Publications by authors named "Xiaojun Sun"

87 Publications

Potential Synergy Mechanism of Processing Methods for the Basic Remedies of Qixue Shuangbu Prescription Based on Integrated Metabolomics Strategy and Network Pharmacology Study.

J Chromatogr Sci 2021 May 10. Epub 2021 May 10.

Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, PR China.

Qixue Shuangbu Prescription (QSP) is a famous traditional Chinese medicine (TCM) formula widely used for the treatment of chronic heart failure (CHF). Previous clinical studies have found that the efficacy of processed QSP has been significantly enhanced in the treatment of CHF. However, the synergistic mechanisms of processed QSP to enhance the treatment of CHF are still unclear. Generally, the changes in clinical effects mainly result from the variations of inside chemical basis caused by the TCM processing procedure. In this study, we developed a network pharmacology-integrated metabolomics strategy to clarify the difference of the effective compounds between crude and processed QSP, and further explain the mechanism of processed QSP to produce a synergistic effects. As a result, 69 different compounds were successfully screened, identified, quantified and verified as the most potential marker compounds. These different chemical components may play an anti-CHF and enhance the therapeutic effect through 52 action pathways such as estrogen signaling pathway, ubiquitin mediated proteolysis, protein processing in endoplasmic reticulum, etc. This study revealed that the proposed network pharmacology-integrated metabolomics strategy was a powerful tool for explaining the mechanism of synergistic action in the processing of QSP, further controlling the quality and understanding the processing mechanism of TCM formulae.
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http://dx.doi.org/10.1093/chromsci/bmab053DOI Listing
May 2021

Interleukin-12 exacerbates symptoms in an MRL/MpJ-Faslpr mouse model of systemic lupus erythematosus.

Exp Ther Med 2021 Jun 15;21(6):627. Epub 2021 Apr 15.

Institute of Acute Disorders of Traditional Chinese Internal Medicine, The First Clinical College of Nanjing University of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China.

Interleukin (IL)-12 modulates the generation and function of a variety of immune cells and serves an important role in the pathogenesis of autoimmune diseases. However, the precise role of IL-12 in the pathogenesis of systemic lupus erythematosus (SLE) remains to be elucidated. In the present study, the serum levels of IL-12 in patients with SLE were determined using an ELISA. The association between serum levels of IL-12 and clinical and laboratory indices, specifically, disease activity and complement 3, were analyzed. Recombinant IL-12 or an anti-IL-12 antibody was used to treat the MRL/MpJ-Fas mouse model of systemic lupus erythematosus. The glomerulonephritis and inflammatory cell infiltration was examined to evaluate histological changes using hematoxylin and eosin and Periodic acid-Schiff staining. Serum creatinine and proteinuria were used to determine renal function. The levels of anti-double stranded DNA and anti-nuclear autoantibodies were assessed. The results demonstrated that serum levels of IL-12 were markedly increased in patients with SLE compared with controls and in lupus model mice in comparison with control mice. The serum levels of IL-12 increased with disease severity in patients with SLE. SLE-like symptoms were exacerbated in lupus model mice treated with exogenous IL-12. However, SLE-like symptoms were ameliorated in lupus model mice treated with an anti-IL-12 antibody. The present results demonstrated that IL-12 aggravated SLE and anti-IL12 antibodies ameliorated SLE. The present data suggest that blocking IL-12 may be a beneficial therapeutic strategy to halt the progression of lupus nephritis.
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http://dx.doi.org/10.3892/etm.2021.10059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082580PMC
June 2021

Manipulating Electrical Properties of Silica-Based Materials via Atomic Oxygen Irradiation.

ACS Appl Mater Interfaces 2021 Apr 24;13(13):15344-15352. Epub 2021 Mar 24.

Center of Advanced Lubrication and Seal Materials, Northwestern Polytechnical University, Xi'an 710072, P. R. China.

Regulated triboelectrification has attracted considerable research attention due to its potential applications in harvesting energy and importance in antistatic protection. Irradiation is an effective and stable modification method due to its adjustable and uniform irradiation parameters. Moreover, atomic oxygen (AO) irradiation is an important component in the low earth orbit, which is a considerable factor for promoting triboelectric nanogenerators (TENGs) in the outer space. AO irradiation was utilized to manipulate the surface structure and chemical composition to regulate electrical properties. AO irradiation can increase electron-donating groups and enhance electrical positivity of polydimethylsiloxane (PDMS) films due to the transition from Si-C bonds to Si-O bonds. Therefore, different trends of polytetrafluoroethylene (PTFE) and polystyrene (PS) were caused by their TENG composition with irradiated PDMS. Tribocharge cross-over polarity and charge generation were prevented completely in PS- and PDMS-based TENGs by adjusting the irradiation time to 4.1 h. Short-circuit current enhanced from 5 to 22 μA and the output voltage increased from 160 to 760 V when PDMS films in PTFE- and PDMS-based TENGs were subjected to AO irradiation for 6 h. This study demonstrated that AO irradiation can manipulate triboelectric properties of silica-based materials, which are potential components for harvesting energy and preventing electrostatic hazard in the outer space.
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http://dx.doi.org/10.1021/acsami.1c02259DOI Listing
April 2021

Efficacy of Nucleotide/Nucleoside Analogues and Hepatitis B Immunoglobulin Therapy in Blocking Mother-to-Child Transmission of Hepatitis B in an Eastern Chinese Group.

Infect Dis Obstet Gynecol 2020 17;2020:4305950. Epub 2020 Dec 17.

Wuxi Shenrui Bio-Pharmaceuticals Co., Ltd., R&D Department, Wuxi, Jiangsu 214000, China.

