Publications by authors named "Xiaohui Wang"

858 Publications

Preparative separation and purification of loliolide and epiloliolide from Ascophyllum nodosum using amine-based microporous organic polymer for solid phase extraction coupled with macroporous resin and prep-HPLC.

Anal Methods 2021 04 8;13(16):1939-1944. Epub 2021 Apr 8.

State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong First Medical University, Shandong Academy of Medical Sciences, Qingdao 266071, China.

Herein, we reported a novel approach for the preparative separation and purification of loliolide and epiloliolide from Ascophyllum nodosum. An amine-based microporous organic polymer (MOP) was used for the pretreatment of the nodosum extract via solid-phase extraction (SPE). The obtained extract was further purified using macroporous resin chromatography and preparative high-performance liquid chromatography (prep-HPLC). The loading and elution parameters of the MOP were evaluated using standard loliolide, and the optimized conditions were used during the SPE of the nodosum extract (37.5 g). After the pretreatment with MOP, the extract (2.79 g) was obtained and further purified using a D101 resin column followed by prep-HPLC. A pair of epimers were isolated and identified as loliolide (5.83 mg) and epiloliolide (2.50 mg) using high-resolution electrospray ionization tandem mass spectrometry (HRESI-MS), 1D- and 2D-nuclear magnetic resonance (NMR) spectroscopy. This study demonstrates the potential of MOPs in the separation and purification of monoterpenoids from complex plant samples.
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http://dx.doi.org/10.1039/d1ay00186hDOI Listing
April 2021

Neurokinin-1-tachykinin receptor agonist promotes diabetic fracture healing in rats with type 1 diabetes via modulation of Wnt/β-catenin signalling axis.

Saudi J Biol Sci 2021 Apr 17;28(4):2139-2145. Epub 2021 Feb 17.

Department of Geriatrics, Hengshui People's Hospital, Hengshui, Hubei 053000, China.

Diabetes mellitus is an ill-famed metabolic disorder with varied repercussions including delayed fracture healing. Wnt/β-catenin axis is known to play a tight pivotal role in the bone healing process. Substance P (SubP) is a neuropeptide with established positive modulatory functions in fracture healing and associated neuronal milieu. In this study, we performed local delivery of recombinant adenovirus of Dickkopf-1 (DKK1) into the fracture site to understand the antagonizing the role of DKK1 against substance P. Rats were segregated into 4 groups: (i) Fractured non-diabetic rats; (ii) Fractured T1D rats; T1D was provoked by using STZ 50 mg/kg for 5 consecutive days; (iii) Fractured T1D + SubP (50 mg/ml/Kg; i.p.; 30 min prior to fracture procedure); (iv) Fractured T1D + SubP + Ad-DKK1. Bone radiographs were taken using a Faxitron X-ray machine and the residual gap size was measured using an electric caliper. Western blotting was also performed to determine the protein expression levels of osteogenic markers (RUNX2, OSTX and OSTC) bone resorption markers (OPG, RANKL and RANK) and also Wnt-signalling markers (β-catenin, LRP5 and GSK-3β). We observed that SubP promoted osteogenesis (as indicated by RUNX2, OSTX and OSTC upregulation) and mitigated the bone resorption (as indicated by optimized OPG/RANKL/RANK axis) via activated Wnt signalling (manifested by upmodulated β-catenin and LRP5, with downmodulated GSK-3β levels. Activation of endogenous SubP or administration of exogenous mimics might counter-protect the fractured bone against the deforming effects of T1D.
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http://dx.doi.org/10.1016/j.sjbs.2021.02.026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071892PMC
April 2021

High Structural Stability of Photonic Crystals on Textile Substrates, Prepared a Surface-Supported Curing Strategy.

ACS Appl Mater Interfaces 2021 Apr 19;13(16):19221-19229. Epub 2021 Apr 19.

Engineering Research Centre for Eco-Dyeing and Finishing of Textiles, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Over the past years, photonic crystals (PCs) with a periodically ordered nanostructure have attracted great attention due to their potential as advanced optical materials for structural coloration of textiles. However, the weak structural stability of PCs on flexible textile substrates makes them vulnerable to strong external forces, hampering their large-scale application. In this work, a waterborne polyurethane (wPU) is chosen for enhancing the structural stability of PCs. The composite PCs (PCs/wPU) show both brilliant structural colors and significantly improved structural stability. The structural color produced by the encapsulated PCs is found to depend on the properties of encapsulating agents. The wPU with high surface tension solidifies mainly on the PC surface in the form of a transparent film, protecting the overall structure of PCs. Meanwhile, a small amount of wPU, infiltrating into the interior of PCs, provides strong adhesion and ensures stability among nanospheres. In turn, polydimethylsiloxane (PDMS) with low surface tension is easy to infiltrate into the interior of PCs, forming fully encapsulated PCs. This reduces the brightness of structural color produced by the final PCs/PDMS composite over the original PCs, due to the replacement of air by PDMS, and thus the decrease in the refractive index contrast of PCs. The supported curing strategy using the encapsulating agent with high surface tension is shown to not only improve the structural stability of PCs but also exert almost no influence on the optical properties of PCs, facilitating the practice application of structural coloration in the textile industry.
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http://dx.doi.org/10.1021/acsami.1c00176DOI Listing
April 2021

Cannabidiol-dihydroartemisinin conjugates for ameliorating neuroinflammation with reduced cytotoxicity.

