Publications by authors named "Xiaojun Zhang"

587 Publications

Comparison between F-DCFPyL PET and MRI for the detection of transition zone prostate cancer.

Prostate 2021 Sep 13. Epub 2021 Sep 13.

Department of Nuclear Medicine, Chinese PLA General Hospital, Beijing, China.

Background: We aimed to compare the diagnostic performance of F-DCFPyL positron emission tomography (PET) and multiparameter magnetic resonance imaging (mp-MRI) in detecting transition zone (TZ) prostate cancer (PCa).

Methods: This retrospective study included 20 patients who underwent F-DCFPyL PET/MRI and 32 patients who underwent F-DCFPyL PET/CT and MRI from January 2019 to June 2020. All patients had TZ lesions and underwent prostate biopsies. One senior (reader 1) and one junior (reader 2) nuclear medicine physician evaluated each TZ lesion independently, according to the molecular imaging prostate-specific membrane antigen scoring system and the Prostate Imaging Reporting and Data System version 2.1 (PI-RADS v2.1). The histologic diagnosis of prostate biopsy was used as the reference standard. The diagnostic performance of the two methods was compared in terms of inter-reader agreement and area under the receiver operating characteristic (AUC-ROC) curve.

Results: Of the 52 patients, 43 had TZ PCa. For inter-reader agreement, the kappa value was 0.883 for F-DCFPyL PET and 0.393 for mp-MRI. For PET, both readers had the same diagnostic sensitivity, specificity, and accuracy of 93.0%, 77.8%, and 90.4%, respectively. For mp-MRI, the diagnostic sensitivity, specificity, and accuracy was 67.4%, 33.3%, and 61.5% for reader 1, and 51.2%, 44.4%, and 51.9% for reader 2, respectively. PET outperformed mp-MRI for both readers with an AUC of 0.872 for PET versus 0.584 for mp-MRI, p = .0209 for reader 1, and an AUC of 0.860 for PET versus 0.505 for mp-MRI, p = .0213 for reader 2. Among the 43 patients with TZ PCa, F-DCFPyL PET detected a distant bone metastasis missed by the CT in one case and two small lymph node metastases missed by the CT and MRI in another case.

Conclusions: These results suggest that F-DCFPyL PET, which was almost independent of the experience of the readers, was more objective in the evaluation of TZ lesions, and had higher diagnostic value than mp-MRI.
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http://dx.doi.org/10.1002/pros.24230DOI Listing
September 2021

Towards sustainable agriculture: rhizosphere microbiome engineering.

Appl Microbiol Biotechnol 2021 Sep 11. Epub 2021 Sep 11.

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China.

Soil microbiomes are extremely complex, with dense networks of interconnected microbial species underpinning vital functions for the ecosystem. In advanced agricultural research, rhizosphere microbiome engineering is gaining much attention, as the microbial community has been acknowledged to be a crucial partner of associated plants for their health fitness and yield. However, single or combined effects of a wide range of soil biotic and abiotic factors impact the success of engineered microbiomes, as these microbial communities exhibit uneven structural and functional networks in diverse soil conditions. Therefore, once a deep understanding of major influential factors and corresponding microbial responses is developed, the microbiome can be more effectively manipulated and optimized for cropping benefits. In this mini-review, we propose the concept of a microbiome-mediated smart agriculture system (MiMSAS). We summarize some of the advanced strategies for engineering the rhizosphere microbiome to withstand the stresses imposed by dominant abiotic and biotic factors. This work will help the scientific community gain more clarity about engineered microbiome technologies for increasing crop productivity and environmental sustainability.Key points• Individual or combined effects of soil biotic and abiotic variables hamper the implementation of engineered microbiome technologies in the field.• As a traditional approach, reduced-tillage practices coinciding with biofertilization can promote a relatively stable functional microbiome.• Increasing the complexity and efficiency of the synthetic microbiome is one way to improve its field-application success rate.• Plant genome editing/engineering is a promising approach for recruiting desired microbiomes for agricultural benefit.
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http://dx.doi.org/10.1007/s00253-021-11555-wDOI Listing
September 2021

Clemastine Ameliorates Perioperative Neurocognitive Disorder in Aged Mice Caused by Anesthesia and Surgery.

Front Pharmacol 2021 23;12:738590. Epub 2021 Aug 23.

Department of Anesthesiology, Sun Yat-Sen Memorial Hospital, Guangzhou, China.

Perioperative neurocognitive disorder (PND) leads to progressive deterioration of cognitive function, especially in aged patients. Demyelination is closely associated with cognitive dysfunction. However, the relationship between PND and demyelination remains unclear. Here we showed that demyelination was related to the pathogenesis of PND. Clemastine, an antihistamine with potency in remyelination, was predicted to have a potential therapeutic effect on PND by next-generation sequencing and bioinformatics in our previous study. In the present study, it was given at 10 mg/kg per day for 2 weeks to evaluate the effects on PND in aged mice. We found that clemastine ameliorated PND and reduced the expression levels of inflammatory factors such as tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β). Further investigation suggested clemastine increased the expression of oligodendrocyte transcription factor 2 (OLIG2) and myelin basic protein (MBP) to enhance remyelination by inhibiting the overactivation of the WNT/β-catenin pathway. At the same time, the expression of post-synaptic density protein 95 (PSD95, or DLG4), brain-derived neurotrophic factor (BDNF), synaptosomal-associated protein 25 (SNAP25) and neuronal nuclei (NEUN) were also improved. Our results suggested that clemastine might be a therapy for PND caused by anesthetic and surgical factors in aged patients.
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http://dx.doi.org/10.3389/fphar.2021.738590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419266PMC
August 2021

Ga-labeled ODAP-Urea-based PSMA agents in prostate cancer: first-in-human imaging of an optimized agent.

Eur J Nucl Med Mol Imaging 2021 Aug 28. Epub 2021 Aug 28.

Department of Nuclear Medicine, Peking University First Hospital, Beijing, 100034, China.

Purpose: Prostate-specific membrane antigen (PSMA) is a promising target for prostate cancer imaging and therapy. The most commonly used scaffold incorporates a glutamate-urea (Glu-Urea) function. We recently developed oxalyldiaminopropionic acid-urea (ODAP-Urea) PSMA ligands in an attempt to improve upon the pharmacokinetic properties of existing agents. Here, we report the synthesis of an optimized Ga-labeled ODAP-Urea-based ligand, [Ga]Ga-P137, and first-in-human results.

