Publications by authors named "Xiaoli Luo"

53 Publications

Clinical value of NT-proBNP measurements in assessing patients in the pediatric intensive care unit.

Transl Pediatr 2021 May;10(5):1355-1360

Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Background: N-terminal brain natriuretic peptide precursor (NT-proBNP) and brain natriuretic peptide (BNP) are mainly produced and secreted in the heart. In 2008, the European Heart Association recommended that serum BNP/NT-proBNP levels should be included in one of the diagnostic criteria of heart failure. Serum NT-proBNP is more stable than BNP, and the detection results are less affected by objective factors, so it is widely used. At present, NT-proBNP has long been beyond the scope of heart failure markers, and has a wide range of clinical value in the evaluation and prediction of some serious diseases. This study prospectively studied the predictive value of serum NT-proBNP in pediatric intensive care unit (PICU).

Methods: This was a prospective study involving 375 children in the PICU. The patients were divided into three groups: non-risk, low-risk, and high-risk groups. Serum NT-proBNP levels and the 28-day mortality rate were analyzed.

Results: The serum NT-proBNP levels and the mortality of the high-risk group was significantly higher than those of the low- and non-risk groups (P<0.01 in both cases). Receiver operating characteristic curve (ROC curve) analysis showed that the area under the curve was 0.705 (P<0.001, sensitivity =0.643, specificity =0.692). Death multivariate binary logistic regression analysis indicated that NT-proBNP was not an independent factor for 28-day mortality.

Conclusions: Serum NT-proBNP was significantly correlated with the severity of illness for critically ill patients in PICU. Although high levels of NT-proBNP indicated greater severity, this was not an independent risk factor affecting the prognosis of patients.
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http://dx.doi.org/10.21037/tp-21-123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192984PMC
May 2021

Mediation effects of different sulfur forms on solubility, uptake and accumulation of Cd in soil-paddy rice system induced by organic carbon and liming.

Environ Pollut 2021 Jun 12;279:116862. Epub 2021 Mar 12.

School of Environmental Science and Engineering, Guangdong Provincial Key Lab for Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Laboratory for Lingnan Modern Agriculture, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China. Electronic address:

Liming is a safe and effective remediation practice for Cd contaminated acid paddy soil. The fate of Cd can also be strongly influenced by redox chemistry of sulfur. But it is unclear if, to what extent and how the combination of liming and sulfur mediation could further control Cd uptake by paddy rice. A rice cultivation pot experiment was conducted to evaluate the impact of different sulfur forms (S and SO in KSO) on the solubility, uptake and accumulation of Cd in the soil-paddy rice system and how liming and reducing organic carbon mediate the process. Results showed that under neutral soil circumstances achieved by liming, co-application of KSO and glucose significantly reduced brown rice Cd by 33%, compared to liming alone. They made it more readily for Cd to be precipitated into CdS/CdS or co-precipitate with newly formed FeS/FeS/iron oxides. The higher pH balancing capability of KSO as well as liming kept the newly formed sulfide or iron containing minerals negatively charged to be more prone to adsorb Cd, that kept the porewater Cd the lowest among all the treatments. Individual KSO showed significant promoting effect on soil Cd solubility due to SO chelation effect. Furthermore, KSO had much weaker inhibiting effect on Cd translocation from root to grain, it showed no significant attenuating effect on brown rice Cd. S containing treatments displayed weaker or no attenuating effect on brown rice Cd due to its strong soil acidification effect. On the basis of liming, organic carbon induced sulfur (KSO) mediation showed great application potential for safe production on large areas of acid paddy soil contaminated by Cd.
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http://dx.doi.org/10.1016/j.envpol.2021.116862DOI Listing
June 2021

Molecular characterization of long-term survivors of hepatocellular carcinoma.

Aging (Albany NY) 2021 03 3;13(5):7517-7537. Epub 2021 Mar 3.

Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.

Hepatocellular carcinoma is one of the most fatal cancers, and the majority of patients die within three years. However, a small proportion of patients overcome this fatal disease and survive for more than five years. To determine the molecular characteristics of long-term survivors (survival ≥ 5 years), we analyzed the genomic and clinical data of hepatocellular carcinoma patients from The Cancer Genome Atlas and the International Cancer Genome Consortium databases, and identified molecular features that were strongly associated with the patients' prognosis. Genes involved in the cell cycle were expressed at lower levels in tumor tissues from long-term survivors than those from short-term survivors (survival ≤ 1 years). High levels of positive regulators of the G/S cell cycle transition (cyclin-dependent kinase 2 [], , Cyclin E2 [], , ) were potential markers of poor prognosis. Hepatocellular carcinoma patients with mutations were mainly belonged to the short-term survivor group. Abemaciclib, an FDA-approved selective inhibitor of CDK4/6, inhibited the cell proliferation and tumor growth of hepatocellular carcinoma cells and . Thus, high G/S transition-related gene levels and mutations are promising diagnostic biomarkers for short-term survivals, and abemaciclib may be a potential targeted drug for hepatocellular carcinoma.
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http://dx.doi.org/10.18632/aging.202615DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7993728PMC
March 2021

Chylous ascites following repair of total anomalous pulmonary venous connection coexisting with a persistent left superior vena cava in a neonate: a case report.

Transl Pediatr 2021 Jan;10(1):188-193

Department of Pediatric Critical Medicine, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Chylous ascites refers to the accumulation of lymphatic fluid in the peritoneal cavity. The causes of chylous ascites are various, and commonly include traumatic injury and obstruction, which disrupt the lymphatic system. In addition, cardiothoracic surgery may injure the thoracic duct and lead to chylothorax. However, there are very few reported cases of isolated chylous ascites developing following cardiothoracic surgery. In this paper, we report a case of postoperative chylous ascites in a full-term neonate. The infant underwent cardiothoracic surgery via thoracotomy to repair total anomalous pulmonary venous connection coexisting with a persistent left superior vena cava on day of life 17, and there was a significant increase in abdominal girth on postoperative day 12 (day of life 29). Abdominal ultrasound revealed an 8 mm thick ascites without pleural effusion. Abdominal paracentesis was performed and the milky-white peritoneal fluid was positive for Sudan III staining and the chylous test. The triglyceride concentration of the ascitic fluid was 691 mg/dL and the concentration of protein was 39.4 g/L. Additionally, the ascitic fluid also contained 6 360×10/L of white blood cells, predominantly lymphocytes. These results suggested the infant developed chylous ascites. Conservative management with fasting and medium-chain triglycerides-based formula successfully resolved the chylous ascites without reoccurrence. We present our experience of this rare condition and discuss the possible causes of chylous ascites in this case.
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http://dx.doi.org/10.21037/tp-20-258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882300PMC
January 2021

Strategic management of pediatric intensive care unit in a tertiary children's hospital in southwest China during the COVID-19 pandemic.

