Publications by authors named "Xiaoli Wang"

953 Publications

Effect of Various Laser-Activated Irrigation Protocols on Endodontic Sealer Penetration into the Dentinal Tubules: A Confocal Laser Scanning Microscopy Study.

Photobiomodul Photomed Laser Surg 2021 Jul 30. Epub 2021 Jul 30.

State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, P.R. China.

To study the effect of various laser-activated irrigation protocols on endodontic sealer penetration into the dentinal tubules. Laser-activated irrigation is helpful for smear layer removal and thus might increase the penetration of endodontic sealer into the dentinal tubules. Forty-eight mandibular premolars with straight canals were instrumented and divided into four groups: C-I group, conventional needle irrigation with sodium hypochlorite (NaOCl) followed by ethylenediaminetetraacetic acid (EDTA); Er:YAG-I, Er,Cr:YSGG-I, and Nd:YAP-I groups, NaOCl and EDTA irrigations activated by erbium:yttrium-aluminum-garnet (Er:YAG), erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG), and neodymium:yttrium-aluminum-perovskite (Nd:YAP) lasers, respectively. The cleanness of the treated canals was evaluated by scanning electron microscopy (SEM). Then, canals were obturated with AH-Plus sealer labeled with rhodamine B (0.1%) and combined with gutta-percha. After setting, canals were sectioned at 2, 5, and 8 mm short from the root apex, and the penetration area of the AH-Plus into the dentinal tubules was observed using confocal laser scanning microscopy (CLSM). SEM results indicated that the Er:YAG-I and Er,Cr:YSGG-I lasers were more effective than the Nd:YAP-I and C-I in removing the smear layer. CLSM results indicated that the average AH-Plus penetration areas among the tested groups were in ascending order: C-I < Nd:YAP-I < Er:YAG-I, Er,Cr:YSGG-I ( < 0.05). Laser-activated irrigations significantly increased the penetration of the endodontic sealer into the dentinal tubules, among which Er:YAG-I and Er,Cr:YSGG-I lasers were the most effective and may be considered routine protocols for endodontic treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/photob.2020.4924DOI Listing
July 2021

Thermal-Driven Dynamic Shape Change of Bimetallic Nanoparticles Extends Hot Electron Lifetime of Pt/MoS Catalysts.

J Phys Chem Lett 2021 Jul 26:7173-7179. Epub 2021 Jul 26.

College of Chemistry, Key Laboratory of Theoretical & Computational Photochemistry of Ministry of Education, Beijing Normal University, Beijing 100875, P. R. China.

Using a combination of time-domain density functional theory and nonadiabatic (NA) molecular dynamics, we demonstrate that the replacement of noble Pt with cheap Sn in the Pt nanoparticles sensitized MoS greatly retards the photoexcited "hot" electron relaxation. The simulations show that Sn substitution causes significant geometry distortion associated with the Sn dopant detaching from the Pt nanoparticle base, which decreases the NA coupling and creates an isolated trap state distant from the electron donor state. Generally, smaller NA coupling delays "hot" electron relaxation. At the same time, the photoexcited electron on MoS first populates the nanoparticles state and then slowly goes to the trap state, following relaxation to the nanoparticle acceptor state over 1 ps. As a result, the "hot" electron lives over 3.5 times longer than that in pristine Pt/MoS system. The long-lived "hot" electron associated with the reduced cost establishes a novel concept for developing high-efficient and cost-effective photocatalysts and photovoltaics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.1c01640DOI Listing
July 2021

Preparation of a Gangue-Based X-type Zeolite Molecular Sieve as a Multiphase Fenton Catalyst and Its Catalytic Performance.

ACS Omega 2021 Jul 2;6(28):18414-18425. Epub 2021 Jul 2.

Inner Mongolia Key Laboratory of Environmental Chemistry, College of Chemistry and Environmental Science, Inner Mongolia Normal University, Hohhot 010022, China.

In this study, a series of X-type zeolite molecular sieve catalysts, modified with copper (Cu-X), were prepared by an alkali fusion-hydrothermal synthesis using coal gangue from Inner Mongolia. These catalysts were used in the degradation of the methylene blue dye by a Fenton-like reaction. Characterization results showed that Cu is considered to be present in the surface structure of the zeolite in the form of doped Cu ions and metal oxide. It is believed that Cu is the main active site involved in the Fenton reaction. The X-ray photoelectron spectroscopy (XPS) spectra indicated that Cu and Cu coexist in the catalysts and participate together in the Fenton reaction. The degradation of methylene blue by the Cu-X catalysts was investigated to determine the optimal catalytic conditions in terms of six aspects: catalyst dosage, initial solution concentration, initial pH of the solution, HO dosage, copper loading, and reaction temperature. The experimental results showed that CX-1.0 had excellent activity and stability for the degradation and decolorization of the methylene blue dye, which could completely degrade the dye within 90 min, and the total organic carbon removal rate reached as high as 97.8%. Electron spin resonance (ESR) and radical capture experiments showed that OH played a dominant role in the Fenton-like reaction. Combined with XPS, ESR, and catalytic tests, the redox cycle of Cu/Cu was found to be accelerating the generation of reactive radicals in the Fenton system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c02469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296578PMC
July 2021

Validation and Extrapolation of a Multimodal Infection Prevention and Control Intervention on Carbapenem-Resistant in an Epidemic Region: A Historical Control Quasi-Experimental Study.

Front Med (Lausanne) 2021 7;8:692813. Epub 2021 Jul 7.

Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

To verify the effects of comprehensive infection prevention and control (IPC) interventions for the prevention of the cross-transmission of carbapenem-resistant (CRKP) within intensive care units (ICUs) in an epidemic region. A historical control, quasi-experimental design was performed. The study was conducted between January 2017 and December 2019, following the implementation of a multimodal IPC bundle. The baseline period was established from January 2013 to June 2013, when only basic IPC measures were applied. A total of 748 patients were enrolled during the entire study. The incidence of ICU-acquired CRKP colonization/infection was 1.16 per 1,000 patient-days during the intervention period, compared with 10.19 per 1,000 patient-days during the baseline period ( = 0.002). The slope of the monthly incidence of CRKP at admission showed an increasing trend ( = 0.03). The incidence of ICU-acquired catheter-related bloodstream infections caused by CRKP decreased from 2.54 to 0.96 per 1,000 central-line-days ( = 0.08). Compliance with contact precautions and terminal room disinfection improved during the intervention period. All environmental surface culture samples acquired after terminal room disinfection were negative for CRKP. Our findings suggest that in epidemic settings, multimodal IPC intervention strategies and consistent monitoring of compliance, may limit the spread of CRKP in ICUs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmed.2021.692813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292674PMC
July 2021

Bursal peptide BP-IV as a novel immunoadjuvant enhances the protective efficacy of an epitope peptide vaccine containing T and B cell epitopes of the H9N2 avian influenza virus.

Microb Pathog 2021 Jul 16;158:105095. Epub 2021 Jul 16.

The Key Lab of Veterinary Biological Products, Henan University of Science and Technology, Luoyang, Henan, China. Electronic address:

Short peptide antigens covering conserved T or B cell epitopes have been investigated in influenza vaccines. Bursal pentapeptide V (BPP-V) and bursal peptide IV (BP-IV) are small molecular peptides that were isolated and identified from the bursa of Fabricius (BF) and induce a strong immune response at both the humoural and cellular levels. To explore the molecular adjuvant potential of BPP-V and BP-IV with an epitope vaccine, an epitope peptide (HA, GNCVVQCQTERGGLN) rich in T and B cell epitopes for the H9N2 avian influenza virus (AIV) haemagglutinin (HA) protein was selected. BPP-V and BP-IV were coupled with the epitope peptide sequence to form BPP-V and BP-IV-epitope vaccines, respectively. The immunoefficacy of BPP-V and BP-IV-epitope peptide vaccines was evaluated. The results showed that the epitope peptide had weak immunogenicity. The BPP-V-epitope peptide vaccine promoted only the secretion of anti-HA IgG and IgG1 antibodies. The BP-IV-epitope peptide vaccine not only promoted the production of anti-HA IgG and IgG1 antibodies but also significantly induced the production of the IgG2a antibody. The BP-IV-epitope peptide vaccine significantly promoted the production of interleukin (IL-4) and interferon-γ (IFN-γ) (the BPP-V epitope peptide vaccine promoted only the production of IL-4), enhanced the cytotoxic T lymphocyte (CTL) response, and increased the proportion of CD3 T lymphocytes. Moreover, the BP-IV-epitope peptide vaccine promoted a cell-mediated immune response similar to that of the AIV vaccine group. Furthermore, BPP-V and BP-IV-epitope peptide vaccines could also accelerate the clearance of pulmonary virus and reduce pathological damage after the challenge with H9N2 AIV. This study demonstrates the potential of BP-IV as an effective adjuvant for the epitope peptide vaccine for the H9N2 AIV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.micpath.2021.105095DOI Listing
July 2021

Malondialdehyde in dried blood spots: a biomarker of systemic lipid peroxidation linked to cardiopulmonary symptoms and risk factors.

J Thorac Dis 2021 Jun;13(6):3731-3740

Nicholas School of the Environment & Duke Global Health Institute, Duke University, Durham, NC, USA.

Background: There are few oxidative biomarkers that can be used in resource-limited settings (e.g., rural Africa) where blood collection facilities are lacking. This study aims to evaluate the potential of malondialdehyde (MDA) in dried blood spots (DBS) as a useful biomarker to monitor cardiopulmonary health.

Methods: We first conducted a cross-validation comparison of matched capillary DBS, plasma, and whole venous blood collected from nine healthy volunteers for the measurement of total MDA (free + conjugated) and C-reactive protein (CRP), a well-established biomarker of systemic inflammation. Then a field study was conducted in a rural Senegal with a population of 441 women routinely exposed to severe household air pollution, examining associations of MDA and CRP levels in 882 DBS with self-reported cardiopulmonary symptoms.

Results: In the cross-validation study, CRP levels were strongly correlated across DBS, plasma, and whole blood. MDA levels were correlated between DBS and whole blood and were 1-2 orders of magnitude lower in plasma, suggesting that DBS MDA may reflect total oxidation levels in intracellular and extracellular compartments. In the field study, we observed significantly higher MDA levels in women with secondhand smoke exposure. An interquartile range increase in MDA concentration was associated with 27.0% (95% CI: 3.1-56.5%) and 21.1% (95% CI: -3.5% to 52.0%) increases in the incidence of chest tightness and breath difficulty, respectively. In contrast, CRP levels were not associated with worse outcomes or risk factors.

Conclusions: These results support the use of DBS as a convenient alternative to venous blood when MDA is measured as a biomarker for cardiopulmonary health risk.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/jtd-21-604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264672PMC
June 2021

Antibiotics in mariculture organisms of different growth stages: Tissue-specific bioaccumulation and influencing factors.

Environ Pollut 2021 Jul 7;288:117715. Epub 2021 Jul 7.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China. Electronic address:

Maricultured organisms are chronically exposed to water containing antibiotics but the bioaccumulative behavior of antibiotics in exposed organisms at different growth stages has received little attention. Here, we investigated the concentrations and tissue-specific bioaccumulation characteristics of 19 antibiotics during three growth stages (youth stage, growth stage, and adult stage) of various organisms (Scophthalmus maximus, Penaeus vannamei, Penaeus japonicus, and Apostichopus japonicus) cultivated in typical marine aquaculture regions, and explored the factors that could affect the bioaccumulation of antibiotics. Tetracyclines (TCs) and fluoroquinolones (FQs) were the dominant antibiotics in all organisms, and the total concentrations of the target antibiotics in fish (S. maximus) were significantly higher than those in shrimp (P. vannamei and P. japonicus) and sea cucumber (A. japonicus) (p < 0.01). The bioaccumulation capacity of a class of statistically significant antibiotics in most samples was strongest during the youth stage and weakest during the adult stage. The antibiotics exhibited higher bioaccumulation capacity in lipid-rich tissues (fish liver and shrimp head) or respiratory organs (fish gill) than muscle. Our results also reveal significant metabolic transformation of enrofloxacin in fish. Different from previous studies, the logarithm bioaccumulation factor (log BAF) was positively correlated with log D in low-biotransformation tissues (fish gill and muscle) rather than lipid-rich tissues (fish liver). Based on the calculated hazard quotients (HQ), doxycycline in fish muscle may pose a distinct risk to human health, which deserves special attention. Overall, these results provide insight into the bioaccumulation patterns of antibiotics during different growth stages and tissues of maricultured organisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.envpol.2021.117715DOI Listing
July 2021

The complete chloroplast genome sequence of , a widespread grass in Tibet, China.

