Publications by authors named "Xiaoli Yang"

263 Publications

A complex of oxidised chitosan and silver ions grafted to cotton fibres with bacteriostatic properties.

Carbohydr Polym 2021 Jun 29;262:117714. Epub 2021 Jan 29.

Tianjin Key Laboratory of Pulp and Paper, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, Tianjin 300457, PR China. Electronic address:

The laccase/TEMPO system was employed to oxidise the C6 primary hydroxyl group on the chitosan (CS) to form a carboxyl group to obtain oxidised chitosan (C-COS). The silver-oxidised chitosan complex(C-COS-Ag) was prepared by reacting C-COS with silver nitrate, then C-COS-Ag and cotton fibres were subjected to a reaction to prepare bacteriostatic fibres. FT-IR and XPS analysis showed that: Ag and C-COS were combined in these forms: Ag, [Ag(NH)] OH, -COOAg, and AgO. C-COS-Ag was combined with cotton fibres by way of ester bonds. The inhibition zone of bacteriostatic fibres was all greater than 11 mm. After 50 washing tests, the bacteriostatic effect of bacteriostatic fibres remained at above 99 %. The amount of silver ions that had migrated from the bacteriostatic fibre was 3.336 mg/kg.
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http://dx.doi.org/10.1016/j.carbpol.2021.117714DOI Listing
June 2021

SARS-CoV-2 infection induces sustained humoral immune responses in convalescent patients following symptomatic COVID-19.

Nat Commun 2021 03 22;12(1):1813. Epub 2021 Mar 22.

Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Long-term antibody responses and neutralizing activities in response to SARS-CoV-2 infection are not yet clear. Here we quantify immunoglobulin M (IgM) and G (IgG) antibodies recognizing the SARS-CoV-2 receptor-binding domain (RBD) of the spike (S) or the nucleocapsid (N) protein, and neutralizing antibodies during a period of 6 months from COVID-19 disease onset in 349 symptomatic COVID-19 patients who were among the first be infected world-wide. The positivity rate and magnitude of IgM-S and IgG-N responses increase rapidly. High levels of IgM-S/N and IgG-S/N at 2-3 weeks after disease onset are associated with virus control and IgG-S titers correlate closely with the capacity to neutralize SARS-CoV-2. Although specific IgM-S/N become undetectable 12 weeks after disease onset in most patients, IgG-S/N titers have an intermediate contraction phase, but stabilize at relatively high levels over the 6 month observation period. At late time points, the positivity rates for binding and neutralizing SARS-CoV-2-specific antibodies are still >70%. These data indicate sustained humoral immunity in recovered patients who had symptomatic COVID-19, suggesting prolonged immunity.
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http://dx.doi.org/10.1038/s41467-021-22034-1DOI Listing
March 2021

Spatial Language of Young Children During Block Play in Kindergartens in Urban China.

Front Psychol 2021 23;12:568638. Epub 2021 Feb 23.

Faculty of Education, Beijing Normal University, Beijing, China.

Spatial language is an important predictor of spatial skills and might be inspired by peer interaction and goal-oriented building behaviors during block play. The present study investigated the frequency, type and level of children's spatial language during block play and their associations with the level of block play by observing 228 young children in classrooms equipped with unit blocks and allowing free play on a daily basis. The findings showed that during block play, young children used more words about spatial locations, deictic terms, dimensions, and shapes and fewer words about spatial features or properties and spatial orientations or transformations. Spatial locations were used most frequently, and young children tended to use vertical location words to represent the corresponding location. Most young children used gestures in conjunction with spatial deictic terms. Among shape words, tetragon words were frequently used, and the representation of spatial shapes showed alternatives, collective tendencies and gender differences. The use of spatial language during the play process had a significant positive correlation with age, the construction structure, and form of block building.
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http://dx.doi.org/10.3389/fpsyg.2021.568638DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940534PMC
February 2021

GASC1 promotes glioma progression by enhancing NOTCH1 signaling.

Mol Med Rep 2021 May 2;23(5). Epub 2021 Mar 2.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.

Recent studies have reported that gene amplified in squamous cell carcinoma 1 (GASC1) is involved in the progression of several types of cancer. However, whether GASC1 promotes glioma progression remains unknown. Therefore, the present study aimed to investigate the effect of GASC1 exposure on glioma tumorigenesis. The western blot demonstrated that grade III and IV glioma tissues exhibited a higher mRNA and protein expression of GASC1. Moreover, CD133+ U87 or U251 cells from magnetic cell separation exhibited a higher GASC1 expression. Invasion Transwell assay, clonogenic assay and wound healing assay have shown that GASC1 inhibition using a pharmacological inhibitor and specific short hairpin (sh)RNA suppressed the invasive, migratory and tumorsphere forming abilities of primary culture human glioma cells. Furthermore, GASC1‑knockdown decreased notch receptor (Notch) responsive protein hes family bHLH transcription factor 1 (Hes1) signaling. GASC1 inhibition reduced notch receptor 1 (NOTCH1) expression, and a NOTCH1 inhibitor enhanced the effects of GASC1 inhibition on the CD133+ U87 or U251 cell tumorsphere forming ability, while NOTCH1 overexpression abrogated these effects. In addition, the GASC1 inhibitor caffeic acid and/or the NOTCH1 inhibitor DAPT (a γ‑Secretase Inhibitor), efficiently suppressed the human glioma xenograft tumors. Thus, the present results demonstrated the importance of GASC1 in the progression of glioma and identified that GASC1 promotes glioma progression, at least in part, by enhancing NOTCH signaling, suggesting that GASC1/NOTCH1 signaling may be a potential therapeutic target for glioma treatment.
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http://dx.doi.org/10.3892/mmr.2021.11949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974312PMC
May 2021

Application of Near-Infrared Fluorescence Imaging Technology in Liver Cancer Surgery.

Surg Innov 2021 Feb 26:1553350621997777. Epub 2021 Feb 26.

Department of Hepatobiliary Surgery, 556508The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China.

Hepatocellular carcinoma, among the most common malignant digestive system tumorsworldwide, is most effectively treated with precise surgical resection. Near-infrared fluorescence imaging technology is being increasingly used clinically and has achieved great initial results in the navigation of liver cancer surgery. This review describes the application of indocyanine green fluorescence (ICG) imaging technology with near-infrared window I in the navigation of liver cancer surgery, explores novel fluorescent probes and near-infrared window II fluorescence imaging technology, and discusses the development status of the 2 emerging tools. ICG fluorescence imaging technology can precisely localize the tumor, reveal the boundary of liver cancer or liver segment, and identify the bile leakage. The novel fluorescent probe is more targeted than ICG, which makes the detection of cancer more accurate. Near-infrared window II fluorescence imaging technology can lead to outstanding gains in deeper detection, higher resolution, and fidelity. But, due to the shortcomings of machine and probe, it is not widely used in clinical. Near-infrared fluorescence imaging has great development potential. With the advent of precision medicine and the progress of various biotechnology studies, fluorescence imaging technology will be better developed and applied in the diagnosis, surgical navigation, and treatment of liver cancer.
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http://dx.doi.org/10.1177/1553350621997777DOI Listing
February 2021

Effects of Intergroup Vicarious Ostracism on Individual Prejudicial Attributions and Aggressive Intentions.

