Publications by authors named "Xiaojiao Liu"

25 Publications

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Plant Growth Promoting Rhizobacteria Alleviate Aluminum Toxicity and Ginger Bacterial Wilt in Acidic Continuous Cropping Soil.

Front Microbiol 2020 30;11:569512. Epub 2020 Nov 30.

College of Plant Protection, Southwest University, Chongqing, China.

Long-term monoculture cropping is usually accompanied by soil acidification and microbial community shifts. Soil aluminum ions are dissolved under acidic condition (pH < 5.0), and the resulting aluminum bioavailability can cause toxic effects in plants. In this study, we investigated the bacterial community compositions and aluminum toxicity in fields monocultured with ginger for 35 years, 15 years, and 1 year. Within these fields are ginger plants without and with ginger bacterial wilt disease. The results confirmed that the degree of aluminum toxicity in the diseased soil was more severe than that in the healthy soil. Continuous cropping can significantly increase the bacterial diversity and change the bacterial community composition of ginger rhizosphere soil. The relative abundance of plant growth-promoting rhizobacteria (PGPRs) was increased in the soils used for the continuous cropping of ginger. Additionally, aluminum toxicity had a significant positive correlation with , and in healthy soils. Based on these results, aluminum stress may stimulate the increase of PGPRs (, and ), thereby alleviating ginger aluminum toxicity and bacterial wilt in extremely acidic soil (pH < 4.5).
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http://dx.doi.org/10.3389/fmicb.2020.569512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793916PMC
November 2020

Exploration of turn-positive RT-PCR results and factors related to treatment outcome in COVID-19: A retrospective cohort study.

Virulence 2020 12;11(1):1250-1256

Department of Gastroenterology, Renmin Hospital of Wuhan University , Wuhan, P.R. China.

The cause of some patients with negative RT-PCR results experienced turn-positive after treatment remains unclear. In addition, understanding the correlation between changes in clinical data in the course of COVID-19 and treatment outcomes is of great importance in determining the prognosis of COVID-19. To perform cause analysis of RT-PCR turn-positive and the effective screening factors related to treatment outcome in COVID-19. Clinical data, including clinical manifestations, laboratory tests, radiography results, treatment methods and outcomes, were retrospectively collected and analyzed from January to March 2020 in Renmin Hospitals of Wuhan University. 116 COVID-19 patients (40 in recurrent group, 29 in recovered group and 47 in unrecovered group) were recruited. In the recurrent group, white blood cell, Neutrophils, prothrombin time, activated partial thromboplastin time, CD3, CD4, CD8, ratio of CD4/CD8, IgG and C4 complement were of significant difference among the baseline, negative and turn-positive time points. CD19 and CT scan results were found notable difference between recurrent group and recovered group. Odds from CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT scan results validated associations with clinical outcomes of COVID-19. The so-called recurrence in some COVID-19 patients may be due to the false-negative of nucleic acid test results from nasopharyngeal swabs. Levels of CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT results were significantly correlated with the outcome of COVID-19. The cellular immunity test could be beneficial to further screen the reliability of RT-PCR test on the basis of CT images.
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http://dx.doi.org/10.1080/21505594.2020.1816076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549945PMC
December 2020

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p.

Cancer Cell Int 2020 31;20:424. Epub 2020 Aug 31.

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an, 710061 Shaanxi China.

Background: Cervical cancer (CC) is the one of most common malignant gynecological tumors, which is characterized with the high mortality and recurrence rate. Previous studies have elucidated the oncogenic role of small nucleolar RNA host gene 6 (SNHG6) in some types of human cancers, whereas it is unclear whether it functions as an oncogene in CC. This study was aimed at unveiling the role of SNHG6 in CC.

Methods: qRT-PCR analysis was implemented to evaluate the expression levels of SNHG6, miR-485-3p and STYX in CC cells. RNA pull down assay and luciferase reporter assay were conducted to verify the interaction between miR-485-3p and SNHG6 or STYX. Functional assays, such as colony formation assay, JC-1 assay and TUNEL assay were applied to detect the biological behaviors of CC cells. The resistance of CC cells to radiation was evaluated by colony formation assay.

Results: SNHG6 was expressed at a high level in CC cells. Silenced SNHG6 suppressed cell proliferation but promoted cell apoptosis. Additionally, silenced SNHG6 could sensitize CC cells to radiation treatment. miR-485-3p could bind to both SNHG6 and STYX. Knockdown of miR-485-3p or overexpression of STYX could abolish the effects of SNHG6 silencing on CC cell growth.

