Publications by authors named "Xiaolei Wang"

590 Publications

ZnO/CPAN Modified Contact Lens with Antibacterial and Harmful Light Reduction Capabilities.

Adv Healthc Mater 2021 Apr 19:e2100259. Epub 2021 Apr 19.

College of Chemistry, Nanchang University, Nanchang, Jiangxi, 330088, China.

Compared with traditional glasses, the comfortable and convenient contact lens (CL) has seen an upsurge among the public. However, due to the lack of antibacterial properties of ordinary CLs, the risk of eye infection is greatly increased accordingly. On the other hand, ordinary CLs also cannot effectively reduce the short-wavelength blue light emitted from electronic products, such as mobile phones and computers. Aiming at the above two problems, zinc oxide (ZnO)/cyclized polyacrylonitrile (CPAN) composites are developed for CL modification. After loading with ZnO/CPAN (ZC), the CL shows a broad-spectrum antibacterial property. Further experiments also prove that it can block UVB, UVA, as well as blue light selectively, under the premise of ensuring hydrophilicity and certain transparency. Theoretically, this ZC-decorated CL can fundamentally reduce the damage to the eyes from harmful light emitted by light-emitting diodes and the secretion of pro-inflammatory factors, which is thus a promising eye protection strategy for modern society.
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http://dx.doi.org/10.1002/adhm.202100259DOI Listing
April 2021

Multifunctional Sensing Based on an Ultrathin Transferrable Microring Laser.

ACS Appl Mater Interfaces 2021 Apr 16. Epub 2021 Apr 16.

College of Physics and Optoelectronics, Faculty of Science, Beijing University of Technology, Beijing 100124, China.

An ultrathin-film microring laser was fabricated using inkjet printing and a simple lift-off technique. Whispering-gallery-mode lasing was observed under optically pumped conditions in the film. The freestanding laser can be transferred to arbitrary surfaces for multifunctional applications, such as acoustic and relative humidity sensing. Using the first eigenmode of a membrane vibration, an acoustic sensor with a 0.15 Pa limit of detection was demonstrated via laser bandwidth broadening. A relative humidity sensor with a 1.1% limit of detection via wavelength shifts was demonstrated by placing the device on an optical fiber facet. These cost-effective, transferrable, multifunctional laser sensors will have many additional applications.
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http://dx.doi.org/10.1021/acsami.1c03123DOI Listing
April 2021

Myocardial protective effect of intracoronary administration of nicorandil and alprostadil via targeted perfusion microcatheter in patients undergoing elective percutaneous coronary intervention: A randomized controlled trial.

Medicine (Baltimore) 2021 Apr;100(15):e25551

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University.

Background: The aim of the study was to evaluate the efficacy of nicorandil and alprostadil on myocardial protection in patients undergoing elective percutaneous coronary intervention (PCI).

Methods: In this prospective, single-blinded, randomized controlled study, 90 consecutive patients scheduled for elective PCI for de novo coronary lesions were assigned to the nicorandil, alprostadil, and nitroglycerin groups in a 1:1:1 ratio. Drugs were administered intracoronary via a targeted perfusion microcatheter. The primary endpoint was the thrombolysis in myocardial infarction (TIMI) myocardial perfusion frame count (TMPFC). Additionally, the corrected TIMI frame count (cTFC), TIMI myocardial perfusion grade (TMPG), and incidence of periprocedural myocardial injury (PMI) were assessed.

Results: Both nicorandil and alprostadil were significantly effective in reducing TMPFC (114.6 ± 33.7 vs 93.4 ± 30.9, P = .016; 114.3 ± 34.3 vs 94.7 ± 33.3, P = .029, respectively). Similar findings were observed in the improvement of cTFC (20.3 ± 10.5 vs 13.5 ± 5.0, P = .003; 20.2 ± 7.4 vs 15.2 ± 5.2, P = .003, respectively) and percentage of TMPG 3 (100% vs 82.8%, P = .052; 83.3% vs 96.7%, P = .196, respectively); whereas, nitroglycerin produced a limited effect on TMPFC (114.4 ± 30.9 vs 112.1 ± 31.9, P = .739), cTFC (19.4 ± 7.2 vs 19.3 ± 7.2, P = .936), and percentage of TMPG 3 (86.7% vs 86.7%, P = 1.000). No significant difference was found in the incidence of PMI (16.7% vs 16.0% vs 27.6%, P = .537), though it was comparatively lower in the nicorandil and alprostadil groups. Furthermore, the intracoronary administration of nicorandil and alprostadil had a mild effect on blood pressure and heart rate.

Conclusions: The intracoronary administration of nicorandil and alprostadil via a targeted perfusion microcatheter was more effective in improving myocardial perfusion in patients undergoing elective PCI than nitroglycerin.
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http://dx.doi.org/10.1097/MD.0000000000025551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052047PMC
April 2021

Improving the performance of proteomic analysis via VAILase cleavage and 193-nm ultraviolet photodissociation.

Anal Chim Acta 2021 Apr 20;1155:338340. Epub 2021 Feb 20.

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

Further improving the proteomic identification coverage and reliability is still challenging in the mass spectrometry (MS)-based proteomics. Herein, we combine VAILase and trypsin digestion with 193-nm ultraviolet photodissociation (UVPD) and higher-energy collision dissociation (HCD) to improve the performance of bottom-up proteomics. As VAILase exhibits high complementarity to trypsin, the proteome sequence coverage is improved obviously whether with HCD or 193-nm UVPD. The high diversity of fragment ion types produced by UVPD contributes to the improvements of identification reliability for both trypsin- and VAILase-digested peptides with an average XCorr score improvement of 10%.
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http://dx.doi.org/10.1016/j.aca.2021.338340DOI Listing
April 2021

Inhibition of miRNA-155 Alleviates High Glucose-Induced Podocyte Inflammation by Targeting SIRT1 in Diabetic Mice.

J Diabetes Res 2021 8;2021:5597394. Epub 2021 Mar 8.

Department of Endocrinology, Dongfang Hospital, Beijing University of Chinese Medicine, 6 Fangxingyuan, Fengtai District, Beijing, China.