Mitochondrial DNA B Resour 2021 28;6(8):2105-2106. Epub 2021 Jun 28.

Qinghai Academy of Animal and Veterinary Science/State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

The complete chloroplast genome of was sequenced and reported here. The total genome size was 138,294 bp in length, containing a large single-copy region of 81,229 bp, a small single-copy region of 12,419 bp, and a pair of inverted repeat regions of 22,288 bp. The GC content of chloroplast genome was 38.6%. It encodes a total of 119 unique genes, including 81 protein-coding genes, 34 tRNA genes, and four rRNA genes. Phylogenetic analysis showed a strong sister relationship with and . Our findings provide fundamental information for further evolutionary and phylogenetic researches of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2021.1903355DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245088PMC
June 2021

Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean.

Plant Methods 2021 Jul 10;17(1):73. Epub 2021 Jul 10.

Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.

Background: Agrobacterium-mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic transformation is still recalcitrant and time-consuming, hampering the high-throughput functional analysis of soybean genes. Thus we pursue to develop a rapid, simple, and highly efficient hairy root system induced by Agrobacterium rhizogenes (A. rhizogenes) to analyze soybean gene function.

Results: In this report, a rapid, simple, and highly efficient hairy root transformation system for soybean was described. Only sixteen days were required for the whole workflow and the system was suitable for various soybean genotypes, with an average transformation frequency of 58-64%. Higher transformation frequency was observed when wounded cotyledons from 1-day-germination seeds were inoculated and co-cultivated with A. rhizogenes in 1/2 B5 (Gamborg' B-5) medium. The addition of herbicide selection to root production medium increased the transformation frequency to 69%. To test the applicability of the hairy root system for gene functional analysis, we evaluated the protein expression and subcellular localization in transformed hairy roots. Transgenic hairy roots exhibited significantly increased GFP fluorescence and appropriate protein subcellular localization. Protein-protein interactions by BiFC (Bimolecular Fluorescent Complimentary) were also explored using the hairy root system. Fluorescence observations showed that protein interactions could be observed in the root cells. Additionally, hairy root transformation allowed soybean target sgRNA screening for CRISPR/Cas9 gene editing. Therefore, the protocol here enables high-throughput functional characterization of candidate genes in soybean.

Conclusion: A rapid, simple, and highly efficient A. rhizogenes-mediated hairy root transformation system was established for soybean gene functional analysis, including protein expression, subcellular localization, protein-protein interactions and gene editing system evaluation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13007-021-00778-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272327PMC
July 2021

Recognition of Fetal Facial Ultrasound Standard Plane Based on Texture Feature Fusion.

Comput Math Methods Med 2021 3;2021:6656942. Epub 2021 Jun 3.

Biomedical Ultrasound Laboratory, The University of Southern California (USC), Los Angeles, USA.

In the process of prenatal ultrasound diagnosis, accurate identification of fetal facial ultrasound standard plane (FFUSP) is essential for accurate facial deformity detection and disease screening, such as cleft lip and palate detection and Down syndrome screening check. However, the traditional method of obtaining standard planes is manual screening by doctors. Due to different levels of doctors, this method often leads to large errors in the results. Therefore, in this study, we propose a texture feature fusion method (LH-SVM) for automatic recognition and classification of FFUSP. First, extract image's texture features, including Local Binary Pattern (LBP) and Histogram of Oriented Gradient (HOG), then perform feature fusion, and finally adopt Support Vector Machine (SVM) for predictive classification. In our study, we used fetal facial ultrasound images from 20 to 24 weeks of gestation as experimental data for a total of 943 standard plane images (221 ocular axial planes, 298 median sagittal planes, 424 nasolabial coronal planes, and 350 nonstandard planes, OAP, MSP, NCP, N-SP). Based on this data set, we performed five-fold cross-validation. The final test results show that the accuracy rate of the proposed method for FFUSP classification is 94.67%, the average precision rate is 94.27%, the average recall rate is 93.88%, and the average 1 score is 94.08%. The experimental results indicate that the texture feature fusion method can effectively predict and classify FFUSP, which provides an essential basis for clinical research on the automatic detection method of FFUSP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6656942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195636PMC
June 2021

Effect of tranexamic acid on clinical therapeutic effect and blood coagulation function of high-risk pregnant women with postpartum hemorrhage after cesarean section.

Minerva Med 2021 Jun 28. Epub 2021 Jun 28.

Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China -

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0026-4806.21.07616-3DOI Listing
June 2021

Surfactant-Stripped Micelles with Aggregation-Induced Enhanced Emission for Bimodal Gut Imaging In Vivo and Microbiota Tagging Ex Vivo.

Adv Healthc Mater 2021 Jun 23:e2100356. Epub 2021 Jun 23.

School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300350, P. R. China.

Aggregation-induced emission luminogens (AIEgens) hold promise for biomedical imaging and new approaches facilitating their aggregation state are desirable for fluorescence enhancement. Herein, a series of surfactant-stripped AIEgen micelles (SSAMs) with improved fluorescence are developed by a low-temperature surfactant-stripping method to encapsulate AIEgens in temperature-sensitive Pluronic block copolymer. After stripping excessive surfactant, SSAMs exhibit altered optical properties and significantly higher fluorescence quantum yield. Using this method, a library of highly concentrated fluorescent nanoparticles are generated with tunable absorption and emission wavelengths, permitting imaging of deep tissues at different wavelengths. SSAMs remain physiologically stable and can pass safely through gastrointestinal tract (GI) without degradation in the harsh conditions, allowing for fluorescence and photoacoustic imaging of intestine with high resolution. d-amino acids (DAA), a natural metabolite for bacteria, can be chemically conjugated on the surface of SSAMs, enabling non-invasive monitoring of the microbial behavior of ex vivo fluorescently labeled gut microbiota in the GI tract.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adhm.202100356DOI Listing
June 2021

Altered miR-93-5p/miR-18a expression in serum for diagnosing non-small cell lung cancer.

