Publications by authors named "Xiaoli Fu"

54 Publications

Validating machine learning models for the prediction of labour induction intervention using routine data: a registry-based retrospective cohort study at a tertiary hospital in northern Tanzania.

BMJ Open 2021 Dec 2;11(12):e051925. Epub 2021 Dec 2.

College of Public Health, Zhengzhou University, Zhengzhou, China

Objectives: We aimed at identifying the important variables for labour induction intervention and assessing the predictive performance of machine learning algorithms.

Setting: We analysed the birth registry data from a referral hospital in northern Tanzania. Since July 2000, every birth at this facility has been recorded in a specific database.

Participants: 21 578 deliveries between 2000 and 2015 were included. Deliveries that lacked information regarding the labour induction status were excluded.

Primary Outcome: Deliveries involving labour induction intervention.

Results: Parity, maternal age, body mass index, gestational age and birth weight were all found to be important predictors of labour induction. Boosting method demonstrated the best discriminative performance (area under curve, AUC=0.75: 95% CI (0.73 to 0.76)) while logistic regression presented the least (AUC=0.71: 95% CI (0.70 to 0.73)). Random forest and boosting algorithms showed the highest net-benefits as per the decision curve analysis.

Conclusion: All of the machine learning algorithms performed well in predicting the likelihood of labour induction intervention. Further optimisation of these classifiers through hyperparameter tuning may result in an improved performance. Extensive research into the performance of other classifier algorithms is warranted.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjopen-2021-051925DOI Listing
December 2021

Gene mining, codon optimization and analysis of binding mechanism of an aldo-keto reductase with high activity, better substrate specificity and excellent solvent tolerance.

PLoS One 2021 2;16(12):e0260787. Epub 2021 Dec 2.

College of Chemical Engineering, Huaqiao University, Xiamen, China.

The biosynthesis of chiral alcohols has important value and high attention. Aldo-keto reductases (AKRs) mediated reduction of prochiral carbonyl compounds is an interesting way of synthesizing single enantiomers of chiral alcohols due to the high enantio-, chemo- and regioselectivity of the enzymes. However, relatively little research has been done on characterization and apply of AKRs to asymmetric synthesis of chiral alcohols. In this study, the AKR from Candida tropicalis MYA-3404 (C. tropicalis MYA-3404), was mined and characterized. The AKR shown wider optimum temperature and pH. The AKR exhibited varying degrees of catalytic activity for different substrates, suggesting that the AKR can catalyze a variety of substrates. It is worth mentioning that the AKR could catalytic reduction of keto compounds with benzene rings, such as cetophenone and phenoxyacetone. The AKR exhibited activity on N,N-dimethyl-3-keto-3-(2-thienyl)-1-propanamine (DKTP), a key intermediate for biosynthesis of the antidepressant drug duloxetine. Besides, the AKR still has high activity whether in a reaction system containing 10%-30% V/V organic solvent. What's more, the AKR showed the strongest stability in six common organic solvents, DMSO, acetonitrile, ethyl acetate, isopropanol, ethanol, and methanol. And, it retains more that 70% enzyme activity after 6 hours, suggesting that the AKR has strong solvent tolerance. Furthermore, the protein sequences of the AKR and its homology were compared, and a 3D model of the AKR docking with coenzyme NADPH were constructed. And the important catalytic and binding sites were identified to explore the binding mechanism of the enzyme and its coenzyme. These properties, predominant organic solvents resistance and extensive substrate spectrum, of the AKR making it has potential applications in the pharmaceutical field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0260787PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638942PMC
December 2021

Effects of SNPs in SOD2 and SOD3 interacted with fluoride exposure on the susceptibility of dental fluorosis.

Int J Hyg Environ Health 2022 Jan 2;239:113879. Epub 2021 Nov 2.

School of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China. Electronic address:

A total of 649 children aged 7-13 years of age were recruited in a cross-sectional study in Tongxu County, China (2017) to assess the effects of interaction between single nucleotide polymorphisms (SNPs) in SOD2 and SOD3 gene and fluoride exposure on dental fluorosis (DF) status. Associations between biomarkers and DF status were evaluated. Logistic regression suggested that the risk of DF in children with rs10370 GG genotype and rs5746136 TT genotype was 1.89-fold and 1.72-fold than that in children with TT/CC genotype, respectively. Increased T-SOD activity was associated with a lower risk of DF (OR = 0.99). The rs2855262*rs10370*UF model was regarded as the optimal interaction model in generalized multifactor dimensionality reduction analyses. Our findings suggested that rs4880 and rs10370 might be useful genetic markers for DF, and there might be interactions among rs10370 in SOD2, rs2855262 in SOD3, and fluoride exposure on DF status.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijheh.2021.113879DOI Listing
January 2022

Effects of continuing nursing care under cognitive behavioral intervention on psychological state and living quality in patients with double J catheter after ureterolithiasis surgery.

Am J Transl Res 2021 15;13(9):10721-10728. Epub 2021 Sep 15.

Department of Urology, The Second Affiliated Hospital of Hainan Medical University Haikou 570311, Hainan, China.

Objective: The effects of continuing nursing care under cognitive behavioral intervention on the psychological state and living quality of patients with double J catheter after ureterolithiasis surgery were analyzed in this research.

Methods: The research subjects in this study were 142 patients who were discharged from our hospital with a double J tube after ureterolithiasis surgeries from June 2019 to June 2020. According to the patient care plan, the subjects were divided into the control group (n=71) and the observation group (n=71) and respectively treated with routine basic nursing measures and continuing nursing care under cognitive-behavior intervention. Subsequently, the psychological state, living quality and the complications that occurred were compared between the two groups.