Objective: Microinflammation plays a crucial role in podocyte dysfunction in diabetic nephropathy, but its regulatory mechanism is still unclear. This study is aimed at discussing the mechanisms underlying the effect of miRNA-155 on podocyte injury to determine its potential as a therapeutic target.

Methods: Cultured immortalized mouse podocytes and diabetic KK-Ay mice models were treated with a miR-155 inhibitor. Western blotting, real-time PCR, ELISA, immunofluorescence, and Luciferase reporter assay were used to analyze markers of inflammation cytokines and podocyte injury.

Results: miRNA-155 was found to be highly expressed in serum and kidney tissue of mice with diabetic nephropathy and in cultured podocytes, accompanied by elevated levels of inflammatory factors. Inhibition of miRNA-155 can reduce proteinuria and ACR levels, diminish the secretion of inflammatory molecules, improve kidney function, inhibit podocyte foot fusion, and reverse renal pathological changes in diabetic nephropathy mice. Overexpression of miRNA-155 can increase inflammatory molecule production in podocytes and aggravates podocyte injury, while miRNA-155 inhibition suppresses inflammatory molecule production in podocytes and reduces podocyte injury. A luciferase assay confirmed that miRNA-155 could selectively bind to 3'-UTR of SIRT1, resulting in decreased SIRT1 expression. In addition, SIRT1 siRNA could offset SIRT1 upregulation and enhance inflammatory factor secretion in podocytes, induced by the miRNA-155 inhibitor.

Conclusions: These findings strongly support the hypothesis that miRNA-155 inhibits podocyte inflammation and reduces podocyte injury through SIRT1 silencing. miRNA-155 suppression therapy may be useful for the management of diabetic nephropathy.
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http://dx.doi.org/10.1155/2021/5597394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7960039PMC
March 2021

Enlightenment from the COVID-19 Pandemic: The Roles of Environmental Factors in Future Public Health Emergency Response.

Engineering (Beijing) 2021 Mar 13. Epub 2021 Mar 13.

Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, SK, S7N 5E2, Canada.

The coronavirus disease 2019 (COVID-19) pandemic is challenging the current public health emergency response systems (PHERSs) of many countries. Although environmental factors, such as those influencing the survival of viruses and their transmission between species including humans, play important roles in PHERSs, little attention has been given to these factors. This study describes and elucidates the roles of environmental factors in future PHERSs. To improve countries' capability to respond to public health emergencies associated with viral infections such as the COVID-19 pandemic, a number of environmental factors should be considered before, during, and after the responses to such emergencies. More specifically, to prevent pandemic outbreaks, we should strengthen environmental and wildlife protection, conduct detailed viral surveillance in animals and hotspots, and improve early-warning systems. During the pandemic, we must study the impacts of environmental factors on viral behaviors, develop control measures to minimize secondary environmental risks, and conduct timely assessments of viral risks and secondary environmental effects with a view to reducing the impacts of the pandemic on human health and on ecosystems. After the pandemic, we should further strengthen surveillance for viruses and the prevention of viral spread, maintain control measures for minimizing secondary environmental risks, develop our capability to scientifically predict pandemics and resurgences, and prepare for the next unexpected resurgence. Meanwhile, we should restore the normal life and production of the public based on the "One Health" concept, that views global human and environmental health as inextricably linked. Our recommendations are essential for improving nations' capability to respond to global public health emergencies.
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http://dx.doi.org/10.1016/j.eng.2020.12.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955573PMC
March 2021

Immunopathogenesis in HIV-associated pediatric tuberculosis.

Pediatr Res 2021 Mar 17. Epub 2021 Mar 17.

Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University School of Medicine, Covington, LA, USA.

Tuberculosis (TB) is an increasing global emergency in human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) patients, in which host immunity is dysregulated and compromised. However, the pathogenesis and efficacy of therapeutic strategies in HIV-associated TB in developing infants are essentially lacking. Bacillus Calmette-Guerin vaccine, an attenuated live strain of Mycobacterium bovis, is not adequately effective, which confers partial protection against Mycobacterium tuberculosis (Mtb) in infants when administered at birth. However, pediatric HIV infection is most devastating in the disease progression of TB. It remains challenging whether early antiretroviral therapy (ART) could maintain immune development and function, and restore Mtb-specific immune function in HIV-associated TB in children. A better understanding of the immunopathogenesis in HIV-associated pediatric Mtb infection is essential to provide more effective interventions, reducing the risk of morbidity and mortality in HIV-associated Mtb infection in infants. IMPACT: Children living with HIV are more likely prone to opportunistic infection, predisposing high risk of TB diseases. HIV and Mtb coinfection in infants may synergistically accelerate disease progression. Early ART may probably induce immune reconstitution inflammatory syndrome and TB pathology in HIV/Mtb coinfected infants.
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http://dx.doi.org/10.1038/s41390-021-01393-xDOI Listing
March 2021

MiR-1290 promotes myoblast differentiation and protects against myotube atrophy via Akt/p70/FoxO3 pathway regulation.

Skelet Muscle 2021 Mar 15;11(1). Epub 2021 Mar 15.

Department of Pain, Huadong Hospital, Shanghai Key Laboratory of Clinical Geriatric Medicine, Fudan University, No. 221, West YanAn Rd, Shanghai, 200040, P.R. China.

Background: Sarcopenia is a common skeletal disease related to myogenic disorders and muscle atrophy. Current clinical management has limited effectiveness. We sought to investigate the role of miR-1290 in myoblast differentiation and muscle atrophy.

Methods: By transfecting miR-1290 into C2C12 cells, we investigated whether miR-1290 regulates myogenesis and myotube atrophy via AKT/P70 signaling pathway. MHC staining was performed to assess myoblast differentiation. Differentiation-related MHC, Myod, and Myog protein levels, and atrophy-related MuRF1 and atrogin-1 were explored by western blot. An LPS-induced muscle atrophy rat model was developed. RT-PCR was conducted to analyze miR-1290 serum levels in muscle atrophy patients and normal controls (NCs).