Conclusions: LncRNA SNHG6 enhances the radioresistance of CC cells and promotes CC cell growth by sponging miR-485-3p to release STYX.
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http://dx.doi.org/10.1186/s12935-020-01448-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457785PMC
August 2020

Methylation in combination with temperature programming enables rapid identification of polysaccharides by ambient micro-fabrication glow discharge plasma (MFGDP) desorption ionization mass spectrometry.

Talanta 2020 Oct 15;218:121156. Epub 2020 May 15.

Research Center of Analytical Instrumentation, Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi, 710069, China. Electronic address:

Obtaining the fingerprints of polysaccharides was known to be notoriously difficult by ambient mass spectrometry due to their resistance for desorption and ionization. Ambient mass spectrometry technology has recently been recognized as a quick analysis tool for obtaining fingerprints, which is attributed to its characteristics of no sample pretreatment and easy operation under atmospheric pressure. However, it still remains a challenge for accurate identification of the fingerprints of macromolecular polysaccharides by ambient micro-fabrication glow discharge plasma (MFGDP). In this study, a simplified methylation method was introduced to realize rapid analysis of polysaccharide mixture by MFGDP with the assistance of a temperature-programmed system (TPS). At the optimal temperature of TPS, oligosaccharides, plant polysaccharides and polysaccharide mixtures were all well characterized by TPS-MFGDP. In the proposed method, the characteristic [M + NH] adduct ions of oligosaccharides ions of oligosaccharides that are difficult for other ambient mass spectrometric methods were abundantly produced, making it possible to simultaneously identify a mixture of five or more polysaccharides. In addition, a supervised classification model which based on MS spectra, was used to classify seven typical hypoglycemic polysaccharides with excellent sensitivity, specificity and accuracy, indicating the good classification performance of the RF model constructed. Thus, the proposed mass spectroscopic method provides a cost effective, accurate and high throughput tool for identification and classification of polysaccharides, which is beneficial for studying the biological activity of polysaccharides.
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http://dx.doi.org/10.1016/j.talanta.2020.121156DOI Listing
October 2020

Experimental and simulation study of the high-pressure behavior of squalane and poly-α-olefins.

J Chem Phys 2020 Feb;152(7):074504

School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh EH9 3FJ, Scotland.

The equation of state, dynamical properties, and molecular-scale structure of squalane and mixtures of poly-α-olefins at room temperature are studied with a combination of state-of-the-art, high-pressure experiments and molecular-dynamics simulations. Diamond-anvil cell experiments indicate that both materials are non-hydrostatic media at pressures above ∼1 GPa. The equation of state does not exhibit any sign of a first-order phase transition. High-pressure x-ray diffraction experiments on squalane show that there are no Bragg peaks, and hence, the apparent solidification occurs without crystallization. These observations are complemented by a survey of the equation of state and dynamical properties using simulations. The results show that molecular diffusion is essentially arrested above about 1 GPa, which supports the hypothesis that the samples are kinetically trapped in metastable amorphous-solid states. The shear viscosity becomes extremely large at very high pressures, and the coefficient governing its increase from ambient pressure is in good agreement with the available literature data. Finally, simulated radial distribution functions are used to explore the evolution of the molecular-scale structure with increasing pressure. Subtle changes in the short-range real-space correlations are related to a collapse of the molecular conformations with increasing pressure, while the evolution of the static structure factor shows excellent correlation with the available x-ray diffraction data. These results are of indirect relevance to oil-based lubricants, as the pressures involved are comparable to those found in engines, and hence, the ability of lubricating thin films to act as load-bearing media can be linked to the solidification phenomena studied in this work.
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http://dx.doi.org/10.1063/1.5139723DOI Listing
February 2020

Structural basis of transcription inhibition by the DNA mimic protein Ocr of bacteriophage T7.

Elife 2020 02 10;9. Epub 2020 Feb 10.

Section of Structural Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, London, United Kingdom.

Bacteriophage T7 infects and evades the host restriction/modification system. The Ocr protein of T7 was shown to exist as a dimer mimicking DNA and to bind to host restriction enzymes, thus preventing the degradation of the viral genome by the host. Here we report that Ocr can also inhibit host transcription by directly binding to bacterial RNA polymerase (RNAP) and competing with the recruitment of RNAP by sigma factors. Using cryo electron microscopy, we determined the structures of Ocr bound to RNAP. The structures show that an Ocr dimer binds to RNAP in the cleft, where key regions of sigma bind and where DNA resides during transcription synthesis, thus providing a structural basis for the transcription inhibition. Our results reveal the versatility of Ocr in interfering with host systems and suggest possible strategies that could be exploited in adopting DNA mimicry as a basis for forming novel antibiotics.
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http://dx.doi.org/10.7554/eLife.52125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064336PMC
February 2020

Volatile-mediated antagonism of soil bacterial communities against fungi.

