Publications by authors named "Xiaohui Li"

827 Publications

Fano Resonance in Single-molecule Junctions.

Angew Chem Int Ed Engl 2022 Aug 18. Epub 2022 Aug 18.

Xiamen University, College of Chemistry and Chemical Engineering, Siming south road 422, 3012, Xiamen, CHINA.

The Fano resonance in single-molecule junctions could be created by interaction with discrete and continuous molecular orbitals and enables effective electron transport modulation between constructive and destructive interference within a small energy range. However, direct observation of Fano resonance remains unexplored because of the disappearance of discrete orbitals by molecule-electrode coupling. We demonstrated the room-temperature observation of Fano resonance from electrochemical gated single-molecule conductance and current-voltage measurements of a para -carbazole anion junction. Theoretical calculations reveal that the negative charge on the nitrogen atom induces a localized HOMO on the molecular center, creating Fano resonance by interfering with the delocalized LUMO on the molecular backbone. Our findings demonstrate that the Fano resonance in electron transport through single-molecule junctions opens pathways for designs of interference-based electronic devices.
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http://dx.doi.org/10.1002/anie.202210097DOI Listing
August 2022

LBS user location privacy protection scheme based on trajectory similarity.

Authors:
Kun Qian Xiaohui Li

Sci Rep 2022 Aug 17;12(1):13982. Epub 2022 Aug 17.

School of Electronics and Information Engineering, Liaoning University of Technology, Jinzhou, 121001, China.

During the data set input or output, or the data set itself adds noise to enable data distortion to effectively reduce the risk of user privacy leakage. However, in the conventional method, the added noise may cause data distortion, thereby appealed against it. However, the amount of noise is too small and cannot meet the effect of privacy protection. Therefore, we propose a LBS user location privacy protection scheme based on trajectory similarity (DPTS). With double privacy protection without reducing the efficiency of algorithms, it does not cause data distortion to provide more reliable privacy protection. The main contributions of this article include: (1) In the process of collecting and publishing the location data, introduce into the privacy protection method, (2) The differential privacy algorithm based on the trajectory prefix tree is superimposed on the basis of the false position replacement algorithm based on the trajectory similarity, (3) Propose LBS-based Difference Privacy Protection Algorithm. In the algorithm, We reach the purpose of protecting user personal privacy by replace the original trajectory into a fake track trace that is the lowest degree of similarity in the interval. Then establish a prefix tree and add noise to the positional frequency. It is in order to further protect the sensitive location information, double protection in the trajectory data set, and the degree of privacy protection is improved. Simulation experiment results show that the proposed algorithm is effective. The algorithm can suppress the distortion rate of data while improving the amount of noise, and in improving the algorithm operation efficiency, it reduces the risk of leakage of sensitive position information.
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http://dx.doi.org/10.1038/s41598-022-18268-8DOI Listing
August 2022

Protein Panel of Serum-Derived Small Extracellular Vesicles for the Screening and Diagnosis of Epithelial Ovarian Cancer.

Cancers (Basel) 2022 Jul 30;14(15). Epub 2022 Jul 30.

Department of Gynecology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.

Although ovarian cancer, a gynecological malignancy, has the highest fatality rate, it still lacks highly specific biomarkers, and the differential diagnosis of ovarian masses remains difficult to determine for gynecologists. Our study aimed to obtain ovarian cancer-specific protein candidates from the circulating small extracellular vesicles (sEVs) and develop a protein panel for ovarian cancer screening and differential diagnosis of ovarian masses. In our study, sEVs derived from the serum of healthy controls and patients with cystadenoma and ovarian cancer were investigated to obtain a cancer-specific proteomic profile. In a discovery cohort, 1119 proteins were identified, and significant differences in the protein profiles of EVs were observed among groups. Then, 23 differentially expressed proteins were assessed using the parallel reaction monitoring in a validation cohort. Through univariate and multivariate logistic regression analyses, a novel model comprising three proteins (fibrinogen gamma gene (FGG), mucin 16 (MUC16), and apolipoprotein (APOA4)) was established to screen patients with ovarian cancer. This model exhibited an area under the receiver operating characteristic curve (AUC) of 0.936 (95% CI, 0.888-0.984) with 92.0% sensitivity and 82.9% specificity. Another panel comprising serum CA125, sEV-APOA4, and sEV-CD5L showed excellent performance (AUC 0.945 (95% CI, 0.890-1.000), sensitivity of 88.0%, specificity of 93.3%, and accuracy of 89.2%) to distinguish malignancy from benign ovarian masses. Altogether, our study provided a proteomic signature of circulating sEVs in ovarian cancer. The diagnostic proteomic panel may complement current clinical diagnostic measures for screening ovarian cancer in the general population and the differential diagnosis of ovarian masses.
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http://dx.doi.org/10.3390/cancers14153719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367436PMC
July 2022

Polymer-complexed SnO electron transport layer for high-efficiency n-i-p perovskite solar cells.

Nanoscale 2022 Aug 11. Epub 2022 Aug 11.

Industrial Chemistry Program, Faculty of Science and Natural Resources, University Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia.

An effective electron transport layer (ETL) plays a pivotal role in suppressing nonradiative recombination at the interface as well as promoting perovskite crystallization to facilitate electron extraction in perovskite solar cells (PSCs). Herein, a functional polymer, poly(amidoamine) (PM) dendrimer, is introduced to rationalize the morphology and electrical performance of SnO nanocrystals to construct an SnO charge transfer layer. PM offers an even SnO colloidal dispersion with a particle-size distribution of ∼10 nm, which prevents the agglomeration of nanocrystals significantly. The polymer-complexed SnO provides a uniform and dense ETL film without island-like agglomeration, yielding a large conductive layer superior to that of the control. Equally important, the wettability-improved SnO ETL with PM modification produces a high-quality perovskite film with larger grain size, resulting in a power conversion efficiency (PCE) of the champion n-i-p PSC of up to 22.93% with negligible hysteresis. Noticeably, the device based on SnO-PM maintained 71% of its initial PCE (only 49% for the control device) after storing in the ambient environment for 45 days (relative humidity of 30%-80%) without packaging.
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http://dx.doi.org/10.1039/d2nr03754hDOI Listing
August 2022

A novel quantitative prognostic model for initially diagnosed non-small cell lung cancer with brain metastases.

