Publications by authors named "Xiaohu Xu"

31 Publications

Human reliability analysis of high-temperature molten metal operation based on fuzzy CREAM and Bayesian network.

PLoS One 2021 2;16(8):e0254861. Epub 2021 Aug 2.

School of Resources and Civil Engineering, Northeastern University, Shenyang, PR China.

Human errors are considered to be the main causation factors of high-temperature molten metal accidents in metallurgical enterprises. The complex working environment of high- temperature molten metal in metallurgical enterprises has an important influence on the reliability of human behavior. A review of current human reliability techniques confirms that there is a lack of quantitative analysis of human errors in high-temperature molten metal operating environments. In this paper, a model was proposed to support the human reliability analysis of high-temperature molten metal operation in the metallurgy industry based on cognitive reliability and error analysis method (CREAM), fuzzy logic theory, and Bayesian network (BN). The comprehensive rules of common performance conditions in conventional CREAM approach were provided to evaluate various conditions for high-temperature molten metal operation in the metallurgy industry. This study adopted fuzzy CREAM to consider the uncertainties and used the BN to determine the control mode and calculate human error probability (HEP). The HEP for workers involved in high-temperature melting in steelmaking production process was calculated in a case with 13 operators being engaged in different high-temperature molten metal operations. The human error probability of two operators with different control modes was compared with the calculation result of basic CREAM, and the result showed that the method proposed in this paper is validated. This paper quantified point values of human error probability in high-temperature molten metal operation for the first time, which can be used as input in the risk evaluation of metallurgical industry.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254861PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328327PMC
August 2021

Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.

Phytomedicine 2021 Oct 12;91:153654. Epub 2021 Jul 12.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: Polycystic ovary syndrome (PCOS) is a clinical syndrome with reproductive and endocrine disorders. Berberine is a monomer from Chinese herbs such as Coptis chinensis, whose effect on improving ovulation and endometrial receptivity of PCOS is uncertain.

Purpose: To evaluate the effect of berberine on improving PCOS and explore the mechanism.

Methods: The rat model of PCOS was induced by intraperitoneal injection of testosterone propionate. Then they was divided into model (Mod) group, low-dose of berberine (BL) group, high-dose of berberine (BH) group and metformin (Met) group as well as a control (Con) group was established. Ovary morphology, hormone level, glucolipid metabolism were measured. UID-mRNA-seq of ovary tissue was conducted to seek the mechanism of berberine on improving ovulation. Three biomarkers of endometrial receptivity were also examined in endometrium by immunohistochemistry.

Results: The number of cystic follicles was increased while the number of corpus luteum was decreased in the rats of Mod group. These changes could be reversed by high-dose of berberine intervention. Berberine could also decrease the levels of serum luteinizing hormone (LH) and total cholesterol (TC) in PCOS rats. Meanwhile, berberine improved the impairment of abnormal oral glucose tolerance without affecting fasting insulin level and Homeostasis model assessment-insulin resistance (HOMA-IR). Luteinizing hormone/ choriogonadotropin receptor (LHCGR) and cytochrome P450 Family 19 Subfamily A Member 1 (CYP19A1) were focused via RNA-seq of ovary. Protein expression in ovary and mRNA expression in granulosa cell of LHCGR and CYP19A1 were decreased in Mod group and rescued by the intervention of berberine. A decrease of endometrial thickness and an increase of integrin αvβ3 and lysophosphatidic acid receptor 3 (LPAR3) protein expression were observed in Mod group, which could be also reversed by berberbine.

Conclusions: Berberine could improve ovulation in PCOS and the mechanism might be associated with up-regulating LHCGR and CYP19A1. Berberine could also improve endometrial receptivity through down-regualting αvβ3 and LPAR3.
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http://dx.doi.org/10.1016/j.phymed.2021.153654DOI Listing
October 2021

LncRNA LHFPL3-AS1 Promotes Oral Squamous Cell Carcinoma Growth and Cisplatin Resistance Through Targeting miR-362-5p/CHSY1 Pathway.

Onco Targets Ther 2021 31;14:2293-2300. Epub 2021 Mar 31.

Department of Stomatology, Shenzhen Longhua District Central Hospital, Shenzhen, 518000, People's Republic of China.

Background: Oral squamous cell carcinoma (OSCC) is a common oral cancer. The current study aims to elucidate the potential roles of long noncoding RNA (lncRNA) LHFPL3-AS1 in OSCC development.

