Publications by authors named "Xiaojun Ma"

296 Publications

Development and Application of 3D Bioprinted Scaffolds Supporting Induced Pluripotent Stem Cells.

Biomed Res Int 2021 13;2021:4910816. Epub 2021 Sep 13.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Three-dimensional (3D) bioprinting is a revolutionary technology that replicates 3D functional living tissue scaffolds in vitro by controlling the layer-by-layer deposition of biomaterials and enables highly precise positioning of cells. With the development of this technology, more advanced research on the mechanisms of tissue morphogenesis, clinical drug screening, and organ regeneration may be pursued. Because of their self-renewal characteristics and multidirectional differentiation potential, induced pluripotent stem cells (iPSCs) have outstanding advantages in stem cell research and applications. In this review, we discuss the advantages of different bioinks containing human iPSCs that are fabricated by using 3D bioprinting. In particular, we focus on the ability of these bioinks to support iPSCs and promote their proliferation and differentiation. In addition, we summarize the applications of 3D bioprinting with iPSC-containing bioinks and put forward new views on the current research status.
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http://dx.doi.org/10.1155/2021/4910816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452409PMC
September 2021

SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators.

Nucleic Acids Res 2021 Sep;49(17):9633-9647

Department of Biomedical Informatics, University of Pittsburgh, Pittsburgh, PA 15206, USA.

The identity and functions of specialized cell types are dependent on the complex interplay between signaling and transcriptional networks. Recently single-cell technologies have been developed that enable simultaneous quantitative analysis of cell-surface receptor expression with transcriptional states. To date, these datasets have not been used to systematically develop cell-context-specific maps of the interface between signaling and transcriptional regulators orchestrating cellular identity and function. We present SPaRTAN (Single-cell Proteomic and RNA based Transcription factor Activity Network), a computational method to link cell-surface receptors to transcription factors (TFs) by exploiting cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) datasets with cis-regulatory information. SPaRTAN is applied to immune cell types in the blood to predict the coupling of signaling receptors with cell context-specific TFs. Selected predictions are validated by prior knowledge and flow cytometry analyses. SPaRTAN is then used to predict the signaling coupled TF states of tumor infiltrating CD8+ T cells in malignant peritoneal and pleural mesotheliomas. SPaRTAN enhances the utility of CITE-seq datasets to uncover TF and cell-surface receptor relationships in diverse cellular states.
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http://dx.doi.org/10.1093/nar/gkab745DOI Listing
September 2021

Bioconversion of renewable lignocellulosic biomass into multicomponent substrate via pressurized hot water pretreatment for bioplastic polyhydroxyalkanoate accumulation.

Bioresour Technol 2021 Nov 24;339:125667. Epub 2021 Jul 24.

Ministry of Agriculture Key Laboratory of Biology and Genetic Resource Utilization of Rubber Tree/State Key Laboratory Breeding Base of Cultivation & Physiology for Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China.

The pretreatment of lignocellulosic biomass (LB) has become an important process to reduce the cost of polyhydroxyalkanoate (PHA) production. In this study, an economical and effective pressurized hot water pretreatment was used to investigate on bioconversion of four typical LB (rubber wood, sugarcane bagasse, sorghum stalk, cassava stalk) into reducing sugar, then as feedstock to accumulate PHA by mixed microbial cultures. The results showed that pretreatment changed the surface morphology of LB and further improved saccharification efficiency. The maximum reducing sugar yield of 620.7 mg·g (438.7 mg·g glucose and 178.0 mg·g xylose) was obtained by rubber wood. The conversion of cellulose and hemicellulose reached 83.10% and 78.22%. Moreover, PHA content reached the maximum of 773.2 mg COD·L in the operation cycle of 24 h. The results demonstrated that hot water pretreatment was an effective physical process to improve the saccharification efficiency of LB for reducing the cost of PHA.
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http://dx.doi.org/10.1016/j.biortech.2021.125667DOI Listing
November 2021

The empirical decomposition and peak path of China's tourism carbon emissions.

Environ Sci Pollut Res Int 2021 Jul 31. Epub 2021 Jul 31.

School of Statistics, Dongbei University of Finance and Economics, Dalian, 116025, China.

Carbon emissions from tourism are an important indicator to measure the impact of tourism on environmental quality. As the world's largest industry, tourism has many related industries and is a strong driver of energy consumption. The emission reductions it can achieve will directly determine whether China's overall carbon emission reduction target can be met. This paper analyzes the drivers of the evolution of carbon emissions from the tourism industry in China over the period 2000-2017 as a research sample using the Generalized Dividing Index Method (GDIM), and on this basis, it uses scenario analysis and Monte Carlo simulation to predict the carbon peak in tourism for the first time. The research results show that the scale of industry and energy consumption are the key factors leading to increased tourism carbon emissions, and the carbon intensity of tourism industry, energy consumption carbon intensity, investment efficiency, and energy intensity are the main factors leading to reduced carbon emissions from tourism. The scale of investment and the carbon intensity of investment have a dual effect; the scenario analysis and Monte Carlo simulation used to predict peak carbon in China's tourism industry show that the peak carbon will occur approximately in 2030. The government needs to further guide and encourage the tourism industry to increase investment activities targeting energy conservation and emission reduction. Under the conditions of strictly implementing energy conservation and emission reduction measures and vigorous promotion of the transformation and upgrading of tourism development methods, the tourism industry will have considerable potential to reduce carbon emissions.
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http://dx.doi.org/10.1007/s11356-021-14956-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8325416PMC
July 2021

Insights into enhanced polyhydroxyalkanoate production by the synergistic use of waste wood hydrolysate and volatile fatty acids by mixed microbial cultures.

Bioresour Technol 2021 Oct 2;337:125488. Epub 2021 Jul 2.

College of Light Industry Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, PR China.

The feasibility of producing polyhydroxyalkanoate (PHA) from pretreated waste wood hydrolysate and volatile fatty acids (VFAs) from sewage fermented products as co-substrate feedstock through mixed microbial cultures (MMCs) process was explored. The results showed that the addition of co-substrate shortened the cycle of PHA reaching the maximum and increased the proportion of 3-hydroxyvalerate (3HV) monomer. Compared with N-excess supply, almost 1.6 times increased PHA accumulation was realized under N-limitation, and simultaneously the highest proportion of 3HV monomer with 51% was also obtained. Additionally, PHA production in S1400 reactor reached a maximum value of about 3088 mg COD/L with culture time to 36 h. The microbial community also displayed a high diversity, which was composed of 65 bacterial genera. It is a novel attempt to accumulate PHA from pretreated waste wood hydrolysate and VFAs co-substrate through MMCs, providing an effective green approach to reduce its expensive cost and achieve mass production.
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http://dx.doi.org/10.1016/j.biortech.2021.125488DOI Listing
October 2021

Albiflorin alleviates cognitive dysfunction in STZ-induced rats.