Transl Pediatr 2020 Dec;9(6):849-862

Department of Pediatric Critical Medicine, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Since early December 2019, patients with unknown pneumonia have been found in Wuhan City, Hubei Province, China. The pathogen in these cases was quickly identified as a new type of coronavirus. The World Health Organization named it 2019 novel coronavirus (2019-nCoV), and the disease caused by the pathogen was called 2019 Coronavirus Disease (COVID-19), which was characterized by higher pathogenicity, transmission of human to human. So it has rapidly spread to more than 190 countries all over the world. With the sudden outbreak of COVID-19, preventing the spread of COVID-19 is the primary problem. Despite fewer children than adults have been affected by the COVID-19, Pediatric Intensive Care Unit (PICU), as a ward for critically ill patients, is also confronted with high risk of 2019-nCoV infection. It is necessary for PICU managers to thoroughly carry out scientific and effective department management and carefully execute of infection control measures to prevent the transmission. According to recommendations for the COVID-19 prevention and control, the relevant guidelines and the authors' work experience, this paper proposes and optimizes the strategic plan for the management of COVID-19 outbreak in PICU, and emphasizes that department managers should conduct comprehensive risk assessments, manage the pediatric patients and healthcare workers meticulously, strengthen the implementation of infection control measures, and use risk management and process control to effectively manage the department as well as to protect the safety of both the patients and the staff.
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http://dx.doi.org/10.21037/tp-20-422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7804477PMC
December 2020

Rational design of metal-organic frameworks to deliver methotrexate for targeted rheumatoid arthritis therapy.

J Control Release 2021 02 15;330:119-131. Epub 2020 Dec 15.

Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan 410013, China; Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Biological Nanotechnology of National Health Commission, Changsha, Hunan 410008, China. Electronic address:

Methotrexate (MTX) has been used as an anchor drug for the treatment of rheumatoid arthritis (RA), while the patients with chronic MTX administration suffer from severe side-effects. To this end, targeted delivery of MTX by nanomedicine has attracted great interest. In this work, we aimed to employ metal-organic frameworks (MOFs) as nanocarrier to deliver MTX by virtue of its facile and green preparation and exceptionally high drug loading. While MTX could be easily and effectively loaded via different MOF construction strategies, such as direct coordination, physical encapsulation, and covalent conjugation, we found that most of the MTX loading MOFs showed premature and burst drug release, attributable to the unstable coordination between MTX and metals. To address this issue, we rationally designed the MOFs by conjugating MTX with tannic acid (TA) at 2:1 M ratio and then coordinating with ferric ion (Fe), followed by surface modification of hyaluronic acid (HA). The resulting MOFs achieved ultra-high drug loading (45%) and sustained drug release, and could selectively recognize the diseased cells for anti-inflammatory effect. The in vivo therapeutic evaluation suggested that the MOFs could enhance the anti-rheumatic activity of MTX while minimizing its toxic effects by targeted drug delivery, resulting in improved therapeutic index. This work provides a biocompatible nano-platform to deliver MTX for RA treatment, and importantly, calls for special attention to the gap between MOFs design and their biological applications, and the gap needs to be filled by careful evaluation of in vivo stability and burst drug release.
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http://dx.doi.org/10.1016/j.jconrel.2020.10.069DOI Listing
February 2021

Characterization, mechanism of cypermethrin biosorption by Saccharomyces cerevisiae strains YS81 and HP and removal of cypermethrin from apple and cucumber juices by inactive cells.

J Hazard Mater 2021 04 27;407:124350. Epub 2020 Oct 27.

College of Food Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, People's Republic of China; Institute of Food Processing and Safety, Sichuan Agricultural University, Ya'an, Sichuan 625014, People's Republic of China. Electronic address:

Cypermethrin is a common food contaminant and environmental pollutant that cause health threats to animals and humans. In this study, the characterization, mechanism, and application of cypermethrin removal by Saccharomyces cerevisiae were investigated. The binding of cypermethrin by the strains S. cerevisiae YS81 and HP was rapid and reached equilibrium at 2-8 h. The removal efficiency was dependent on incubation temperature and yeast concentration, whereas cypermethrin binding was not affected by pH. Heat and acid treatments enhanced the binding ability. Both strains survived in simulated digestion juices and removed cypermethrin effectively under simulated gastrointestinal conditions. Among the strains tested, the YS81 strain was the better candidate for cypermethrin concentration reduction. For the two S. cerevisiae strains, the biosorption kinetics and isotherm followed the pseudo-second-order model and Langmuir model well. The cell walls and the protoplasts were the main yeast cell components involved in cypermethrin binding. Fourier transformed infrared spectroscopy analysis revealed that -OH, -NH, -C-N, -COO, and -C-O played a major role in binding cypermethrin. Inactive cells effectively removed cypermethrin from apple and cucumber juices and did not affect the physico-chemical properties. Therefore, S. cerevisiae strains YS81 and HP may be used for cypermethrin reduction in food or feed.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124350DOI Listing
April 2021

Exposure to maternal diabetes induces endothelial dysfunction and hypertension in adult male rat offspring.

Microvasc Res 2021 01 18;133:104076. Epub 2020 Sep 18.