Methods: Gene expression was measured by qRT-PCR in tumor tissues and cell lines. Loss-of-function assays were performed to analyze the effects of LHFPL3-AS1 on malignant behaviors. Bioinformatics analysis was conducted to explore the downstream signaling pathway of LHFPL3-AS1 in OSCC.

Results: LHFPL3-AS1 was highly expressed in OSCC tissues and cell lines. LHFPL3-AS1 was upregulated in cisplatin-resistant tumor cell lines. LHFPL3-AS1 level was correlated with survival rate. LHFPL3-AS1 knockdown suppressed OSCC proliferation, migration and invasion. LHFPL3-AS1 downregulation reduced cisplatin resistance of OSCC cells. LHFPL3-AS1 was the competing endogenous RNA (ceRNA) for miR-194-5p to enhance CHSY1 expression.

Conclusion: LHFPL3-AS1/miR-362-5p/CHSY1 signaling pathway plays essential roles in regulating OSCC development and cisplatin resistance.
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http://dx.doi.org/10.2147/OTT.S298679DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020056PMC
March 2021

On the phase fading effect in the dual-pulse heterodyne demodulated distributed acoustic sensing system.

Opt Express 2020 Oct;28(22):33433-33447

Understanding signal fading effect is essential for the application of Rayleigh-scattering-based distributed acoustic fibre sensors (DASs) due to the nature of coherent beam interference within the pulse length. Statistical properties for the intensity of the Rayleigh backscattered light (i.e. intensity fading) and its impact on the sensitivity of DAS systems have been intensely studied over the last decades. Here we for the first time establish an analytical model for the phase signal retrieved from the dual-pulse heterodyne demodulated DAS system, which can be exploited to investigate the phase fading effect in this system. The developed model reveals that the phase fading phenomenon mainly originates from the randomness in the phase retardant of the Rayleigh scatters. The quantitatively resolved statistical features of the phase fading is confirmed by experimental results. Based on the analytical model, a noise figure is defined to characterize the global fading-induced noise level via taking into account contributions from all channels along the sensing fiber. The model also reproduces the anti-correlation relation between the power spectrum density of retrieved phase at the heterodyne frequency and the phase fading noise level. Following the analysis and the definition of the noise figure, an optimized real-time weighted-channel stack algorithm is developed to efficiently suppress the fading noise. Experimental results show that the algorithm can achieve a maximum noise figure reduction of 15.8 dB without increasing the system complexity.
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http://dx.doi.org/10.1364/OE.403263DOI Listing
October 2020

General Issues in Clinical Research of Acupuncture and In Vitro Fertilization.

Evid Based Complement Alternat Med 2020 13;2020:3460641. Epub 2020 Apr 13.

Department of Neurology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1095 Jiefang Avenue, Wuhan 430030, China.

In vitro fertilization-embryo transfer (IVF-ET), a well-developed technology, provides the last possibility or hope for infertile women. However, the live birth rate per IVF cycle is still not satisfactory. Acupuncture is a frequently used adjunctive therapy drawing wide attention on improvement of IVF. Although so many randomized controlled trials have been evaluating the effect of acupuncture on IVF in the past 20 years, the exact efficacy of acupuncture on IVF is still controversy mainly because of no consensus on placebo control and acupuncture scheme. This paper focused on the general issues in clinical research of acupuncture and IVF and gave some suggestions for future directions.
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http://dx.doi.org/10.1155/2020/3460641DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174969PMC
April 2020

[Design, screening and antimicrobial activity of novel peptides against ].

Nan Fang Yi Ke Da Xue Xue Bao 2019 Jul;39(7):823-829

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To construct antimicrobial peptides with potent antimicrobial activity, low cytotoxicity and efficient killing rate of for prevention and treatment of dental caries.

Methods: We exploited the existing design strategies to modify reutericin 6 or gassericin A produced by species in the oral cavity based on their cationicity, amphipathicity and -helical structure. We examined their antimicrobial activities using bacterial susceptibility assay, their cytotoxicity through cytotoxicity assay and their killing rate of with time-kill assay. We further evaluated the candidate derivatives for their killing rate against , their antimicrobial activity against different oral pathogens and the development of drug resistance.

Results: We constructed 6 AT-1 derivatives, among which AT-7 showed an MIC of 3.3 μmol/L against , and with a killing rate of 88.7% against within 5 min. We did not obtain strains of resistant to AT- 7 after induction for 10 passages.

Conclusions: Hydrophobicity and imperfect amphipathic structure are two key parameters that define the antimicrobial potency of the antimicrobial peptides. The imperfectly amphipathic peptide AT-7 shows the potential for clinical application in dental caries treatment.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2019.07.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6765563PMC
July 2019

Rational design of peptides with enhanced antimicrobial and anti-biofilm activities against cariogenic bacterium Streptococcus mutans.

