Publications by authors named "Xiaojun Wang"

581 Publications

Effects of intrastriatal injection of the dopamine receptor agonist SKF38393 and quinpirole on locomotor behavior in hemiparkinsonism rats.

Behav Brain Res 2021 May 1:113339. Epub 2021 May 1.

Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, People's Republic of China. Electronic address:

Dopamine (DA) in the striatum is essential to influence motor behavior and may lead to movement impairment in Parkinson's disease (PD). The present study examined the different functions of the DA D1 receptor (D1R) and DA D2 receptor (D2R) by intrastriatal injection of the D1R agonist SKF38393 and the D2R agonist quinpirole in 6-hydroxydopamine (6-OHDA)-lesioned and control rats. All rats separately underwent dose-response behavior testing for SKF38393 (0, 0.5, 1.0, and 1.5 µg/site) or quinpirole (0, 1.0, 2.0, and 3.0 µg/site) to determine the effects of the optimal modulating threshold dose. Two behavior assessment indices, the time of latency to fall and the number of steps on a rotating treadmill, were used as reliable readouts of motor stimulation variables for quantifying the motor effects of the drugs. The findings indicate that at threshold doses, SKF38393 (1.0 µg/site) and quinpirole (1.0 µg/site) produce a dose-dependent increase in locomotor activity compared to vehicle injection. The ameliorated behavioral responses to either SKF38393 or quinpirole in lesioned rats were greater than those in unlesioned control rats. Moreover, the dose-dependent increase in locomotor capacity for quinpirole was greater than that for SKF38393 in lesioned rats. These results can clarify several key issues related to DA receptors directly and may provide a basis for exploring the potential of future selective dopamine therapies for PD in humans.
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http://dx.doi.org/10.1016/j.bbr.2021.113339DOI Listing
May 2021

Identification and Expression Analysis of / Gene Family in L.

Int J Mol Sci 2021 Apr 28;22(9). Epub 2021 Apr 28.

College of Natural Resource and Environment, Northwest A&F University, Yangling 712100, China.

Slow type anion channels (SLAC/SLAHs) play important roles during anion transport, growth and development, abiotic stress responses and hormone responses in plants. However, there is few report on SLAC/SLAHs in rapeseed (). Genome-wide identification and expression analysis of / gene family members were performed in . A total of 23 / genes were identified in . Based on the structural characteristics and phylogenetic analysis of these members, the SLAC/SLAHs could be classified into three main groups. Transcriptome data demonstrated that genes were detected in various tissues of the rapeseed and could be up-regulated by low nitrate treatment in roots. BnSLAC/SLAHs were exclusively localized on the plasma membrane in transient expression of tobacco leaves. These results will increase our understanding of the evolution and expression of the SLAC/SLAHs and provide evidence for further research of biological functions of candidates in .
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http://dx.doi.org/10.3390/ijms22094671DOI Listing
April 2021

Upconversion nanoparticles modified by CuS for photothermal therapy along with real-time optical thermometry.

Nanoscale 2021 Apr 7;13(15):7161-7168. Epub 2021 Apr 7.

Department of Mathematics and Physics, Chongqing University of Posts and Telecommunications, 2 Chongwen Road, Chongqing 400065, China.

Highly effective photothermal conversion performance coupled with high resolution temperature detection in real time is urgently needed for photothermal therapy (PTT). Herein, ultra-small CuS nanoparticles (NPs) were designed to absorb on the surface of NaScF: Yb/Er/Mn@NaScF@SiO NPs to form a central-satellite system, in which the CuS NPs play the role of providing significant light-to-heat conversion ability and the Er ions in the NaScF: Yb/Er/Mn cores act as a thermometric probe based on the fluorescence intensity ratio (FIR) technology operating in the biological windows. A wavelength of 915 nm is used instead of the conventional 980 nm excitation wavelength to eliminate the laser induced overheating effect for the bio-tissues, by which Yb can also be effectively excited. The temperature resolution of the FIR-based optical thermometer is determined to be better than 0.08 K over the biophysical temperature range with a minimal value of 0.06 K at 298 K, perfectly satisfying the requirements of biomedicine. Under the radiation of 915 nm light, the CuS NPs exhibit remarkable light-to-heat conversion capacity, which is proved by photothermal ablation testing of E. coli. The results reveal the enormous potential of the present NPs for PTT integrated with real-time temperature sensing with high resolution.
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http://dx.doi.org/10.1039/d0nr09115dDOI Listing
April 2021

Silver Ion Hydrocolloid Gauze and Self-Adhesive Polyurethane Foam Dressing Combination Therapy Can Better Promote Healing of Skin Graft Donor Area Than Traditional Therapy: A Case Control Study.

Wounds 2021 04 21. Epub 2021 Apr 21.

Department of Cosmetic and Plastic Surgery, Peking Union Medical College Hospital, Beijing, China.

Background: The management of skin graft donor area has been a troublesome problem in reconstructive surgery. Currently, no guidelines exist for the management of skin graft donor areas, and the disposal methods vary from clinician to clinician.

Objective: With the goal of providing a better basis for improved patient care, the authors conducted a case control study to investigate whether a combination of silver ion hydrocolloid gauze and self-adhesive polyurethane foam dressing (AG+foam group) was effective in healing skin graft donor sites.

Materials And Methods: Forty-eight patients requiring intermediate-thickness skin graft between January 2014 and December 2015 were included in the study. Inclusion criteria included a skin graft measuring at least 40 cm2 to be harvested from the ipsilateral thigh and patient age of 14 years to 60 years. All patients were treated differently according to the experience of their own doctor. Donor sites were covered with silver ion hydrocolloid gauze and self-adhesive polyurethane foam dressing (AG+foam group, n = 23]) or petrolatum gauze, sterile gauze, and compression bandage (control group, n = 25]).