The objective of this study was to investigate the efficacy and potential side-effects of nucleotide/nucleoside analogues and hepatitis B immunoglobulin injection of newborns in blocking mother-to-child transmission of hepatitis B virus in the middle and late pregnancy period. 238 cases of enrolled pregnant women were divided into the Telbivudine group, the Tenofovir group, the Lamivudine group, and the hepatitis B immunoglobulin (HBIG) group. Enrolled patients received corresponding therapies. Clinical and laboratory data were collected. Results showed that the levels of HBV DNA of the enrolled pregnant women in the Telbivudine, Tenofovir, and Lamivudine groups decreased rapidly after 12 weeks of drug intervention compared with those in the control. HBsAg positive rate in newborns and in children 24 weeks after birth was 0/60, 0/60, 0/60, 3/30, and 11/28 in the Telbivudine, Tenofovir, Lamivudine, HBIG, and control groups, respectively. No significant side-effects were identified after following up to 12 months after birth. Our results show that routine HBV vaccine plus HBIG injections is insufficient in blocking mother-to-child HBV transmission. Administration of nucleotide/nucleoside analogues or HBIG at pregnancy is suggested to maximize the blocking of vertical HBV transmission.
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http://dx.doi.org/10.1155/2020/4305950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7759418PMC
December 2020

Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide.

Anal Chem 2021 01 28;93(3):1433-1442. Epub 2020 Dec 28.

National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao 266237, China.

Glycosaminoglycans (GAGs) contribute to the treatment of many human diseases, especially in the field of thrombosis, because of their anticoagulant activity. GAGs interrupt the coagulation process by interacting with multiple coagulation factors through defined sequences within their linear and negatively charged chains, which are not fully elucidated. Numerous methods have been developed to characterize the structure of pharmaceutical GAGs, including or administered heparin and orally administered sulodexide. However, most currently available methods only focus on the oligosaccharide portion or analyze the whole mixture because longer-chain polysaccharides are extremely difficult to resolve by chromatographic separation. We have established two novel electrophoresis-mass spectrometry methods to provide a panoramic view of the structures of pharmaceutical GAGs. In the first method, an in-gel digestion procedure was developed to recover GAGs from the polyacrylamide gels, while in the second method, a strong anion exchange ultrafiltration procedure was developed to extract multiple GAG species from the agarose gels. Both procedures are compatible with liquid chromatography-tandem mass spectrometry, and structural information, such as disaccharide composition and chain length, can be revealed for each GAG fraction. The applications of these two methods on analysis of two different GAG drugs, heparin and sulodexide, were demonstrated. The current study offers the first robust tool to directly elucidate the structure of larger GAG chains with more biological importance rather than obtaining a vague picture of all chains as a mixture, which is fundamental for better understanding the structure-activity relationship and quality control of the GAG drugs.
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http://dx.doi.org/10.1021/acs.analchem.0c03330DOI Listing
January 2021

Anchoring Au(111) on a Bismuth Sulfide Nanorod: Boosting the Artificial Electrocatalytic Nitrogen Reduction Reaction under Ambient Conditions.

ACS Appl Mater Interfaces 2020 Dec 2;12(50):55838-55843. Epub 2020 Dec 2.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.

Electrocatalytic nitrogen reduction reaction (NRR), as a green and sustainable method for ammonia synthesis, has become one of the candidates to substitute industrial Haber-Bosch ammonia synthesis in the near future. In this work, gold nanoparticles (Au NPs) were successfully anchored on bismuth sulfide nanorods (BiS NRs), which acted as highly efficient electrocatalytic NRR catalysts. The N-philic nature of Bi and the unique mutual coordination of Au-S-Bi can greatly promote the nitrogen adsorption and form the intermediate product NH*, achieving a boosted improvement in the NRR activity through a continuous hydrogenation reaction. Definitely, the as-synthesized Au(111)@BiS nanorod catalyst exhibits an excellent NH generation rate of 45.57 μg h mg with a faradic efficiency of 3.10% at -0.8 V vs reversible hydrogen electrode. High stability and reproducibility are also demonstrated throughout the electrocatalytic NRR process. Density functional theory calculations were performed to further understand the NRR catalytic mechanism on the Au(111)@BiS nanorods catalyst.
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http://dx.doi.org/10.1021/acsami.0c15987DOI Listing
December 2020

Behavioural and ERP evidence of the self-advantage of online self-relevant information.

Sci Rep 2020 11 25;10(1):20515. Epub 2020 Nov 25.

School of Psychology, Central China Normal University, Wuhan, 430079, China.

The present study examined whether individuals experienced the same cognitive advantage for online self-relevant information (nickname) as that experienced for information encountered in real life (real name) through two experiments at both the behavioural and neural levels (event-related potential, ERP). The results indicated that individuals showed the same cognitive advantage for nicknames and real names. At the behavioural level, a nickname was detected as quickly as the real name, and both were detected faster than a famous name; at the neural level, the P300 potential elicited by one's nickname was similar to that elicited by one's real name, and both the P300 amplitudes and latencies were larger and more prolonged than those elicited by other name stimuli. These results not only confirmed the cognitive advantage for one's own nickname and indicated that this self-advantage can be extended to online information, but also indicated that the virtual self could be integrated into the self and further expanded individuals' self-concept.
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http://dx.doi.org/10.1038/s41598-020-77538-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7688976PMC
November 2020

Oral Infection Caused by Non-O1/Non-O139 in a Patient with Esophageal Cancer Undergoing Esophagectomy and Chemoradiotherapy: A Case Report.

Infect Drug Resist 2020 3;13:3923-3927. Epub 2020 Nov 3.

Department of Clinical Laboratory, The First Affiliated Hospital of Shandong First Medical University, Jinan, People's Republic of China.