Chem Biol Drug Des 2019 10 10;94(4):1768-1781. Epub 2019 Jul 10.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Streptococcus mutans (S. mutans) is known to be a leading cariogenic pathogen in the oral cavity. Antimicrobial peptides possess excellent properties to combat such pathogens. In this study, we compared the antimicrobial activity of novel linear reutericin 6- and/or gassericin A-inspired peptides and identified LR-10 as the leading peptide. Antibacterial assays demonstrate that LR-10 is more active against S. mutans (3.3 μM) than many peptide-based agents without resistance selection, capable of killing many oral pathogens, and tolerant of physiological conditions. LR-10 also presented a faster killing rate than chlorhexidine and erythromycin, and appeared to display selective activity against S. mutans within 10 s. S. mutans is usually encased in plaque biofilms. Biofilm inhibitory assays indicated that LR-10 had excellent inhibitory effect on the biofilm formation of S. mutans and biofilm-encased cells in vitro at low concentrations (6.5 μM). Consistent with most peptides, LR-10 kills S. mutans mainly by disrupting the cell membranes. Notably, both hemolytic activity assays and cytotoxicity tests indicated that LR-10 could keep biocompatible at the effective concentrations. Hence, LR-10 could be a good candidate for clinical treatment of dental caries.
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http://dx.doi.org/10.1111/cbdd.13579DOI Listing
October 2019

Establishment and Comparison of Juvenile Female Mouse Models of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis.

Gastroenterol Res Pract 2018 7;2018:8929620. Epub 2018 Aug 7.

Center of Experimental Animals, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Experimental research has successfully established an adult offspring animal model of nonalcoholic fatty liver disease (NAFLD), but the female offspring model of NAFLD in young age has not been well characterized yet. The aim of this study was to present a direct comparison of the maternal versus postweaning female juvenile NAFLD and nonalcoholic steatohepatitis (NASH) animal models. Four different female mouse models were established and compared using different high-fat diet feeding (HF) strategies in maternal mice and their offspring. The models were non-HF maternal mice and HF offspring with high-high fat (C/HHF), non-HF maternal mice and HF offspring with low-high fat (C/LHF), HF maternal mice and offspring both with high-high fat (HHF/HHF), and HF maternal mice and offspring both with low-high fat (LHF/LHF). A female control group (C/C) was also established. The offspring mice were raised to the age of 8 weeks and then euthanized. Blood glucose levels, lipid profiles, liver function, and triglycerides/total cholesterol contents were examined. Hepatic morphology and superoxide anion levels were evaluated. The nicotinamide-adenine dinucleotide phosphate activity and related regulatory subunits protein expression in the liver tissue were also determined. Our data demonstrated that offspring fat intake contributed to the successful establishment of NAFLD and maternal-offspring fat intake contributed to the successful establishment of NASH in juvenile female mice. Offspring high-fat exposure might be associated with the development of NAFLD and maternal high-fat exposure might be associated with the development of NASH in juvenile female offspring. Higher calories from a fat diet program (both in maternal and offspring) are more prone to inducing liver injury in offspring. In addition, the combination of the aforementioned two factors could aggravate this process. Moreover, oxidative stress was prominent in the juvenile female mouse model of NAFLD/NASH, and the mechanism might be related to the activation of liver NADPH oxidase.
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http://dx.doi.org/10.1155/2018/8929620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6109512PMC
August 2018

Changes of serum trace elements in early stage trauma and its correlation with injury severity score.

Medicine (Baltimore) 2018 Jun;97(23):e10077

The Second Affiliated Hospital of Shantou University Medical College Department of Forensic Medicine, Shantou University Medical College, Shantou, Guangdong Province, People's Republic of China.

Background: Severe trauma can cause secondary multiple organ dysfunction syndrome and death. The absolute and relative concentrations of trace elements in both critical care and conventional treatment, which can lead to acute trace element deficiency, constitute an important mechanism of multiple organ dysfunction syndrome (MODS)/multiple organ failure (MOF).

Methods: We investigated the changes in serum Cu, Zn, and Fe in early stage trauma of patients with the high injury severity score (ISS) and correlated the change in trace elements with ISS. Blood samples were collected within an hour of admittance and the patients were scored according to ISS. We collected clinical data records and ISS score values, and determined serum Fe, Zn, and Cu by inductively coupled plasma mass spectrometry.

