OR = 1.21, 95%CI = 1.03-1.43, P = 0.020) and a recessive genetic model (OR = 1.21, 95%CI = 1.04-1.41, P = 0.012). In the stratification analysis by cancer type and ethnicity, similar findings were indicated for prostate cancer, breast cancer, and individuals of Asian descendant. Polyphen2 bioinformatics analysis showed that the G388R mutation is predicted to damage the protein function of FGFR4. IHS analysis indicated that FGFR4 expression is increased in advanced prostate cancer. These findings may guide personalized treatment of certain types of cancers. Up-regulation of FGFR4 may be related to a poor prognosis in prostate cancer.
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http://dx.doi.org/10.1038/s41598-020-80146-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809464PMC
January 2021

A myxobacterial LPMO10 has oxidizing cellulose activity for promoting biomass enzymatic saccharification of agricultural crop straws.

Bioresour Technol 2020 Dec 16;318:124217. Epub 2020 Oct 16.

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China. Electronic address:

Myxobacteria are soil microorganisms with the ability to break down biological macromolecules due to the secretion of a large number of extracellular enzymes, but there has been no research report on myxobacterial lytic polysaccharide monooxygenases (LPMOs). In this study, two LPMO10s, ViLPMO10A and ViLPMO10B, from myxobacterium Vitiosangium sp. GDMCC 1.1324 were characterized. Of which, ViLPMO10B is a C1-oxidizing cellulose-active LPMO. Moreover, ViLPMO10B could decrease the degree of polymerization of crop straws cellulose and synergize with commercial cellulase to promote the saccharification. When the weight ratio of commercial cellulase to ViLPMO10B was 9:1, the conversion efficiency of corn stalk, sugarcane bagasse, and rice straw into reducing sugar was improved by 17%, 16%, and 22%, respectively, compared with commercial cellulase without ViLPMO10B. These results indicate that ViLPMO10B has the potential to be a component of a high-efficient cellulase cocktail and has application value in the saccharification of agricultural residual biomasses.
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http://dx.doi.org/10.1016/j.biortech.2020.124217DOI Listing
December 2020

Selection of homemade mask materials for preventing transmission of COVID-19: A laboratory study.

PLoS One 2020 15;15(10):e0240285. Epub 2020 Oct 15.

Center for Medical Device Supervision and Evaluation, Chinese Evidence-Based Medicine Center, West China Hospital, Sichuan University, Chengdu, China.

The Coronavirus Disease 2019 (COVID-19) has swept the whole world with high mortality. Since droplet transmission is the main route of transmission, wearing a mask serves as a crucial preventive measure. However, the virus has spread quite quickly, causing severe mask shortage. Finding alternative materials for homemade masks while ensuring the significant performance indicators will help alleviate the shortage of masks. Referring to the national standard for the "Surgical Mask" of China, 17 materials to be selected for homemade masks were tested in four key indicators: pressure difference, particle filtration efficiency, bacterial filtration efficiency and resistance to surface wetting. Eleven single-layer materials met the standard of pressure difference (≤49 Pa), of which 3 met the standard of resistance to surface wetting (≥3), 1 met the standard of particle filtration efficiency (≥30%), but none met the standard of bacterial filtration efficiency (≥95%). Based on the testing results of single-layer materials, fifteen combinations of paired materials were tested. The results showed that three double-layer materials including double-layer medical non-woven fabric, medical non-woven fabric plus non-woven shopping bag, and medical non-woven fabric plus granular tea towel could meet all the standards of pressure difference, particle filtration efficiency, and resistance to surface wetting, and were close to the standard of the bacterial filtration efficiency. In conclusion, if resources are severely lacking and medical masks cannot be obtained, homemade masks using available materials, based on the results of this study, can minimize the chance of infection to the maximum extent.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0240285PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561133PMC
October 2020

Very low risk of lymph node metastasis in Epstein-Barr virus-associated early gastric carcinoma with lymphoid stroma.

BMC Gastroenterol 2020 Aug 17;20(1):273. Epub 2020 Aug 17.

Department of Pathology of the Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, China.

Background: Epstein-Barr virus-associated early gastric carcinoma with lymphoid stroma (EBV-GCLS) is a rare variant of early gastric carcinomas. Clinicopathological features of this variant remain obscure, especially in Chinese patients. Therefore, we collected EBV-GCLS cases and studied clinicopathology and prognosis.

Methods: By a retrospective review of 595 consecutive radical gastrectomies for early gastric carcinoma from 2006 to 2018, we identified 8 (1.3%, 8/595) EBV-GCLS cases. Clinicopathologic characteristics were compared between EBV-GCLSs and 109 conventional early gastric carcinomas, which were divided into intramucosal, SM1, and SM2 subgroups. The latter 2 subgroups were classified according to the submucosal invasion depth below or over 500 μm.