Non-O1/non-O139 (NOVC) are increasingly being recognized as causes of sporadic cases of gastroenteritis and extra-intestinal invasive infections, such as bacteremia as well as skin and wound infections in immunosuppressed hosts. However, oral infections caused by these microorganisms have rarely been reported. We present a case of oral infection caused by NOVC in a patient undergoing chemoradiotherapy after esophagectomy for esophageal cancer. The patient recovered well after antibiotic treatment. The isolate from the patient was screened for phenotypic and genetic characteristics with reference to their major virulence genes. Our report provides supporting evidence for oral infection due to NOVC in a patient with esophageal cancer and suggests that some putative accessory virulence factors may be crucial in the pathogenicity of this strain. To the best of our knowledge, this is the first documented case of oral infection due to NOVC.
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http://dx.doi.org/10.2147/IDR.S274077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648562PMC
November 2020

Construction of a hydrophobic magnetic aerogel based on chitosan for oil/water separation applications.

Int J Biol Macromol 2020 Dec 18;165(Pt B):1869-1880. Epub 2020 Oct 18.

China Petrochemical Corporation (Sinopec Group), Beijing 100728, China; Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.

Hydrophobic/oleophilic absorbents have been largely studied and used in recovering spilled oil. However, they still suffer from several drawbacks and two of them are poor biocompatibility and hard to thoroughly rinse. In order to address these problems, here a hydrophobic magnetic chitosan-based aerogel is fabricated via electrostatic interactions between chitosan (CS), itaconic acid (IA) and FeO nanoparticles and dip-coating in ethanol solution of Candelilla wax (CW). Due to the interconnected porous structure of chitosan-based aerogel, the magnetism of FeO nanoparticles and the hydrophobicity of CW, the prepared aerogel exhibits high absorption capacities (from 17.7 to 43.8 g/g) towards various types of organic liquids, excellent magnetic controllability with saturation magnetization of 15.93 emu/g and good water repellency with water contact angle (WCA) of 147.9°. In addition, the aerogel can also continuously separate immiscible oil/water mixtures and water-in-oil emulsions as the form of filter. More significantly, the absorbed organic liquids can be completely recovered by simply placing the aerogel in water solution of IA at 75 °C, which can avoid cleaning agent consumption. As a consequence, this renewable, biodegradable and eco-friendly oil scavenger presents a bright prospect in practical applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.10.068DOI Listing
December 2020

A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering.

Cell Mol Immunol 2020 Sep 22. Epub 2020 Sep 22.

State Key Laboratory of Membrane Biology, School of Medicine, Tsinghua University, 100084, Beijing, China.

Rheumatoid arthritis (RA) is exacerbated by TNF-alpha signaling. However, it remains unclear whether TNF-α-activated TNFR1 and TNFR2 are regulated by extracellular factors. Here, we showed that soluble glycosylated interleukin-17 receptor D (sIL-17RD), which was produced by proteolytic cleavage, enhanced TNF-α-induced RA. We revealed that IL-17RD shedding was induced by the proteolytic enzyme TACE and enhanced by TNF-α expression in macrophages. Intriguingly, sIL-17RD was elevated in the sera of arthritic mice and rats. Recombinant sIL-17RD significantly enhanced the TNF-α-induced proinflammatory response by promoting TNF-α-TNFR-sIL-17RD complex formation and receptor clustering, leading to the accelerated development of collagen-induced arthritis. Our observations revealed that ectodomain shedding of IL-17RD occurred in RA to boost the TNF-α-induced inflammatory response. Targeting sIL-17RD may provide a new strategy for the therapy of RA.
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http://dx.doi.org/10.1038/s41423-020-00548-wDOI Listing
September 2020

Short Beam Shear Behavior and Failure Characterization of Hybrid 3D Braided Composites Structure with X-ray Micro-Computed Tomography.

Polymers (Basel) 2020 Aug 26;12(9). Epub 2020 Aug 26.

Innovation Platform of Intelligent and Energy-Saving Textiles, School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China.

Three-dimensional braided composite has a unique spatial network structure that exhibits the characteristics of high delamination resistance, damage tolerance, and shear strength. Considering the characteristics of braided structures, two types of high-performance materials, namely, aramid and carbon fibers, were used as reinforcements to prepare braided composites with different hybrid structures. In this study, the longitudinal and transverse shear properties of 3D braided hybrid composites were tested to investigate the influences of hybrid and structural effects. The damage characteristics of 3D braided hybrid composites under short beam shear loading underwent comprehensive morphological analysis via optical microscopy, water-logging ultrasonic scanning, and X-ray micro-computed tomography methods. It is shown that the shear toughness of hybrid braided composite has been improved at certain degrees compared with the pure carbon fiber composite under both transverse and longitudinal directions. The hybrid braided composites with aramid fiber as axial yarn and carbon fiber as braiding yarn exhibited the best shear toughness under transverse shear loading. Meanwhile, the composites with carbon fiber as axial yarn and aramid fiber as braiding yarn demonstrated the best shear toughness in the longitudinal direction. Due to the different distribution of axial and braiding yarns, the transverse shear property of hybrid braided structure excels over the longitudinal shear property. The failure modes of the hybrid braided composite under the two loading directions are considerably different. Under transverse loading, the primary failure mode of the composites is yarn fracture. Under longitudinal loading, the primary failure modes are resin fracture and fiber slip. The extensive interfacial effects and the good deformation capability of the hybrid braided composites can effectively prevent the longitudinal development of internal cracks in the pattern, improving the shear properties of braided composites.
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http://dx.doi.org/10.3390/polym12091931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564245PMC
August 2020

Ultrathin Phosphate-Modulated Co Phthalocyanine/g-CN Heterojunction Photocatalysts with Single Co-N (II) Sites for Efficient O Activation.

Adv Sci (Weinh) 2020 Aug 25;7(16):2001543. Epub 2020 Jun 25.