Methods: Twelve ODAP-Urea-based ligands were synthesized and radiolabeled with Ga in high radiochemical yield and purity. Their PSMA inhibitory capacities were determined using the NAALADase assay. Radioligands were evaluated in mice-bearing 22Rv1 prostate tumors by microPET. Lead compound [Ga]Ga-P137 was evaluated for stability, cell uptake, and biodistribution. PET imaging of [Ga]Ga-P137 was performed in three patients head-to-head compared to [Ga]Ga-PSMA-617.

Results: Ligands were synthesized in 11.1-44.4% yield and > 95% purity. They have high affinity to PSMA (K of 0.13 to 5.47 nM). [Ga]Ga-P137 was stable and hydrophilic. [Ga]Ga-P137 showed higher uptake than [Ga]Ga-PSMA-617 in tumor-bearing mice at 6.43 ± 0.98%IA/g vs 3.41 ± 1.31%IA/g at 60-min post-injection. In human studies, the normal organ biodistribution of [Ga]Ga-P137 was grossly equivalent to that of [Ga]Ga-PSMA-617 except for within the urinary tract, in which [Ga]Ga-P137 demonstrated lower uptake.

Conclusion: The optimized ODAP-Urea-based ligand [Ga]Ga-P137 can image PSMA in xenograft models and humans, with lower bladder accumulation to the Glu-Urea-based agent, [Ga]Ga-PSMA-617, in a preliminary, first-in-human study.

Trial Registration: ClinicalTrials.gov Identifier: NCT04560725, Registered 23 September 2020. https://clinicaltrials.gov/ct2/show/NCT04560725.
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http://dx.doi.org/10.1007/s00259-021-05486-xDOI Listing
August 2021

The Chinese Government's Response to the Pandemic: Measures, Dynamic Changes, and Main Patterns.

Healthcare (Basel) 2021 Aug 8;9(8). Epub 2021 Aug 8.

School of Economics and Management, Fuzhou University, Fuzhou 350108, China.

(1) Background: The governance measures that governments deploy vary substantially across countries and even within countries; there is, however, limited knowledge of the responses of local governments or from different areas in the same country. (2) Methods: By using grounded theory and an automatic text processing method, this study analyses the pandemic governance measures, the pandemic governance pattern, and possible factors across 28 provinces in mainland China based on the text of 28 official provincial government Sina microblogs dating from 20 January to 1 July 2020. (3) Results and discussion: The provincial pandemic governance patterns in China are divided into a pathogen-control pattern, a diagnosis and treatment consolidation pattern, a balanced promotion pattern, a quick-adjustment response pattern, and a recovery-oriented pattern. The pandemic severity, economic development, public health service, and population structure may all have an impact on pandemic governance measures. (4) Conclusions: The conclusions of this study may help us to reconstruct governance systems related to global public health emergencies from the perspective of normalisation, as well as providing important clarification for management and a reference for countries seeking to curb the global spread of a pandemic.
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http://dx.doi.org/10.3390/healthcare9081020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393519PMC
August 2021

Clustering genomic organization of sea cucumber miRNAs impacts their evolution and expression.

Genomics 2021 Aug 8;113(6):3544-3555. Epub 2021 Aug 8.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China. Electronic address:

Echinoderms are marine deuterostomes with fascinating adaptation features such as aestivation and organ regeneration. However, post-transcriptional gene regulation by microRNAs (miRNAs) underlying these features are largely unexplored. Here, using homology-based and de novo approaches supported by expression data, we provided a comprehensive annotation of miRNA genes in the sea cucumber Apostichopus japonicus. By linkage and phylogenic analyses, we characterized miRNA genomic organization, evolutionary history and expression regulation. The results showed that sea cucumbers evolved a large number of new miRNAs, which tended to form polycistronic clusters via tandem duplication that had been especially active in the echinoderms. Most new miRNAs were weakly expressed, but miRNA clustering increased the expression level of clustered new miRNAs. The most abundantly expressed new miRNAs were organized in a single tandem cluster (cluster n2), which was activated during aestivation and intestine regeneration. Overall, our analyses suggest that clustering of miRNAs is important for their evolutionary origin, expression control, and functional cooperation.
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http://dx.doi.org/10.1016/j.ygeno.2021.08.003DOI Listing
August 2021

DLSTM-Based Successive Cancellation Flipping Decoder for Short Polar Codes.

Entropy (Basel) 2021 Jul 6;23(7). Epub 2021 Jul 6.

School of Computer Science and Engineering, Shandong University of Science and Technology, Qingdao 266510, China.

Polar code has been adopted as the control channel coding scheme for the fifth generation (5G), and the performance of short polar codes is receiving intensive attention. The successive cancellation flipping (SC flipping) algorithm suffers a significant performance loss in short block lengths. To address this issue, we propose a double long short-term memory (DLSTM) neural network to locate the first error bit. To enhance the prediction accuracy of the DLSTM network, all frozen bits are clipped in the output layer. Then, Gaussian approximation is applied to measure the channel reliability and rank the flipping set to choose the least reliable position for multi-bit flipping. To be robust under different codewords, padding and masking strategies aid the network architecture to be compatible with multiple block lengths. Numerical results indicate that the error-correction performance of the proposed algorithm is competitive with that of the CA-SCL algorithm. It has better performance than the machine learning-based multi-bit flipping SC (ML-MSCF) decoder and the dynamic SC flipping (DSCF) decoder for short polar codes.
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http://dx.doi.org/10.3390/e23070863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307723PMC
July 2021

Lateral Lymph Node Metastases in T1a Papillary Thyroid Carcinoma: Stratification by Tumor Location and Size.

Front Endocrinol (Lausanne) 2021 15;12:716082. Epub 2021 Jul 15.

Department of Head Neck and Thyroid Surgery, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.

Objective: To analyze the incidence and risk factors for lateral lymph node metastases (LNMs) in T1a papillary thyroid carcinoma (PTC) with a focus on tumor location and size.

Materials And Methods: The incidence of lateral LNM in 345 cases of T1a PTC was retrospectively analyzed. Univariate and multivariate analyses were performed to assess the relationships between lateral LNM and clinicopathological characteristics.