Aging (Albany NY) 2021 07 28;13(14):18287-18297. Epub 2021 Jul 28.

Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.

Background: To explore the effect of albiflorin (AL) on streptozotocin (STZ)-induced Alzheimer's disease (AD) in rats.

Methods: A mouse model of diabetic encephalopathy was established by intraperitoneal injection of 1%STZ. Step down test and water maze test were used to test the cognitive function of rats. Congo Red Staining was used to detect the distribution of Aβ plaques in the hippocampus of rats. Cytokine levels in serum and hippocampus were measured using ELISA. Serum insulin, oral glucose tolerance (OGTT), serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured by commercial kits. And the content of Nrf-2/HO-1/HMGB1/NF-kB in the hippocampus of diabetic rats were detected by western blot.

Results And Conclusion: Compared with the STZ model group, the average escape latency of rats in the AL group in the Morris water maze test was significantly shortened, and the average number of platform crossings and the ratio of distance/total swimming distance in the target quadrant were increased significantly. Staining of tissue sections and ELISA showed a decrease in Aβ plaque density in the hippocampus of rats in the AL group. And serum insulin levels of rats in the ALgroup were significantly reduced and OGTT was improved. In addition, AL could also regulate the Nrf-2/HO-1/HMGB1/NF-kB signal pathway in the hippocampus. Therefore, AL may ameliorate STZ-induced cognitive impairment in rats by regulating oxidative stress and inflammation in the brain.
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http://dx.doi.org/10.18632/aging.203274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351685PMC
July 2021

Do reverse total shoulder replacements have better clinical and functional outcomes than hemiarthroplasty for patients undergoing proximal humeral tumor resection using devitalized autograft composite reconstruction: a case-control study.

J Orthop Surg Res 2021 Jul 14;16(1):453. Epub 2021 Jul 14.

Department of Orthopedic Oncology, Shanghai General Hospital Affiliated with Shanghai Jiaotong University, No. 100 Haining Road, Hongkou District, Shanghai, 200080, China.

Objective: To compare the efficacy and prognosis of reverse total shoulder arthroplasty (rTSA) with shoulder hemiarthroplasty (SHA) using devitalized autograft or allograft composite reconstruction after proximal humeral tumor resection.

Methods: We retrospectively reviewed patients who underwent SHA (32) and rTSA (20) for tumor resections of the proximal humerus from January 2014 to July 2020. The clinical results included duration of the operation, intraoperative blood loss, bone union, visual analog scale (VAS) score, shoulder range of motion (ROM), American Shoulder and Elbow Surgeons (ASES) shoulder score, recurrence, and overall survival.

Results: Fifty-two patients were followed up for a mean of 30 months. Thirty-two patients were SHA with allograft-prosthetic composite (APC) reconstructions, while other 20 were rTSA with devitalized autograft-prosthetic composite reconstructions. At the end of the follow-up, 2 recurrence, 3 postoperative infections, and 4 subluxations occurred among the SHA patients. Two patients in the rTSA group had postoperative anterior dislocation and underwent revision surgery with surgical mesh, and 2 (2/20) had grade II scapular notching. The mean VAS score of the shoulder was 1.5 ± 0.8 in the rTSA group and 2.3 ± 1.2 in the SHA group (p < 0.05). The mean active forward flexion of the shoulder joint was 50.6 ± 6.0 in the SHA group and 100 ± 7.6 in the rTSA group (p < 0.05). The ASES shoulder score was 78 ± 3.0 in the rTSA group and 52 ± 5.6 in the SHA group (p < 0.05). The overall 3-year survival rate of all patients was 60.0%, and patients in the rTSA group showed better survival in terms of the mean 3-year OS than patients in the SHA group (p = 0.04).

Conclusion: rTSA with devitalized autograft-prosthetic composite can offer a reasonable reconstruction of the shoulder joint after Malawer type I tumor resection. Compared with patients who underwent SHA, patients who underwent rTSA present good outcomes, a better range of motion, better bone union, and no increase in instability rate in the mid-term.
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http://dx.doi.org/10.1186/s13018-021-02488-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278672PMC
July 2021

Surgical Treatment of Sacral Metastatic Tumors.

Front Oncol 2021 25;11:640933. Epub 2021 Jun 25.

Department of Orthopedics, Shanghai General Hospital, Shanghai, China.

Objective: This study intends to retrospectively analyze the data of patients with sacral metastases in our center, and analyze the treatment methods and therapeutic effects of sacral metastases.

Methods: 73 patients with sacral metastases treated in our hospital from June 2013 to June 2019 were retrospectively analyzed. There were 54 cases of neurological symptoms, 42 cases of sacroiliac joint instability, 24 cases of lower limb muscle weakness and 19 cases of abnormal urination and defecation. Four patients with tumors below S3 underwent complete tumor resection, 23 patients with tumors above S3 and without sacroiliac joint instability underwent tumor curettage and nerve root lysis, 34 patients with tumors above S3 and sacroiliac joint instability underwent tumor curettage, nerve root release and screw rod reconstruction. 12 patients with multiple metastases underwent percutaneous radiofrequency ablation and sacroplasty. VAS was used to evaluate the preoperative and postoperative pain scores, and the postoperative pain relief, neurological function, bowel function, wound healing and complications were evaluated.

Results: There were no perioperative death, 8 cases of poor wound healing, 5 cases of nerve injury, postoperative sensory and motor loss of lower limbs. Cerebrospinal fluid (CSF) leak in 7 cases. The patients were followed up for 6-25 months (mean 12 months). The VAS scores of patients with pain symptoms were 7 points before operation and 1.44 points after operation, In 19 patients with abnormal urination and defecation function, 12 patients recovered to normal 3-6 months after operation, 5 cases had no significant change compared with preoperative, and 2 cases had aggravated symptoms; 17 cases of patients with lower limb muscle strength were significantly recovered after operation, and the average muscle strength was increased by 2 grades; 30 cases of patients with unstable sacroiliac joint got internal fixation had significantly pain relief. Pain symptoms of 9 patients were significantly relieved after percutaneous radiofrequency ablation.