Bioorg Med Chem 2021 Apr 2;39:116131. Epub 2021 Apr 2.

Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Department of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China. Electronic address:

Cannabidiol (CBD) and dihydroartemisinin (DHA) can alleviate neuroinflammatory responses. However, they show cytotoxicity, which severely limits their therapeutic windows. Therefore, there is a great need to develop neuroprotective agents with improved safety. Drug-drug conjugate is an emerging approach for enhancing therapeutic index. Herein, the development, synthesis, and the pharmacological characterization of CBD-DHA conjugates were performed. Meanwhile, the combination of CBD and DHA as separate entities was also quantitatively analyzed for direct comparison with CBD-DHA conjugates. In this study, BV-2 microglial cell line was used to mimic primary microglia and the effects of CBD, DHA, the combination of CBD and DHA, as well as CBD-DHA conjugates on LPS-activated signaling molecules and pro-inflammatory factors were assessed. The interaction of CBD and DHA in inhibiting LPS-induced nitric oxide (NO) production was found to be additive. In contrast, DHA was found to synergize with CBD in inhibiting BV-2 cellular viability which implies that the combination of CBD and DHA amplifies their cytotoxicity. CBD-DHA conjugate C3D eliminated the cytotoxicity associated with single CBD/DHA use without significantly compromising the anti-neuroinflammation activity. C3D was more potent than C2D and C4D in inhibiting LPS-induced NO and mRNAs of iNOS and IL-1β, which implies that the linker length is critical for CBD-DHA conjugates' anti-inflammatory activities. Further signaling characterizations showed that C3D inhibited LPS-induced NF-κB but not MAPKs activation in BV-2 cells, therefore blocking LPS-induced neuroinflammation. This work provides a good example that conjugated drug-drug approach may improve the therapeutic index by increasing the maximum tolerated concentration/dose compared to traditional combination strategy.
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http://dx.doi.org/10.1016/j.bmc.2021.116131DOI Listing
April 2021

Clinical significance of serum transforming growth factor-β1 and procollagen type I N-propeptide in post-tuberculosis tracheobronchial stenosis.

Exp Ther Med 2021 Jun 29;21(6):570. Epub 2021 Mar 29.

Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.

Non-invasive strategies for monitoring post-tuberculosis (TB) tracheobronchial stenosis (PTTS) are clinically important but currently lacking. Transforming growth factor-β1 (TGF-β1) and procollagen type I N-propeptide (PINP) have been identified as markers of fibrosis. The present study aimed to investigate the clinical significance of serum TGF-β1 and PINP in PTTS. Serum samples were collected from 119 patients with tracheobronchial TB after the condition was treated for at least 6 months (59 patients with airway stenosis and 60 patients with no stenosis). Serum TGF-β1 and PINP levels were measured using ELISA and compared between the groups. Relationships between serum TGF-β1 and PINP levels and clinical characteristics, interventional bronchoscopy and outcomes of airway stenosis were analysed. The correlation between TGF-β1 and PINP, and their diagnostic efficacy for airway stenosis were also analysed. The TGF-β1 and PINP levels in the airway stenosis group were higher than those in the non-stenosis group. Furthermore, airway stenosis with atelectasis or mucus plugging was associated with higher TGF-β1 levels, and airway stenosis with atelectasis, mucus plugging, right main bronchus stenosis or severe airway tracheal stenosis was associated with higher PINP levels. In addition, TGF-β1 and PINP levels increased after interventional bronchoscopy therapy and airway stenosis with recurrent stenosis was associated with higher baseline levels of both markers. Finally, TGF-β1 levels were positively correlated with PINP levels in patients with airway stenosis. The area under the receiver operating characteristic curve of TGF-β1 and PINP for distinguishing airway stenosis from non-stenosis cases was 0.824 (95% CI: 0.748-0.900) and 0.863 (95% CI: 0.796-0.930), respectively. Therefore, TGF-β1 and PINP are potential biomarkers that may be useful for diagnosing and monitoring PTTS.
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http://dx.doi.org/10.3892/etm.2021.10002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027726PMC
June 2021

KIF4A promotes the development of bladder cancer by transcriptionally activating the expression of CDCA3.

Int J Mol Med 2021 Jun 13;47(6). Epub 2021 Apr 13.

Department of Urology, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China.

Bladder cancer (BC) is among the most common urinary system tumors with a high morbidity and mortality worldwide. Despite advancements being made in the diagnosis and treatment of bladder cancer, targeted therapy remains the most promising treatment, and novel therapeutic targets are urgently required in to improve the outcomes of patients with BC. Kinesin family member 4A (KIF4A) is a plus‑end directed motor protein involved in the regulation of multiple cellular processes, such as mitosis and axon growth. Notably, KIF4A plays important roles in tumor growth and progression, and its expression is associated with the prognosis of several types of cancer. However, the potential role and molecular mechanisms of KIF4A in bladder cancer development remain unclear. The present study demonstrated that KIF4A was highly expressed in human BC tissues, and its expression was associated with patient clinicopathological characteristics, such as tumor stage (P=0.012) and with the prognosis of patients with BC. It was further found that KIF4A promoted the cell proliferation of bladder cancer both and . On the whole, the data presented herein provide evidence that KIF4A promotes the development of BC through the transcriptional activation of the expression of CDCA3. The present study indicates the involvement of KIF4A in the progression of BC and suggests that KIF4A may be a promising therapeutic target for the treatment of BC.
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http://dx.doi.org/10.3892/ijmm.2021.4932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041479PMC
June 2021

Clinical Features and Outcomes of Anti-N-Methyl-d-Aspartate Receptor Encephalitis in Infants and Toddlers.