Cancer Cell Int 2022 Aug 11;22(1):251. Epub 2022 Aug 11.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, 510060, People's Republic of China.

Background: The prognosis of non-small cell lung cancer (NSCLC) with brain metastases (BMs) had been researched in some researches, but the combination of clinical characteristics and serum inflammatory indexes as a noninvasive and more accurate model has not been described.

Methods: We retrospectively screened patients with BMs at the initial diagnosis of NSCLC at Sun Yat-Sen University Cancer Center. LASSO-Cox regression analysis was used to establish a novel prognostic model for predicting OS based on blood biomarkers. The predictive accuracy and discriminative ability of the prognostic model was compared to Adjusted prognostic Analysis (APA), Recursive Partition Analysis (RPA), and Graded Prognostic Assessment (GPA) using concordance index (C-index), time-dependent receiver operating characteristic (td-ROC) curve, Decision Curve Analysis(DCA), net reclassification improvement index (NRI), and integrated discrimination improvement index (IDI).

Results: 10-parameter signature's predictive model for the NSCLC patients with BMs was established according to the results of LASSO-Cox regression analysis. The C-index of the prognostic model to predict OS was 0.672 (95% CI = 0.609 ~ 0.736) which was significantly higher than APA,RPA and GPA. The td-ROC curve and DCA of the predictive model also demonstrated good predictive accuracy of OS compared to APA, RPA and GPA. Moreover, NRI and IDI analysis indicated that the prognostic model had improved prediction ability compared with APA, RPA and GPA.

Conclusion: The novel prognostic model demonstrated favorable performance than APA, RPA, and GPA for predicting OS in NSCLC patients with BMs.
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http://dx.doi.org/10.1186/s12935-022-02671-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367158PMC
August 2022

Establishment and Evaluation of a Porcine Vein Graft Disease Model.

J Vis Exp 2022 07 25(185). Epub 2022 Jul 25.

Department of Cardiovascular Surgery, the First Affiliated Hospital of Jinan University;

Venous graft disease (VGD) is the leading cause of coronary artery bypass graft (CABG) failure. Large animal models of CABG-VGD are needed for the investigation of disease mechanisms and the development of therapeutic strategies. To perform the surgery, we enter the cardiac chamber through the third intercostal space and carefully dissect the internal mammary vein and immerse it in normal saline. The right main coronary artery is then treated for ischemia. The target vessel is incised, a shunt plug is placed, and the distal end of the graft vein is anastomosed. The ascending aorta is partially blocked, and the proximal end of the graft vein is anastomosed after perforation. The graft vein is checked for patency, and the proximal right coronary artery is ligated. CABG surgery is performed in minipigs to harvest the left internal mammary vein for its use as a vascular graft. Serum biochemical tests are used to evaluate the physiological status of the animals after surgery. Ultrasound examination shows that the proximal, middle, and distal end of the graft vessel are unobstructed. In the surgical model, turbulent blood flow in the graft is observed upon histological examination after the CABG surgery, and venous graft stenosis associated with intimal hyperplasia is observed in the graft. The study here provides detailed surgical procedures for the establishment of a repeatable CABG-induced VGD model.
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http://dx.doi.org/10.3791/63896DOI Listing
July 2022

New insight into the sucrose biosynthesis pathway from genome-wide identification, gene expression analysis, and subcellular localization in hexaploid wheat (Triticum aestivum L.).

J Plant Physiol 2022 Jul 16;276:153770. Epub 2022 Jul 16.

Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, 100048, China. Electronic address:

Sucrose, the main synthesized product and transported form of photoassimilates, moves from leaves to support plant growth and storage substance biosynthesis occurring in the heterotrophic sink organs. Enhancing sucrose biosynthesis efficiency is a top priority for crop yield breeding programs. However, the molecular mechanism of sucrose biosynthesis is still elusive, especially in wheat. We performed transcriptome sequencing, subcellular localization, and bioinformatics analysis to identify key isoforms and metabolic branches associated with sucrose biosynthesis in wheat. Our analysis identified 45 genes from 13 families that exhibited high expression in young leaves with an evident diurnal change. The carbon flux from photoassimilates to sucrose was divided into two pathways. In the cytoplasm, assimilates initiating at phosphotrioses (TPs) exported by TaTPT1 from chloroplasts flowed along the TP-Sucrose branch formed by TaALD6, TaFBP5, TacPGI, TacPGM, TaUGP1, TaSPS5, and TaSPP1. Intermediates either from the Calvin cycle or TP-Sucrose branch were converted into ADPGlc to synthesize the simple starch, which was transiently degraded by a series of enzymes, including TaBAM4 and TaSEX4 in the chloroplast. Similar to the starch-biosynthesis branch in endosperms, the TP-Sucrose branch is the most prominent in leaves because each reaction can be catalyzed by at least one highly expressed isoform with expected cytosolic localization. The key isoforms and major branches highlighted in the wheat sucrose biosynthesis pathway expand our molecular understanding of crop sucrose biosynthesis and provide clues to increase wheat yield by enhancing the sucrose synthesis efficiency of leaves.
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http://dx.doi.org/10.1016/j.jplph.2022.153770DOI Listing
July 2022

A multi-layer functional genomic analysis to understand noncoding genetic variation in lipids.