Conclusion: the operation of sacral metastases mainly adopts a relatively conservative surgical method, which can effectively improve the quality of life of patients with sacral metastases by retaining the nerve function and relieving the pain of patients, combining with radiofrequency ablation, sacroplasty and targeted drugs.
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http://dx.doi.org/10.3389/fonc.2021.640933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267802PMC
June 2021

Bromodomain Inhibition Attenuates the Progression and Sensitizes the Chemosensitivity of Osteosarcoma by Repressing GP130/STAT3 Signaling.

Front Oncol 2021 8;11:642134. Epub 2021 Jun 8.

Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Osteosarcoma is the most common primary malignant bone tumor, and there are few ideal clinically available drugs. The bromodomain and extraterminal domain (BET) protein is an emerging target for aggressive cancer, but therapies targeting the BET in osteosarcoma have been unsuccessful in clinical trials to date, and further exploration of specific BET inhibitors is of great significance. In our study, we demonstrated that NHWD-870, a potent BET inhibitor in a phase I clinical trial, significantly inhibited tumor proliferation and promoted cell apoptosis by reversing the oncogenic signature in osteosarcoma. More importantly, we identified NHWD-870 impeded binding of BRD4 to the promoter of GP130 leading to diminished activation of JAK/STAT3 signaling pathway. Furthermore, GP130 knockdown significantly sensitizes the chemosensitivity . In OS cell-derived xenografts, NHWD-870 effectively inhibited the growth of osteosarcoma. Beyond that, NHWD-870 effectively inhibited the differentiation and maturation of precursor osteoclasts and attenuated osteoclast-mediated bone loss . Finally, we confirmed the efficacy of synthetic lethal effects of NHWD-870 and cisplatin in antagonizing osteosarcoma in a preclinical PDX model. Taken together, these findings demonstrate that NHWD-870, as an effective BET inhibitor, may be a potential candidate for osteosarcoma intervention linked to its STAT3 signaling inhibitory activity. In addition, NHWD-870 appears to be a promising therapeutic strategy for bone-associated tumors, as it interferes with the vicious cycle of tumor progression and bone destruction.
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http://dx.doi.org/10.3389/fonc.2021.642134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219214PMC
June 2021

Discovery of novel N-sulfonamide-tetrahydroisoquinolines as potent retinoic acid receptor-related orphan receptor γt agonists.

Eur J Med Chem 2021 Oct 1;222:113585. Epub 2021 Jun 1.

Department of Medicinal Chemistry, School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai, 201203, China. Electronic address:

Cancer immunotherapy has become a research hotspot in recent years. A variety of targets were developed for small molecule immuno-oncology agents, including retinoic acid-related orphan receptor gamma t (RORγt), chemokine receptor, stimulator of interferon genes (Sting), indoleamine 2,3-dioxygenase (IDO), toll-like receptors (TLR), etc. Among them, the retinoic acid receptor-related orphan receptor γt (RORγt) has gradually attracted more attention in these years. In particular, LYC-55716 (cintirorgon), a small molecule RORγt agonist developed by Lycera, has entered the phase II clinical study. In this work, starting from compound 7, compound 28 was obtained after 4 rounds of compound design, synthesis and SAR studies, which had an EC of 0.021 ± 0.002 μM in dual Fluorescence Resonance Energy Transfer (dual-FRET) assay and an EC of 0.021 ± 0.002 μM in mouse Th17 cell differentiation assay. It indicated that compound 28 had excellent RORγt agonistic activity and was expected to be developed as a new type of small molecule drug for cancer immunotherapy. The molecular dynamic simulation revealed that the agonist 28 formed a strong HYF triplet intramolecular interaction to stabilize H12, which helped RORγt to form the protein-binding site and therefore made the receptor ready to recruit coactivator. When the inverse agonist s27 bound with RORγt, the steric hindrance between s27 and H479 caused the destruction of the HYF triplet, leading to the collapse of H12, thus the transcription function of RORγt was interrupted due to the failure of recruiting a coactivator molecule. The triplet HYF in RORγt and the rigidity of 28 and s27 were identified to be the structural determinants for the functional switch of RORγt.
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http://dx.doi.org/10.1016/j.ejmech.2021.113585DOI Listing
October 2021

Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases.

Phytochemistry 2021 Sep 6;189:112824. Epub 2021 Jun 6.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China. Electronic address:

Siraitia grosvenorii (Luo hanguo or monk fruit) is a valuable medicinal herb for which the market demand has increased dramatically worldwide. As promising natural sweeteners, mogrosides have received much attention from researchers because of their extremely high sweetness and lack of calories. Nevertheless, owing to the absence of genetic transformation methods, the molecular mechanisms underlying the regulation of mogroside biosynthesis have not yet been fully elucidated. Therefore, an effective method for gene function analysis needs to be developed for S. grosvenorii fruit. As a powerful approach, transient expression has become a versatile method to elucidate the biological functions of genes and proteins in various plant species. In this study, PBI121 with a β-glucuronidase (GUS) marker and tobacco rattle virus (TRV) were used as vectors for overexpression and silencing, respectively, of the SgCPR1 and SgCPR2 genes in S. grosvenorii fruit. The effectiveness of transient expression was validated by GUS staining in S. grosvenorii fruit, and the expression levels of SgCPR1 and SgCPR2 increased significantly after infiltration for 36 h. In addition, TRV-induced gene silencing suppressed the expression of SgCPR1 and SgCPR2 in S. grosvenorii fruit. More importantly, the production of the major secondary metabolites mogrol, mogroside IIE (MIIE) and mogroside III (MIII) was activated by the overexpression of SgCPR1 and SgCPR2 in S. grosvenorii fruit, with levels 1-2 times those in the control group. Moreover, the accumulation of mogrol, MIIE and MIII was decreased in the SgCPR1 and SgCPR2 gene silencing assays. Therefore, this transient expression approach was available for S. grosvenorii fruit, providing insight into the expression of the SgCPR1 and SgCPR2 genes involved in the mogroside biosynthesis pathway. Our study also suggests that this method has potential applications in the exploration of the molecular mechanisms, biochemical hypotheses and functional characteristics of S. grosvenorii genes.
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http://dx.doi.org/10.1016/j.phytochem.2021.112824DOI Listing
September 2021

LncRNA TUG1 ameliorates diabetic nephropathy via inhibition of PU.1/RTN1 signaling pathway.

