Publications by authors named "Xiaohui Wu"

297 Publications

Synergistic effect of floatable hydroxyapatite-modified biochar adsorption and low-level CaCl leaching on Cd removal from paddy soil.

Sci Total Environ 2021 Oct 7:150872. Epub 2021 Oct 7.

Brook Byers Institute for Sustainable Systems, School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States.

The utilization of recycled biochar combined with chemical leaching is an appropriate method to remove cadmium (Cd) from paddy soil. Some Cd-rich soil clay particulates (particulate Cd) are reported to be removed via biochar adsorption and the potential impact of biochar on soil properties need further study. The removal efficiencies and mechanisms of Cd from soil by using floatable hydroxyapatite modified biochar (HBC) combined with CaCl were studied. The synergetic removal efficiencies of total Cd (46.5%) and bioavailable Cd (37.9%) from the paddy soil were achieved with 2% HBC and 1 mM CaCl. The increased soluble Cd in soil pore water by CaCl leaching could be efficiently adsorbed on HBC, and removed by HBC collection, reducing the risk of the residual soluble Cd in soil pore water to rice plants caused by the inefficient drainage in the field. The suspendability of clay particulates in overlying water was little affected by the low-level CaCl based on Derjaguin-Landau-Verwey-Overbeek (DLVO) calculation. Moreover, low-level CaCl facilitated the accumulation of particulate Cd on the floating HBC via decreasing the interaction energy (by 25%) between clay particulates and HBC. HBC-mediated Cd migration contributed ~70% of total Cd removal, while soluble and particulate Cd removed through the drainage accounted for ~30%. Soil clay proportion maintained at 25.3% due to the replenishment of HBC residues. In addition, soil nutrient and physicochemical conditions were improved with HBC residues. This work provides a novel soil remediation method by using floatable biochar combined with low-level CaCl for Cd-contaminated paddy soil remediation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150872DOI Listing
October 2021

Dietary patterns and sarcopenia in elderly adults: the TCLSIH study.

Br J Nutr 2021 Sep 27:1-26. Epub 2021 Sep 27.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Sarcopenia is a core contributor to several health consequences, including falls, fractures, physical limitations, and disability. The pathophysiological processes of sarcopenia may be counteracted with the proper diet, delaying sarcopenia onset. Dietary pattern analysis is a whole diet approach used to investigate the relationship between diet and sarcopenia. Here we aimed to investigate this relationship in an elderly Chinese population. A cross-sectional study with 2,423 participants aged more than 60 years was performed. Sarcopenia was defined based on the guidelines of the Asian Working Group for Sarcopenia, composed of low muscle mass plus low grip strength and/or low gait speed. Dietary data were collected using a food-frequency questionnaire that included questions on 100 food items along with their specified serving sizes. Three dietary patterns were derived by factor analysis: sweet pattern; vegetable pattern; animal food pattern. The prevalence of sarcopenia was 16.1%. The higher vegetable pattern score and animal food pattern score were related to lower prevalence of sarcopenia (Ptrend =0.006 and Ptrend <0.001, respectively); the multivariate-adjusted odds ratio (95% confidence interval) of the prevalence of sarcopenia in the highest versus lowest quartiles were 0.54 (0.34, 0.86) and 0.50 (0.33, 0.74), separately. The sweet pattern score was not significantly related to the prevalence of sarcopenia. The present study showed that vegetable pattern and animal food pattern were related to a lower prevalence of sarcopenia in Chinese older adults. Further studies are required to clarify these findings.
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http://dx.doi.org/10.1017/S0007114521003871DOI Listing
September 2021

The effect of thyroid function on the risk of psychiatric readmission after hospitalization for major depressive disorder.

Psychiatry Res 2021 Sep 6;305:114205. Epub 2021 Sep 6.

Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 200031, China; Shanghai Key Laboratory of Psychotic Disorders, Shanghai 201108, China; Guangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired Intelligence, Guangzhou 510515, China. Electronic address:

The relationship between thyroid function and depression has long been recognized, but little is known about the effect of thyroid function on the risk of readmission after hospitalization for major depressive disorder (MDD). This retrospective cohort study was aimed to explore the effect of thyroid function on psychiatric readmission after hospitalization for MDD. Data was derived from electronic medical records (EMR) of the Shanghai Mental Health Center (SMHC), Shanghai, China. Univariate and multivariate logistic regression analyses were conducted in subjects aged ≥ 18 years who had been hospitalized for MDD between January 1, 2007, and May 31, 2019. Of the 1803 eligible patients, 85 and 132 patients experienced psychiatric readmission within 90 days and 180 days after discharge respectively. Multivariate analyses showed that serum FT3 level (aOR=1.271; 95%CI=1.051-1.537) and comorbidity of thyroid disease (aOR=2,179; 95%CI=1.136-4.179) was independently associated with the risk of 90-day and 180-day readmission respectively. These findings indicated that high serum FT3 levels and comorbidity of thyroid disease could increase the risk of readmission after hospitalization for MDD. It is warranted to provide routine assessment and intervention of the thyroid function during the treatment of depression so as to prevent re-hospitalization.
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http://dx.doi.org/10.1016/j.psychres.2021.114205DOI Listing
September 2021

Quercetin reverses chronic unpredictable mild stress-induced depression-like behavior in vivo by involving nuclear factor-E2-related factor 2.

Brain Res 2021 Sep 13;1772:147661. Epub 2021 Sep 13.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.