Results: All 8 EBV-GCLSs occurred in male patients and invaded deep submucosa (SM2) without lymph node metastasis (LNM), four (50%) of which had synchronous non-gastric malignant tumors (3 gastric gastrointestinal stromal tumors and 1 primary clear cell renal cell carcinoma), and four (50%) arose in the proximal stomach. Compared to conventional early gastric carcinomas, EBV-GCLS was significantly more frequent with SM2 invasion, poor differentiation, and synchronous non-gastric carcinoma tumor, but not with age, gender, macroscopic type, location, size, perineural invasion, lymphovascular invasion, and pathologic stage. In invasion-depth stratified comparisons in the SM2 subgroup, the frequency of LNM in EBV-GCLS was significantly lower than that in conventional early gastric carcinomas (p < 0.05) and the 5-year survival rate of patients with EBV-GCLS was better than that with conventional early gastric carcinomas in 3 subgroups (100% vs 91.5, 85.7, 83.9%, respectively), although the differences did not reach a statistically significant level due to the small sample size. Significant differences among 4 subgroups were found in tumor grade, lymphovascular invasion, LNM, pathological stage, and synchronous tumor, but not in age, gender, macroscopic type, tumor size, location, perineural invasion.

Conclusions: Even with poor differentiation and SM2 invasion, EBV-GCLS showed very low risk of LNM and may be a candidate for endoscopic therapy such as endoscopic submucosal dissection.
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http://dx.doi.org/10.1186/s12876-020-01422-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7433105PMC
August 2020

Genetic Polymorphisms of / in Chronic Obstructive Pulmonary Disease.

COPD 2020 10 5;17(5):595-600. Epub 2020 Aug 5.

Department of General Practice, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan, China.

Chronic obstructive pulmonary disease (COPD) is a high incidence in the elderly and significantly affects the quality of life. and play an important role in tobacco-related diseases and inflammatory reactions. Thus, we aim to investigate the association between / polymorphisms and the risk of COPD. In this study, a total of 821 subjects were recruited which include 313 COPD cases and 508 healthy controls. Seven SNPs of / were selected for genotyping. The odds ratios (ORs) and 95% confidence interval (95% CI) were calculated using logistic regression analysis to evaluate the association between COPD risk and / polymorphisms. Our study showed that A allele of rs9332220 in was associated with reducing COPD risk (OR = 0.64, 95% CI = 0.43-0.94,  = 0.021). And rs111853758 G allele carrier could significantly decrease 0.35-fold COPD risk compared with T allele carrier (OR = 0.65, 95% CI = 0.45-0.96,  = 0.027). Furthermore, sex-based stratification analysis showed that rs9332220 and rs111853758 polymorphisms were associated with the risk of COPD in males. This is the first study to investigate the association between and genetic polymorphisms and COPD risk, which may give a new perspective on the prevention and diagnosis of COPD.
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http://dx.doi.org/10.1080/15412555.2020.1780577DOI Listing
October 2020

The effect of CYP3A4 genetic variants on the susceptibility to chronic obstructive pulmonary disease in the Hainan Han population.

Genomics 2020 11 30;112(6):4399-4405. Epub 2020 Jul 30.

Department of General Practice, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan, China. Electronic address:

Purpose: Genetic polymorphisms act a crucial role in chronic obstructive pulmonary disease (COPD) progression. This study aimed to investigate the correlation between CYP3A4 variants and COPD risk.

Methods: We carried out a case-control study of 821 individuals (313 patients and 508 healthy subjects) to identify the correlation of CYP3A4 SNPs with COPD risk in the Hainan Han population. The association was evaluated by Odds ratios (OR) and 95% confidence intervals (CI).

Results: Our study showed that rs4646437 polymorphism was related to a significantly increased susceptibility to COPD (OR 1.45, 95% CI = 1.10-1.90, p = 0.008). Stratified analyses indicated that rs4646437 polymorphism was significantly related to an increased risk of COPD in males (OR 1.95, 95% CI = 1.19-3.20, p = 0.008). However, rs4646440 played a protective role in females (OR 0.54, 95% CI = 0.31-0.93, p = 0.024). Rs4646437 was found to significantly improve the risk of COPD in smokers (OR 1.67, 95% CI = 1.12-2.48, p = 0.011). While rs4646440 had a significantly lower susceptibility to COPD in non-smokers (OR 0.64, 95% CI = 0.45-0.90, p = 0.010). Haplotype analysis revealed that AT haplotype of CYP3A4 was found to increase the risk of COPD in non-smokers (OR 1.71, 95% CI = 1.04-2.82, p = 0.034).

Conclusion: Our result gives a new understanding of the association between CYP3A4 gene and COPD in the Hainan Han population.
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http://dx.doi.org/10.1016/j.ygeno.2020.07.043DOI Listing
November 2020

Regulation of tartary buckwheat-resistant starch on intestinal microflora in mice fed with high-fat diet.

Food Sci Nutr 2020 Jul 12;8(7):3243-3251. Epub 2020 May 12.

Department of School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai China.

Resistant starch (RS) is closely related to the composition of intestinal flora. Based on many studies on the physiological functions of probiotics and short-chain fatty acids (SCFAs), it is possible that RS can improve the intestinal health of the host. Therefore, we speculated that tartary buckwheat-resistant starch (TBRS) can also regulate the intestinal flora disorder caused by high-fat diet. We randomly divided 36 SPF C57BL/6J mice into low-fat diet, high-fat diet (HF-CS), high-fat diet supplemented with TBRS (HF-BRS), and high-fat diet supplemented with corn-resistant starch (HF-CRS). We analyzed the diversity and richness of gut microbiota based on PCR and Illumina high-throughput sequencing technology. In community abundance, the HF-BRS group was significantly higher than the other three groups ( < .05). TBRS improved the gut microbiota dysbiosis, including decreasing the -to- ratios (F/B) and contributing to the growth of and as well significantly inhibiting the growth of , , and . We also analyzed the production of SCFAs by GC-MS, and the concentration of total SCFAs increased in the HF-CS group. However, TBRS significantly increased the production of SCFAs, especially the propionate concentration compared with the HF-CRS group ( < .05). These results elucidated that TBRS has the potential to improve intestinal health by altering the structure of gut microbiota and increasing the production of SCFAs. Our findings have important implications for TBRS as functional food ingredient to manipulate intestinal microflora.
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http://dx.doi.org/10.1002/fsn3.1601DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382121PMC
July 2020

Psychological trajectories of Chinese women undergoing pregnancy termination for foetal abnormality: A descriptive qualitative study using expressive writing.