J Leukoc Biol 2021 Jun 1. Epub 2021 Jun 1.

Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Diabetic nephropathy (DN) is a leading cause of end-stage renal failure. The study aimed to investigate whether long noncoding RNA taurine-upregulated gene 1 (TUG1) can ameliorate the endoplasmic reticulum stress (ERS) and apoptosis of renal tubular epithelial cells in DN, and the underlying mechanism. The DN mouse model was established by streptozocin injection, and the human renal tubular epithelial cell line HK-2 was treated with high glucose (HG) to mimic DN in vitro. The molecular mechanism was explored through dual-luciferase activity assay, RNA pull-down assay, RNA immunoprecipitation (RIP), and chromatin immunoprecipitation (CHIP) assay. The expression of TUG1 was significantly decreased in the renal tubules of DN model mice. Overexpression of TUG1 reduced the levels of ERS markers and apoptosis markers by inhibiting reticulon-1 (RTN1) expression in HG-induced HK-2 cells. Furthermore, TUG1 down-regulated RTN1 expression by inhibiting the binding of transcription factor PU.1 to the RTN1 promoter, thereby reducing the levels of ERS markers and apoptosis markers. Meanwhile, TUG1-overexpression adenovirus plasmids injection significantly alleviated tubular lesions, and reduced RTN1 expression, ERS markers and apoptosis markers, whereas these results were reversed by injection of PU.1-overexpression adenovirus plasmids. TUG1 restrains the ERS and apoptosis of renal tubular epithelial cells and ameliorates DN through inhibition of transcription factor PU.1.
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http://dx.doi.org/10.1002/JLB.6A1020-699RRRDOI Listing
June 2021

Indian dust-rain storm: Possible influences of dust ice nuclei on deep convective clouds.

Sci Total Environ 2021 Jul 16;779:146439. Epub 2021 Mar 16.

Innovation Center of Ocean-Atmosphere System Observation and Prediction, Zhuhai Fudan Innovation Institute, Zhuhai 519000, China.

Estimating the influence of dust aerosol on clouds, especially deep convective clouds which is closely related to heavy precipitation, still has large uncertainties due to the lack of adequate direct measurements. In this study, a typical dust storm along with thunderstorm (referred to dust-rain storm), occurred in Northwest India on May 2, 2018, was selected to explore the possible effects of dust aerosol on deep convective cloud by combining a series of satellite retrievals and reanalysis data. Results showed that dust aerosol and moisture were carried to Northwest India by southwesterly wind at 700 hPa and easterly wind along south foothill of Himalayas at 850 hPa, respectively, and then were lifted to upper level of the cloud by robust updraft induced by the deep convection and secondary circulation driven by the upper-level westerly jet. The injection of dust is likely to transfer supercooled water cloud into ice cloud as effective ice nuclei, hence increasing the cloud ice water path and cloud optical depth but decreasing ice particle radius in the cloud. The latent heat released by this phase-change process would enhance the deep convection and further cause heavy rainfall in northern India by drawing moisture from surrounding region. Although we cannot eliminate the effect of large-scale dynamics, this study highlighted the role of dust aerosol in invigorating the deep convective clouds as ice nuclei, providing observation evidence for the investigation of aerosol-cloud-precipitation interaction.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146439DOI Listing
July 2021

Hypothalamus-Pituitary Dysfunction as an Independent Risk Factor for Postoperative Central Nervous System Infections in Patients With Sellar Region Tumors.

Front Endocrinol (Lausanne) 2021 30;12:661305. Epub 2021 Apr 30.

Departments of Neurosurgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Objective: The purpose of this study was to verify that hypothalamus-pituitary dysfunction is one of the risk factors for postoperative central nervous system infections (PCNSIs).

Method: We performed a retrospective analysis of all patients with sellar region lesions who underwent surgery between January 2016 and November 2019 at Peking Union Medical College Hospital. In total, 44 age- and sex-matched controls were enrolled. Univariate and multivariate analyses were performed to identify risk factors for PCNSIs.

Result: We enrolled 88 patients, 44 of whom had PCNSIs. Surgical approach (TCS) (P<0.001), previous surgery on the same site (P=0.001), intraoperative cerebral spinal fluid (CSF) leakage (P<0.001), postoperative adrenal insufficiency (P=0.017), postoperative DI (P=0.004) and the maximum Na levels(<0.001) correlated significantly with PCNSIs. Multivariate analysis showed that Surgery approach (TCS)(OR: 77.588; 95%CI: 7.981-754.263; P<0.001), intraoperative CSF leakage (OR: 12.906; 95%CI: 3.499-47.602; P<0.001), postoperative DI (OR: 6.999; 95%CI:1.371-35.723; P=0.019) and postoperative adrenal insufficiency (OR: 6.115; 95%CI: 1.025-36.469; P=0.047) were independent influencing factors for PCNSIs.

Conclusion: TCS, intraoperative CSF leakage, postoperative DI and postoperative adrenal insufficiency are risk factors for PCNSIs in patients with sellar region tumors.
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http://dx.doi.org/10.3389/fendo.2021.661305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121168PMC
April 2021

MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcoma.

J Exp Clin Cancer Res 2021 May 12;40(1):166. Epub 2021 May 12.

Department of Orthopedics, Shanghai Bone Tumor Institution, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, P. R. China.

Background: Osteosarcoma (OS) is the most common primary bone cancer in adolescents and lung metastasis is the leading cause of death in patients with OS. However, the molecular mechanisms that promote OS growth and metastasis remain unknown.

Methods: We investigated the expression of myosin light chain kinase family members between metastasis and non-metastasis patients in the TARGET database and ensured that only myosin light chain kinase family member 4 (MYLK4) had higher expression in metastatic osteosarcoma patients. Then we confirmed the results by immunohistochemistry (IHC) and Western blotting (WB) of OS tissues. The effect of MYLK4 on the metastasis and proliferation of OS cells was investigated by wound healing, Transwell and colony-formation assays. Mass spectrum analysis was used to ensure the new binding protein of MYLK4. Tissue microarrays analysis was used to show the correlation between MYLK4 and pEGFR (Y1068). A series of in vivo experiments were conducted to reveal the mechanisms by which MYLK4 modulated the metastasis and proliferation of OS.