Results: The miR-1290 transfection increased MHC-positive cells and MHC, Myod, and Myog protein levels in the miR-1290 transfection group, demonstrating that miR-1290 promoted C2C12 myoblast differentiation. Myotube diameter in the miR-1290 transfection group was higher than in the TNF-α-induced model group. Western blot analysis showed decreased MuRF1 and atrogin-1 levels in the miR-1290 transfection group compared with the model group, demonstrating that miR-1290 protected against myoblast cellular atrophy. Luciferase assay and western blot analysis showed that miR-1290 regulation was likely caused by AKT/p70/FOXO3 phosphorylation activation. In the LPS-induced muscle atrophy rat model, miR-1290 mimics ameliorated gastrocnemius muscle loss and increased muscle fiber cross-sectional area. Clinically, miR-1290 serum level was significantly decreased in muscle atrophy patients.

Conclusions: We found that miR-1290 enhances myoblast differentiation and inhibits myotube atrophy through Akt/p70/FoxO3 signaling in vitro and in vivo. In addition, miR-1290 may be a potential therapeutic target for sarcopenia treatment.
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http://dx.doi.org/10.1186/s13395-021-00262-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958887PMC
March 2021

Regulation of Blood Glucose Using Islets Encapsulated in a Melanin-Modified Immune-Shielding Hydrogel.

ACS Appl Mater Interfaces 2021 Mar 10;13(11):12877-12887. Epub 2021 Mar 10.

The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330088, P. R. China.

Islet transplantation is currently a promising treatment for type 1 diabetes mellitus. However, the foreign body reaction and retrieval difficulty often lead to transplantation failure and hinder the clinical application. To address these two challenges, we propose a balanced charged sodium alginate-polyethyleneimine-melanin (SA-PEI-Melanin) threadlike hydrogel with immune shielding and retrievable properties. The attractiveness of this study lies in that the introduction of melanin can stimulate insulin secretion, especially under near-infrared (NIR) irradiation. After demonstrating a good immune-shielding effect, we performed an transplantation experiment. The results showed that the blood glucose level in the SA-PEI-Melanin group was stably controlled below the diabetic blood glucose criterion, and this blood glucose level could be further adjusted after NIR irradiation. In addition, the evaluation after retrieving the SA-PEI-Melanin hydrogel indicated that the islets still maintained a normal physiological function, further proving its excellent immunological protection. This study provides a new approach for the accurate regulation of blood glucose in patients with type 1 diabetes mellitus and contributes to developing a promising transplant system to reconcile real-time and precise light-defined insulin secretion regulation.
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http://dx.doi.org/10.1021/acsami.0c23010DOI Listing
March 2021

Residual Proviral Reservoirs: A High Risk for HIV Persistence and Driving Forces for Viral Rebound after Analytical Treatment Interruption.

Viruses 2021 02 21;13(2). Epub 2021 Feb 21.

Tulane National Primate Research Center, Division of Comparative Pathology, Tulane University School of Medicine, 18703 Three Rivers Road, Covington, LA 70433, USA.

Antiretroviral therapy (ART) has dramatically suppressed human immunodeficiency virus (HIV) replication and become undetectable viremia. However, a small number of residual replication-competent HIV proviruses can still persist in a latent state even with lifelong ART, fueling viral rebound in HIV-infected patient subjects after treatment interruption. Therefore, the proviral reservoirs distributed in tissues in the body represent a major obstacle to a cure for HIV infection. Given unavailable HIV vaccine and a failure to eradicate HIV proviral reservoirs by current treatment, it is crucial to develop new therapeutic strategies to eliminate proviral reservoirs for ART-free HIV remission (functional cure), including a sterilizing cure (eradication of HIV reservoirs). This review highlights recent advances in the establishment and persistence of HIV proviral reservoirs, their detection, and potential eradication strategies.
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http://dx.doi.org/10.3390/v13020335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926539PMC
February 2021

Wicking-Polarization-Induced Water Cluster Size Effect on Triboelectric Evaporation Textiles.

Adv Mater 2021 Apr 3;33(15):e2007352. Epub 2021 Mar 3.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, 201620, P. R. China.

Sweating during exercise, physical labor, or hot weather leads to a feeling of discomfort. The stuffiness, stickiness, and heaviness brought by sweat may promote negative emotions or disease. Clothing, textiles, and wearable devices exacerbate these problems by restricting evaporation of sweat. Here, a textile that can promote and enhance sweat evaporation by coupling wicking and polarization is reported. The wicking is produced by the wettability gradient and pore size, which make the surface moisture content of the textile in contact with the skin strictly 0%. The polarization is driven by a ferroelectric-enhanced triboelectric textile. This textile degrades large-sized water clusters into small-sized water clusters or water monomers, so that the textiles have an excellent moisture evaporation rate (4.4 and 3.6 times faster than the cotton and polyester textiles, respectively). This work provides a new source of inspiration for quick-drying textiles and also finds an attractive application for triboelectric technology.
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http://dx.doi.org/10.1002/adma.202007352DOI Listing
April 2021

Risk factors for brain metastases from non-small-cell lung cancer: A protocol for observational study.

Medicine (Baltimore) 2021 Mar;100(9):e24724

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China.

Abstract: Brain metastasis is a common site of distant metastasis of non-small-cell lung cancer (NSCLC) that greatly reduces the prognosis of patients. In this study, we explored the correlation between different clinical factors and secondary brain metastases in NSCLC in an attempt to identify NSCLC patient populations at high risk of metastasis to the central nervous system.We collected data for 350 NSCLC patients from the medical record system of the First Affiliated Hospital of Nanchang University from June 2015 to June 2019, and these patients had pathologically verified diagnoses. The correlations between age at the time of diagnosis, sex, histological type, calcium concentration, hemoglobin (HB), fibrinogen (Fbg), activated partial thromboplastin time (APTT), alkaline phosphatase (ALP), carcinoembryonic antigen (CEA), CA125, and CA199 levels and brain metastasis were analyzed. Multivariate logistic regression analysis was used to identify risk factors for NSCLC brain metastasis. A receiver operating characteristic (ROC) curve was used to calculate the cutoff, sensitivity, and specificity of the independent related factors.Of the 350 patients, 57 were diagnosed with brain metastases. Univariate and multivariate logistic regression analysis indicated that lesion diameter, calcium concentration, and CEA level were independent risk factors correlated with brain metastasis (P < .05). There were no significant differences in age, sex, type of histopathology, presence or absence of mediastinal lymph node metastasis, HB, Fbg, APTT, ALP, cancer antigen 125 (CA-125), or cancer antigen 199 (CA-199) levels between patients with brain metastases and patients without brain metastases (P > .05, respectively). ROC curves demonstrated that these factors had comparable accuracy in predicting brain metastasis (area under the curve [AUCs] were 0.620, 0.661, and 0.729, respectively). The cutoff values for lesion diameter, calcium, and CEA were 5.050 cm, 2.295 mmol/L, and 11.160 ng/mL, respectively. The sensitivities for prediction brain metastasis were 59.6%, 64.9%, and 73.3%, with specificities of 63.1%, 59.2%, and 70.3%, respectively.According to our study, lesion diameter, calcium concentration, and CEA level are independent risk factors for brain metastases in NSCLC patients. Thus, we can strengthen the regular follow-up of NSCLC patients with tumor diameter > 5.050 cm, calcium > 2.295 mmol/L, CEA > 11.160 ng/mL, and use these factors as a reference for preventive treatments.
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http://dx.doi.org/10.1097/MD.0000000000024724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7939174PMC
March 2021