Department of Cardiology, Fujian Heart Center, Provincial Institute of Coronary Disease, Fujian Medical University Union Hospital, Fuzhou, Fujian, China. Electronic address:

The adverse environment in early life can modulate adult phenotype, including blood pressure. Our previous study shows, in a rat streptozotocin (STZ)-induced maternal diabetes model, fetal exposure to maternal diabetes is characterized by established hypertension in the offspring. However, the exact mechanisms are not known. Our present study found, as compared with male control mother offspring (CMO), male diabetic mother offspring (DMO) had higher blood pressure with arterial dysfunction, i.e., decreased acetylcholine (Ach)-induced vasodilation. But there is no difference in blood pressure between female CMO and DMO. The decreased Ach-induced vasodilation was related to decreased nitric oxide (NO) production in the endothelium, not NO sensitivity in vascular smooth muscle because sodium nitroprusside (SNP)-mediated vasodilation was preserved; there was decreased NO production and lower eNOS phosphorylation in male DMO. The reactive oxygen species (ROS) level was increased in male DMO than CMO; normalized ROS levels with tempol increased NO production, normalized Ach-mediated vasodilation, and lowered blood pressure in male DMO rats. It indicates that diabetic programming hypertension is related to arterial dysfunction; normalizing ROS might be a potential strategy for the prevention of hypertension in the offspring.
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http://dx.doi.org/10.1016/j.mvr.2020.104076DOI Listing
January 2021

An efficient imidation of thioethers with nitrene in water.

Org Biomol Chem 2020 Aug;18(33):6497-6501

Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China.

The first imidation of thioethers with free nitrene in water was realized. N-Cbz sulfilimines are formed via imidation of thioethers with free nitrene generated from α elimination of nosyloxycarbamates. In this work, water is successfully applied as solvent for free nitrene, and transition metal catalyst is not needed.
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http://dx.doi.org/10.1039/d0ob01539cDOI Listing
August 2020

Author Correction: The Genome Sequences of 90 Mushrooms.

Sci Rep 2020 May 18;10(1):8460. Epub 2020 May 18.

College of Biological Big Data, Yunnan Agriculture University, Kunming, 650201, Yunnan, China.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41598-020-63941-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235220PMC
May 2020

Upregulation of TIGIT and PD-1 in Colorectal Cancer with Mismatch-repair Deficiency.

Immunol Invest 2021 May 13;50(4):338-355. Epub 2020 May 13.

Department of Anal-Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan, China.

: The role of T cell Ig and ITIM domain (TIGIT) and programmed cell death-1 (PD-1) in colorectal cancer (CRC) with mismatch repair deficiency is unknown.: This was a study of 60 CRC patients with mismatch repair deficiency and 30 healthy controls between June 2015 and October 2015.: The expression of Foxp3, PD-1, and TIGIT was higher in cancer tissues compared with adjacent mucosa (all < .05). Patients with advanced TNM stage had a significantly higher expression of TIGIT ( = .025) and PD-1 ( = .020) than patients with early-stage CRC. The disease-free survival (DFS) of patients with high TIGIT (HR = 3.96, 95%CI: 1.34-11.69, = .013) or PD-1 (HR = 214.8, 95%CI: 49.88-925.2, < .001) expression were better. The overall survival (OS) of the patients with CRC and high expression of PD-1 was worse than those with low expression (HR = 4.01, 95%CI:1.26-12.69, = .019).: TIGIT and PD-1 are upregulated in CRC with mismatch repair deficiency and associated with TNM stage and DFS.
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http://dx.doi.org/10.1080/08820139.2020.1758130DOI Listing
May 2021

Case report of holocarboxylase synthetase deficiency (late-onset) in 2 Chinese patients.

Medicine (Baltimore) 2020 May;99(18):e19964

Department of Pediatrics Intensive Care Unit, Chengdu Women's and Children's Central Hosptial, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People's Republic of China.

Rationale: Holocarboxylase synthetase (HCLS) deficiency, especially the late-onset type, is a rare disease. Affected patients can present with irreversible metabolic acidosis and may be misdiagnosed with a glucose metabolic disorder. Prompt and correct diagnosis and treatment can reduce mortality to a great extent.

Patient Concerns: We report 2 Chinese patients who were diagnosed with late-onset HCLS deficiency. The age of onset of the 2 patients was approximately 8 months. The 2 patients had skin lesions, severe profound metabolic acidosis, dyspnea, and hyperglycemia.

Diagnoses: The results of urinary and blood organic acid analysis with gas chromatography/mass spectrometry revealed multiple carboxylase deficiency. Maple syrup urine disease and diabetic ketoacidosis could not be excluded. This finding is different from those of hypoglycemic complications reported in previous reports. Human genetic analysis eventually provided a definite diagnosis.

Interventions: Prompt oral treatment with biotin dramatically corrected the metabolic imbalances of the 2 patients, and continued oral biotin therapy was essential to the improvement of their prognoses.

Outcomes: Their metabolic disorders were corrected within 48 hours. During long-term follow-up, the patients achieved developmental milestones.

Lessons: Late-onset HCLS deficiency may present with obvious hyperglycemia. Human genetic analysis eventually provided a definite diagnosis. Prompt treatment with biotin is vital to correct metabolic imbalances, and continued therapy is essential to the improving long-term prognoses. Their mutations were p.R508W and c.1088T > A, and these mutations might represent hot-spot genes in Chinese populations with HCLS deficiency. The variants c.1484T > G(p.L495*) and c.835G > T(p.E279x) are likely pathogenic, and more studies are needed to confirm these results.
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http://dx.doi.org/10.1097/MD.0000000000019964DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7440356PMC
May 2020

Ligands dissociation induced gold nanoparticles aggregation for colorimetric Al detection.

Anal Chim Acta 2019 Dec 26;1087:76-85. Epub 2019 Aug 26.