Results: The depression and anxiety scores of the two groups after 4 weeks of intervention, comparing to thoes before intervention, were seen with remarkably decreased significance (). The scores of living quality and self-care quality of patients in the observation group were obviously higher than those in the control group (). In addition, the incidence of complications in the observation group was critically lower than that in control group ().

Conclusion: Continuous nursing care under cognitive behavioral intervention can substantially improve the psychological state, living quality and self-care ability of patients with double J tube after ureterolithiasis surgery, and reduce the incidence of complications, which is worthy of clinic promotion.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507067PMC
September 2021

Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure.

Ecotoxicol Environ Saf 2021 Dec 21;225:112796. Epub 2021 Sep 21.

Department of Environmental Health, School of Public Health, Zhengzhou University, Environment and Health Innovation Team, Zhengzhou, Henan 450001, PR China. Electronic address:

To identify the role of the Hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure. Environmental response genes (ERGs) of bone injury induced by fluoride exposure were obtained from the Comparative Toxicogenomics Database, and annotated by STRING for KEGG pathway enrichment analysis. The CCK-8 kit was used to measure the proliferation of ATDC5 cells. The malondialdehyde (MDA), total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-PX) levels in ATDC5 cells were measured using oxidative stress detection kit. Western blot analysis was used to measure the p-MST1/2, p-LATS1/2, and p-YAP/YAP1 expression levels in the Hippo pathway and the COL2A1, ACAN and MMP13 expression levels in the cartilage matrix. Localizations of YAP1 and COL2A1 proteins in chondrocytes were performed using cell immunofluorescence. Continuous data from the multiple groups were compared using one-way analysis of variance, and then the differences between groups were tested with Dunnett's t-test, with the test level α = 0.05. The 145 ERGs of bone injury induced by fluoride exposure were identified, and KEGG enrichment analysis revealed Hippo signaling pathways significantly related to bone injury. A CCK-8 assay revealed that the viability of the ATDC5 cells was significantly decreased with increased fluorine concentration. The MDA content in 20 mg/L sodium fluoride (NaF) exposure group was significantly higher than that in the control group, the T-SOD, T-AOC and GSH-PX activities in 15 and 20 mg/L NaF exposure groups were significantly lower than those in the control group (P < 0.05). Western blot results showed the protein levels of p-MST1/2, p-LATS1/2 and p-YAP1 in 15 and 20 mg/L NaF exposure groups were significantly lower than those in the control group, while the YAP1 protein level in 20 mg/L NaF group was significantly higher than that in the control group. The COL2A1 and ACAN proteins in 20 mg/L NaF group were significantly decreased, while the MMP13 protein level in 15 and 20 mg/L NaF groups were significantly increased (P < 0.05). It was observed that the expression of YAP1 protein expression level in the cytoplasm decreased with the increased fluoride exposure, whereas that the expression level of YAP1 protein in the nucleus increased. Fluoride inhibited the proliferation of ATDC5 cells, induced oxidative stress, inhibited the activity of the Hippo pathway, and eventually led to cartilage matrix degradation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2021.112796DOI Listing
December 2021

Eight-year follow-up outcome of subthalamic deep brain stimulation for Parkinson's disease: Maintenance of therapeutic efficacy with a relatively low levodopa dosage and stimulation intensity.

CNS Neurosci Ther 2021 11 5;27(11):1366-1373. Epub 2021 Aug 5.

Department of Neurology, Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Aims: This follow-up study aimed to examine the 8-year efficacy and safety of subthalamic nucleus (STN) deep brain stimulation (DBS) for patients with Parkinson's disease (PD) in southern China.

Methods: The follow-up data of 10 patients with PD undergoing STN-DBS were analyzed. Motor symptoms were assessed before and 1, 3, 5, and 8 years after the surgery with stimulation-on in both off-medication (off-med) and on-medication (on-med) status using the Unified Parkinson's disease Rating Scale Part III. The quality of life was assessed using the 39-item Parkinson's Disease Questionnaire. The sleep, cognition, and emotion were evaluated using a series of nonmotor scales. Levodopa equivalent daily dose (LEDD) and stimulation parameters were recorded at each follow-up.

Results: The motor symptoms were improved by 50.9%, 37.7%, 36.7%, and 37.3% in 1, 3, 5, and 8 years, respectively, in the off-med / stimulation-on status compared with the baseline. The quality of life improved by 39.7% and 56.1% in 1 and 3 years, respectively, but declined to the preoperative level thereafter. The sleep, cognition, and emotion were mostly unchanged. LEDD reduced from 708.1 ± 172.5 mg to 330 ± 207.8 mg in 8 years. The stimulation parameters, including amplitude, pulse width, and frequency, were 2.77 ± 0.49 V, 71.3 ± 12.8 μs, and 121.5 ± 21 Hz, respectively, in 8 years.

Conclusion: Long-term therapeutic efficacy of STN-DBS could be achieved even with relatively low stimulation intensity and medication dosage for PD patients in southern China. Motor improvement and medication reduction were maintained through the 8-year follow-up, but improvement in quality of life lasted for only 3 years. No definite changes was found in nonmotor symptoms after STN-DBS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/cns.13713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504521PMC
November 2021

Spatial variation, water quality, and health risk assessment of trace elements in groundwater in Beijing and Shijiazhuang, North China Plain.

Environ Sci Pollut Res Int 2021 Oct 3;28(40):57046-57059. Epub 2021 Jun 3.

Department of Hydraulic Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, China.