Discovery of A031 as effective proteolysis targeting chimera (PROTAC) androgen receptor (AR) degrader for the treatment of prostate cancer.

Eur J Med Chem 2021 Apr 23;216:113307. Epub 2021 Feb 23.

State Key Laboratory of Applied Organic Chemistry, Department of Chemistry, Lanzhou University, 222 S. Tianshui Rd, Lanzhou, 730000, PR China. Electronic address:

Androgen receptor (AR) is an effective therapeutic target for the treatment of prostate cancer. We report herein the design, synthesis, and biological evaluation of highly effective proteolysis targeting chimeras (PROTAC) androgen receptor (AR) degraders, such as compound A031. It could induce the degradation of AR protein in VCaP cell lines in a time-dependent manner, achieving the IC 50 value of less than 0.25 μM. The A031 is 5 times less toxic than EZLA and works with an appropriate half-life (t 1/2) or clearance rate (Cl). Also, it has a significant inhibitory effect on tumor growth in zebrafish transplanted with human prostate cancer (VCaP). Therefore, A031 provides a further idea of developing novel drugs for prostate cancer.
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http://dx.doi.org/10.1016/j.ejmech.2021.113307DOI Listing
April 2021

Serum Metabolites as an Indicator of Developing Gestational Diabetes Mellitus Later in the Pregnancy: A Prospective Cohort of a Chinese Population.

J Diabetes Res 2021 5;2021:8885954. Epub 2021 Feb 5.

Xiangya School of Public Health, Central South University, Changsha, China.

Objective: Gestational diabetes mellitus (GDM) is a common metabolic disorder with onset during pregnancy. However, the etiology and pathogenesis of GDM have not been fully elucidated. In this study, we used a metabolomics approach to investigate the relationship between maternal serum metabolites and GDM in early pregnancy.

Methods: A nested case-control study was performed. To establish an early pregnancy cohort, pregnant women in early pregnancy (10-13 weeks) were recruited. In total, 51 patients with GDM and 51 healthy controls were included. Serum samples were analyzed using an untargeted high-performance liquid chromatography mass spectrometry metabolomics approach. The relationships between metabolites and GDM were analyzed by an orthogonal partial least-squares discriminant analysis. Differential metabolites were evaluated using a KEGG pathway analysis.

Results: A total of 44 differential metabolites were identified between GDM cases and healthy controls during early pregnancy. Of these, 26 significant metabolites were obtained in early pregnancy after false discovery rate (FDR < 0.1) correction. In the GDM group, the levels of L-pyroglutamic acid, L-glutamic acid, phenylacetic acid, pantothenic acid, and xanthine were significantly higher and the levels of 1,5-anhydro-D-glucitol, calcitriol, and 4-oxoproline were significantly lower than those in the control group. These metabolites were involved in multiple metabolic pathways, including those for amino acid, carbohydrate, lipid, energy, nucleotide, cofactor, and vitamin metabolism.

Conclusions: We identified significant differentially expressed metabolites associated with the risk of GDM, providing insight into the mechanisms underlying GDM in early pregnancy and candidate predictive markers.
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http://dx.doi.org/10.1155/2021/8885954DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884125PMC
February 2021

Anemia among Chinese patients with chronic kidney disease and its association with quality of life - results from the Chinese cohort study of chronic kidney disease (C-STRIDE).

BMC Nephrol 2021 Feb 22;22(1):64. Epub 2021 Feb 22.

Department of Nephrology, Guizhou Provincial People's Hospital, Guizhou University School of medicine, Gui Yang, China.

Background: Anemia is one of the common complications in patients with chronic kidney disease (CKD). However, there is no systematic investigation on the prevalence of anemia in CKD patients and its relationship with the quality of life in China.

Methods: The data for this study comes from baseline data from the Chinese Chronic Kidney Disease Cohort Study (C-STRIDE), which recruited predialysis CKD patients in China. The kidney disease quality of life summary (KDQOL-TM) was used to assess health-related quality of life (HRQoL). Use linear regression model to estimate the relationship between hemoglobin level and quality of life.

Results: A total of 2921 patients were included in this study. The adjusted prevalence of hemoglobin (Hb) less than 100 g/L was 10.3% (95% confidence interval [CI]: 9.9,11.4%), and showed an increased trend through reduced eGFR levels from 4.0% (95%CI:2.3,5.9%) in the 45-60 ml/min/1.73m group to 23.4% (95%CI:20.5,26.2%) in the 15-29 ml/min/1.73m group. The prevalence of anti-anemia treatment was 34.0% (95%CI: 28.7,39.3%) and it is shown by reducing eGFR levels from 15.8% (95%CI:0,36.7%) in the 45-60 ml/min/1.73m group to 38.2% (95%CI: 30.7,45.2%) in the 15-29 ml/min/1.73m group. All five dimensions of the KDQOL scores in patients with CKD decreased as hemoglobin declined. After multivariable adjustments,the degrees of decrease became somewhat blunted. For example, compared with hemoglobin of ≥130 g/L, regression coefficients in the hemoglobin of < 100 g/L were - 0.047(95%CI: - 0.049,-0.045) for Symptoms and Problems(S), - 0.047(95%CI: - 0.049,-0.044) for Effects of the Kidney Disease(E), - 0.207(95%CI: - 0.212,-0.203) for Burden of the Kidney Disease(B), - 0.112(95%CI: - 0.115,-0.109) for SF-12 Physical Functioning (PCS), - 0.295(95%CI: - 0.299, -0.292) for SF-12 Mental Functioning (MCS), respectively.