Am J Hum Genet 2022 08;109(8):1366-1387

Division of Genome Science, Department of Precision Medicine, National Institute of Health, Chungbuk, South Korea.

A major challenge of genome-wide association studies (GWASs) is to translate phenotypic associations into biological insights. Here, we integrate a large GWAS on blood lipids involving 1.6 million individuals from five ancestries with a wide array of functional genomic datasets to discover regulatory mechanisms underlying lipid associations. We first prioritize lipid-associated genes with expression quantitative trait locus (eQTL) colocalizations and then add chromatin interaction data to narrow the search for functional genes. Polygenic enrichment analysis across 697 annotations from a host of tissues and cell types confirms the central role of the liver in lipid levels and highlights the selective enrichment of adipose-specific chromatin marks in high-density lipoprotein cholesterol and triglycerides. Overlapping transcription factor (TF) binding sites with lipid-associated loci identifies TFs relevant in lipid biology. In addition, we present an integrative framework to prioritize causal variants at GWAS loci, producing a comprehensive list of candidate causal genes and variants with multiple layers of functional evidence. We highlight two of the prioritized genes, CREBRF and RRBP1, which show convergent evidence across functional datasets supporting their roles in lipid biology.
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http://dx.doi.org/10.1016/j.ajhg.2022.06.012DOI Listing
August 2022

Radioimmunotherapy-induced intratumoral changes in cervical squamous cell carcinoma at single-cell resolution.

Cancer Commun (Lond) 2022 Jul 27. Epub 2022 Jul 27.

Department of Radiation Oncology and Shandong Provincial Key Laboratory of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, 250117, P. R. China.

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http://dx.doi.org/10.1002/cac2.12342DOI Listing
July 2022

Microsatellite Characteristics of Silver Carp () Genome and Genetic Diversity Analysis in Four Cultured Populations.

Genes (Basel) 2022 Jul 16;13(7). Epub 2022 Jul 16.

Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

is one of the four most important fish in China and has high breeding potential. However, simple sequence repeat (SSR) markers developed on genome level for genetic diversity analysis are limited. In this study, the distribution characteristics of SSRs in the assembled genome were analyzed, and new markers were developed to preliminarily evaluate the genetic diversity of the four breeding populations. A total of 368,572 SSRs were identified from the genome. The total length of SSRs was 6,492,076 bp, accounting for 0.77% of the total length of the genome sequence. The total frequency and total density were 437.73 loci/Mb and 7713.16 bp/Mb, respectively. Among the 2-6 different nucleotide repeat types, SSRs were dominated by di-nucleotide repeats (204,873, 55.59%), and AC/GT was the most abundant motif. The number of SSRs on each chromosome was positively correlated with the length. The 13 pairs of markers developed were used to analyze the genetic diversity of four cultivated populations in Hubei Province. The results showed that the genetic diversity of the four populations was low, and the ranges of alleles (Na), effective alleles (Ne), observed heterozygosity (Ho), and Shannon's index information (I) were 3.538-4.462, 2.045-2.461, 0.392-0.450, and 0.879-0.954, respectively. Genetic variation occurs mainly among individuals within populations (95.35%). UPGMA tree and Bayesian analysis showed that four populations could be divided into two different branches. Therefore, the genome-wide SSRs were effectively in genetic diversity analysis on
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http://dx.doi.org/10.3390/genes13071267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320178PMC
July 2022

Realization of English Instructional Resources Clusters Reconstruction System Using the Machine Learning Model.

Authors:
Xiaohui Li

Comput Intell Neurosci 2022 9;2022:2838935. Epub 2022 Jul 9.

School of Foreign Languages, Xuchang University, Xuchang 461000, China.

Based on ML algorithm, this paper puts forward a method that can search instructional resources through keyword indexing technology, and then cluster and recombine the related results and present them centrally. In this paper, the semantic processing of user query based on the subject index of educational resources is adopted to make up for the deficiency of query semantics, solve the problem of mismatch between query words and document words, and improve the recall and precision of resource retrieval. It is proposed to select the category feature items manually and establish the category feature model. In the environment of small sample set, the weight of category feature items is trained by ML method. The research shows that the user rating of this system is ideal, reaching 93.21% at the highest. In addition, the stability of the system can still reach 89.31% under the condition of relatively large usage, and its performance is excellent. This system can effectively solve the problem of scattered distribution of English instructional resources and make the presentation of knowledge more in line with the needs of users, thereby further improving the utilization rate of English instructional resources and users' satisfaction.
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http://dx.doi.org/10.1155/2022/2838935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288350PMC
July 2022

Impact of Infection and Outcome of Anti- Therapy in Patients with Reflux Laryngopharyngitis.

Evid Based Complement Alternat Med 2022 5;2022:8266321. Epub 2022 Jul 5.

Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objectives: This study was designed to explore the relationship between (Hp) infection and reflux laryngopharyngitis (RLP) and to evaluate the outcome of anti-Hp therapy in improving RLP symptoms.

Methods: A total of 410 patients with RLP were enrolled and tested for Hp infection. The association of Hp infection with reflux symptom index (RSI) and reflux finding score (RFS) was determined. Hp-positive patients received either a proton pump inhibitor (PPI) omeprazole alone (control group) or a combination regimen (experimental group) consisting of omeprazole, mosapride citrate, amoxicillin, and clarithromycin. Therapeutic outcomes were compared 4 weeks later.