Metal(loid)s pollution of groundwater in northern China is of great concern due to the increasing shortage of fresh water resources. In the present study, total 159 of groundwater samples were collected from the Miyun-Huairou-Shunyi (MHS) districts in Beijing city and the Hutuo River Plain (HRP) in Shijiazhuang city. Nineteen trace elements dissolved in groundwater were measured. Results showed that Al (12.3 %), Mn (5.3%), Zn (1.8%), As (1.8%), and Pb (1.8%) in the MHS samples, and Mn (2.2%) in the HRP samples exceeded their standard threshold of WHO and China. Exceedance of trace elements was attributed to both geochemical background and local human activities. Human health risk assessment showed that local consumers were exposed at a low level of health risk, except in specific area with a high level of arsenic. Elements of arsenic and chromium were important risk contributors in the two regions. The risk of oral exposure was greater than that of skin uptake. Children were more susceptible to non-carcinogenic risk and less to carcinogenic risk than adults. A Nemerow index and CRITIC-weighted WQI were applied to classify groundwater quality. The results from the two methods were comparable to a large extend. More population living in plain rather than mountain resulted in a gradual deterioration trend of groundwater quality from mountain to plain. The samples with poor water quality were almost collected in the area with heavy industrial and agricultural activities. The CRITIC-weighted WQI was recommended for groundwater quality assessment. A simple classification criterion was reformulated based on the MHS hazard index analysis. The groundwaters in the two research fields were not seriously polluted, but potential risks should not be ignored.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14557-3DOI Listing
October 2021

Ligand based 3D-QSAR model, pharmacophore, molecular docking and ADME to identify potential fibroblast growth factor receptor 1 inhibitors.

J Biomol Struct Dyn 2021 Mar 18:1-14. Epub 2021 Mar 18.

College of Life Sciences, Sichuan Agricultural University, Ya'an, China.

The FGF/FGFR system may affect tumor cells and stromal microenvironment through autocrine and paracrine stimulation, thereby significantly promoting oncogene transformation and tumor growth. Abnormal expression of FGFR1 in cells is considered to be the main cause of tumorigenesis and a potential target for the treatment of cancer. In this study, a combination of structure-based drug carriers and molecular docking-based virtual screening was used to screen new potential FGFR1 inhibitors. Forty eight known inhibitors were collected to establish 3 D-QSAR models and pharmacophore models, investigate the relationship between the activity and conformation of compounds, and verify the efficiency of pharmacophore. In Accelrys Discovery Studio 2016, the ZINC database was filtered by Lipinski's Rule of Five and SMART's filtration. Then, Hypo01 was used for virtual screening of ZINC database. Compounds with predicted activity values less than 1 μM were molecularly docked with FGFR1 protein crystals, the docking results were observed, and the interaction between compounds and targets was studied. The absorption, distribution, metabolism and excretion (ADME) and toxicity of potential inhibitors were studied, and a compound with new structural scaffolds were obtained. It could be further studied to explore their better therapeutic effects.Communicated by Ramaswamy H. Sarma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/07391102.2021.1899049DOI Listing
March 2021

The scale and structure of government financial investment in traditional medicine based on optimal efficiency: evidence from public traditional Chinese medicine hospitals (PTHs) of Henan province, China.

BMC Health Serv Res 2021 Feb 26;21(1):182. Epub 2021 Feb 26.

College of Public Health, Zhengzhou University, 100 Science Avenue Gaoxin District, Zhengzhou, People's Republic of China.

Background: Traditional medicine has been widely used to address relatively common illnesses. In this regard, Chinese government has been continually topping up its investments on public Traditional Chinese Medicine hospitals (PTHs) in recent years. This study aimed to assess the optimal scales and structure of the investments in Henan province by analyzing the contribution of Government Financial Investment (GFI) to the efficiency and revenue growth of PTHs as well as recommending proper investment strategies for implementation to policy-makers.

Methods: This study was a panel data study, conducted in Henan Province, China. By collecting 143 PTHs' operational data from the year 2005 to 2017, Barro Economic Growth (BEG) model, Stochastic Frontier Analysis (SFA) and Vector Autoregressive (VAR) model were used to assess the efficiency and PTHs revenue.

Results: The study observed the positive contribution of GFI to PTHs' revenue growth (average MPG = 2.84), indicating that the GFI had not reached the required optimal level of "Barro Law". In order to maximize the input-output efficiency, the scales of GFI on Grade III, Grade II A, Grade II B PTHs need to be increased by - 5.96, 4.88 and 11.51%, respectively. The third year following the first investment may be a more essential period for conducting an effective GFI evaluation in Henan Province.

Conclusions: GFI on PTHs usually has a long-term impact on PTHs. Governments can adjust its GFI policy so as to maximize the input-output efficiency.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12913-021-06185-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908777PMC
February 2021

TCGA dataset screening for genes implicated in endometrial cancer using RNA-seq profiling.

Cancer Genet 2021 06 4;254-255:40-47. Epub 2021 Feb 4.

College of Public Health, Zhengzhou University, Zhengzhou 450001, China; China Canada Medical and Health Science Association, Toronto L3R 1A3, Canada. Electronic address:

The molecular basis of the mechanism and the potential biomarkers of endometrial cancer (EC) remain to be studied. In the present study, we hypothesized that the comprehensive characterization of transcriptional changes in EC could help achieve this aim. By taking advantage of RNA-seq data from The Cancer Genome Atlas, we determined the profile of differently expressed genes (DEGs) between EC tumor tissues and normal samples. On this basis, we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways enrichment analyses. The interacting partners for each of the DEGs were explored and a protein-protein interaction network was constructed. Consequently, 10 hub genes were identified and their association with mortality in EC patients was investigated. The genes, AURKA, CENPA, and KIF2C, were found to be potential biomarkers for EC with a significant prognostic effect. Our work provided a basis for EC studies in both biological and clinical settings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cancergen.2021.01.011DOI Listing
June 2021

Prognostic role of SIRT6 in gastrointestinal cancers: a meta-analysis.

Open Med (Wars) 2020 21;15(1):358-365. Epub 2020 Apr 21.

Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.