Am J Transl Res 2021 15;13(5):5073-5079. Epub 2021 May 15.

Department of Medical Oncology, Affiliated Tumor Hospital of Nantong University Nantong 226361, Jiangsu Province, China.

Objective: This research aimed at probing into miR-93-5p and miR-18a's diagnostic and prognostic values in non-small cell lung cancer (NSCLC) patients.

Methods: A total of 107 patients diagnosed with NSCLC in the Department of Oncology and Thoracic Surgery of our hospital from January 2015 to June 2016 were regarded as the research group (RG), and 42 healthy people were considered as the control group (CG). Serum samples were collected and miR-93-5p, miR-18a expression was detected via qPCR. The relationship between miR-93-5p, miR-18a and clinicopathological characteristics of NSCLC patients was assessed, and the diagnostic value of the two miRNAs was analyzed by ROC curve.

Results: miR-93-5p and miR-18a were up-regulated in NSCLC. The higher the degree of tumor differentiation, the higher the TNM stage and the expression of the two miRNAs were. The high expression was tied to tumor differentiation degree, TNM stage, lymph node metastasis and lymph-vascular space invasion (LVSI). The survival rate of miR-93-5p and miR-18a high expression patients was worse than that of those with low expression. The AUC value of both of the mRNAs in NSCLC diagnosis was high (0.8905).

Conclusion: The expression of miR-93-5p and miR-18a is associated with NSCLC severity and prognosis, and both can be used as potential markers for diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205697PMC
May 2021

Glycosylation of β1 subunit plays a pivotal role in the toxin sensitivity and activation of BK channels.

J Venom Anim Toxins Incl Trop Dis 2021 Jun 2;27:e20200182. Epub 2021 Jun 2.

Department of Neurology and Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: The accessory β1 subunits, regulating the pharmacological and biophysical properties of BK channels, always undergo post-translational modifications, especially glycosylation. To date, it remains elusive whether the glycosylation contributes to the regulation of BK channels by β1 subunits.

Methods: Herein, we combined the electrophysiological approach with molecular mutations and biochemical manipulation to investigate the function roles of N-glycosylation in β1 subunits.

Results: The results show that deglycosylation of β1 subunits through double-site mutations (β1 N80A/N142A or β1 N80Q/N142Q) could significantly increase the inhibitory potency of iberiotoxin, a specific BK channel blocker. The deglycosylated channels also have a different sensitivity to martentoxin, another BK channel modulator with some remarkable effects as reported before. On the contrary to enhancing effects of martentoxin on glycosylated BK channels under the presence of cytoplasmic Ca, deglycosylated channels were not affected by the toxin. However, the deglycosylated channels were surprisingly inhibited by martentoxin under the absence of cytoplasmic Ca, while the glycosylated channels were not inhibited under this same condition. In addition, wild type BK (α+β1) channels treated with PNGase F also showed the same trend of pharmacological results to the mutants. Similar to this modulation of glycosylation on BK channel pharmacology, the deglycosylated forms of the channels were activated at a faster speed than the glycosylated ones. However, the V and slope were not changed by the glycosylation.

Conclusion: The present study reveals that glycosylation is an indispensable determinant of the modulation of β1-subunit on BK channel pharmacology and its activation. The loss of glycosylation of β1 subunits could lead to the dysfunction of BK channel, resulting in a pathological state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1590/1678-9199-JVATITD-2020-0182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183112PMC
June 2021

Reversal of Abnormal CD4+ T Cell Metabolism Alleviates Thyroiditis by Deactivating the mTOR/HIF1a/Glycolysis Pathway.

Front Endocrinol (Lausanne) 2021 4;12:659738. Epub 2021 Jun 4.

Department of Endocrinology and Metabolism, The Endocrine Institute and The Liaoning Provincial Key Laboratory of Endocrine Diseases, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Hospital of China Medical University, Shenyang, China.

Background: Hashimoto's thyroiditis (HT) is an autoimmune disease that features activation of thyroid antigen-specific helper T cells. HT patients have increased Th1 and Th17 T cell subsets. Glycolysis supports chronic activation of Th1 and Th17 T cells, but how this contributes to HT remains unknown.

Methods: The metabolism of CD4+ T cells from 30 HT patients and 30 healthy controls was evaluated by determining the extracellular acidification rate (ECAR) and the oxygen consumption rate (OCR). Mice in a subacute thyroiditis (SAT) model were treated with 2DG, metformin, or combination. Metrics of mTOR/HIF-1α/HK2/glycolysis were measured by western blot and Seahorse assay methods. The severity of SAT was measured by flow cytometry and HE staining.

Results: CD4+ T cells from HT patients had enhanced ECAR and OCR. Levels of Glut1, HK2, PKM2, and LDHA in cultured HT CD4+ T cells were elevated. The expression of HK2 and PKM2 in cultured SAT CD4+ T cells was elevated compared with the control group. Activation of the mTOR and HIF-1α pathways was significant in SAT mice, and expression of HIF-1α in the 2DG treated group was reduced. Treatment with 2DG and/or metformin significantly decreased the ratio of Th17 and Th1 T cells.

Conclusions: Thyroiditis results in elevation of the mTOR/HIF-1α/HK2/glycolysis pathway in CD4+ T cells. The activation of this pathway is reduced by treatment with 2DG and metformin, which also reverted imbalances in CD4+ T cell differentiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fendo.2021.659738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211914PMC
June 2021

Effects of Organic Polymer Compound Material on K and Na Distribution and Physiological Characteristics of Cotton Under Saline and Alkaline Stresses.

Front Plant Sci 2021 28;12:636536. Epub 2021 May 28.

Agricultural College, Shihezi University, Shihezi, China.