Results: The incidence of skip metastasis to lateral LNM in T1a PTC located in the upper lobe was 12.1% (8/66). Logistic regression analysis indicated tumor size >5 mm (OR = 5.04, 95% CI = 1.79 to 14.18, P = 0.002), upper lobe location (OR = 7.68, 95% CI = 3.05-19.34, P < 0.001) and the number of central neck LNM (<2: OR = 24.79, 95% CI = 8.23-74.60, P < 0.001; ≥2: OR = 4.99, 95% CI = 1.95-12.73, P < 0.001) were independently associated with lateral LNM. Comparing the lateral and central LNM stratification based on tumor location revealed that both the incidences of lateral (33.3%) and central (30.3%) LNM of T1a PTC located in the upper lobe were higher than those of T1a PTC located in the middle and lower lobes. Of T1a PTC located in the upper lobe, the incidence of lateral LNM was 33.3% (22/66), which was higher than that [30.3% (20/66)] of central LNM. This finding is reversed in all T1a PTC cases and T1a PTC cases with tumor located in the middle and lower lobes.

Conclusion: A particularly high likelihood of lateral LNM was observed in T1a PTC patients with tumor located in the upper lobe of the thyroid gland, especially the tumor >5 mm in size, which could be considered a risk factor for lateral LNM in the clinical management of T1a PTC.
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http://dx.doi.org/10.3389/fendo.2021.716082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320373PMC
July 2021

RNA-seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat.

Plant Genome 2021 Jul 26:e20120. Epub 2021 Jul 26.

Institute of Pharmaceutical & Food Engineering, Shanxi Univ. of Chinese Medicine, Jingzhong, 030619, China.

Powdery mildew causes considerable yield losses in common wheat (Triticum aestivum L.) production. Mapping and cloning powdery mildew-resistant quantitative trait loci can benefit stable yield production by facilitating the breeding of resistant varieties. In this study, we used the powdery mildew resistance introgression line 'CH7087' (harboring the resistance gene PmCH7087) and developed a large F population and a corresponding F segregation population containing 2,000 family lines for molecular mapping of PmCH7087. Genetic analysis demonstrated that the resistance phenotype was controlled by a single dominant gene. According to the performance exhibited by the F lines, 50 resistant lines and 50 susceptible lines without phenotype segregation were chosen for pooling and bulked segregant RNA sequencing (BSR-Seq) analysis. A region spanning 42.77 Mb was identified, and genotyping of an additional 183 F lines with extreme phenotypes using 20 kompetitive allele-specific polymerase chain reaction (KASP) markers in the BSR-Seq mapping regions confirmed this region and narrowed it to 9.68 Mb, in which 45 genes were identified and annotated. Five of these transcripts harbored nonsynonymous single nucleotide polymorphisms between the two parents, with the transcripts of TraesCS2B01G302800 being involved in signal transduction. Furthermore, TraesCS2B01G302800.2 was annotated as the closest homologue of serine/threonine-protein kinase PBS1, a typical participant in the plant disease immune response, indicating that TraesCS2B01G302800 was the candidate gene of PmCH7087. Our results may facilitate future research attempting to improve powdery mildew resistance in wheat and to identify candidate genes for further verification and gene cloning.
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http://dx.doi.org/10.1002/tpg2.20120DOI Listing
July 2021

State-based event-triggered consensus strategy for Takagi-Sugeno fuzzy fractional-order multiagent systems with switching topologies.

ISA Trans 2021 Jul 17. Epub 2021 Jul 17.

School of Mathematical Sciences, University of Electronic Science and Technology of China, Chengdu 611731, PR China. Electronic address:

This article addresses the event-triggered consensus problem for Takagi-Sugeno fuzzy fractional-order multiagent systems with switching topologies. First, to effectively avoid the frequent communication among agents, a state-based event-triggered consensus strategy is designed, which uses the local information from neighboring agents at sampling moments. Then, several sufficient conditions, which rely on the fractional derivative number and time delay information, are presented to guarantee the consensus of fractional-order multiagent systems based on Takagi-Sugeno fuzzy model. Moreover, Zeno behaviors are precluded by proving that the interval length of the two consecutive event-triggering moments for each agent is greater than a positive constant. Finally, some numerical examples are presented, which not only demonstrated the rationality of our proposed consensus protocol but also shown that the presented consensus method based on the designed event-triggered control protocol has the advantage for avoiding communication congestion compared to the existing results.
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http://dx.doi.org/10.1016/j.isatra.2021.07.022DOI Listing
July 2021

Development of Sequence-Tagged Site Marker Set for Identification of J, J, and St Sub-genomes of in Wheat Background.

Front Plant Sci 2021 23;12:685216. Epub 2021 Jun 23.

College of Agriculture, Shanxi Agricultural University, Taiyuan, China.

(2 = 6 = 42, JJJJStSt) is one of the important resources for the wheat improvement. So far, a few ()-specific molecular markers have been reported, but the number is far from enough to meet the need of identifying alien fragments in wheat- hybrids. In this study, 5,877,409 contigs were assembled using the genotyping-by-sequencing (GBS) data. We obtained 5,452 non-redundant contigs containing mapped -GBS markers with less than 20% similarity to the wheat genome and developed 2,019 sequence-tagged site (STS) molecular markers. Among the markers designed, 745 -specific markers with amplification products in but not in eight wheat landraces were further selected. The distribution of these markers in different homologous groups of varied from 47 (7/12/28 on 6J/6St/6J) to 183 (54/62/67 on 7J/7St/7J). Furthermore, the effectiveness of these -specific markers was verified using wheat- partial amphidiploids, addition lines, substitution lines, and translocation lines. Markers developed in this study provide a convenient, rapid, reliable, and economical method for identifying chromosomes in wheat. In addition, this set of -specific markers can also be used to estimate genetic and physical locations of chromatin in the introgression lines, thus providing valuable information for follow-up studies such as alien gene mining.
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http://dx.doi.org/10.3389/fpls.2021.685216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261300PMC
June 2021

Identification of the Potential Gene Regulatory Networks and Therapeutics in Aged Mice With Postoperative Neurocognitive Disorder.

Front Neurosci 2021 24;15:689188. Epub 2021 Jun 24.

Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Postoperative neurocognitive disorder (PND) is one of the most common postoperative neurological complications in aged patients, characterized by mental disorder, anxiety, personality changes, and impaired memory. At present, the molecular mechanism of PND remains largely unclear, and the ideal biomarker for clinical diagnosis and prognosis are lacking. Circular RNA (circRNA) and microRNA (miRNA), as unique non-coding RNAs, affecting the regulation of miRNAs on genes and further intervening in the progression of diseases through the sponge action between the two. Besides, it could be served as novel biomarkers in various diseases. In order to detect the differential expression profiles of genes caused by PND, a total of 26 18-month-old male C57BL/6 mice were randomly assigned to control group and PND group. Behavioral tests showed that mice in the PND group had impaired cognitive function compared with the control group. Three mice in each group were randomly selected to harvest the brain for analysis the expressions of circRNAs, miRNAs, and mRNAs in the prefrontal cortex by next-generation sequencing (NGS) technology. Differentially expressed genes, including 1192 circRNAs, 27 miRNAs, and 266 mRNAs were identified, and its accuracy was further confirmed by qRT-PCR. Bioinformatics analysis results suggested that neuroinflammation was the main pathological mechanism of PND. The construction of competitive endogenous RNA (ceRNA) networks and the identification of hub genes provided possible therapeutic targets for PND. Cinnarizine and Clemastine were predicted to have the potential therapeutic effects on PND. This is the first study to explore the differential expression profiles of genes and their regulation mechanisms in PND, our results provided new clues and targets for the treatment of this refractory disease.
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http://dx.doi.org/10.3389/fnins.2021.689188DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264595PMC
June 2021

LDHA-Mediated Glycolytic Metabolism in Nucleus Pulposus Cells Is a Potential Therapeutic Target for Intervertebral Disc Degeneration.

Biomed Res Int 2021 10;2021:9914417. Epub 2021 Jun 10.

Department of Orthopedics, The First Affiliated Hospital, Chongqing Medical University, China.

The intervertebral disc degeneration (IDD) is considered to be an initiator of a series of spinal diseases, among which changes in the nucleus pulposus (NP) are the most significant. NP cells reside in a microenvironment with a lack of blood vessels, hypoxia, and low glucose within the intervertebral disc. Due to the strong activity of HIF-1, glycolysis is the main pathway for energy metabolism in NP cells. Our previous study found that higher SIRT1 expression is beneficial to delay the degeneration of NP cells. In order to find the downstream genes by which SIRT1 acts on NP cells, we used iTRAQ sequencing to detect the differences between degenerated NP cells overexpressing SIRT1 and a control group (human NP cells were derived from surgery) and found that the expression of LDHA changed in the same direction with SIRT1. This suggests that SIRT1 may delay the degeneration of NP cells by regulating glycolysis. We then used a Seahorse XFe24 analyzer to measure the bioenergetic parameters of NP cells and obtained three findings: (a) glycolysis is the main energy metabolism pathway in NP cells, (b) there is a large difference in ATP production between senescent cells and young cells, and (c) SIRT1 can regulate the production of ATP from glycolysis by regulating LDHA. We also found that SIRT1 in NP cells has a positive regulatory effect on c-Myc which is an upstream gene of LDHA. Through observing IDD-related indicators such as apoptosis, proliferation, senescence, and extracellular matrix, we found that SIRT1 can delay degeneration, and interference with c-Myc and LDHA, respectively, weakens the protective effect of SIRT1. Interfering with LDHA alone can also inhibit glycolysis and accelerate degeneration. Overall, we found that the inhibition of glycolysis in Np cells significantly affects their normal physiological functions and determined that LDHA is a potential therapeutic target for the treatment of IDD.
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http://dx.doi.org/10.1155/2021/9914417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213459PMC
June 2021

[Progress on the metabolic rules and detection methods for okadaic acid related toxins in biological samples].

Se Pu 2020 Jun;38(6):621-626

Laboratory of Aquatic Product Processing and Quality Safety, Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316021, China.

In recent years, red tide pollution in China has become increasingly serious, leading to a number of shellfish poisoning cases, thereby posing a threat to human health and safety. Okadaic acid (OA) and its analogs (dinophysistoxins, DTXs) are the most widely distributed diarrhetic shellfish poisons, which result in acute diarrheal toxicity and various types of chronic toxicity. It is imperative to establish a method for determination of OA related toxin residues in biological fluid samples, so that accurate diagnosis of poisoning in patients is possible. The present paper briefly introduced the main physicochemical properties, poisoning incidents, toxicological effects for the toxins, also summarized recent progress on the metabolic rules and detection methods for OA related toxins in biological samples.
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http://dx.doi.org/10.3724/SP.J.1123.2019.10016DOI Listing
June 2020

A pH-sensitive semi-interpenetrating polymer network hydrogels constructed by konjac glucomannan and poly (γ-glutamic acid): Synthesis, characterization and swelling behavior.

Int J Biol Macromol 2021 Aug 10;185:229-239. Epub 2021 Jun 10.

Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address:

A novel pH-sensitive semi-interpenetrating polymer network (semi-IPN) hydrogel was prepared by using konjac glucomannan (KGM) and poly (γ-glutamic acid) (γ-PGA) with sodium trimetaphosphate (STMP) as the crosslinking agent. The structure of the semi-IPN hydrogels was characterized by FTIR spectra, thermogravimetric analysis (TGA), X-ray diffraction (XRD), rheological measurements, and scanning electron microscopy (SEM). The pH-sensitive effects were investigated by calculating the equilibrium swelling ratio (ESR) in buffer solutions (pH 2, 4, 6, and 8, respectively) at 37 °C. These results showed that the content of cross-linker and γ-PGA has a significant influence on the hydrogels' structure and swelling behavior. In vitro drug release behavior of semi-IPN hydrogels was investigated under simulated gastric and intestinal fluids using model drug Nicotinamide (NTM), and various models were applied to describe the drug release behaviors. The obtained results indicated that our synthesized semi-IPN hydrogel had the potential to be used as a suitable biomaterial carrier for functional components or drug delivery in the intestine.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.046DOI Listing
August 2021

Comparison of viscocanalostomy plus suture-assisted near-360-degree trabeculotomy and viscocanalostomy plus rigid probe trabeculotomy in primary congenital glaucoma.

Acta Ophthalmol 2021 Jun 11. Epub 2021 Jun 11.