Sirtuin 6 (SIRT6) plays a critical role in the progression and development of gastrointestinal cancers. However, the association between SIRT6 expression and clinicopathological parameters and prognosis in gastrointestinal cancer patients remains inconclusive. Consequently, we conducted this meta-analysis to evaluate the importance of SIRT6 expression in various types of gastrointestinal cancers. PubMed, EMBASE, and Web of Science databases were systematically searched to screen the relevant literature. The reported or estimated hazard ratio (HR) and odds ratio (OR) and their corresponding 95% confidence interval (CI) were pooled to assess the strength of the association. Nine studies involving 867 patients were included in the meta-analysis. Overall analysis showed that high SIRT6 expression was related to better overall survival in gastrointestinal cancers (HR = 0.62, 95% CI = 0.47-0.82). High SIRT6 expression was also related to a favorable tumor node metastasis (TNM) stage (OR = 0.44, 95% CI = 0.28-0.70) among gastrointestinal cancer patients. Our meta-analysis revealed that high SIRT6 expression might be a potential biomarker predicting better prognosis in gastrointestinal cancers, which may offer options for gastrointestinal cancer treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/med-2020-0403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712292PMC
April 2020

Photo-ammonification of low molecular weight dissolved organic nitrogen by direct and indirect photolysis.

Sci Total Environ 2021 Apr 19;764:142930. Epub 2020 Oct 19.

School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.

The photo-ammonification process plays a crucial role in the transformation of dissolved organic nitrogen (DON) to dissolved inorganic nitrogen (DIN). However, previous studies have primarily focused on DON biotransformation than on abiotic processes. This study investigated the photo-ammonification process of nine model low molecular weight (LMW) DON molecules (e.g., amino acids, nucleotides, and urea) under the influence of different light sources. The results showed that photo-ammonification of model DON was mainly induced by UV light, while negligible contribution by visible light was found. Depending on their molecular structures, amino acids yielded different ammonia amounts, whereas negligible photo-ammonification was observed for nucleotides and urea. As for the reactive species, OH promoted ammonia yields of all the model amino acids; CDOM contributed to the photo-ammonification of six amino acids; O only had a positive impact on ammonification of tryptophan, histidine, and tyrosine; and CO accelerated ammonia generation from histidine and methionine. In natural water samples, tryptophan, tyrosine, histidine, and methionine generated significant ammonia. OH and O were speculated as the contributing reactive species based on kinetic studies as well as significant fluorescent humic-like and tyrosine-like substances degradation in irradiated samples compared to the raw samples characterized by the EEM-PARAFAC analysis. The negative linear correlations between photo-ammonification rates and the E-E of the amino acids emphasized the importance of the role of the molecular structure. Overall, these results revealed the LMW DON photo-ammonification mechanism in sunlit surface waters and highlighted its significance in the nitrogen biogeochemical cycle as well as water quality management.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2020.142930DOI Listing
April 2021

Trade-offs between xylem water and carbohydrate storage among 24 coexisting subtropical understory shrub species spanning a spectrum of isohydry.

Tree Physiol 2021 03;41(3):403-415

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.

Hydraulic capacitance and carbohydrate storage are two drought adaptation strategies of woody angiosperms. However, we currently lack information on their associations and how they are associated with species' degree of isohydry. We measured total stem xylem nonstructural carbohydrate (NSC) concentration in the dry and wet seasons, xylem hydraulic capacitance, native leaf water potentials, pressure-volume curve parameters and photosynthetic performance in 24 woody understory species differing in their degree of isohydry. We found a trade-off between xylem water and carbohydrate storage both in storage capacitance and along a spectrum of isohydry. Species with higher hydraulic capacitance had lower native NSC storage. The less isohydric species tended to show greater NSC depletion in the dry season and have more drought-tolerant leaves. In contrast, the more isohydric species had higher hydraulic capacitance, which may enhance their drought avoidance capacity. In these species, leaf flushing in the wet season and higher photosynthetic rates in the dry season resulted in accumulation rather than depletion of NSC in the dry season. Our results provide new insights into the mechanisms through which xylem storage functions determine co-occurring species' drought adaptation strategies and improve our capacity to predict community assembly processes under drought.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/treephys/tpaa138DOI Listing
March 2021

Distribution, sources, and decomposition of soil organic matter along a salinity gradient in estuarine wetlands characterized by C:N ratio, δ C-δ N, and lignin biomarker.

Glob Chang Biol 2021 Jan 6;27(2):417-434. Epub 2020 Nov 6.

School of Environmental and Chemical Engineering, Foshan University, Foshan, Guangdong, China.

Despite increasing recognition of the critical role of coastal wetlands in mitigating climate change, sea-level rise, and salinity increase, soil organic carbon (SOC) sequestration mechanisms in estuarine wetlands remain poorly understood. Here, we present new results on the source, decomposition, and storage of SOC in estuarine wetlands with four vegetation types, including single Phragmites australis (P, habitat I), a mixture of P. australis and Suaeda salsa (P + S, habitat II), single S. salsa (S, habitat III), and tidal flat (TF, habitat IV) across a salinity gradient. Values of δ C increased with depth in aerobic soil layers (0-40 cm) but slightly decreased in anaerobic soil layers (40-100 cm). The δ N was significantly enriched in soil organic matter at all depths than in the living plant tissues, indicating a preferential decomposition of N-enriched organic components. Thus, the kinetic isotope fractionation during microbial degradation and the preferential substrate utilization are the dominant mechanisms in regulating isotopic compositions in aerobic and anaerobic conditions, respectively. Stable isotopic (δ C and δ N), elemental (C and N), and lignin composition (inherited (Ad/Al)s and C/V) were not completely consistent in reflecting the differences in SOC decomposition or accumulation among four vegetation types, possibly due to differences in litter inputs, root distributions, substrate quality, water-table level, salinity, and microbial community composition/activity. Organic C contents and storage decreased from upstream to downstream, likely due to primarily changes in autochthonous sources (e.g., decreased onsite plant biomass input) and allochthonous materials (e.g., decreased fluvially transported upland river inputs, and increased tidally induced marine algae and phytoplankton). Our results revealed that multiple indicators are essential to unravel the degree of SOC decomposition and accumulation, and a combination of C:N ratios, δ C, δ N, and lignin biomarker provides a robust approach to decipher the decomposition and source of sedimentary organic matter along the river-estuary-ocean continuum.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/gcb.15403DOI Listing
January 2021

The association between polymorphisms in miRNA and the cholinesterase activity of workers in an omethoate-exposed environment.