Quercetin is a flavonoid compound rich in many natural plants with a wide range of pharmacological effects and nutritional value. Although previous studies have initially shown the antidepressant effect of quercetin in some models. However, the exact mechanism of the antidepressant effect of quercetin on the depression model induced by chronic unpredictable mild stress (CUMS) is still unclear or has not been clearly elucidated. The present study aimed to investigate the antidepressant effect of quercetin in vivo on a CUMS-induced depression model that is closest to human depression, and to explore its mechanism of action around nuclear factor-E2-related factor 2 (Nrf2) related signaling pathways, for the first time. Our results demonstrated that CUMS for 21 consecutive days caused significant decreases in the sucrose preference, and the horizontal score and vertical score in the open field test of mice respectively by 22.6%, 34.4%, and 66.6% (all P < 0.01), and a significant increase in the immobility time during the forced swimming test by 110.5% (P < 0.01), but fortunately, after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 26.2%, 40.1%, 152.7%, 43.5% (all P < 0.01). Further western blot analysis showed that CUMS caused the phosphorylation or expression levels of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), Nrf2 and heme oxygenase-1 (HO-1) proteins in the hippocampus of mice to significantly down-regulate by 60.0%, 72.1%, 90.0% and 50.1% (all P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of these proteins were significantly up-regulated by 85.8%, 182.0%, 325.1% and 60.3% (all P < 0.01). In addition, CUMS also caused significant reduction in the levels of antioxidants including superoxide dismutase (SOD) and glutathione-s transferase (GST) in the mice hippocampus by 51.3%, 40.3% (both P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 69.2% and 49.5% (both P < 0.01), as well as significant elevation in the levels of lipid peroxide malondialdehyde (MDA), inflammation medium nitric oxide (NO) and inducible nitric oxide synthase (iNOS) by 156.4%, 255.4% and 72.7% (all P < 0.01), while after chronic oral administration of high dose quercetin at 40 mg/kg, the abnormalities of the above indicators were significantly reversed by 45.9%, 26.8% and 55.2% (all P < 0.01). The medium dose of quercetin (20 mg/kg) only reversed some of the above indicators, while the low dose of quercetin (10 mg/kg) had no reversal effect on the above indicators. Collectively, the present study confirmed for the first time that quercetin weakened CUMS-induced depression in vivo, and its mechanism was at least partially attributable to the upregulation of hippocampal Nrf2 and the inhibition of iNOS, thereby correcting the central inflammatory response, and the imbalance between oxidation and antioxidant.
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http://dx.doi.org/10.1016/j.brainres.2021.147661DOI Listing
September 2021

Peptidomic Analysis of Neonate Umbilical Cord Blood for the Identification of Endogenous Peptides Involved in Hypoxic-Ischemic Encephalopathy.

Front Pediatr 2021 25;9:718704. Epub 2021 Aug 25.

Department of Pediatric, Jingjiang People's Hospital Affiliated to Yangzhou University, Jingjiang, China.

Neonatal hypoxic-ischemic encephalopathy (HIE) is a common neurological disorder triggered by perinatal cerebral ischemia and hypoxia. Accumulating evidence has shown that peptides have neuroprotective effects in nerve injury. However, the function of endogenous peptides in the pathogenesis of HIE has not been studied. In the present study, a comparative peptidomic profile was performed in the serum of the human umbilical cord blood with HIE (three patients) and the control group (three health control) by liquid chromatography-mass spectrometry (LC-MS). Our study demonstrated that a total of 49 peptides derived from 25 precursor proteins were differentially expressed in the serum of HIE compared with normal controls, including 33 upregulated peptides and 16 downregulated peptides. Each of the differentially expressed peptides has specific characteristics, including pI, Mw, and cleavage pattern. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the precursor proteins of differentially expressed peptides participate in the different biological process. Moreover, among the 49 differentially expressed peptides, 21 peptides were identified from the fibrinogen chain family, which plays a role in neurological diseases, suggesting that these peptides may play an important role in maintaining brain health. In conclusion, our results showed a comparative peptidomic profile from human umbilical cord blood of HIE patients and normal controls. These dysregulated peptides may have potentially important functions in umbilical cord blood with HIE and may be involved in the pathogenesis of the HIE.
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http://dx.doi.org/10.3389/fped.2021.718704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424085PMC
August 2021

scAPAdb: a comprehensive database of alternative polyadenylation at single-cell resolution.

Nucleic Acids Res 2021 Sep 11. Epub 2021 Sep 11.

Pasteurien College, Soochow University, Suzhou, Jiangsu 215000, China.

Alternative polyadenylation (APA) is a widespread regulatory mechanism of transcript diversification in eukaryotes, which is increasingly recognized as an important layer for eukaryotic gene expression. Recent studies based on single-cell RNA-seq (scRNA-seq) have revealed cell-to-cell heterogeneity in APA usage and APA dynamics across different cell types in various tissues, biological processes and diseases. However, currently available APA databases were all collected from bulk 3'-seq and/or RNA-seq data, and no existing database has provided APA information at single-cell resolution. Here, we present a user-friendly database called scAPAdb (http://www.bmibig.cn/scAPAdb), which provides a comprehensive and manually curated atlas of poly(A) sites, APA events and poly(A) signals at the single-cell level. Currently, scAPAdb collects APA information from > 360 scRNA-seq experiments, covering six species including human, mouse and several other plant species. scAPAdb also provides batch download of data, and users can query the database through a variety of keywords such as gene identifier, gene function and accession number. scAPAdb would be a valuable and extendable resource for the study of cell-to-cell heterogeneity in APA isoform usages and APA-mediated gene regulation at the single-cell level under diverse cell types, tissues and species.
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http://dx.doi.org/10.1093/nar/gkab795DOI Listing
September 2021

Antidepressant effect of catalpol on corticosterone-induced depressive-like behavior involves the inhibition of HPA axis hyperactivity, central inflammation and oxidative damage probably via dual regulation of NF-κB and Nrf2.

Brain Res Bull 2021 Sep 6;177:81-91. Epub 2021 Sep 6.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.

This study aimed to investigate the antidepressant effect and mechanism of catalpol on corticosterone (CORT)-induced depressive-like behavior in mice for the first time. As a result, CORT injection induced depressive-like behaviors of mice in behavioral tests, aggravated the serum CORT, adrenocorticotropic hormone, and corticotropin-releasing hormone levels, and conspicuously elevated the phosphorylations of nuclear factor kappa-B (NF-κB) in the hippocampus and frontal cortex, and down-regulated the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2). Furthermore, CORT exposure dramatically augmented the levels of inflammatory factors (interleukin-1β, tumor necrosis factor-α, nitric oxide synthase, and nitric oxide) and lipid peroxidation product malondialdehyde, and attenuated the levels of antioxidants including reduced glutathione, glutathione S-transferase, total superoxide dismutase, and heme oxygenase-1 in the mouse hippocampus and frontal cortex. On the contrary, catalpol administration markedly suppressed the abnormalities of the above indicators. From the overall results, this study displayed that catalpol exerted a beneficial effect on CORT-induced depressive-like behavior in mice possibly via the inhibition of hypothalamus-pituitary-adrenal (HPA) axis hyperactivity, central inflammation and oxidative damage at least partially through dual regulation of NF-κB and Nrf2.
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http://dx.doi.org/10.1016/j.brainresbull.2021.09.002DOI Listing
September 2021

scLINE: A multi-network integration framework based on network embedding for representation of single-cell RNA-seq data.