Department of Ophthalmology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: To compare the efficacy and safety of viscocanalostomy plus near-360-degree suture trabeculotomy (VST) with viscocanalostomy plus rigid probe trabeculotomy (VT) in treating primary congenital glaucoma (PCG) over a one-year follow-up.

Methods: This consecutive retrospective study included patients with PCG confirmed within 3 years of age from March 2017 to October 2019. Efficacy was evaluated by comparing the postoperative intraocular pressure (IOP) curve and the success rate at one year after surgery. Safety was assessed by comparing the postoperative complications. The number of anti-glaucoma agents, horizontal corneal diameter (HCD) and cup-to-disc ratio (C/D) of the two surgical methods were also compared.

Results: Data of 90 eyes from 61 patients were analysed. The baseline parameters of the two groups were similar. The IOP at 12 months after surgery in the VST group was 12.7 ± 4.8 mmHg, while that in the VT group was 15.8 ± 6.5 mmHg. The IOP at 6, 9 and 12 months postoperatively in the VST group was significantly lower than in the VT group (p < 0.05). Viscocanalostomy plus near-360-degree suture trabeculotomy (VST) remained a significant favourable factor for complete one-year success (93.6% versus 74.4%, p = 0.005) but not qualified one-year success (97.9% versus 88.4%, p = 0.06). The number of anti-glaucoma agents, HCD and C/D were reduced in both groups. Postoperative complications were not significantly different between the two groups.

Conclusions: In children with PCG, VST provides a more durable IOP control than VT over the one-year follow-up, with a similar safety profile.
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http://dx.doi.org/10.1111/aos.14941DOI Listing
June 2021

Correction to: Sex-Specific Transcriptome Sequencing of Zoea I Larvae and Identification of Sex-Linked Genes Using Bulked Segregant Analysis in Pacific White Shrimp Litopenaeus vannamei.

Mar Biotechnol (NY) 2021 Jun;23(3):516

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.

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http://dx.doi.org/10.1007/s10126-021-10038-3DOI Listing
June 2021

The Public Servants' Response When Facing Pandemic: The Role of Public Service Motivation, Accountability Pressure, and Emergency Response Capacity.

Healthcare (Basel) 2021 May 1;9(5). Epub 2021 May 1.

School of Economics and Management, Fuzhou University, Fuzhou 350108, China.

(1) Background: Public servants are regarded as guardians of the public interest, and their pandemic response played a vital role in controlling the spread of the epidemic. However, there is limited knowledge of the factors that influence public servants' response (PSR) when facing pandemic prevention and control tasks. (2) Methods: Based on the theory of planned behavior (TPB), models were constructed and a regression method was employed with Chinese civil servant data to investigate how PSR is influenced by public service motivation (PSM), accountability pressure (AP), and emergency response capacity (ERC). (3) Results and discussion: PSM, AP, and ERC all have a positive effect on PSR, with AP having the greatest influence, followed by PSM and ERC. The effects of PSM, AP, and ERC on PSR have group heterogeneity, which had little effect on civil servants with very low levels of PSR and the greatest impact on civil servants with medium-level PSR. Job categories of civil servants also are a factor related to PSR; PSM and AP have the strongest effects on civil servants in professional technology, and ERC has the greatest influence on administrative law enforcement. Moreover, gender, administrative level, and leadership positions also have an impact on PSR. (4) Conclusions: Based on the factors of PSR, we found at least three important aspects that governments need to consider in encouraging PSR when facing a pandemic.
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http://dx.doi.org/10.3390/healthcare9050529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147436PMC
May 2021

[Application of continuous monitoring of intracranial pressure and brain oxygen partial pressure in the treatment of patients with severe craniocerebral injury].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Apr;33(4):449-454

Department of Neurosurgery, People's Hospital of Inner Mongolia Autonomous Region, Hohhot 010017, Inner Mongolia Autonomous Region, China. Corresponding author: Zhang Ruijian, Email:

Objective: To investigate the effects of continuous monitoring intracranial pressure (ICP) and brain oxygen partial pressure (PbtO) on the prognosis of patients with severe craniocerebral injury.

Methods: A prospective randomized controlled trial was conducted. Seventy patients with severe craniocerebral injury with a Glasgow coma score (GCS) 4-8 admitted to the neurosurgical intensive care unit (NICU) of the People's Hospital of Inner Mongolia Autonomous Region from January 2017 to May 2020 were enrolled, and they were divided into ICP monitoring group and ICP+PbtO monitoring group by random number table. Patients in ICP monitoring group received ICP monitoring and were given traditional treatment of controlling ICP and cerebral perfusion pressure (CPP), the therapeutic target was ICP < 20 mmHg (1 mmHg = 0.133 kPa) and CPP > 60 mmHg. Patients in ICP+PbtO monitoring group were given ICP and PbtO monitoring at the same time, and oxygen flow was adjusted on the basis of controlling ICP and CPP to maintain the PbtO > 20 mmHg, and the therapeutic target of ICP and CPP was the same as the ICP monitoring group. ICP and PbtO values were recorded during monitoring in the two groups, the results of CPP, GCS and arterial blood gas analysis were recorded, and the prognosis at 3 months and 6 months after injury was compared by Glasgow outcome scale (GOS) score between the two groups. GOS score > 3 was considered as good prognosis. Kaplan-Meier survival curve was drawn, and the 3-month and 6-month cumulative survival rates of the two groups were analyzed. Linear regression analysis was used to further evaluate the relationship between PbtO and GOS score.