Results: Of the 410 participants, 290 were Hp-positive and 120 Hp-negative. Both RSI and RFS were significantly higher in Hp-positive patients than in Hp-negative patients. Hp infection status was positively correlated with RSI ( < 0.05) and RFS ( < 0.05). The overall response rate was higher in the experimental group than in the control group. Both the groups had a significant reduction in RSI and RFS after therapy, with a greater improvement in the experimental group ( < 0.05).

Conclusion: Our findings establish a link between Hp infection and RLP. Anti-Hp therapy improves RSI and RFS in RLP patients. Therefore, Hp eradication drugs may be added to the PPI-based regimen in the treatment of RLP.
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http://dx.doi.org/10.1155/2022/8266321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276480PMC
July 2022

Acute Myocarditis Following Methimazole: A Case Report.

Clin Ther 2022 Jul 11. Epub 2022 Jul 11.

Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China. Electronic address:

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http://dx.doi.org/10.1016/j.clinthera.2022.05.006DOI Listing
July 2022

Construction of a Glycaemia-Based Signature for Predicting Acute Kidney Injury in Ischaemic Stroke Patients after Endovascular Treatment.

J Clin Med 2022 Jul 3;11(13). Epub 2022 Jul 3.

Department of Neurology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.

Background: Hyperglycaemia is thought to be connected to worse functional outcomes after ischaemic stroke. However, the association between hyperglycaemia and acute kidney injury (AKI) after endovascular treatment (EVT) remains elusive. The purpose of this study was to investigate the influence of glycaemic on AKI after EVT.

Methods: We retrospectively collected the clinical information of patients who underwent EVT from April 2015 to August 2021. Blood glucose after EVT was recorded as acute glycaemia. Chronic glucose levels were estimated by glycosylated haemoglobin (HbA) using the following formula: chronic glucose levels (mg/dL) = 28.7 × HbA (%) - 46.7. AKI was defined as an increase in maximum serum creatinine to ≥1.5 baseline. We evaluated the association of AKI with blood glucose. A nomogram was established to predict the risk of AKI, and its diagnostic efficiency was determined by decision curve analysis.

Results: We enrolled 717 acute ischaemic stroke patients who underwent EVT. Of them, 205 (28.6%) experienced AKI. Acute glycaemia (OR: 1.007, 95% CI: 1.003-1.011, < 0.001), the acute/chronic glycaemic ratio (OR: 4.455, 95% CI: 2.237-8.871, < 0.001) and the difference between acute and chronic glycaemia (Δ) (OR: 1.008, 95% CI: 1.004-1.013, < 0.001) were associated with the incidence of AKI. Additionally, age, atrial fibrillation, ASITN/SIR collateral grading, postoperative mTICI scale, and admission NIHSS were also significantly correlated with AKI. We then created a glycaemia-based nomogram, and its concordance index was 0.743. The net benefit of the nomogram was further confirmed by decision curve analysis.

Conclusions: The glycaemia-based nomogram may be used to predict AKI in ischaemic stroke patients receiving EVT.
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http://dx.doi.org/10.3390/jcm11133865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267863PMC
July 2022

eIF6 is potential diagnostic and prognostic biomarker that associated with F-FDG PET/CT features and immune signatures in esophageal carcinoma.

J Transl Med 2022 07 6;20(1):303. Epub 2022 Jul 6.

Department of Nuclear Medicine and Institute of Anesthesiology and Pain, and Department of Pathology, Taihe Hospital, Hubei University of Medicine, No. 32, Renmin Road, Shiyan, 442000, China.

Background: Although eukaryotic initiation factor 6 (eIF6) is a novel therapeutic target, data on its importance in the development of esophageal carcinoma (ESCA) remains limited. This study evaluated the correlation between eIF6 expression and metabolic analysis using fluorine-18 fluorodeoxyglucose (F-FDG) -Positron emission tomography (PET) and immune gene signatures in ESCA.

Methods: This study employed The Cancer Genome Atlas (TCGA) to analyze the expression and prognostic value of eIF6, as well as its relationship with the immune gene signatures in ESCA patients. The qRT-PCR and Western blot analyses were used to profile the expression of eIF6 in ESCA tissues and different ESCA cell lines. The expression of tumor eIF6 and glucose transporter 1 (GLUT1) was examined using immunohistochemical tools in fifty-two ESCA patients undergoing routine F-FDG PET/CT before surgery. In addition, the cellular responses to eIF6 knockdown in human ESCA cells were assessed via the MTS, EdU, flow cytometry and wound healing assays.

Results: Our data demonstrated that compared with the normal esophageal tissues, eIF6 expression was upregulated in ESCA tumor tissues and showed a high diagnostic value with an area under curve of 0.825 for predicting ESCA. High eIF6 expression was significantly correlated with shorter overall survival of patients with esophagus adenocarcinoma (p = 0.038), but not in squamous cell carcinoma of the esophagus (p = 0.078). In addition, tumor eIF6 was significantly associated with F-FDG PET/CT parameters: maximal and mean standardized uptake values (SUVmax and SUVmean) and total lesion glycolysis (TLG) (rho = 0.458, 0.460, and 0.300, respectively, p < 0.01) as well as GLUT1 expression (rho = 0.453, p < 0.001). A SUVmax cutoff of 18.2 led to prediction of tumor eIF6 expression with an accuracy of 0.755. Functional analysis studies demonstrated that knockdown of eIF6 inhibited ESCA cell growth and migration, and fueled cell apoptosis. Moreover, the Bulk RNA gene analysis revealed a significant inverse association between eIF6 and the tumor-infiltrating immune cells (macrophages, T cells, or Th1 cells) and immunomodulators in the ESCA microenvironment.

Conclusion: Our study suggested that eIF6 might serve as a potential prognostic biomarker associated with metabolic variability and immune gene signatures in ESCA tumor microenvironment.
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http://dx.doi.org/10.1186/s12967-022-03503-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258187PMC
July 2022

Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins.