Conclusions: In our cross-sectional analysis of patients with CKD in China, prevalence of both anemia and anti-anemia treatment increased with decreased eGFR. In addition, anemia was associated with reduced HRQoL.
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http://dx.doi.org/10.1186/s12882-021-02247-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898739PMC
February 2021

Immune Responses and Viral Persistence in Simian/Human Immunodeficiency Virus SHIV.C.CH848-Infected Rhesus Macaques.

J Virol 2021 Apr 12;95(9). Epub 2021 Apr 12.

Tulane National Primate Research Center, Tulane University School of Medicine, Covington, Louisiana, USA

Chimeric simian/human immunodeficiency viruses (SHIVs) are widely used in nonhuman primate models to recapitulate human immunodeficiency virus (HIV) infection in humans, yet most SHIVs fail to establish persistent viral infection. We investigated immunological and virological events in rhesus macaques infected with the newly developed SHIV.C.CH848 (SHIVC) and treated with combined antiretroviral therapy (cART). Similar to HIV/simian immunodeficiency virus (SIV) infection, SHIV.C.CH848 infection established viral reservoirs in CD4 T cells and myeloid cells, accompanied by productive infection and depletion of CD4 T cells in systemic and lymphoid tissues throughout SHIV infection. Despite 6 months of cART-suppressed viral replication, integrated proviral DNA levels remained stable, especially in CD4 T cells, and the viral rebound was also observed after ART interruption. Autologous neutralizing antibodies to the parental HIV-1 strain CH848 were detected, with limited viral evolution at 5 months postinfection. In comparison, heterogenous neutralizing antibodies in SHIV.C.CH848-infected macaques were not detected except for 1 (1 of 10) animal at 2 years postinfection. These findings suggest that SHIV.C.CH848, a novel class of transmitted/founder SHIVs, can establish sustained viremia and viral reservoirs in rhesus macaques with clinical immunodeficiency consequences, providing a valuable SHIV model for HIV research. SHIVs have been extensively used in a nonhuman primate (NHP) model for HIV research. In this study, we investigated viral reservoirs in tissues and immune responses in an NHP model inoculated with newly generated transmitted/founder HIV-1 clade C-based SHIV.C.CH848. The data show that transmitted founder (T/F) SHIVC infection of macaques more closely recapitulates the virological and clinical features of HIV infection, including persistent viremia and viral rebound once antiretroviral therapy is discontinued. These results suggest this CCR5-tropic, SHIVC strain is valuable for testing responses to HIV vaccines and therapeutics.
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http://dx.doi.org/10.1128/JVI.02198-20DOI Listing
April 2021

Oxygen-Functionalized Polyacrylonitrile Nanofibers with Enhanced Performance for Lithium-Ion Storage.

ACS Omega 2021 Feb 15;6(4):2542-2548. Epub 2021 Jan 15.

Key Laboratory of Functional Materials and Devices for Special Environments, Xinjiang Technical Institute of Physics & Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.

Functionalization and morphological construction can promote lithium-ion storage performance of organic polymers. In this contribution, exceptional lithium ion storage performance is empowered to porous polyacrylonitrile (PAN) nanofibers via the integration of template-assisted electrospinning technology and thermal treatment. It is found that the atmosphere adopted during the annealing process controls the storage behaviors of Li. Impressively, the samples annealed in air present competitive capacities, rate capabilities, and a stable lifetime, compared with other counterparts (PAN powders and PAN fibers treated in N). Such enhancement in performance is attributed to the enriched oxygen-based functionalities (mainly C=O group) which guarantee a high specific capacity and the porous structure which facilitates the transportation of Li and electrons to improve the rate capability. It is envisioned that such morphology control and surface functionalization open up new horizons in the development of organic electrode materials with enhanced lithium-ion storage performances.
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http://dx.doi.org/10.1021/acsomega.0c04326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859936PMC
February 2021

NLRP3 inflammasome is a key driver of Obesity-Induced atrial arrhythmias.

Cardiovasc Res 2021 Feb 1. Epub 2021 Feb 1.

Department of Molecular Physiology and Biophysics.

Aims: Obesity, an established risk factor of atrial fibrillation (AF), is frequently associated with enhanced inflammatory response. However, whether inflammatory signaling is causally linked to AF pathogenesis in obesity remains elusive. We recently demonstrated that the constitutive activation of the 'NACHT, LRR & PYD Domains-containing Protein 3' (NLRP3) inflammasome promotes AF susceptibility. In this study, we hypothesized that the NLRP3 inflammasome is a key driver of obesity-induced AF.

Methods And Results: Western blotting was performed to determine the level of NLRP3 inflammasome activation in atrial tissues of obese patients, sheep, and diet-induced obese (DIO) mice. The increased bodyweight in patients, sheep, and mice was associated with enhanced NLRP3-inflammasome activation. To determine whether NLRP3 contributes to the obesity-induced atrial arrhythmogenesis, wildtype (WT) and NLRP3 homozygous knockout (NLRP3-/-) mice were subjected to high-fat diet (HFD) or normal chow (NC) for 10 weeks. Relative to NC-fed WT mice, HFD-fed WT mice were more susceptible to pacing-induced AF with longer AF duration. In contrast, HFD-fed NLRP3-/- mice were resistant to pacing-induced AF. Optical mapping in DIO mice revealed an arrhythmogenic substrate characterized by abbreviated refractoriness and action potential duration (APD), two key determinants of reentry-promoting electrical remodeling. Upregulation of ultra-rapid delayed-rectifier K+-channel (Kv1.5) contributed to the shortening of atrial refractoriness. Increased profibrotic signaling and fibrosis along with abnormal Ca2+ release from sarcoplasmic reticulum (SR) accompanied atrial arrhythmogenesis in DIO mice. Conversely, genetic ablation of Nlrp3 (NLRP3-/-) in HFD-fed mice prevented the increases in Kv1.5 and the evolution of electrical remodeling, the upregulation of profibrotic genes, and abnormal SR Ca2+ release in DIO mice.