J Biomed Inform 2021 Oct 3;122:103899. Epub 2021 Sep 3.

Department of Automation, Xiamen University, China. Electronic address:

Single-cell RNA sequencing (scRNA-seq) is fast becoming a powerful technology that revolutionizes biomedical studies related to development, immunology and cancer by providing genome-scale transcriptional profiles at unprecedented throughput and resolution. However, due to the low capture rate and frequent drop-out events in the sequencing process, scRNA-seq data suffer from extremely high sparsity and variability, challenging the data analysis. Here we proposed a novel method called scLINE for learning low dimensional representations of scRNA-seq data. scLINE is based on the network embedding model that jointly considers multiple gene-gene interaction networks, facilitating the incorporation of prior biological knowledge for signal extraction. We comprehensively evaluated scLINE on eight single-cell datasets. Results show that scLINE achieved comparable or higher performance than competing methods, including PCA, t-SNE and Isomap, in terms of internal validation metrics and clustering accuracy. The low dimensional representations learned by scLINE are effective for downstream single-cell analysis, such as visualization, clustering and cell typing. We have implemented scLINE as an easy-to-use R package, which can be incorporated in other existing scRNA-seq analysis pipelines or tools for data preprocessing.
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http://dx.doi.org/10.1016/j.jbi.2021.103899DOI Listing
October 2021

An RNA/DNA hybrid origami-based nanoplatform for efficient gene therapy.

Nanoscale 2021 Aug 22;13(30):12848-12853. Epub 2021 Jul 22.

CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, 11 BeiYiTiao, ZhongGuanCun, Beijing 100190, China.

Nucleic acid nanostructures are promising biomaterials for the delivery of homologous gene therapy drugs. Herein, we report a facile strategy for the construction of target mRNA (scaffold) and antisense (staple strands) co-assembled RNA/DNA hybrid "origami" for efficient gene therapy. In our design, the mRNA was folded into a chemically well-defined nanostructure through RNA-DNA hybridization with high yield. After the incorporation of an active cell-targeting aptamer, the tailored RNA/DNA hybrid origami demonstrated efficient cellular uptake and controllable release of antisenses in response to intracellular RNase H digestion. The biocompatible RNA/DNA origami (RDO) elicited a noticeable inhibition of cell proliferation based on the silencing of the tumor-associated gene polo-like kinase 1 (PLK1). This RDO-based nanoplatform provides a novel strategy for the further development of gene therapy.
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http://dx.doi.org/10.1039/d1nr00517kDOI Listing
August 2021

Revealing the heterogeneous activation mechanism of peroxydisulfate by CuO: the critical role of surface-binding organic substrates.

Sci Total Environ 2021 Aug 24;802:149833. Epub 2021 Aug 24.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Environmental Science and Engineering, University of Science and Technology of China, Hefei 230026, China. Electronic address:

Heterogeneous catalytic activation mechanisms of peroxydisulfate (PDS) by transition metal oxides are generally attributed to the interactions between catalysts and PDS, however, the role of the co-existed organic substrate was largely overlooked in the past studies. In this work, phenol was selected as the target organic pollutant in a CuO/PDS system to evaluate its deep-seated role in participating in the effective activation of PDS. First, optimized reaction conditions as pH of 6.0, CuO of 5.96 g·L and PDS of 2.5 mM were obtained by the response surface methodology (RSM) with a phenol degradation efficiency of 84.0%. It was further found that pre-adsorption of phenol or PDS led to obviously different performances in the phenol degradation with/without the radical scavengers. Two different activation pathways of PDS, i.e., the non-radical pathway mediated by surface deprotonated phenol to generate O and the radical pathway mediated by structural Cu(I)/Cu(II) to produce SO, were therefore proposed, and the former was predominant in the CuO/PDS/phenol system. In addition, HCO and HPO could strongly inhibit the phenol degradation while Cl and NO only performed negligible effects. NaOH washing could regenerate the surface hydroxyl groups and recover the catalytic ability of CuO. The result of this study integrated the interactions among the catalyst, oxidant and substrate, providing new insights into environmental-friendly PDS activation processes.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149833DOI Listing
August 2021

Peripheral biomarkers to predict the diagnosis of bipolar disorder from major depressive disorder in adolescents.

Eur Arch Psychiatry Clin Neurosci 2021 Aug 25. Epub 2021 Aug 25.

Clinical Research Center and Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.

The onset of bipolar disorder (BD) occurs in childhood or adolescence in half of the patients. Early stages of BD usually present depressive episodes, which makes it difficult to be distinguished from major depressive disorder (MDD). Objective biomarkers for discriminating BD from MDD in adolescent patients are limited. We collected basic demographic data and the information of the first blood examination performed after the admission to psychiatry unit of BD and MDD inpatients during 2009-2018. We recruited 261 adolescents (aged from 10 to 18), including 160 MDD and 101 BD. Forward-Stepwise Selection of binary logistic regression was used to construct predictive models for the total sample and subgroups by gender. Independent external validation was made by 255 matched patients from another hospital in China. Regression models of total adolescents, male and female subgroups showed accuracy of 73.3%, 70.6% and 75.2%, with area under curves (AUC) as 0.785, 0.816 and 0.793, respectively. Age, direct bilirubin (DBIL), lactic dehydrogenase (LDH), free triiodothyronine (FT3) and C-reactive protein (CRP) were final factors included into the models. The discrimination was well at external validation (AUC = 0.714). This study offers the evidence that accessible information of common clinical laboratory examination might be valuable in distinguishing BD form MDD in adolescents. With good diagnostic accuracies and external validation, the total regression equation might potentially be applied to individualized clinical inferences on adolescent BD patients.
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http://dx.doi.org/10.1007/s00406-021-01321-4DOI Listing
August 2021

Peripheral biomarkers to predict the diagnosis of bipolar disorder from major depressive disorder in adolescents.