Environ Microbiol 2020 03 4;22(3):1025-1035. Epub 2019 Nov 4.

Department of Microbial Ecology, Netherlands Institute of Ecology, NIOO-KNAW, Wageningen, 6708 PB, The Netherlands.

Competition is a major type of interaction between fungi and bacteria in soil and is also an important factor in suppression of plant diseases caused by soil-borne fungal pathogens. There is increasing attention for the possible role of volatiles in competitive interactions between bacteria and fungi. However, knowledge on the actual role of bacterial volatiles in interactions with fungi within soil microbial communities is lacking. Here, we examined colonization of sterile agricultural soils by fungi and bacteria from non-sterile soil inoculums during exposure to volatiles emitted by soil-derived bacterial communities. We found that colonization of soil by fungi was negatively affected by exposure to volatiles emitted by bacterial communities whereas that of bacteria was barely changed. Furthermore, there were strong effects of bacterial community volatiles on the assembly of fungal soil colonizers. Identification of volatile composition produced by bacterial communities revealed several compounds with known fungistatic activity. Our results are the first to reveal a collective volatile-mediated antagonism of soil bacteria against fungi. Given the better exploration abilities of filamentous fungi in unsaturated soils, this may be an important strategy for bacteria to defend occupied nutrient patches against invading fungi. Another implication of our research is that bacterial volatiles in soil atmospheres can have a major contribution to soil fungistasis.
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http://dx.doi.org/10.1111/1462-2920.14808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064993PMC
March 2020

Exogenous Calcium Ions Enhance Patulin Adsorption Capability of .

J Food Prot 2019 Aug;82(8):1390-1397

1 College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi, 710119, People's Republic of China.

Patulin contamination is a serious issue that restricts the development of the global fruit processing industry. Yeasts adsorb patulin more effectively than other microbial adsorbents, and this adsorption process depends mainly on the function of the cell wall. The present study examined the effect of exogenous calcium, in concentrations ranging from 0 to 1 mol/L, on cell wall structure and on patulin adsorption capability; the patulin adsorption capability of yeast was found to strengthen with an increase in exogenous calcium concentrations from 1 × 10 to 1 × 10 mol/L. Moreover, yeast cell wall thickness, β-1,3-glucan content, and the activities of the key catalytic enzymes β-1,3-glucanase and β-1,3-glycosyl transferase were all increased within this range. The results indicate that exogenous calcium activates key enzymes that are crucial for cell wall network formation and patulin adsorption capability.
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http://dx.doi.org/10.4315/0362-028X.JFP-18-496DOI Listing
August 2019

An acetonitrile-solvated cocrystal of piroxicam and succinic acid with co-existing zwitterionic and non-ionized piroxicam molecules.

Acta Crystallogr C Struct Chem 2019 01 1;75(Pt 1):29-37. Epub 2019 Jan 1.

Boreskov Institute of Catalysis, Siberian Branch of Russian Academy of Sciences, pr. Lavrentieva 5, Novosibirisk 630090, Russian Federation.

This work reports a new acetonitrile (ACN)-solvated cocrystal of piroxicam (PRX) and succinic acid (SA), 2CHNOS·0.5CHO·CHN or PRX:SA:ACN (4:1:2), which adopts the triclinic space group P-1. The outcome of crystallization from ACN solution can be controlled by varying only the PRX:SA ratio, with a higher PRX:SA ratio in solution unexpectedly favouring a lower stoichiometric ratio in the solid product. In the new solvate, zwitterionic (Z) and non-ionized (NI) PRX molecules co-exist in the asymmetric unit. In contrast, the nonsolvated PRX-SA cocrystal contains only NI-type PRX molecules. The ACN molecule entrapped in PRX-SA·ACN does not form any hydrogen bonds with the surrounding molecules. In the solvated cocrystal, Z-type molecules form dimers linked by intermolecular N-H...O hydrogen bonds, whereas every pair of NI-type molecules is linked to SA via N-H...O and O-H...N hydrogen bonds. Thermogravimetry and differential scanning calorimetry suggest that thermal desolvation of the solvate sample occurs at 148 °C, and is followed by recrystallization, presumably of a multicomponent PRX-SA structure. Vibrational spectra (IR and Raman spectroscopy) of PRX-SA·ACN and PRX-SA are also used to demonstrate the ability of spectroscopic techniques to distinguish between NI- and Z-type PRX molecules in the solid state. Hence, vibrational spectroscopy can be used to distinguish the PRX-SA cocrystal and its ACN solvate.
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http://dx.doi.org/10.1107/S2053229618016911DOI Listing
January 2019

Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field.