Conclusions: These results demonstrate that the atrial NLRP3 inflammasome is a key driver of obesity-induced atrial arrhythmogenesis and establishes a mechanistic link between obesity-induced AF and NLRP3-inflammasome activation.
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http://dx.doi.org/10.1093/cvr/cvab024DOI Listing
February 2021

Spatial-frequency encoded imaging of multangular and multispectral images.

Rev Sci Instrum 2021 Jan;92(1):015111

Key Laboratory of Education Ministry for Power Machinery and Engineering, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Modern imaging techniques increasingly require signals to be collected from multiple viewpoints and spectral bands to realize multi-dimensional and multi-species detections. For this purpose, multiple cameras are commonly required. Each camera collects signals from one viewpoint or one spectral band, resulting in a considerable experimental cost. Based on frequency modulation, this work proposes an encoded-imaging technique that can record multangular and multispectral images in one acquisition. The signals recorded from different viewpoints and spectral bands are superimposed in the spatial domain, while being separate in the frequency domain. This allows us to extract individual images based on their respective frequency components. In this work, a proof-of-concept experiment was conducted. The high correlation coefficient between the superimposition of the extracted images and a normal superimposed image demonstrates the effectiveness of this technique. In addition, an improved mathematical formulation was proposed to describe the higher spatial-frequency components, which were considered merely to be residual lines in previous studies. The proposed encoded-imaging technique may have potential for multangular and multispectral imaging, which is especially useful for tomographic reconstructions.
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http://dx.doi.org/10.1063/5.0025112DOI Listing
January 2021

The crosstalk between cardiomyocyte calcium and inflammasome signaling pathways in atrial fibrillation.

Pflugers Arch 2021 Mar 28;473(3):389-405. Epub 2021 Jan 28.

Department of Medicine (Section of Cardiovascular Research), Baylor College of Medicine, Houston, TX, USA.

Atrial fibrillation (AF) is the most frequent arrhythmia in adults. The prevalence and incidence of AF is going to increase substantially over the next few decades. Because AF increases the risk of stroke, heart failure, dementia, and others, it severely impacts the quality of life, morbidity, and mortality. Although the pathogenesis of AF is multifaceted and complex, focal ectopic activity and reentry are considered as the fundamental proarrhythmic mechanisms underlying AF development. Over the past 2 decades, large amount of evidence points to the key role of intracellular Ca dysregulation in both initiation and maintenance of AF. More recently, emerging evidence reveal that NLRP3 (NACHT, LRR, PYD domain-containing 3) inflammasome pathway contributes to the substrate of both triggered activity and reentry, ultimately promoting AF. In this article, we review the current state of knowledge on Ca signaling and NLRP3 inflammasome activity in AF. We also discuss the potential crosstalk between these two quintessential contributors to AF promotion.
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http://dx.doi.org/10.1007/s00424-021-02515-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7940593PMC
March 2021

Arbitrary polarization conversion dichroism metasurfaces for all-in-one full Poincaré sphere polarizers.

Light Sci Appl 2021 Jan 27;10(1):24. Epub 2021 Jan 27.

Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Institute of Photonics Technology, Jinan University, 510632, Guangzhou, China.

The control of polarization, an essential property of light, is of broad scientific and technological interest. Polarizers are indispensable optical elements for direct polarization generation. However, arbitrary polarization generation, except that of common linear and circular polarization, relies heavily on bulky optical components such as cascading linear polarizers and waveplates. Here, we present an effective strategy for designing all-in-one full Poincaré sphere polarizers based on perfect arbitrary polarization conversion dichroism and implement it in a monolayer all-dielectric metasurface. This strategy allows preferential transmission and conversion of one polarization state located at an arbitrary position on the Poincaré sphere to its handedness-flipped state while completely blocking its orthogonal state. In contrast to previous methods that were limited to only linear or circular polarization, our method manifests perfect dichroism of nearly 100% in theory and greater than 90% experimentally for arbitrary polarization states. By leveraging this attractive dichroism, our demonstration of the generation of polarization beams located at an arbitrary position on a Poincaré sphere directly from unpolarized light can substantially extend the scope of meta-optics and dramatically promote state-of-the-art nanophotonic devices.
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http://dx.doi.org/10.1038/s41377-021-00468-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841175PMC
January 2021

Dual-Effective Chronic Wounds Management System through a Monoglyceride Binary Blend Matrix Based Thermal-Responsive Phase-Transition Substrate.

Adv Healthc Mater 2021 Jan 26:e2001966. Epub 2021 Jan 26.

College of Chemistry, Nanchang University, Nanchang, Jiangxi, 330088, China.

A new monoglyceride-based heat-sensitive substrate is proposed, for the first time, as a wound management system. First, an appropriate portion of glyceryl monooleate (GMO) and glyceryl monostearate (GMS) is mixed to provide a thermal phase responsive matrix (GMO-GMS, GG). Subsequently, to improve the photothermal responsive and antibacterial performance, silver nanoparticles (Ag) decorated reduced graphene oxide (rGO) nanocomposite (rGO-Ag) is added to the GG matrix to obtain (GG-rGO-Ag). According to the systematic studies from uninfected, infected, and diabetic wound models, by applying near infrared (NIR) laser, the phase change of GG-rGO-Ag can be triggered to release Ag on demand for sterilization. More importantly, this smart GG substrate can also promote the production of vascular endothelial growth factor protein, thus serving as a NIR defined mufti-effective wound management system.
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http://dx.doi.org/10.1002/adhm.202001966DOI Listing
January 2021

Peptidomics Analysis Discloses That Novel Bioactive Peptides Participate in Necrotizing Enterocolitis in a Rat Model.

Biomed Res Int 2020 31;2020:4705149. Epub 2020 Dec 31.

Department of Neonatology, The Affiliated Wuxi Children's Hospital of Nanjing Medical University, Wuxi, 214023 Jiangsu, China.

