Publications by authors named "Xiaolan Cai"

20 Publications

  • Page 1 of 1

Anti-inflammatory Effects of Sweroside on LPS-Induced ALI in Mice Via Activating SIRT1.

Inflammation 2021 Apr 29. Epub 2021 Apr 29.

Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.

Sweroside, as one of the main components of Swertia L. in Gentianaceae, has the effect of clearing heat and detoxifying. In previous studies, sweroside has been reported to have anti-inflammatory effect on LPS-induced inflammation by alleviating NF-κB signaling pathway. In this paper, we investigate the anti-inflammatory effects of sweroside by establishing LPS-induced acute lung injury (ALI) model in mice. Experimental results showed that sweroside could reduce the wet-to-dry ratio of the lung and inhibit MPO activity. In addition, it turned out that sweroside reduced pathological changes in lung tissue and the numbers of inflammatory cells. Moreover, sweroside significantly reduced the levels of inflammatory cytokines and down-regulated the NF-κB signaling pathway. And the results demonstrated that sweroside could increase the expression of SIRT1, and the protective effects of sweroside on LPS-induced ALI were reversed by SIRT1 inhibitor EX-527. In conclusion, sweroside can protect LPS-induced ALI mice through inhibiting inflammation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10753-021-01473-4DOI Listing
April 2021

Carbonaceous Hosts for Sulfur Cathode in Alkali-Metal/S (Alkali Metal = Lithium, Sodium, Potassium) Batteries.

Small 2021 Apr 28:e2006504. Epub 2021 Apr 28.

Institute for Superconducting and Electronic Materials, Australian Institute of Innovative Materials, Innovation Campus, University of Wollongong, Wollongong, NSW, 2500, Australia.

Alkali-metal/sulfur batteries hold great promise for offering relatively high energy density compared to conventional lithium-ion batteries. By providing viable sulfur composites that can be effectively used, carbonaceous hosts as a key component play critical roles in overcoming the preliminary challenges associated with the insulating sulfur and its relatively soluble polysulfides. Herein, a comprehensive overview and recent progress on carbonaceous hosts for advanced next-generation alkali-metal/sulfur batteries are presented. In order to encapsulate the highly active sulfur mass and fully limit polysulfide dissolution, strategies for tailoring the design and synthesis of carbonaceous hosts are summarized in this work. The sticking points that remain for sulfur cathodes in current alkali-metal/sulfur systems and the future remedies that can be provided by carbonaceous hosts are also indicated, which can lead to long cycling lifetimes and highly reversible capacities under repeated sulfur reduction reactions in alkali-metal/sulfur during cycling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202006504DOI Listing
April 2021

Deployment of a bioabsorbable plate as the rigid buttress for skull base repair after endoscopic pituitary surgery.

Gland Surg 2021 Mar;10(3):1010-1017

Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, China.

Background: Bioresorbable alloplastic implants have become desirable as a rigid buttress for reconstructing skull base defects. This study aimed to describe the use of a biodegradable plate (PolyMax RAPID) in skull base repair of endoscopic endonasal pituitary surgery and to investigate the clinical outcome and safety of this novel method.

Methods: Between January 2019 and January 2020, 22 patients with pituitary adenomas who underwent endoscopic skull base repair with a Polymax RAPID plate were included. After endonasal transsphenoidal surgery, a trimmed bioresorbable plate was placed in the position between the dura and the bone of the skull base to reconstruct the sellar floor and buttress the pituitary gland and sellar packing. The patient demographics, radiologic imaging, and postoperative outcomes were carefully reviewed. All patients were followed up by a routine nasal endoscopic assessment and radiologic examinations.

Results: The present study comprised 10 (45.5%) males and 12 (54.4%) females with an average age of 51.9 years. There were 7 (31.8%) growth hormone (GH) secreting adenomas, 2 (9.1%) thyroid stimulating hormone (TSH) secreting adenomas, and 13 (59.1%) non-functioning adenomas. Enlarged sellar floor and paranasal sinusitis were seen in 13 (59.1%) and 11 (50.0%) cases shown by preoperative computed tomography (CT) or magnetic resonance imaging (MRI), respectively. There were 6 (27.3%) grade-1 and 16 (72.7%) grade-0 cases by intraoperative cerebrospinal fluid (CSF) leak grading. None of these patients received lumbar drains postoperatively and no postoperative CSF rhinorrhea was detected in our series. The PolyMax RAPID plates which could be clearly identified on postoperative CT or sagittal T1-weighted MRI were shown to provide an ideal rigid buttress for sellar repair.