Results: Wounds healed faster (mean, 12.7 days ± 2.4 standard deviation vs 22.0 days ± 5.8; P < .001), with fewer dressing changes (median [interquartile range, IQR], 2 [IQR, 2-3] vs 4 [IQR, 2.5-5]; P = .002), and with reduced pain on first dressing change (median visual analog scale score, 5 [4-6] vs 7 [6-7.5]; P < .001) in the AG+foam group compared with the control group. The Patient and Observer Scar Assessment Scales and overall scar ratings were better in the AG+foam group than in the control group (P < .001 for all). The AG+foam group had no infections; 2 infections occurred in the control group.

Conclusions: Combined use of AG+foam dressing may enhance skin graft donor site healing, alleviate pain, and require fewer dressing changes.
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April 2021

A pilot study on ex vivo expanded autologous adipose-derived stem cells of improving fat retention in localized scleroderma patients.

Stem Cells Transl Med 2021 Apr 19. Epub 2021 Apr 19.

Department of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

In patients with localized scleroderma (LoS), facial deformity induced by subcutaneous atrophy greatly reduces life quality. Autologous fat grafting (AFG) is used for volume restoration but with low-fat retention due to various reasons. Adipose-derived stem cells (ADSCs) have shown potential effects in improving fat retention. We aimed to compare the feasibility and efficacy of improving fat retention in LoS patients among the ADSCs-assisted, the stromal vascular fraction (SVF)-assisted and conventional AFG methods. A pilot study with a 6-month follow-up among 18 LoS patients was conducted. Participants were randomly assigned into three AFG groups: conventional group, SVF-assisted group, and ADSCs-assisted group. The SVF-assisted group received SVF-assisted AFG at the SVF:fat ratio of 1:1. The ADSCs-assisted group received the mixture of ADSCs-enriched fat graft supplemented with 5 × 10 ADSCs/mL fat. Volume retention was measured by magnetic resonance imaging, and clinical photographs were taken for outcome evaluation. At sixth-month follow-up, the fat retention of ADSCs-assisted group was 49.83 ± 3.61%, significantly higher than 31.75 ± 1.73% of SVF-assisted group (P = .0004), and 21.86 ± 1.68% of the conventional group (P < .0001). A significant difference of the fat retention was also observed between the SVF-assisted and conventional group (P = .0346). No severe adverse events occurred during the procedure and follow-up. This pilot study suggests that ADSCs-assisted AFG is a safe, feasible, and attractive alternative to conventional and SVF-assisted AFG in the correction of facial atrophy of LoS patients. Future studies with large patient samples are needed for confirmation. (Chinese Clinical Trial Registry, ChiCTR1900025717).
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http://dx.doi.org/10.1002/sctm.20-0419DOI Listing
April 2021

Effects of the tight junction protein CLDN6 on cell migration and invasion in high-grade meningioma.

World Neurosurg 2021 Apr 13. Epub 2021 Apr 13.

Central Laboratory, The First People's Hospital of Yibin, Yibin 644000, Sichuan, China. Electronic address:

Background: Meningioma is a common tumor of the central nervous system, and malignant meningioma is highly aggressive and frequently recurs after surgical resection. Claudin 6 (CLDN6) is involved in cell proliferation, migration, and invasion and plays a role in maintaining tight junctions (TJs) between cells as well as obstructing the movement of cells to neighboring tissues.

Methods: In this study, we attempted to evaluate the effect of TJ protein CLDN6 expression levels on meningioma invasiveness by using silencing and overexpression constructs in both in vitro and in vivo models. The expression of CLDN6 at the mRNA and protein levels was measured using quantitative RT-PCR and western blot assays.

Results: We found that CLDN6 was expressed at higher levels in normal meningeal tissue/cell samples. Then, vectors with silenced and overexpressed CLDN6 were successfully established, and the expression levels of CLDN6 mRNA and protein in IOMM-Lee and CH157-MN cell lines were downregulated after transfection with siRNA-CLDN6 and upregulated by transfection of the entire CLDN6 sequence vector. An in vitro assay revealed that abrogation of CLDN6 expression added to the capacity of tumor migration and invasion relative to the overexpression of CLDN6. In addition to the in vitro evidence, we observed a significant increase in tumor growth and invasion-associated gene expression, including MMP-2, MMP-9, vimentin and N-cadherin, after silencing CLDN6 expression in vivo.

Conclusions: CLDN6 may play an important role in meningioma migration and invasion, and it may serve as a novel diagnostic/prognostic biomarker as well as a potential therapeutic target.
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http://dx.doi.org/10.1016/j.wneu.2021.04.005DOI Listing
April 2021

Cerebral Embolism as a Result of Facial Filler Injections: A Literature Review.

Aesthet Surg J 2021 Apr 15. Epub 2021 Apr 15.

Private practice, Singapore.

Background: With the popularity of facial filler injections, increased severe adverse events, such as cerebral embolism, have been reported.

Objectives: This article aims to summarize the clinical manifestations, and proposed mechanism of the filler-induced cerebral embolism (FICE) cases.

Methods: A literature review was performed with the search keywords including "filler injection, hyaluronic acid, fat graft, cerebral infarction/embolism, stroke, cerebrovascular infarction, disorders of consciousness, and hemiplegia".