Soil salinization and alkalization greatly restrict crop growth and yield. In this study, NaCl (8 g kg) and NaCO (8 g kg) were used to create saline stress and alkaline stress on cotton in pot cultivation in the field, and organic polymer compound material (OPCM) and stem girdling were applied before cotton sowing and at flowering and boll-forming stage, respectively, aiming to determine the effects of OPCM on K and Na absorption and transport and physiological characteristics of cotton leaf and root. The results showed that after applying the OPCM, the Na content in leaf of cotton under saline stress and alkaline stress were decreased by 7.72 and 6.49%, respectively, the K/Na ratio in leaf were increased by 5.65 and 19.10%, respectively, the Na content in root were decreased by 9.57 and 0.53%, respectively, the K/Na ratio in root were increased by 65.77 and 55.84%, respectively, and the transport coefficients of K and Na from leaf to root were increased by 39.59 and 21.38%, respectively. The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), and the relative electrical conductivity (REC) in cotton leaf were significantly increased, while the content of malondialdehyde (MDA) was decreased; but the changes in those in root were not significant. The boll weights were increased by 11.40 and 13.37%, respectively, compared with those for the control. After stem girdling, the application of OPCM still promoted the ion transport of cotton organs; moreover, the CAT activity in root was increased by 25.09% under saline stress, and the SOD activity in leaf and CAT in root were increased by 42.22 and 6.91%, respectively under alkaline stress. Therefore, OPCM can significantly change the transport of K and Na to maintain the K and Na homeostasis in leaf and root, and regulate physiological and biochemical indicators to alleviate the stress-induced damage. Besides, the regulation effect of OPCM on saline stress was better than that on alkaline stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.636536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194489PMC
May 2021

Probiotics alleviate adipose inflammation in high-fat diet-induced obesity by restoring adipose invariant natural killer T cells.

Nutrition 2021 Apr 25;89:111285. Epub 2021 Apr 25.

Department of Endocrinology and Metabolism, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China.

Objective: Invariant natural killer T (iNKT) cells, which are depleted in obese individuals, play important roles in preventing diet-induced obesity and associated disorders. Probiotic supplementation can alter the gut microbiota and immunomodulation in obesity. However, it remains unclear whether probiotics can affect visceral adipose iNKT cells. The aim of this study was to analyze the effects of probiotics on adipose iNKT cells in mice with high-fat diet (HFD)-induced obesity and to assess the immunomodulatory function of probiotics and their role in obesity, glucose tolerance, lipid metabolism, insulin resistance, and adipose inflammation.

Methods: Wildtype (WT) male C57BL/6 mice and CD1d knockout mice were fed an HFD or a normal-fat diet. Some mice received active or heat-sacrificed VSL#3 probiotics. Preventative VSL#3 therapy was also administered to HFD mice. Body weight, metabolic parameters, expression of genes encoding adipose inflammatory factors (interleukin [IL]-4, IL-10, tumor necrosis factor-α, interferon-γ, and IL-6), adipose iNKT cell frequency, and subphenotype were evaluated.

Results: HFD induced more severe obesity in CD1dKO mice than in WT mice. VSL#3 intervention significantly improved HFD-induced weight gain, adipose iNKT cell depletion, and metabolic and adipose inflammatory profiles in WT mice, but not in CD1dKO mice. Preventative VSL#3 treatment improved HFD-induced obesity and metabolic parameters, and elevated total adipose iNKT and IL-4 iNKT cell frequencies.

Conclusions: Probiotic intervention alleviated weight gain, improved metabolic parameters, and reduced adipose inflammation in HFD-induced obesity. These effects seem to depend on the restoration of visceral adipose iNKT cells. These findings have potential implications for the management of obesity-related diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nut.2021.111285DOI Listing
April 2021

Identification of Susceptible Genes for Chronic Obstructive Pulmonary Disease with Lung Adenocarcinoma by Weighted Gene Co-Expression Network Analysis.

Onco Targets Ther 2021 4;14:3625-3634. Epub 2021 Jun 4.

Department of Respiratory and Critical Care Medicine, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.

Purpose: Chronic obstructive pulmonary disease (COPD) and lung adenocarcinoma (LUAD) are common disorders and usually co-exists. However, genetic mechanisms between COPD and LUAD are rarely reported. This study aims to identify susceptible genes of COPD with LUAD.

Methods: Using the published data of GSE106899, co-expression modules were constructed by weighted gene co-expression network analysis (WGCNA). Subsequently, top 50 genes in the most tumor-related module were identified, among which hub genes were selected and validated.

Results: Twenty co-expression modules were constructed on 13,865 genes from 62 lung tissues of COPD patients with or without LUAD, in which one module (blue) was most related to tumorigenesis. Functional enrichment analyses showed that the genes in the blue module were mainly enriched in cell cycle, DNA transcription/replication and cancer pathways, etc. Combined with protein-protein interaction network, MTA1, PKMYT1 and FZR1 genes had the most intramodular connectivity, which were regarded as the hub genes. However, only FZR1 was validated to be overexpressed in lung tissues of COPD with LUAD and cigarette smoke extract-stimulated A549 cells, a human LUAD cell line.

Conclusion: This study suggests overexpression of FZR1 may play a key role in the tumorigenesis of LUAD in patients with COPD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/OTT.S303544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187107PMC
June 2021

A case report of acute simultaneous cardiocerebral infarction: possible pathophysiology.

Ann Palliat Med 2021 May;10(5):5887-5890

Department of Emergency, Affiliated Yancheng Hospital, School of Medicine, Nanjing Medical University, Yancheng, China.

The occurrence of acute myocardial infarction (AMI) accompanied by ischemic cerebrovascular accidents has rarely been reported in previous articles. In this report, we present a 72-year-old female patient with massive cerebral infarction secondary to acute anterior and high lateral wall myocardial infarction, finally resulting in a deep coma. The patient ultimately failed to respond to aggressive resuscitation and succumbed to cardiogenic shock and fatal ventricular arrhythmias. We consider that the co-occurrence of these diseases is more than just a coincidence, and that there may be a connection between them. In this article, we performed an in-depth exploration and discussion of the explanation of this phenomenon. It is essential to recognize these situations in the early stages, which determines the follow-up treatment and prognosis. We suggest that decisions regarding patient management should be based on hemodynamic stability, close cardiac monitoring, and the site of cerebral infarction, and also emphasize that the evaluation of hemodynamic status in these patients is a prerequisite for deciding whether to treat the cerebral or coronary infarction first. The present report is written for the purpose of reminding readers of this rare and severe situation, and to emphasize the necessity for further research on how to deal with it best.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/apm-21-808DOI Listing
May 2021

Autophagy modulates FSS-induced epithelial-mesenchymal transition in hepatocellular carcinoma cells.