J Clin Nurs 2020 Oct 23;29(19-20):3667-3678. Epub 2020 Jul 23.

Women's Hospital School of Medicine, Zhejiang University, Hangzhou, China.

Aims And Objectives: To explore the psychological trajectories of women who have had a diagnosis of foetal abnormality from the time of receiving a definite diagnosis to one month after discharge.

Background: Foetal abnormalities are not uncommon worldwide. Such situations are devastating, and the termination of a pregnancy due to foetal abnormalities is a traumatic and stressful event for the mother.

Design: A descriptive qualitative approach using expressive writing.

Methods: The study included 20 women recruited through purposive sampling at a tertiary hospital in China. The participants were asked to write four 15-min essays related to their experiences with foetal abnormalities. Thematic analysis was used to generate themes. The COREQ checklist (see Supporting Information Appendix S1) was used.

Results: Four themes that reflected the women's psychological trajectories were identified: (a) traumatic response, (b) ruminant meditation, (c) positive coping and (d) post-traumatic growth stages. The psychological trajectories were dynamically shaped, and different responses were displayed in each stage.

Conclusions: Various responses were observed during each of the four psychological trajectories, which indicated that awareness regarding the psychological impacts associated with foetal abnormalities should be increased. The present findings suggest that healthcare services should be made accessible for women who have had a diagnosis of foetal abnormality to allow them to receive targeted nursing care at different stages and improve their psychological well-being.

Relevance To Clinical Practice: Midwives, nurses and other healthcare professionals should pay more attention to the mental health of women pregnant with foetuses diagnosed with abnormalities. Interventions designed based on women's worries and demands during various phases should be offered. In terms of the present research method, expressive writing possesses unique advantages that can be applied for relevant qualitative research.
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http://dx.doi.org/10.1111/jocn.15386DOI Listing
October 2020

Plastrum Testudinis Extracts Promote NSC Differentiation into Dopaminergic Neuron by Regulating the Interaction of TET1 and FoxA2.

Evid Based Complement Alternat Med 2020 20;2020:9104135. Epub 2020 Apr 20.

School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China.

In recent years, stem cells have gained much attention for the treatment of neurodegenerative diseases. However, inducing neural stem cell directionally differentiation is a difficult problem in the treatment of Parkinson's disease (PD) by stem cell therapy. Plastrum Testudinis (PT) can enhance the number of TH-positive neurons in the PD rat brain substantia nigra, but the underlying mechanism has not been clarified. Here, we aimed at further investigating the mechanism by which PT can promote NSC differentiation into dopaminergic neurons. A rat model of PD was used for detecting the effect of PT on the rat brain substantia nigra in vivo. The results showed the expressions of tyrosine hydroxylase (TH) and TET1 enzyme were increased after treatment with PT. Consequently, Plastrum Testudinis extracts (PTEs) were used for inducing NSC differentiation into dopaminergic neurons ex vivo. During differentiation of NSCs induced by PTE, TH expression was increased, with a concomitant increase in both TET1 and FoxA2. Next, we performed coimmunoprecipitation analysis to examine the interaction between TET1 protein and FoxA2 protein. Our results show that PTE can increase the binding rate of TET1 and FoxA2. Thus, our findings show that PTE can increase the efficiency of NSCs to directionally differentiate into dopaminergic neurons and provide experimental evidence for PT in the treatment of Parkinson's disease.
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http://dx.doi.org/10.1155/2020/9104135DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189310PMC
April 2020

The Histological, Effectoromic, and Transcriptomic Analyses of Reveal an Upregulation of Multiple Genes Suppressing Infection.

Int J Mol Sci 2020 May 1;21(9). Epub 2020 May 1.

State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.

Utilization of disease resistance components from wild potatoes is a promising and sustainable approach to control blight. Here, we combined avirulence () genes screen with RNA-seq analysis to discover the potential mechanism of resistance in Mexican wild potato species, . Histological characterization displayed that hyphal expansion was significantly restricted in epidermal cells and mesophyll cell death was predominant, indicating that a typical defense response was initiated in . Inoculation of with diverse isolates showed distinct resistance patterns, suggesting that has complex genetic resistance to most of the prevalent races of in northwestern China. Further analysis by gene screens and comparative transcriptomic profiling revealed the presence and upregulation of multiple plant genes corresponding to biotic stresses. Six alleles of , , , , , and were detected, and over 60% of the 112 detected NLR proteins were significantly induced in . On the contrary, despite the expression of the , , and alleles, fewer NLR proteins were expressed in susceptible . Thus, the enriched genes in make it an ideal genetic resource for the discovery and deployment of resistance genes for potato breeding.
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http://dx.doi.org/10.3390/ijms21093211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247345PMC
May 2020

Effects of expressive writing intervention for women's PTSD, depression, anxiety and stress related to pregnancy: A meta-analysis of randomized controlled trials.