Eur Arch Psychiatry Clin Neurosci 2021 Aug 25. Epub 2021 Aug 25.

Clinical Research Center and Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.

The onset of bipolar disorder (BD) occurs in childhood or adolescence in half of the patients. Early stages of BD usually present depressive episodes, which makes it difficult to be distinguished from major depressive disorder (MDD). Objective biomarkers for discriminating BD from MDD in adolescent patients are limited. We collected basic demographic data and the information of the first blood examination performed after the admission to psychiatry unit of BD and MDD inpatients during 2009-2018. We recruited 261 adolescents (aged from 10 to 18), including 160 MDD and 101 BD. Forward-Stepwise Selection of binary logistic regression was used to construct predictive models for the total sample and subgroups by gender. Independent external validation was made by 255 matched patients from another hospital in China. Regression models of total adolescents, male and female subgroups showed accuracy of 73.3%, 70.6% and 75.2%, with area under curves (AUC) as 0.785, 0.816 and 0.793, respectively. Age, direct bilirubin (DBIL), lactic dehydrogenase (LDH), free triiodothyronine (FT3) and C-reactive protein (CRP) were final factors included into the models. The discrimination was well at external validation (AUC = 0.714). This study offers the evidence that accessible information of common clinical laboratory examination might be valuable in distinguishing BD form MDD in adolescents. With good diagnostic accuracies and external validation, the total regression equation might potentially be applied to individualized clinical inferences on adolescent BD patients.
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http://dx.doi.org/10.1007/s00406-021-01321-4DOI Listing
August 2021

Antidepressant mechanism of catalpol: Involvement of the PI3K/Akt/Nrf2/HO-1 signaling pathway in rat hippocampus.

Eur J Pharmacol 2021 Oct 29;909:174396. Epub 2021 Jul 29.

College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.

Catalpol is a major compound in Rehmanniae Radix with outstanding medicinal and nutritional values. Our previous studies have demonstrated catalpol's antidepressant effect, but its mechanisms remain unclear. This study aimed to explore the antidepressant mechanisms of catalpol via the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1 (HO-1) pathway. Results demonstrated that chronic unpredictable mild stress (CUMS) for 5 consecutive weeks caused significant decreases in the sucrose preference and the horizontal and vertical scores of open-field test, as well as a significant increase in the swimming-immobility time of rats; catalpol administration significantly reversed the abnormality of these indicators. Further real-time fluorescent quantitative polymerase chain reaction and Western blotting results together showed that CUMS significantly downregulated the expression levels of hippocampal genes and proteins, including PI3K, Akt, Nrf2, HO-1, tropomyosin-related kinase B (TrkB), and brain-derived neurotrophic factor; catalpol administration significantly reversed the abnormal expression of these genes and proteins. CUMS also caused a significant decrease in the hippocampal superoxide dismutase, catalase, glutathione peroxidase, glutathione-s transferase, and reduced glutathione levels, as well as a significant increase in thiobarbituric acid reactive substances level in rats; catalpol administration significantly reversed the abnormality of these indicators. Taken together, this study confirmed for the first time that the antidepressant effect of catalpol on CUMS-induced depression involved the upregulation of the PI3K/Akt/Nrf2/HO-1 signaling pathway, thereby improving the hippocampal neurotrophic, neuroprotective, and antioxidant levels. The PI3K/Akt/Nrf2/HO-1 pathway-related molecules may serve as potential new biomarkers and candidate molecular targets for catalpol's antidepressant effects.
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http://dx.doi.org/10.1016/j.ejphar.2021.174396DOI Listing
October 2021

The chemerin-CMKLR1 axis limits thermogenesis by controlling a beige adipocyte/IL-33/type 2 innate immunity circuit.

Sci Immunol 2021 Jul;6(61)

Department of Immunology and Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.

IL-33-associated type 2 innate immunity has been shown to support beige fat formation and thermogenesis in subcutaneous inguinal white adipose tissue (iWAT), but little is known about how it is regulated in iWAT. Chemerin, as a newly identified adipokine, is clinically associated with obesity and metabolic disorders. We here show that cold exposure specifically reduces chemerin and its receptor chemerin chemokine-like receptor 1 (CMKLR1) expression in iWAT. Lack of chemerin or adipocytic CMKLR1 enhances cold-induced thermogenic beige fat via potentiating type 2 innate immune responses. Mechanistically, we identify adipocytes, particularly beige adipocytes, as the main source for cold-induced IL-33, which is restricted by the chemerin-CMKLR1 axis via dampening cAMP-PKA signaling, thereby interrupting a feed-forward circuit between beige adipocytes and type 2 innate immunity that is required for cold-induced beige fat and thermogenesis. Moreover, specific deletion of adipocytic IL-33 inhibits cold-induced beige fat and type 2 innate immune responses. Last, genetic blockade of adipocytic CMKLR1 protects against diet-induced obesity and enhances the metabolic benefits of cold stimulation in preestablished obese mice. Thus, our study identifies the chemerin-CMKLR1 axis as a physiological negative regulator of thermogenic beige fat via interrupting adipose-immune communication and suggests targeting adipose CMKLR1 as a potential therapeutic strategy for obesity-related metabolic disorders.
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http://dx.doi.org/10.1126/sciimmunol.abg9698DOI Listing
July 2021

[Clinical analysis of treatment and postoperative efficacy in neonatal congenital pyriform sinus fistula].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 May;35(5):444-448

Department of Otolaryngology Head and Neck Surgery,Xiamen Children's Hospital,Xiamen,361006,China.

To discuss the diagnosis and treatment of congenital pyriform sinus fistula(CPSF) in newborn. Clinical data of 5 patients with CPSF innewborn were reviewed and the clinical symptoms, auxiliary examinations, surgical methods were analyzed after the operation, patients were followed up closely at different stages. All the 5 neonates successfully completed the surgery without pharyngeal fistula, dysphagia, perifistula and distal fistula infection. Follow-up survey ranged from 3 months to 2 years and no one recurred. Neonatal CPSF is a rare disease with a short course of disease and rapid progression. In severe cases, it may threaten life and should be treated in time.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.05.014DOI Listing
May 2021

Transcriptional regulation of N-methyladenosine orchestrates sex-dimorphic metabolic traits.