Results: Compared with the control group, the serum Zn and Fe values of trauma patients were decreased. There was no significant difference in serum Cu between the patients and the control group. In the trauma group, the serum Zn and Fe were lower than that of the minor injury group, and the difference of Cu concentration was not statistically significant.

Conclusions: Serum Zn and Fe levels in patients with multiple trauma fractures were significantly different than those in the normal group, suggesting that Zn and Fe need to be monitored in the early stage of trauma.
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http://dx.doi.org/10.1097/MD.0000000000010077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5999505PMC
June 2018

Electroacupuncture for Functional Constipation: A Multicenter, Randomized, Control Trial.

Evid Based Complement Alternat Med 2017 31;2017:1428943. Epub 2017 Jan 31.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, China.

. To investigate the efficacy and safety of electroacupuncture (EA) with different current intensities for functional constipation (FC) and to assess whether the effects of EA with different current intensities are superior to the mosapride. . Patients with FC were randomly divided into low current intensity group (LCI), high current intensity group (HCI), and mosapride group (MC). The primary outcome was three or more spontaneous bowel movements (SBMs) per week and an increase of one or more SBMs from baseline during at least 3 of the 4 weeks. . The primary outcome was reached by 53.45%, 66.15%, and 52.24% of the patients who received LCI, HCI, and mosapride, respectively. EA can significantly improve the weekly SBMs and stool consistency and reduce straining severity ( < 0.0001, all). HCI improved the quality of life better than mosapride ( < 0.05) and reduced the proportion of severe constipation more than LCI and mosapride ( < 0.05, both). . EA is effective and safe at both current intensities for FC; therapeutic effects of LCI and HCI are not superior to mosapride. EA is superior to mosapride in improving patients' life quality and satisfaction level of treatment; EA has fewer adverse events than mosapride.
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http://dx.doi.org/10.1155/2017/1428943DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307003PMC
January 2017

Anchoring SbO Nanocrystals on Graphene Sheets for Enhanced Lithium Storage.

ACS Appl Mater Interfaces 2016 Dec 16;8(51):35398-35406. Epub 2016 Dec 16.

Key Laboratory of Eco-Environment-Related Polymer Materials of Ministry of Education, Key Laboratory of Polymer Materials of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University , Lanzhou 730070, Gansu Province, People's Republic of China.

SbO/reduced graphene oxide (SbO/rGO) nanocomposite was synthesized by the solvothermal method using SbO and graphene oxide as raw material. On the basis of the physical and electrochemical characterizations, SbO nanocrystals of 10-20 nm size were uniformly anchored on rGO sheets, and the nanocomposite displayed a large reversible specific capacity of 1271 mA h g and an excellent cyclability of 1090 mA h g after 140 cycles at 100 mA g when proposed as a potential anode material for lithium ion batteries, emphasizing the advantages of anchoring of SbO nanocrystals on rGO sheets for the maximum utilization of electrochemically active SbO and rGO for lithium storage.
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http://dx.doi.org/10.1021/acsami.6b13548DOI Listing
December 2016

Altered Levels of Zinc and N-methyl-D-aspartic Acid Receptor Underlying Multiple Organ Dysfunctions After Severe Trauma.

Med Sci Monit 2015 Sep 3;21:2613-20. Epub 2015 Sep 3.

Department of Pediatric surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China (mainland).

Background: Severe trauma can cause secondary multiple organ dysfunction syndrome (MODS) and death. Oxidative stress and/or excitatory neurotoxicity are considered as the final common pathway in nerve cell injuries. Zinc is the cofactor of the redox enzyme, and the effect of the excitatory neurotoxicity is related to N-methyl-D-aspartic acid receptor (NMDAR).

Material And Methods: We investigated the levels of zinc and brainstem NMDAR in a rabbit model of severe trauma. Zinc and serum biochemical profiles were determined. Immunohistochemistry was used to detect brainstem N-methyl-D-aspartic acid receptor 1 (NR1), N-methyl-D-aspartic acid receptor 2A (NR2A), and N-methyl-D-aspartic acid receptor 2B (NR2B) expression.

Results: Brain and brainstem Zn levels increased at 12 h, but serum Zn decreased dramatically after the trauma. NR1 in the brainstem dorsal regions increased at 6 h after injury and then decreased. NR2A in the dorsal regions decreased to a plateau at 12 h after trauma. The levels of NR2B were lowest in the death group in the brainstem. Serum zinc was positively correlated with NR2A and 2B and negatively correlated with zinc in the brain. Correlations were also found between the brainstem NR2A and that of the dorsal brainstem, as well as between brainstem NR2A and changes in NR2B. There was a negative correlation between zinc and NR2A.