Span J Psychol 2021 Feb 23;24:e15. Epub 2021 Feb 23.

Gansu Normal University for Nationalities (China).

This study explores the effects of intergroup vicarious ostracism on individual prejudicial attributions and aggressive intentions. It takes Tibetan and Hui college students in northwestern China as participants. Study 1 and Study 2 explore the difference in observers' prejudicial attributions and aggressive intentions, respectively, when the group members who experienced ostracism (Tibetan college students) observed an in-group member being ostracized by out-group members versus an in-group member being ostracized by in-group members. Results show that those in-group participants, i.e., the Tibetan college students, who observed an in-group member being ostracized by out-group members, showed much higher prejudicial attributions, F(1, 106) = 19.65, p < .001, ηp2 = .156, and aggressive intentions, F(1, 108) = 10.51, p = .002, ηp2 = .089, toward ostracizers than those who observed an in-group member being ostracized by in-group members. In Study 3, Hui college students were recruited as participants to further test the results of Study 1 and Study 2. In addition, we also found that under the out-group conditions, prejudicial attribution mediates the effects of inclusionary status on aggressive intentions (95% bias-corrected confidence interval did not include zero; 95% CI [0.15, 0.69]). This study shows that ostracizers' group membership could affect observers' prejudicial attributions and their aggressive intentions toward the ostracizers.
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http://dx.doi.org/10.1017/SJP.2021.8DOI Listing
February 2021

Ursodeoxycholic Acid at 18-22 mg/kg/d Showed a Promising Capacity for Treating Refractory Primary Biliary Cholangitis.

Can J Gastroenterol Hepatol 2021 21;2021:6691425. Epub 2021 Jan 21.

Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University, Chengdu, China.

Aim: To compare the response between the current recommended dosage 13-15 mg/kg/d and 20 mg/kg/d dose of ursodeoxycholic acid (UDCA) in primary biliary cholangitis (PBC) patients who do not respond completely to a standard dose of UDCA.

Methods: We included 73 patients with poor response and randomized them into two groups to investigate whether increasing the dosage of UDCA was beneficial to nonresponders. Patients assigned to the 13-15 mg/kg/d group continued with standard therapy, and participants in the 18-22 mg/kg/d group switched to the higher dosage (18-22 mg/kg/d), with a follow-up of 12 months for both groups. The primary endpoints were the rate of response at 6 months and drug side effects.

Results: According to the Paris 2 criteria, patients receiving 18-22 mg/kg/d UDCA achieved a response rate of 59.4% compared with 36.1% in the standard dosage group (=0.046) at 6 months, respectively. At 12 months, the high-UDCA-dosage group achieved a response rate of 59.4% compared with 47.2% in the standard dosage group (=0.295), respectively. Additionally, the risk score predicted by the UK-PBC model was lower in high-dosage UDCA-treated patients than in the standard dosage group (all < 0.05). Side effects include diarrhea, nausea and vomiting, rash, and newly developed high blood pressure, which were mild and tolerated.

Conclusions: Patients treated with the high UDCA dosage showed some advantages over those who continued the standard dosage in terms of biochemical remission and disease progression, indicating that standard therapy with UDCA for 6 months and then another 1 year with high UDCA dosage for nonresponders could be a treatment option before second-line therapy is recommended.
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http://dx.doi.org/10.1155/2021/6691425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7843178PMC
January 2021

Delivery of toll-like receptor 3 ligand poly(I:C) to the liver by calcium phosphate nanoparticles conjugated with an F4/80 antibody exerts an anti-hepatitis B virus effect in a mouse model.

Acta Biomater 2021 Feb 1. Epub 2021 Feb 1.

Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1277, Wuhan 430022, P. R. China. Electronic address:

Hepatitis B virus (HBV) is a global health issue, but currently available anti-HBV drugs have limited success. Previously, introduction of the Toll-like receptor (TLR)-3 ligand poly(I:C) to the liver via hydrodynamic injection (HI) was shown to effectively suppress HBV replication in a chronic HBV replication mouse model. However, this method cannot be applied in human beings. To improve the liver targeting of poly(I:C) via intravenous injection, calcium phosphate nanoparticles (CPNs) carrying poly(I:C) with or without antibodies were constructed, and their anti-HBV effects were investigated. We found that significantly more anti-F4/80-conjugated and IgG2α-conjugated nanoparticles were taken up in liver cells both in vivo and in vitro. In addition, these nanoparticles produced pronounced immunostimulatory effects in vitro in primary liver cells. Importantly, treatment with nanoparticles carrying poly(I:C) increased the production of intrahepatic cytokines and chemokines and enhanced T cell responses, significantly reducing HBsAg, HBeAg and HBV DNA levels in the mice. Compared to nonconjugated and isotype-antibody-conjugated nanoparticles, the anti-F4/80-conjugated nanoparticles demonstrated the strongest anti-HBV effects. In summary, nanoparticles carrying poly(I:C) conjugated with an F4/80 antibody promoted liver targeting, and they may represent a suitable alternative to HI for future anti-HBV treatment. STATEMENT OF SIGNIFICANCE: HBV chronically infects approximately 250 million individuals worldwide but current anti-HBV drugs have limited success. Introduction of toll-like receptor 3 ligand poly(I:C) into liver by hydrodynamic injection has been proven to promote HBV clearance in mouse model. However, this technique is not clinically suitable for human patients. We have constructed calcium phosphate nanoparticles carrying poly(I:C) with specific antibody targeting liver nonparenchymal cells. The uptake into relevant liver cells and the anti-HBV effects were studied. After intravenous injection into mice, the uptake rate of anti-F4/80-conjugated nanoparticels was enhanced in liver, and these nanoparticles exert effective anti-HBV effects in vivo. This may provide important insight into future HBV immunotherapy based on nanoparticle-mediated drug delivery.
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http://dx.doi.org/10.1016/j.actbio.2021.01.045DOI Listing
February 2021

Neural stemness contributes to cell tumorigenicity.

Cell Biosci 2021 Jan 19;11(1):21. Epub 2021 Jan 19.