Psychiatry Res 2020 06 14;288:112933. Epub 2020 Apr 14.

Department of Obstetrics and Gynecology, Women's Hospital School of Medicine, Zhejiang University, Hangzhou, China. Electronic address:

Expressive writing (EW) is a common psychological intervention that aims to improve the mental health of traumatized individuals. Pregnancy is considered an anxious or traumatic experience for some women, and any crisis in relation to pregnancy is closely associated with their psychological well-being. Post-traumatic stress disorder (PTSD), depression, anxiety and stress are the most prominent emotional and psychological responses that may occur during the process. However, the effects of EW in mediating women's PTSD, depression, anxiety and stress related to pregnancy remain uncertain, and no qualified meta-analysis has assessed such effects. The aim of this study was to assess the effectiveness of EW as a psychological intervention for women. Five databases, including PubMed, EMBASE, Cochrane, Web of Science and PsycINFO, were searched from inception to September 2019 for eligible studies. Finally, a total of 929 participants from 8 randomized controlled trial (RCT) studies were included. A pooled analysis demonstrated that EW was an efficient therapy for decreasing PTSD. However, the results showed that the EW intervention was not associated with the expected effects on anxiety and stress symptoms. The efficacy of EW for depression was inconclusive. More RCTs are necessary to verify the effectiveness of EW for depression. Studies concentrating on EW's effects on physical symptoms are necessary, and researchers should create an EW intervention group, neutral writing group and no writing group to examine the true effects of EW. Future research should examine whether longer, more frequent, and more targeted writing interventions would result in different conclusions.
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http://dx.doi.org/10.1016/j.psychres.2020.112933DOI Listing
June 2020

The correlation between CYP4F2 variants and chronic obstructive pulmonary disease risk in Hainan Han population.

Respir Res 2020 Apr 15;21(1):86. Epub 2020 Apr 15.

Department of General Practice, Hainan General Hospital, Haikou, 570102, Hainan, China.

Background: Chronic obstructive pulmonary disease (COPD) is a complex pulmonary disease. Cytochrome P450 family 4 subfamily F member 2 (CYP4F2) belongs to cytochrome P450 superfamily of enzymes responsible for metabolism, its single nucleotide polymorphisms (SNPs) were reported to be involved in metabolism in the development of many diseases. The study aimed to assess the relation between CYP4F2 SNPs and COPD risk in the Hainan Han population.

Method: We genotyped five SNPs in CYP4F2 in 313 cases and 508 controls by Agena MassARRAY assay. The association between CYP4F2 SNPs and COPD risk were assessed by χ test and genetic models. Besides, logistic regression analysis was introduced into the calculation for odds ratio (OR) and 95% confidence intervals (CIs).

Results: Allele model analysis indicated that rs3093203 A was significantly correlated with an increased risk of COPD. Also, rs3093193 G and rs3093110 G were associated with a reduced COPD risk. In the genetic models, we found that rs3093203 was related to an increased COPD risk, while rs3093193 and rs3093110 were related to a reduced risk of COPD. After gender stratification, rs3093203, rs3093193 and rs3093110 showed the association with COPD risk in males. With smoking stratification, rs3093144 was significantly associated with an increased risk of COPD in smokers. CYP4F2 SNPs were significantly associated with COPD risk.

Conclusions: Our findings illustrated potential associations between CYP4F2 polymorphisms and COPD risk. However, large-scale and well-designed studies are needed to determine conclusively the association between the CYP4F2 SNPs and COPD risk.
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http://dx.doi.org/10.1186/s12931-020-01348-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161254PMC
April 2020

Genome-wide CRISPR knockout screens identify ADAMTSL3 and PTEN genes as suppressors of HCC proliferation and metastasis, respectively.

J Cancer Res Clin Oncol 2020 Jun 7;146(6):1509-1521. Epub 2020 Apr 7.

Department of Clinical Laboratory, Sichuan Provincial People's Hospital Affiliate Wenjiang Hospital, Wanchun East Road, Wenjiang District, Chengdu, 611130, China.

Purpose: It is important for hepatocellular carcinoma (HCC) treatment that the targets related to its progression are identified. Clustered regularly interspaced short palindromic repeat (CRISPR)-associated nuclease 9 (Cas9)-based genetic screening is a powerful tool for identifying genes with loss-of-function mutations that are critical for tumour growth and metastasis.

Methods: We transduced the human SMMC7721 HCC cell line expressing Cas9 with a human genome-scale CRISPR-Cas9 knockout (GeCKO) lentiviral library A (hGeCKOa) of 65,383 single-guide RNAs (sgRNAs) targeting 19,050 human genes; we then subcutaneously transplanted the transduced cells into nude mice.

Results: The transduced cells were found to proliferate and metastasize faster than the untransduced cells. Through next-generation sequencing, the genes potentially related to HCC proliferation and metastasis were identified. The sgRNAs targeting the ADAMTSL3 and PTEN genes appeared twice on the list of genes related to HCC proliferation and metastasis, respectively. Analysis based on the data mining of Oncomine revealed that the ADAMTSL3 and PTEN genes were expressed at lower levels in HCC cells than they were in normal liver cells, indicating their tumour-suppressive roles. Downregulation of ADAMTSL3 and PTEN displayed poor overall survival (OS) and predicted poor relapse-free survival (RFS), further supporting their tumour-suppressive roles. Moreover, knocking out either the ADAMTSL3 or PTEN genes promoted either the proliferation or metastasis of HCC cells, respectively.