Results: Myosin Light Chain Kinase Family Member 4 (MYLK4) was significantly upregulated in metastatic human OS tissues. Growth and metastasis of OS could be accelerated by MYLK4 overexpression, whereas silencing MYLK4 expression resulted in decreased cell growth and metastasis. Mechanistically, mass spectrum analysis showed that MYLK4 interacted with the epidermal growth factor receptor (EGFR) in osteosarcoma cells and promoted growth and metastasis via the EGFR signaling pathway. Tissue microarrays analysis also showed that MYLK4 expression had a positive correlation with the expression of pEGFR (Y1068). Moreover, the EGFR inhibitor gefitinib could partially reverse the effect of cell proliferation and metastasis caused by MYLK4 overexpression. Importantly, the combination of MYLK4 and EGFR inhibitors had synergistic effects on growth and metastasis of OS in vitro and in vivo.

Conclusion: Our results indicate that MYLK4 promotes OS growth and metastasis by activating the EGFR signaling pathway and can be a novel therapeutic target for the treatment of OS patients.
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http://dx.doi.org/10.1186/s13046-021-01965-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114533PMC
May 2021

Association between ApoE status, circulating vitamin A and vitamin E levels with dyslipidemia in aging Chinese adults.

Arch Med Res 2021 May 3. Epub 2021 May 3.

School of Public Health, Capital Medical University, Beijing, PR China. Electronic address:

Background: The influence of ApoE or lipid-soluble vitamins on lipid profile has been well documented. However, the association between ApoE status, vitamin A (VA) and vitamin E (VE) with dyslipidemia has been seldom reported. The aim of the present study was to investigate the impact of ApoE status on circulating VA and VE in aging adults with dyslipidemia.

Methods: A total of 1754 Chinese aged 55-75 was recruited from community health centers. They were interviewed to obtain demographic information. Food frequency questionnaire (FFQ) was used to investigate daily food intakes of the participants. Fasting venous blood samples were taken and used for serum lipid profile measurement and ApoE genotyping. Serum VA and VE concentrations were determined by using high-performance liquid chromatography (HPLC).

Results: Serum VE and VA concentrations were circulating lipids and ApoE status dependent. Dyslipidemia subjects showed higher serum TC, TG, HDL-c/LDL-c ratio, VE and lipid-adjusted VE levels than normal subjects. ApoE genotype-dependent differences in serum lipid profile, VE and VA levels were observed in both normal and dyslipidemia subjects. The relationship between circulating VA with dyslipidemia is modifiable by lipid status.

Conclusion: Higher serum VE and lipid adjusted VE levels associated with increased risk of dyslipidemia in aging Chinese adults, especially in ApoE4 carriers. Large scale longitudinal study is required to determine the optimal circulating VE levels in the elderly based on different lipid profiles and ApoE status.
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http://dx.doi.org/10.1016/j.arcmed.2021.04.007DOI Listing
May 2021

Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.

Aging (Albany NY) 2021 05 5;13(9):12896-12918. Epub 2021 May 5.

Department of Orthopedics, Shanghai Bone Tumor Institution, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China.

Glycolysis ensures energy supply to cancer cells, thereby facilitating tumor progression. Here, we identified glycolysis-related genes that could predict the prognosis of patients with osteosarcoma. We examined 198 glycolysis-related genes that showed differential expression in metastatic and non-metastatic osteosarcoma samples in the TARGET database, and identified three genes (P4HA1, ABCB6, and STC2) for the establishment of a risk signature. Based on the signature, patients in the high-risk group had poor outcomes. An independent Gene Expression Omnibus database GSE21257 was selected as the validation cohort. Receiver operating characteristic curve analysis was performed and the accuracy of predicting the 1- and 3-year survival rates was shown by the areas under the curve. The results were 0.884 and 0.790 in the TARGET database, and 0.740 and 0.759 in the GSE21257, respectively. Furthermore, we applied ESTIMATE algorithm and performed single sample gene set enrichment analysis to compare tumor immunity between high- and low-risk groups. We found that the low-risk group had higher immune scores and immune infiltration levels than the high-risk group. Finally, we chose P4HA1 as a representative gene to verify the function of risk genes and and found that P4HA1 could promote the metastasis of osteosarcoma cells. Our study established a novel glycolysis-related risk signature that could predict the prognosis of patients with osteosarcoma.
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http://dx.doi.org/10.18632/aging.202958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148463PMC
May 2021

Modifiable risk factors of acute kidney injury after liver transplantation: a systematic review and meta-analysis.

BMC Nephrol 2021 Apr 23;22(1):149. Epub 2021 Apr 23.

Department of Anesthesiology, The Affiliated Hospital of Qingdao University, School of Clinical Medicine, Qingdao University, No. 59, Haier Road, Qingdao, 266100, Shandong Province, China.

Background: Acute kidney injury (AKI) is a common and critical complication of liver transplantation (LT), which is associated with increased morbidity, mortality and health care cost. We aimed to identify modifiable risk factors of AKI after LT.

Methods: A literature search of Pubmed, EMBASE and Cochrane Databases was performed to identify studies investigating risk factors of AKI after LT. The Newcastle-Ottawa Scale was used to rate study quality. Effect size and 95% confidence interval were pooled using a random-effect model with inverse-variance method.

Results: Sixty-seven articles with 28,844 patients were included in the meta-analysis. Seventeen modifiable risk factors were found, including overweight, preoperative use of diuretic, preoperative anemia, donation after cardiac death organ, donor BMI ≥ 30 kg/m, ABO-incompatible LT, low graft to recipient body weight ratio, intraoperative hypotension, major bleeding, intraoperative use of vasopressor, large RBC transfusion, postreperfusion syndrome, postoperative use of vasopressors, overexposure to calcineurin inhibitor, calcineurin inhibitor without mycophenolate mofetil, graft dysfunction and infection. A total of 38 articles were included in the systematic review, in which 8 modifiable risk factors and 1 protective factor were additionally associated in single studies with the incidence of AKI after LT.

Conclusions: Effective interventions based on identified modifiable risk factors in the perioperative management and graft allocation and preservation may be promising to reduce the incidence of AKI after LT.