Nat Commun 2022 07 6;13(1):3880. Epub 2022 Jul 6.

Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel.

Sexual reproduction consists of genome reduction by meiosis and subsequent gamete fusion. The presence of genes homologous to eukaryotic meiotic genes in archaea and bacteria suggests that DNA repair mechanisms evolved towards meiotic recombination. However, fusogenic proteins resembling those found in gamete fusion in eukaryotes have so far not been found in prokaryotes. Here, we identify archaeal proteins that are homologs of fusexins, a superfamily of fusogens that mediate eukaryotic gamete and somatic cell fusion, as well as virus entry. The crystal structure of a trimeric archaeal fusexin (Fusexin1 or Fsx1) reveals an archetypical fusexin architecture with unique features such as a six-helix bundle and an additional globular domain. Ectopically expressed Fusexin1 can fuse mammalian cells, and this process involves the additional globular domain and a conserved fusion loop. Furthermore, archaeal fusexin genes are found within integrated mobile elements, suggesting potential roles in cell-cell fusion and gene exchange in archaea, as well as different scenarios for the evolutionary history of fusexins.
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http://dx.doi.org/10.1038/s41467-022-31564-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259645PMC
July 2022

MCPIP1 promotes cell proliferation, migration and angiogenesis of glioma via VEGFA-mediated ERK pathway.

Exp Cell Res 2022 Sep 22;418(1):113267. Epub 2022 Jun 22.

Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui, 230032, China. Electronic address:

Glioma is the most common primary malignant intracranial tumor in the population, and is often associated with abundant angiogenesis. However, how angiogenesis is regulated during glioma progression is still poorly understood. Data mining of cancer patient database shows that MCPIP1 is positively correlated with VEGFA expression and negatively with survival. In this study, we report that overexpressed MCPIP1 in glioma cells is a boost of angiogenesis. Mechanistically, MCPIP1 upregulates the expression of VEGFA in glioma, and promote the secretion of VEGFA to the surroundings, which could stimulate angiogenesis through ERK pathway. Blocking VEGFA expression and secretion inhibited MCPIP1-mediated angiogenesis and glioma progression in vitro and xenograft models. Collectively, these results identify a critical role for MCPIP1 in angiogenesis and glioma progression by regulating the VEGFA-mediated ERK pathway, suggesting that targeting MCPIP1 may be a potential glioma-selective therapeutic strategy.
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http://dx.doi.org/10.1016/j.yexcr.2022.113267DOI Listing
September 2022

Double Perovskite BaLaTaO for Ultrafast Fiber Lasers in Anomalous and Normal Net Dispersion Regime.

Nanomaterials (Basel) 2022 Jun 20;12(12). Epub 2022 Jun 20.

School of Computer Science and Engineering, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macao, China.

Double perovskites (DPs) have been attracting attention in an assortment of optoelectronic applications, for they hold advantages such as high quantum efficiency, long carrier migration distance and strong linear and nonlinear absorptions. As specific kinds of perovskites (PVKs), DPs are gifted with orthorhombic crystal structures which provide rich conversion combinations and broaden the space for research and application. However, few works have been reported about DPs in ultrafast photonics applications. In this article, a DP with chemical formula of BaLaTaO (BLT) was successfully synthesized by high-temperature solid phase method. The microstructures and morphologies were observed, and the linear and nonlinear absorption were characterized. By first using BLT as a novel saturable absorber in both normal and anomalous dispersion region fiber lasers, dual-wavelength soliton and dissipative soliton were successfully operated at C-band. This study affirms BLT's nonlinear optical properties, lays the foundation for optical research on BLT, and meanwhile provides a meaningful reference for future development of pulsed lasers based on DPs.
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http://dx.doi.org/10.3390/nano12122112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229686PMC
June 2022

Factors Affecting the Exercise Capacity in Pediatric Primary Hypertension.

Front Pediatr 2022 25;10:882223. Epub 2022 May 25.

Department of Cardiovascular Medicine, Children's Hospital Capital Institute of Pediatrics, Beijing, China.

Purpose: Exercise training is crucial to the early intervention of pediatric primary hypertension (PHT). However, much less is known about exercise capacity in this disease. This work investigated the exercise capacity in pediatric PHT and analyzed the factors affecting exercise capacity.

Methods: The study enrolled children with PHT at the Children's Hospital Capital Institute of Pediatrics between July 2017 and July 2020. The Bruce protocol of the treadmill exercise test (TET) was used to assess exercise capacity. Multivariate ordinal logistic regression and generalized linear models were used to analyze factors affecting exercise capacity.

Results: Of 190 patients, 146 (76.8%) were male, and the median age was 13 (11, 14). Most children accomplished TET and achieved the submaximal heart rates (189 [99.5%]). Children with lower resting diastolic blood pressure (DBP) and 24 h average diastolic blood pressure (ADBP) could achieve a TET stage of 6 or more, whereas children with higher DBP and ADBP could only achieve a TET stage of 3 ( all < 0.05). Children with lower DBP and 24 h ADBP were also associated with greater metabolic equivalents (METs; = -0.237, = -0.179, all < 0.05). The completion of TET stages was negatively associated with female (OR = 0.163), younger age (OR = 1.198), greater body mass index (BMI, OR = 0.921), and higher 24 h ADBP (OR = 0.952, all < 0.05). In addition, METs were negatively associated with female (β = -1.909), younger age (β = 0.282), greater BMI (β = -0.134), and higher 24 h ADBP (β = -0.063, all < 0.05).