Necrotizing enterocolitis (NEC) is a common devastating gastrointestinal disease in premature infants, the molecular mechanisms of which have not been fully elucidated. Recently, endogenous peptides have garnered much attention owing to their role in diagnosis and treatment. However, changes in the peptide expression of NEC intestinal tissues remain poorly understood. In the present study, a comparative peptidomics profiling analysis was performed between NEC and control intestinal tissues via liquid chromatography-tandem mass spectrometry (LC-MS). In total, 103 upregulated and 73 downregulated peptides were identified in the intestinal tissues (fold change ≥ 1.5, < 0.05). Bioinformatics analysis revealed that these differentially expressed peptides were significantly associated with NEC pathophysiology, including apoptosis, the TGF- signaling pathway, the Wnt signaling pathway, and the MAPK signaling pathway. Furthermore, two putative peptides could inhibit apoptosis and promote the migration of intestinal epithelial cells induced by lipopolysaccharide; these peptides were derived from the protein domains MT1 and EZRI, respectively. In conclusion, our study revealed that endogenous peptides are involved in the pathophysiologic mechanism of NEC; nevertheless, further exploration is required in this regard.
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http://dx.doi.org/10.1155/2020/4705149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7790586PMC
December 2020

Mucosal integrin α4β7 blockade fails to reduce the seeding and size of viral reservoirs in SIV-infected rhesus macaques.

FASEB J 2021 Feb;35(2):e21282

Tulane National Primate Research Center, Tulane University School of Medicine, Covington, LA, USA.

Cellular viral reservoirs are rapidly established in tissues upon HIV-1/SIV infection, which persist throughout viral infection, even under long-term antiretroviral therapy (ART). Specific integrins are involved in the homing of cells to gut-associated lymphoid tissues (GALT) and inflamed tissues, which may promote the seeding and dissemination of HIV-1/SIV to these tissue sites. In this study, we investigated the efficacy of prophylactic integrin blockade (α4β7 antibody or α4β7/α4β1 dual antagonist TR-14035) on viral infection, as well as dissemination and seeding of viral reservoirs in systemic and lymphoid compartments post-SIV inoculation. The results showed that blockade of α4β7/α4β1 did not decrease viral infection, replication, or reduce viral reservoir size in tissues of rhesus macaques after SIV infection, as indicated by equivalent levels of plasma viremia and cell-associated SIV RNA/DNA to controls. Surprisingly, TR-14035 administration in acute SIV infection resulted in consistently higher viremia and more rapid disease progression. These findings suggest that integrin blockade alone fails to effectively control viral infection, replication, dissemination, and reservoir establishment in HIV-1/SIV infection. The use of integrin blockade for prevention or/and therapeutic strategies requires further investigation.
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http://dx.doi.org/10.1096/fj.202002235RDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839271PMC
February 2021

Robust anomalous Hall effect and temperature-driven Lifshitz transition in Weyl semimetal MnGe.

Nanoscale 2021 Feb;13(4):2601-2608

State Key Laboratory of Superlattices and Microstructures, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China.

Topological Weyl semimetals have attracted considerable interest because they manifest underlying physics and device potential in spintronics. Large anomalous Hall effect (AHE) in non-collinear antiferromagnets (AFMs) represents a striking Weyl phase, which is associated with Bloch-band topological features. In this work, we report robust AHE and Lifshitz transition in high-quality Weyl semimetal Mn3Ge thin film, comprising stacked Kagome lattice and chiral antiferromagnetism. We successfully achieved giant AHE in our Mn3Ge film, with a strong Berry curvature enhanced by the Weyl phase. The enormous coercive field HC in our AHE curve at 5 K reached an unprecedented 5.3 T among hexagonal Mn3X systems. Our results provide direct experimental evidence of an electronic topological transition in the chiral AFMs. The temperature was demonstrated to play an efficient role in tuning the carrier concentration, which could be quantitatively determined by the two-band model. The electronic band structure crosses the Fermi energy level and leads to the reversal of carrier type around 50 K. The results not only offer new functionality for effectively modulating the Fermi level location in topological Weyl semimetals but also present a promising route of manipulating the carrier concentration in antiferromagnetic spintronic devices.
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http://dx.doi.org/10.1039/d0nr07946dDOI Listing
February 2021

sp. nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).

Int J Syst Evol Microbiol 2021 Feb;71(2)

Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, PR China.

A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6, was isolated from surface seawater collected in Daya Bay (Guangdong, China). Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6 belonged to the genus . The closest phylogenetic relatives of SM6 were MSSRF60 (97.38 % 16S rRNA gene sequence pairwise similarity), R-40492 (97.27 %), ATCC 35048 (97.21 %) and LC2-047 (97.3 %). Growth of strain SM6 occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %). The predominant fatty acids (>10 %) were summed feature 3 (C7 or/and C6), C and summed feature 8 (C7 or/and C6). The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6. Average nucleotide identity values between SM6 and its reference species were lower than the threshold for species delineation (95-96 %); DNA-DNA hybridization further showed that the strains shared less than 70 % similarity. On the basis of evidence from the present polyphasic study, strain SM6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SM6 (=KCTC 82076=MCCC 1K04327 ).
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http://dx.doi.org/10.1099/ijsem.0.004647DOI Listing
February 2021

Spatiotemporal dynamics of the total and active Vibrio spp. populations throughout the Changjiang estuary in China.

Environ Microbiol 2020 10 23;22(10):4438-4455. Epub 2020 Jul 23.

College of Marine Life Sciences, and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.