Conclusions: Severe trauma led to an acute reduction of zinc enhancing oxidative stress and the changes of NMDAR causing the neurotoxicity of the nerve cells. This may be a mechanism for the occurrence of MODS or death after trauma.
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http://dx.doi.org/10.12659/MSM.895075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4571529PMC
September 2015

A randomized controlled trial of acupuncture to treat functional constipation: design and protocol.

BMC Complement Altern Med 2014 Oct 29;14:423. Epub 2014 Oct 29.

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, People's Republic of China.

Background: Functional constipation (FC) is a common functional gastrointestinal disorders (FGIDs) which has a major impact on the quality of life. Acupuncture is widely used as an alternative and complementary medicine (CAM) for FC, but the available evidence of its effectiveness is scarce. Therefore, we will perform a randomized controlled trial to determine whether acupuncture improves symptom and quality of life in FC patients more effectively than sham acupuncture or gastrointestinal prokinetic agent. This article will report the protocol of the trial.

Methods: The current trial is a multicenter, randomized, three-arm controlled study undergoing in China. About 243 people who aged from 18 to 65 years with FC will be recruited in this study. These participants will be randomly allocated into three treatment groups, including electro-acupuncture (EA), Mosapride (M) and Mosapride & Sham Electro-acupuncture (MS) groups in a 1:1:1 ratio. Both the EA and sham EA receives 16 sessions of needling at Quchi (LI11) and Shangjuxu (ST37) during 4 weeks of treatment, and a follow-up period of 4 weeks. These groups will be compared on the primary outcomes of the number of times of defecation at baseline and 2, 4, 8 weeks after randomization. The secondary outcome measures include: stool consistency, intensity of defecating difficulty, MOS item Short Form health survey (SF-36), Self-Rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), and the validated Patient Assessment of Constipation Quality of Life (PAC-QOL). These outcomes are measured at baseline and 2, 4 weeks after randomization, but SF-36 is measured at baseline and 4 weeks after randomization.

Discussion: This study will supply significant evidence for using acupuncture to treat FC, and will help us to observe whether it is a therapeutic effect rather than a placebo effect.
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http://dx.doi.org/10.1186/1472-6882-14-423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287335PMC
October 2014

Jiao tai wan attenuates hepatic lipid accumulation in type 2 diabetes mellitus.

Evid Based Complement Alternat Med 2013 10;2013:567045. Epub 2013 Nov 10.

Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Jiao Tai Wan (JTW), a Chinese herbal formula containing Rhizoma Coptidis and Cortex Cinnamomi, has been used for diabetic treatment for many years. The aim of this study was to determine the main components in JTW and to investigate the effects of JTW on hepatic lipid accumulation in diabetic rats and humans. JTW extract was prepared and the main components were assayed by HPLC. An animal model of diabetes mellitus was established and JTW was administered intragastrically. In the clinical study, diabetic patients with poor glycemic control were treated with JTW. Blood glucose and lipid parameters, liver histology, hepatic triglyceride content and lipogenic gene expression were examined. Our data demonstrated that JTW significantly improved hyperglycemia, hyperlipidemia and hepatic lipid accumulation in diabetic rats. This was accompanied by the down-regulation of acetyl coenzyme A carboxylase (ACC) and fatty acid synthase (FAS) protein expressions, and the up-regulation of AMP-activated protein kinase (AMPK) and phosphorylated-ACC (pACC) protein expressions in the liver tissues. Diabetic patients also exhibited decreases in their hepatic triglyceride content. The results suggest that JTW attenuates hepatic lipid accumulation in diabetic rats and humans. These beneficial effects are possibly associated with the inhibition of lipogenic gene expression in the liver.
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http://dx.doi.org/10.1155/2013/567045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844214PMC
December 2013

Prognostic value of blood zinc, iron, and copper levels in critically ill children with pediatric risk of mortality score III.

Biol Trace Elem Res 2013 Jun 8;152(3):300-4. Epub 2013 Feb 8.

Department of Forensic Medicine, Shantou University Medical College, Shantou, 515041, Guangdong Province, People's Republic of China.