Xiangya School of Pharmaceutical Sciences, State Key Laboratory of Powder Metallurgy, Central South University, Changsha, Hunan, 410013, China. Electronic address:

Aluminum is a very important analyte, and developing biosensors for aluminum is an important analytical task. In this work, we report a novel mechanism to design colorimetric sensor based on gold nanoparticles (AuNPs). The AuNPs were prepared by reducing HAuCl using catechols, and the resulting AuNPs can be directly adapted for Al detection without any post-modifications, showing high sensitivity and selectivity against other metal ions. Interestingly, our mechanistic studies revealed that Al-induced AuNPs aggregation was not due to the formation of interparticle crosslinks that refers to the design principle of most AuNPs-based colorimetric sensors reported before. But rather, Al competitively coordinated with the capping ligands on AuNPs surface through the formation of stable Al-O bond, which dissociated these ligands from AuNPs surface. As a result, the AuNPs aggregated due to the loss of surface stabilizers. Based on this mechanism, several catechols, including pyrocatechol (PC), 3-(3,4-dihydroxyphenyl) propionic acid (DHCA), levodopa (LDA) and dopamine (DA), were used as reductant to prepare AuNPs for Al sensing, and the AuNPs prepared by DA (AuNPs/DA) displayed the highest sensitivity, with detection limit of 0.81 μM. The sensor was then tested for Al content analysis in river water and food samples, and the results supported its practical applications. Importantly, this work expands the design principles for colorimetric sensors by using AuNPs.
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http://dx.doi.org/10.1016/j.aca.2019.08.045DOI Listing
December 2019

AFAP1-AS1 Promotes Proliferation of Pituitary Adenoma Cells through miR-103a-3p to Activate PI3K/AKT Signaling Pathway.

World Neurosurg 2019 Oct 9;130:e888-e898. Epub 2019 Jul 9.

Department of Neurosurgery, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

Background: We previously found that AFAP1-AS1 regulates the cell growth of pituitary tumor cells; however, the mechanism still remains unclear. Here, we investigated whether AFAP1-AS1 acts as a competing endogenous RNA of miR-103a-3p to regulate pituitary adenoma growth via the PI3K/AKT pathway.

Methods: The bind between AFAP1-AS1 and rno-miR-103a-3p was measured by luciferase reporter assay, and rno-miR-103a-3p expression was measured by quantitative reverse transcription polymerase chain reaction. Proliferation, cell cycle, and apoptosis were measured by cell counting kit 8 and flow cytometry. Rat growth hormone (GH) and prolactin (PRL) levels in culture supernatant of GH3 and MMQ cells were measured by enzyme-linked immunosorbent assay.

Results: AFAP1-AS1 binds to rno-miR-103a-3p in rat pituitary adenoma cells. Additionally, rno-miR-103a-3p overexpression suppressed rat pituitary adenoma cell proliferation, induced cell apoptosis, arrested cell cycle in the G/S phase, reduced GH and PLR secretion, and inhibited the PI3K/AKT signaling pathway. Activated PI3K/AKT signaling pathway revised the effect of rno-miR-103a-3p overexpression on proliferation and GH and PLR secretion. Coexpression of both si-AFAP1-AS1 and rno-miR-103a-3p inhibitor promoted cell proliferation and cell cycle progression, reduced cell apoptosis, enhanced GH and PLR secretion, and activated the PI3K/AKT signaling pathway in rat pituitary adenoma cells.

Conclusion: We found that AFAP1-AS1 and miR-103a-3p could be a potential therapeutic target for pituitary adenoma.
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http://dx.doi.org/10.1016/j.wneu.2019.07.032DOI Listing
October 2019

Cotton plant defence against a fungal pathogen is enhanced by expanding BLADE-ON-PETIOLE1 expression beyond lateral-organ boundaries.

Commun Biol 2019 21;2:238. Epub 2019 Jun 21.

1The State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China.

In the plant response to pathogen infection, many genes' expression is temporally induced, while few spatially induced expression genes have been reported. Here, we show that GhBOP1 can autonomously expand expression from restrained tissue when plants are attacked by , which is considered to be spatially induced expression. Loss- and gain-of-function analyses show that GhBOP1 is a positive regulator in the modulation of plant resistance to . Yeast two-hybrid assays, luciferase complementation imaging and GUS reporting show that GhBOP1 interaction with GhTGA3 promotes its activation activity, regulating the expression of down-stream defence-related genes. Moreover, the induced spatial expression of GhBOP1 is accompanied by GhBP1 repression. Both antagonistically regulate the lignin biosynthesis, conferring cotton plants enhanced resistance to . Taken together, these results demonstrate that GhBOP1 is an economic positive regulator participating in plant defence through both the GhBOP1-GhTGA3 module and lignin accumulation.
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http://dx.doi.org/10.1038/s42003-019-0468-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6588604PMC
April 2020

Epidemiology of Pediatric Severe Sepsis in Main PICU Centers in Southwest China.

Pediatr Crit Care Med 2019 12;20(12):1118-1125

Department of Pediatric Critical Care, Chongqing Qijiang People's Hospital, Qijiang, Chongqing, China.

Objectives: To estimate the prevalence, management, and outcomes of pediatric severe sepsis in the main PICUs in Southwest China.

Design: A prospective, observational, and multicenter study.

Setting: Eight PICUs in Southwest China with 19 (13-24) beds and 1,322 (1,066-1,452) annual admissions each.

Patients: A total of 10,598 patients (29 d to 18 yr old) were consecutively admitted between September 1, 2016, and August 31, 2017. All patients were screened and evaluated for severe sepsis or septic shock. Of them, 10,353 patients were excluded due to incomplete data or not meeting the consensus criteria for severe sepsis or septic shock; 245 patients were included with complete data.

Interventions: None.

Measurements And Main Results: Finally, 245 patients who were diagnosed with severe sepsis or septic shock were included in the study, with an incidence rate of 2.3%. Of them, 64.0% of the enrolled patients were male with 80.8% being less than 5 years old and 60.8% being from rural areas. The respiratory system was the most common organ system in which dysfunction was observed (76.7%) as well as the most frequently infected site (37.6%). The primary therapies were antibiotics (99.0%), immunoglobulin (88.3%), mechanical ventilation (78.4%), vasoactive infusions (59.6%), and corticosteroids (46.1%). Among the 188 patients who had respiratory dysfunction, 173(92%) required mechanical ventilation and 39 (20.7%) met the criteria for pediatric acute respiratory distress syndrome. Seven of the patients with pediatric acute respiratory distress syndrome died (7/39, 17.9%). The median durations for mechanical ventilation and vasoactive medications were 123.5 hours (35.25-226.00 hr) and 2 days (1-5 d), respectively. Eighty-six percent of patients had multiple organ dysfunction syndrome at the point at which severe sepsis was recognized, and 31% had underlying conditions. The hospital mortality rate was 18.8%.