Trial Registration: The protocol for this systematic review is registered with PROSPERO (No. CRD42020166918 ).
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http://dx.doi.org/10.1186/s12882-021-02360-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063403PMC
April 2021

Creating biotransformation of volatile fatty acids and octanoate as co-substrate to high yield medium-chain-length polyhydroxyalkanoate.

Bioresour Technol 2021 Jul 23;331:125031. Epub 2021 Mar 23.

College of Light Industry Science and Engineering, Tianjin University of Science & Technology, Tianjin 300222, PR China.

Using mixed microbial consortium (MMC) to accumulate polyhydroxyalkanoate (PHA) is an effective strategy to solve high production cost and reduce the amount of excess sludge. In this study, a process for the production of short-chain-length and medium-chain-length PHA using volatile fatty acids (VFAs) from pretreated wood hydrolysate synergistic with octanoate as co-substrate was proposed. The effects of co-substrate ratios on PHA accumulation ability and physical properties were investigated. The incorporation of co-substrate accelerated the time of PHA and 3-hydroxyoctanoate reaching the maximum production (1834 and 280 mg COD/L). The highest PHA content was 53.0% (w/w), which was equivalent to that reported previously. The biopolymer films possessed high tensile strength, Young's modulus, and could be used in the field of water vapor barrier requirements. The accumulation strategy applied for converting fermentation products VFAs and octanoate co-substrate into high value and yield PHA could potentially demonstrate the valuable for low-cost large-scale production.
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http://dx.doi.org/10.1016/j.biortech.2021.125031DOI Listing
July 2021

Diluted-CdS Quantum Dot-Assisted SnO Electron Transport Layer with Excellent Conductivity and Suitable Band Alignment for High-Performance Planar Perovskite Solar Cells.

ACS Appl Mater Interfaces 2021 Apr 31;13(14):16326-16335. Epub 2021 Mar 31.

College of Mechanical Engineering, JiLin Engineering Normal University, Changchun 130052, PR China.

An electron transport layer (ETL) with excellent conductivity and suitable band alignment plays a key role in accelerating charge extraction and transfer for achieving highly efficient planar perovskite solar cells (PSCs). Herein, a novel diluted-cadmium sulfide quantum dot (CdS QD)-assisted SnO ETL has been developed with a low-temperature fabrication process. The slight addition of CdS QDs first enhances the crystallinity and flatness of SnO ETLs so that it provides a promising workstation to obtain high-quality perovskite absorption layers. It also amazingly increases the conductivity of the SnO ETL by an order of magnitude and regulates the energy level matching between the SnO ETL and perovskite. These outstanding properties greatly accelerate the charge extraction and transfer. Thus, the MAPbI-based PSCs with such a diluted-CdSQD-assisted SnO ETL achieve a maximum power conversion efficiency of 20.78% and obtain a better stability of devices in air. These findings testify the importance and potential of semiconductor QD modification on ETLs, which may pave the way for developing such composite ETLs for further enhancing photovoltaic performance of planar PSCs.
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http://dx.doi.org/10.1021/acsami.1c00896DOI Listing
April 2021

MALAT1 knockdown promoted cell viability and migration of LPS-treated MG-63 cells via sponging miR-212.

Genes Genomics 2021 May 16;43(5):523-531. Epub 2021 Mar 16.

Department of Osteology, Tianjin Hospital, No. 406, Jiefang South Road, Tianjin, 300211, China.

Background: Most fractures could heal after treatment, around 5-10 % of patients still develop delayed union and nonunion. Evidence has increasingly shown that abnormal expression of long noncoding RNAs is closely related to the occurrence and development of various diseases including fracture healing. However, evidence regarding the effect of MALAT1 on fracture healing remains limited.

Objectives: In this study, we attempt to explore the role of MALAT1 during the process of femoral neck fracture healing and elucidate the underlying mechanism of this disease.

Methods: We first detect the expression of lncRNAs in serums from 3 pairs of patients with delayed femoral neck fracture healing and healthy volunteers using lncRNA microarray. And the expression of long noncoding RNA MALAT1 in serums and LPS-treated MG-63 cells was measured using qRT-PCR. CCK-8 assay, cell migration and qRT-PCR were applied to the role of MALAT1 knockdown in LPS-treated MG-63 cells. ELISA was used for the measurement of inflammatory cytokines in serums of patients and healthy volunteers. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism.

Results: MALAT1 expression was up-regulated in serum of patients with delayed union of femoral neck fracture. MALAT1 knockdown promoted cell viability and migration, reduced inflammation in LPS-treated MG-63 cells. The bioinformatics analysis showed MALAT1 acts as a molecular sponge for miR-212. And SOX6 was a target of miR-212. Besides, MALAT1 knockdown suppressed SOX6 expression via targeting miR-212 in LPS-treated MG-63 cells.

Conclusions: These data suggest MALAT1 knockdown promoted the biological behavior of LPS-treated MG-63 cells via sponging miR-212, which may provide a new therapeutic avenue for delayed union of femoral neck fracture.
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http://dx.doi.org/10.1007/s13258-021-01038-7DOI Listing
May 2021

EHR2CCAS: A framework for mapping EHR to disease knowledge presenting causal chain of disorders - chronic kidney disease example.

J Biomed Inform 2021 03 4;115:103692. Epub 2021 Feb 4.

Department of Biomedical Informatics, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Department of Healthcare Information Management, The University of Tokyo Hospital, Tokyo, Japan.

Objective: The goal of this work was to capture diseases in patients by comprehending the fine-grained medical conditions and disease progression manifested by transitions in medical conditions. We realize this by introducing our earlier work on a state-of-the-art knowledge presentation, which defines a disease as a causal chain of abnormal states (CCAS). Here, we propose a framework, EHR2CCAS, for constructing a system to map electronic health record (EHR) data to CCAS.

Materials And Methods: EHR2CCAS is a framework consisting of modules that access heterogeneous EHR to estimate the presence of abnormal states in a CCAS for a patient in a given time window. EHR2CCAS applies expert-driven (rule-based) and data-driven (machine learning) methods to identify abnormal states from structured and unstructured EHR data. It features data-driven approaches for unlocking clinical texts and imputations based on the EHR temporal properties and the causal CCAS structure. This study presents the CCAS of chronic kidney disease as an example. A mapping system between the EHR from the University of Tokyo Hospital and CCAS of chronic kidney disease was constructed and evaluated against expert annotation.