Conclusions: Exercise capacity was impaired among pediatric PHT patients. Female gender, younger age, greater BMI, and higher 24 h ADBP are independently associated with the exercise capacity in pediatric PHT. These findings may help developing scientific exercise prescriptions for pediatric PHT.
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http://dx.doi.org/10.3389/fped.2022.882223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174901PMC
May 2022

TPN171H alleviates pulmonary hypertension via inhibiting inflammation in hypoxia and monocrotaline-induced rats.

Vascul Pharmacol 2022 Jun 6;145:107017. Epub 2022 Jun 6.

Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, Hunan, China. Electronic address:

Pulmonary hypertension (PH) is a progressive and life-threatening disease with poor prognosis despite many advances in medical therapy over the past 20 years. Novel therapies which target on the underlying pathology of PH are still urgent to be met. TPN171H is a recently found new compound that exhibits potent pharmacological effects in PH via inhibiting phosphodiesterase type 5 (PDE-5). However, as one icariin derivative, the anti-inflammatory effects of TPN171H for treating PH are not clear. The present study was designed to investigate the therapeutical effect of TPN171H against inflammation in PH and reveal the underlying mechanism. Hypoxia and monocrotaline (MCT)-induced PH rat models were established, which were treated by oral administration of TPN171H (5, 25 mg/kg/d) or sildenafil (25 mg/kg/d). The right ventricle systolic pressure (RVSP), right ventricle hypertrophy index (RVHI) and vascular remodeling were measured. The results suggested that TPN171H significantly reduced RVSP and RVHI, and reversed pulmonary vascular remodeling in rats with both models. Furthermore, in in vivo and in vitro research, our data suggested that TPN171H remarkably suppressed cathepsin B-mediated NLRP3 inflammasome activation, which may contribute to its therapeutical function for PH.
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http://dx.doi.org/10.1016/j.vph.2022.107017DOI Listing
June 2022

Editorial: Progresses in the Drug Treatment of Chronic Cardiopulmonary Diseases.

Front Pharmacol 2022 19;13:910212. Epub 2022 May 19.

Department of Cardiology, Gansu Provincial Hospital, Lanzhou, China.

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http://dx.doi.org/10.3389/fphar.2022.910212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160424PMC
May 2022

A multiancestry genome-wide association study of unexplained chronic ALT elevation as a proxy for nonalcoholic fatty liver disease with histological and radiological validation.

Nat Genet 2022 06 2;54(6):761-771. Epub 2022 Jun 2.

Department of Genetics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.

Nonalcoholic fatty liver disease (NAFLD) is a growing cause of chronic liver disease. Using a proxy NAFLD definition of chronic elevation of alanine aminotransferase (cALT) levels without other liver diseases, we performed a multiancestry genome-wide association study (GWAS) in the Million Veteran Program (MVP) including 90,408 cALT cases and 128,187 controls. Seventy-seven loci exceeded genome-wide significance, including 25 without prior NAFLD or alanine aminotransferase associations, with one additional locus identified in European American-only and two in African American-only analyses (P < 5 × 10). External replication in histology-defined NAFLD cohorts (7,397 cases and 56,785 controls) or radiologic imaging cohorts (n = 44,289) replicated 17 single-nucleotide polymorphisms (SNPs) (P < 6.5 × 10), of which 9 were new (TRIB1, PPARG, MTTP, SERPINA1, FTO, IL1RN, COBLL1, APOH and IFI30). Pleiotropy analysis showed that 61 of 77 multiancestry and all 17 replicated SNPs were jointly associated with metabolic and/or inflammatory traits, revealing a complex model of genetic architecture. Our approach integrating cALT, histology and imaging reveals new insights into genetic liability to NAFLD.
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http://dx.doi.org/10.1038/s41588-022-01078-zDOI Listing
June 2022

Identification and Validation of Serum CST1 as a Diagnostic Marker for Differentiating Early-Stage Non-Small Cell Lung Cancer from Pulmonary Benign Nodules.

Cancer Control 2022 Jan-Dec;29:10732748221104661

Department of Clinical Laboratory, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, 71067Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: Effective means for early diagnosis are imperative to reduce death rate of non-small cell lung cancer (NSCLC) patients. We aimed to find out high-performance serologic markers to distinguish early-stage NSCLC patients from benign pulmonary nodule patients and healthy controls (HC). Cystatin-SN (CST1) is an active cysteine protease inhibitor of the CST superfamily, involving in the processes of inflammation and tumorigenesis. This is the first exploration of the diagnostic and prognostic values of serum CST1 in NSCLC.

Methods: We analyzed the transcriptome data from The Cancer Genome Atlas and the Gene Expression Omnibus database, screened biomarkers for NSCLC, and verified the candidate markers via the ONCOMINE database. Then, we performed ELISA, western blotting, and immunohistochemistry analysis to detect the expression levels of CST1 in NSCLC cell lines, tumor tissues, and serum samples of clinical cohorts.

Results: We identified 3 up-regulated secreted protein-encoding genes, validated the expression levels of CST1 in NSCLC tumor tissues and cell lines, and found that serum CST1 levels of NSCLC (4289 ± 2405 pg/mL) were significantly higher than those of PBN patients (1558 ± 441 pg/mL, < .0001) and healthy controls (1529 ± 416 pg/mL, < .0001). The AUC of the combination of CST1, Cytokeratin 19 fragment (Cyfra21-1), and Carcinoembryonic antigen (CEA) for distinguishing early-stage NSCLC from PBN/HC was as high as .914/0.925. Furthermore, our results suggested that the NSCLC patient with low serum CST1 level had a better survival rate.

Conclusions: Serum CST1 may serve as a novel diagnostic marker for differentiating early-stage NSCLC from PBN and HC, and could be used as a prognosis predictor in NSCLC patients.
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http://dx.doi.org/10.1177/10732748221104661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168853PMC
June 2022

HTPdb and HTPtools: Exploiting maize haplotype-tag polymorphisms for germplasm resource analyses and genomics-informed breeding.