Nat Metab 2021 07 19;3(7):940-953. Epub 2021 Jul 19.

Division of Cardiology, Department of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.

Males and females exhibit striking differences in the prevalence of metabolic traits including hepatic steatosis, a key driver of cardiometabolic morbidity and mortality. RNA methylation is a widespread regulatory mechanism of transcript turnover. Here, we show that presence of the RNA modification N-methyladenosine (mA) triages lipogenic transcripts for degradation and guards against hepatic triglyceride accumulation. In male but not female mice, this protective checkpoint stalls under lipid-rich conditions. Loss of mA control in male livers increases hepatic triglyceride stores, leading to a more 'feminized' hepatic lipid composition. Crucially, liver-specific deletion of the mA complex protein Mettl14 from male and female mice significantly diminishes sex-specific differences in steatosis. We further surmise that the mA installing machinery is subject to transcriptional control by the sex-responsive BCL6-STAT5 axis in response to dietary conditions. These data show that mA is essential for precise and synchronized control of lipogenic enzyme activity and provide insights into the molecular basis for the existence of sex-specific differences in hepatic lipid traits.
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http://dx.doi.org/10.1038/s42255-021-00427-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422857PMC
July 2021

QuantifyPoly(A): reshaping alternative polyadenylation landscapes of eukaryotes with weighted density peak clustering.

Brief Bioinform 2021 Jul 13. Epub 2021 Jul 13.

Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361102, China.

The dynamic choice of different polyadenylation sites in a gene is referred to as alternative polyadenylation, which functions in many important biological processes. Large-scale messenger RNA 3' end sequencing has revealed that cleavage sites for polyadenylation are presented with microheterogeneity. To date, the conventional determination of polyadenylation site clusters is subjective and arbitrary, leading to inaccurate annotations. Here, we present a weighted density peak clustering method, QuantifyPoly(A), to accurately quantify genome-wide polyadenylation choices. Applying QuantifyPoly(A) on published 3' end sequencing datasets from both animals and plants, their polyadenylation profiles are reshaped into myriads of novel polyadenylation site clusters. Most of these novel polyadenylation site clusters show significantly dynamic usage across different biological samples or associate with binding sites of trans-acting factors. Upstream sequences of these clusters are enriched with polyadenylation signals UGUA, UAAA and/or AAUAAA in a species-dependent manner. Polyadenylation site clusters also exhibit species specificity, while plants ones generally show higher microheterogeneity than that of animals. QuantifyPoly(A) is broadly applicable to any types of 3' end sequencing data and species for accurate quantification and construction of the complex and dynamic polyadenylation landscape and enables us to decode alternative polyadenylation events invisible to conventional methods at a much higher resolution.
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http://dx.doi.org/10.1093/bib/bbab268DOI Listing
July 2021

Integrating sulfur, iron(II), and fixed organic carbon for mixotrophic denitrification in a composite filter bed reactor for decentralized wastewater treatment: Performance and microbial community.

Sci Total Environ 2021 Nov 3;795:148825. Epub 2021 Jul 3.

School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

Decentralized wastewater treatment in rural areas is an imperative challenge around the world, particularly in developing countries. The composite filter bed reactor is viable for decentralized wastewater treatment, but its performance on nitrogen removal often fluctuates with the unstable influent characteristics and loadings. Here, a composite filter bed reactor integrating sulfur, iron(II), and fixed organic carbon (shaddock peel) was developed and continuously operated under different conditions. The fixed organic carbon source promoted nitrogen removal with an efficiency higher than 90% and reduced effluent sulfate level by 40%, indicating that the integrated electron donors could improve the resistance and stability of the reactor. Moreover, sulfur-oxidizing bacteria (Thiomonas, Sulfuriferula, and Acidithiobacillus), iron-oxidizing bacteria (Ferritrophicum), and denitrifiers (Simplicispira and Hydrogenophaga) were identified in the anoxic/anaerobic layer of the reactor, suggesting that mixotrophic denitrification was stimulated by sulfur, iron(II), and fixed organic carbon. The findings of this study indicate that the developed reactor with the integrated electron donors could be reliable for carbon, nitrogen, and phosphorus removal and promising for the application of decentralized wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148825DOI Listing
November 2021

GCSF As a Potential Molecular Target for Overall Survival of Hepatocellular Carcinoma.

Comb Chem High Throughput Screen 2021 Mar 22. Epub 2021 Mar 22.

Department of Hepatobiliary Surgery, The Fourth Hospital of Hebei Medical University, 12 Health Road, Shijiazhuang, Hebei 050011, China.

Background: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors of digestive tract in the world. Therefore, it is important to carry out studies on the molecular mechanisms of early diagnosis and treatment of HCC to reduce mortality.

Methods: Bioinformatic analysis was performed to explore the significant role of GCSF on the occurrence and development of neoplasm. Differently expressed genes (DEGs) were screened, and the significant hub genes related with GCSF were identified by the multiple algorithms of Cytoscape. Functional annotation for DEGs, pathological stage and overall survival analysis were implemented. In addition, the verification for the role of GCSF on HCC was made via the clinical samples. A total of 70 participates diagnosed as HCC were recruited from November 2014 to November 2019. The immunohistochemistry assay, qRT-PCR, receiver operating characteristic (ROC) curves, and overall survival analysis were carried out.

Results: GCSF was related with the tumor size, and the expression of GCSF was up-regulated in hepatocellular carcinoma tissues. The enrichment results of GO and KEGG analysis were mainly enriched in "Inflammatory response", "Protein binding", "Metabolic pathways", and "Proteasome". The tumor diameter (P < 0.001), and survival time (P < 0.001) were significantly associated with expression of GCSF via the verification of clinical data. The univariate and multivariate Cox proportional regression analysis manifested that high expression of GCSF in patients with HCC was related to poor OS.

Conclusion: The expression level of GCSF is significantly associated with the prognostic survival of HCC, and it is expected to become a new prognostic marker of HCC, providing a novel idea for future basic research as well as targeted therapy.
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http://dx.doi.org/10.2174/1386207324666210322124003DOI Listing
March 2021

Abnormal Fibrinogen Level as a Prognostic Indicator in Coronavirus Disease Patients: A Retrospective Cohort Study.