Conclusions: This report is the first to present the prevalence, treatment, and outcomes of pediatric severe sepsis in the main PICU centers in Southwest China. The mortality rate remains high; therefore, improved clinical management and implementation of large-scale clinical trials are necessary to improve early diagnoses and treatment.
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http://dx.doi.org/10.1097/PCC.0000000000002079DOI Listing
December 2019

Impact of Healthcare-Associated Infections on Length of Stay: A Study in 68 Hospitals in China.

Biomed Res Int 2019 18;2019:2590563. Epub 2019 Apr 18.

Peking University People's Hospital, Beijing 100044, China.

Healthcare-associated infections (HAIs) not only bring additional medical cost to the patients but also prolong the length of stay (LOS). 2119 HAI case-patients and 2119 matched control-patients were identified in 68 hospitals in 14 primary sampling provinces of 7 major regions of China. The HAI caused an increase in stay of 10.4 days. The LOS due to HAI increased from 9.7 to 10.9 days in different levels of hospitals. There was no statistically significant difference in the increased LOS between different hospital levels. The increased LOS due to HAI in different regions was 8.2 to 12.6 days. Comparing between regions, we found that the increased LOS due to HAI in South China is longer than other regions except the Northeast. The gastrointestinal infection (GI) caused the shortest extra LOS of 6.7 days while the BSI caused the longest extra LOS of 12.8 days. The increased LOS for GI was significantly shorter than that of other sites. Among 2119 case-patients, the non-multidrug-resistant pathogens were detected in 365 cases. The average increased LOS due to these bacterial infections was 12.2 days. infection caused significantly shorter LOS. The studied MDROs, namely, MRSA, VRE, ESBLs-, ESBLs-KP, CR-, CR-KP, CR-AB, and CR-PA were detected in 381 cases (18.0%). The average increased LOS due to these MDRO infections was 14 days. Comparing between different MDRO infections, we found that the increased LOS due to HAI caused by CR-PA (26.5 days) is longer than other MDRO infections (shorter than 19.8 days).
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http://dx.doi.org/10.1155/2019/2590563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500696PMC
November 2019

The Attributable Direct Medical Cost of Healthcare Associated Infection Caused by Multidrug Resistance Organisms in 68 Hospitals of China.

Biomed Res Int 2019 5;2019:7634528. Epub 2019 Mar 5.

Peking University First Hospital, Beijing 100034, China.

Healthcare associated infection (HAI) is known to increase the economic burden of patients while the medical cost due to MDRO HAI is even higher. Three hundred eighty-one multidrug resistance organisms (MDROs) healthcare associated infection (HAI) case-patients and three hundred eighty-one matched control-patients were identified between January and December in 2015. The average total hospitalization medical cost of the case group was $6127.65 and that of the control group was $2274.02. The difference between the case group and the control group was statistically significant (t = 21.07; P < 0.01). The attributable cost of MDRO HAI was $3853.63. The direct medical costs due to different MDRO infections were different. The increased medical costs of CR-AB, CR-KP, and CR-PA were significantly higher than that of MRSA, MRSE, ESBL E. coli, and ESBL Kp (P < 0. 05). Among the subitem expenses, the drug cost increased the most (the average cost was $1457.72), followed by the treatment fee and test fee; the differences were statistically significant (P < 0.01).
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http://dx.doi.org/10.1155/2019/7634528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425381PMC
July 2019

Diagnostic Potential for Circular RNAs in Gastric Carcinoma: a Meta-Analysis.

Clin Lab 2019 Mar;65(3)

Background: Circular RNAs (circRNAs) are potential, novel biomarkers for the early diagnosis of gastric carcinoma. Herein, a meta-analysis was conducted to assess the diagnostic potential for circRNAs in gastric carcinoma.

Methods: Online databases were searched for eligible studies. Study quality was judged using the Quality Assessment for Studies of Diagnostic Accuracy (QUADAS) checklist-II tool. STATA 12.0 and Meta-Disc 1.4 software were used for statistical analysis.

Results: Twelve studies consisting of 1,278 patients and 1,250 paired controls were considered for meta-analysis. The pooled sensitivity and specificity of circRNAs for gastric carcinoma were compared to normal controls and found to be 0.68 (95% CI: 0.66 - 0.71) and 0.70 (95% CI: 0.68 - 0.73), respectively. A corresponding area under the receiver operating characteristic curve of 0.78 was identified. Moreover, stratified analysis demonstrated an improved diagnostic value for circRNAs when tissue and plasma specimens were combined.

Conclusions: This meta-analysis demonstrates that circRNAs are promising biomarkers for gastric carcinoma.
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http://dx.doi.org/10.7754/Clin.Lab.2018.180810DOI Listing
March 2019

Double-Stranded RNAs High-Efficiently Protect Transgenic Potato from Leptinotarsa decemlineata by Disrupting Juvenile Hormone Biosynthesis.

J Agric Food Chem 2018 Nov 6;66(45):11990-11999. Epub 2018 Nov 6.

State Key Laboratory of Plant Genomics, Institute of Microbiology , Chinese Academy of Sciences , Beijing , China.

RNA interference (RNAi) has been developed for plant pest control. In this study, hairpin-type double-stranded RNA (dsRNA) targeting the juvenile hormone (JH) acid methyltransferase ( JHAMT) gene ( dsJHAMT) was introduced in potato plants via Agrobacterium-mediated transformation. The results indicated that the transcriptional RNA of dsJHAMT accumulated in the transgenic plants. The transcripts and proteins of the L. decemlineata JHAMT gene were significantly reduced in larvae feeding on dsJHAMT transgenic foliage. The dsJHAMT had a significant negative effect on the growth and development of L. decemlineata, especially resulting in less oviposition. Importantly, in the field trials, transgenic plants are high-efficiently protected from insect damage mainly because surviving insects laid fewer or no eggs. Even full protection from beetle damage can be acquired by continuously lowering insect population size at large scale in the field over the years. Therefore, the transgenic plants expressing dsJHAMT successfully provided an additional option for plant pest control.
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http://dx.doi.org/10.1021/acs.jafc.8b03914DOI Listing
November 2018

Exposure to Maternal Diabetes Mellitus Causes Renal Dopamine D Receptor Dysfunction and Hypertension in Adult Rat Offspring.