Int J Environ Health Res 2020 Sep 22:1-10. Epub 2020 Sep 22.

College of Public Health, Zhengzhou University, Zhengzhou, China.

To explore the association between polymorphisms in microRNAs (miRNAs) and the cholinesterase (ChE) activity in omethoate-exposed workers, we recruited 180 omethoate-exposed workers and 115 controls to measure their ChE activity using acetylcholine and dithio-bis-(nitrobenzoic acid) and genotype susceptible SNPs in their miRNA by time-of-flight mass spectrometry. ChE activity in the exposure group was lower than that in the control group ( < 0.001). The analysis of covariance result showed that ChE activity was lower in the (- -/- T) genotype in rs111456995 (1.97 ± 0.47) than in the TT genotype (2.23 ± 0.59) of the exposure group ( = 0.004). Multivariate linear regression was performed to find influencing factors on ChE activity, and variables kept in the model included omethoate exposure ( = -1.094, < 0.001), gender ( = -0.381, < 0.001), rs111456995 (- -/- T)( = -0.248, < 0.001), and drinking ( = 0.258, =0.019). The results suggest that individuals carrying a (- -/- T) genotype in rs111456995 were more susceptible to damage in their cholinesterase induced by omethoate exposure.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/09603123.2020.1820453DOI Listing
September 2020

Mycorrhizal and environmental controls over root trait-decomposition linkage of woody trees.

New Phytol 2021 01 1;229(1):284-295. Epub 2020 Sep 1.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Traits are critical in predicting decomposition that fuels carbon and nutrient cycling in ecosystems. However, our understanding of root trait-decomposition linkage, and especially its dependence on mycorrhizal type and environmental context, remains limited. We explored the control of morphological and chemical (carbon- and nutrient-related) traits over decomposition of absorptive roots in 30 tree species associated with either arbuscular mycorrhizal (AM) or ectomycorrhizal (ECM) fungi in temperate and subtropical forests in China. Carbon-related traits (acid-unhydrolysable residue (AUR) and cellulose concentrations) had predominant control of root decomposition in AM species while nutrient-related traits (magnesium concentration) predominately controlled that in ECM species. Thicker absorptive roots decomposed faster in AM species as a result of their lower AUR concentrations, but more slowly in ECM angiosperm species potentially as a result of their higher magnesium concentrations. Root decomposition was linked to root nutrient economy in both forests while root diameter-decomposition coordination emerged only in the subtropical forest where root diameter and decomposition presented similar cross-species variations. Our findings suggest that root trait-decomposition linkages differ strongly with mycorrhizal type and environment, and that root diameter can predict decomposition but in opposing directions and with contrasting mechanisms for AM and ECM species.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.16844DOI Listing
January 2021

Diversity-decomposition relationships in forests worldwide.

Elife 2020 06 26;9. Epub 2020 Jun 26.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.

Plant species diversity affects carbon and nutrient cycling during litter decomposition, yet the generality of the direction of this effect and its magnitude remains uncertain. With a meta-analysis including 65 field studies across the Earth's major forest ecosystems, we show here that decomposition was faster when litter was composed of more than one species. These positive biodiversity effects were mostly driven by temperate forests but were more variable in other forests. Litter mixture effects emerged most strongly in early decomposition stages and were related to divergence in litter quality. Litter diversity also accelerated nitrogen, but not phosphorus release, potentially indicating a decoupling of nitrogen and phosphorus cycling and perhaps a shift in ecosystem nutrient limitation with changing biodiversity. Our findings demonstrate the importance of litter diversity effects for carbon and nutrient dynamics during decomposition, and show how these effects vary with litter traits, decomposer complexity and forest characteristics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.55813DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402676PMC
June 2020

The p.R206C Mutation in MYO7A Leads to Autosomal Dominant Nonsyndromic Hearing Loss.

ORL J Otorhinolaryngol Relat Spec 2020 19;82(4):181-187. Epub 2020 May 19.

Department of Otorhinolaryngology-Head and Neck Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Background: Dominant mutations in MYO7A may lead to nonsyndromic deafness DFNA11. A p.R206C variant in MYO7A has previously been reported in a small deaf family from Taiwan but with ambiguous pathogenicity and inheritance pattern.

Aims/objectives: Our study aims to clarify the pathogenicity of this variant by clinical characterization and genetic analysis of a separate autosomal dominant deaf family harboring this variant in mainland China.

Materials And Methods: Auditory features of hearing loss were characterized in representative affected family members. Mutation screening was performed by targeted next-generation sequencing of 138 known deafness genes in the proband. Candidate pathogenic mutations were confirmed by Sanger sequencing in family members and ethnically matched controls.

Results: Consistent with typical DFNA11 phenotype, the affected family members in this study showed delayed-onset, progressive hearing loss affecting mostly high frequencies. Targeted next-generation sequencing identified a p.R206C mutation in MYO7A as the only candidate pathogenic mutation cosegregating with the hearing phenotype. This mutation is not seen in 200 Chinese Han normal-hearing controls.