Front Med (Lausanne) 2021 14;8:687220. Epub 2021 Jun 14.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

The coronavirus disease (COVID-19) pandemic poses a global threat, and identification of its prognostic biomarkers could prove invaluable. Fibrinogen (FIB) could be one such indicator as coagulation and fibrinolysis abnormalities are common among COVID-19 patients. We examined the role of FIB levels in the prognosis of COVID-19. This retrospective cohort study enrolled 1,643 COVID-19 patients from the Leishenshan Hospital in Wuhan, China. The follow-up was conducted from February 8, 2020 to April 15, 2020. The cohort was divided into three groups according to the FIB level on admission, and associations with mortality and disease severity were determined using Cox and logistic regression analyses, respectively. Further, Kaplan-Meier (K-M) analyses by log-rank tests were used to assess the survival of patients with varying FIB levels. Patients with FIB < 2.2 g/L [hazard ratio (HR): 9.02, 95% confidence interval (CI): 1.91-42.59, = 0.006] and >4.2 g/L (HR: 4.79, 95% CI: 1.14-20.20, = 0.033) showed higher mortality risks compared to those with FIB between 2.2 and 4.2 g/L. The survival curves showed similar results in K-M analyses ( < 0.001). Additionally, an elevated FIB level was associated with a greater risk of developing critical disease (odds ratio: 2.16, 95% CI: 1.04-4.46, = 0.038) than a FIB level within the normal range. Abnormal FIB levels may be associated with mortality risk among COVID-19 patients and could predict critical disease development. Thus, assessment of FIB levels may assist in determining the prognosis of COVID-19 patients.
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http://dx.doi.org/10.3389/fmed.2021.687220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236504PMC
June 2021

Injectable and thermosensitive hydrogels mediating a universal macromolecular contrast agent with radiopacity for noninvasive imaging of deep tissues.

Bioact Mater 2021 Dec 23;6(12):4717-4728. Epub 2021 May 23.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Shanghai Stomatological Hospital, Fudan University, Shanghai, 200438, China.

It is very challenging to visualize implantable medical devices made of biodegradable polymers in deep tissues. Herein, we designed a novel macromolecular contrast agent with ultrahigh radiopacity (iodinate content > 50%) via polymerizing an iodinated trimethylene carbonate monomer into the two ends of poly(ethylene glycol) (PEG). A set of thermosensitive and biodegradable polyester-PEG-polyester triblock copolymers with varied polyester compositions synthesized by us, which were soluble in water at room temperature and could spontaneously form hydrogels at body temperature, were selected as the demonstration materials. The addition of macromolecular contrast agent did not obviously compromise the injectability and thermogelation properties of polymeric hydrogels, but conferred them with excellent X-ray opacity, enabling visualization of the hydrogels at clinically relevant depths through X-ray fluoroscopy or Micro-CT. In a mouse model, the 3D morphology of the radiopaque hydrogels after injection into different target sites was visible using Micro-CT imaging, and their injection volume could be accurately obtained. Furthermore, the subcutaneous degradation process of a radiopaque hydrogel could be non-invasively monitored in a real-time and quantitative manner. In particular, the corrected degradation curve based on Micro-CT imaging well matched with the degradation profile of virgin polymer hydrogel determined by the gravimetric method. These findings indicate that the macromolecular contrast agent has good universality for the construction of various radiopaque polymer hydrogels, and can nondestructively trace and quantify their degradation . Meanwhile, the present methodology developed by us affords a platform technology for deep tissue imaging of polymeric materials.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8165329PMC
December 2021

Numerical investigation on the transmission and dispersion of aerosols in a 7-stories building drainage system.

Build Environ 2021 Aug 28;201:108009. Epub 2021 May 28.

School of Environment Science and Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China.

In previous reports, the positive SARS-CoV-2 nucleic acid was detected in the fecal samples from confirmed pneumonia patients, suggesting a high probability of the fecal-oral transmission. To date, however, the role played by the drainage system of a high-rise building in the virus transmission is not clear and especially studies on the dynamics mechanism behind is scarce. From this point of view, the present work carries out a computational fluid dynamics (CFD) modeling to investigate the effects of the water seal effectiveness of the floor drain, the negative/positive pressures ( , ) in the bathroom, temperature differential (), outside wind velocity (), the piping fittings and the negative pressure at the cowl ( ) on the transmission of the virus-laden aerosol particles in a drainage system of a typical 7-storeys residential building. The CFD models are first validated by the previous experiments in literature. Numerical results imply that the drainage system might play an essential role to the virus transmission. Then, results indicate that, the leakage risk of the aerosol particles via the floor drain with inefficient water-seal (UFD) mainly exists at the upper floors above the neutral pressure level (NPL). Besides, the negative and positive pressures at the bathroom can enhance and reduce the exposure risk of aerosol particles from the corresponding UFD, respectively. The increasing does not modify the location of the NPL. Moreover, the exposure risk of aerosol particles can be effectively avoided by the well water-sealed floor drains and/or the presence of a proper negative pressure at the cowl on the top floor. Finally, based on the CFD results, several protection suggestions on the drainage system and human activities are provided.
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http://dx.doi.org/10.1016/j.buildenv.2021.108009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161830PMC
August 2021

Tau-Targeted Multifunctional Nanoinhibitor for Alzheimer's Disease.

ACS Appl Mater Interfaces 2021 May 17;13(20):23328-23338. Epub 2021 May 17.

State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials of Ministry of Education, College of Chemistry, Nankai University, Tianjin 300071, P. R. China.