Plant Commun 2022 Jul 5;3(4):100331. Epub 2022 May 5.

Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences (BAAFS), Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Shuguang Garden Middle Road No. 9, Beijing 100097, China. Electronic address:

Along with rapid advances in high-throughput-sequencing technology, the development and application of molecular markers has been critical for the progress that has been made in crop breeding and genetic research. Desirable molecular markers should be able to rapidly genotype tens of thousands of breeding accessions with tens to hundreds of markers. In this study, we developed a multiplex molecular marker, the haplotype-tag polymorphism (HTP), that integrates Maize6H-60K array data from 3,587 maize inbred lines with 6,375 blocks from the recombination block map. After applying strict filtering criteria, we obtained 6,163 highly polymorphic HTPs, which were evenly distributed in the genome. Furthermore, we developed a genome-wide HTP analysis toolkit, HTPtools, which we used to establish an HTP database (HTPdb) covering the whole genomes of 3,587 maize inbred lines commonly used in breeding. A total of 172,921 non-redundant HTP allelic variations were obtained. Three major HTPtools modules combine seven algorithms (e.g., chain Bayes probability and the heterotic-pattern prediction algorithm) and a new plotting engine named "BCplot" that enables rapid visualization of the background information of multiple backcross groups. HTPtools was designed for big-data analyses such as complex pedigree reconstruction and maize heterotic-pattern prediction. The HTP-based analytical strategy and the toolkit developed in this study are applicable for high-throughput genotyping and for genetic mapping, germplasm resource analyses, and genomics-informed breeding in maize.
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http://dx.doi.org/10.1016/j.xplc.2022.100331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284292PMC
July 2022

Dysbiosis of gut microbiota and intestinal damage in mice induced by a single intravenous exposure to CdTe quantum dots at low concentration.

J Appl Toxicol 2022 May 26. Epub 2022 May 26.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing, China.

Although quantum dots (QDs) have shown great potential for various biomedical applications, their potential toxicity still needs to be comprehensively investigated. Previous studies showed that intravenous exposure of CdTe QDs at low concentration did not lead to obvious in vivo toxicity in the long term. However, the influence of CdTe QDs on the gut microbiota and the intestine is still unknown. Here, we explored whether single intravenous injection of CdTe QDs at low concentration can affect the gut microbiota and intestine of mice in short term. The results showed that CdTe QDs caused an imbalance of gut microbiota, especially the rapid increase in Lactobacillus on day 1 post-treatment. Meanwhile, the intestine exhibited the promotion of oxidative stress, inflammatory response, and hemorrhaging on days 5 and 15. These results demonstrate that the gut microbiota and the intestine are very sensitive to the toxicity of low-concentration CdTe QDs. This study provides further insight and method for the biosafety evaluation of nanomaterials.
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http://dx.doi.org/10.1002/jat.4352DOI Listing
May 2022

Temporal variation of the coupling relationship between methanogens and biogeochemical process in soil-microbes-rice system.

Chemosphere 2022 Sep 23;303(Pt 1):135099. Epub 2022 May 23.

Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province/Center for Eco-Environmental Restoration Engineering of Hainan Province/College of Ecology & Environment/State Key Laboratory of Marine Resource Utilization in South China Sea/Key Laboratory for Environmental Toxicology of Haikou, Haikou, Hainan University, Hainan, 570228, China.

Microbial community were most resilient option for methane associated mitigation strategies. Biogas slurry provides plant nutrition and affects microbial community. However, little is known about the changes of the functional guilds (methanogen and methanotroph) in the geochemical context after addition biogas slurry. For this purpose, a pot experiment was conducted. Six treatment groups were included in this study, four with biogas slurry: water ratio (1:4, T02; 2:3, T04; 3:2, T06; 4:1, T08), one with a chemical fertilizer (F), and a control (CK). The effective tiller and biomass significantly increased by 1.9 times and 2.1 times in T02 relative to CK. The relative abundance of Bacteroidetes in the biogas slurry treatments was 31.5%, while that in CK was 11.4%. The dominant methanogens in CK, F and treatments were different at heading and mature stages. CK and F were hydrogenotrophs with relative abundance of 0.09% and 0.06%, and the treatment group was acetotrophs with mean value of 1.21% at heading stage. Compared with CK, the number of methanotrophs in the treatments at heading stage increased by 4.1 times, while that at mature increased by 10.3 times. The methanogenic community in the treatments may be shaped by the amount of biogas slurry applied rather than by biogeochemical processes at heading stage. Nevertheless, there may be existed synergistic interaction in the soil-microbes-rice system at mature stage. These findings may provide a better understanding of regulating soil respiration in agricultural land.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135099DOI Listing
September 2022

Incidence of accidental falls and development of a fall risk prediction model among elderly patients with diabetes mellitus: A prospective cohort study.

J Clin Nurs 2022 May 20. Epub 2022 May 20.

NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China.

Aims: To investigate the incidence of accidental falls and develop a fall risk prediction tool in elderly patients with diabetes mellitus.

Background: The risk of fall in elderly patients with diabetes is higher than that in the general elderly, there is fewer fall assessment tools for elderly patients with diabetes.

Design: A prospective cohort study.

Methods: Between June and September 2019, a total of 1007 elderly patients with diabetes were enrolled from a tertiary specialist diabetes hospital in Tianjin and were prospectively followed up for 6 months to determine outcomes of accidental falls through telephone. Demographic and diseases related factors were collected at baseline. Incidence of falls was investigated, and a nomogram was developed based on logistic regression model. SPSS 21.0 and R 3.6.3 were used to analyse the data. The article was reported in accordance with STROBE guidelines.