MOE Key Laboratory of Model Animals for Disease Study, and Model Animal Research Center of the Medical School, Nanjing University, 12 Xuefu Road, Pukou High-Tech Zone, Nanjing, 210061, China.

Background: Previous studies demonstrated the dependence of cancer on nerve. Recently, a growing number of studies reveal that cancer cells share the property and regulatory network with neural stem/progenitor cells. However, relationship between the property of neural stemness and cell tumorigenicity is unknown.

Results: We show that neural stem/progenitor cells, but not non-neural embryonic or somatic stem/progenitor cell types, exhibit tumorigenicity and the potential for differentiation into tissue types of all germ layers when they are placed in non-native environment by transplantation into immunodeficient nude mice. Likewise, cancer cells capable of tumor initiation have the property of neural stemness because of their abilities in neurosphere formation in neural stem cell-specific serum-free medium and in differentiation potential, in addition to their neuronal differentiation potential that was characterized previously. Moreover, loss of a pro-differentiation factor in myoblasts, which have no tumorigenicity, lead to the loss of myoblast identity, and gain of the property of neural stemness, tumorigenicity and potential for re-differentiation. By contrast, loss of neural stemness via differentiation results in the loss of tumorigenicity. These suggest that the property of neural stemness contributes to cell tumorigenicity, and tumor phenotypic heterogeneity might be an effect of differentiation potential of neural stemness. Bioinformatic analysis reveals that neural genes in general are correlated with embryonic development and cancer, in addition to their role in neural development; whereas non-neural genes are not. Most of neural specific genes emerged in typical species representing transition from unicellularity to multicellularity during evolution. Genes in Monosiga brevicollis, a unicellular species that is a closest known relative of metazoans, are biased toward neural cells.

Conclusions: We suggest that the property of neural stemness is the source of cell tumorigenicity. This is due to that neural biased unicellular state is the ground state for multicellularity and hence cell type diversification or differentiation during evolution, and tumorigenesis is a process of restoration of neural ground state in somatic cells along a default route that is pre-determined by an evolutionary advantage of neural state.
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http://dx.doi.org/10.1186/s13578-021-00531-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814647PMC
January 2021

RBM3 Increases Cell Survival but Disrupts Tight Junction of Microvascular Endothelial Cells in Acute Lung Injury.

J Surg Res 2021 May 15;261:226-235. Epub 2021 Jan 15.

Laboratory of Anesthesiology, Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China. Electronic address:

Background: RNA-binding motif protein 3 (RBM3) is an important cold shock protein, which also responds to hypothermia or hypoxia. RBM3 is involved into multiple physiologic processes, such as promoting cell survival. However, its expression and function in acute lung injury (ALI) have not been reported.

Methods: A mouse ALI model was established by lipopolysaccharides (LPS) treatment. The RBM3 and cold inducible RNA-binding protein mRNA levels were examined by RT-qPCR, and MMP9 mRNA stability was determined by actinomycin D assay. RBM3 and MMP9 mRNA was tested by RNA immunoprecipitation (RIP assay). RBM3 overexpression or silent stable cell lines were established using recombinant lentivirus and subsequently used for cell survival and tight junction measurements.

Results: In this study, we found that RBM3, rather than cold inducible RNA-binding protein, was upregulated in lung tissue of ALI mice. RBM3 was increased in human pulmonary microvascular endothelial cells (HPMVECs) in response to LPS treatment, which is modulated by the NF-κB signaling pathway. Furthermore, RBM3 could reduce cell apoptosis induced by LPS, probably through suppressing p53 expression. Because increased permeability of HPMVECs leads to pulmonary edema in ALI, we subsequently examined the effect of RBM3 on cell tight junctions. Unexpectedly, RBM3 decreased the expression of tight junction protein zonula occludens-1 and increased cell permeability, and RBM3 overexpression increased MMP9 mRNA stability. Furthermore, RIP assay confirmed the interaction between RBM3 and MMP9 mRNA, possibly explaining the contribution of RBM3 to increase cell permeability.

Conclusions: RBM3 seems to act as a "double-edged sword" in ALI, that RBM3 alleviates cell apoptosis but increases HPMVEC permeability in ALI.
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http://dx.doi.org/10.1016/j.jss.2020.12.041DOI Listing
May 2021

Patients with Osteoarthritis and Kashin-Beck Disease Display Distinct CpG Methylation Profiles in the , and Promoter Regions.

Cartilage 2021 Jan 16:1947603520988165. Epub 2021 Jan 16.

Institute of Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Objective: We aimed to analyze deoxycytidine-deoxyguanosine dinucleotide (CpGs) methylation profiles in , and promoter regions in osteoarthritis (OA) and Kashin-Beck disease (KBD) patients.

Methods: Blood samples were collected from 16 primary OA patients and corresponding 16 healthy individuals and analyzed for methylations in the CpGs of , and promoter regions using MALDI-TOF-MS. The methylation profiles of these regions were then compared between OA and KBD patients.

Results: -1_CpG_2 and -1_CpG_3 methylations were significantly lower in OA than KBD patients ( < 0.05). A similar trend was observed for -1_CpG_4, -1_CpG_7, -1_CpG_8.9.10, -1_CpG_13.14.15 and -1_CpG_16 ( < 0.05) as well as -1_CpG_1 and -1_CpG_2 methylation between OA and KBD patients ( < 0.05). However, there was no difference in methylation levels of other CpGs between the 2 groups ( > 0.05).

Conclusion: OA and KBD patients display distinct methylation profiles in the CpG sites of , and promoter regions. These findings provide a strong background and new perspective for future studies on mechanisms underlying epigenetic regulation of selenoprotein genes associated with OA and KBD diseases.
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http://dx.doi.org/10.1177/1947603520988165DOI Listing
January 2021

miR‑142‑3p targets AC9 to regulate sciatic nerve injury‑induced neuropathic pain by regulating the cAMP/AMPK signalling pathway.

Int J Mol Med 2021 Feb 16;47(2):561-572. Epub 2020 Dec 16.

Department of Orthopedics, Zaozhuang Hospital of Zaozhuang Mining Group, Zaozhuang, Shandong 277100, P.R. China.