Results: Among the 43 cases of FICE from 35 articles enrolled, 37 patients were female, and 6 were male. Twenty-nine of these patients received fat grafting, followed by 12 who received HA injection. Most of the injection sites of FICE patients were at the glabella, followed by temporal, forehead, and nasal areas. Among 30 patients receiving injection under local anesthesia, 43.33% presented with neurological symptoms during the procedure. The main symptoms were consciousness disorders and hemiplegia. Most of the embolization sites were in the middle cerebral artery, followed by frontal lobe infarction and anterior cerebral artery infarction. Three patients developed cerebral hemorrhage after embolism. Twenty-six patients presented with newly acquired vision loss, and 17 patients did not have newly acquired vision loss. The management for FICE cases included embolectomy, thrombolysis, decompressive craniectomy, antiplatelet/anticoagulant therapy and symptomatic and nutritional treatment. Nearly half of the patients recovered or improved in neurological manifestations but not the visual loss. Five patients died.

Conclusions: FICE is a severe complication following facial filler injection. Careful prevention, timely identification and treatment are crucial to decrease the morbidity and mortality of FICE.
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http://dx.doi.org/10.1093/asj/sjab193DOI Listing
April 2021

Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development.

J Sci Food Agric 2021 Apr 15. Epub 2021 Apr 15.

Department of Forest and Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver, Canada.

Background: Styrax tonkinensis is a white-flowered tree with considerable potential as a feedstock source for biodiesel production from the oily seed contained within its nutlike drupes. Transcriptome changes during oil accumulation have been previously reported, but not concurrent changes in the proteome.

Results: Using proteomic analysis of samples collected at 50, 70, 100 and 130 days after flowering (DAF), we identified 1472 differentially expressed proteins (DEPs). Based on their expression patterns, we grouped the DEPs into nine clusters and analyzed the pathway enrichment. Proteins related to starch and sucrose metabolism were most abundant at 50 DAF. Proteins involved in fatty acid (FA) biosynthesis were mainly grouped into a cluster that peaked at 70 DAF. Proteins related to protein processing in endoplasmic reticulum had two major patterns, trending either upwards or downwards, while proteins involved in amino acid biosynthesis showed more complex relationships. We identified 42 key enzymes involved in lipid accumulation during kernel development, including the acetyl-CoA carboxylase complex (ACC) and the pyruvate dehydrogenase complex (PDC). One oil body membrane protein, oleosin, continuously increased during kernel development.

Conclusion: A regulatory network of oil accumulation processes was built based on protein and available transcriptome expression data, which were in good temporal agreement. This analysis placed ACC and PDC in the center of the network, suggesting that the glycolytic provision of substrate plays a central regulatory role in FA biosynthesis and oil accumulation.
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http://dx.doi.org/10.1002/jsfa.11262DOI Listing
April 2021

Regulating the Electronic Structure and Active Sites in Ni Nanoparticles by Coating N-Doped C Layer and Porous Structure for an Efficient Overall Water Splitting.

Inorg Chem 2021 May 9;60(9):6764-6771. Epub 2021 Apr 9.

Jiangsu Key Laboratory of Green Synthetic Chemistry for Functional Materials, Department of Chemistry, School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou 221116, P. R. China.

Developing efficient and robust bifunctional electrocatalysts are in high demand for the production of hydrogen by water splitting. Engineering an electrocatalyst with a regulated electronic structure and abundant active sites is an effective way to enhance the electrocatalytic activity. Herein, N-doped C-encapsulated Ni nanoparticles (Ni@N-C) are synthesized through a traditional hydrothermal reaction, followed by pyrolyzing under an Ar/H atmosphere. The electrochemical measurements and density functional theory (DFT) calculations reveal that the electron transfer between the Ni core and the N-C shell induces the electron density redistribution on Ni@N-C, which directly promotes the adsorption and desorption of H* on the N-doped carbon (N-C) layer and thus dramatically enhances hydrogen production. Taking advantage of the porous spherical structure and the synergistic effects between Ni and N-doped carbon (N-C) layer, we obtain a Ni@N-C electrocatalyst that exhibits remarkable hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) activity with low overpotentials of 117 and 325 mV, respectively. Impressively, the assembled cell using Ni@N-C as both anode and cathode exhibits excellent activity as well as stable cyclability for over 12 h.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00595DOI Listing
May 2021

Considerations and perspectives on digestive diseases during the COVID-19 pandemic: a narrative review.

Ann Palliat Med 2021 Apr 1;10(4):4858-4867. Epub 2021 Apr 1.

Department of Gastroenterology, the First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases, Gansu Province, The First Hospital of Lanzhou University, Lanzhou, China.

Coronavirus disease 2019 (COVID-19) was initially reported in December 2019, and since then it has become a pandemic with newly confirmed cases and deaths increasing continuously. The COVID-19 pandemic has dramatically impacted the organization and execution of activities in the clinical sector. Asymptomatic infections are increasingly being identified when patients seek medical advice for non-respiratory system illnesses, particularly digestive system symptoms. This has posed a significant challenge for clinical diagnosis and treatment. Based on the clinical symptoms of patients with COVID-19 reported to date, patients with typical clinical symptoms of COVID-19 may also present with symptoms associated with the digestive system. Digestive illness symptoms in patients with COVID-19 are underscored by a bidirectional relationship between respiratory and digestive systems. Because the clinical diagnosis and treatment of digestive illnesses caused by COVID-19 have been challenging so far, we hypothesized that investigating the pathogenesis of digestive system diseases in patients with COVID-19 will provide potential novel targets for its prevention and treatment, and concurrently reduce COVID-19 virulence and socio-sanitary burden. This review summarizes the relationship between the digestive and respiratory systems in patients with COVID-19 from the perspective of the "gut-lung" axis. We discuss extant literature on the pathogenesis of COVID-19-related digestive symptoms, which may facilitate differential diagnosis and treatment of this condition.
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http://dx.doi.org/10.21037/apm-20-2124DOI Listing
April 2021

Developing a novel strategy for COPD therapy by targeting Nrf2 and metabolism reprogramming simultaneously.