Key Laboratory of Functional Inorganic Materials Chemistry School of Chemistry and Material Sciences Heilongjiang University Harbin Heilongjiang 150080 China.

Realization of solar-driven aerobic organic transformation under atmospheric pressure raises the great challenge for efficiently activating O by tailored photocatalysts. Guided by theoretical calculation, phosphate groups are used to induce the construction of ultrathin Co phthalocyanine/g-CN heterojunctions (CoPc/P-CN, ≈4 nm) via strengthened H-bonding interfacial connection, achieving an unprecedented 14-time photoactivity improvement for UV-vis aerobic 2,4-dichlorophenol degradation compared to bulk CN by promoted activation of O. It is validated that more O radicals are produced through the improved photoreduction of O by accelerated photoelectron transfer from CN to the ligand of CoPc and then to the abundant single Co-N (II) catalytic sites, as endowed by the matched dimension, intimate interface even at the molecular level, and high CoPc dispersion of resulted heterojunctions. Interestingly, CoPc/P-CN also exhibits outstanding photoactivities in the aerobic oxidation of aromatic alcohols. This work showcases a feasible route to realize efficient photocatalytic O activation by exploiting the potential of ultrathin metal phthalocyanine (MPc) assemblies with abundant single-atom sites. More importantly, a universal facile strategy of H-bonding-dominating construction of MPc-involved heterojunctions is successfully established.
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http://dx.doi.org/10.1002/advs.202001543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435235PMC
August 2020

Clinical efficacy of MVD combined with PSR in the treatment of primary trigeminal neuralgia.

Exp Ther Med 2020 Aug 10;20(2):1582-1588. Epub 2020 Jun 10.

Department of Neurosurgery, Shandong Provincial Hospital, Jinan, Shandong 250021, P.R. China.

Clinical efficacy of microvascular decompression (MVD) combined with percutaneous stereotactic radiofrequency rhizotomy (PSR) in the treatment of primary trigeminal neuralgia was investigated. The medical records of 141 patients with primary trigeminal neuralgia admitted to Shandong Provincial Hospital from May 2011 to June 2013 were collected. Among them, 63 patients received MVD surgery and were set as group A, while the other 78 received MVD combined with PSR and were as group B. The efficacy and complication of the two treatment methods were compared. Multivariate logistic regression was performed to analyze the risk factors for treatment efficacy. The total effective rate was 96.15% in group B, higher than that in group A (88.89%), but the difference was not statistically significant (P>0.05). The complications in group B were statistically less than that in group A (P<0.05). Risk factors for the onset of primary trigeminal neuralgia included the degree of decompression, duration of disease, degree of compression, and clinical symptoms. Patients treated with MVD combined with PSR had a better quality of life and lower 5-year recurrence rate than patients treated with MVD (both P<0.05). In conclusion, MVD combined with PSR treatment has good clinical efficacy in primary trigeminal neuralgia and low incidence of complications. The possible risk factors for the onset of primary trigeminal neuralgia include the degree of decompression, duration of disease, degree of compression, and clinical symptoms.
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http://dx.doi.org/10.3892/etm.2020.8871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7388245PMC
August 2020

Non-O1/non-O139 bacteraemia in mainland China from 2005 to 2019: clinical, epidemiological and genetic characteristics.

Epidemiol Infect 2020 07 8;148:e186. Epub 2020 Jul 8.

Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

In mainland China, the clinical, epidemiological and genetic features of non-O1/non-O139 Vibrio cholerae (NOVC) bacteraemia have been scarcely investigated. Herein, we describe a patient with NOVC bacteraemia diagnosed in our hospital and present a retrospective analysis of literature reports of 32 other cases in China, detailing the clinical epidemiology, antibiotic resistance and molecular characteristics of isolates. Most patients were male (84.8%; median age, 53 years) and had predisposing factors, such as cirrhosis, malignant tumours, blood diseases and diabetes. In addition to fever, gastroenteritis was the most frequent presenting symptom. The mortality rate during hospitalisation was 12.1%. NOVC bacteraemia cases were more common in June-August, with the majority in coastal provinces and the Yangtze River basin. Only 42.4% of cases were attributed to consumption of marine (aquatic) products. Tetracycline, third-generation cephalosporins, and fluoroquinolones were the most effective antimicrobial agents, and the highest frequencies of resistance were recorded for ampicillin/sulbactam (37.5%), amoxicillin/clavulanic acid (33.3%), ampicillin (29.2%) and sulfamethoxazole (20%). Multi-drug resistant isolates were not detected. Limited data indicate that ctxAB and tcpA genes were absent in all NOVC isolates but other putative virulence genes (hlyA, toxR, hap and rtxA) were common. Ten multilocus sequence types were identified with marked genetic heterogeneity between different isolates. As clinical manifestations of NOVC bacteraemia may vary widely, and isolates exhibit genetic diversity, clinicians and public health experts should be alerted to the possibility of infection with this pathogen because of the high prevalence of liver disease in China.
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http://dx.doi.org/10.1017/S0950268820001545DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482189PMC
July 2020

Cyberbullying Victimization and Adolescent Depression: The Mediating Role of Psychological Security and the Moderating Role of Growth Mindset.

Int J Environ Res Public Health 2020 06 18;17(12). Epub 2020 Jun 18.

Department of Psychology, University of Turin, 10124 Torino, Italy.