The aim of the present study was to investigate the effects of microRNA (miR)‑142‑3p on neuropathic pain caused by sciatic nerve injury in chronic compression injury (CCI) rats, and further investigate its mechanism. Rat experiments were divided into four parts in the study. In the first part, the rats were divided into the Sham and CCI groups. The expression of miR‑142‑3p, AC9 and cAMP were detected. In the second part, the rats were divided into the Sham, CCI, miR‑142‑3p mimic, mimic‑negative control (NC), miR‑142‑3p small interfering RNA (siRNA) and siRNA‑NC groups. The expression of cAMP and the levels of AMPK pathway‑related proteins were detected. In the third part, the rats were randomly divided into Sham, CCI, AC9 mimic, mi‑NC, AC9 siRNA and si‑NC groups. Double luciferase reporter assay was used to analyse the targeting relationship between miR‑142‑3p and AC9. In the fourth part, the rats were divided into the Sham, CCI, miR‑142‑3p siRNA, AC9 mimic, miR‑142‑3p siRNA + AC9 siRNA, cAMP activator (Forskolin) and miR‑142‑3p siRNA + cAMP inhibitor groups. The expression of miR‑142‑3p was significantly increased while AC9 and cAMP expression significantly decreased in CCI rats. However, AC9 overexpression significantly increased the levels of cAMP protein. Luciferase reporter assay also proved that AC9 is the target gene of miR‑142‑3p. Moreover, miR‑142‑3p silencing was found to reduce neuropathic pain in CCI rats by upregulating the expression of AC9. It was also found that cAMP activation can relieve neuropathic pain and promote the expression of AMPK‑related proteins in CCI rats. Silencing miR‑142‑3p can target AC9 to reduce the expression of inflammatory factors and neuropathic pain in CCI rats by increasing the expression of cAMP/AMPK pathway‑related proteins.
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http://dx.doi.org/10.3892/ijmm.2020.4824DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797458PMC
February 2021

Characterization of the complete mitochondrial genome of the Meiren yak ().

Mitochondrial DNA B Resour 2020 Feb 11;5(1):1102-1104. Epub 2020 Feb 11.

Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, People's Republic of China.

In this study, high-throughput Illumina sequencing was employed to assemble the complete mitochondrial genome of the Meiren yak (), a local yak breed from Gansu Province, China. The mitochondrial genome is 16,321 bp long with an A + T-biased nucleotide composition and harbors 13 protein-coding, 22 Trna, and 2 rRNA genes, and a noncoding control region. The mitogenomic organization and codon usage are highly similar to those of previously published congeneric mitochondrial genomes. Bayesian phylogenetic analysis indicates that Meiren yak is most closely related to nine other yak breeds (incl. Datong, Huanhu, Pali, Pamir, Polled, Qilian, Seron, Sunan, and Tianjun yaks).
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http://dx.doi.org/10.1080/23802359.2020.1726222DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748641PMC
February 2020

Insulin Resistance is Significantly Related with Worse Clinical Outcomes in Non-Diabetic Acute Ischemic Stroke Patients Treated with Intravenous Thrombolysis.

J Stroke Cerebrovasc Dis 2021 Mar 16;30(3):105526. Epub 2020 Dec 16.

Department of Neurology, Shanghai Fifth People's Hospital, Fudan University, Shanghai, China; Department of Neurology, Shanghai Ninth People's Hospital(North Part), Shanghai Jiaotong University, Shanghai, China. Electronic address:

Objectives: to investigate the relationship between insulin resistance (IR) and clinical outcomes in non-diabetic ischemic stroke patients treated with intravenous thrombolysis.

Methods: We recruited non-diabetic ischemic stroke patients treated with intravenous thrombolysis prospectively. IR was defined as homeostasis model assessment-estimated insulin resistance index ≥2.80. Initial stroke severity was assessed using the National Institutes of Health Stroke Scale scores, and infarct volume was measured using DWI. Clinical outcomes were evaluated by neurological improvement and hemorrhagic transformation at 24 hours, and favorable functional prognosis at 90 days.

Results: 232 patients were enrolled into this study. IR group was 67 patients, non-IR group was 165 patients. Compared with the non-IR group, the probability of neurological improvement at 24 h ours and favorable functional outcome at 90 days in IR group were all significantly lower (41.79% vs 63.03%, p<0.01; 73.13% vs 89.09%, p<0.01 respectively), whereas the ratio of hemorrhagic transformation was much higher (16.42% vs 4.85%, p<0.01). In multivariable logistic regression, IR was negatively associated with neurological improvement and favorable functional prognosis (OR=0.39, 95%CI, 0.20-0.76, p<0.01; OR= 0.26, 95%CI, 0.07-0.91, p=0.04, respectively), but was positively correlated with hemorrhagic transformation (OR=4.07, 95%CI, 1.13-14.59, p=0.03) after adjusting traditional risk factors. We analyzed 108 infarct volume data further, the median of volume in IR group was 2.27 cm, higher than that in non-IR group (1.96 cm), but no statistical difference (p=0.65).

Conclusions: In non-diabetic ischemic stroke patients treated with intravenous thrombolysis, IR was related with worse clinical outcomes, but not with infarct volume.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105526DOI Listing
March 2021

Clinical Efficacy of Perioperative Immunonutrition Containing Omega-3-Fatty Acids in Patients Undergoing Hepatectomy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Ann Nutr Metab 2020 11;76(6):375-386. Epub 2020 Dec 11.

Department of Hepatobiliary Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, China,

Background: The effect of immunonutrition in patients undergoing hepatectomy remains unclear. This meta-analysis aimed to assess the impact of immunonutrition on postoperative clinical outcomes in patients undergoing hepatectomy.

Methods: A literature search of PubMed, Cochrane Library, Web of Science, and Embase databases was performed to identify all randomized controlled trials (RCTs) exploring the effect of perioperative immunonutrition in patients undergoing hepatectomy until the end of March 10, 2020. Quality assessment and data extraction of RCTs were conducted independently by 3 reviewers. Mean difference (MD) and odds ratio (OR) with 95% confidence interval (CI) were calculated using a fixed-effects or random-effects model. The meta-analysis was performed with RevMan 5.3 software.

Results: Nine RCTs involving a total of 966 patients were finally included. This meta-analysis showed that immunonutrition significantly reduced the incidences of overall postoperative complications (OR = 0.57, 95% CI: 0.34-0.95; p = 0.03), overall postoperative infectious complications (OR = 0.53, 95% CI: 0.37-0.75; p = 0.0003), and incision infection (OR = 0.50, 95% CI: 0.28-0.89; p = 0.02), and it shortened the length of hospital stay (MD = -3.80, 95% CI: -6.59 to -1.02; p = 0.007). There were no significant differences in the incidences of pulmonary infection (OR = 0.60, 95% CI: 0.32-1.12; p = 0.11), urinary tract infection (OR = 1.30, 95% CI: 0.55-3.08; p = 0.55), liver failure (OR = 0.54, 95% CI: 0.23-1.24; p = 0.15), and postoperative mortality (OR = 0.69, 95% CI: 0.26-1.83; p = 0.46).