Hypertension 2018 10;72(4):962-970

From the Department of Cardiology, Chongqing Key Laboratory for Hypertension, Chongqing Institute of Cardiology, Chongqing Cardiovascular Clinical Research Center, Daping Hospital, The Third Military Medical University, P.R. China (H.L., C.C., L.G., Z.X., X.P., X.W., J.W., N.W., C.L., X.L., H.W., C.F., W.S., C.Z.).

Epidemiological and experimental studies suggest that maternal diabetes mellitus programs hypertension that is associated with impaired sodium excretion in the adult offspring. However, the underlying mechanisms are not clear. Because dopamine receptor function is involved in the pathogenesis of hypertension, we hypothesized that impaired renal dopamine D receptor function is also involved in the hypertension in offspring of maternal diabetes mellitus. Maternal diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (35 mg/kg) to pregnant Sprague-Dawley rats at day 0 of gestation. Compared with the offspring of mothers injected with citrate buffer (control mother offspring), the diabetic mother offspring (DMO) had increased systolic blood pressure and impaired D receptor-mediated diuresis and natriuresis, accompanied by increased renal PKC (protein kinase C) expression and activity, GRK-2 (G protein-coupled receptor kinase-2) expression, D receptor phosphorylation, D receptor/Gαs uncoupling, and loss of D receptor-mediated inhibition of Na-K-ATPase activity in renal proximal tubule cells from DMO. Inhibition of PKC reduced the increased GRK-2 expression and normalized D receptor function in primary cultures of renal proximal tubule cells from DMO. In addition, DMO, relative to control mother offspring, in vivo, had increased oxidative stress, indicated by decreased renal glutathione and increased renal malondialdehyde and urine 8-isoprostane. Normalization of oxidative stress with tempol also normalized the renal D receptor phosphorylation, D receptor-mediated diuresis and natriuresis, and blood pressure in DMO. Our present study indicates that maternal diabetes mellitus-programed hypertension in the offspring is caused by impaired renal D receptor function because of oxidative stress that is mediated by increased PKC-GRK-2 activity.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.118.10908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207228PMC
October 2018

Four-and-a-half LIM protein 1 promotes paclitaxel resistance in hepatic carcinoma cells through the regulation of caspase-3 activation.

J Cancer Res Ther 2018 Sep;14(Supplement):S767-S773

Department of Medical Oncology, Capital Medical University Cancer Center, Beijing Shijitan Hospital, Beijing 100038, P.R. China.

Background: Hepatocellular carcinoma (HCC) is the third leading cause of cancer deaths worldwide.

Aim: To investigate the mechanisms of paclitaxel resistance in hepatocellular carcinoma cells and find promising molecular target for HCC therapy.

Materials And Methods: To investigate the effects of FHL1 on chemo resistance in HCC cells, we generated FHL1 knock-down stable cell lines with HepG2 and SMMC7721 cells. Cell viability assay, colony formation and xenograft experiments assay were performed to detect effect of FHL1 on Paclitaxel or Oxaliplatin resistance in vitro and in vivo. Caspase activity assay was performed to explore the activation of caspase-3 and caspase-9 in paclitaxel treated FHL1-knockdown HepG2 cells.

Result: In the present study we have investigated that four-and-a-half LIM protein 1 (FHL1), which plays an important role in the development of cancer, is associated with both the chemo resistance of hepatocellular carcinomas cells in vitro and in vivo. Knockdown of FHL1 significantly enhanced the sensitivity of paclitaxel, but had no effects on sensitivity of oxaliplatin. Moreover, knockdown of FHL1 promoted the activation of caspase-3 and caspase-9, which were induced by paclitaxel. Interestingly, FHL1 negatively regulates the chemo resistance of HCC in xenografted nude mice.

Conclusion: FHL1 promote paclitaxel resistance in hepatocellular carcinomas cells through regulating apoptosis induced by paclitaxel, suggesting that FHL1 may be a promising molecular target for HCC therapy.
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http://dx.doi.org/10.4103/0973-1482.187304DOI Listing
September 2018

Simultaneous Editing of Two Copies of Confers Enhanced Transgene-Clean Plant Defense Against in Allotetraploid Upland Cotton.

Front Plant Sci 2018 28;9:842. Epub 2018 Jun 28.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

is an allotetraploid species, meaning that mutants that are difficult to be generated by classical approaches due to gene redundancy. The CRISPR/Cas9 genome editing system is a robust and highly efficient tool for generating target gene mutants, by which the genes of interest may be functionally dissected and applied through genotype-to-phenotype approaches. In this study, the CRISPR/Cas9 genome editing system was developed in through editing the gene. In T0 transgenic plants, lots of insertions and deletions (indels) in at the expected target site were detected in the allotetraploid cotton At or Dt subgenomes. The results of the PCR, T7EI digestion and sequencing analyses showed that the indels in gene can be stably transmitted to the next generation. Additionally, the indels in the At and Dt subgenomes were segregated in the T1 transgenic plants following Mendelian law, independing on the T-DNA segregation. Two homozygous -edited plants free of T-DNA were chosen by PCR and sequencing assays in the T1 plants, which were called transgene-clean editing plants and were designated and in the T2 lines showed higher resistance to infestation compared to the wild-type plants. Thus, the two transgene-clean edited lines can be used as a germplasm to breed disease-resistant cotton cultivars, possibly avoiding complex and expensive safety assessments of the transgenic plants.
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http://dx.doi.org/10.3389/fpls.2018.00842DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036271PMC
June 2018

The Genome Sequences of 90 Mushrooms.

Sci Rep 2018 07 2;8(1):9982. Epub 2018 Jul 2.

College of Biological Big Data, Yunnan Agriculture University, Kunming, 650201, Yunnan, China.