We aimed to explore the association of blood Zn, Fe, and Cu concentrations and changes in the pediatric risk of mortality (PRISM) score in critically ill children, to predict prognosis. We included 31 children (22 boys and 9 girls, 1 month to 5 years old), who had been admitted to the intensive care unit of our hospital and who were critically ill according to PRISM score of III. Another 20 children (12 boys, 8 girls, 3 months to 5 years old) who were brought to the hospital for a health checkup were included as controls. We recorded clinical data, time in the intensive care unit, prognosis, and PRISM III score for critically ill children. Blood Cu, Zn, and Fe values were measured by inductively coupled plasma atomic emission spectrophotometry. Zn and Fe levels were significantly lower in patients than in controls (all p < 0.05). Cu levels differed between patients and controls, but not significantly (p > 0.05). In ill children, blood Zn and Fe concentrations were inversely correlated with PRISM III score (Zn: r = -0.36; Fe: r = -0.50, both p < 0.05), with no significant correlation of blood Cu level and PRISM III score (r = -0.13, p > 0.05). Serious illness in children may lead to decreased Zn and Fe blood concentrations. Zn and Fe supplements may be beneficial for critically ill children.
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http://dx.doi.org/10.1007/s12011-013-9623-xDOI Listing
June 2013

Altered levels of trace elements in acute lung injury after severe trauma.

Biol Trace Elem Res 2012 Jun 3;147(1-3):28-35. Epub 2011 Dec 3.

Department of Forensic Medicine, Shantou University Medical College, Shantou, Guangdong, People's Republic of China.

Trace element (TE) supplementation can reduce the incidence of multiple organ failure after severe trauma. The lung plays a main role in post-injury multiple organ failure. In the present study, the relationship between TEs and acute lung injury (ALI) post-injury was investigated in a rabbit model of severe trauma with an injury severity score of 27. New Zealand white rabbits were randomly assigned to trauma-control, trauma-TE groups, and a control group. During days 1-5 post-trauma, each rabbit in the trauma-TE group received 0.1 ml multi-TE compound intraperitoneally to give a daily dose of 32.50 mg/kg of Zn, 6.35 mg/kg of Cu, 1.38 mg/kg of Mn, and 0.16 mg/kg of Se. Concentrations of blood and lung selenium (Se), copper (Cu), zinc (Zn), and manganese (Mn) were measured at 6 and 24 h, as well as 3, 6, 9, and 14 days after trauma. Levels of glutathione peroxidase (GPx), total superoxide dismutase (SOD), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and malondialdehyde (MDA) in serum and lung tissue and the level of intercellular adhesion molecular-1 (ICAM-1) in serum were detected simultaneously. In addition, the lung coefficient (LC) and the lung permeation index (LPI) were measured. Serum and lung Zn, Se, and Mn levels decreased dramatically by 6 h after trauma in both experimental groups. Cu showed no significant changes after trauma. The serum and lung GPx and SOD levels in the experimental group decreased significantly on days 1 and 3, respectively. Serum and lung MDA began to increase on day 3 in the trauma group but increased less after TE supplementation. Serum ICAM-1 peaked on day 6 in the experimental group. LC and LPI increased gradually post-trauma, peaking on days 6 and 9, respectively. In conclusion, an acute lung injury causes declines of the levels of TEs in serum and lung which can be significantly prevented by TE supplementation and which can also mitigate some of the morphological and biomechanical changes in ALI.
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http://dx.doi.org/10.1007/s12011-011-9281-9DOI Listing
June 2012

Alcoholism and traumatic subarachnoid hemorrhage: an experimental study on vascular morphology and biomechanics.

J Trauma 2011 Jan;70(1):E6-12

Department of Forensic Medicine, Shantou University Medical College, Shantou City, Guangdong Prov., China.

Background: Traumatic subarachnoid hemorrhage (TSAH) related to alcohol abuse is a notable risk factor. Here, we investigated the vascular morphology and biomechanics of TSAH in rat models of acute alcoholic intoxication and chronic alcoholism rats to explore the possible mechanisms of TSAH.

Methods: Sixty male Sprague-Dawley rats were divided into acute alcoholic intoxication and chronic alcoholism groups. Edible spirituous liquor (56% vol/vol) was intragastrically given (15 mL/kg) once to the rats in the acute group, and given twice daily (8 mL/kg for 2 weeks and 12 mL/kg for another 2 weeks) to rats in the chronic group. A self-made instrument was used to inflict head injury. Whole brain, arterial blood, and thoracic aorta of rats were sampled for morphologic and biomechanical examination.