Free Radic Biol Med 2021 Apr 1;169:436-445. Epub 2021 Apr 1.

Medical Imaging. the First Affiliated Hospital of Xi'an Jiaotong University. 277 West Yanta Road, Xi'an, Shaanxi, 710061, People's Republic of China. Electronic address:

Chronic obstructive pulmonary disease (COPD) is estimated to be the sixth major cause of disability, and the third main cause of death in the world by 2020. Although both inflammation and oxidative stress are well known to be the key predisposing factors in the pathogenesis of COPD, other elements, including metabolism, may also contribute to the exacerbation of the disease. However, the therapeutic approach which alters metabolism against COPD has yet been fully developed. Therefore, here we provide a novel therapeutic strategy for COPD patients. We first screened out the known nuclear factor erythroid-2-related factor 2 (Nrf2) activators, CPUY192018, which inhibits glycolysis, boosts antioxidative stress simultaneously and delivers satisfying therapeutic effect in macrophages from COPD patients and cigarette smoke extract induced COPD mice. Furthermore, we clarify that CPUY192018 not only disrupts the interaction between Kelch-like ECH-associated protein 1 (Keap1) and Nrf2, which liberates Nrf2 to activate the antioxidative pathway but also disrupt the interaction between Keap1 and actin which downregulates glycolysis, boosting the phagocytic function of alveolar macrophage in lung tissue. Taken together, CPUY192018 demonstrates notable effects on counteracting oxidative stress and reprogramming metabolism via Nrf2 activation; hence, being a raising potential therapeutic approach against COPD.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.03.039DOI Listing
April 2021

Laser Speckle Contrast Imaging for the Objective Assessment of Blood Perfusion in Keloids Treated With Dual-Wavelength Laser Therapy.

Dermatol Surg 2021 Apr;47(4):e117-e121

Plastic and Reconstructive Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Most of the widely used methods for the assessment of keloid treatment are subjective grading scales based on the opinion of an individual clinician or patient. There is a growing need for objective methods to evaluate keloid treatment.

Objective: This study aimed to evaluate the value of laser speckle contrast imaging (LSCI) as an objective method for the assessment of dual-wavelength laser therapy for keloids.

Methods: This prospective study included 21 patients with 54 keloids. All patients were treated with a combined 585-nm pulsed dye laser and 1,064 nm neodymium-doped yttrium aluminum garnet dual-wavelength laser at 4 weeks to 6 weeks intervals. Keloids were assessed using the Vancouver Scar Scale (VSS) and LSCI.

Results: The total VSS score significantly decreased after 4 sessions of treatment (p < .05). Blood perfusion in keloids as measured by LSCI was significantly reduced after treatment (p < .05). The improvement of chest keloids in terms of the total VSS score and blood perfusion was significantly greater than that of scapular keloids (p < .05). There was a positive correlation between decreased perfusion and reduced total VSS score (R2 = 0.84).

Conclusion: Blood perfusion in keloids significantly decreased after dual-wavelength laser therapy. Laser speckle contrast imaging is a promising objective method for assessing the improvement of keloids treated with laser therapy.
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http://dx.doi.org/10.1097/DSS.0000000000002836DOI Listing
April 2021

Association between renal urolithiasis after extracorporeal shock wave lithotripsy therapy and new-onset hypertension: an updated meta-analysis.

J Int Med Res 2021 Apr;49(4):3000605211002003

Department of Urology, Affiliated Hospital of Sichuan Medical College, Nanchong, China.

Objective: The long-term effect of extracorporeal shock wave lithotripsy (SWL) is still controversial. A previous meta-analysis showed no association between new-onset hypertension and entire upper urinary urolithiasis after SWL. Recently, there have been some reports on this topic. Therefore, we aimed to examine the association between new-onset hypertension and nephrolithiasis after SWL therapy.

Methods: Embase, the Cochrane Central Search Library, and PubMed were used to search for reports on new-onset hypertension and patients with nephrolithiasis after SWL. A meta-analysis of the association between new-onset hypertension and nephrolithiasis after SWL was carried out. The data of relevant research were synthesized and the relative risk was computed.

Results: Seven eligible studies were included in our meta-analysis. There was a significant association between nephrolithiasis after SWL and new-onset hypertension. The overall relative risk with a 95% confidence interval was 1.21 (1.11-1.31) in a fixed-effects model.

Conclusion: Our meta-analysis suggests an association between new-onset hypertension and patients with nephrolithiasis after SWL, which is in contrast with the finding of a previous meta-analysis.
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http://dx.doi.org/10.1177/03000605211002003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020233PMC
April 2021

Response of mercury accumulation to anthropogenic pollution in the past 1000 years based on Lake Huguangyan sediments, Southern China.

Environ Geochem Health 2021 Mar 24. Epub 2021 Mar 24.

School of Geography Sciences, South China Normal University, Guangzhou, 510631, China.