Conclusion: Given its positive impact on postoperative complications and the tendency to shorten the length of hospital stay, perioperative immunonutrition should be encouraged in patients undergoing hepatectomy.
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http://dx.doi.org/10.1159/000509979DOI Listing
December 2020

Effects of Dexmedetomidine on Emergence Agitation and Recovery Quality Among Children Undergoing Surgery Under General Anesthesia: A Meta-Analysis of Randomized Controlled Trials.

Front Pediatr 2020 13;8:580226. Epub 2020 Nov 13.

Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Emergence agitation (EA) is one of the most common and intractable postoperative complications among children undergoing surgery under general anesthesia. Dexmedetomidine, an α(2)-adrenoceptor agonist, offers an ideal sedation, reduces preoperative anxiety, and facilitates smooth induction of anesthesia, and it is widely used in pediatric surgery. We aimed to evaluate the efficacy of dexmedetomidine for preventing emergence agitation in children after general anesthesia. We comprehensively reviewed PubMed, Cochrane Library, EMBASE, and Web of Science databases to search all randomized controlled trials, published before April 22, 2020, investigating the efficacy of dexmedetomidine in preventing the emergence agitation in children after general anesthesia. The meta-analysis was performed using Review Manager 5.3. The primary outcome was the incidence of emergence agitation. Secondary outcomes included the number of patients requiring rescue analgesic, number of patients with postoperative nausea and vomiting, emergence time, extubation time, and time to discharge from the post-anesthesia care unit. We included a total of 33 studies, comprising 2,549 patients in this meta-analysis. Compared with saline, dexmedetomidine significantly reduced the emergence agitation incidence [risk ratio (RR) 0.29; 95% confidence interval (CI) 0.22-0.37; < 0.00001], incidence of postoperative nausea and vomiting (RR 0.46; 95% CI 0.3-0.69; = 0.0002), and the requirement of rescue analgesic (RR 0.29; 95% CI 0.18-0.44; < 0.00001). Furthermore, children in the dexmedetomidine group experienced a longer emergence time [mean difference (MD) 2.18; 95% CI 0.81-3.56; = 0.002] and extubation time (MD 0.77; 95% CI 0.22-1.31; = 0.006) compared with those in the saline group. However, no significant difference was observed in the time to discharge from the post-anesthesia care unit (MD 2.22; 95% CI -2.29-6.74; = 0.33) between the two groups. No significant differences were observed between the effects of dexmedetomidine and other drugs like midazolam, propofol, fentanyl, tramadol, and clonidine in terms of the emergence agitation incidence and other parameters, except for the requirement of rescue analgesic (RR 0.45; 95% CI 0.33-0.61; < 0.00001). Dexmedetomidine can prevent emergence agitation, relieves postoperative pain, decreases the requirement of rescue analgesic, and decreases the postoperative nausea and vomiting events.
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http://dx.doi.org/10.3389/fped.2020.580226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7694572PMC
November 2020

HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry.

Nat Metab 2020 12 26;2(12):1391-1400. Epub 2020 Nov 26.

Beijing Institute of Biotechnology, Academy of Military Medical Sciences (AMMS), Beijing, China.

Responsible for the ongoing coronavirus disease 19 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infects host cells through binding of the viral spike protein (SARS-2-S) to the cell-surface receptor angiotensin-converting enzyme 2 (ACE2). Here we show that the high-density lipoprotein (HDL) scavenger receptor B type 1 (SR-B1) facilitates ACE2-dependent entry of SARS-CoV-2. We find that the S1 subunit of SARS-2-S binds to cholesterol and possibly to HDL components to enhance viral uptake in vitro. SR-B1 expression facilitates SARS-CoV-2 entry into ACE2-expressing cells by augmenting virus attachment. Blockade of the cholesterol-binding site on SARS-2-S1 with a monoclonal antibody, or treatment of cultured cells with pharmacological SR-B1 antagonists, inhibits HDL-enhanced SARS-CoV-2 infection. We further show that SR-B1 is coexpressed with ACE2 in human pulmonary tissue and in several extrapulmonary tissues. Our findings reveal that SR-B1 acts as a host factor that promotes SARS-CoV-2 entry and may help explain viral tropism, identify a possible molecular connection between COVID-19 and lipoprotein metabolism, and highlight SR-B1 as a potential therapeutic target to interfere with SARS-CoV-2 infection.
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http://dx.doi.org/10.1038/s42255-020-00324-0DOI Listing
December 2020

Expression of the erythropoietin receptor in patients with proliferative diabetic retinopathy and its correlation with postoperative visual prognosis.

Ann Palliat Med 2020 Nov 18;9(6):4010-4016. Epub 2020 Nov 18.

Department of Ophthalmology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Background: Proliferative diabetic retinopathy (PDR) is a leading cause of blindness. This study aimed to analyze the expression of the erythropoietin receptor (EpoR) in patients with PDR and its correlation with postoperative visual prognosis.

Methods: Between May 2016 and May 2017, 89 patients who underwent vitreoretinal surgery (VRS) in Affiliated Hospital of North Sichuan Medical College were enrolled. Among them, 45 patients (52 eyes) with PDR and 44 patients (50 eyes) with retinal vein occlusion (RVO) made up the study group and the control group, respectively. All patients received 25G standard flattened three-channel lens-preserving VRS; all procedures were performed by the same physician. Recovery and change in best corrected visual acuity (BCVA) were observed. Subsequently, the patients were divided into group A (improved vision) and group B (no improvement in vision). The integrated optical density (IOD) value of EpoR expression among the groups were compared. Pearson's correlation analysis was used to analyze the correlation between the IOD values of EpoR and the change in visual acuity after surgery for PDR.

Results: The IOD value of EpoR in the study group was higher than that in the control group (P<0.05). The postoperative BCVA of the PDR patients was significantly higher than that before surgery (≥0.1 vs. ≤0.02, P<0.05). Of the 45 PDR patients (52 eyes), 34 patients (39 eyes) had improved visual acuity after surgery (group A), while 11 patients (13 eyes) experienced no improvement (group B). The IOD value of EpoR expression in group B was higher than that in group A (P<0.05). Pearson's correlation analysis showed that the IOD value of EpoR expression were positively correlated with the recovery.

Conclusions: EpoR is expressed at a high level in PDR patients. The expression level of EpoR in the epiretinal membrane of PDR patients is positively correlated with the prognosis of postoperative vision. Therefore, EpoR has promise as a prognostic biomarker and a potential therapeutic target for PDR.
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http://dx.doi.org/10.21037/apm-20-1898DOI Listing
November 2020

Estrogen receptor α/prolactin receptor bilateral crosstalk promotes bromocriptine resistance in prolactinomas.