Pediatr Neurol 2021 Mar 12;119:27-33. Epub 2021 Mar 12.

Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Department of Neurology, Beijing, China. Electronic address:

Objective: We describe the clinical features and outcomes of anti-N-methyl-d-aspartate receptor (NMDAR) encephalitis in infants and toddlers.

Methods: This was a single-center retrospective study. Infants and toddlers who met the diagnostic criteria for anti-NMDAR encephalitis were recruited for the study. Data on clinical features, treatment, and long-term outcomes were collected retrospectively.

Results: A total of 41 patients (age range: six to 34 months; median age: 23 months; female: 19) were enrolled in this study. Nineteen (46%) patients exhibited classical anti-NMDAR encephalitis, whereas 22 (54%) patients exhibited anti-NMDAR encephalitis after viral encephalitis. There was a high presentation of movement disorders (100%), developmental regression (90%), abnormal behaviors (90%). All patients were administered first-line therapy, with only 17% of them being administered second-line immunotherapy. Two patients succumbed to the disease, whereas none of them relapsed. At the long-term follow-up (more than one year), 20 of 35 (57%) exhibited satisfactory outcomes (modified Rankin Scale ≤2). Compared with patients with classical anti-NMDAR encephalitis (n = 18), patients after viral encephalitis (n = 17) were more likely to have worse clinical outcomes. They exhibited a higher modified Rankin Scale/Pediatric Cerebral Performance Category score and more frequent seizures. A predictor of poor outcome was presentation after viral encephalitis (odds ratio 35.7, 95% confidence interval 4.64 to 275.03, P = 0.001).

Conclusion: Anti-NMDAR encephalitis in infants and toddlers clinically presents with movement disorders, developmental regression, and abnormal behaviors. Interestingly, this group had a higher proportion of patients after viral encephalitis, which is regarded as the only risk factor for poor outcomes.
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http://dx.doi.org/10.1016/j.pediatrneurol.2021.02.009DOI Listing
March 2021

Responses of microbial communities and their interactions to ibuprofen in a bio-electrochemical system.

J Environ Manage 2021 Jul 2;289:112473. Epub 2021 Apr 2.

Beijing Engineering Research Center of Environmental Material for Water Purification, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China. Electronic address:

Ibuprofen has caused great concerns due to their potential environmental risks. However, their removal efficiency and their effects on microbial interactions in bio-electrochemical system remain unclear. To address these issues, a lab-scale bio-electrochemical reactor integrated with sulfur/iron-mediated autotrophic denitrification (BER-S/IAD) system exposing to 1000 μg L ibuprofen was operated for about two months. Results revealed that the BER-S/IAD system obtained efficient simultaneous denitrification (98.93%) and phosphorus (82.67%) removal, as well as an excellent ibuprofen removal performance (96.98%). Ibuprofen had no significant impacts on the nitrate (NO-N) removal and the ammonia (NH-N) accumulation, but decreased the total nitrogen (TN) and total phosphorus (TP) removal efficiencies. MiSeq sequencing analysis revealed that ibuprofen significantly (P < 0.05) decreased the microbial community diversity and changed their overall structure. Some bacteria related to denitrification and phosphorus removal, such as Pseudomonas and Thiobacillus, decreased significantly (P < 0.05). Moreover, molecular ecological network (MEN) analysis revealed that ibuprofen decreased the network's size and complexity, and enhanced the negative correlations of Proteobacteria and Firmicutes. Besides, ibuprofen decreased the links of some keystone bacteria related to denitrification and phosphorus removal. This research could provide a new dimension for our comprehending of the responses of microbial communities and their interactions to ibuprofen in bio-electrochemical system.
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http://dx.doi.org/10.1016/j.jenvman.2021.112473DOI Listing
July 2021

Behavior of Nutritional Supplements Use in Association With Inflammatory Skin Diseases in Chinese College Students.

Front Nutr 2021 17;8:615462. Epub 2021 Mar 17.

Mobile Joint Laboratory, Xiangya Hospital, Central South University, Changsha, China.