Results: Finally, a total of 70 patients with severe craniocerebral injury were enrolled in the analysis, 34 patients received ICP combined with PbtO monitoring and guided therapy, and 36 patients received ICP monitoring alone. The average ICP of ICP+PbtO monitoring group was significantly lower than that of ICP monitoring group (mmHg: 13.4±3.2 vs. 18.2±8.3, P < 0.01). Although the CPP in both groups was great than 60 mmHg, the average CPP of ICP+PbtO monitoring group was significantly higher than that of ICP monitoring group (mmHg: 82.1±10.5 vs. 74.5±11.6, P < 0.01). No significant difference was found in average GCS score or arterial partial pressure of carbon dioxide (PaCO) between the ICP+PbtO monitoring group and ICP monitoring group [GCS score: 5.3±2.3 vs. 5.2±2.2, PaCO (mmHg): 33.5±4.8 vs. 32.6±5.2, both P > 0.05]. The average arterial partial pressure of oxygen (PaO) of ICP+PbtO monitoring group was obviously higher than that of ICP monitoring group (mmHg: 228.4±93.6 vs. 167.3±81.2, P < 0.01). Compared with the ICP monitoring group, the good outcome rates of 3 months and 6 months after injury in the ICP+PbtO monitoring group were significantly higher (3 months: 67.6% vs. 38.9%, 6 months: 70.6% vs. 41.7%, both P < 0.05). Kaplan-Meier survival curve showed that the 3-month and 6-month cumulative survival rates of ICP+PbtO monitoring group were significantly higher than those of ICP monitoring group (3 months: 85.3% vs. 61.1%, Log-Rank test: χ = 5.171, P = 0.023; 6 months: 79.4% vs. 55.6%, Log-Rank test: χ = 4.511, P = 0.034). Linear regression analysis showed that PbtO was significantly correlated with GOS score at 3 months and 6 months after injury in patients with severe craniocerebral injury (r values were 0.951 and 0.933, both P < 0.01).

Conclusions: PbtO compared with ICP monitoring guiding therapy is valuable in improving the prognosis of patients with severe craniocerebral injury. It can improve the prognosis at 3-6 months after injury.
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http://dx.doi.org/10.3760/cma.j.cn121430-20201106-00700DOI Listing
April 2021

TSP50 promotes the Warburg effect and hepatocyte proliferation via regulating PKM2 acetylation.

Cell Death Dis 2021 05 20;12(6):517. Epub 2021 May 20.

Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education, Northeast Normal University, Changchun, China.

Metabolic reprogramming is a hallmark of malignancy. Testes-specific protease 50 (TSP50), a newly identified oncogene, has been shown to play an important role in tumorigenesis. However, its role in tumor cell metabolism remains unclear. To investigate this issue, LC-MS/MS was employed to identify TSP50-binding proteins and pyruvate kinase M2 isoform (PKM2), a known key enzyme of aerobic glycolysis, was identified as a novel binding partner of TSP50. Further studies suggested that TSP50 promoted aerobic glycolysis in HCC cells by maintaining low pyruvate kinase activity of the PKM2. Mechanistically, TSP50 promoted the Warburg effect by increasing PKM2 K433 acetylation level and PKM2 acetylation site (K433R) mutation remarkably abrogated the TSP50-induced aerobic glycolysis, cell proliferation in vitro and tumor formation in vivo. Our findings indicate that TSP50-mediated low PKM2 pyruvate kinase activity is an important determinant for Warburg effect in HCC cells and provide a mechanistic link between TSP50 and tumor metabolism.
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http://dx.doi.org/10.1038/s41419-021-03782-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138007PMC
May 2021

Genome Sequencing and Assembly Strategies and a Comparative Analysis of the Genomic Characteristics in Penaeid Shrimp Species.

Front Genet 2021 3;12:658619. Epub 2021 May 3.

CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Penaeid shrimp (family Penaeidae) represents one of the most economically and ecologically important groups of crustaceans. However, their genome sequencing and assembly have encountered extreme difficulties during the last 20 years. In this study, based on our previous genomic data, we investigated the genomic characteristics of four penaeid shrimp species and identified potential factors that result in their poor genome assembly, including heterozygosity, polyploidization, and repeats. Genome sequencing and comparison of somatic cells (diploid) of the four shrimp species and a single sperm cell (haploid) of identified a common bimodal distribution of K-mer depths, suggesting either high heterozygosity or abundant homo-duplicated sequences present in their genomes. However, penaeids have not undergone whole-genome duplication as indicated by a series of approaches. Besides, the remarkable expansion of simple sequence repeats was another outstanding character of penaeid genomes, which also made the genome assembly highly fragmented. Due to this situation, we tried to assemble the genome of penaeid shrimp using various genome sequencing and assembly strategies and compared the quality. Therefore, this study provides new insights about the genomic characteristics of penaeid shrimps while improving their genome assemblies.
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http://dx.doi.org/10.3389/fgene.2021.658619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126689PMC
May 2021

Purification and structure analysis of zinc-binding protein from Mizuhopecten yessoensis.

J Food Biochem 2021 06 16;45(6):e13756. Epub 2021 May 16.

School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, P.R. China.

Zn-binding protein was obtained after purification from scallops (Mizuhopecten yessoensis) using gel permeation and ion-exchange chromatography. Amino acid determination showed that the cysteine of the zinc-binding protein accounted for one-third of the total amino acids, which is a typical feature of metallothionein (MT). The spectra of Fourier Transform Infrared Spectroscopy (FTIR) and Circular Dichroism (CD) were analyzed to predict the secondary structure information of zinc-binding protein: the α-helix was 46.55%, the β-sheets was 27.07%, the random coil was 16.48%, and the β-turns was 9.89%. Using a commercial kit to measure its antioxidant activity in vitro, the result showed that it had good scavenging ability to 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH), and reducing the ability to ferrous iron ions. With the process provided by this study, zinc-binding protein can be prepared in large quantities, which is the basis for its future commercialization. PRACTICAL APPLICATIONS: According to the extraction and purification process established in this study, a large amount of zinc-bound MT from the viscera of scallops can be obtained. And the zinc-bound MT had good antioxidant activity. In addition, the yield of each purification step has been calculated. The zinc-bound MTs from scallop' viscera can be prepared in large quantities by directly using the process in this manuscript or by equal magnification of this process. In the future, large-scale production can be considered to increase the economic value of scallops' viscera.
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http://dx.doi.org/10.1111/jfbc.13756DOI Listing
June 2021

Forkhead Box Protein O1: Functional Diversity and Post-Translational Modification, a New Therapeutic Target?

Drug Des Devel Ther 2021 3;15:1851-1860. Epub 2021 May 3.

Blood Purification Center, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250011, People's Republic of China.