Conclusions: Using both in vitro and in vivo approaches, we described the profound role of the ADAMTSL3 and PTEN genes. This study indicates novel candidate targets for use in HCC treatment and therapy.
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http://dx.doi.org/10.1007/s00432-020-03207-9DOI Listing
June 2020

Influence of the CYP2J2 Gene Polymorphisms on Chronic Obstructive Pulmonary Disease Risk in the Chinese Han Population.

Arch Bronconeumol 2020 11 26;56(11):697-703. Epub 2020 Mar 26.

Department of General Practice, Hainan General Hospital, Haikou 570311, Hainan, China. Electronic address:

Introduction: Cytochrome P450 (CYP) 2J2 is a major enzyme that controls epoxyeicosatrienoic acids biosynthesis, which may play a role in chronic obstructive pulmonary disease (COPD) development. In this study, we aimed to assess the influence of CYP2J2 polymorphisms with COPD susceptibility.

Material And Methods: A case-control study enrolled 313 COPD cases and 508 controls was to investigate the association between CYP2J2 polymorphisms and COPD risk. Agena MassARRAY platform was used to genotype CYP2J2 polymorphisms. Odds ratios (OR) and 95% confidence intervals (CI) were calculated to evaluate the association between CYP2J2 polymorphisms and COPD risk.

Results: We observed rs11207535 (homozygote: OR=0.08, 95%CI=0.01-0.96, p=0.047; recessive: OR=0.08, 95%CI=0.01-0.94, p=0.044), rs10889159 (homozygote: OR=0.08, 95%CI=0.01-0.92, p=0.043; recessive: OR=0.08, 95%CI=0.01-0.90, p=0.040) and rs1155002 (heterozygote: OR=1.63, 95%CI=1.13-2.36, p=0.009; dominant: OR=1.64, 95%CI=1.15-2.35, p=0.006; additive: OR=1.45, 95%CI=1.09-1.92, p=0.011) were significantly associated with COPD risk. Allelic tests showed T allele of rs2280274 was related to a decreased risk of COPD and T allele of rs1155002 was associated with an increased COPD risk. Stratified analyses indicated the effects of CYP2J2 polymorphisms and COPD risk were dependent on gender and smoking status (p<0.05). Additionally, two haplotypes (ACT and ACC) significantly decreased COPD risk.

Conclusion: It suggested CYP2J2 polymorphisms were associated with COPD susceptibility in the Chinese Han population.
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http://dx.doi.org/10.1016/j.arbres.2019.11.026DOI Listing
November 2020

RNA-Seq analysis revealed the molecular mechanisms of photobiomodulation effect on human fibroblasts.

Photodermatol Photoimmunol Photomed 2020 Jul 12;36(4):299-307. Epub 2020 Apr 12.

Oral Biomedical Engineering Laboratory, Shanghai Stomatological Hospital, Fudan University, Shanghai, China.

Background: The photobiomodulation (PBM) effect has been applied to various clinical therapy for a long time. However, the mechanism related to the PBM effect in terms of wavelengths has been lack of in-depth study, except that ultraviolet radiation has attracted much attention due to its strong cell-killing effect.

Purpose: To clarify the principle behind PBM and the main mechanism of improvement.

Methods: To carry on this study, we created light equipment using three LED chips, which emit 390 nm ultraviolet radiation, 415 nm blue light and 660 nm red light, respectively. We choose human fibroblasts (HF) to be irradiated by three different wavelengths for PBM test. In this study, we used cell counting kit (CCK-8) test to show the cell proliferation roughly and reported on a systematic RNA sequencing (RNA-seq) analysis at transcriptional expression levels from HF, which accepted PBM of different wavelengths of light.

Results: We found that 415 nm blue light inhibited cell proliferation and 660 nm red light stimulated cell proliferation while 390 nm ultraviolet radiation has little influence on cell proliferation. Furthermore, RNA-seq results showed that CSF1R, PPP3CC, ITGAL, ITGAM, IL2RB, and several other differentially expressed genes (DEGs) are involved in the cell proliferation. Relative DEGs values for matrix metalloproteinases (MMPs) gene family have shown a great difference in blue and red light radiation especially on MMP25, MMP9, MMP21, and MMP13.

Conclusion: Taken together, the results provide a valuable resource to describe the variation of HFs under PBM of different light at gene level.
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http://dx.doi.org/10.1111/phpp.12554DOI Listing
July 2020

Karacoline, identified by network pharmacology, reduces degradation of the extracellular matrix in intervertebral disc degeneration via the NF-κB signaling pathway.

J Pharm Anal 2020 Feb 26;10(1):13-22. Epub 2019 Jul 26.

Orthopedics Department, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine&Health Science, Shanghai, 201306, China.