Int J Med Sci 2020 23;17(18):3174-3189. Epub 2020 Oct 23.

Department of Neurosurgery, Zhongnan hospital of Wuhan university, Wuhan, Hubei 430071, P.R. China.

Prolactinomas are the most common type of functional pituitary adenoma. Although bromocriptine is the preferred first line treatment for prolactinoma, resistance frequently occurs, posing a prominent clinical challenge. Both the prolactin receptor (PRLR) and estrogen receptor α (ERα) serve critical roles in the development and progression of prolactinomas, and whether this interaction between PRLR and ERα contributes to bromocriptine resistance remains to be clarified. In the present study, increased levels of ERα and PRLR protein expression were detected in bromocriptine-resistant prolactinomas and MMQ cells. Prolactin (PRL) and estradiol (E2) were found to exert synergistic effects on prolactinoma cell proliferation. Furthermore, PRL induced the phosphorylation of ERα via the JAK2-PI3K/Akt-MEK/ERK pathway, while estrogen promoted PRLR upregulation via pERα. ERα inhibition abolished E2-induced PRLR upregulation and PRL-induced ERα phosphorylation, and fulvestrant, an ERα inhibitor, restored pituitary adenoma cell sensitivity to bromocriptine by activating JNK-MEK/ERK-p38 MAPK signaling and cyclin D1 downregulation. Collectively, these data suggest that the interaction between the estrogen/ERα and PRL/PRLR pathways may contribute to bromocriptine resistance, and therefore, that combination treatment with fulvestrant and bromocriptine (as opposed to either drug alone) may exert potent antitumor effects on bromocriptine-resistant prolactinomas.
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http://dx.doi.org/10.7150/ijms.51176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646122PMC
October 2020

Pimozide augments bromocriptine lethality in prolactinoma cells and in a xenograft model via the STAT5/cyclin D1 and STAT5/Bcl‑xL signaling pathways.

Int J Mol Med 2021 Jan 5;47(1):113-124. Epub 2020 Nov 5.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China.

As hyperprolactinemia is observed in patients with bromocriptine‑resistant prolactinoma, prolactin (PRL) has been implicated in the development of bromocriptine resistance. Since PRL primarily mediates cell survival and drug resistance via the Janus kinase‑2 (JAK2)/signal transducer and activator of transcription 5A (STAT5) signaling pathway, the STAT5 inhibitor, pimozide, may inhibit cell proliferation and reverse bromocriptine resistance in prolactinoma cells. In the present study, compared with bromocriptine or pimozide alone, the combination of pimozide and bromocriptine exerted enhanced reduction in cell growth and proliferation, and increased apoptosis and cell cycle arrest in bromocriptine‑resistant prolactinoma cells. A reduction in phospho‑STAT5, cyclin D1 and B‑cell lymphoma extra‑large (Bcl‑xL) expression levels were observed in cells treated with the combination of drugs. In addition, pimozide suppressed spheroid formation of human pituitary adenoma stem‑like cells, and reduced the protein expression of the cancer stem cell markers, CD133 and nestin. Pimozide did not exert any additional antitumor activity in STAT5‑knockdown primary culture cells of human bromocriptine‑resistant prolactinomas. Furthermore, Pimozide combined with bromocriptine treatment significantly reduced human prolactinoma xenograft growth. Western blot and immunohistochemical analyses also demonstrated significant inhibition of cell proliferation and stem cell marker proteins in vivo. Collectively, these data indicated that pimozide treatment reduced prolactinoma growth by targeting both proliferating cells and stem cells, at least in part, by inhibiting the STAT5/Bcl‑xL and STAT5/cyclin D1 signaling pathways.
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http://dx.doi.org/10.3892/ijmm.2020.4784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723514PMC
January 2021

The Type III Secretion Effector IpaH4.5 Targets NLRP3 to Activate Inflammasome Signaling.

Front Cell Infect Microbiol 2020 30;10:511798. Epub 2020 Sep 30.

Department of Genetic Engineering, Beijing Institute of Biotechnology, Beijing, China.

Activation of the NLRP3 inflammasome requires the expression of NLRP3, which is strictly regulated by its capacity to directly recognize microbial-derived substances. Even though the involvement of caspase-1 activation in macrophages NLRP3 and NLRC4 has been discovered, the accurate mechanisms by which infection triggers NLRP3 activation remain inadequately understood. Here, we demonstrate that IpaH4.5, a T3SS effector, triggers inflammasome activation by regulating NLRP3 expression through the E3 ubiquitin ligase activity of IpaH4.5. First, we found that IpaH4.5 interacted with NLRP3. As a result, IpaH4.5 modulated NLRP3 protein stability and inflammasome activation. Bacteria lacking IpaH4.5 had dramatically reduced ability to induce pyroptosis. Our results identify a previously unrecognized target of IpaH4.5 in the regulation of inflammasome signaling and clarify the molecular basis for the cytosolic response to the T3SS effector.
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http://dx.doi.org/10.3389/fcimb.2020.511798DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7561375PMC
September 2020

Increased levels of angiotensin Ⅱ type 1 receptor autoantibodies in female infertility.

Syst Biol Reprod Med 2020 Oct 18:1-8. Epub 2020 Oct 18.

Reproductive Department of Taiyuan Central Hospital, Taiyuan Central Hospital of Shanxi Medical University , Taiyuan, China.

In this study, the level of serum anti-angiotensin II type 1 receptor autoantibodies (AT1-AA) was determined. It was found that the positive rate of AT1-AA in serum of infertile women is higher than that of healthy non-pregnant women. Spearman correlation analysis showed that AT1-AA was negatively correlated with oocyte maturation rate (r = -0.29, P < 0.01) and AT1-AA was positively correlated with IL-6 (r = 0.68, P < 0.01). Logistic regression analysis showed that age, BMI, type of infertility, years of infertility, history of poor pregnancy outcome, condition of fallopian tube, and polycystic ovary syndrome were not factors influencing the level of AT1-AA in the serum of infertile patients. The results indicated that AT1-AA was involved in the pathological changes of infertile women. AT1-AA may be related to oocyte maturation disorder, ovulation disorder. Interestingly it appears to induce an inflammatory reaction, although the specific mechanism is not clear. However, the level of AT1-AA is not affected by individual differences in infertile patients. : E:estradiol; P:progesterone; RAS:Renin-angiotensin system; Ovras:renin-angiotensin system; ACE1:angiotensin-converting enzyme-1; AngII:angiotensin II; AT1R:Angiotensin II type 1 Receptor; BMI:Body Mass Index; AT1-A:anti-angiotensin II type 1 receptor autoantibodies.
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http://dx.doi.org/10.1080/19396368.2020.1829172DOI Listing
October 2020

[A Light Adaptive Heart Rate Detection Method Based on Webcam].