Appl Microbiol Biotechnol 2018 Nov 9;102(22):9781-9791. Epub 2018 Oct 9.

Laboratory of Natural Products Pesticides, Plant Protection College of Southwest University, Chongqing, 400715, China.

Application of soil amendments has been wildly used to increase soil pH and control bacterial wilt. However, little is known about causal shifts in the rhizosphere microbial community of crops, especially when the field naturally harbors the disease of bacterial wilt to tobacco for many years due to long-term continuous cropping and soil acidification. In this study, biochar (CP), lime (LM), oyster shell powder (OS) and no soil amendment additions (Control; CK) were assessed for their abilities to improve the soil acidification, change the composition of rhizosphere soil bacterial communities and thus control tobacco bacterial wilt. The results showed that oyster shell powder significantly increased soil pH by 0.77 and reduced the incidence of tobacco bacterial wilt by 36.67% compared to the control. The Illumina sequencing -based community analysis showed that soil amendment applications affected the composition of rhizosphere bacterial community and increased the richness and diversity. In contrast, the richness and diversity correlated negatively to disease incidence. Using LEfSe analyses, 11 taxa were found to be closely related with disease suppression, in which Saccharibacteria, Aeromicrobium, and Pseudoxanthomonas could be potential indicators of disease suppression. Our results suggested that the suppression of bacterial wilt after the application of soil amendments (especially oyster shell powder) was attributed to the improved soil pH and increased bacterial richness and diversity.
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http://dx.doi.org/10.1007/s00253-018-9347-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6208964PMC
November 2018

Legacy effects of continuous chloropicrin-fumigation for 3-years on soil microbial community composition and metabolic activity.

AMB Express 2017 Sep 18;7(1):178. Epub 2017 Sep 18.

Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, No.2 Tiansheng Road, Beibei, Chongqing, 400715, China.

Chloropicrin is widely used to control ginger wilt in China, which have an enormous impact on soil microbial diversity. However, little is known on the possible legacy effects on soil microbial community composition with continuous fumigation over different years. In this report, we used high throughput Illumina sequencing and Biolog ECO microplates to determine the bacterial community and microbial metabolic activity in ginger harvest fields of non-fumigation (NF), chloropicrin-fumigation for 1 year (F_1) and continuous chloropicrin-fumigation for 3 years (F_3). The results showed that microbial richness and diversity in F_3 were the lowest, while the metabolic activity had no significant difference. With the increase of fumigation years, the incidence of bacterial wilt was decreased, the relative abundance of Actinobacteria and Saccharibacteria were gradually increased. Using LEfSe analyses, we found that Saccharibacteria was the most prominent biomarker in F_3. Eight genera associated with antibiotic production in F_3 were screened out, of which seven belonged to Actinobacteria, and one belonged to Bacteroidetes. The study indicated that with the increase of fumigation years, soil antibacterial capacity may be increased (possible reason for reduced the incidence of bacterial wilt), and Saccharibacteria played a potential role in evaluating the biological effects of continuous fumigation.
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http://dx.doi.org/10.1186/s13568-017-0475-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603465PMC
September 2017

Using community analysis to explore bacterial indicators for disease suppression of tobacco bacterial wilt.

Sci Rep 2016 11 18;6:36773. Epub 2016 Nov 18.

Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing, China.

Although bacterial communities play important roles in the suppression of pathogenic diseases and crop production, little is known about the bacterial communities associated with bacterial wilt. Based on 16S rRNA gene sequencing, statistical analyses of microbial communities in disease-suppressive and disease-conducive soils from three districts during the vegetation period of tobacco showed that Proteobacteria was the dominant phylum, followed by Acidobacteria. Only samples from September were significantly correlated to disease factors. Fifteen indicators from taxa found in September (1 class, 2 orders, 3 families and 9 genera) were identified in the screen as being associated with disease suppression, and 10 of those were verified for potential disease suppression in March. Kaistobacter appeared to be the genus with the most potential for disease suppression. Elucidating microbially mediated natural disease suppression is fundamental to understanding microecosystem responses to sustainable farming and provides a possible approach for modeling disease-suppressive indicators. Here, using cluster analysis, MRPP testing, LEfSe and specific filters for a Venn diagram, we provide insight into identifying possible indicators of disease suppression of tobacco bacterial wilt.
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http://dx.doi.org/10.1038/srep36773DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114674PMC
November 2016

Purification and characterization of a novel bacteriocin produced by Lactobacillus crustorum MN047 isolated from koumiss from Xinjiang, China.