The objective of this study was to examine the mechanisms (the mediating role of psychological security and the moderating role of growth mindset) underlying the association between cyberbullying victimization and depression among adolescents. A sample of 755 adolescents (M = 13.35 ± 1.02; 373 boys) was recruited from two junior high schools, and the participants were asked to voluntarily complete a set of measures, including the cyberbullying victimization subscale in the Chinese version of the Cyberbullying Inventory, the Chinese version of the Security Questionnaire, the Chinese version of the Center for Epidemiologic Studies Depression Scale, and the Growth Mindset Inventory. The results indicated that: (1) cyberbullying victimization was positively associated with depression through the mediating effect of psychological security and (2) both the direct association between cyberbullying victimization and depression and the indirect association through the mediating effect of psychological security were moderated by growth mindset. Specifically, growth mindset could significantly alleviate the adverse effects of cyberbullying victimization on psychological security and on depression. These findings not only shed light on the mechanisms linking cyberbullying victimization to depression among adolescents, but also provide an empirical basis for formulating prevention and/or intervention programs aimed at reducing depression levels and the negative influences of cyberbullying victimization among adolescents.
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http://dx.doi.org/10.3390/ijerph17124368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345096PMC
June 2020

Clinical characteristics of 16 patients with COVID-19 infection outside of Wuhan, China: a retrospective, single-center study.

Ann Transl Med 2020 May;8(10):642

Department of Infection, Affiliated Hospital of Shaoxing University (The Shaoxing Municipal Hospital), Shaoxing 312000, China.

Background: Since early December 2019, the 2019 novel coronavirus (COVID-19) has emerged in Wuhan and spread rapidly in China. We aimed to describe the clinical characteristics of hospitalized patients with confirmed COVID-19 infection in Shaoxing, and provide an insight into the treatment of COVID-19 across China and elsewhere.

Methods: In this retrospective, single-center, study, we enrolled 16 patients with laboratory-confirmed COVID-19 admitted to the Affiliated Hospital of Shaoxing University between February 24 and January 25, 2020. Epidemiological, demographic, clinical, laboratory, radiological feature, and treatment data were all collected. Outcomes were followed up until March 16, 2020.

Results: Among the 16 patients with COVID-19 infection, 11 patients (68.8%) had traveled or lived in Wuhan or surrounding areas, and 2 (12.5%) patients had exposure to patients with confirmed COVID-19 infection. The average age of the patients was 44.1 (16.5) years, and there were 10 women (62.5%) and 6 men (37.5%). More than half had chronic diseases [9 (56.3%)]. The most common symptoms at onset of COVID-19 infection were fever [12 (75%)] and cough [8 (50%)]; 11 (68.8%) patients had lymphopenia, and 12 (75%) had elevated C-reactive protein. On admission, abnormalities in computed tomography (CT) or chest X-ray images were revealed among all patients, and 11 (68.8%) of 16 patients had bilateral involvement. All patients were given psychological counseling, 15 (93.8%) patients were administered with antiviral therapy, 8 (50%) received empirical antibiotic treatment, and 5 (31.3%) patients were given systematic corticosteroids. Complications included acute respiratory distress syndrome (ARDS) requiring non-invasive mechanical ventilation [1 (6.3%)], acute respiratory injury [4 (25%)], acute renal injury [1 (6.3%)], septic shock [1 (6.3%)], liver dysfunction [5 (31.3%)], electrolyte disturbance [8 (50.0%)], and hospital-acquired pneumonia [3 (18.8%)]. None of the 16 patients died of COVID-19 pneumonia.

Conclusions: Compared with the symptoms of the initial patients with COVID-19 infection in Wuhan, the symptoms of the patients from Shaoxing city were relatively mild. Currently, there is no effective drug treatment or vaccine for COVID-19, and psychological counseling cannot be ignored. Drugs and vaccines against COVID-19 infection need to be developed as soon as possible.
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http://dx.doi.org/10.21037/atm-20-3422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7290627PMC
May 2020

Radiomic prediction models for the level of Ki-67 and p53 in glioma.

J Int Med Res 2020 May;48(5):300060520914466

Department of Radiology, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Objective: To identify glioma radiomic features associated with proliferation-related Ki-67 antigen and cellular tumour antigen p53 levels, common immunohistochemical markers for differentiating benign from malignant tumours, and to generate radiomic prediction models.

Methods: Patients with glioma, who were scanned before therapy using standard brain magnetic resonance imaging (MRI) protocols on T1 and T2 weighted imaging, were included. For each patient, regions-of-interest (ROI) were drawn based on tumour and peritumoral areas (5/10/15/20 mm), and features were identified using feature calculations, and used to create and assess logistic regression models for Ki-67 and p53 levels.

Results: A total of 92 patients were included. The best area under the curve (AUC) for the Ki-67 model was 0.773 for T2 weighted imaging in solid glioma (sensitivity, 0.818; specificity, 0.833), followed by a less reliable AUC of 0.773 (sensitivity, 0.727; specificity 0.667) in 20-mm peritumoral areas. The highest AUC for the p53 model was 0.709 (sensitivity, 1; specificity, 0.4) for T2 weighted imaging in 10-mm peritumoral areas.

Conclusion: Using T2-weighted imaging, the prediction model for Ki-67 level in solid glioma tissue was better than the p53 model. The 20-mm and 10-mm peritumoral areas in the Ki-67 and p53 model, respectively, showed predictive effects, suggesting value in further research into areas without conventional MRI features.
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http://dx.doi.org/10.1177/0300060520914466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241212PMC
May 2020

A dual-mode PCT electrochemical immunosensor with CuCoS bimetallic sulfides as enhancer.

Biosens Bioelectron 2020 Sep 12;163:112280. Epub 2020 May 12.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.