It is understudied how frequently adolescents use nutritional supplements (NS) and whether the corresponding behavior is associated with skin diseases that may cause unpleasant symptoms and disfigurement. The current study aimed to investigate the prevalence of NS use in Chinese college students and its association with inflammatory skin diseases. This was a university-based epidemiologic investigation that included 20,138 students who underwent dermatological examinations. A questionnaire survey was conducted to inquire about the use of NS along with related information. Skin diseases were diagnosed by dermatologists during the health examination. Logistic regression models were used for analysis. Adjusted odds ratios (aORs) were presented as the effect size. Survey responses from a total of 20,138 participants were analyzed. Specifically, 18.3% of the participants reported the use of NS in the past year. The use of vitamin C was most frequently reported, accounting for a proportion of 12.9%, followed by vitamin B and mineral supplements. The use of NS was found to be associated with female sex, Han ethnicity, higher annual household income, and a series of healthy lifestyles such as more physical activity, less second-hand smoke exposure, less alcohol consumption, and higher intake of milk and yogurt ( < 0.001). Participants with chronic urticaria (aOR = 1.3; 95% CI, 1.0-1.7), atopic dermatitis (aOR = 1.4; 95% CI, 1.2-1.6), or acne (aOR = 1.17; 95% CI, 1.04-1.31) were more likely to use NS, especially herbs (aOR = 2.7; 95% CI, 1.2-3.7), followed by vitamin B (aOR = 1.6; 95% CI, 1.2-2.0) and mineral supplements (aOR = 1.4; 95% CI, 1.0-2.0). College students with inflammatory skin diseases are more likely to use NS.
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http://dx.doi.org/10.3389/fnut.2021.615462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8010123PMC
March 2021

Adiponectin Enhances B-Cell Proliferation and Differentiation Activation of Akt1/STAT3 and Exacerbates Collagen-Induced Arthritis.

Front Immunol 2021 18;12:626310. Epub 2021 Mar 18.

Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Although B cells have been shown to contribute to the pathogenesis of rheumatoid arthritis (RA), the precise role of B cells in RA needs to be explored further. Our previous studies have revealed that adiponectin (AD) is expressed at high levels in inflamed synovial joint tissues, and its expression is closely correlated with progressive bone erosion in patients with RA. In this study, we investigated the possible role of AD in B cell proliferation and differentiation. We found that AD stimulation could induce B cell proliferation and differentiation in cell culture. Notably, local intraarticular injection of AD promoted B cell expansion in joint tissues and exacerbated arthritis in mice with collagen-induced arthritis (CIA). Mechanistically, AD induced the activation of PI3K/Akt1 and STAT3 and promoted the proliferation and differentiation of B cells. Moreover, STAT3 bound to the promoter of the Blimp-1 gene, upregulated Blimp-1 expression at the transcriptional level, and promoted B cell differentiation. Collectively, we observed that AD exacerbated CIA by enhancing B cell proliferation and differentiation mediated by the PI3K/Akt1/STAT3 axis.
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http://dx.doi.org/10.3389/fimmu.2021.626310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012765PMC
March 2021

Chronic lung inflammation and pulmonary fibrosis after multiple intranasal instillation of PM in mice.

Environ Toxicol 2021 Mar 29. Epub 2021 Mar 29.

Department of Pulmonary and Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Fine particulate matter (PM ) is an important component of air pollution and can induce lung inflammation and oxidative stress. We hypothesized that PM could play a role in the induction of pulmonary fibrosis. We examined whether multiple intranasal instillation of PM can induce pulmonary fibrosis in the mouse, and also investigated the underlying pro-fibrotic signaling pathways. C57/BL6 mice were intranasally instilled with 50 μl of PM suspension (7.8 μg/g body weight) or PBS three times a week over 3 weeks, 6 weeks or 9 weeks. To observe the recovery of pulmonary fibrosis after the termination of PM exposure, 9 week-PM instilled mice were also studied at 3 weeks after termination of instillation. There were significant decreases in total lung capacity (TLC) and compliance (Cchord) in the 9-week PM -instilled mice, while there were increased histological fibrosis scores with enhanced type I collagen and hydroxyproline deposition, increased mitochondrial ROS levels and NOX activity, decreased total SOD and GSH levels, accompanied by decreased mitochondrial number and aberrant mitochondrial morphology (swelling, vacuolization, cristal disruption, reduced matrix density) in PM -instilled mice. Multiple PM instillation resulted in increased expression of TGFβ1, increases of N-Cadherin and Vimentin and a decrease of E-Cadherin. It also led to decreases in OPA1 and MFN2, and increases in Parkin, SQSTM1/p62, the ratio of light china (LC) 3B II to LC3B I, PI3k/Akt phosphorylation, and NLRP3 expression. Intranasal instillation of PM for 9 weeks induced lung inflammation and pulmonary fibrosis, which was linked with aberrant epithelial-mesenchymal transition, oxidative stress, mitochondrial damage and mitophagy, as well as activation of TGFβ1-PI3K/Akt, TGFβ1- NOX and TGFβ1-NLRP3 pathways.
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http://dx.doi.org/10.1002/tox.23140DOI Listing
March 2021

Quantitative H NMR for the direct quantification of saikosaponins in Bupleurum chinense DC.

Anal Sci 2021 Mar 26. Epub 2021 Mar 26.

School of Pharmacy and Chemical Engineering, Zhengzhou University of Industrial Technology.

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http://dx.doi.org/10.2116/analsci.20P462DOI Listing
March 2021

Host-derived lipids orchestrate pulmonary γδ T cell response to provide early protection against influenza virus infection.

Nat Commun 2021 03 26;12(1):1914. Epub 2021 Mar 26.

Department of Pathology and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Hong Kong, China.