J Dairy Sci 2016 Sep 8;99(9):7002-7015. Epub 2016 Aug 8.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi Province 712100, China. Electronic address:

The growing emergence of antibiotic-resistant bacteria in the food industry needs to be controlled with effective antimicrobials. In this study, bacteriocin MN047 A (BMA) was found to have antibacterial activity against multidrug-resistant bacteria. It was produced by Lactobacillus crustorum MN047, which was first isolated from koumiss, a traditional fermented dairy product from Xinjiang Autonomous Region, China. It was purified by ammonium sulfate precipitation, ion-exchange chromatography, and reversed-phase chromatography. It had a low molecular mass of 1,770.89 Da according to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the sequence was identified as QLPWQILGIVAGMFQA by liquid chromatography-tandem mass spectrometry analysis and MASCOT searching. It was proteinaceous in nature: the bacteriocin was digested by protease but not by α-amylase or lipase. It showed broad pH toleration (pH 2-11), good thermostability, and good storage stability. It had a broad inhibitory spectrum, including both gram-positive and gram-negative bacteria. Growth curve and time-kill kinetics indicated that it was bactericidal to the indicator strains, and this finding was verified by scanning electron microscope and transmission electron microscope after treatment with BMA. As well, BMA halted the growth of Staphylococcus aureus and Escherichia coli in the G1 and G2/M phases according to cell-cycle analysis by flow cytometry, indicating that BMA had comprehensive inhibitory effects against foodborne pathogens.
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http://dx.doi.org/10.3168/jds.2016-11166DOI Listing
September 2016

Breast metastasis from signet ring cell carcinoma of the urinary bladder: A case report.

Mol Clin Oncol 2016 Jul 28;5(1):111-112. Epub 2016 Apr 28.

Department of Breast Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.

Carcinoma of the bladder metastatic to the breast is only sporadically reported in the literature. To the best of our knowledge, the present report is the first described case of signet ring cell carcinoma of the urinary bladder metastasizing to the breast. The patient was a 43-year-old woman who underwent transurethral partial cystectomy for signet ring cell carcinoma of the urinary bladder and adjuvant chemotherapy with cisplatin and gemcitabine. At 7 months postcystectomy, the patient presented with a solitary nodule in the right breast. Following transdermic core needle puncture biopsy of the lesion and histological examination, the tumor was found to be composed of signet ring cells, which were similar to the cells in the original cystectomy specimen. The patient underwent mastectomy without further chemotherapy and has remained free from metastasis to other organs during 1 year follow-up.
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http://dx.doi.org/10.3892/mco.2016.884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4907064PMC
July 2016

Prognostic value of preoperative inflammatory markers in Chinese patients with breast cancer.

Onco Targets Ther 2014 26;7:1743-52. Epub 2014 Sep 26.

Department of Breast Center, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, People's Republic of China.

Cancer-associated inflammation is a key determinant of disease progression and survival in most cancers. The aim of our study was to assess the predictive value of preoperative inflammatory markers, such as the neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio, red cell distribution width (RDW), and mean platelet volume, for survival in breast cancer patients. In total, 608 breast cancer patients operated on between January 2009 and December 2011 were included in this observational study. The association between preoperative inflammatory markers and survival outcomes was analyzed. Patients with high NLR (>2.57) or high RDW (>13.45%) showed a significantly lower overall survival rate than those with lower NLR (≤2.57) or lower RDW (≤13.45%). NLR and RDW, along with node stage and molecular subtypes, were independent prognostic factors. There was a significant survival difference according to NLR in the luminal A and triple-negative subtypes (93.3% versus 99.3%, P=0.001; 68.8% versus 95.1%, P=0.000, respectively). The triple-negative subtype was the only subtype in which higher RDW patients showed significantly poor prognosis (81.3% versus 95.5%, P=0.025). Pre-operation NLR and RDW is a convenient, easily measured prognostic indicator for patients with breast cancer, especially in patients with the triple-negative subtype.
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http://dx.doi.org/10.2147/OTT.S69657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196795PMC
October 2014

Development of novel microsatellite markers for the BBCC Oryza genome (Poaceae) using high-throughput sequencing technology.

PLoS One 2014 14;9(3):e91826. Epub 2014 Mar 14.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.