Zhongguo Yi Liao Qi Xie Za Zhi 2020 Oct;44(5):377-383

Lab of Medical Artificial Intelligence, School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, 471000.

In order to extract the pulse wave signal of blood volume effectively in the case of uneven light, a light-adaptive heart rate detection method based on webcam was proposed. In this method, adaptive gamma transform is applied to face image sequence to eliminate the influence of illumination. The pulse wave source signal was extracted from the forehead area and the blood volume pulse wave was obtained by wavelet filtering. The heart rate is estimated by Fourier transform analysis. The Bland-Altman analysis indicates that the method used in this paper is in good agreement with the measurement results of the electronic sphygmomanometer, and the adaptive gamma transformation used in this paper eliminates the influence of light interference, and the measurement error of heart rate is significantly reduced, which is completely able to meet the requirements of daily heart rate monitoring.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2020.05.001DOI Listing
October 2020

Characteristics of Myelogram in Patients with Extraocular Metastatic Retinoblastoma and Morphological Analysis of Tumor Cells in Bone Marrow and Cerebrospinal Fluid.

Ophthalmologica 2020 Oct 12. Epub 2020 Oct 12.

Objectives: To investigate the myelogram characteristics of RB with extraocular tumor extension and the morphological characteristics of tumor cells in bone marrow and cerebrospinal fluid.

Methods: The clinical data of 18 cases in our hospital from May 2011 to February 2015 diagnosed with metastatic RB in the extraocular and other distant regions associated with clear bone marrow metastasis were analyzed. The morphology of tumor cells in bone marrow and cerebrospinal fluid were retrospectively analyzed after staining with Wright-Giemsa stain. A summary of the cytological characteristics was also presented.

Results: RB tumor cells in bone marrow and cerebrospinal fluid not only appeared as aggregated clumps, but also was distributed in a scattered manner. The tumor cells may present different characteristic morphologies in different cases, even the different tumor cell smears made from the same tumor mass may show different morphological features. According to the degree of tumor metastasis, changes in myelogram were significantly different.

Conclusion: The tumor cells of RB patients show unique morphological characteristics in bone marrow and cerebrospinal fluid. Therefore, correct identification of the cells is of great value in the diagnosis, staging and prognosis of RB.
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http://dx.doi.org/10.1159/000512193DOI Listing
October 2020

Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway.

Int Immunopharmacol 2020 Dec 8;89(Pt A):107014. Epub 2020 Oct 8.

College of Pharmacy, Guangxi Medical University, Nanning, Guangxi, China. Electronic address:

Acute lung injury (ALI) is a life-threatening disorder with high rates of morbidity and mortality. Up to now, there are still no effective drugs for its therapies due to the complexity of its etiology and pathogenesis. In this present study, we investigated the protective effect of Nervilifordin F (NF) on ALI induced by intestinal ischemia/reperfusion (II/R) and its related mechanism. Firstly, the ALI model rats were induced through II/R, and treated with NF. Then, the pathological and cytokine level changes in the lung tissue of ALI rats were evaluated by hematoxylin and eosin and enzyme-linked immunosorbent assay (ELISA). The related genes expression level of mammalian target of rapamycin (mTOR) pathway and inflammasome were measured by real-time quantitative polymerase chain reaction (RT-qPCR), western blot and immunohistochemistry. Finally, the NF-protein complexes were predicted by SYBYL-X 2.0. The results indicated that NF can significant reduces the levels of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6 and IL-1β, and inhibits the expression of inflammasome related genes (such as toll-like receptor 4 (TLR4), p65, NOD-like receptor protein 3 (NLRP3) and Caspase 1), thereby reduce inflammation in II/R-induced ALI rats. Moreover, NF can activate the expression of FK506 binding protein 25 (FKBP25) and down-regulate the expression of mTOR and p70 ribosomal protein S6 kinase 1 (p70S6K). In addition, molecular docking results showed that NF can be combined well with p70S6K, TLR4, mTOR and NLRP3, which further verified the inhibitory effect of NF on ALI inflammation. Therefore, the findings indicate that NF can alleviates II/R-induced inflammation of ALI rats by inhibiting inflammasome related genes and mTOR pathway, which expected to use as a potential drug for the treatment of ALI.
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http://dx.doi.org/10.1016/j.intimp.2020.107014DOI Listing
December 2020

Diabetes and PKM2 affect prognosis in patients with intrahepatic cholangiocarcinoma.

Oncol Lett 2020 Nov 21;20(5):265. Epub 2020 Sep 21.

Department of Hepatobiliary Surgery, Traditional Chinese Medicine Hospital of Chongqing Dianjiang, Chongqing 408300, P.R. China.

Diabetes mellitus (DM) is a common disease, but its effect on the prognosis of patients with intrahepatic cholangiocarcinoma (ICC) has not been reported. The aim of the present study was to explore the prognostic significance of diabetes in patients with ICC treated with hepatectomy and to clarify the role of pyruvate kinase M2 (PKM2). A consecutive retrospective cohort of 110 patients with ICC (28 with DM and 82 without DM) who underwent therapeutic hepatectomy was evaluated between January 2006 and January 2011. The clinicopathological characteristics of the two groups and the differences between overall survival (OS) and recurrence-free survival (RFS) were analyzed. The Cox proportional hazards model was further used to identify independent prognostic predictors. PKM2 expression was measured using immunohistochemical staining in tissues collected, after obtaining informed consent. Patients with ICC with DM exhibited significantly lower OS and RFS rates at 1, 3 and 5 years compared with patients with ICC without DM. Cox multivariate analysis revealed that DM was an independent predictor of poor OS and RFS. Additionally, high PKM2 expression was significantly higher in patients with ICC with DM compared with that in patients without DM. Overall, DM was associated with significantly lower OS and RFS rates in patients with ICC. The underlying biological rationale may be attributed to the higher PKM2 expression rate.
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http://dx.doi.org/10.3892/ol.2020.12128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7517629PMC
November 2020

Comparison of outcomes in intrauterine insemination, fertilisation and intracytoplasmic sperm injection in men with and without varicocele.

Int J Med Sci 2020 31;17(14):2155-2162. Epub 2020 Jul 31.

Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics; The Key Laboratory for Reproductive Endocrinology of Ministry of Education, Jinan, Shandong 250021, P.R. China.