Karacoline is a compound found in the plant Reichb. Although Reichb is widely used for the treatment of pain, very few studies have been carried out on the use of karacoline due to its potential toxicity. In this study, we selected key matrix metalloproteinases (MMPs), collagen II, and aggrecan as targets due to their association with intervertebral disc degeneration (IDD). Using these targets, we then used network pharmacology to predict a series of molecules that might exert therapeutic effects on IDD. Of these molecules, karacoline was predicted to have the best effect. Tumor necrosis factor (TNF)-α is known to promote the degeneration of the extracellular matrix in IDD. We therefore applied different concentrations of karacoline (0, 1.25, or 12.88 μM) along with 100 ng/mL TNF-α to rat nucleus pulposus cells and found that karacoline reduced the expression of MMP-14 in IDD by inhibiting the nuclear factor (NF)-κB pathway, while collagen II and aggrecan expression was increased. This suggested that extracellular matrix degradation was inhibited by karacoline ( < 0.05). Our data therefore reveal a new clinical application of karacoline and provide support for the use of network pharmacology in predicting novel drugs.
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http://dx.doi.org/10.1016/j.jpha.2019.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7037626PMC
February 2020

Visitor Engagement, Relationship Quality, and Environmentally Responsible Behavior.

Int J Environ Res Public Health 2020 02 12;17(4). Epub 2020 Feb 12.

Management School, Tianjin University of Commerce, Tianjin 300134, China.

Visitor environmentally responsible behavior (ERB) is helpful for promoting the sustainable development of tourist destinations. Existing studies on visitor ERB tend to either focus on restraining visitors' environmental misconducts or rely on visitors' psychological factors. Based on the theory of engagement, this paper constructs a theoretical model to explain visitors' self-conscious ERB. Visitor engagement with the destination is investigated as an independent variable which leads to the improvement of relationship quality and visitor ERB. Relationship quality is explored to mediate the impact of visitor engagement on ERB. In addition, the moderating role of environmental clue on the tested relationships is also examined. This study adopts partial least squares structural equation modeling (PLS-SEM) to investigate a total of 410 valid questionnaires. The results show that (1) visitor engagement with the destination positively affects visitor ERB; (2) relationship quality mediates the impact of visitor engagement on visitor ERB; (3) environmental clue plays a significant moderating role in the effects of engagement and relationship quality on visitor ERB. The study extends theoretical perspectives on visitor ERB and customer engagement, and provides managerially practical value to better understand visitors' self-conscious ERB.
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http://dx.doi.org/10.3390/ijerph17041151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7068390PMC
February 2020

Subchronic feeding toxicity studies of drought-tolerant transgenic wheat MGX11-10 in Wistar Han RCC rats.

Food Chem Toxicol 2020 Mar 11;137:111129. Epub 2020 Jan 11.

Tianjin Centers for Disease Control and Prevention, Tianjin, 300011, China. Electronic address:

A subchronic toxicity study were conducted in Wistar Han RCC rats to evaluate the potential health effects of genetically modified (GM), drought-tolerant wheat MGX11-10. Rats were fed a rodent diet formulated with MGX11-10 and were compared with rats fed a diet formulated with its corresponding non-transgenic control Jimai22 and rats fed a basal diet. MGX11-10 and Jimai22 were ground into flour and formulated into diets at concentrations of 16.25, 32.5, or 65%, w/w% and fed to rats (10/sex/group) for 13 weeks. Compared with rats fed Jimai22 and the basal-diet group, no biologically relevant differences were observed in rats fed the GM diet with respect to body weight/gain, food consumption/efficiency, clinical signs, mortality, ophthalmology, clinical pathology (hematology, prothrombin time, urinalysis, clinical chemistry), organ weights, and gross and microscopic pathology. Under the conditions of this study, the MGX11-10 diets did not cause any treatment-related effects in rats following at least 90 days of dietary administration as compared with rats fed diets with the corresponding non-transgenic control diet and the basal-diet group. The MGX11-10 diets are considered equivalent to the diets prepared from conventional comparators. The results demonstrated that MGX11-10 wheat is as safe and wholesome as the corresponding non-transgenic control wheat.
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http://dx.doi.org/10.1016/j.fct.2020.111129DOI Listing
March 2020

An aberrant metabolic flow toward early shunt products in the granaticin biosynthetic machinery of Streptomyces vietnamensis GIMV4.0001.

J Antibiot (Tokyo) 2020 04 10;73(4):260-264. Epub 2020 Jan 10.

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People's Republic of China.

A systematic study of the secondary metabolites of the wild granaticin-producing strain Streptomyces vietnamensis GIMV4.0001 led to the isolation of six known early shunt products related to actinorhodin, SEK34 (3), SEK34b (4), mutactin (5), dehydromutactin (7), EM18 (8) and GTRI-02 (9). While the other shunt products were minor or trace products, the production ratio of SEK34 (3) and SEK34b (4) to granaticins was strikingly high. Nearly 64% of the intermediate with the first ring closed went to the SEK34/SEK34b aberrant pathway. The high level of the aberrant metabolic flow toward the early shunt products SEK34 and SEK34b indicated that the second ring closure of the granaticin (1) biosynthesis is a key limiting step in the granaticin biosynthetic machinery of S. vietnamensis GIMV4.0001.
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http://dx.doi.org/10.1038/s41429-019-0267-8DOI Listing
April 2020

Family support for pregnant women with foetal abnormality requiring pregnancy termination in China.

Health Soc Care Community 2020 05 9;28(3):1020-1029. Epub 2020 Jan 9.

Department of nursing, Women's Hospital School of Medicine, Zhejiang University, Hangzhou, China.