Conclusions: The Polymax RAPID plate can be an optimal implant to achieve rigid repair of sellar floor defects after endonasal transsphenoidal pituitary surgery.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/gs-20-642DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033064PMC
March 2021

Identification of DNA methylation module in seasonal allergic rhinitis.

Int J Pediatr Otorhinolaryngol 2019 Feb 29;117:163-166. Epub 2018 Nov 29.

Department of Otolaryngology, Qilu Hospital, Shandong Province, PR China.

Objective: In this study, we aimed to characterize the significant DNA methylation module of seasonal allergic rhinitis.

Methods: Methylation profiling E-GEOD-50222 was obtained from ArrayExpress database. Differential co-methylation network (DCN) was constructed based on the methylation data. From the DCN, we characterized multiple differential modules (M-DMs). Significant module was mapped to pathways to identify significant enriched pathways.

Results: At the criteria of absolute Pearson coefficient value > 0.8, the edges were chose to construct DCN. In the DCN, 16 seed genes were identified. Seed genes were used to construct M-DMs. After statistical analysis, one significant module with p < 0.05 were obtained. After pathways enrichment analysis, 17 significant pathways with p < 0.05 were obtained, and most of these pathways were associated with DNA replication.

Conclusion: One multiple differential module was identified in SAR, and seventeen significant pathways mapped by the module were identified as important factors in SAR. These results may provide new insights into the molecular mechanism of DNA methylation in SAR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijporl.2018.11.030DOI Listing
February 2019

Inflammatory infiltration and tissue remodeling in nasal polyps and adjacent mucosa of unaffected sinus.

Int J Clin Exp Pathol 2018 1;11(5):2707-2713. Epub 2018 May 1.

Department of Otolaryngology, Qilu Hospital, Shandong University Jinan 250012, China.

Background: This study was designed to explore the characteristics of inflammatory infiltration and tissue remodeling in the adjacent unaffected sinus mucosa of the polyp tissue (ANP).

Methods: Nasal polyps (NP) and ANP were obtained from 24 CRSwNP patients who received endoscopic sinus surgery. The frequency and distribution of Eosinophils, T lymphocytes (CD4+ T cells and CD8+ T cells), B lymphocytes, native macrophages, regulatory T cells and the total of inflammatory cells were detected by immunohistochemistry and hematoxylin-eosin stain. The thickness of the basal membrane was evaluated.

Results: Multivariate analysis of Variance (MANOVA) and F-tests were conducted for each independent variable between two groups. With test criterion alpha = 0.05, significant differences were observed between NP and ANP groups in terms of CD3 (F-Value = 10.47, -value = 0.0120), CD4 (F-Value = 9.03, -value = 0.0169), CD8 (F-Value = 17.03, -value = 0.0033) and regulatory T cells (F-Value = 60.42, -value <0.0001). Wilks' Lambda test (F-Value = 25.74, -value = 0.1513) was conducted and no significant difference was observed between the NP group and the ANP group. The percentage of regulatory T cells in ANP was significantly higher than that in NP (3.7110±0.2395 vs 14.6300±1.8360).

Conclusion: ANP and NP may be one disease entity. Treg cells have impacts on the morphology of the tissues and might be a key factor in the further development of ANP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958245PMC
May 2018

High-performance silver nanoparticles coupled with monolayer hydrated tungsten oxide nanosheets: The structural effects in photocatalytic oxidation of cyclohexane.

J Colloid Interface Sci 2018 Apr 3;516:172-181. Epub 2018 Feb 3.