Wild species of Oryza are extremely valuable sources of genetic material that can be used to broaden the genetic background of cultivated rice, and to increase its resistance to abiotic and biotic stresses. Until recently, there was no sequence information for the BBCC Oryza genome; therefore, no special markers had been developed for this genome type. The lack of suitable markers made it difficult to search for valuable genes in the BBCC genome. The aim of this study was to develop microsatellite markers for the BBCC genome. We obtained 13,991 SSR-containing sequences and designed 14,508 primer pairs. The most abundant was hexanuclelotide (31.39%), followed by trinucleotide (27.67%) and dinucleotide (19.04%). 600 markers were selected for validation in 23 accessions of Oryza species with the BBCC genome. A set of 495 markers produced clear amplified fragments of the expected sizes. The average number of alleles per locus (Na) was 2.5, ranging from 1 to 9. The genetic diversity per locus (He) ranged from 0 to 0.844 with a mean of 0.333. The mean polymorphism information content (PIC) was 0.290, and ranged from 0 to 0.825. Of the 495 markers, 12 were only found in the BB genome, 173 were unique to the CC genome, and 198 were also present in the AA genome. These microsatellite markers could be used to evaluate the phylogenetic relationships among different Oryza genomes, and to construct a genetic linkage map for locating and identifying valuable genes in the BBCC genome, and would also for marker-assisted breeding programs that included accessions with the AA genome, especially Oryza sativa.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091826PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3954799PMC
December 2014

Lin28 mediates radiation resistance of breast cancer cells via regulation of caspase, H2A.X and Let-7 signaling.

PLoS One 2013 20;8(6):e67373. Epub 2013 Jun 20.

Key Laboratory of Biotherapy of Zhejiang Province, Department of Surgical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Resistance to radiation therapy is a major obstacle for the effective treatment of cancers. Lin28 has been shown to contribute to breast tumorigenesis; however, the relationship between Lin28 and radioresistance remains unknown. In this study, we investigated the association of Lin28 with radiation resistance and identified the underlying mechanisms of action of Lin28 in human breast cancer cell lines. The results showed that the expression level of Lin28 was closely associated with resistance to radiation treatment. The T47D cancer cell line, which highly expresses Lin28, is more resistant to radiation than MCF7, Bcap-37 or SK-BR-3 cancer cell lines, which have low-level Lin28 expression. Transfection with Lin28 siRNA significantly led to an increase of sensitivity to radiation. By contrast, stable expression of Lin28 in breast cancer cells effectively attenuated the sensitivity to radiation treatment. Stable expression of Lin28 also significantly inhibited radiation-induced apoptosis. Moreover, further studies have shown that caspases, H2A.X and Let-7 miRNA were the molecular targets of Lin28. Stable expression of Lin28 and treatment with radiation induced H2AX expression, while inhibited p21 and γ-H2A.X. Overexpression of Let-7 enhanced the sensitivities to radiation in breast cancer cells. Taken together, these results indicate that Lin28 might be one mechanism underlying radiation resistance, and Lin28 could be a potential target for overcoming radiation resistance in breast cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067373PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688678PMC
February 2014

Analysis of factors related to patient peritoneal dialysis drop out rates.

Saudi Med J 2013 Apr;34(4):428-31

First College of Clinical Medical Science of China, Three Gorges University, Yichang City, Hubei Province, China.

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April 2013

Disruption of precise regulation of αPKC expression and cellular localization is associated with cervical cancer progression.

Arch Gynecol Obstet 2013 Aug 27;288(2):401-8. Epub 2013 Feb 27.

Department of Gynecology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.

Purpose: To understand the pathogenesis of cervical cancer (CC) associated with polarity protein αPKC and the potential roles of αPKC in clinical management of CC.

Methods: Tissue samples were collected from women who received colposcopy biopsy or hysterectomy surgery, including 9 CIN1, 8 CIN2, 15 CIN3, and 12 invasive cervical squamous cancer (ICC). 16 normal controls were from the normal region of tumor samples, HE and immunofluorescence staining of αPKC were performed on these samples. ANOVA and Kruslal-wallis test were used to quantitate the abnormal distribution and expression level of αPKC among different cervical lesions.

Results: Disruption of polarized apical localization and increased cytoplasmic accumulation of αPKC were identified in cervical lesions. In normal cervical epithelium, αPKC was detected on the apical membrane of endocervical columnar epithelial cells and of exocervical epithelial cells located at basal layer of squamous epithelium. While in squamous metaplasia, a precancerous lesion of cervical neoplasia, the polarized apical membrane localization of αPKC was disrupted, and intensed cytoplasmic accumulation was identified in the immature squamous metaplastic cells. Compared with normal cervix, number of epithelial cells with abnormal αPKC distribution was progressively increased in CINs and ICC (P < 0.05), and cytoplasmic accumulation of αPKC was increased in CIN2, CIN3, and ICC compared with CIN1 (P < 0.05).

Conclusions: Disruption of polarized apical localization and increased cytoplasmic accumulation of αPKC were associated with CC progression, indicating that precise regulation of αPKC may play important roles in CC progression, and αPKC may be a potential molecular target for clinical diagnoses and treatment of CC.
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http://dx.doi.org/10.1007/s00404-013-2770-7DOI Listing
August 2013

Methylenetetrahydrofolate reductase (MTHFR) polymorphisms and susceptibility for cervical lesions: a meta-analysis.