Results: The system achieved high prediction performance in identifying abnormal states that had strong agreement among annotators. Our handling of narrative varieties in texts and our imputation of the presence of an abnormal state markedly improved the prediction performance. EHR2CCAS presents patient data describing the temporal presence of abnormal states in CCAS, which is useful in individual disease progression management. Further analysis of the differentiation of transition among abnormal states outputted by EHR2CCAS can contribute to detecting disease subtypes.

Conclusion: This work represents the first step toward combining disease knowledge and EHR to extract abnormality related to a disease defined as fine-grained abnormal states and transitions among them. This can aid in disease progression management and deep phenotyping.
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http://dx.doi.org/10.1016/j.jbi.2021.103692DOI Listing
March 2021

Phylogenetic analysis of the complete chloroplast genome of .

Mitochondrial DNA B Resour 2020 May 22;5(3):2141-2142. Epub 2020 May 22.

Key Laboratory of Marine Eco-Environmental Science and Technology, First Institute of Oceanography, the Ministry of Natural Resources, Qingdao, China.

In this study, we sequenced and annotated the complete chloroplast genome of (GenBank accession number: MN853882). The total length of the chloroplast genome of is 180,262 bp, including 191 protein-encoding genes, 30 tRNA genes and 3 rRNA genes. The phylogenetic tree, which is based on core genes, shows that clustered into the clade and has close genetic relationships with algae and . These data will enable a better understanding of the phylogenetic status of .
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http://dx.doi.org/10.1080/23802359.2020.1765708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782886PMC
May 2020

Empirical analysis of energy consumption transfer in China's national economy from the perspective of production and demand.

Environ Sci Pollut Res Int 2021 Apr 4;28(15):19202-19221. Epub 2021 Jan 4.

School of Urban and Regional Science (Institute of Finance and Economics), Shanghai University of Finance and Economics, Shanghai, 200433, China.

Given the context of the global energy shortage and the deterioration of the ecological environment, this paper uses industrial linkage as a starting point to deeply explore the energy consumption of different sectors and its transfer characteristics. First, a dual evaluation of energy consumption in various sectors is conducted from the perspectives of production and demand to realize an integrated analysis from the energy consumption perspective. Second, based on industrial linkage, the economic input-output life cycle assessment (EIO-LCA) model is used to quantify the net transfer of energy consumption and identify the transfer of energy consumption pressure embodied in economic activities by various sectors. Finally, the energy consumption of various sectors is decomposed, and the transfer flow of energy consumption is measured to accurately quantify the degree of linkage in the energy consumption of sectors. The results show that the current direct energy consumption intensity of various sectors in China is continuously decreasing, but this decrease is insufficient to reverse the upward trend in energy consumption demand. From the perspective of production and demand, non-energy industrial sector is a key sector for reducing energy consumption, and the intersectoral linkage between the subsectors caused by product trade flows has a greater impact on the level of direct and embodied energy consumption. Direct and embodied energy consumption in the same sector can be ranked quite differently, and their levels may not be equal. Energy sector is the main net outflow sector of energy consumption, and the level of its direct energy consumption is much higher than that of its embodied energy consumption. It is worth noting that the scale of embodied energy consumption in construction industry is much higher than the scale of direct energy consumption. Construction is the sector with the greatest net inflow of energy consumed and is the main driver of the energy consumption transfer of energy sector and non-energy industrial sector.
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http://dx.doi.org/10.1007/s11356-020-11983-7DOI Listing
April 2021

Clostridium perfringens sepsis in three patients with acute leukemia and review of the literature.

Int J Hematol 2021 Apr 2;113(4):508-517. Epub 2021 Jan 2.

Department of Hematology, Aerospace Center Hospital, No. 15 Yu Quan Road, Beijing, 100049, China.

In this study, we aimed to improve understanding of the clinical manifestations, laboratory findings, and risk factors of Clostridium perfringens sepsis in patients with acute leukemia and to analyze treatment strategies for improving prognosis. We analyzed clinical manifestations, laboratory data, diagnosis, and treatment strategies in three cases of C. perfringens sepsis in patients with acute leukemia. We also reviewed and analyzed the relevant literature, incorporating our findings into the discussion. All three patients developed septic shock with neutropenia following chemotherapy. Analysis of blood samples confirmed the presence of C. perfringens, and two patients had fulminant intravascular hemolysis and developed multiple organ dysfunction syndrome. Two patients survived and one died despite timely and full-dose antibacterial treatments, blood purification, and noninvasive positive pressure ventilation. Overall, our findings showed that C. perfringens sepsis is rare in patients with acute leukemia but progresses rapidly. A high mortality rate was observed, and patients often experienced refractory shock and intravascular hemolysis. This demonstrates the importance of early detection and diagnosis. Multimodal treatments, including fluid resuscitation, antibiotics, organ support, and blood purification, are essential for success.
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http://dx.doi.org/10.1007/s12185-020-03060-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7776304PMC
April 2021

Bifunctional, Copper-Doped, Mesoporous Silica Nanosphere-Modified, Bioceramic Scaffolds for Bone Tumor Therapy.

Front Chem 2020 9;8:610232. Epub 2020 Dec 9.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

In the traditional surgical intervention procedure, residual tumor cells may potentially cause tumor recurrence. In addition, large bone defects caused by surgery are difficult to self-repair. Thus, it is necessary to design a bioactive scaffold that can not only kill residual tumor cells but also promote bone defect regeneration simultaneously. Here, we successfully developed Cu-containing mesoporous silica nanosphere-modified β-tricalcium phosphate (Cu-MSN-TCP) scaffolds, with uniform and dense nanolayers with spherical morphology 3D printing and spin coating. The scaffolds exhibited coating time- and laser power density-dependent photothermal performance, which favored the effective killing of tumor cells under near-infrared laser irradiation. Furthermore, the prepared scaffolds favored the proliferation and attachment of rabbit bone marrow-derived mesenchymal stem cells and stimulated the gene expression of osteogenic markers. Overall, Cu-MSN-TCP scaffolds can be considered for complete eradication of residual bone tumor cells and simultaneous healing of large bone defects, which may provide a novel and effective strategy for bone tumor therapy. In the future, such Cu-MSN-TCP scaffolds may function as carriers of anti-cancer drugs or immune checkpoint inhibitors in chemo-/photothermal or immune-/photothermal therapy of bone tumors, favoring for effective treatment.
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http://dx.doi.org/10.3389/fchem.2020.610232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7755992PMC
December 2020

Comparing the effects of 12 months aerobic exercise and resistance training on glucose metabolism among prediabetes phenotype: A explorative randomized controlled trial.