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China. Electronic address:

The photocatalytic properties of silver nanoparticles (Ag NPs) coupled with tungsten oxide (WO) nanocrystals are investigated to understand structural effects of the WO nanocrystals in selective oxidation of cyclohexane (CyH). The photocatalytic activity of monolayer hydrated WO nanosheet - Ag nanoparticle composites (WO NSs-Ag NPs) is 1.3 times higher than that of WO nanocube - Ag nanoparticle composites (WO NCs-Ag NPs). The highest cyclohexane conversion of 40.2% with cyclohexanol and cylohexanone (KA oil) selectivity of 97.0% is achieved by the photocatalyst of WO NSs-Ag NPs under solar-light irradiation at room temperature. The WO NSs-Ag NPs shows good photocatalytic stability without evident decline of catalytic activity after ten cycles. The improved photocatalytic activity in the oxidation of cyclohexane by WO NSs-Ag NPs is mainly due to the facilitated generation of high-reactive hydroxyl radical (OH), which is caused by the enhanced light absorption by surface plasmon resonance (SPR) effect of Ag NPs, and the effective charge transfer on the surface of WO monolayer structure. The design and structural analysis of the WO NSs-Ag NPs in this research may provide a novel approach for the further development of high-performance photocatalyst.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2018.01.057DOI Listing
April 2018

E26 Transformation-Specific Transcription Factor ETS2 as an Oncogene Promotes the Progression of Hypopharyngeal Cancer.

Cancer Biother Radiopharm 2017 Nov 7;32(9):327-334. Epub 2017 Nov 7.

1 Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key Laboratory of Otolaryngology, NHFPC (Shandong University) , Jinan, China .

The E26 transformation-specific (ETS) family is one of the largest families of transcription factors. Upon activation by MAPK pathway, ETS participates in cell proliferation, differentiation, migration, apoptosis, and metastasis. However, the mechanism by which ETS is deregulated in cancer is unclear. In this study, the authors investigated the role of ETS factor, ETS2, in hypopharyngeal cancer pathogenesis in hypopharyngeal cancer tissues (N = 20) and corresponding non-neoplastic tissues (N = 20). The results showed that expression of ETS2 was increased in cancer tissues as compared with the expression in corresponding non-neoplastic tissues. Analysis of clinicopathological characteristics showed that increased level of ETS2 is associated with III-IV tumor node metastasis stage and lymph node metastasis. In addition, knockdown of ETS2 by siRNA in pharyngeal cancer cell line, FaDu, significantly decreased cell's vitality and colony-forming ability by inducing caspase-3-dependent apoptosis and cell cycle arrest. Furthermore, inhibition of ETS2 could abrogate the migration, invasion, and transforming growth factor-β-induced epithelial mesenchymal transition through the upregulation of E-cadherin, zona occludens protein-1, together with downregulation of vimentin and α-sooth muscle actin. These functions of ETS2 could be associated with the activation of MAPK/p38/ERK/JNK signals. Taken together, the authors opined that ETS2 functions as an oncogene and plays a key role in the progression of hypopharyngeal cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/cbr.2017.2296DOI Listing
November 2017

Role of flexible fiberoptic laryngoscopy in predicting difficult intubation.

Minerva Anestesiol 2018 03 4;84(3):337-345. Epub 2017 Oct 4.

Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Jinan, China -

Background: The ability to precisely predict which intubations will be difficult during administration of anesthesia is an important part of preoperative preparation. This study's goal is to accurately identify patients who will be difficult to intubate using the number of tracheal rings observed preoperatively by fiberoptic laryngoscopy.

Methods: We enrolled 994 adult patients in our study who required general anesthesia and orotracheal intubation for their elective surgeries. All patients received a Mallampati Test, a Wilson Risk-Sum Score, and fiberoptic laryngoscopy before operation. Each patient's age, Body Mass Index (BMI), and neck circumference was recorded preoperatively. Logistic regression analysis was applied to evaluate the association between the recorded risk factors and a potentially difficult intubation. The three preoperative assessments were compared using three parameters: positive predictive value, sensitivity, and specificity.

Results: The risk factors which were determined to be predictive for difficult intubation were: BMI, neck circumference, Mallampati Test, Wilson Risk-Sum Score, and fiberoptic laryngoscopy (P<0.05). Fiberoptic laryngoscopy as a predictive factor in the preoperative setting had a higher sensitivity, specificity, and positive predictive value than did the Mallampati Test or the Wilson Risk-Sum Score (P<0.05).