Macrofungus is defined as the fungus that grows an observable sporocarp. The sporocarps of many species are commonly called mushrooms and consumed by people all around the world as food and/or medicine. Most macrofungi belong to the divisions Basidiomycetes and Ascomycetes, which are estimated to contain more than 80,000 species in total. We report the draft genome assemblies of macrofungi (83 Basidiomycetes species and 7 Ascomycetes species) based on Illumina sequencing. The genome sizes of these species ranged from 27.4 Mb (Hygrophorus russula) to 202.2 MB (Chroogomphus rutilus). The numbers of protein-coding genes were predicted in the range of 9,511 (Hygrophorus russula) to 52,289 (Craterellus lutescens). This study provides the largest genomic dataset for macrofungi species. This resource will facilitate the artificial cultivation of edible mushrooms and the discovery of novel drug candidates.
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http://dx.doi.org/10.1038/s41598-018-28303-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028375PMC
July 2018

Seroprevalence of Neutralizing Antibodies against Human Adenovirus Type-5 and Chimpanzee Adenovirus Type-68 in Cancer Patients.

Front Immunol 2018 7;9:335. Epub 2018 Mar 7.

Department of Immunology, Tianjin Medical University Cancer Institute & Hospital, Tianjin, China.

Since the preclinical results about chimpanzee adenovirus serotype-68 (AdC68)-based vaccine showed an encouraging results, it reminded us that AdC68 may be a suitable cancer vaccine vector. Previous study indicated that the seroprevalence of neutralizing antibodies (NAbs) against adenovirus was different between cancer patients and healthy volunteers. Knowledge regarding the prevalence rates of AdC68 NAbs for cancer patients is lacking. Therefore, assessing the preexistence of NAbs against AdC68 in cancer patients could provide useful insights for developing future AdC68-based cancer vaccines. In this study, 440 patients with different pathological types of tumors and 204 healthy adult volunteers were enrolled to evaluate the NAbs against AdC68 and human adenovirus serotype-5 (AdHu5). The seroprevalence of NAbs against AdC68 was much lower than that against AdHu5 in cancer subjects (43.64 vs. 67.05%,  < 0.01). The seroprevalence rates of NAbs to AdC68 in the cancer subjects were statistically higher than those detected in the healthy adult volunteers (43.64 vs. 23.53%,  = 0.000). The seroprevalence rates of AdC68 NAbs were much lower in lung, laryngeal, esophageal, and cervical cancer patients compared with oropharyngeal, colon, and rectal cancer patients. Furthermore, the seroprevalence rates of AdC68 NAbs were much lower in lung adenocarcinoma patients than in lung squamous cell carcinoma patients (35.00 vs. 70.00%,  < 0.05). No significant difference in the AdC68 NAbs among patients with different clinical stages of cancer was detected. The percentage of NAbs against AdC68 was significantly lower than that against AdHu5 ( < 0.05) in stage-I, -II, and -III cancer patients. No significant difference between the percentage of NAbs against AdC68 and AdHu5 in the subjects with stage-IV cancer was detected. The study also demonstrated the distribution of AdHu5 and AdC68 NAb titers for the positive samples. It showed that very low NAb titers against AdC68 with respect to AdHu5 in both healthy subjects and cancer subjects, especially in lung, laryngeal, esophageal, gastric, and cervical carcinomas. Also, the titer of NAbs against AdC68 was significantly lower than that against AdHu5 in the same clinical stage and age group ( < 0.05). Taken together, the present study showed that NAbs against AdC68 is much lower than AdHu5, especially in lung adenocarcinoma, laryngeal cancer, esophageal cancer, and cervical cancer patients. These results provided strong support for candidating AdC68 as a suitable vector of cancer vaccines.
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http://dx.doi.org/10.3389/fimmu.2018.00335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845880PMC
March 2019

Multiple-site bleeding at pleural adhesions and massive hemothorax following percutaneous coronary intervention with stent implantation: A case report.

Exp Ther Med 2018 Mar 2;15(3):2351-2355. Epub 2018 Jan 2.

Department of Cardiovascular Medicine, Institute of Field Surgery, Daping Hospital of The Third Military Medical University, Chongqing 400042, P.R. China.

An elderly male patient with coronary heart disease underwent coronary angiography, which revealed bilateral severe coronary artery stenosis. Four stents were implanted, and at 5 h post-surgery, typical hemorrhagic shock appeared. Echocardiography verified heavy bleeding in the right thoracic cavity. Emergency angiography excluded injury or perforation of the coronary artery, aorta, right subclavian artery and brachiocephalic artery. Considering the patient's history of chronic obstructive pulmonary disease and severe cough during the operation, it was suspected that hemothorax was induced by a blood vessel rupture in the pleura. Video-assisted thoracic examination confirmed the tearing of pleural adhesion bands and bleeding at three sites in the pulmonary pleura and parietal pleura. According to the reported case, bleeding as a result of the laceration of pleural adhesions is an important cause of hemothorax that should be considered after exclusion of other common causes of pleural hemorrhage.
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http://dx.doi.org/10.3892/etm.2018.5685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795489PMC
March 2018

Abnormally expressed microRNA as auxiliary biomarkers for nasopharyngeal carcinoma: A meta-analysis.

Biomed Rep 2018 Jan 29;8(1):99-105. Epub 2017 Nov 29.

Laboratory of Biochemistry and Molecular Biology Research, Department of Clinical Laboratory, Fujian Provincial Key Laboratory of Tumor Biotherapy, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.