To investigate the differences in clinical pregnancy, miscarriage, and live birth rates when male partners were diagnosed with a varicocele and to compare these outcomes to those without and study the outcomes based on the grade of varicocele. The retrospective study was based on a cohort of consecutive infertile couples undergoing assisted reproductive technology (ART) at the Reproductive Center of Shandong Provincial Hospital affiliated to the Shandong University during the period between January 2017 and December 2018. A total of 4203 couples comprised of men with and without varicocele undergoing the first ART cycle (1501 intrauterine inseminations (IUI), 1623 fertilisations (IVF) and 1079 intracytoplasmic sperm injections (ICSI)) were included. Semen parameters and ART outcomes were determined. ICSI (26.5%) originated from men with a significant lower level in sperm concentration and motility but with a strict normal morphology had a higher prevalence of varicocele than men undergoing IUI (20.7%) and IVF (18.1%). In IUI, the odds ratios (ORs) for pregnancy and live birth were significantly lower for couples in men diagnosed with grades 1 or 2 varicocele as compared to those for men with grade 3 varicocele. In IVF, ORs for live birth where men were diagnosed with grades 1 or 2 varicocele were also lower than those for men with grade 3,whereas a higher miscarriage rate was found when men had grades 1 or 2 varicocele than when men had grade 3. However, for ICSI, no significant outcomes were found in grades 1, 2 or 3 varicocele versus the no varicocele group. The increasing grade of varicocele was negatively associated with sperm parameters and can alter the outcome of further IUI/IVF.
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http://dx.doi.org/10.7150/ijms.48005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7484661PMC
July 2020

Dynamics analysis of the hippocampal neuronal model subjected to cholinergic action related with Alzheimer's disease.

Cogn Neurodyn 2020 Aug 1;14(4):483-500. Epub 2020 Apr 1.

Department of Applied Mathematics, Northwestern Polytechnical University, Xi'an, 710072 People's Republic of China.

There are evidences that the region of hippocampus is affected in the early stage of Alzheimer's disease (AD). Moreover, the hippocampal pyramidal neurons receive cholinergic input from the medial septum. Thus, this study, based on the results of electrophysiological experiments, first constructs a modified hippocampal CA1 pyramidal neuronal model by introducing two new currents of M-current and calcium ion-activated potassium ion current to depict the cholinergic input receiving from the medial septum, and then explores how acetylcholine deficiency and beta-amyloid accumulation under the pathological condition of AD influence the neuronal dynamics in terms of theta band power and spiking frequency using computational approach. By simulating acetylcholine potentiated M-current and calcium ion-activated potassium ion current, numerical results reveal that the relative theta band power increases significantly and the firing rate decreases obviously when acetylcholine is deficient. Similarly, by simulating beta-amyloid enhanced delay rectification potassium ion current, we also detect that the relative theta band power increases as well as the firing rate decreases remarkably as beta-amyloid is accumulated. In addition, the mechanism underlying these dynamical changes in theta rhythm and firing behavior is investigated by nonlinear behavioral analysis, which demonstrates that both deficiency in acetylcholine and accumulation in beta-amyloid can promote the emergence of stable equilibrium state in this modified hippocampal neuronal model. Note that acetylcholine deficiency together with beta-amyloid deposition plays key role in the pathogenesis of AD. We expect these findings could have important implications on better understanding pathogenesis and expounding potential biomarkers for AD.
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http://dx.doi.org/10.1007/s11571-020-09586-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334339PMC
August 2020

Identification of differentially expressed and methylated genes associated with rheumatoid arthritis based on network.

Autoimmunity 2020 09 10;53(6):303-313. Epub 2020 Jul 10.

Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P.R. China.

Rheumatoid arthritis (RA) is a multi-systemic inflammatory autoimmune disease involving peripheral joints, and the pathogenesis is not clear. Studies showed that DNA methylation and expression might also be involved in the pathogenesis of RA. This study integrated three expression profile datasets (GSE55235, GSE12021, and GSE55457) and one methylation profile dataset GSE111942 to elucidate the potential essential candidate genes and pathways in RA. Differentially expressed genes (DEGs) and differentially methylation genes (DMGs) were identified by R programming software, using Limma package and ChAMP package, respectively. DAVID performed gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs. Functional annotation and construction of a protein-protein interaction (PPI) network and the Molecular Complex Detection Algorithm (MCODE) were analysed by STRING and Cystoscope, respectively. Then the connection analysis of DEGs and DMGs was carried out, and further to analyse the relationship between methylation and gene expression, aiming to screen out the potential genes. In this study, 288 DEGs and 228 DMGs were identified, and the majority of DEGs were up-regulated. Enrichment analysis represented that DEGs mainly involved immune response and participated in the Cytokine-cytokine receptor interaction signal pathway. 282 nodes were identified from DEGs PPI network and MCODE, filtering the most significant 2 modules, 23 core node genes were identified and most of them are involved in the T cell receptor signalling pathway and chemokine-mediated signalling pathway. Cross-analysis revealed 4 genes [ (cg 01277763), (cg 07600884), (cg 05961700), and (cg 05205664)] that exhibited differential expression and methylation in RA simultaneously. Therefore, the four genes could be used as the target for RA.
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http://dx.doi.org/10.1080/08916934.2020.1786069DOI Listing
September 2020

Enhanced character-level deep convolutional neural networks for cardiovascular disease prediction.

BMC Med Inform Decis Mak 2020 07 9;20(Suppl 3):123. Epub 2020 Jul 9.

College of Computer Science and Engineering, Northwest Normal University, 967 Anning East Road, Lanzhou, 730070, China.

Background: Electronic medical records contain a variety of valuable medical information for patients. So, when we are able to recognize and extract risk factors for disease from EMRs of patients with cardiovascular disease (CVD), and are able to use them to predict CVD, we have the ability to automatically process clinical texts, resulting in an improved accuracy of supporting doctors for the clinical diagnosis of CVD. In the case where CVD is becoming more worldwide, predictive CVD based on EMRs has been studied by many researchers to address this important aspect of improving diagnostic efficiency.

Methods: This paper proposes an Enhanced Character-level Deep Convolutional Neural Networks (EnDCNN) model for cardiovascular disease prediction.

Results: On the manually annotated Chinese EMRs corpus, our risk factor identification extraction model achieved 0.9073 of F-score, our prediction model achieved 0.9516 of F-score, and the prediction result is better than the most previous methods.

Conclusions: The character-level model based on text region embedding can well map risk factors and their labels as a unit into a vector, and downsampling plays a crucial role in improving the training efficiency of deep CNN. What's more, the shortcut connections with pre-activation used in our model architecture implements dimension-matching free in training.
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http://dx.doi.org/10.1186/s12911-020-1118-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346321PMC
July 2020