Conclusions: Fiberoptic laryngoscopy is a more accurate and convenient preoperative method to predict difficult intubation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.23736/S0375-9393.17.12144-9DOI Listing
March 2018

The effect and mechanism of action of metformin on in vitro FaDu cell proliferation.

J Int Med Res 2016 Oct 29;44(5):1049-1054. Epub 2016 Sep 29.

1 Department of Otorhinolaryngology, Qilu Hospital of Shandong University, China.

Objective To investigate the effect and mechanism of action of metformin on proliferation of a human hypopharyngeal carcinoma cell line (FaDu). Methods FaDu cells were treated with metformin (25-125 mmol/l). Cell proliferation was evaluated via CCK-8 assay. Real-time quantitative reverse transcription-polymerase chain reaction was used to evaluate microRNA (miR)-21-5p and PDCD4 (programmed cell death 4) expression. PDCD4 protein was quantified by Western blot. Results Metformin significantly inhibited FaDu cell proliferation in a dose- (25-100 mmol/l) and time-dependent manner (12 h-36 h), significantly downregulated miR-21-5p, and upregulated PDCD4 mRNA and protein expression. Conclusions Metformin significantly inhibited FaDu cell proliferation , possibly via downregulation of miR-21-5p and upregulation of PDCD4.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0300060516642645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5536548PMC
October 2016

Prognostic significance of claudin-1 and cyclin B1 protein expression in patients with hypopharyngeal squamous cell carcinoma.

Oncol Lett 2016 May 16;11(5):2995-3002. Epub 2016 Mar 16.

Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

Claudin-l and cyclin B1 are abnormally expressed in certain malignancies, but their expression in hypopharyngeal squamous cell carcinoma (HSCC) has not been reported thus far. Studying the expression levels of claudin-1 and cylin B1 in HSCC tissues and their association with clinical stage, pathological grade and prognosis in patients with HSCC may provide a theoretical basis and guide future research on HSCC targeted therapy. The protein expression levels of the above two biomarkers was immunohistochemically detected in 97 HSCC cases and 90 matched adjacent tissue samples. The correlation between the expression levels of claudin-1 and cylin B1 and the patients' clinical parameters was analyzed via Pearson's χ test, while survival analysis was performed using a log-rank test. The results of the current study revealed that claudin-1 and cyclin B1 were highly expressed in HSCC tissues, and the expression of claudin-1 was associated with tumor differentiation degree and lymph node metastasis, while cyclin B1 expression was associated with tumor differentiation degree. Furthermore, Kaplan-Meier analysis revealed that claudin-1 expression correlated with survival (P=0.003), and the expression levels of claudin-1 and cyclin B1 were observed to be positively correlated, in patients with HSCC. Cyclin B1 and claudin-1 exhibited an elevated expression in HSCC specimens, thus suggesting their use as tumor markers. Therefore, the joint detection of claudin-1 and cyclin B1 may aid to guide cancer therapy and to determine prognosis in HSCC. Furthermore, claudin-1 may be used as an HSCC-monitoring index, and may serve as a therapeutic target.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2016.4333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840523PMC
May 2016

[Application of endotracheal intubation in the rescue of children with severe trachea and bronchial foreign body].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015 Jun;29(11):988-90

Objective: To discuss the application of endotracheal intubation in the rescue of children with severe trachea and bronchial foreign body. By observing the patient's clinical pathological characteristics, the diagnosis and treatment experience were summarized.

Method: A retrospective analysis of 10 children with severe tracheal and bronchial foreign bodies were performed. They were given emergency intubation and cardiopulmonary resuscitation to relieve respiratory and circulatory failure after reaching hospital. After the restoration of spontaneous respiration, oxygen saturation increased by more than 90 percent and vital signs stable, the foreigh body was removed with bronchoscopy. The role of endotracheal intubation before and after the treatment of tracheal and bronchial foreign bodies in children was observed.

Result: Ten cases of children were rescued successfully. No one died and the complications such as cerebral palsy, pneumothorax and pneumomediastinum didn't happened.