Aberrant expression of microRNA (miRNA) has been highlighted as a helpful indicator to aid in nasopharyngeal carcinoma (NPC) diagnosis. The present meta-analysis aimed to validate the efficacy of miRNA as potential biomarkers for NPC detection. Publication searches were conducted on the online PubMed and EMBASE databases from inception to June 2016. A bivariate meta-analysis was performed to generate the diagnostic parameters based on Meta-Disc 1.4 and Stata 12.0 programs. Sensitivity analysis and meta-regression tests were applied to trace heterogeneity sources among eligible studies. A total of six studies comprising 528 patients with NPC and 252 matched controls were enrolled. Results from the present meta-analysis demonstrated that miRNA testing achieved a pooled sensitivity of 0.78 [95% confidence interval (CI), 0.70-0.84] and specificity of 0.79 (95% CI, 0.73-0.84) in confirming NPC, corresponding to an area under the curve (AUC) value of 0.85. Additionally, the pooled diagnostic odds ratio was estimated to be 9.01 (95% CI, 5.62-14.44), along with a positive likelihood ratio of 2.81 (95% CI, 2.19-3.61) and negative likelihood ratio of 0.35 (95% CI, 0.28-0.44). Additionally, the stratified analyses revealed that paralleled testing of miRNA sustained a pooled accuracy superior compared with that of single miRNA testing (sensitivity, 0.88 vs. 0.70; specificity, 0.85 vs. 0.69; AUC, 0.95 vs. 0.75). Testing of miRNA harbors a moderate diagnostic efficacy and is acceptable as an auxiliary biomarker for NPC diagnosis.
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http://dx.doi.org/10.3892/br.2017.1026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5772640PMC
January 2018

Diagnostic efficacy of long non-coding RNA in human cancers: a meta-analysis study.

Oncotarget 2017 Nov 18;8(60):102291-102300. Epub 2017 Sep 18.

Laboratory of Biochemistry and Molecular Biology Research, Fujian Provincial Key Laboratory of Tumor Biotherapy, Department of Clinical Laboratory, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian, China.

Metastasis-associated lung adenocarcinoma transcript 1 () is one kind of long non-coding RNAs (lncRNAs) that has been recognized as a hallmark of the onset and development of several carcinomas. This study seek to meta-analyze the overall diagnostic efficacy of elevated expression profile for human cancers. Studies on the diagnostic performance of in cancers were retrieved by searching the online databases. The combined effect sizes were summarized using a bivariate meta-analysis model. Impacts of publication bias on the pooled effect sizes were assessed using "Duval and Tweedie nonparametric trim and fill method". Sensitivity analysis and meta-regression test were applied to deeply trace the heterogeneity sources among eligible studies. A total of 14 studies with 1342 cancer cases were included. The combined effect sizes showed that expression profiling conferred an estimated sensitivity of 0.69 (95% CI: 0.62-0.75) ( = 84.01%, < 0.001), specificity of 0.85 (95% CI: 0.79-0.90) ( = 87.95%, < 0.001) and AUC (area under curve) of 0.83 in distinguishing cancer patients from noncancerous contrasts. Moreover, stratified analysis depending on cancer type manifested that elevated harbored a promising efficacy in the diagnosis of pulmonary tumors (AUC = 0.90), digestive system tumors (AUC = 0.84), gynecologic cancers (AUC = 0.84) and nasopharyngeal carcinoma (AUC = 0.84), particularly in confirming the subtype of squamous carcinoma (AUC = 0.91) and non-small cell lung carcinoma (AUC = 0.88) in lung cancer. Other analyses based on test matrix and ethnicity also presented robust results. Collectively, elevated could be developed as an auxiliary molecular marker to aid in cancer diagnosis.
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http://dx.doi.org/10.18632/oncotarget.21013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5731954PMC
November 2017

Exogenous WWOX enhances apoptosis and weakens metastasis in CNE2 nasopharyngeal carcinoma cells through the intrinsic apoptotic pathway.

Int J Clin Exp Pathol 2017 1;10(10):10343-10351. Epub 2017 Oct 1.

Laboratory of Biochemistry and Molecular Biology Research, Fujian Provincial Key Laboratory of Tumor Biotherapy, Department of Clinical Laboratory, Fujian Cancer Hospital, Fujian Medical University Cancer Hospital Fuzhou, P. R. China.

The WW domain containing oxidoreductase (WWOX) has been postulated to behave as a putative tumor suppressor and that silencing of WWOX expression is linked to the carcinogenesis and progression of various carcinomas. The role of WWOX in nasopharyngeal carcinoma (NPC) remains unclear. Herein, we sought to evaluate the biological feature of WWOX restoration in human CNE2 NPC cells. In vitro experiments manifested that transiently overexpressed WWOX significantly suppressed proliferation as well as invasion and migration of the CNE2 cells. Of note, WWOX-induced apoptosis could be partly reversed by the selective caspase inhibitor, Z-VAD-FMK. Furthermore, immunoblotting analysis indicated that ectopic expression of WWOX could trigger the intrinsic apoptotic signaling pathway characterized by a down-regulation of Bcl-2 and Bcl-xL, and up-regulation of Bax and Cytochrome c along with a remarkable activation of the caspase cascades. Taken together, our data reveal that WWOX behaves as a potent tumor suppressor in CNE2 cells, possibly by enhancing apoptosis and weakening metastasis via the intrinsic apoptotic pathway.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965803PMC
October 2017

Effect of aldosterone on cochlear Af9 expression and hearing in guinea pig.

Acta Otolaryngol 2017 Sep 11;137(9):903-909. Epub 2017 Apr 11.

a Department of Otolaryngology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , China.

Conclusions: Af9 protein in cochlea may be closely related to endolymph regulation by aldosterone and thus may be involved in pathogenesis of endolymphatic hydrops (EH).

Objectives: EH is the pathological characteristic of Ménière's disease (MD). Aldosterone could induce EH, but its relationship with MD is still controversial. The aim of the present study is to investigate the Af9 protein expression in guinea pig cochlea and regulation of Af9 expression and cochlear function by aldosterone. The role of Af9 in pathogenesis of EH is discussed.

Methods: Thirty guinea pigs were randomly divided into two groups. The treatment group was intraperitoneally injected with aldosterone 0.1 mg/kg/d for 5 days, while the control group was done with saline. Hearing and histomorphology of cochlea were examined. In addition, expression of Af9 protein was studied.

Results: The hearing threshold of the treatment group was increased. EH was induced in 73% of guinea pigs in the treatment group, and no EH was found in the control group. Af9 protein was found in spiral limbus, stria vascularis, Reissner's membrane, organ of Corti and spiral ganglion in both groups. Af9 expression in cochlea decreased significantly at protein level after treatment by aldosterone.
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http://dx.doi.org/10.1080/00016489.2017.1309681DOI Listing
September 2017