Innate immunity is important for host defense by eliciting rapid anti-viral responses and bridging adaptive immunity. Here, we show that endogenous lipids released from virus-infected host cells activate lung γδ T cells to produce interleukin 17 A (IL-17A) for early protection against H1N1 influenza infection. During infection, the lung γδ T cell pool is constantly supplemented by thymic output, with recent emigrants infiltrating into the lung parenchyma and airway to acquire tissue-resident feature. Single-cell studies identify IL-17A-producing γδ T (Tγδ17) cells with a phenotype of TCRγδCD3AQP3CXCR6 in both infected mice and patients with pneumonia. Mechanistically, host cell-released lipids during viral infection are presented by lung infiltrating CD1d B-1a cells to activate IL-17A production in γδ T cells via γδTCR-mediated IRF4-dependent transcription. Reduced IL-17A production in γδ T cells is detected in mice either lacking B-1a cells or with ablated CD1d in B cells. Our findings identify a local host-immune crosstalk and define important cellular and molecular mediators for early innate defense against lung viral infection.
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http://dx.doi.org/10.1038/s41467-021-22242-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7997921PMC
March 2021

Three-Dimensional Printing-Assisted Masquelet Technique in the Treatment of Calcaneal Defects.

Orthop Surg 2021 Mar 25. Epub 2021 Mar 25.

Luoyang Orthopedic-Traumatological Hospital of Henan Province (Henan Provincial Orthopedic Hospital), Luoyang Henan, P.R. China.

Objective: The aim of the present study was to summarize the clinical efficacy of three-dimensional (3D) printing technology combined with the Masquelet technique in the treatment of calcaneal defects.

Methods: From January 2018 to April 2019, 3D printing combined with induced masquelet technology was used to treat four patients with calcaneal defects, including two men and two women. The patients were aged 22-52 years old, with an average age of 36 years. There were two cases of traffic accident injuries, there was one case of a fall from height, and there was one case of crush injury. CT scans were used to reconstruct the bilateral calcaneus, mirror technology was used to construct the bone defect area, and Materialise 3-matic software was used to design the calcaneus shaper mold and 3D print the mold. During the operation, the mold was used to shape the bone cement and fill the bone defect. In the second stage, the bone cement was removed and autologous bone was implanted to repair the bone defect. All patients were followed up to observe the effect.

Results: All four patients were followed up for 14 months (range, 10-18 months). There were three cases of infectious bone defects: two cases of Escherichia coli and one case of Pseudomonas aeruginosa. The 3D printed mold was used to shape the bone cement. During the operation, it was found to have a high degree of matching with the defect area of calcaneus. There is no need to adjust it again, and the wound healed well after the first stage. In the second stage of surgery, it was found that the induced membrane formed was complete and of appropriate size; the bone cement was easily removed during the operation. The fracture healing time was 3-6 months, with an average of 4 months. At the last follow up, there was no pain and the patients walked with full weight bearing. The Maryland score was 94 points (range, 88-98 points); three cases were excellent and one case was good. The AOFAS score ranged from 86 to 98, with an average of 92.8 points; three cases were excellent and one case was good.

Conclusion: Three-dimensional printing technology combined with induced membrane technology is an effective approach for treating calcaneal bone defects.
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http://dx.doi.org/10.1111/os.12873DOI Listing
March 2021

Self-assembly behavior and conformation of amphiphilic hemicellulose-graft-fatty acid micelles.

Carbohydr Polym 2021 Jun 3;261:117886. Epub 2021 Mar 3.

State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, PR China. Electronic address:

In this work, a series of amphiphilic hemicellulose-based grafting polymers are synthesized by homogeneous esterification with various hydrophobic chain fatty acids. With the flexible chain conformation of hemicelluloses, the hemicellulose-graft-fatty acid is self-assembled into hard sphere micelles in aqueous solution through hydrophobic interactions. The resultant micelles show apparent hydrodynamic radius (R) varying in the range of 34-57 nm and radius of gyration (R varying from 30 to 44 nm. Moreover, the R, R, zeta potential and critical micelle concentration (CMC) of the micelles gradually decreases with the increasing hydrophobic chain length. The aggregation number of micelles can be tuned by varying alkyl chain lengths. Taking curcumin (Cur) as a model drug, the hemicellulose micelles were investigated as drug carriers, which exhibit a chain length-controlled drug release behavior. Taken together, structure and property tunable hemicellulose-based micelles were obtained, and showed potential of application in pharmaceutical, food, and cosmetics fields.
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http://dx.doi.org/10.1016/j.carbpol.2021.117886DOI Listing
June 2021

A Bimetallic Metal-Organic Framework Encapsulated with DNAzyme for Intracellular Drug Synthesis and Self-Sufficient Gene Therapy.

Angew Chem Int Ed Engl 2021 Mar 19. Epub 2021 Mar 19.

Laboratory of Chemical Biology and State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P. R. China.

Although chemotherapy is one of the most widely used cancer treatments, there are serious side effects, drug resistance, and secondary metastasis. To address these problems, herein we designed a bimetallic metal-organic framework (MOF) encapsulated with DNAzyme for co-triggered in situ cancer drug synthesis and DNAzyme-based gene therapy. Once in cancer cells, MOFs would disassemble and liberate copper ions, zinc ions, and DNAzyme under the acidic environment of lysosomes. Copper ions can catalyze the synthesis of the chemotherapeutic drug through copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction after being reduced to Cu ; zinc ions act as the cofactor to activate the cleavage activity of DNAzyme. The anticancer drug is synthesized intracellularly and can kill cancer cells on site to minimize side effects to normal organisms. The activated DNAzyme starts gene therapy to inhibit tumor proliferation and metastasis by targeting and cleaving oncogene substrates.
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http://dx.doi.org/10.1002/anie.202016442DOI Listing
March 2021

Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis.