Conclusion: For the children with severe tracheal and bronchial foreign body, endotracheal intubation can establish effective ventilation in a shorter period of time. The earlier endotracheal intubation, the shorter time required and the higher success rate. Endotracheal intubation can increase the survival rate of children with cardiopulmonary resuscitation, and can reduce disability.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2015

[Expression of YKL-40 and TLR4 in patients with chronic rhinosinusitis].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2015 Apr;50(4):300-5

Email:

Objective: To explore the expression of YKL-40, TLR4 and NF-κB in chronic rhinosinusitis (CRS) with or without nasal polyps (CRSwNP and CRSsNP), and to investigate their expressional correlation and the potential role in pathogenesis of CRS.

Methods: The specimens were obtained from sinus mucosa and inferior turbinate mucosa of the patients with informed consent. The different expression of YKL-40, TLR4 and NF-κB among each group was detected by real time RT-PCR and immunohistochemistry (S-P method). SPSS 17.0 software was used to analyze the data.

Results: mRNA level: The relative expression of YKL-40 in CRSwNP group (0.91±0.17) was higher than those in the control group (0.49±0.09), the difference was significant (t=2.12, P<0.05). The relative expression of TLR4 in CRSsNP group (0.88±0.19) and CRSwNP group (0.67±0.13) were lower than those in control group (1.48±0.14), the differences were significant (t value was -4.11, -2.48, all P<0.05). The relative expression of NF-κB in CRSsNP group (0.69±0.13) and CRSwNP group (0.72±0.14) were lower than those in control group (1.20±0.15), the differences were significant (t value was 2.33, 2.27, all P<0.05). Protein level: The expression of YKL-40 in CRSwNP group was stronger than that in CRSsNP group and control group (U value was 72.5 and 73, all P<0.01). The expression of TLR4 in CRSwNP group and CRSsNP group was weaker than that in control group (U value was 62 and 38, all P<0.01). There was a negative correlation between YKL-40 and TLR4 (rmRNA=-0.741, P<0.01; rprotein=-0.46, P<0.05) in CRSwNP group.

Conclusions: The expression of YKL-40 in pantients with CRSwNP is higher than those in healthy control and CRSsNP patients. There was a negative correlation between YKL-40 and TLR4. Both of them may be involved in the pathogenesis of CRSwNP.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2015

[Endoscopic surgery for maxillary sinus inverted papilloma].

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014 Sep;49(9):721-5

Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Ji'nan 250012, China.

Objective: To evaluate the effect of endoscopic surgery for maxillary sinus inverted papilloma.

Methods: From 2000 to 2011, 50 patients with maxillary sinus inverted papilloma were treated with intranasal endoscopic surgery or combined approach. Among them, 39 cases were primary and 11 cases were recurrent, which included 17 patients with Krouse stage IIand 33 cases with Krouse stage III. Different surgical approaches were selected according to the range and positions of the lesions. One case with severe atypical hyperplasia received postoperative radiotherapy.

Results: All cases were followed up for 3-14 years, 7 cases recurred. All occurrence within 2 years after operation. Malignant change and death was found in 1 case. After operation, epiphora occurred in 2 cases, maxillary sinus labiogingival groove fistula occurred in 2 cases, facial numbness occurred in 4 cases and incision hemorrhage occurred in 1 case. All the complications were cured after appropriate treatment.

Conclusions: Naso-endoscopic surgery or combined approach is a safe, effective and microinvasive treatment for patients with maxillary sinus inverted papilloma. Accurate selection of suitable approach according to the range and positions of the lesions and correct management of the base of the tumor are critical to the success of the operation.
View Article and Find Full Text PDF

Download full-text PDF

Source
September 2014

Ultrathin CdSe nanosheets: synthesis and application in simultaneous determination of catechol and hydroquinone.

Anal Chim Acta 2012 Nov 1;752:101-5. Epub 2012 Oct 1.

School of Biochemical and Pharmaceutical Sciences, Capital Medical University, Beijing, China.

Ultrathin crystalline CdSe nanosheets have been synthesized through a facile solution processes. Their application to simultaneous electrochemical determination of catechol and hydroquinone is demonstrated. The few-layer single crystalline CdSe modified electrode exhibits strong electrocatalytic activity toward the oxidation of a mixture of catechol and hydroquinone. The excellent analytical performance makes the ultrathin CdSe nanomaterials promising for the development of electrochemical sensors for potential applications in medicine, biotechnology and environmental chemistry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2012.09.034DOI Listing
November 2012

CIAPIN1 nuclear accumulation predicts poor clinical outcome in epithelial ovarian cancer.