PLoS One 2012 21;7(12):e52381. Epub 2012 Dec 21.

Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China.

Background: The association between the methylenetetrahydrofolate reductase (MTHFR) C677T/A1298C polymorphisms and the susceptibility to cervical lesions was unclear. This study was designed to investigate their precise association using a large-scale meta-analysis.

Methods: The previous 16 studies were identified by searching PubMed, Embase and CBM databases. The crude odds ratios and their corresponding 95% confidence intervals (CIs) were used to estimate the association between the MTHFR C677T/A1298C polymorphisms and the susceptibility to the cervical lesions. The subgroup analyses were made on the following: pathological history, geographic region, ethnicity, source of controls and source of DNA for genotyping.

Results: Neither of the polymorphisms had a significant association with the susceptibility to the cervical lesions in all genetic models. Similar results were found in the subgroup analyses. No association was found between the MTHFR C677T polymorphism and the cervical lesions in the Asia or the America populations though a significant inverse association was found in the Europe population (additive model: P = 0.006, OR = 0.83, 95% CI = 0.72-0.95; CT vs. CC: P = 0.05, OR = 0.83, 95% CI = 0.69-1.00; TT vs. CC: P = 0.05, OR = 0.73, 95% CI = 0.53-1.00). Interestingly, women with the MTHFR A1298C polymorphisms had a marginally increased susceptibility to invasive cancer (ICC) when compared with no carriers but no statistically significant difference in the dominant model (P = 0.06, OR = 1.21, 95% CI = 0.99-1.49) and AC vs. AA (P = 0.09, OR = 1.21, 95% CI = 0.97-1.51).

Conclusions: The MTHFR C677T and A1298C polymorphisms may not increase the susceptibility to cervical lesions. However, the meta-analysis reveals a negative association between the MTHFR C677T polymorphisms and the cervical lesions, especially in the European populations. The marginal association between the MTHFR A1298C polymorphisms and the susceptibility to cervical cancer requires a further study.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052381PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528671PMC
June 2013

Cruciferous vegetables intake is inversely associated with risk of breast cancer: a meta-analysis.

Breast 2013 Jun 9;22(3):309-13. Epub 2012 Aug 9.

Department of Gastrointestinal Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou, Zhejiang Province, China.

Purpose: The objective of the study was to examine the associations of cruciferous vegetables intake with risk of breast cancer.

Methods: Studies were identified by searching PubMed databases and screening the references of retrieved articles and reviews. Summary odds ratios (ORs) for the highest versus lowest cruciferous vegetables consumption levels were calculated using fixed or random effects models depending on heterogeneity between studies. Heterogeneity among studies was examined using Q and I(2) statistics. Publication bias was assessed using the Egger's and Begg's tests.

Results: Thirteen epidemiologic studies (11 case-control and 2 cohort studies) were included in the meta-analysis. The combined results from all studies indicated that high cruciferous vegetables intake was significantly associated with reduced breast cancer risk (RR = 0.85, 95% CI = 0.77-0.94).

Conclusion: Findings from this meta-analysis suggest that cruciferous vegetables consumption may reduce the risk of breast cancer. Because of the limited number of studies, further prospective studies are needed to explore the protective effect of cruciferous vegetables on breast cancer.
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http://dx.doi.org/10.1016/j.breast.2012.07.013DOI Listing
June 2013

Lin28 mediates paclitaxel resistance by modulating p21, Rb and Let-7a miRNA in breast cancer cells.

PLoS One 2012 9;7(7):e40008. Epub 2012 Jul 9.

Department of Gastrointestinal Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.

Resistance to chemotherapy is a major obstacle for the effective treatment of cancers. Lin28 has been shown to contribute to tumor relapse after chemotherapy; however, the relationship between Lin28 and chemoresistance remained unknown. In this study, we investigated the association of Lin28 with paclitaxel resistance and identified the underlying mechanisms of action of Lin28 in human breast cancer cell lines and tumor tissues. We found that the expression level of Lin28 was closely associated with the resistance to paclitaxel treatment. The T47D cancer cell line, which highly expresses Lin28, is more resistant to paclitaxel than the MCF7, Bcap-37 or SK-BR-3 cancer cell lines, which had low-level expression of Lin28. Knocking down of Lin28 in Lin28 high expression T47D cells increased the sensitivity to paclitaxel treatment, while stable expression of Lin28 in breast cancer cells effectively attenuated the sensitivity to paclitaxel treatment, resulting in a significant increase of IC50 values of paclitaxel. Transfection with Lin28 also significantly inhibited paclitaxel-induced apoptosis. We also found that Lin28 expression was dramatically increased in tumor tissues after neoadjuvant chemotherapy or in local relapse or metastatic breast cancer tissues. Moreover, further studies showed that p21, Rb and Let-7 miRNA were the molecular targets of Lin28. Overexpression of Lin28 in breast cancer cells considerably induced p21 and Rb expression and inhibited Let-7 miRNA levels. Our results indicate that Lin28 expression might be one mechanism underlying paclitaxel resistance in breast cancer, and Lin28 could be a potential target for overcoming paclitaxel resistance in breast cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0040008PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3392258PMC
March 2013

Effect of dietary intake of isoflavones on the estrogen and progesterone receptor status of breast cancer.