Forkhead box protein O1 (FoXO1) is a transcription factor involved in the regulation of a wide variety of physiological process including glucose metabolism, lipogenesis, bone mass, apoptosis, and autophagy. FoXO1 dysfunction is involved in the pathophysiology of various diseases including metabolic diseases, atherosclerosis, and tumors. FoXO1 activity is regulated in response to different physiological or pathogenic conditions by changes in protein expression and post-translational modifications. Various modifications cooperate to regulate FoXO1 activity and FoXO1 target gene transcription. In this review, we summarize how different post-translational modifications regulate FoXO1 physiological function, which may provide new insights for drug design and development.
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http://dx.doi.org/10.2147/DDDT.S305016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106445PMC
May 2021

Role of F-FDG PET/CT in the differential diagnosis of primary benign and malignant unilateral adrenal tumors.

Quant Imaging Med Surg 2021 May;11(5):2013-2018

Department of Nuclear Medicine, Chinese PLA General Hospital, Beijing, China.

Background: This retrospective study was performed to estimate the clinical role of whole-body positron emission tomography/computed tomography (PET/CT) using 2-[F] fluoro-2-deoxy-D-glucose (FDG) in the differential diagnosis of primary benign and malignant unilateral adrenal tumors.

Methods: A total of 64 patients (31 male, 33 female; age range: 3-76 years, mean: 48.5) with a confirmed unilateral adrenal tumor underwent F-FDG PET/CT examination for diagnosis and staging. The whole-body F-FDG PET/CT examination excluded metastasis, and all patients were confirmed by operation and biopsy pathology. Their clinical data and pathological results were collected. On visual analysis of PET/CT imaging, adrenal uptake was based on a three-scale grading system. The region of interest (ROI) was delineated in the liver and the renal lesion site. Standardized uptake value (SUV) measurements were determined on a standardized reconstruction, and the maximum values (SUV) of the lesion and liver were measured. The ratio of tumor to the liver was defined as T/L. Visual interpretation, SUV-receiver operating characteristics (ROC) method, and T/L-ROC method were used to analyze the diagnostic accuracy.

Results: A total of 64 lesions (48 benign, 16 malignant lesions) were detected. The visual analysis found that 100% of Grade I cases were benign, 90.9% of Grade II cases were benign, and 65.1% of Grade III cases were benign. The SUV of malignant lesions (10.0±5.8) was higher than that of benign lesions (5.4±5.3, P<0.05). The T/L was 3.39±1.79 for malignant lesions and 1.99±2.09 for benign lesions (P<0.05). In the differentiation of primary benign and malignant unilateral adrenal tumors, the sensitivity, specificity, and accuracy of the SUV-ROC method (cut-off value =5.65) were 81.25%, 72.91%, 75.00%, and the positive and negative predictive values were 50.00% and 92.11%, respectively. The sensitivity, specificity, and accuracy of the T/L-ROC method (cut-off value =1.52) were 93.73%, 62.50%, 70.31%, and the positive and negative predictive values were 46.88% and 96.77%, respectively.

Conclusions: F-FDG PET/CT improved diagnostic accuracy in differentiating primary benign and malignant unilateral adrenal tumors. There was a high negative predictive value, and for positive prediction, other tracer imaging is needed for differential diagnosis.
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http://dx.doi.org/10.21037/qims-20-875DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047365PMC
May 2021

Orchestration of plant development and defense by indirect crosstalk of salicylic acid and brassinosteorid signaling via transcription factor GhTINY2.

J Exp Bot 2021 06;72(13):4721-4743

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Salicylic acid (SA) and brassinosteroids (BRs) are well known to regulate diverse processes of plant development and stress responses, but the mechanisms by which these phytohormones mediate the growth and defense trade-off are largely unclear. In addition, little is known about the roles of DEHYDRATION RESPONSIVE ELEMENT BINDING transcription factors, especially in biotic stress and plant growth. Here, we identified a cotton (Gossypium hirsutum) APETALA2/ETHYLENE RESPONSIVE FACTOR gene GhTINY2 that is strongly induced by Verticillium dahliae. Overexpression of GhTINY2 in cotton and Arabidopsis enhanced tolerance to V. dahliae, while knockdown of expression increased the susceptibility of cotton to the pathogen. GhTINY2 was found to promote SA accumulation and SA signaling transduction by directly activating expression of WRKY51. Moreover, GhTINY2-overexpressing cotton and Arabidopsis showed retardation of growth, increased sensitivity to inhibitors of BR biosynthesis, down-regulation of several BR-induced genes, and up-regulation of BR-repressed genes, while GhTINY2-RNAi cotton showed the opposite effects. We further determined that GhTINY2 negatively regulates BR signaling by interacting with BRASSINAZOLE-RESISTANT 1 (BZR1) and restraining its transcriptional activation of the expression of INDOLE-3-ACETIC ACID INDUCIBLE 19 (IAA19). These findings indicate that GhTINY2 fine-tunes the trade-off between immunity and growth via indirect crosstalk between WRKY51-mediated SA biosynthesis and BZR1-IAA19-regulated BR signaling.
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http://dx.doi.org/10.1093/jxb/erab186DOI Listing
June 2021

Gut Microbiota Influence Lipid Metabolism of Skeletal Muscle in Pigs.

Front Nutr 2021 13;8:675445. Epub 2021 Apr 13.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Gut microbiota is recognized as a strong determinant of host physiology including fat metabolism and can transfer obesity-associated phenotypes from donors to recipients. However, the relationship between gut microbiota and intramuscular fat (IMF) is still largely unknown. Obese Jinhua pigs (JP) have better meat quality that is associated with higher IMF content than lean Landrace pigs (LP). The present study was conducted to test the contribution of gut microbiota to IMF properties by transplanting fecal microbiota of adult JP and LP to antibiotics-treated mice. Similar to JP donors, the mice receiving JP's microbiota (JM) had elevated lipid and triglyceride levels and the lipoprotein lipase activity, as well as reduced mRNA level of angiopoietin-like 4 (ANGPTL4) in the gastrocnemius muscles, compared to those in mice receiving LP's microbiota (LM). High-throughput 16S rRNA sequencing confirmed that transplantation of JP and LP feces differently reconstructed the gut microbiota in both jejunum and colon of mouse recipients. In colonic samples, we observed an elevated ratio of Firmicutes to Bacteroidetes and increased abundance of genus in JM, which were positively correlated with obesity. Furthermore, the abundance of decreased in JM, which is positively correlated with lean. Colonic concentrations of acetate ( = 0.047) and butyrate ( = 0.014) were significantly lower in JM than in LM, and consistently, the terminal genes for butyrate synthesis, butyryl CoA: acetate CoA transferase were less abundant in colonic microbiota of JM. Taken together, these gut microbiota of obese JP intrinsically promotes IMF accumulation and can transfer the properties to mouse recipients. Manipulation of intestinal microbiota will, therefore, have the potential to improve the meat quality and flavor of pigs and even to ameliorate the metabolic syndrome in human.
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http://dx.doi.org/10.3389/fnut.2021.675445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076524PMC
April 2021

Transcriptome Analysis Provides Insights into the Mechanism of Astaxanthin Enrichment in a Mutant of the Ridgetail White Prawn .