J Agric Food Chem 2021 Mar 15;69(11):3401-3414. Epub 2021 Mar 15.

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, Anhui 230036, People's Republic of China.

Tea leaves contain an extraordinarily high level of flavonoids that contribute to tea health benefits and flavor characteristics, but the regulatory mechanism of ambient ultraviolet B (UV-B) on tea flavonoid enrichment remains unclear. Here, we report that ambient UV-B modulates tea quality by inducing a metabolic flux in flavonoid biosynthesis. UV-B absence decreased bitter- and astringent-tasting flavonol glycosides (kaempferol-7--glucoside, myricetin-3--glucoside, and quercetin-7--glucoside) but increased non-galloylated catechins. Conversely, supplementary UV-B increased flavonols and decreased catechins in tea leaves. These responses were achieved via CsHY5, which mediates the UV-B-induced MYB12 activation and binds to the promoters of flavonoid biosynthetic genes (, , and ), leading to flavonoid changes. Transcriptomic data indicated that UV-B-induced tea flavonoid regulation is responsive to multiple biotic and abiotic environmental stresses. These findings improve our understanding of light-regulated tea astringency and bitterness underlying shading effects and seasonal light changes and provide novel insights into tea cultivation management and processing.
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http://dx.doi.org/10.1021/acs.jafc.0c07009DOI Listing
March 2021

Contralateral Lower Neck Sparing Radiotherapy in Stage N1 Nasopharyngeal Carcinoma: Long-Term Survival Outcomes and Late Toxicities.

Front Oncol 2021 25;11:628919. Epub 2021 Feb 25.

Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: To explore the feasibility of contralateral lower neck sparing radiotherapy for patients with stage N1 nasopharyngeal carcinoma (NPC) by analyzing long-term survival outcomes and late toxicities.

Methods: Data of patients with stage N1 NPC who were treated with contralateral lower neck sparing radiotherapy between January 2013 and December 2015 were analyzed. These patients were all staged by magnetic resonance imaging (MRI), and all received irradiation to the upper neck (levels II, III, and Va) bilaterally along with ipsilateral levels IV and Vb, without irradiation of the contralateral lower neck. Treatment outcomes, regional failure patterns, and late toxicities were examined.

Results: A total of 275 eligible patients with stage N1 NPC were included in the present study. The median follow-up period was 62 months (range, 3-93 months). The 5-year overall survival (OS), distant metastasis-free survival (DMFS), local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS), locoregional recurrence-free survival (LRRFS), and progression-free survival (PFS) rates were 90.5, 91.3, 94.7, 95.3, 91.2, and 81.7%, respectively. A total of 13 patients (4.7%) developed regional recurrence, all of which occurred in the field and not out of the field. Among 254 patients with available data on late toxicities, the most common late toxicity was xerostomia. No late injuries occurred in the carotid arteries, brachial plexus, or spinal cord. In addition to one case (0.4%) of neck fibrosis and three cases (1.2%) of hearing loss, there were no other grade 3-4 late toxicities observed.

Conclusions: Contralateral lower neck sparing radiotherapy would be safe and feasible for patients with stage N1 NPC, with the potential to improve the long-term quality of life of patients.
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http://dx.doi.org/10.3389/fonc.2021.628919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947858PMC
February 2021

A sandwich-like chitosan-based antibacterial nanocomposite film with reduced graphene oxide immobilized silver nanoparticles.

Carbohydr Polym 2021 May 18;260:117835. Epub 2021 Feb 18.

State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, 381 Wushan Road, Tianhe District, Guangzhou, 510640, China. Electronic address:

Bacterial breeding is the main cause of food perishability, which is harmful to human health. Silver nanoparticles (AgNPs) are one of the most widely used antimicrobial agents, but they are easy to release and cause cumulative toxicity. In this work, with corn stalk as green reductant and GO as template, a simple electrostatic self-assembled sandwich-like chitosan (CS) wrapped rGO@AgNPs nanocomposite film (CS/rGO@AgNPs) was synthesized to achieve stabilizing and controlled-release of AgNPs. The results showed that the the CS/rGO@AgNPs film continued releasing AgNPs for up to 14 days, and the final release amount of silver nanoparticles was only about 1.9 %. More importantly, the nanocomposite film showed durable antibacterial effect and good antibacterial activity against E. coli and S. aureus, and they showed no toxicity to cells. Hence, the nanocomposite film has potential application as an effective and safe packaging material to prolong the shelf life of food products.
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http://dx.doi.org/10.1016/j.carbpol.2021.117835DOI Listing
May 2021

Corrigendum to 'Analysis of suspended microplastics in the Changjiang Estuary: Implications for riverine plastic load to the ocean' [Water Res. 161 (2019) 560-569/48672].

Water Res 2021 May 9;195:116987. Epub 2021 Mar 9.