A new Pb-dated record of Hg accumulation derived from a sediment core from a Hg-enriched area in Huguangyan Lake (HGY) in South China is presented. Based on synthetic analyses of multi-proxy records including chemical composition, total organic matter, and grain-size distribution in surface sediments and nearby soil samples, it is inferred that the influx of Hg into the lake is mainly a result of atmospheric deposition, with no or minor hydroclimate-induced lithogenic input from the catchment and limited adsorption effects of organic matter and clay. Significantly enhanced anthropogenic input of Hg started in the early 1900s. Since then, several anomalies of Hg accumulation have been the results of wars or intensified economic activities in China. HGY sediments provide a rare and reliable natural archive for detecting atmospheric Hg deposition, which is closely related to anthropogenic activities.
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http://dx.doi.org/10.1007/s10653-021-00878-2DOI Listing
March 2021

False Sense of Safety: Blunt Cannulas Cause the Majority of Severe Vascular Complications in Hyaluronic Acid Injection.

Plast Reconstr Surg 2021 04;147(4):694e-695e

Division of Plastic Surgery, Peking Union Medical College Hospital, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007714DOI Listing
April 2021

Hyperbaric oxygen therapy acts as an alternative method in treating injection-induced nodules.

J Cosmet Dermatol 2021 Mar 24. Epub 2021 Mar 24.

Department of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Background: Cosmetic injection-induced nodules are tricky to handle in the clinic.

Aims: We reported a case of injection-induced nodule receiving the experimental treatment of hyperbaric oxygen therapy (HOT).

Patient: A woman presented with multiple red solid nodules on the neck after receiving mesotherapy conducted by syringe. Ultrasound examination showed multiple thickened inflammatory skin tissues on the neck. Pathological biopsy results showed epithelioid granulomas in the dermis, within which there were degenerative necrosis and foreign bodies in the center and multinucleated giant cells around. The bacteria tests remained negative. Diagnosed with foreign body granuloma, the patient rejected the resection or steroids, but willingly took the experimental treatment of HOT instead. After one month, the patient's ultrasound examination showed that the lesion's local hardness got significantly reduced, and the local blood flow increased, indicating the condition improved.

Results: Although the patient's nodule has not been eliminated, some improvements have been achieved. So far there has no case report on HOT treating injection-induced nodules in the literature.

Conclusion: HOT may be considered as a potential alternative when other treatment options cannot be implemented. More research is needed in this field.
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http://dx.doi.org/10.1111/jocd.14103DOI Listing
March 2021

Characterization of dissolved organic matter during the O-based advanced oxidation of mature landfill leachate with and without biological pre-treatment and operating cost analysis.

Chemosphere 2021 May 30;271:129810. Epub 2021 Jan 30.

CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. Electronic address:

In this study, the organic matter in an O-based advanced oxidation process (AOP) for treating raw leachate (RL) and bio-treated leachate (BTL) was characterized. The optimal conditions for COD removal in RL and BTL treatment were as follows: initial pH of 6.0 and HO dosage of 9 mL 30% HO L leachate, and initial pH of 12 without HO addition, respectively. HO addition had little influence on COD removal in the BTL treatment as sufficient hydroxyl radicals may not be produced at extremely high pH levels. The differences in the alkalinity between RL and BTL caused differences in the optimum pH of the AOPs. Overall, the initial pH more affected COD removal than the HO dosage. O-based AOP converted organics with high molecular weight fractions into low ones. Meanwhile, it preferentially degraded hydrophobic substances over hydrophilic substances. The organic matter in the BTL contained more refractory and hydrophobic fractions; therefore, higher COD removal was achieved in the treatment of RL. The organics in the treatment of RL and BTL were identified by excitation-emission matrix spectroscopy combined with parallel factor analysis, and their degradation decreased in the following order: terrestrial humic-like > microbial humic-like > combination of tryptophan and humic-like components. O-based AOP significantly enhanced biodegradability. According to the economic analysis results, as an intermediate treatment, O-based AOP is a cost-effective strategy of ensuring that leachate effluent meets the discharge standards, with the lowest operating cost of $4.62 m. This study provides a reference for the application of O-based AOP in full-scale landfill leachate treatment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129810DOI Listing
May 2021

Correction for Yin et al., "Equine Tetherin Blocks Retrovirus Release and Its Activity Is Antagonized by Equine Infectious Anemia Virus Envelope Protein".

J Virol 2021 Feb 24;95(6). Epub 2021 Feb 24.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Harbin, China

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http://dx.doi.org/10.1128/JVI.02163-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8094952PMC
February 2021

Chemical sectioning fluorescence tomography: high-throughput, high-contrast, multicolor, whole-brain imaging at subcellular resolution.

Cell Rep 2021 Feb;34(5):108709

Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China. Electronic address:

A thorough neuroanatomical study of the brain architecture is crucial for understanding its cellular compositions, connections, and working mechanisms. However, the fine- and multiscale features of neuron structures make it challenging for microscopic imaging, as it requires high contrast and high throughput simultaneously. Here, we propose chemical sectioning fluorescence tomography (CSFT) to solve this problem. By chemically switching OFF/ON the fluorescent state of the labeled proteins (FPs), we light only the top layer as thin as submicron for imaging without background interference. Combined with the wide-field fluorescence micro-optical sectioning tomography (fMOST) system, we have shown multicolor CSFT imaging. We also demonstrate mouse whole-brain imaging at the subcellular resolution, as well as the power for quantitative acquisition of synaptic-connection-related pyramidal dendritic spines and axon boutons on the brain-wide scale at the complete single-neuron level. We believe that the CSFT method would greatly facilitate our understanding of the brain-wide neuron networks.
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http://dx.doi.org/10.1016/j.celrep.2021.108709DOI Listing
February 2021

Enhanced lithium storage performance of porous Si/C composite anodes using a recrystallized NaCl template.