A method for the detection of procalcitonin (PCT) with improved accuracy is urgently needed. A dual-mode electrochemical immunosensor with CuCoS-Au bimetallic sulfides as enhancer for accurate detection of PCT was fabricated in this work. The immunosensor not only displays a heavy square wave voltammetry (SWV) signal due to the electron transfer of Cu and Cu but not the addition of electron mediators. But also presents high electrocatalytic activity towards HO redox reactions and enhances the detection sensitivity of the amperometric i-t curve (i-t). The introduction of Au nanoparticles, not only improves the conductivity but also has synergistic effects between Au NPs and CuCoS, which promote the detection sensitivity by SWV and i-t. To firmly immobilize antibodies, electrolytic gold was used as the matrix for the immunosensor. Based on a CuCoS-Au dual-signal indicator and electrolytic gold as the matrix, the developed immunosensor for PCT detection in this study demonstrates a wide linear response (0.0001-50 ng/mL) and a low detection limit (SWV: 82.6 fg/mL and i-t: 95.4 fg/mL). Additionally, the fabricated immunosensor exhibited an outstanding analytical approach for PCT and broad application for other biomarkers in clinical diagnosis and treatment.
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http://dx.doi.org/10.1016/j.bios.2020.112280DOI Listing
September 2020

Association between diabetic retinopathy and interleukin-related gene polymorphisms: a machine learning aided meta-analysis.

Ophthalmic Genet 2020 06 13;41(3):216-222. Epub 2020 Apr 13.

R&D Department, Nanjing Regenerative Medicine Engineering and Technology Research Center , Nanjing, Jiangsu, China.

Background: Diabetic retinopathy (DR) is a severe complication of diabetes and a common cause of visual loss in adults. We aimed to assess the correlation between IL gene-related SNPs and the incidence of DR and attempted to predict DR with combined mutation site detection.

Methods: A systematic search of databases was performed up to August 2019. Five genetic models were used to analyze associations. Machine learning methods were implemented to improve SNP-related disease prediction.

Results: Sixteen trials assessing a total of 7221 patients were included in our meta-analysis. IL6/rs1800795, rs1800796, and IL10/rs1800896 were analyzed. For the IL-6 gene, there was no significant association between rs1800795 and the incidence of DR (allelic model: OR, 1.091; 95% CI, 0.892-1.334; = .396). There was no significant correlation between rs1800796 (allelic model: OR, 1.135; 95% CI, 0.678-1.901; = .63), rs1800896 (allelic model: OR, 1.047; 95% CI, 0.788-1.392; = .752) and the incidence of DR. Unfortunately, the machine learning results also showed that the combined detection of two SNPs could not accurately predict DR occurrence.

Conclusion: rs1800795 and rs1800796 in the IL-6 gene and rs1800896 in IL-10 gene are not related to the incidence of DR. Mutations in multiple SNPs for each DR patient still need to be specifically assessed to increase prediction accuracy.
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http://dx.doi.org/10.1080/13816810.2020.1747091DOI Listing
June 2020

Separating and recycling metal mixture of pyrolyzed waste printed circuit boards by a combined method.

Waste Manag 2020 Apr 8;107:113-120. Epub 2020 Apr 8.

Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, China.

Waste printed circuit boards (WPCBs) contain a variety of valuable and hazardous materials. Recycling WPCBs is an important subject not only for environmental protection but also for sustainable development of resources. In this work, a new method combined low-temperature alkaline smelting with liquid-liquid phase separation is proposed to separate and recycle metal mixture in pyrolysis residue of WPCBs of mobile phones. During the low-temperature alkaline smelting process, amphoteric metals Al, Pb, Si, Sn, and Zn are firstly separated and recycled from the metal mixture with the separation rates of 99.5%, 81.6%, 97.8%, 88.4% and 95.7%, respectively. To separate the remaining metal mixture mainly containing elements Cu, Fe, Cr, Ni, Au and Ag, a liquid-liquid phase separation system is designed. As a result, the noble metals Au and Ag are concentrated in the copper-rich substance to form a high-value group, while the elements Ni and Cr distribute in the iron-rich substance. The iron-rich substance can be reused in the liquid-liquid phase separation process. In the super-gravity field, the recycling rates of the metals Au, Ag, Cr and Ni reach 98.1%, 99.8%, 95.6% and 75.4%, respectively. Furthermore, the iron-rich substance can be reused back to the liquid-liquid separation system. The copper-rich substance enriched by the noble metals can be efficiently recovered with low energy consumption and less pollution. This work provides an environmentally friendly and efficient route for separating and recycling the metal mixture in WPCBs.
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http://dx.doi.org/10.1016/j.wasman.2020.04.006DOI Listing
April 2020

Why Do People With Self-Control Forgive Others Easily? The Role of Rumination and Anger.

Front Psychol 2020 20;11:129. Epub 2020 Feb 20.

Key Laboratory of Adolescent Cyberpsychology and Behavior, Ministry of Education, Wuhan, China.

Previous research shows that self-control predicts forgiveness, but few studies have investigated the internal mechanism of this link. The current study explored the effects of rumination and anger on the relationship between self-control and forgiveness. A total of 580 college students recruited from three universities in Wuhan completed the self-control, rumination, anger, and trait forgiveness scales. Results showed that self-control was positively correlated with forgiveness ( = 0.34, < 0.001). Rumination ( = 0.08, < 0.05) and anger ( = 0.13, < 0.05) mediate the relationship between self-control and forgiveness. Moreover, a serial mediation effect of rumination and anger was observed between self-control and trait forgiveness ( = 0.02, < 0.05). These findings suggest that self-control may impair individuals' rumination. Moreover, less rumination may restrain anger and consequently increase forgiveness.
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http://dx.doi.org/10.3389/fpsyg.2020.00129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7045615PMC
February 2020

Heparin coated meta-organic framework co-delivering doxorubicin and quercetin for effective chemotherapy of lung carcinoma.

J Int Med Res 2020 Feb;48(2):300060519897185

Department of Respiratory Medicine, Affiliated Hospital of Shaoxing University of Arts and Sciences, Shaoxing, Zhejiang Province, China.