Results: Compared with the acute alcoholic rats, the chronic alcoholic rats showed significant morphologic and biomechanical changes: (1) decreased body weight (p<0.05), (2) higher morbidity and mortality from TSAH (p<0.01), (3) greater mean thickness of vascular wall of subarachnoid small arteries and each layer thickness of thoracic aorta (p<0.05), (4) decreased failure load and corresponding extensibility (60 kPa and limit load) of thoracic aorta, and (5) increased elastic modulus (30 kPa, range in physiologic stress) (p<0.05).

Conclusions: Chronic alcoholism can induce the morphologic and biomechanical changes in cerebral vessels and thoracic aorta. The synergistic effect of alcohol abuse and minor blow may be one of the mechanisms of TSAH. High blood pressure from long-term alcohol abuse is also a notable factor.
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http://dx.doi.org/10.1097/TA.0b013e3181cda3b9DOI Listing
January 2011

Predatory stress induces hippocampal cell death by apoptosis in rats.

Neurosci Lett 2007 Jun 2;421(2):115-20. Epub 2007 Jun 2.

Department of Forensic Medicine, Shantou University Medical College, Shantou 515031, Guangdong, PR China.

It is commonly accepted that stress may induce brain damage, especially in hippocampal formation, but the mechanisms that cause such damage are not well understood. This paper investigated the impacts of predatory stress on the hippocampus using cat-exposure to rats. The rats were divided randomly into single stress, 2-, 4-week stress and control group. ISEL and electronic microscope were employed to confirm the occurrence of apoptosis in hippocampus. Our results showed that repeated predatory stresses may result in a pattern of intensive behavioral and endocrinal responses, and lead to the neuronal cell loss in hippocampus. ISEL results displayed that there are some ISEL-positive cells with characteristic apoptotic ultrastructural changes found most in hippocampal CA3 and CA1 in the repeated stressed rats. These results indicate that apoptosis may be one of the most important neuropathological mechanisms for cell loss or hippocampal atrophy induced by predatory stress. Meanwhile, significant positive correlation between serum cortisol level and the number of apoptotic cells in CA3 supports that excessive GCs due to predatory stress, is associated with hippocampal cell apoptosis.
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http://dx.doi.org/10.1016/j.neulet.2007.04.084DOI Listing
June 2007

Expression and bioactivity of recombinant segments of human perforin.

Biochem Cell Biol 2007 Apr;85(2):203-8

Department of Forensic Medicine, Shantou University Medical College, 22 Xinling Road, Shantou 0754, Guangdong province, China.

The aim of the study was to prepare an active recombinant human perforin by comparing 5 candidate segments of human perforin. Full-length perforin, MAC1 (28-349 aa), MAC2 (166-369 aa), C-100, and N-60 of human perforin were selected as candidate active segments and designated, respectively, HP1, HP2, HP3, HP4, and HP5. The target genes were amplified by PCR and the products were individually subcloned into pGEM-T. The genes for HP1, HP2, HP3, and HP5 were subcloned into pET-DsbA, whereas pET-41a (+) was used as the expression vector of HP4. The fusion proteins were expressed in Escherichia coli BL21pLysS(DE3) and purified using nickel nitrilotriacetic acid (NTA) agarose affinity chromatography. The hemolysis microassay was used as an activity assay of fusion protein. From this study, we obtained the recombinant plasmids pGEM-T-HP1, -HP2, -HP3, -HP4 and -HP5, consisting of 1600, 960, 600, 300bp, and 180, respectively. From these recombinant plasmids, expression plasmids were successfully constructed and expressed in E. coli BL21pLysS(DE3). The resultant fusion proteins, affinity purified using Ni-NTA, were approximately 80, 58, 45, 44, and 30 kDa, respectively. The recombinant proteins were assayed for activity on hemolysis. HP2 and HP5 were the only recombinant proteins that were active in hemolysis, and the hemolytic function was concentration dependent. These results demonstrate that active recombinant forms of perforin can be synthesized in a prokaryote model. The recombinant N-60 and MAC1 (28-349 aa) of human perforin have the function of forming pores. Our study provides the experimental basis for further investigation on the application of perforin.
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http://dx.doi.org/10.1139/O07-017DOI Listing
April 2007

Cloning and characterization of a LASS1-GDF1 transcript in rat cerebral cortex: conservation of a bicistronic structure.

DNA Seq 2007 Apr;18(2):92-103

Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong Province, 515041, People's Republic of China.