Dalton Trans 2021 Mar;50(8):2815-2823

Industrial Training Center, Guangdong Polytechnic Normal University, Guangzhou 510665, China.

Silicon (Si) has recently aroused great interest as a promising anode material for lithium-ion batteries with high energy density due to its high theoretical capacity. However, the application of Si remains a great challenge owing to its extremely large volume change during cycling, thus resulting in dramatic capacity fading. Herein, a novel structure design of the porous Si/C composite with Si nanoparticles embedded in the carbon nanosheets has been successfully achieved by using a recrystallized NaCl template with appropriate particle size. The outermost sheet-like carbon coating can improve the electronic conductivity and contribute to the formation of a more stable solid-electrolyte interphase layer, while the inner void space effectively buffers the volume expansion of Si during the lithiation process. In addition, only a structure with Si particles anchored on the surface of carbon nanosheets has been obtained by using a commercial NaCl template with large particle size, confirming the effective regulation of the NaCl template in the microstructure and thus the electrochemical properties of the Si/C composites. As expected, benefiting from the combination of the outermost carbon coating and recrystallized NaCl-derived porous structure, the as-obtained Si/C composite demonstrates attractive cycling stability and rate performance as an anode material for lithium-ion batteries.
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http://dx.doi.org/10.1039/d0dt03911jDOI Listing
March 2021

Time-resolved characteristics and production pathways of simulated landfilling NO emission under different oxygen concentrations.

Environ Int 2021 04 30;149:106396. Epub 2021 Jan 30.

Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

Nitrous oxide (NO), an important greenhouse gas, is emitted from landfill reservoirs, especially in the working face, where nitrification and denitrification occur under different O concentrations. In order to explore the effects of O concentration on NO emissions and production pathways, the production of NO from simulated fresh waste landfilling under 0%, 5%, 10%, and 21% (vol/vol) O concentrations were examined, and N isotopes were used as tracers to determine the contributions of nitrification (NF), heterotrophic denitrification (HD), and nitrification-coupled denitrification (NCD) to NO production over a 72-h incubation period. Equal amounts of total nitrogen consumption occurred for all studied O concentration and the simulated waste tended to release more NO under 0% and 21% O. Heterotrophic denitrification was the main source of NO release at the studied oxygen concentrations, contributing 90.51%, 69.04%, 80.75%, and 57.51% of NO under O concentrations of 0%, 5%, 10%, and 21%, respectively. Only denitrification was observed in the simulated fresh waste when the oxygen concentration of the bulk atmosphere was 0%. The nitrate reductase (nirS)-encoding denitrifiers in the simulated landfill were also studied and significant differences were observed in the richness and diversity of the denitrifying community at different taxonomic levels. It was determined that optimising the O content is a crucial factor in NO production that may allow greenhouse gas emissions and N turnover during landfill aeration to be minimised.
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http://dx.doi.org/10.1016/j.envint.2021.106396DOI Listing
April 2021

Aesthetic and therapeutic outcome of fat grafting for localized Scleroderma treatment: From basic study to clinical application.

J Cosmet Dermatol 2021 Jan 23. Epub 2021 Jan 23.

Department of Plastic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Background: Localized scleroderma (LoS) is a rare autoimmune disease characterized by skin fibrosis and subcutaneous tissue atrophy, resulting in aesthetic impairment on patients. Fat grafting has been used to treat LoS patients, achieving aesthetic and therapeutic improvement.

Aims: This article summarized the epidemiology and pathophysiology of LoS and the current progress and thorny questions of basic and clinical research on fat grafting treating LoS.

Methods: The literature of the last 20 years concerning fat grafting of treating LoS was reviewed.

Results: Fat grafting has been proved to produce aesthetic and therapeutic outcomes on LoS patients, including the improvement of soft tissue atrophy, skin fibrosis and pigmentation. Due to the inflammatory microenvironment of scleroderma, however, fat grafting still faces many difficulties, such as low fat retention. Novel fat grafting methods in order to supplement the deficiency of adipose-derived stem cells and improve fat retention in LoS groups have been proposed whose effectiveness and feasibility is still needed further study.

Conclusion: Currently, fat grafting has been regarded as an effective treatment with a combination of aesthetic and therapeutic outcomes on LoS patients.
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http://dx.doi.org/10.1111/jocd.13941DOI Listing
January 2021

Association between p73 G4C14-to-A4T14 polymorphism and lung cancer risk: A systematic review and meta-analysis.

Clin Respir J 2021 Jan 23. Epub 2021 Jan 23.

Radiology Intervention Department, Anhui Chest Hospital, Hefei, China.

Objective: This study was conducted to evaluate the relationship between the p73 G4C14-to-A4T14 polymorphism (hereafter, G4C14-to-A4T14) and lung cancer risk.

Methods: The studies on the relationship between G4C14-A4T14 and lung cancer risk published as of November 5, 2018, were comprehensively searched in PubMed, Embase, the Cochrane Library, the Chinese Wanfang database, China National Knowledge Infrastructure (CNKI), and China Biology Medicine (CBM). The last update was on May 24, 2019. Statistical analysis was performed using Stata 12.0.

Results: The association between G4C14-A4T14 and lung cancer risk was analyzed in nine studies. The findings indicate no association between G4C14-to-A4T14 and lung cancer risk (allele model: OR = 0.90, 95% CI: 0.73-1.11, I  = 86.0%, P = .330; dominant model: OR = 0.93, 95% CI: 0.74-1.17, I  = 82.6%, P = .551; recessive model: OR = 0.75, 95% CI: 0.50-1.13, I  = 75.2%, P = .165; homozygote model: OR = 0.74, 95% CI: 0.47-1.17, I  = 79.6%, P = .199; heterozygote model: OR = 0.98, 95% CI: 0.80-1.21, I  = 75.8%, P = .879). The heterogeneity between subgroups by cancer types and genotyping method was significantly reduced. After the deletion of suspected duplicates, no association was found between G4C14-to-A4T14 and lung cancer susceptibility.