Prim Care Diabetes 2021 04 10;15(2):340-346. Epub 2020 Dec 10.

Jiangsu Province Academy of Traditional Chinese Medicine, #100 Hongshan Road, Qixia District Nanjing, 210028 PR China. Electronic address:

Aims: The pathophysiology of each phenotype of prediabetes is unique that promotes different levels of diabetes and cardiovascular disease risks. Exercise guidelines for individuals with prediabetes including both aerobic and resistance training could improve metabolic control, but its effects on different prediabetes subtypes are unclear. The aim of this explorative randomized controlled trial was to evaluate the effects of aerobic training (AT) or resistance training (RT) on glucose metabolism and lipid profile by different prediabetes subtypes with.

Methods: A randomized controlled trial in which 128 individuals with isolated impaired fasting glucose (i-IFG; n = 39), isolated impaired glucose tolerance (i-IGT; n = 29), combined glucose tolerance (CGI; n = 27) and isolated elevated HbA (n = 33) were randomly assigned to the control group, AT group and RT group, respectively. Supervised exercise training, including AT and RT were completed at moderate intensity for 60 min per day, three non-consecutive days per week for 12 months. The primary outcome was improvement in glucose metabolism. Secondary outcomes included measure of lipid profile and if these effects were moderated by the prediabetes phenotype.

Results: Of the initial 128 participants, 118 finished the study, but all participants were included in the intention-to-treat analyses. The improvement in 2 h postprandial plasma glucose (2 hPG) between group difference (AT vs. RT) at 12 months was 0.87 (95% CI, -1.59 to-0.16; p < 0.05). Compared with RT group, AT significantly decreased the 2hPG in participants with i-IGT at 12 months (-1.66, 95% CI -3.04 to -0.28; p < 0.05).

Conclusions: AT program conferred benefits in improving 2 h PG and HbA compared with RT for prediabetes. These findings may moderate by prediabetes phenotype, and AT appeared more effective in i-IGT. A future trial with large sample size and long time follow up of prediabetes phenotype groups are needed.
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http://dx.doi.org/10.1016/j.pcd.2020.11.003DOI Listing
April 2021

A nomogramic model based on clinical and laboratory parameters at admission for predicting the survival of COVID-19 patients.

BMC Infect Dis 2020 Nov 30;20(1):899. Epub 2020 Nov 30.

Department of Critical Care Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan 2nd Road, Guangzhou, 510080, Guangdong, China.

Background: COVID-19 has become a major global threat. The present study aimed to develop a nomogram model to predict the survival of COVID-19 patients based on their clinical and laboratory data at admission.

Methods: COVID-19 patients who were admitted at Hankou Hospital and Huoshenshan Hospital in Wuhan, China from January 12, 2020 to March 20, 2020, whose outcome during the hospitalization was known, were retrospectively reviewed. The categorical variables were compared using Pearson's χ-test or Fisher's exact test, and continuous variables were analyzed using Student's t-test or Mann Whitney U-test, as appropriate. Then, variables with a P-value of ≤0.1 were included in the log-binomial model, and merely these independent risk factors were used to establish the nomogram model. The discrimination of the nomogram was evaluated using the area under the receiver operating characteristic curve (AUC), and internally verified using the Bootstrap method.

Results: A total of 262 patients (134 surviving and 128 non-surviving patients) were included in the analysis. Seven variables, which included age (relative risk [RR]: 0.905, 95% confidence interval [CI]: 0.868-0.944; P < 0.001), chronic heart disease (CHD, RR: 0.045, 95% CI: 0.0097-0.205; P < 0.001, the percentage of lymphocytes (Lym%, RR: 1.125, 95% CI: 1.041-1.216; P = 0.0029), platelets (RR: 1.008, 95% CI: 1.003-1.012; P = 0.001), C-reaction protein (RR: 0.982, 95% CI: 0.973-0.991; P < 0.001), lactate dehydrogenase (LDH, RR: 0.993, 95% CI: 0.990-0.997; P < 0.001) and D-dimer (RR: 0.734, 95% CI: 0.617-0.879; P < 0.001), were identified as the independent risk factors. The nomogram model based on these factors exhibited a good discrimination, with an AUC of 0.948 (95% CI: 0.923-0.973).

Conclusions: A nomogram based on age, CHD, Lym%, platelets, C-reaction protein, LDH and D-dimer was established to accurately predict the prognosis of COVID-19 patients. This can be used as an alerting tool for clinicians to take early intervention measures, when necessary.
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http://dx.doi.org/10.1186/s12879-020-05614-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7702207PMC
November 2020

High soil pH enhances the network interactions among bacterial and archaeal microbiota in alpine grasslands of the Tibetan Plateau.

Environ Microbiol 2021 01 28;23(1):464-477. Epub 2020 Nov 28.

School of Life Sciences, Lanzhou University, Lanzhou, China.

Soil functions and processes are driven by complex microbial interactions. It is, therefore, critical to understand the coexistence patterns of soil microbiota, especially in fragile alpine ecosystems. We identified biogeographic patterns in the network-level topological features of the soil microbial co-occurrence network in the Tibetan alpine grasslands, based on high-throughput sequencing. We verified that soil pH was the most important environmental variable for predicting network-level topological features of soil microbial co-occurrence networks. Associations among soil microbiota were enhanced with increasing pH (5.17-8.92), and the network was the most stable at neutral pH. Moreover, node-level topological features suggested that the archaeal operational taxonomic units, compared with bacterial operational taxonomic units, hold a central role in the co-occurrence network. Network-level topological features revealed closer connections among soil microbiota in the steppe ecosystem than in the meadow ecosystem. Therefore, our study demonstrated that soil pH served as a critical environmental filter that influenced the potential associations and ecological signature of soil microbiota in the Tibetan alpine grasslands. These findings provide a new perspective on the distinct biogeographic patterns of co-occurrence networks, to explore the ecological role of soil microbiota and thus help manage soil bacterial and archaeal communities for provisioning alpine ecosystem services.
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http://dx.doi.org/10.1111/1462-2920.15333DOI Listing
January 2021
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