Vibrio is ubiquitously distributed in marine environments and is the most extensively characterized group within Gammaproteobacteria. Studies have investigated Vibrio spp. worldwide, but mostly focused on pathogenic vibrios and based on cultivation methods. Here, using a combination of molecular and culturing methods, we investigated the dynamics of the total and active Vibrio spp. throughout the Changjiang estuary in China. The total Vibrio abundance was higher in summer (~6.59 × 10 copies ml) than in winter (~1.85 × 10 copies ml) and increased from freshwater to saltwater (e.g. 8.04 × 10 to 9.39 × 10 copies ml in summer). The ratio of active to total Vibrio (Va/Vt) revealed a high activity of vibrios, with remarkable differences between freshwater and saltwater (p < 0.05). Based on the community compositions of the culturable, total and active Vibrio, Vibrio atlanticus and Vibrio owensii were the dominant and active species in winter and summer, respectively. The distribution of Vibrio was governed by the effects of diverse environmental factors, such as temperature, salinity, pH, dissolved oxygen and SiO. Our study clearly demonstrates the spatiotemporal dynamics of total and active Vibrio spp. and lays a foundation for fully understanding the ecological roles of marine Vibrio.
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http://dx.doi.org/10.1111/1462-2920.15152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7689709PMC
October 2020

Antibacterial Properties of Bilayer Biomimetic Nano-ZnO for Dental Implants.

ACS Biomater Sci Eng 2020 04 12;6(4):1880-1886. Epub 2020 Mar 12.

The National Engineering Research Center for Bioengineering Drugs and the Technologies; Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, China.

Dental implant surgery has a relatively high incidence of peri-implantitis. In this research, ZnO nanorods and ZnO nanospheres were synthesized by a hydrothermal method. ZnO nanorods first covered the surface of Ti or Ti-Zr, and ZnO nanospheres were then modified as the outermost layer. By these means a dual antibacterial effect could be realized by the rapid release of ZnO nanospheres and the sustained release of ZnO nanorods. Subsequent studies implied that this ZnO nanorods-nanospheres hierarchical structure (NRS) could be stably loaded on the surface of roughened Ti and Ti-Zr slices. The modified materials not only showed excellent antibacterial activities against both and but also showed low cellular cytotoxicity. This ZnO NRS structure is thus expected to be used as a general antimicrobial coating on the surface of Ti (Ti-Zr) in dental implant surgery.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01695DOI Listing
April 2020

Effect of immobilized anthraquinone-2-sulfonate on antibiotic resistance genes and microbial community in biofilms of anaerobic reactors.

J Environ Manage 2021 Mar 14;282:111967. Epub 2021 Jan 14.

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.

Quinone compounds could significantly accelerate anaerobic biotransformation of refractory pollutants. However, the effect of quinone compounds application on the propagation of antibiotic resistance genes (ARGs) in the bio-treatment of these pollutants-containing wastewater is not available. In this study, the catalytic performance of anthraquinone-2-sulfonate immobilized on polyurethane foam (AQS-PUF), changes of ARGs, mobile gene elements (MGEs) and microbial community structure attached on AQS-PUF and PUF in the up-flow anaerobic bioreactors were investigated. The results showed that AQS-PUF could significantly accelerate the decolorization of azo dye RR X-3B. Meanwhile, metagenomics analysis showed that the total absolute abundance of ARGs increased in the presence of the immobilized AQS. Among ARGs, the number of the efflux pump-encoding ARGs in the biofilm of AQS-PUF accounted for 35.7% of the total ARGs, which was slightly higher than that of PUF (32.1%) due to the presence of the immobilized AQS. The relative abundances of ARGs conferring resistance to MLS (macrolide, lincosamide and streptogramin), tetracycline and sulfonamide, which were deeply concerned, reduced 10%, 21.7% and 7.3% in the presence of the immobilized AQS, respectively. Moreover, the immobilized AQS resulted in the decreased relative abundance of plasmids, transposons and class I integrons. Among the detected 31 ARG subtypes located in MGEs, the relative abundances of only lnuF, msrE and mphD in the biofilm of AQS-PUF were over 2-fold higher compared with those in the biofilm of PUF. However, the three ARGs and their host Gammaproteobacteria was not dominant in microbial community. The relative abundances of more ARGs including MLS (lnuB and EreA), tetracycline (tetH) resistance genes located in MGEs decreased, which was attributed to the decreased relative abundance of their hosts. These studies showed that the addition of the immobilized AQS (around 0.25 mM) had a beneficial effect on reducing the spread of ARGs during dyeing wastewater bio-treatment.
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http://dx.doi.org/10.1016/j.jenvman.2021.111967DOI Listing
March 2021

Efficacy of infliximab treatment on the mucosal healing of different intestinal segments in patients with ileocolonic Crohn's disease.

Therap Adv Gastroenterol 2020 21;13:1756284820976923. Epub 2020 Dec 21.

Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, 200072, China.

Background: Mucosal healing (MH) is the key aim of the treat-to-target strategy for patients with Crohn's disease (CD). The efficacy of infliximab (IFX) on MH in different ileocolonic segments is unclear. The aim of this study was to investigate endoscopic MH in different ileocolonic segments in patients with CD who received IFX treatment.

Methods: A retrospective, single-center study was performed in patients with active ileocolonic CD between January 2012 and December 2018. All patients underwent IFX treatment for at least 30 weeks. The MH of five ileocolonic segments was assessed by the Simple Endoscopic Score for CD (SES-CD) at baseline, 14/22 weeks and 30/38 weeks. The SES-CD values were analyzed by a mixed-effects model after the correction for confounding factors.

Results: A total of 101 eligible patients were included. The baseline endoscopic severity was similar across segments. At 30/38 weeks, the greatest changes in the SES-CD ulcer size and ulcerated surface subscores were -94.29% and -94.32% both in the transverse colon ( < 0.0001), and the smallest changes were -67.88% and -69.67% both in the terminal ileum ( < 0.0001) compared with baseline. Stenosis mainly presented in the right colon (12/29, 41.38%). The change in the SES-CD stenosis subscore was -6.25% in the right colon at 30/38 weeks compared with -71.88% at 14/22 weeks ( = 0.0030). At 30/38 weeks, the transverse colon achieved the highest rate of complete MH (CMH) at 81.2%, and the lowest CMH rate occurred in the terminal ileum at 45.6%. Moreover, the degree of improvement in the rectum was negatively correlated with disease progression ( = 0.011).

Conclusions: Ileocolonic segments in CD presented different degrees of endoscopic MH during IFX treatment. The transverse colon showed the highest CMH rate, whereas the right colon with stenosis showed the poorest improvement. The differing propensities of ileocolonic segments may provide an individualized IFX treatment strategy.
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http://dx.doi.org/10.1177/1756284820976923DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758567PMC
December 2020