Family support is an important protective factor for pregnant women with a foetal abnormality and can prevent adverse psychological outcomes in this population. This study aimed to explore the importance and influencing factors of family support for pregnant women with foetal abnormalities requiring pregnancy termination and then determine the correlation between family support and women's post-traumatic stress symptoms. A mixed methods study was conducted from March 2016 to September 2017. In all, 214 participants were surveyed using self-reported questionnaires including the demographic, family and obstetric information questionnaire, the Family Adaptation Partnership Growth Affection and Resolve Index and the Impact of Event Scale-Revised to collect quantitative data. Semi-structured in-depth interviews with 28 participants were conducted to collect qualitative data. Of the pregnant women with foetal abnormalities, 35% had obstacles in family function such that family support was low. The Impact of Event Scale-Revised score was negatively associated with total score on the Family Adaptation Partnership Growth Affection and Resolve Index as well as the score for each item on this index. In terms of family support, the demographic, family and obstetric variables hierarchically entered into the regression models significantly explained 20.0%, 26.5% and 2.6% of variation, respectively. In addition, three key themes were identified based on qualitative analyses: intensified instrumental support, inadequate emotional support and insufficient informational support. The findings showed that family support plays a protective role in preventing post-traumatic stress symptoms following termination of pregnancy. At present, family support still needs to be improved, especially in emotional support and informational support. The factors influencing family support may be important to consider for improving family support.
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http://dx.doi.org/10.1111/hsc.12935DOI Listing
May 2020

The Effects of Orientin on Proliferation and Apoptosis of T24 Human Bladder Carcinoma Cells Occurs Through the Inhibition of Nuclear Factor-kappaB and the Hedgehog Signaling Pathway.

Med Sci Monit 2019 Dec 14;25:9547-9554. Epub 2019 Dec 14.

Department of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).

BACKGROUND Orientin is a flavone isolated from medicinal plants used in traditional Chinese medicine (TCM), which suppresses the growth of cancer cells in vitro. The effects of orientin in bladder cancer cells remains unknown. This study aimed to investigate the effect of orientin on proliferation and apoptosis of T24 human transitional cell bladder carcinoma cells in vitro in the presence of an agonist and an inhibitor of nuclear factor-kappaB (NF-kappaB). MATERIAL AND METHODS T24 cells were cultured and divided into four study groups: an untreated control group; a group treated with 100 μM orientin; a group treated with 100 μM orientin with NF-kappaB agonist, phorbol 12-myristate 13-acetate (PMA); and a group treated with 100 μM orientin and the NF-kappaB inhibitor, IkappaBalpha. The MTT assay was performed to assess cell viability, and flow cytometry evaluated the cell cycle. The expression of proteins in the Hedgehog signaling pathway and inflammatory cytokines were determined by Western blot and enzyme-linked immunosorbent assay (ELISA). RESULTS Orientin inhibited the proliferation of T24 cells, caused cell cycle arrest, reduced cell viability, and inhibited the expression of inflammatory mediators. Treatment of T24 cells with orientin inhibited the expression of NF-kappaB and components of the Hedgehog signaling pathway, and the NF-kappaB agonist, PMA, reversed these effects. CONCLUSIONS Treatment of T24 human bladder carcinoma cells in vitro with orientin inhibited cell proliferation and promoted cell apoptosis by suppressing the Hedgehog signaling pathway and NF-kappaB.
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http://dx.doi.org/10.12659/MSM.919203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929549PMC
December 2019

Impact of Buckwheat Fermented Milk Combined with High-Fat Diet on Rats' Gut Microbiota and Short-Chain Fatty Acids.

J Food Sci 2019 Dec 27;84(12):3833-3842. Epub 2019 Nov 27.

School of Perfume and Aroma Technology, Shanghai Inst. of Technology, Shanghai, 201418, China.

The aim of this study was to evaluate the effect of buckwheat fermented milk on intestinal flora and short-chain fatty acids (SCFAs) of rats fed a high-fat diet (HFD). Buckwheat fermented milk was made with Lactobacillus plantarum ST-Ⅲ, Lactobacillus bulgaricus, and Streptococcus thermophilus. Thirty-six males C57BL/6 rats (aged 3 weeks and with 9 to 12 g weight) were subjected to a 2-week adaptive period on a normal diet. After a week of acclimatization, the rats were randomly divided into three groups with 12 rats in each group. The high-fat diet with fermented milk (HFDFM) group received HFD supplemented with fermented milk, and the high-fat diet supplemented with buckwheat fermented milk (HFDBFM) group received HFD plus buckwheat fermented milk. Besides, the gavage dose was 0.4 mL/day. After the feedings, colon levels of cytokines and antioxidant indices of the liver, colon, and duodenum tissues were measured. The composition of intestinal flora of rats and the content of SCFAs were determined by gas chromatography-mass spectrometry and Illumina high-throughput sequencing technology. Supplementation with buckwheat fermented milk significantly inhibited the increases in lipopolysaccharide levels in colon and antioxidant indexes in the HFD-fed rats. In addition, buckwheat fermented milk could significantly enhance Firmicutes and decrease Bacteroidetes compared to HFDFM. In addition, SCFA concentrations in HFDBFM were higher than other groups. In conclusion, buckwheat fermented milk can alleviate the damage of HFD to intestinal flora of rats. It modulates microbiota composition and SCFA concentrations to balance the intestinal environment, which will benefit to rats' intestinal health. PRACTICAL APPLICATION: This study explored the effect of buckwheat fermented milk on the regulation of intestinal microflora and SCFAs in rats fed a high-fat diet. It provides new ideas for the development of buckwheat fermented food.
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http://dx.doi.org/10.1111/1750-3841.14958DOI Listing
December 2019

Structural and antioxidant analysis of Tartary buckwheat (Fagopyrum tartaricum Gaertn.) 13S globulin.