Mol Carcinog 2021 Jun 9. Epub 2021 Jun 9.

Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.

Hepatocellular carcinoma is a highly fatal disease and threatens human health seriously. Fluid shear stress (FSS), which is caused by the leakage of plasma from abnormally permeable tumor blood vessels and insufficient lymphatic drainage, has been identified as contributing pathologically to cancer metastasis. Autophagy and epithelial-mesenchymal transition (EMT) are both reported to be involved in cancer cell migration and invasion, but little has been revealed about the interaction between autophagy and EMT under a tumor mechanical microenvironment. Here, we identified that exposure to 1.4 dyne/cm FSS could promote the formation of autophagosomes and significantly increase the expressions of autophagy-related markers of beclin1 and ATG7, and the ratio of LC3Ⅱ/Ⅰ in both of HepG2 and QGY-7703 cells. The FSS loading also elevated the levels of mesenchymal markers N-cadherin, Vimentin, Twist, Snail, and β-catenin, while the epithelial markers E-cadherin showed a decrease. Once the autophagy was blocked by 3-methyladenine (3-MA) or knocking ATG5 down, the occurrence of FSS-induced EMT was inhibited dramatically according to the expression and translocation of E-cadherin, N-cadherin, and β-catenin. Given the effect of EMT on cell migration, we observed that inhibition of autophagy could impede FSS-induced cell migration. Collectively, this study demonstrated that autophagy played a crucial role in FSS-induced EMT and cell migration in hepatocellular carcinoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/mc.23327DOI Listing
June 2021

An integrated approach to uncover quality markers of stir-baking Semen Cuscuta with salt solution preventing recurrent spontaneous abortion based on chemical and metabolomic profiling.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jul 11;1177:122727. Epub 2021 May 11.

Key Laboratory of Chinese Medicinal Formula of Anhui Province, Anhui University of Chinese Medicine, Hefei 230031, PR China; Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology, Bozhou 236800, PR China; Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, PR China. Electronic address:

The previous research of clinical big data mining showed that stir-baking Semen Cuscuta with salt solution (YP) ranked the first in the usage rate of treating abortion caused by kidney deficiency. At the same time, pharmacodynamic studies also showed that YP has better effect on improving recurrent spontaneous abortion (RSA) compared to raw products of Semen Cuscuta (SP). However, there were few studies on the biomarkers of YP improving RSA. In this study, the chemical and metabonomic profiling were used to screen the quality markers of YP on improving RSA. Firstly, a metabolomics study was carried out to select representative biomarkers of RSA. The ultra-high performance liquid chromatography coupled with electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) technique was used to investigate the components of exogenous and endogenous in serum of rats after administrated with YP and SP. As a result, 14 differential compounds were identified between the serum of rats administrated SP and YP. Compared to SP, there was an upward trend in YP of the compounds including kaempferol-3-glucuronide, iso-kaempferol-3-glucuronide, (1S) -11-hydroxyhexadecanoic acid and 3-phenylpropionic acid. Meanwhile, there was a reducing trend in YP of the compounds including kaempferol 3-arabinofuranoside, apigenin-3-O-glucoside, hyperoside, caffeic acid-β-D glucoside, dicaffeoylquinic acid, linoleic acid, 3,4-dicaffeoylquinic acid, caffeic acid, palmitic acid and methyl myristate. 12 biomarkers for RSA indication were identified. SP and YP have a certain effect on the endogenous biomarker. The regulation effect of YP was higher than that of SP. The main metabolic pathways included phenylalanine, tyrosine and tryptophan biosynthesis, glycerophospholipid metabolism, fatty acid biosynthesis, sphingolipid metabolism, biosynthesis of unsaturated fatty acids. This study demonstrated a promising way to elucidate the active chemical and endogenous material basis of TCM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jchromb.2021.122727DOI Listing
July 2021

Lumbar Scheuermann's disease found in a patient with osteogenesis imperfecta (OI) caused by a heterozygous mutation in COL1A2 (c.4048G > A): a case report.

BMC Musculoskelet Disord 2021 Jun 7;22(1):525. Epub 2021 Jun 7.

Department of Endocrinology and Metabolism, Institute of Endocrinology, NHC Key Laboratory of Diagnosis and Treatment of Thyroid Diseases, The First Affiliated Hospital of China Medical University, Shenyang, P. R. China.

Background: Osteogenesis imperfecta (OI) is a heterogeneous connective tissue disorder characterized by increased bone fragility and a series of extraskeletal manifestations. Approximately 90 % of OI cases are caused by type I collagen variants encoded by the collagen type I alpha 1 (COL1A1) or type I alpha 2 (COL1A2) gene. Lumbar Scheuermann's disease is an atypical type of Scheuermann's disease accompanied by Schmorl's nodes and irregular endplates but without pronounced kyphosis. Although the etiology of Scheuermann's disease is unclear, genetic and environmental factors are likely.

Case Presentation: Here, we report a 32-year-old male patient who experienced multiple brittle fractures. Gene sequencing revealed a heterozygous mutation, c.4048G > A (p.G1350S), in the COL1A2 gene, and the patient was diagnosed with OI. Magnetic resonance imaging of his thoracolumbar spine revealed multiple Schmorl's nodes.

Conclusions: This is the first reported case of OI coexisting with the spinal presentation of Scheuermann's disease. It is speculated that the COL1A2 gene mutation might be an underlying novel genetic cause of Scheuermann's disease. In conclusion, this case demonstrates the relationship between Scheuermann's disease and OI for the first time and enriches the genotype-phenotype spectrum of OI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-021-04401-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185920PMC
June 2021

Effects of mixed meal tolerance test on gastric emptying, glucose and lipid homeostasis in obese nonhuman primates.