With the failure of various amyloid-β-targeted drugs for Alzheimer's disease (AD) in clinical trials, tau protein has gained growing attention as an alternative therapeutic target in recent years. The aggregation of tau exerts neurotoxicity, and its spreading in the brain is associated with increasing severity of clinical symptoms for AD patients; thus tau-targeting therapies hold great potential against AD. Here, a tau-targeted multifunctional nanoinhibitor based on self-assembled polymeric micelles decorated with tau-binding peptide is devised for AD treatment. Through the multivalent binding effect with the aggregating protein, this nanoinhibitor is capable of efficiently inhibiting tau protein aggregation, recognizing tau aggregates, and blocking their seeding in neural cells, thus remarkably mitigating tau-mediated cytotoxicity. Moreover, the formed nanoinhibitor-tau complex after binding is more easily degraded than mature tau aggregates, which will be conducive to enhance the therapeutic effect. We believe that this multifunctional nanoinhibitor will promote the development of new antitau strategies for AD treatment.
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http://dx.doi.org/10.1021/acsami.1c00257DOI Listing
May 2021

Codonopsis Radix modulates water and electrolytes homeostasis in mice.

Authors:
Shu Chen Xiaohui Wu

Heliyon 2021 Apr 22;7(4):e06735. Epub 2021 Apr 22.

State Key Laboratory of Genetic Engineering and National Center for International Research of Development and Disease, Institute of Developmental Biology and Molecular Medicine, Collaborative Innovation Center of Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China.

Codonopsis Radix is a traditional Chinese medicine best known for its effects in treating digestive, cardiovascular, immunological and hematopoitic diseases. It also appears in the traditional Chinese medical prescriptions against ascites. However, the physiological effect and molecular mechanism of Codonopsis Radix in water and electrolytes homeostasis have not been well studied. We found that Codonopsis Radix decoction increased water intake and the urine volume, but decreased food intake in mice. The treatment significantly reduced angiotensin II receptor (AT1R) transcription and serum aldosterone level in animals, suggested perturbed function of renin-angiotensin system. RNAseq analysis of Codonopsis Radix treated NCI-H295R cells detected suppressed AT1R, SP1, and TEF transcription as well. Thus, Codonopsis Radix may regulate water and electrolytes homeostasis by affecting AT1R expression and aldosterone biosynthesis, possibly through downregulating SP1 and TEF transcription.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093420PMC
April 2021

Hypothalamic-Pituitary-End-Organ Axes: Hormone Function in Female Patients with Major Depressive Disorder.

Neurosci Bull 2021 Aug 28;37(8):1176-1187. Epub 2021 Apr 28.

Clinical Research Center, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.

Classic hypothalamic-pituitary-end-organ feedback loops - the hypothalamic-pituitary-adrenal axis (HPAA), hypothalamic-pituitary-thyroidal axis (HPTA), and hypothalamic-pituitary-gonadal axis (HPGA) - are associated with the neuroendocrine and immune systems in major depressive disorder (MDD). Female patients with MDD present with evident neuroendocrine and immunological changes. Glucocorticoid, thyroid hormone, and reproductive steroid levels fluctuate with menstrual cycles, which might lead to glucocorticoid receptor resistance, impairment of triiodothyronine conversion, and sex hormone secretion disorders. In this review, we summarize the independent and interactive functions of these three axes in female MDD patients. The similar molecular structure of steroids implies an interrelationship between the hypothalamic-pituitary-end-organ axes and the competitive inhibitory effects at the receptor level, especially when considering the HPAA and HPGA.
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http://dx.doi.org/10.1007/s12264-021-00689-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353070PMC
August 2021

Comparing the Value of Cystatin C and Serum Creatinine for Evaluating the Renal Function and Predicting the Prognosis of COVID-19 Patients.

Front Pharmacol 2021 22;12:587816. Epub 2021 Mar 22.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Coronavirus disease- (COVID-19-) related renal function abnormality is associated with poor prognosis. However, the clinical significance of dynamic changes in renal function indicators has not been studied, and no studies have evaluated the renal function in COVID-19 patients by cystatin C. This study aimed to evaluate the effect of abnormal renal function on admission on prognosis of COVID-19 patients and the prognostic value of various renal function indicators. A total of 1,764 COVID-19 patients without a history of chronic kidney disease were categorized into two groups, an elevated cystatin C group and a normal cystatin C group, based on the results of renal function tests on admission. The clinical characteristics were compared between the two groups, and logistic or Cox regression analyses were performed to explore the associations between elevated cystatin C/serum creatinine levels and disease severity and survival. We also performed receiver operating characteristic (ROC) curve, Kaplan-Meier survival, and curve fitting analyses. When adjusted for several significant clinical variables, elevated cystatin C levels on admission were independent predictors of disease severity ( < 0.001), and elevated creatinine levels were independent predictors of death ( = 0.020). Additionally, the ROC curve analysis shows that elevated cystatin C levels [area under the curve (AUC): 0.656] have a better predictive value for disease severity than elevated creatinine levels (AUC: 0.540). The survival curves of patients with elevated cystatin C/creatinine levels show a sharper decline than those of patients with normal cystatin C/creatinine levels ( < 0.001). The curve fitting analysis revealed that, compared to the flat curves of cystatin C and creatinine levels for patients who survived, the curves for patients who died kept rising, and cystatin C levels rose above the normal range earlier than creatinine. Elevated cystatin C, which occurs earlier than serum creatinine, is useful for the early detection of renal function abnormality and might have better predictive value for disease severity in COVID-19 patients, while elevated serum creatinine may have a better predictive value for risks of death.
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http://dx.doi.org/10.3389/fphar.2021.587816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019901PMC
March 2021

Comparing the Value of Cystatin C and Serum Creatinine for Evaluating the Renal Function and Predicting the Prognosis of COVID-19 Patients.

Front Pharmacol 2021 22;12:587816. Epub 2021 Mar 22.

Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Coronavirus disease- (COVID-19-) related renal function abnormality is associated with poor prognosis. However, the clinical significance of dynamic changes in renal function indicators has not been studied, and no studies have evaluated the renal function in COVID-19 patients by cystatin C. This study aimed to evaluate the effect of abnormal renal function on admission on prognosis of COVID-19 patients and the prognostic value of various renal function indicators. A total of 1,764 COVID-19 patients without a history of chronic kidney disease were categorized into two groups, an elevated cystatin C group and a normal cystatin C group, based on the results of renal function tests on admission. The clinical characteristics were compared between the two groups, and logistic or Cox regression analyses were performed to explore the associations between elevated cystatin C/serum creatinine levels and disease severity and survival. We also performed receiver operating characteristic (ROC) curve, Kaplan-Meier survival, and curve fitting analyses. When adjusted for several significant clinical variables, elevated cystatin C levels on admission were independent predictors of disease severity ( < 0.001), and elevated creatinine levels were independent predictors of death ( = 0.020). Additionally, the ROC curve analysis shows that elevated cystatin C levels [area under the curve (AUC): 0.656] have a better predictive value for disease severity than elevated creatinine levels (AUC: 0.540). The survival curves of patients with elevated cystatin C/creatinine levels show a sharper decline than those of patients with normal cystatin C/creatinine levels ( < 0.001). The curve fitting analysis revealed that, compared to the flat curves of cystatin C and creatinine levels for patients who survived, the curves for patients who died kept rising, and cystatin C levels rose above the normal range earlier than creatinine. Elevated cystatin C, which occurs earlier than serum creatinine, is useful for the early detection of renal function abnormality and might have better predictive value for disease severity in COVID-19 patients, while elevated serum creatinine may have a better predictive value for risks of death.
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http://dx.doi.org/10.3389/fphar.2021.587816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8019901PMC
March 2021

Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress.

Free Radic Biol Med 2021 05 1;168:247-257. Epub 2021 Apr 1.

School of Medicine, Nanjing University of Chinese Medicine, 210046, Nanjing, China; Collaborative Innovation Center of Jiangsu Province of Cancer Prevention and Treatment of Chinese Medicine, 210023, Nanjing, China; International Medical College, Zhejiang Shuren University, 310022, Hangzhou, China. Electronic address:

Methicillin-resistant Staphylococcus aureus (MRSA) is the leading cause of bacterial pneumonia, featured with exuberant inflammatory cytokine production, extensive oxidative stress and tissue injury. The Keap1/Nrf2 system is the major apparatus essential for host defense against oxidative and electrophilic stresses of both exogenous and endogenous origins, representing a logical target for host-directed strategy to treat severe inflammatory diseases including MRSA-induced pneumonia. In an effort to search therapeutics for bacterial pneumonia, we identify rosmarinic acid (RA) as a covalent modifier of Keap1 and hence an activator of Nrf2. Specifically, RA forms a covalent bond with the cysteine 151 of Keap1 in BTB domain, and blocks its association with Nrf2 for proteasome-mediated degradation. Consequently, RA treatment caused the increased Nrf2 nuclear translocation to initiate antioxidant and mitochondrial biogenic programs, as well as macrophage bactericidal activity through inducing autophagic pathway, which eventually led to expedited bacterial eradication, inflammation resolution, and disease recovery. Collectively, our findings establish RA as a specific inducer of Nrf2 and show its potential to prevent MRSA pneumonia.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.03.038DOI Listing
May 2021

Consumption of chilies and sweet peppers is associated with lower risk of sarcopenia in older adults.

Aging (Albany NY) 2021 03 26;13(6):9135-9142. Epub 2021 Mar 26.

Nutritional Epidemiology Institute and School of Public Health, Tianjin Medical University, Tianjin, China.

Background: Sarcopenia is an aging-related loss of muscle mass and function, which induces numerous adverse outcomes. Capsaicin and capsiate, separately extracted from chilies and sweet peppers, have the potential to induce muscle hypertrophy via activation of transient receptor potential vanilloid 1. The present study aimed to investigate whether chili and sweet pepper consumption are related to sarcopenia in the elderly general population.

Methods: A cross-sectional study with 2,451 participants was performed. Dietary chili and sweet pepper consumption were assessed using a validated self-administered food frequency questionnaire. Sarcopenia was defined according to the consensus of the Asian Working Group for Sarcopenia. Logistic regressions were performed to measure the effect of chili and sweet pepper consumption on sarcopenia.

Results: The prevalence of sarcopenia was 16.1%. After adjustment for potential confounding variables, the odds ratios (95% confidence intervals) for sarcopenia across chili and sweet pepper consumption categories were 1.00 (reference) for almost never, 0.73 (0.55, 0.97) and 0.73 (0.56, 0.96) for ≤1 time/week, 0.60 (0.39, 0.90) and 0.66 (0.45, 0.95) for ≥2-3 times/week (both for trend <0.01), respectively.

Conclusion: The present study showed that higher consumption of chilies and sweet peppers was related to a lower risk of sarcopenia in older adults.
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http://dx.doi.org/10.18632/aging.104168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034967PMC
March 2021

Association of exposure to ambient air pollution with ovarian reserve among women in Shanxi province of north China.

Environ Pollut 2021 Jun 3;278:116868. Epub 2021 Mar 3.

College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi, 030006, China. Electronic address:

Air pollution has been an important risk factor for female reproductive health. However, epidemiological evidence of ambient air pollution on the predictor for ovarian reserve (antral follicle count, AFC) is deficient. We aim to comprehensively evaluate the association of long-term exposure to ambient air pollution with AFC among women of reproductive age in Shanxi of north China. 600 women with spontaneous menstrual cycle, not using controlled ovarian stimulation, were enrolled in the retrospective study. Two distinct periods of antral follicle development were designed as exposure windows. Generalized linear model was employed to estimate the change of AFC associated with exposure of atmospheric pollutants (SO, NO, PM, PM, CO and O). Stratification analysis based on age (<30, ≥30 years), university degree (yes, no), years of exposure (2013-2016, 2017-2019) and duration of infertility (<2, 2-5, >5 years) along with two pollutants model were employed to further illustrate the association. We found every 10 μg/m increase in SO concentration level during the entire development stage of antral follicle was associated with a -0.01 change in AFC (95% confidence interval: -0.016, -0.002) adjusting for the confounders including age, BMI, parity and infertility diagnosis factors. The significant association of increased SO level with decreased AFC was particularly observed during the early transition from primary follicle to preantral follicle stage by 10 μg/m increase in SO exposure level with a -0.01 change (95% CI: -0.015, -0.002) in AFC. The negative association was pronounced among women aged ≥30 years old, and also significant in two pollutants model after adjusting the confounders. No significant associations between other air pollutants and AFC were observed. Our finding suggests that long-term exposure to air pollutant SO is associated with lower AFC, raising our concern that atmospheric SO exposure may have potential adverse impact on women ovarian reserve.
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http://dx.doi.org/10.1016/j.envpol.2021.116868DOI Listing
June 2021
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