Conclusion: Our meta-analysis confirms that G4C14-to-A4T14 is not significantly related to lung cancer risk.
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http://dx.doi.org/10.1111/crj.13335DOI Listing
January 2021

The Development and Initial Validation of PUMC Localized Scleroderma Facial Aesthetic Index: A Pilot Study.

Aesthetic Plast Surg 2021 Jan 15. Epub 2021 Jan 15.

Department of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Background: Localized scleroderma (LoS) is an autoimmune connective tissue disorder leading to serious long-term aesthetic impairment on patients. Objective evaluation methods are badly needed to facilitate the evaluation of the surgical treatment on individual patients and clinical studies.

Objective: To develop and assess the reliability and validity of Peking Union Medical College LoS facial aesthetic index (PUMC LoSFAI).

Methods: Twelve experts devoted their time and resources in the development and validation. LoS patients in the stable phase were recruited. Reliability and validity was then assessed. LoS patients were evaluated by two plastic surgeons using PUMC LoSFAI and LoS skin damage index (LoSDI). The PUMC LoSFAI comprises 4 domains for the local assessment (surface area of lesion, dyspigmentation, skin thickness and soft tissue atrophy) and 3 domains for the overall assessment (facial symmetry, proportion and profile) to describe LoS facial aesthetic impairment. Face-Q was completed by patients at each visit.

Results: Thirty-two LoS patients had 96 visits, during which 138 lesions were assessed. PUMC LoSFAI and 7 domains demonstrated substantial to excellent inter- and intra-rater reliability (ICC 0.995, κ 0.72-0.91, r 0.85-0.99, respectively). Seven domains considered to be important to extremely important variables (mean rank 3.2-3.8) had high I-CVI (> 0.78) and S-CVI (0.93). PUMC LoSFAI correlated excellently with LoSDI (r = 0.933, P < 0.001), and correlated fairly with Face-Q (r = - 0.399, P = 0.001).

Conclusions: PUMC LoSFAI was developed and evaluated to play as a tool of aesthetic impairment assessment for LoS patients, which may facilitate the evaluation of the treatment on individual patients and clinical studies. PUMC LoSFAI demonstrated high reliability and validity, and further study in larger patient samples is needed to confirm these preliminary findings.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-020-02111-4DOI Listing
January 2021

Unexpectedly high mutation rate of a deep-sea hyperthermophilic anaerobic archaeon.

ISME J 2021 Jan 15. Epub 2021 Jan 15.

Simon F. S. Li Marine Science Laboratory, School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.

Deep-sea hydrothermal vents resemble the early Earth, and thus the dominant Thermococcaceae inhabitants, which occupy an evolutionarily basal position of the archaeal tree and take an obligate anaerobic hyperthermophilic free-living lifestyle, are likely excellent models to study the evolution of early life. Here, we determined that unbiased mutation rate of a representative species, Thermococcus eurythermalis, exceeded that of all known free-living prokaryotes by 1-2 orders of magnitude, and thus rejected the long-standing hypothesis that low mutation rates were selectively favored in hyperthermophiles. We further sequenced multiple and diverse isolates of this species and calculated that T. eurythermalis has a lower effective population size than other free-living prokaryotes by 1-2 orders of magnitude. These data collectively indicate that the high mutation rate of this species is not selectively favored but instead driven by random genetic drift. The availability of these unusual data also helps explore mechanisms underlying microbial genome size evolution. We showed that genome size is negatively correlated with mutation rate and positively correlated with effective population size across 30 bacterial and archaeal lineages, suggesting that increased mutation rate and random genetic drift are likely two important mechanisms driving microbial genome reduction. Future determinations of the unbiased mutation rate of more representative lineages with highly reduced genomes such as Prochlorococcus and Pelagibacterales that dominate marine microbial communities are essential to test these hypotheses.
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http://dx.doi.org/10.1038/s41396-020-00888-5DOI Listing
January 2021

Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water.

Aesthet Surg J 2021 Jan 11. Epub 2021 Jan 11.

Department of Plastic Surgery, Pekiong Union Medical College Hospital, Pekiong Union Medical College, Beijing, China.

Background: Adipose-derived stem cells (ASCs) are considered promising cells for skin rejuvenation. However, whether the angiogenetic effect of ASCs plays an important role in the treatment of aging skin and its influence on skin tissue remain elusive.

Objectives: The present study evaluated the effect of ASCs on angiogenesis and local tissue water (LTW) in the aging skin of nude mice.

Methods: Twelve nude mice were randomly divided into a UVB-induced photoaging group and a natural aging group. After the mouse model was established, ASCs and PBS were separately injected into the dorsal skin of the mice. Blood perfusion and LTW were measured. After 7 weeks, mice were sacrificed, and skin samples were collected to measure the thickness of the dermis, density of the capillaries and expression of angiogenic growth factors.

Results: ASC therapy significantly increased the thickness of the dermis, the number of capillaries, and the expression of some angiogenic growth factors (VEGF, IGF-1, and EGF). At 7 weeks after injection, blood perfusion was significantly higher on the ASCs-injection side than on the PBS-injection side. The LTW was increased in the PBS-injection side of photoaging mice. The ASCs-injection side showed no significant changes over time.