World J Surg Oncol 2012 Jun 19;10:112. Epub 2012 Jun 19.

Department of Gynecology and Obstetrics, Xijing Hospital, the Fourth Military Medical University, Number 15, Changle Western Road, Xi'an, 370032, China.

Background: Epithelial ovarian cancer (EOC) is an aggressive disease with poor prognosis. The expression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) correlates with the malignant progression of several cancers. However, the relationship between the subcellular localization of CIAPIN1 and clinical characteristics in EOC remains unclear.

Methods: Immunohistochemistry was performed to detect CIAPIN1 expression in 108 EOC tissues. CIAPIN1 expressions in eight fresh EOC tissues were detected by Western blotting. The relationship between CIAPIN1 subcellular expression and patients' clinicopathological features, including prognosis, was evaluated. Immunohistochemistry and immunofluorescence were employed to assess the CIAPIN1 subcellular localization in the EOC cell lines A2780 and HO8910. In addition, all patients were followed up to assess the prognostic value of CIAPIN1 in patients with EOC.

Results: CIAPIN1 is highly expressed in EOC, but is present at low levels in paired non-cancerous ovarian epithelial tissues. The results of Western blotting were in accordance with the immunohistochemical results. Poor differentiation of the tumors and EOC cell lines correlated with higher levels of CIAPIN1 nuclear expression. CIAPIN1 nuclear expression significantly correlated with the Federation International of Gynecology and Obstetrics (FIGO) stage and histological differentiation (P = 0.034 and P < 0.0001, respectively). Moreover, nuclear localization of CIAPIN1 was selected as an unfavorable prognostic factor by both univariate and multivariate analyses ( P < 0.001). However, no significant correlations were observed between cytoplasmic localization of CIAPIN1 and clinicopathological parameters.

Conclusions: CIAPIN1 might play a crucial role in the differentiation of EOC cells. Elevated expression of nuclear CIAPIN1 negatively correlated with the survival of EOC patients, suggesting that nuclear CIAPIN1 might serve as a prognostic biomarker for EOC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/1477-7819-10-112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3502349PMC
June 2012

RNA interference of caveolin-1 via lentiviral vector inhibits growth of hypopharyngeal squamous cell carcinoma FaDu cells In Vitro and In Vivo.

Asian Pac J Cancer Prev 2011 ;12(2):397-401

Department of Otolaryngology, Qilu Hospital, Shandong University, Jinan, PR China.

Objective: To investigate the effects of caveolin-1 (CAV1) on the growth of hypopharyngeal squamous cell carcinoma (HSCC) FaDu cells in vitro and in vivo.

Methods: A CAV1-RNAi-lentivirus construct was transfected into FaDu cells and expression of caveolin-1 was tested by RT-PCR and western blotting analysis. Cell apoptosis was analyzed by transferase-medisated dUTP nick-end labeling (TUNEL) assay. Tumor inhibition effects were investigated by injecting rCAV1-RNAi-lentivirus construct into tumors created with FaDu cells in the HSCC mouse model, with the empty-vector lentivirus as a control. CAV1 expression in xenografts was tested by RT-PCR and immunohistochemistry.

Results: RT-PCR and western blot analysis demonstrated successful construction of the CAV1-RNAi-lentivirus construct producing small hairpin RNA. The average weights and volumes of tumor in mice treated with CAV1-RNAi-lentivirus were lower than in mice with control treatment (P < 0.05). RT-PCR revealed weak positive expression of CAV1 in CAV1-construct-treated xenografts and immunohistochemistry confirmed lower CAV1 expression than in controls.(P < 0.05). In addition, downregulation of CAV1 increased cell apoptosis in vitro.

Conclusion: The growth of HSCCs could be inhibited by recombinant CAV1-RNAi-lentivirus in vitro and in vivo.
View Article and Find Full Text PDF

Download full-text PDF

Source
August 2011

Hierarchical CuO nanochains: synthesis and their electrocatalytic determination of nitrite.