Nutr Cancer 2010 ;62(6):765-73

School of Population Health, The University of Western Australia, 35 Stirling Highway, Crawley, Perth, Western Australia 6009, Australia.

To examine if higher intake of isoflavones prior to diagnosis was associated with a positive status of estrogen receptors (ER) and progesterone receptors (PR) in breast tumor tissue, a retrospective study was conducted in 2004 to 2005 in 756 Chinese women with histologically confirmed breast cancer. We administered a food frequency questionnaire by face-to-face interview to assess the intake of the isoflavones daidzein and genistein. Unconditional logistic regression analysis was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) adjusted for potential confounders. Comparing the highest to lowest intake quartiles, the adjusted ORs for daidzein were 1.94 (95% CI = 1.20-3.32) and 2.18 (95% CI = 1.28-3.73) for ER positive and PR positive tumors, respectively, in premenopausal patients. The ORs for both ER and PR positive status combined were 2.48 (95% CI = 1.37-4.49) for daidzein and 1.94 (95% CI = 1.21-3.14) for genistein in premenopausal patients with statistically significant tests for trend. There was little or no evidence of associations in postmenopausal patients. We conclude that higher intake of daidzein and genistein before diagnosis was associated with ER and PR positive status in premenopausal Chinese women with breast cancer. The association might confer a more favorable prognosis.
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http://dx.doi.org/10.1080/01635581003605979DOI Listing
November 2010

Synthesis, spectroscopy and photochemistry of nitro-azobenzene dyes bearing benzophenone parts.

J Fluoresc 2009 May 19;19(3):533-44. Epub 2008 Nov 19.

College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China.

Novel nitro-azobenzene dyes bearing one or two benzophenone branches were proposed and synthesized to improve their photophysical and photochemical properties. The new dyes exhibited double UV/visible bands, and they displayed weak fluorescence emission as excited at 350 nm. Single crystal X-ray diffraction data showed that two phenyl rings of azobenzene was almost coplanar, and the benzophenone part was neither coplanar nor linear connection with azobenzene via ether bridged bond, which have good fit with molecular geometry optimization calculation results. The cyclic voltammeric results of nitro-azobenzene dyes were firstly reported in this paper, which demonstrated that the electrochemical properties of nitro-azobenzene dyes was altered by the substitution of benzophenone part. Thermal stabilities of the new dyes were studied by the analysis of differential scanning calorimetry (DSC) and thermograving (TG) in this paper. Efficient visible-light photoinitiating polymerization of methyl methacrylate (MMA) by the novel nitro-azobenzene dyes was presented and discussed.
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http://dx.doi.org/10.1007/s10895-008-0442-yDOI Listing
May 2009

Study of the absorption and emission spectroscopy of "A-B" type photosensitive compounds including two-photon chromophore and benzophenone moiety.

Spectrochim Acta A Mol Biomol Spectrosc 2008 Oct 22;70(5):1006-12. Epub 2007 Oct 22.

College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China.

"A-B" type photosensitive compounds including two-photon chromophore and benzophenone moiety have been designed, synthesized and characterized. The UV-vis absorption and fluorescence emission of the compounds have been extensively studied in various solvents. The results show that the absorption of "A-B" type compounds displays obvious double absorption bands, one of which at short-wavelength is related to the benzophenone moiety, the other at long-wavelength is mainly contributed by chromophore. The emission of "A-B" type compounds at 500-700 nm shows an "unexpected" blue-shift comparing with that of the sole chromophore. The photosensitive compounds with amino group display strong emission in apolar solvents and have a low fluorescence quantum yields in polar solvents. In contrast, the compounds without amino group exhibit strong fluorescence emission in polar solvents, and low fluorescence quantum yields in apolar solvents. The fluorescence quantum yields of "A-B" type compounds are higher than those of the sole chromophore. The discoveries suggest that charge redistribution induced by the introduction of benzophenone moiety plays a key role on the absorption and emission spectroscopy.
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http://dx.doi.org/10.1016/j.saa.2007.07.064DOI Listing
October 2008