Conclusions: ASCs increased dermal thickness, promoted angiogenesis and reduced LTW in the skin of photoaging mice, providing a potential clinical therapy for skin rejuvenation.
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http://dx.doi.org/10.1093/asj/sjab001DOI Listing
January 2021

Somatic Symptom Disorder Patients Seeking Aesthetic Procedures: Tricky Situations in Clinical Practice.

Plast Reconstr Surg Glob Open 2020 Dec 21;8(12):e3319. Epub 2020 Dec 21.

Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

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http://dx.doi.org/10.1097/GOX.0000000000003319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787341PMC
December 2020

Implication of Ataxia-Telangiectasia-mutated kinase in epithelium-mesenchyme transition.

Carcinogenesis 2021 Apr;42(4):640-649

Key Laboratory of Birth Defects and Related Diseases of Women and Children (Ministry of Education), Department of Gynecology, West China Second University Hospital, Sichuan University, Chengdu, PR China.

Impairment of genome instability drives the development of cancer by disrupting anti-cancer barriers. Upon genotoxic insults, DNA damage responsive factors, notably ATM kinase, is crucial to protect genomic integrity while promoting cell death. Meanwhile, cytotoxic therapy-inducing DNA lesions is double-edged sword by causing cancer metastasis based on animal models and clinical observations. The underlying mechanisms for the procancer effect of cytotoxic therapies are poorly understood. Here, we report that cancer cells subjected to cytotoxic treatments elicit dramatic alteration of gene expression controlling the potential of epithelium-mesenchyme transition (EMT). Resultantly, EMT-dependent cell mobility is potently induced upon DNA damage. This stimulation of EMT is mainly Ataxia-Telangiectasia-mutated (ATM)-dependent, as the chemical inhibitor specifically inhibiting ATM kinase activity can suppress the EMT gene expression and thus cell mobility. At last, we show that cancer cells with ATM activation display increased metastatic potential in ovarian cancer tissues. Taken together, we reveal a novel role of ATM in promoting metastatic potential of cancer cells by favoring EMT gene expression.
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http://dx.doi.org/10.1093/carcin/bgab002DOI Listing
April 2021

Amniotic Fluid Transplantation Alleviates Hematopoietic Deficits in Experimental Rat Aplastic Anemia.

Ann Transplant 2021 Jan 8;26:e928047. Epub 2021 Jan 8.

Department of Basic Medical, Hebei Xinglong Institute of Pharmacological and Medical Science, Shijiazhuang, Hebei, China (mainland).

BACKGROUND Hematopoietic stem cell (HSC) transplantation is the most effective therapy for hematopoietic impairment. However, maintenance and self-renewal of HSCs in culture is still a central focus of HSC research. It is known that amniotic fluid contains a heterologous population of stem cells (AFSCs) and nutrients as well as various types of growth factors. We hypothesize that AFSCs may be expanded in vitro in pure amniotic fluid. MATERIAL AND METHODS Amniotic fluid with transparent appearance was harvested at embryo age of 13.5-15.5 days in rats and was placed in a cell culture CO₂ incubator. The cell number in the amniotic fluid was counted before and after culture of amniotic fluid. Then, the effect of amniotic fluid transplantation on 5-fluorouracil combined with busulfan induced-rat aplastic anemia was investigated. RESULTS We found that after a short time (about 30 min) culture, the number of AFSCs expanded more than 100-fold. Flow cytometry showed that there was a population of cells expressing hematopoietic markers CD45 and CD34 in addition to a higher proportional of mesenchymal stem cells (MSCs) markers CD29 and CD90. Transplantation of the expanded AFSCs possessed significant therapeutic effect in toxic chemicals induced-aplastic anemia rats, manifested by decreasing animal mortality and alleviating the reduction of the 3 lineages of hematopoietic cells in blood. CONCLUSIONS Our observation for the first time demonstrates that amniotic fluid is an excellent medium for stem cells to maintain "stemness" proliferation and provides novel evidence to support the potential use of in vitro-expanded AFSCs for the treatment of hematopoietic disorders.
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http://dx.doi.org/10.12659/AOT.928047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802395PMC
January 2021

Adhesive Bacteria in the Terminal Ileum of Children Correlates With Increasing Th17 Cell Activation.

Front Pharmacol 2020 30;11:588560. Epub 2020 Nov 30.

Gastrointestinal Lab, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child, National Children's Regional Medical Center, Hangzhou, China.

Humans and symbiotic bacteria are interdependent and co-evolved for millions of years. These bacteria communicate with human hosts in the gut in a contact-independent metabolite. Because most intestinal bacteria are non-adhesive, they do not penetrate the mucus layer and are not directly in contact with epithelial cells (ECs). Here, we found that there are adhesive bacteria attached to the Children's terminal ileum. And we compared the immune factors of non-adhesive bacteria in the children ileum with adhesive bacteria as well. Stimulated Th17 cell associated with adherent bacteria in the ileum ECs. SIgA responses are similar to those roles in mouse experiments. Immunohistochemical analysis confirmed that the expression of , and protein increased in Th17 cells. Finally, we used 16S rRNA genes 454 pyrosequencing to analyze the differences in bacterial communities between adhesive and non-adhesive bacteria in the ileum. Ileum with adherent bacteria demonstrated increased mucosa-related bacteria, such as , and . We believe that adhesive bacteria in children's terminal ileum associated with an increased Th17 cell activation and luminal secretory IgA. Adhesive bacteria very closely adhere to terminal ileum of children. They may play important role in human gut immunity and Crohn's disease.
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http://dx.doi.org/10.3389/fphar.2020.588560DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774322PMC
November 2020