Conclusions And Significance: The recurrent p.R206C variant in MYO7A is pathogenic and is likely in a mutation hot spot or due to a founder effect. Reports of such rare variants in multiple patients or families may facilitate exploitation of its pathogenicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000506208DOI Listing
March 2021

Enhancing global health engagement in 21st century China.

BMJ Glob Health 2020 19;5(3):e002194. Epub 2020 Mar 19.

College of Public Health, Zhengzhou University, Zhengzhou, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjgh-2019-002194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103855PMC
June 2021

Targeting KDM5 Demethylases: Inhibition and Degradation.

Curr Top Med Chem 2020;20(4):261-263

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2174/156802662004200304124340DOI Listing
December 2020

Below-ground determinants and ecological implications of shrub species' degree of isohydry in subtropical pine plantations.

New Phytol 2020 06 12;226(6):1656-1666. Epub 2020 May 12.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modelling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

The degree of plant iso/anisohydry is a popular framework for characterising species-specific drought responses. However, we know little about associations between below-ground and above-ground hydraulic traits as well as the broader ecological implications of this framework. For 24 understory shrub species in seasonally dry subtropical coniferous plantations, we investigated contributions of the degree of isohydry to species' resource economy strategies, abundance, and importance value, and quantified the hydraulic conductance (K ) of above-ground and below-ground organs, magnitude of deep water acquisition (WA ), shallow absorptive root traits (diameter, specific root length, tissue density), and resource-use efficiencies (A , maximum photosynthesis rate; PNUE, photosynthetic nitrogen-use efficiency). The extreme isohydric understory species had lower wood density (a proxy for higher growth rates) because their higher WA and whole-plant K allowed higher A and PNUE, and thus did not necessarily show lower abundance and importance values. Although species' K was coordinated with their water foraging capacity in shallow soil, the more acquisitive deep roots were more crucial than shallow roots in shaping species' extreme isohydric behaviour. Our results provide new insights into the mechanisms through which below-ground hydraulic traits, especially those of deep roots, determine species' degree of isohydry and economic strategies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.16502DOI Listing
June 2020

Endocytosis mechanism in physiologically-based pharmacokinetic modeling of nanoparticles.

Toxicol Appl Pharmacol 2019 12 31;384:114765. Epub 2019 Oct 31.

School of Energy Science and Engineering, Central South University, Changsha 410083, Hunan, China; XiangYa School of Public Health, Central South University, Changsha 410008, China; School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China; School of Architecture and Art, Central South University, Changsha 410083, Hunan, China. Electronic address:

Background: The physiologically based pharmacokinetic (PBPK) model is a useful tool to predict the pharmacokinetics of various types of nanoparticles (NPs). The endocytosis mechanism plays a key role in pharmacokinetics of NPs. However, the effect of endocytosis mechanism both in the blood and tissue are seldom considered in PBPK model.

Objectives: To investigate the biodistribution of intravenously injected pegylated AuNPs in mice and human using PBPK model considering the endocytosis mechanism both in the blood and tissue.

Methods: Taking polyethylene glycol-coated gold nanoparticles (AuNPs) as an example, we developed a PBPK model to explore biodistribution of different size AuNPs. In the model, we considered the role of endocytosis mechanism both in the blood and tissue. In addition, the size-dependent permeability coefficient, excretion rate constant, phagocytic capacity, uptake rate, and release rate were derived from literatures. The mice PBPK model was extrapolated to the human by changing physiology parameters and the number of phagocytic cell (PCs).

Results: AuNPs were primarily distributed in the blood, liver, and spleen regardless of particle size, and almost all captured by the PCs in the liver and spleen, while few was captured in the blood. There are more organ distribution and longer circulation for smaller NPs. The 24-h accumulation of AuNPs decreased with increasing size in the most organ, while the accumulation of AuNPs showed an inverted U-shaped curve in the liver and slight U-shaped curve in the blood. The human results of model-predicted displayed a similar tendency with those in mice. Size, partition coefficients, and body weight were the key factors influencing the organ distribution of AuNPs.

Conclusions: The size played an important role on the distribution and accumulation of AuNPs in various tissues. Our PBPK model was well predicted the NPs distribution in mice and human. A better understanding of these mechanisms could provide effective guides for nanomedine delivery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.taap.2019.114765DOI Listing
December 2019

Linking reliance on deep soil water to resource economy strategies and abundance among coexisting understorey shrub species in subtropical pine plantations.

New Phytol 2020 01 29;225(1):222-233. Epub 2019 Jul 29.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Strategies for deep soil water acquisition (WA ) are critical to a species' adaptation to drought. However, it is unknown how WA determines the abundance and resource economy strategies of understorey shrub species. With data from 13 understorey shrub species in subtropical coniferous plantations, we investigated associations between the magnitude of WA , the seasonal plasticity of WA , midday leaf water potential (Ψ ), species abundance and resource economic traits across organs. Higher capacity for WA was associated with higher intrinsic water use efficiency, but was not necessary for maintaining higher Ψ in the dry season nor was it an ubiquitous trait possessed by the most common shrub species. Species with higher seasonal plasticity of WA had lower wood density, indicating that fast species had higher plasticity in deep soil resource acquisition. However, the magnitude and plasticity of WA were not related to shallow fine root economy traits, suggesting independent dimensions of soil resource acquisition between deep and shallow soil. Our results provide new insights into the mechanisms through which the magnitude and plasticity of WA interact with shallow soil and aboveground resource acquisition traits to integrate the whole-plant economic spectrum and, thus, community assembly processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.16027DOI Listing
January 2020

Coordination and trade-offs between leaf and stem hydraulic traits and stomatal regulation along a spectrum of isohydry to anisohydry.

Plant Cell Environ 2019 07 22;42(7):2245-2258. Epub 2019 Mar 22.

Department of Biology, Western Oregon University, Monmouth, Oregon.