Sci Rep 2021 Jun 4;11(1):11866. Epub 2021 Jun 4.

Cardiovascular and Metabolic Diseases, Crown Bioscience, Inc., 6 Beijing West Road, Taicang, Jiangsu Province, 215400, People's Republic of China.

Meal ingestion elicits a variety of neuronal, physiological and hormonal responses that differ in healthy, obese or diabetic individuals. The mixed meal tolerance test (MMTT) is a well-established method to evaluate pancreatic β-cell reserve and glucose homeostasis in both preclinical and clinical research in response to calorically defined meal. Nonhuman primates (NHPs) are highly valuable for diabetic research as they can naturally develop type 2 diabetes mellitus (T2DM) in a way similar to the onset and progression of human T2DM. The purpose of this study was to investigate the reproducibility and effects of a MMTT containing acetaminophen on plasma glucose, insulin, C-peptide, incretin hormones, lipids, acetaminophen appearance (a surrogate marker for gastric emptying) in 16 conscious obese cynomolgus monkeys (Macaca fascicularis). Plasma insulin, C-peptide, TG, aGLP-1, tGIP, PYY and acetaminophen significantly increased after meal/acetaminophen administration. A subsequent study in 6 animals showed that the changes of plasma glucose, insulin, C-peptide, lipids and acetaminophen were reproducible. There were no significant differences in responses to the MMTT among the obese NHPs with (n = 11) or without (n = 5) hyperglycemia. Our results demonstrate that mixed meal administration induces significant secretion of several incretins which are critical for maintaining glucose homeostasis. In addition, the responses to the MMTTs are reproducible in NHPs, which is important when the MMTT is used for evaluating post-meal glucose homeostasis in research.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-021-91027-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178340PMC
June 2021

Efficacy and safety of Kanglaite injection combined with chemotherapy for women breast cancer: A protocol for systematic review and meta-analysis of randomized clinical trials.

Medicine (Baltimore) 2021 Jun;100(22):e26245

Anhui Medical University, School of Public Health.

Background: Breast cancer was the second cause of cancer death and approximately accounted for 30% of all newly diagnosed cancer in American women. Adjuvant chemotherapy is the preferred treatment approach for breast patients. Kanglaite injection (KI) was commonly used as adjuvant chemotherapy combined with chemotherapy for women breast cancer which could increase chemotherapy efficacy and alleviate chemotherapy drugs induced adverse events, however, the efficacy and safety for KI combined western medicine remains controversial. Thus, we conducted this protocol of systematic review and meta-analysis to estimate the efficacy and safety of KI combined with western medicine for women breast cancer.

Methods: This study will search electronic database included English medicals databases and Chinese databased up to May 2021. The main outcomes of this study include clinical efficacy rate. Adverse reaction rate, Karnofsky Performance Status and immune function were defined as the secondary outcomes.

Results: This protocol study will comprehensively evaluate the efficacy and safety of KI combined with chemotherapy for women breast cancer.

Conclusion: This protocol for systematic review and meta-analysis will evaluate the efficacy and safety of KI combined with chemotherapy for women breast cancer, aiming to provide optimal therapy for women breast cancer patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000026245DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183730PMC
June 2021

Systematic identification of the cancer pathways and molecules related with breast cancer immunogenicity.

Med Oncol 2021 Jun 4;38(7):79. Epub 2021 Jun 4.

Department of Medicine, Jiangsu Cancer Hospital and Jiangsu Institution of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, 210009, People's Republic of China.

To identify molecular features related to immunogenic activity in breast cancer (BC) and provide new targets and directions for BC immunotherapy, we firstly used ESTIMATE to evaluate the degree of immune cell infiltration of the BC patients in TCGA and METABRIC, and explore the relationship between the degree of immune cell infiltration and prognosis of BC patients. Then, we identified the cancer pathways, proteins and miRNAs related to BC immunogenicity, predicted the target genes of these miRNAs, and identified the pathways related to these target genes with KEGG pathway enrichment analysis. We also explored the correlation between PD-L1 expression level and cancer pathways and found that PD-L1 expression showed a positive association with cancer-related pathways. In this article we have successfully identified several cancer-related pathways, proteins, miRNAs, and their target genes, which provided promising new targets for BC immunotherapy. And PD-L1 blockade therapy may be more effective in BC patients with the activation of some cancer-related pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12032-021-01515-9DOI Listing
June 2021

Improving Ammonia Detecting Performance of Polyaniline Decorated rGO Composite Membrane with GO Doping.

Materials (Basel) 2021 May 25;14(11). Epub 2021 May 25.

School of Microelectronics, School of Electronics and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Gas-sensing performance of graphene-based material has been investigated widely in recent years. Polyaniline (PANI) has been reported as an effective method to improve ammonia gas sensors' response. A gas sensor based on a composite of rGO film and protic acid doped polyaniline (PA-PANI) with GO doping is reported in this work. GO mainly provides NH adsorption sites, and PA-PANI is responsible for charge transfer during the gas-sensing response process. The experimental results indicate that the NH gas response of rGO is enhanced significantly by decorating with PA-PANI. Moreover, a small amount of GO mixed with PA-PANI is beneficial to increase the gas response, which showed an improvement of 262.5% at 25 ppm comparing to no GO mixing in PA-PANI.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14112829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198450PMC
May 2021

Improvement of the Adhesion and Diamond Content of Electrodeposited Cu/Microdiamond Composite Coatings by a Plated Cu Interlayer.

Materials (Basel) 2021 May 15;14(10). Epub 2021 May 15.

Department of Marine Engineering, National Taiwan Ocean University, Keelung 20224, Taiwan.