State Key Laboratory of Estuarine and Costal Research, East China Normal University, Shanghai 200062, China. Electronic address:

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http://dx.doi.org/10.1016/j.watres.2021.116987DOI Listing
May 2021

A thick semi-monolithic scintillator detector for clinical PET scanners.

Phys Med Biol 2021 Mar 12;66(6):065023. Epub 2021 Mar 12.

Center for Advanced Material Diagnostic Technology, Shenzhen Technology University, Shenzhen 518118, People's Republic of China. Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.

Both monolithic and semi-monolithic scintillator positron emission tomography (PET) detectors can measure the depth of interaction with single-ended readout. Usually scintillators with a thickness of 10 mm or less are used since the position resolutions of the detectors degrade as the scintillator thickness increases. In this work, the performance of a 20 mm thick long rectangular semi-monolithic scintillator PET detector was measured by using both single-ended and dual-ended readouts with silicon photomultiplier (SiPM) arrays to provide a high detection efficiency. The semi-monolithic scintillator detector consists of nine lutetium-yttrium oxyorthosilicate slices measuring 1.37 × 51.2 × 20 mm with erythrocyte sedimentation rate foils of 0.065 mm thickness in between the slices. The SiPM array at each end of the scintillator detector consists of 16 × 4 SiPMs with a pixel size of 3.0 × 3.0 mm and a pitch of 3.2 mm. The 64 signals of each SiPM array are processed by using the TOFPET2 application-specific integrated circuit individually. All but the edge slices can be clearly resolved for the detectors with both single-ended and dual-ended readouts. The single-ended readout detector provides an average full width at half maximum (FWHM) Y (continuous direction) position resolution of 2.43 mm, Z (depth direction) position resolution of 4.77 mm, energy resolution of 25.7% and timing resolution of 779 ps. The dual-ended readout detector significantly improves the Y and Z position resolutions, slightly improves the energy and timing resolution at the cost of two photodetectors required for one detector module and provides an average FWHM Y position resolution of 1.97 mm, Z position resolution of 2.60 mm, energy resolution of 21.7% and timing resolution of 718 ps. The energy and timing resolution of the semi-monolithic scintillator detector in this work are worse than those of the segmented scintillator array detector and need to be further improved. The semi-monolithic scintillator detector described in this work reduces costs as compared to the traditional segmented scintillator array detector and reduces the edge effect as compared to the monolithic scintillator detector.
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http://dx.doi.org/10.1088/1361-6560/abe761DOI Listing
March 2021

Preparation and Characterization of TiO₂ Nanowires Modified Organically with Coupling Agents.

J Nanosci Nanotechnol 2021 Sep;21(9):4870-4876

School of Chemical Engineering, Xuzhou Institute of Technology, Xuzhou 221111, China.

Anatase-type one-dimensional TiO₂ nanowire was prepared by hydrothermal method. The nanowires were modified by three kinds of silane coupling agents, such as KH550, KH560 and KH570. Flocculation was caused when the amount of modifier reached a certain level. When KH570 was used at 3.0 percent, at 80 °C, 4 h, and pH value between 9 and 10, modified nanowires had the highest 56.5 percent lipophilization degree, the lowest 0.562 per/nm² surface hydroxyl number, and the maximal 121.2° static contact angle.
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http://dx.doi.org/10.1166/jnn.2021.19152DOI Listing
September 2021

Current use of rivaroxaban in elderly patients with venous thromboembolism (VTE).

J Thromb Thrombolysis 2021 Mar 5. Epub 2021 Mar 5.

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Venous thromboembolism (VTE), which is characterized by pulmonary embolism and deep vein thrombosis, has become a serious public concern. Notably, over half of the patients with VTE are over 70 years of age, but elderly patients are at high risk of anti-coagulation and bleeding, which increase with age. Moreover, risk factors and frailty also show a difference between elderly patients and ordinary patients diagnosed with VTE. Rivaroxaban is a direct inhibitor of activated factor Xa and has the advantage of predictable pharmacodynamics and pharmacokinetics, no coagulation monitoring, and few drug interactions. As a first-line therapy for VTE, this drug is more advantageous than traditional therapy and exhibits good efficacy and safety for ordinary patients. However, the effectiveness and safety of rivaroxaban in elderly patients have not been fully elucidated. This article reviewed the use of rivaroxaban in elderly patients, including drug interactions, monitoring, reversal agents of rivaroxaban, and the use of small dosages of rivaroxaban in elderly patients.
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http://dx.doi.org/10.1007/s11239-021-02415-5DOI Listing
March 2021

The association of metabolic syndrome and cognitive impairment in Jidong of China: a cross-sectional study.

BMC Endocr Disord 2021 Mar 4;21(1):40. Epub 2021 Mar 4.

Department of Epidemiology, School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, 619 Changcheng Road, Taian, 271016, P.R. China.

Background: Metabolic syndrome (Mets) is prevalent in the general population and has been reported to be an independent risk factor for cognitive impairment. This study aimed to investigate the association of Mets with the risk of cognitive impairment.

Methods: We studied 5854 participants from the Jidong community. Cognitive function was assessed by the Mini-Mental State of Examination (MMSE) scale. Mets was diagnosed according to the International Diabetes Federation criteria. We used logistic regression analysis to investigate the association of metabolic syndrome with the risk of cognitive impairment.