The degree of plant iso/anisohydry, a widely used framework for classifying species-specific hydraulic strategies, integrates multiple components of the whole-plant hydraulic pathway. However, little is known about how it associates with coordination of functional and structural traits within and across different organs. We examined stem and leaf hydraulic capacitance and conductivity/conductance, stem xylem anatomical features, stomatal regulation of daily minimum leaf and stem water potential (Ψ), and the kinetics of stomatal responses to vapour pressure deficit (VPD) in six diverse woody species differing markedly in their degree of iso/anisohydry. At the stem level, more anisohydric species had higher wood density and lower native capacitance and conductivity. Like stems, leaves of more anisohydric species had lower hydraulic conductance; however, unlike stems, their leaves had higher native capacitance at their daily minimum values of leaf Ψ. Moreover, rates of VPD-induced stomatal closure were related to intrinsic rather than native leaf capacitance and were not associated with species' degree of iso/anisohydry. Our results suggest a trade-off between hydraulic storage and efficiency in the leaf, but a coordination between hydraulic storage and efficiency in the stem along a spectrum of plant iso/anisohydry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pce.13543DOI Listing
July 2019

Polypeptides extracted from Eupolyphaga sinensis walker via enzymic digestion alleviate UV radiation-induced skin photoaging.

Biomed Pharmacother 2019 Apr 22;112:108636. Epub 2019 Feb 22.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, PR China; HeNan Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, HeNan Province, Zhengzhou, 450001, Henan, PR China; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, HeNan Province, Zhengzhou, 450001, Henan, PR China. Electronic address:

The traditional medicine-Eupolyphaga sinensis walker is a rich source of functional proteins and peptides. In this study, Eupolyphaga sinensis walker were identified to include fifteen amino acids, of which hydrophobic amino acids accounted for 46.7%. Eupolyphaga sinensis walker polypeptides (EPs) were extracted by sequentially hydrolyzing with pepsin and trypsin. EPs could effectively scavenge 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·), superoxide anion radical (O), and hydroxyl radical (OH-), and reduce ferric solution in vitro. EPs also enhanced the activities of Ssuperoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPH-Px), increased the contents of hydroxyproline (HYP), and reduced the content of Malondialdehyde (MDA) in photoaged mice skin. Histological study confirmed that EPs improved UV irradiation-induced damage of skin texture and morphology. Therefore, the extracted EPs are effective antioxidants and can serve as powerful treatment for skin photoaging.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2019.108636DOI Listing
April 2019

Unaltered phenology but increased production of ectomycorrhizal roots of Pinus elliottii under 4 years of nitrogen addition.

New Phytol 2019 03 15;221(4):2228-2238. Epub 2018 Nov 15.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Timing (phenology) and amount (production) are two integral facets of root growth, and their shifts have profound influences on below-ground resource acquisition. However, the environmental control and the effects of nitrogen (N) deposition on the production and phenology of ectomycorrhizal (ECM) roots remain unclear. Using a 4 yr minirhizotron experiment, we explored the control of the production and three phenophases (initiation, peak, and cessation of growth) of ECM roots in two soil layers and investigated their dynamic responses to N addition in a seasonally dry subtropical Pinus elliottii forest. We found a stronger control of water availability on the production and a stronger control of temperature on the phenology of ECM roots under ambient conditions. Temperature was correlated positively with initiation and negatively with cessation, especially in the shallow layer. N addition did not affect the phenology of ECM roots but increased their production by modifying N and phosphorus (P) stoichiometry in the soil and foliage. Our findings suggest a greater sensitivity of production than phenology of ECM roots to N addition. The increased production of ECM roots under N addition could be driven by N-induced P limitation or some combination of below-ground resources (P, N, water).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.15542DOI Listing
March 2019

Metrics and proxies for stringency of regulation of plant water status (iso/anisohydry): a global data set reveals coordination and trade-offs among water transport traits.

Tree Physiol 2019 01;39(1):122-134

USDA Forest Service, Pacific Northwest Research Station, 3200 SW Jefferson Way, Corvallis, OR, USA.

Plants operate along a continuum of stringency of regulation of plant water potential from isohydry to anisohydry. However, most metrics and proxies of plant iso/anisohydric behavior have been developed from limited sets of site-specific experiments. Understanding the underlying mechanisms that determine species' operating ranges along this continuum, independent of site and growing conditions, remains challenging. We compiled a global database to assess the global patterns of metrics and proxies of plant iso/anisohydry and then explored some of the underlying functional traits and trade-offs associated with stringency of regulation that determines where species operate along the continuum. Our results showed that arid and semi-arid biomes were associated with greater anisohydry than more mesic biomes, and angiosperms showed marginally greater anisohydry than gymnosperms. Leaf water potential at the turgor loss point (Ψtlp) and wood density were the two most powerful proxies for ranking the degree of plant iso/anisohydry for a wide range of species and biomes. Both of these simple traits can be easily and rapidly determined, and therefore show promise for a priori mapping and understanding of the global distribution pattern of the degree of plant iso/anisohydry. Generally, the most anisohydric species had the most negative values of Ψtlp and highest wood density, greatest resistance to embolism, lowest hydraulic capacitance and lowest leaf-specific hydraulic conductivity of their branches. Wood density in particular appeared to be central to a coordinated series of traits, trade-offs and behaviors along a continuum of iso/anisohydry. Quantification of species' operating ranges along a continuum of iso/anisohydry and identification of associated trade-offs among functional traits may hold promise for mechanistic modeling of species-specific responses to the anticipated more frequent and severe droughts under global climate change scenarios.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/treephys/tpy087DOI Listing
January 2019

Neck circumference may be a valuable tool for screening individuals with obesity: findings from a young Chinese population and a meta-analysis.

BMC Public Health 2018 04 20;18(1):529. Epub 2018 Apr 20.