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http://dx.doi.org/10.1177/0300060519897185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111025PMC
February 2020

Triple Amplification of 3,4,9,10-Perylenetetracarboxylic Acid by Co-Based Metal-Organic Frameworks and Silver-Cysteine and Its Potential Application for Ultrasensitive Assay of Procalcitonin.

ACS Appl Mater Interfaces 2020 Feb 12;12(8):9098-9106. Epub 2020 Feb 12.

Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering , University of Jinan , Jinan 250022 , P. R. China.

In this work, a triple-amplified biosensor with a bioactivity-maintained peculiarity was constructed for quantitative procalcitonin (PCT) detection. As everyone knows, a strong electrochemiluminescence (ECL) signal is the premise to ensure high sensitivity for trace target detection. Hence, a valid tactic was developed to achieve signal amplification of luminophor by using Co-based metal-organic frameworks (ZIF-67) and silver-cysteine (AgCys). The ZIF-67 particles, which have more atomically dispersed Co, could play the role of a co-reaction accelerator to catalyze SO to generate abundant Co and sulfate radical anions (SO). Afterward, a mass of Co was reduced to more hydroxyl radicals (OH) by HO, thus ulteriorly reducing SO to generate more SO. Remarkably, SO was reduced to SO continuously with the recycling of Co and Co, which realized an effective signal amplification. Meanwhile, the AgCys complex with superior catalysis and biocompatibility was prepared to further improve the ECL signal and maintain the bioactivity of the biomolecule. Furthermore, HWRGWVC, a heptapeptide that was used for combining the Fc fragments of an antibody by Au-S bonding to achieve the fixed point fixation, could not only maintain bioactivity of an antibody but also improved its incubation efficiency, thus further enhancing biosensor sensitivity. Under optimum conditions, the proposed biosensor realized highly sensitive assay for PCT with a wide dynamic range from 10 fg/mL to 100 ng/mL and a detection limit as low as 3.67 fg/mL. With superior stability, selectivity, and repeatability, the prepared biosensor revealed immense potential application of ultrasensitive assay for PCT in human serum.
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http://dx.doi.org/10.1021/acsami.9b23248DOI Listing
February 2020

Deep Multilayer Brain Proteomics Identifies Molecular Networks in Alzheimer's Disease Progression.

Neuron 2020 03 8;105(6):975-991.e7. Epub 2020 Jan 8.

Departments of Psychiatry and Neuroscience, The Alzheimer's Disease Research Center, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Mental Illness Research, Education and Clinical Center (MIRECC), James J. Peters VA Medical Center, Bronx, NY 10468, USA.

Alzheimer's disease (AD) displays a long asymptomatic stage before dementia. We characterize AD stage-associated molecular networks by profiling 14,513 proteins and 34,173 phosphosites in the human brain with mass spectrometry, highlighting 173 protein changes in 17 pathways. The altered proteins are validated in two independent cohorts, showing partial RNA dependency. Comparisons of brain tissue and cerebrospinal fluid proteomes reveal biomarker candidates. Combining with 5xFAD mouse analysis, we determine 15 Aβ-correlated proteins (e.g., MDK, NTN1, SMOC1, SLIT2, and HTRA1). 5xFAD shows a proteomic signature similar to symptomatic AD but exhibits activation of autophagy and interferon response and lacks human-specific deleterious events, such as downregulation of neurotrophic factors and synaptic proteins. Multi-omics integration prioritizes AD-related molecules and pathways, including amyloid cascade, inflammation, complement, WNT signaling, TGF-β and BMP signaling, lipid metabolism, iron homeostasis, and membrane transport. Some Aβ-correlated proteins are colocalized with amyloid plaques. Thus, the multilayer omics approach identifies protein networks during AD progression.
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http://dx.doi.org/10.1016/j.neuron.2019.12.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318843PMC
March 2020

Mixed-Metal-Cluster Strategy for Boosting Electrocatalytic Oxygen Evolution Reaction of Robust Metal-Organic Frameworks.

ACS Appl Mater Interfaces 2019 Dec 21;11(48):45080-45086. Epub 2019 Nov 21.

School of Materials Science and Engineering, College of Chemical and Environmental Engineering , Harbin University of Science and Technology , Harbin 150040 , China.

Rational construction of mixed metal-organic frameworks (MOFs) has been proved to be an effective way to heighten the electrocatalytic performance for MOFs, while the function of mixed metal clusters in MOFs to the electrocatalytic activity has not been known. For the first time, we present a mixed-metal-cluster strategy to boost electrocatalytic oxygen evolution reaction (OER) performance for MOFs. Heterometal clusters (FeM(μ-O) (CHCOO)(HO), denoted as FeM (M: Co or Ni)), were chosen as the metal source to construct two bimetal PCN-250-FeM. Then, we further mixed FeCo and FeNi clusters to construct mixed-metal-cluster PCN-FeCo-FeNi by the in situ solvothermal reaction. Consequently, the OER activity of PCN-FeCo-FeNi shows a dramatic enhancement compared with that of the parent bimetal MOFs. The PCN-FeCo-FeNi displays a lower overpotential of 271 mV (η), small Tafel slope (67.7 mV dec), and good linear sweep voltammetry cycle stability for the OER. Combination of DFT calculation and experiment results show that the improved electrocatalytic activity of PCN-FeCo-FeNi is ascribed to the increased electron density of Co and Ni active centers and electrochemically active areas.
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http://dx.doi.org/10.1021/acsami.9b14995DOI Listing
December 2019

Are multi-contrast magnetic resonance images necessary for segmenting multiple sclerosis brains? A large cohort study based on deep learning.

Magn Reson Imaging 2020 01 25;65:8-14. Epub 2019 Oct 25.