J Sci Food Agric 2020 Feb 10;100(3):1220-1229. Epub 2019 Dec 10.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, China.

Background: The main component of buckwheat seed storage proteins is 13S globulin. In this study, Tartary buckwheat 13S globulin was separated and its structural features were investigated using Edman sequencing and matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS). The protective effect of its enzymatic hydrolysates against oxidative stress induced by H O was also evaluated to elucidate the antioxidant mechanism.

Results: Results showed that the isolated Tartary buckwheat 13S globulin contained one acidic and one basic subunit, which were linked by a disulfide bond. Six Tartary buckwheat active peptides were obtained from the enzymatic hydrolysates of Tartary buckwheat 13S globulin acidic subunit with a molecular weight of 38 kDa, namely Pep-1, Pep-2, Pep-3, Pep-4, Pep-5, and Pep-6. Pre-treatment of cells with Tartary buckwheat active peptides maintained the redox state balance of HepG cells and protected the activity of antioxidant enzymes in HepG cells. The Tartary buckwheat active peptides improved oxidative stress in HepG cells via the PPAR-α/HO-1 pathway.

Conclusion: These results provide an insight into the antioxidant mechanism of Tartary buckwheat 13S globulin and suggest that Tartary buckwheat active peptides can be used as a functional ingredient in the food industry. © 2019 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10133DOI Listing
February 2020

Expressive writing for Chinese women with foetal abnormalities undergoing pregnancy termination: An interview study of women's perceptions.

Midwifery 2019 Dec 1;79:102548. Epub 2019 Oct 1.

Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Objective: To investigate the effectiveness and applicability of expressive writing and women's experiences during the process.

Design And Setting: A descriptive qualitative study using a phenomenological approach was employed, and telephone interviews were conducted from February to April 2019 at a tertiary hospital in China.

Participants: The present study included 14 women with foetal abnormalities purposively sampled based on women's scores on the Impact of Event Scale-Revised one month after the completion of the expressive writing intervention.

Intervention: The participants were asked to write three 15-min essays (i.e. before, during, and after pregnancy termination) related to their experiences with the foetal abnormalities.

Findings: The transcripts were coded to generate themes and sub-themes to flow from the data. Four themes were revealed from the descriptions of practices of expressive writing during women's hospitalization: 1) benefits of expressive writing, 2) challenges in clinical practice, 3) suggestions for improvement and 4) future directions for development.

Key Conclusion: Expressive writing practices may contribute to the psychological health of women with foetal abnormalities undergoing pregnancy termination. Based on the research, the intervention of expressive writing was optimized to provide a basis for the formulation of psychological intervention strategies and the promotion of psychological rehabilitation for vulnerable women.

Implications For Practice: Expressive writing is an acceptable and feasible form of intervention for women with foetal abnormalities undergoing pregnancy termination.
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http://dx.doi.org/10.1016/j.midw.2019.102548DOI Listing
December 2019

genetic polymorphisms influence chronic obstructive pulmonary disease susceptibility in the Hainan population.

Int J Chron Obstruct Pulmon Dis 2019 5;14:2103-2115. Epub 2019 Sep 5.

Department of Respiratory Intensive Care Unit (RICU), The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 45000, People's Republic of China.

Introduction: Chronic obstructive pulmonary disease (COPD) is a lung disease closely related to exposure to exogenous substances. can activate many exogenous substances, which in turn affect lung cells. The aim of this study was to assess the association of single-nucleotide polymorphisms (SNPs) in with COPD risk in a Chinese Han population.

Materials And Methods: Genotypes of the five candidate SNPs in were identified among 318 cases and 508 healthy controls with an Agena MassARRAY method. The association between polymorphisms and COPD risk was evaluated using genetic models and haplotype analyses.

Results: In allele model, we observed that rs4803420 G and rs1038376 A were related to COPD risk. And rs4803420 G/T and G/T-T/T were related to a decreased COPD risk compared to GG genotype in the co-dominant and dominant models, respectively. When comparing with the AA genotype, rs1038376 A/T and A/T-T/T were associated with an increased COPD risk in the co-dominant and dominant models, respectively. Further gender stratification co-dominant and dominant models analysis showed that genotype G/T and G/T-T/T of rs4803420, and genotype A/T and A/T-T/T of rs1038376 were significantly associated with COPD risk compared to the wide type in males and females, while allele C of rs12979270 was only associated with COPD risk in females. Smoking status stratification analysis showed that rs12979270 C was significantly associated with an increased COPD risk under the allele model compared with allele A in the smoking subgroup. Haplotype analysis showed that haplotype GTA and TAA were related to COPD risk.

Conclusion: Our data is the first to demonstrate that polymorphisms may exert effects on COPD susceptibility in the Chinese Han population.
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http://dx.doi.org/10.2147/COPD.S214961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733340PMC
April 2020