Results: Among 1007 elderly patients, 950 finished the follow-up. A total of 133 falls occurred in 93 patients during the follow-up period, with a fall rate of 9.79%. Diabetic peripheral neuropathy, walking aids, depression, fall history, fatigue and sex were independent predictors of accidental fall in diabetes elderly patients. The sensitivity and specificity of the predictive model were 73.12% and 52.63%, respectively, and a fall risk prediction nomogram was developed based on the regression model.

Conclusions: A nomogram including 6 easily available prediction factors (diabetic peripheral neuropathy, walking aids, depression, fall history within 1 year, fatigue, sex) was developed, and it can be used in safety management among Chinese elderly patients diagnosed with diabetes.

Relevance To Clinical Practice: Nomogram can be used to identify diabetic elderly patients at high risk of accidental falls.
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http://dx.doi.org/10.1111/jocn.16371DOI Listing
May 2022

The mucin-like, secretory type-I transmembrane glycoprotein GP900 in the apicomplexan Cryptosporidium parvum is cleaved in the secretory pathway and likely plays a lubrication role.

Parasit Vectors 2022 May 17;15(1):170. Epub 2022 May 17.

Key Laboratory of Zoonosis Research of the Ministry of Education, The Institute of Zoonosis, The College of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Background: Cryptosporidium parvum is a zoonotic parasite and member of the phylum Apicomplexa with unique secretory organelles, including a rhoptry, micronemes and dense granules that discharge their contents during parasite invasion. The mucin-like glycoprotein GP900 with a single transmembrane domain is an immunodominant antigen and micronemal protein. It is relocated to the surface of excysted sporozoites and shed to form trails by sporozoites exhibiting gliding motility (gliding sporozoites). However, the biological process underlying its relocation and shedding remains unclear. The primary aim of this study was to determine whether GP900 is present as a transmembrane protein anchored to the plasma membrane on the surface of sporozoites and whether it is cleaved before being shed from the sporozoites.

Methods: Two anti-GP900 antibodies, a mouse monoclonal antibody (mAb) to the long N-terminal domain (GP900-N) and a rabbit polyclonal antibody (pAb) to the short C-terminal domain (GP900-C), were produced for the detection of intact and cleaved GP900 proteins in sporozoites and other parasite developmental stages by microscopic immunofluorescence assay and in discharged molecules by enzyme-linked immunosorbent assay.

Results: Both anti-GP900 antibodies recognized the apical region of unexcysted and excysted sporozoites. However, anti-GP900-N (but not anti-GP900-C) also stained both the pellicles/surface of excysted sporozoites and the trails of gliding sporozoites. Both antibodies stained the intracellular meronts, both developing and developed, but not the macro- and microgamonts. Additionally, the epitope was recognized by anti-GP900-N (but not anti-GP900-C) and detected in the secretions of excysted sporozoites and intracellular parasites.

Conclusions: GP900 is present in sporozoites and intracellular meronts, but absent in sexual stages. It is stored in the micronemes of sporozoites, but enters the secretory pathway during excystation and invasion. The short cytoplasmic domain of GP900 is cleaved in the secretory pathway before it reaches the extracellular space. The molecular features and behavior of GP900 imply that it plays mainly a lubrication role.
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http://dx.doi.org/10.1186/s13071-022-05286-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111948PMC
May 2022

Phillyrin attenuates norepinephrine-induced cardiac hypertrophy and inflammatory response by suppressing p38/ERK1/2 MAPK and AKT/NF-kappaB pathways.

Eur J Pharmacol 2022 Jul 13;927:175022. Epub 2022 May 13.

Institute of Materia Medica and Department of Pharmaceutics, College of Pharmacy, Army Medical University, Chongqing, 400038, China; Chongqing Engineering Research Center for Pharmacodynamics Evaluation, College of Pharmacy, Army Medical University, Chongqing, 400038, China. Electronic address:

Phillyrin, a well-known natural compound from the dried fruits of Forsythia suspensa (Thunb.) Vahl., has shown anti-inflammatory, antioxidant and anti-virus activities as well as renal protective effects on diabetic nephropathy. In this study, we investigated whether phillyrin attenuated cardiac hypertrophy induced by catecholamine in vivo and in vitro, and explored the underlying mechanisms. Cardiac hypertrophy was induced in C57BL/6 mice by subcutaneous injection of norepinephrine (NE, a key catecholamine), and in rat cardiomyoblasts (H9c2) by stimulation with NE in vitro. Our results showed that administration of phillyrin (100 mg/kg, i.p. for 15 days) significantly improved cardiac function, histopathological changes, cardiac hypertrophy and decreased the upregulated hypertrophic markers (ANP, BNP, and β-MHC). Moreover, treatment with phillyrin obviously reduced the infiltration of the CD68 positive macrophages and the mRNA expression of proinflammatory genes (IL-1β, IL-6, and TNF-α) in left ventricular tissue. In addition, treatment with phillyrin markedly inhibited the phosphorylation of p38 MAPK, ERK1/2, AKT, and NF-κB p65 in heart tissues. Furthermore, in NE-treated H9c2 cells, pretreatment with phillyrin clearly attenuated cardiomyocyte hypertrophy, reduced ROS production and inhibited the phosphorylation of p38 MAPK, ERK1/2, AKT, and NF-κB p65 in vitro. Collectively, our results demonstrate that phillyrin effectively alleviates NE-induced cardiac hypertrophy and inflammatory response by suppressing p38 MAPK/ERK1/2 and AKT/NF-κB signaling pathways.
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http://dx.doi.org/10.1016/j.ejphar.2022.175022DOI Listing
July 2022
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