College of Public Health, Zhengzhou University, No. 100 of Science Avenue, Zhengzhou, 450001, Henan, China.

Background: Central obesity and overweight/obesity can result in various chronic non-communicable diseases, such as cardiovascular disease, metabolic syndrome, and diabetes mellitus. Waist circumference (WC) and body mass index (BMI) are widely used to measure obesity despite their limitations. For example, WC and BMI cannot be measured in pregnant women and subjects with abdominal ascites or masses. Therefore, this study aims to determine the efficacy of neck circumference (NC) as a tool for screening central obesity and overweight/obesity.

Methods: A total of 1169 undergraduates aged 18-25 years were studied by a cross-sectional survey in China, 2016. Questionnaires and physical examinations were used to collect data. Receiver operator characteristic (ROC) curve was performed to determine the best threshold of NC for screening central obesity and overweight/obesity. Meanwhile, a meta-analysis was conducted to estimate the efficacy of NC for screening central obesity and overweight/obesity synthetically.

Results: NC was moderately correlated with WC and BMI. The ROC analysis showed that 37.1 cm for male and 32.6 cm for female were the best thresholds for central obesity, and 37.4 cm and 32.2 cm for overweight/obesity, respectively. The sensitivity, specificity, area under receiver operating curve (AUC) of central obesity and overweight/obesity were higher. In the meta-analysis, the pooled sensitivity, specificity, AUC and their 95%CI of NC for screening central obesity were 0.72 (0.68~ 0.75), 0.87 (0.74~ 0.94), 0.77 (0.73~ 0.80) for male and 0.73 (0.65~ 0.80), 0.80 (0.71~ 0.86), 0.82 (0.79~ 0.86) for female. For overweight/obesity, the pooled sensitivity, specificity, AUC and corresponding 95%CI were 0.83 (0.70~ 0.91), 0.77 (0.66~ 0.85), 0.86 (0.83~ 0.89) for male and 0.82 (0.71~ 0.90), 0.84 (0.61~ 0.95), 0.89 (0.86~ 0.92) for female.

Conclusion: NC may not be a good tool for screening individuals with central obesity. But it may be a simple and valuable tool for screening individuals with overweight/obesity, especially in females.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12889-018-5448-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5910608PMC
April 2018

Nitrogen deposition increases root production and turnover but slows root decomposition in Pinus elliottii plantations.

New Phytol 2018 06 7;218(4):1450-1461. Epub 2018 Mar 7.

Qianyanzhou Ecological Research Station, Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China.

Fine roots of woody plants comprise multiple root orders, which can be functionally partitioned into two pools: absorptive fine roots (AFRs, orders 1, 2) and transport fine roots (TFRs, orders 3-5). However, the function-based fine-root dynamics and especially their responses to increased nitrogen (N) availability remain unclear. We explored dynamic responses of both AFRs and TFRs of Pinus elliottii to N addition in subtropical China based on a 4-yr minirhizotron experiment and a two-stage - early (0.5 yr) vs late (4 yr) - decomposition experiment. N addition increased the production, mortality, and turnover of AFRs but not TFRs. High rates of N persistently inhibited AFR decomposition but affected TFR decomposition differentially at the early (no effect) and late (negative effect) stages. The increased production of AFRs was driven by N-induced decrease in foliar and soil phosphorus (P) concentrations. The decreased decomposition of AFRs might be due to the increased acid-unhydrolyzable residues in decomposing roots. AFRs are the resource-acquiring module, the increased carbon allocation to AFRs may represent a P-acquiring strategy when N no longer limits growth of P. elliottii. Our results suggest that AFRs and TFRs respond differently to N deposition, both in terms of production, mortality, and turnover and in terms of decomposition.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/nph.15066DOI Listing
June 2018

The polymorphism of rs266729 in adiponectin gene and type 2 diabetes mellitus: A Meta-Analysis.

Medicine (Baltimore) 2017 Nov;96(47):e8745

Department of Biostatistics and Epidemiology, College of Public Health Department of Histology and Embryology, Basic Medical College, Zhengzhou University, Zhengzhou, Henan, China.

Genome-wide association studies and meta-analyses indicate that the polymorphism of rs266729 in adiponectin gene increases the risk of type 2 diabetes mellitus (T2DM); however, these study methods have not been able to identify the underlying genetic effect on the development of T2DM. A genetic model-free approach was conducted to determine the underlying genetic model of inheritance of T2DM because of rs266729 in adiponectin gene.We searched available studies on the association between the rs266729 in adiponectin gene and T2DM in accordance with the inclusion and exclusion criteria. Based on the information extracted from the studies, generalized odds ratio value (GOR) was used to evaluate whether the rs266729 polymorphism was a risk factor for T2DM. The parameter λ was calculated to estimate the genetic model, which was defined as the quotient of natural logarithm odds ratio of GC to CC divided by the natural logarithm odds ratio of GG to CC. Finally, binary logistic regression analysis was used to calculate the genetic effect of rs266729 on T2DM.Data from 7 studies were included in this meta-analysis. The total number of subjects was 12,323, comprising 5,948 cases and 6,395 controls. Mean (standard deviation) age of cases was 59.50 (11.53), and that of the controls was 53.80 (11.65), whereas the proportion of male was 40.9 and 50.0%, respectively. GOR was 1.13 (1.02, 1.25) and λ was 0.47 (0.29, 0.64). The result of logistic regression indicated that the G allele influenced the development of T2DM in the additive model, whereas the genetic effect was 1.13 (1.06, 1.19). Sources of control populations were the cause of between-study heterogeneity; nonetheless, there was no publication bias among studies.The G allele of rs266729 in adiponectin gene increases the risk of T2DM through an additive genetic model with an effect of 1.13 (1.06, 1.19).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000008745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708967PMC
November 2017
-->