Diamond-incorporated copper metal matrix layers were fabricated on brass substrates by using electrodeposition technology in this study. To improve the adhesion of the composite coatings on the brass substrate, a plated copper was applied as the interlayer between the multilayers and the substrate. The surface morphologies of the interlayer and the diamond-incorporated copper composite layers were studied by scanning electron microscopy. The effect of the copper interlayer on the incorporation and the distribution of the diamond content in the coatings was analyzed by surface roughness, electrochemical impedance spectroscopy, and cyclic voltammetry. The diamond content of the composite coating was measured by energy-dispersive X-ray. The film thickness was evaluated by the cross-sectional technique of focused ion beam microscopy. The element, composition, and crystallization direction of diamond with Cu matrix was measured by X-ray diffraction and transmission electron microscope. The adhesion of the multilayers was studied by scratch tests. The experiment results indicated that the diamond content and distribution of the coating were higher and more uniform with the Cu interlayer than that without one. The plated copper interlayer reduced the electrical double-layer impedance and enhanced the adsorption of diamond particles by the surrounding Cu ions, which promoted the diamond content in the composite coatings. The roughened surface caused by the plated Cu interlayer also improved the substrate's mechanical interlock with the composite coating, which contributed to the strong adhesion between them.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma14102571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156921PMC
May 2021

Telmisartan Mitigates TNF-α-Induced Type II Collagen Reduction by Upregulating SOX-9.

ACS Omega 2021 May 22;6(17):11756-11761. Epub 2021 Apr 22.

Department of Rheumatology and Immunology, Zibo Central Hospital, Zibo 255036, China.

The proinflammatory cytokine tumor necrosis factor-α (TNF-α)-induced degradation of extracellular matrix (ECM), such as type II collagen in chondrocytes, plays an important role in the development of osteoarthritis (OA). Telmisartan, an angiotensin II (Ang-II) receptor blocker, is a licensed drug used for the treatment of hypertension. However, the effects of Telmisartan in tumor necrosis factor-α (TNF-α)-induced damage to chondrocytes and the progression of OA are unknown. In this study, we found that treatment with Telmisartan attenuated TNF-α-induced oxidative stress by reducing the levels of mitochondrial reactive oxygen species (ROS) and the production of protein carbonyl in human C28/I2 chondrocytes. Interestingly, Telmisartan inhibited TNF-α-induced expression and secretions of proinflammatory mediators such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and monocyte chemotactic protein 1 (MCP-1). Notably, stimulation with TNF-α reduced the levels of type II collagen at both the mRNA and the protein levels, which was rescued by the treatment with Telmisartan. Mechanistically, we found that Telmisartan restored TNF-α-induced reduction of SOX-9. Silencing of SOX-9 blocked the inhibitory effects of Telmisartan against TNF-α-induced degradation of type II collagen. These findings suggest that Telmisartan might be a potential and promising agent for the treatment of OA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.1c01170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154015PMC
May 2021

Isolation, Identification, and Anti-Inflammatory Activity of Polysaccharides of .

Biomacromolecules 2021 06 23;22(6):2451-2459. Epub 2021 May 23.

Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, College of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China.

The present study aimed to purify, structurally characterize, and evaluate the anti-inflammatory activity of the polysaccharide extracted from . Two purified polysaccharides (PTA-1 and PTA-2) were obtained via DEAE-52 cellulose chromatography. Their structural characterizations and antioxidant activity were in vitro analyzed. To evaluate the anti-inflammatory activity of PTA-2, the levels of inflammatory cytokines, intracellular ROS production, and the inhibitory effects of the transcriptional activation of the nuclear factor kappa B (NF-κB) signaling pathway were determined. PTA-1 comprises glucose (100%) with α-(1 → 3) glycosidic bonds, and PTA-2 comprises glucose (66.7%) and rhamnose (33.3%) formed by β-(1 → 3) glycosidic bonds. PTA-1 and PTA-2 showed strong antioxidant activity in vitro. Moreover, PTA-2 intervention (50, 100, and 200 μg/mL) suppressed the production of inflammatory cytokines, the activation of NF-κB signaling, and reactive oxygen species production significantly. The results identified PTA-2 as a natural product that could be applied in anti-inflammatory drugs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.biomac.1c00235DOI Listing
June 2021

Determinants of exclusive breastfeeding for the first six months in China: a cross-sectional study.

Int Breastfeed J 2021 May 17;16(1):40. Epub 2021 May 17.

Department of Maternal and Child Health, Peking University School of Public Health, Beijing, China.

Background: Breast milk is the best source of essential nutrients and bioactive components for infants under 6 months. However, little is known about what affects breastfeeding intentions and practices of Chinese mothers. With measures of individual, setting, and sociocultural factors, this study examined determinants of exclusive breastfeeding in the first 6 months of infancy in China.

Methods: Data were obtained from a national cross-sectional survey in China in 2018 that included 5237 infants under 6 months with available measurements of breastfeeding. A 24-h reported food recall method was applied to assess breastfeeding and complementary food intake in the past 24 h. Potential breastfeeding determinants categorized into six aspects were measured: (1) infant health, (2) maternal sociodemographic characteristics, (3) maternal health, (4) breastfeeding support from family, friends, and workplace, (5) social support for breastfeeding, and (6) maternal breastfeeding experiences and knowledge. Reasons for non-commencement or early cessation of breastfeeding were evaluated for non-breastfed infants. For breastfed infants, multivariate logistic regression was used to explore the determinants of exclusive breastfeeding.

Results: About 30 % (29.5%) of infants under 6 months were exclusively breastfed; 2.3% (2.3%) had never been breastfed and 3.2% had ceased breastfeeding. No breast milk (60.7%), maternal illness (13.9%), and infant illness (13.1%) were the top three reasons for non-commencement of breastfeeding. Insufficient breast milk was the reason given for ceasing breastfeeding early by almost two thirds of caregivers who had stopped breastfeeding. The following factors were associated with exclusive breastfeeding: maternal higher education, formal employment with ≥6 months of paid maternity leave, support of the husband and best friends for breastfeeding, a breastfeeding-supportive society, and better breastfeeding knowledge and experiences (a previous successful breastfeeding experience ≥6 months and early initiation of breastfeeding). Maternal age of ≥40 years, caesarean delivery, and infant disease history were associated with non-exclusive breastfeeding.

Conclusions: The exclusive breastfeeding rate is still very low in China. Multidimensional barriers contribute to this situation. A comprehensive intervention framework is needed to increase optimal breastfeeding and achieve substantial public health gains.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13006-021-00388-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130252PMC
May 2021
-->