Result: Among the 5854 adults included in the study, the age mean (SD) of age was 44 (13.57) years, and 2916 (50.34%) were male. There was a higher (56.03%) cognitive impairment incidence rate among participants with Mets than among those without Mets. In addition, there was a significant association between Mets and cognitive impairment (OR: 2.39, 95% CI: 2.00-2.86, P < 0.05) after adjusting for potential confounders, including age, gender, education level, marital status, smoking and alcohol consumption status. Regarding the 5 Mets components, abdominal obesity and elevated blood pressure were associated with the risk of Mets (OR: 1.36, 95% CI: 1.09-1.70, P < 0.001; OR: 1.32, 95% CI: 1.07-1.63, P < 0.05). Moreover, the strongest statistical correlation (adjusted OR: 1.86, 95% CI: 1.22-2.83, P < 0.05) was found when the number of Mets components was three.

Conclusion: Our study suggested that Mets was associated with cognitive impairment and that abdominal obesity and hypertension were associated with an increased risk of cognitive impairment.
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http://dx.doi.org/10.1186/s12902-021-00705-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934472PMC
March 2021

Efficient transport of atmospheric microplastics onto the continent via the East Asian summer monsoon.

J Hazard Mater 2021 Feb 22;414:125477. Epub 2021 Feb 22.

State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China. Electronic address:

The presence of microplastics (MPs) in the atmosphere is a global concern because of its environmental and health impacts; however, the monsoonal transport of atmospheric MPs has not yet been investigated. To fully understand the effect of the monsoon on atmospheric MP transport, we conducted a study along the southeast coast of China during the East Asian summer monsoon (EASM). We found that the EASM transports atmospheric MPs back onto the continent at a flux of up to 212.977-213.433 kg/EASM/year. The backward trajectory and wind field results indicate that the EASM provides an effective MP transport pathway from Vietnam, the Philippines, and Malaysia to southeastern China. This suggests that only some of the airborne MPs over the ocean enter the marine ecosystem. The average abundance of atmospheric MPs over the sampling area was 0.39 items/100 m (0.39 ± 0.43 items/100 m) during the EASM season, with high variability among the sampling sites. This study improves our understanding of the impact of the EASM on atmospheric MP transport, which can help quantify the contributions of atmospheric MPs to marine or terrestrial ecosystems.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125477DOI Listing
February 2021

Microbial composition and diversity of drinking water: A full scale spatial-temporal investigation of a city in northern China.

Sci Total Environ 2021 Jul 20;776:145986. Epub 2021 Feb 20.

School of Environment, Tsinghua University, Beijing 100084, China; Research Institute for Environmental Innovation (Suzhou) Tsinghua, Suzhou 215163, China. Electronic address:

The microbiological water quality of drinking water distribution systems (DWDSs) is of primary importance for public health. The detachment of biofilm attached on the pipe wall attribution to water source switch and the occurrence of potentially pathogenic chlorine-resistant bacteria (CRB) under chlorine disinfection get lots of attention. Studies examining microbial communities after the water source switch, particularly in low-salinity water, have been scant. The UV‑chlorine combined disinfection applied in one of the investigated drinking water plants provided insight into the control of CRBs. We applied high-throughput sequencing of the 16S rRNA gene to characterize the bacterial communities of the DWDS in northern China over 1 year. A network comprising four different DWDSs was sampled at 48 sites every season (temperate continental monsoon climate), and the impact of key spatial-temporal and physicochemical parameters was investigated. Overall, the entire bacterial community was not significantly different among the four DWDSs (spatial parameter) but varied with seasons (temporal parameter). The switch in water sources might increase the relative abundance of potentially opportunistic pathogens in DWDSs. UV‑chlorine combined disinfection can decrease community diversity and is likely to control the growth of potential opportunistic pathogens in DWDSs.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145986DOI Listing
July 2021

Family exposure and the impact of containment measures to children with coronavirus disease 2019 outside Hubei, China: a cross-sectional study.

Transl Pediatr 2021 Jan;10(1):92-102

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.

Background: In response to the ongoing epidemic of coronavirus disease 2019 (COVID-19), China has carried out restrictive disease containment measures across the country.

Methods: In this cross-sectional study, we collected demographic and epidemiological data of 376 laboratory-confirmed cases of COVID-19 among children younger than 18 years of age. Using descriptive statistics and odds ratios, we described the odds of exposure outside the family after the implementation of control measures compared to before.

Results: Children diagnosed on or after February 4, 2020, had a lower odds of exposure to COVID-19 outside of the family compared to those diagnosed before February 3, 2020 (OR =0.594, 95% CI: 0.391 to 0.904). In the stratified analysis, children aged 0 to 5 years had the lowest odds of exposure outside of the family (OR =0.420, 95% CI: 0.196 to 0.904) compared to the other age groups assessed.

Conclusions: Our study on the children infected with COVID-19 as well as their exposure within family provided evidence that the implementation of containment measures was effective in reducing the odds of exposure outside of the family, especially for preschool children. Continuation of these efforts, coupled with tailored prevention and health education messaging for younger aged children, may help to reduce the transmission of COVID-19 among children until other therapeutic interventions or vaccines are available.
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http://dx.doi.org/10.21037/tp-20-214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882296PMC
January 2021