Department of Diagnostic and Interventional Imaging, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, United States of America.

Background: Magnetic resonance images with multiple contrasts or sequences are commonly used for segmenting brain tissues, including lesions, in multiple sclerosis (MS). However, acquisition of images with multiple contrasts increases the scan time and complexity of the analysis, possibly introducing factors that could compromise segmentation quality.

Objective: To investigate the effect of various combinations of multi-contrast images as input on the segmented volumes of gray (GM) and white matter (WM), cerebrospinal fluid (CSF), and lesions using a deep neural network.

Methods: U-net, a fully convolutional neural network was used to automatically segment GM, WM, CSF, and lesions in 1000 MS patients. The input to the network consisted of 15 combinations of FLAIR, T1-, T2-, and proton density-weighted images. The Dice similarity coefficient (DSC) was evaluated to assess the segmentation performance. For lesions, true positive rate (TPR) and false positive rate (FPR) were also evaluated. In addition, the effect of lesion size on lesion segmentation was investigated.

Results: Highest DSC was observed for all the tissue volumes, including lesions, when the input was combination of all four image contrasts. All other input combinations that included FLAIR also provided high DSC for all tissue classes. However, the quality of lesion segmentation showed strong dependence on the input images. The DSC and TPR values for inputs with the four contrast combination and FLAIR alone were very similar, but FLAIR showed a moderately higher FPR for lesion size <100 μl. For lesions smaller than 20 μl all image combinations resulted in poor performance. The segmentation quality improved with lesion size.

Conclusions: Best performance for segmented tissue volumes was obtained with all four image contrasts as the input, and comparable performance was attainable with FLAIR only as the input, albeit with a moderate increase in FPR for small lesions. This implies that acquisition of only FLAIR images provides satisfactory tissue segmentation. Lesion segmentation was poor for very small lesions and improved rapidly with lesion size.
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http://dx.doi.org/10.1016/j.mri.2019.10.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918476PMC
January 2020

Brain and lesion segmentation in multiple sclerosis using fully convolutional neural networks: A large-scale study.

Mult Scler 2020 09 13;26(10):1217-1226. Epub 2019 Jun 13.

Department of Diagnostic and Interventional Imaging, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA.

Objective: To investigate the performance of deep learning (DL) based on fully convolutional neural network (FCNN) in segmenting brain tissues in a large cohort of multiple sclerosis (MS) patients.

Methods: We developed a FCNN model to segment brain tissues, including T2-hyperintense MS lesions. The training, validation, and testing of FCNN were based on ~1000 magnetic resonance imaging (MRI) datasets acquired on relapsing-remitting MS patients, as a part of a phase 3 randomized clinical trial. Multimodal MRI data (dual-echo, FLAIR, and T1-weighted images) served as input to the network. Expert validated segmentation was used as the target for training the FCNN. We cross-validated our results using the leave-one-center-out approach.

Results: We observed a high average (95% confidence limits) Dice similarity coefficient for all the segmented tissues: 0.95 (0.92-0.98) for white matter, 0.96 (0.93-0.98) for gray matter, 0.99 (0.98-0.99) for cerebrospinal fluid, and 0.82 (0.63-1.0) for T2 lesions. High correlations between the DL segmented tissue volumes and ground truth were observed ( > 0.92 for all tissues). The cross validation showed consistent results across the centers for all tissues.

Conclusion: The results from this large-scale study suggest that deep FCNN can automatically segment MS brain tissues, including lesions, with high accuracy.
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http://dx.doi.org/10.1177/1352458519856843DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908772PMC
September 2020

The Prognostic and Predictive Value of Dihydropyrimidine Dehydrogenase-Related Indicators in Clinical Outcomes of Chemotherapy in Colorectal Cancer Patients: a Systematic Review and Meta-Analysis.

Pathol Oncol Res 2020 Jan 5;26(1):121-131. Epub 2018 Dec 5.

R&D department, Nanjing Regenerative Medicine Engineering and Technology Research Center, No.108, Ganjiabian East, Qixia District, Nanjing, 210046, Jiangsu, China.

Colorectal cancer (CRC) is the third most common cancer worldwide. Predictive biomarkers are needed to predict patients' outcomes and to select a chemotherapy regimen. We assessed whether dihydropyrimidine dehydrogenase (DPD)-related indicators can predict CRC patients' outcomes. We searched the studies in PubMed, EmBase, and the Cochrane Library up to March 4, 2018. We mainly analyzed different CRC patients' outcomes according to specific DPD-related indicators. Twenty-five articles were included in the meta-analysis. The results showed that for disease-free survival (DFS), low DPD expression was significantly superior to high expression (I = 72%; HR: 1.59; 95%CI: 1.21-2.09; p = 0.001). However, this result had a potential publication bias (Begg's test: p = 0.007; Egger's test: p = 0.004). Among patients treated with chemotherapy, a high thymidylate phosphorylase (TP)/DPD ratio was advantageous for DFS (I = 63.7%; HR: 0.65; 95%CI: 0.46-0.92; p = 0.015), and this result did not have a publication bias. For overall survival (OS), low DPD expression was superior to high expression (I = 74.4%; HR: 2.11; 95%CI: 1.48-3.00; p < 0.001), although this result had a publication bias (Egger's test: p = 0.003; Begg's test: p = 0.010). There was no difference in OS according to the TP/DPD ratio (I = 0%; HR: 0.92; 95%CI: 0.75-1.13; p = 0.420). DFS and OS were better in CRC patients with low DPD expression than in those with high DPD expression. However, because of publication bias, more DPD indicator-related studies, especially with negative results, are still needed. Patients with a high TP/DPD ratio have better DFS but not OS.
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http://dx.doi.org/10.1007/s12253-018-00563-3DOI Listing
January 2020