Anal Chim Acta 2011 Apr 23;691(1-2):43-7. Epub 2011 Feb 23.

School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing, China.

Microspheres with multilevel structure are widely used as removable catalysts, adsorbents, drug carriers and imaging contrast agents due to their hierarchical porosity. In this paper a facile wet chemical method for the synthesis of hierarchically nanostructured CuO chains assembled with small nanorods is reported. The as prepared nanomaterial is characterized using X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HRTEM). The autocatalytic response to nitrite ions is also carried out, which exhibits a wide linear range (0.004-3.7 mM), sound sensitivity (177.9 μA mg(-1) dL(-1) cm(-2)), highly reproducible response (R.S.D. of 2.0%), and an excellent long-term stability. The good analytical performance, low cost and straightforward preparation method make this novel electrode material promising for the development of effective nitrite sensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aca.2011.02.037DOI Listing
April 2011

Porous cuprite films: facile solution deposition and their application for nitrite sensing.

Analyst 2010 Aug 8;135(8):2106-10. Epub 2010 Jun 8.

Beijing University of Aeronautics and Astronautics, Beijing, 100083, China.

Nanostructured porous cuprite (NPC) films were first prepared by a facile wet chemical process. The resulted cuprite film was then characterized by Raman spectrum, scanning electron microscopy (SEM), transmission electron microscopy (TEM), as well as static contact angle experiment. Electrochemical catalytic performance of the NPC film was also tested. The low detection limit was estimated to be 6.3 microM (S/N = 3), and the time required to reach 95% of the steady-state current was shorter than 10 s after the addition of nitrite, which demonstrated that the as deposited nanostructured cuprite film has both high catalytic sensitivity and fast current response in nitrite sensing. The self-supporting porous nanostructure and their good contact with the conducting substrate resulted in large specific area, structure stability and efficient transportation. The good electrocatalytic performance shows that the nanoparticle-assembled thin films with nanoporous structure and nanocrystallites have potential applications as electrocatalysis platforms in biosensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c000086hDOI Listing
August 2010

[Preliminary design for a VI system combining the voice acoustic analyzing and glottal image analyzing].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2008 Apr;25(2):291-4

Department of Biomedical Engineering, Medical School, Shandong University, Jinan 250012, China.

This work is directed at developing a virtual instrument system as an accessorial diagnostic instrument for laryngeal diseases. Programmed with LabWindows/CVI, the system combines the voice acoustic analyzing function with the glottal image measuring function. The voice acoustic analyzing system can sample, store and replay vocal signals; can extract and analyze parameters, including fundamental frequency (F0), frequency perturbation quotient (FPQ), amplitude perturbation quotient(APQ), harmonics-to-noise ratio (HNR), jitter frequency (JF), Shimmer; and can do 3D sound graph analysis. The glottal image analyzing system can sample and store the image observed by the laryngostroboscope; can display any phase in one cycle of the vibration of the vocal cords or a slow and continuous movement of vibrating vocal cords; can snap and save the diagnostic frame of image; and can extract the parameters of the image such as the length and area of the glottis, the length and area of the vocal cords and the diseased part.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2008

[Diagnosis and treatment of nasal sinus osteoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 Mar;21(5):217-8

Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan, 250012, China.

Objective: To discuss the clinical characteristic and surgical treatment of nasal sinus osteoma.

Method: Fifty-one cases with nasal sinus osteoma were diagnosed by nasal sinus X ray or CT scan. All of these osteoma patients were cured by surgical resection. Supraorbital Lynch approach were applied in forty-two cases, lateral rhinotomy Moure approach in 4 cases, Caldwell-Luc approach in 3 cases and Dandy approach that combined with cranium and facial route in 2 cases.

Result: Operative incisions in all cases healed in 1 period, no complications such as brain spinal fistula occurred. All cases were followed up for 6 months to 5 years, there were no recurrence of osteomas and occurrence of mucus cysts.

Conclusion: Osteomas in nasal sinus grow slowly. X ray or CT scan is helpful to confirm the diagnosis and choose the surgical approach reasonably.
View Article and Find Full Text PDF

Download full-text PDF

Source
March 2007