Genes (Basel) 2021 04 21;12(5). Epub 2021 Apr 21.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

A mutant of the ridgetail white prawn, which exhibited rare orange-red body color with a higher level of free astaxanthin (ASTX) concentration than that in the wild-type prawn, was obtained in our lab. In order to understand the underlying mechanism for the existence of a high level of free astaxanthin, transcriptome analysis was performed to identify the differentially expressed genes (DEGs) between the mutant and wild-type prawns. A total of 78,224 unigenes were obtained, and 1863 were identified as DEGs, in which 902 unigenes showed higher expression levels, while 961 unigenes presented lower expression levels in the mutant in comparison with the wild-type prawns. Based on Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis, as well as further investigation of annotated DEGs, we found that the biological processes related to astaxanthin binding, transport, and metabolism presented significant differences between the mutant and the wild-type prawns. Some genes related to these processes, including crustacyanin, apolipoprotein D (ApoD), cathepsin, and cuticle proteins, were identified as DEGs between the two types of prawns. These data may provide important information for us to understand the molecular mechanism of the existence of a high level of free astaxanthin in the prawn.
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http://dx.doi.org/10.3390/genes12050618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143343PMC
April 2021

Fine Needle Biopsy Versus Core Needle Biopsy Combined With/Without Thyroglobulin or BRAF 600E Mutation Assessment for Detecting Cervical Nodal Metastasis of Papillary Thyroid Carcinoma.

Front Endocrinol (Lausanne) 2021 12;12:663720. Epub 2021 Apr 12.

Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China.

Objectives: To analyze the diagnostic benefit of fine needle aspiration biopsy cytology (FNAB-C) and core needle biopsy tissue (CNB-T) with the addition of thyroglobulin (Tg) in the washout of the needle or BRAF V600E mutation assessment in assessing cervical lymph node metastasis (LNM) in papillary thyroid carcinoma.

Materials And Methods: A total of 186 lymph nodes were punctured by fine or core needle. The diagnostic performance of FNAB-C and CNB-T with Tg in the washout or BRAF V600E mutation assessment was compared.

Results: The optimal cutoff value of FNAB-Tg was 1.0 ng/ml, with an AUC of 0.976. The sensitivity and specificity of FNAB-C in predicting cervical LNM were 97.4% and 71.4%, respectively, and the addition of FNAB-Tg could contribute to a sensitivity of 100% and a specificity of 95%, but the introduction of BRAF V600E mutation assessment was associated with a decreased sensitivity of 96.3% and a decreased specificity of 50.0%. The FNAB-Tg level showed a comparable distribution in malignant lymph nodes with different TgAb statuses, serum TSH levels, and serum Tg levels. The sensitivity and specificity of CNB-T in predicting cervical LNM were 98.9% and 100%, respectively. The addition of CNB-Tg did not alter the diagnostic ability, but the introduction of BRAF V600E mutation assessment obtained the best performance, with a sensitivity of 100% and specificity of 100%.

Conclusion: The sensitivity and specificity of FNAB-C could be increased if combined with FNAB-Tg. CNB-T alone could provide satisfactory diagnostic reliability.
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http://dx.doi.org/10.3389/fendo.2021.663720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072388PMC
April 2021

Effects of Sulcus Vocalis Depth on Phonation in Three-Dimensional Fluid-Structure Interaction Laryngeal Models.

Appl Bionics Biomech 2021 8;2021:6662625. Epub 2021 Apr 8.

School of Optoelectronic Science and Engineering, Soochow University, Suzhou 215000, China.

Sulcus vocalis is an indentation parallel to the edge of vocal fold, which may extend into the cover and ligament layer of the vocal fold or deeper. The effects of sulcus vocalis depth on phonation and the vocal cord vibrations are investigated in this study. The three-dimensional laryngeal models were established for healthy vocal folds (0 mm) and different types of sulcus vocalis with the typical depth of 1 mm, 2 mm, and 3 mm. These models with fluid-structure interaction (FSI) are computed numerically by sequential coupling method, which includes an immersed boundary method (IBM) for modelling the glottal airflow, a finite-element method (FEM) for modelling vocal fold tissue. The results show that a deeper sulcus vocalis in the cover layer decreases the vibrating frequency of vocal folds and expands the prephonatory glottal half-width which increases the phonation threshold pressure. The larger sulcus vocalis depth makes vocal folds difficult to vibrate and phonate. The effects of sulcus vocalis depth suggest that the feature such as phonation threshold pressure could assist in the detection of healthy vocal folds and different types of sulcus vocalis.
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http://dx.doi.org/10.1155/2021/6662625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052148PMC
April 2021

Identification of Growth-Associated Genes by Genome-Wide Association Study and Their Potential Application in the Breeding of Pacific White Shrimp ().

Front Genet 2021 7;12:611570. Epub 2021 Apr 7.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

The Pacific white shrimp () is the most widely cultured shrimp in the world. A great attention has been paid to improve its body weight (BW) at harvest through genetic selection for decades. Genome-wide association study (GWAS) is a tool to dissect the genetic basis of the traits. In this study, a GWAS approach was conducted to find genes related to BW through genotyping 94,113 single nucleotide polymorphisms (SNPs) in 200 individuals from a breeding population. Four BW-related SNPs located in LG19 and LG39 were identified. Through further candidate gene association analysis, the SNPs in two candidate genes, and , were found to be related with the body weight of the shrimp. Marker-assisted best linear unbiased prediction (MA-BLUP) based on the SNPs in these two genes was used to estimate the breeding values, and the result showed that the highest prediction accuracy of MA-BLUP was increased by 9.4% than traditional BLUP. These results will provide useful information for the marker-assisted breeding in
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http://dx.doi.org/10.3389/fgene.2021.611570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058354PMC
April 2021
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