LASS1 is the mammalian homologue of yeast longevity-assurance gene 1 (LAG1) which is differentially expressed during the yeast replicative life span and was shown to play a role in determining yeast longevity. Growth/differentiation factor 1 (GDF1) is a transforming growth factor-beta family member that was originally isolated from an mouse embryo cDNA library. GDF1 is expressed specifically in the nervous system and functions in left-right patterning of the mouse embryo. To explore the potential role of LASS1 in rat neuron aging, northern blot analysis for LASS1 mRNA was performed and detected a 2.7 kb transcript in adult rat cerebral cortex. Cloning and sequence analysis revealed that this transcript contains two nonoverlapping open reading frames (ORFs), LASS1 and GDF1, which are quite different to the predicted sequences in GenBank (accession number XM_224734 and XM_224733). The alignment with the rat genome database revealed this transcript matches the sequence of rat chromosome 16 genomic contig (GenBank accession number NW_047470) between nucleotide positions 1935060th and 1919439th, which contains eight exons and seven introns. Multiple sequence analysis revealed high conservation of the two ORFs. The phylogenetic analysis showed very close evolutionary relationship among rat, mouse and human. It raises the possibility that this mRNA may give rise to two different proteins and the conserved bicistronic structure might be of unknown significance.
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http://dx.doi.org/10.1080/10425170601060947DOI Listing
April 2007

Heat stroke deaths caused by electric blankets: case report and review of the literature.

Am J Forensic Med Pathol 2006 Dec;27(4):324-7

Department of Forensic Pathology, Santou University, Santou, PR China.

Heat stroke is the most serious and potentially life-threatening condition of the heat-related illnesses. Heat stroke deaths caused by electric blanket are rarely reported. In this paper, we report 2 cases of fatal heat stroke caused by overheating from electric blankets in winter. One was a 41-year-old man who was found unresponsive in bed on an electric blanket. His wife shared the same bed with him and was found unconscious. The wife's axillary temperature was 40 degrees C (104 degrees C) when she was admitted to the hospital. She fully recovered after medical treatment. The husband was pronounced dead at scene, with rectal temperature at 41.2 degrees C (106.2 degrees C). The other was a 13-year-old girl who was found dead in bed on an electric blanket, with rectal temperature at 41 degrees C (105.8 degrees F). The literature is reviewed, and the pertinent findings, including scene investigations, postmortem examination, the risk and mechanism of fatal heat stroke caused by using electric blanket, are discussed.
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http://dx.doi.org/10.1097/01.paf.0000233567.51784.31DOI Listing
December 2006

[Comparisons of hemodynamics in 1-way and 2-way CABG].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2006 Apr;23(2):295-9

Medical School of Shantou University, Shantou 515031, China.

For the purpose of improving the hemodynamics of CABG, the authors presented a new amelioration measurement of symmetric 2-way bypass graft. The physiological blood flow in 1-way and 2-way bypass graft of coronary is simulated with finite element method, and the data on computational hemodynamics of these two cases were compared. The temporal-spatial distributions of hemodynamics during the cardiac cycle such as flow patterns and wall shear stress in the vicinity of anastomosis were analyzed. The study results showed that 2-way bypass graft was of more rational hemodynamics when compared with 1-way bypass graft, and it could improve the flow conditions and decrease the probability of restenosis.
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April 2006

Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion.

Indian J Med Res 2006 Feb;123(2):131-8

Forensic Pathology Department of Medical School of Shantou University, Shantou, Guangdong Province, PR China.

Background & Objective: Myocardial hypofunction could lead to the derangement of brain functions. The expression of c-fos and heat shock protein (hsp) genes was recognized as markers of neural cell injury. We under took this study to investigate the influence of myocardial ischaemia and reperfusion (I/R) on these molecular events in the rat brain tissue and changes in mean arterial blood pressure (MAP) and the cerebral blood flow velocity (CBFV), to understand the basis of cerebral pathology following cardiac ischaemia and reperfusion.

Methods: Healthy rats (n=42) were randomly allocated into seven groups: sham-operated (A); myocardial ischaemia for 15 min, followed by 0.5, 1, 2, 4 and 6 h of reperfusion respectively (B2-B6) and hypovolemia at medium level (C). I/R rat models were established by ligating the anterior branch of the left coronary artery. Expressions of the c-fos, hsp70, hsp27 and hsp90 genes in the cerebrum, cerebellum, medullaoblongata and hippocampus, were studied with immunohistochemistry and in situ hybridization. The MAP and CBFV of the rats were also measured.

Results: The expressions of c-fos and HSP70 in brain tissue increased after myocardial ischaemia/ reperfusion, with the strongest signal appearing in the hippocampus and cerebral cortex, while labeling for HSP27 and HSP90 alpha were not detectable in any of the experimental groups. The expressions of c-fos mRNA and hsp70 mRNA shared the similar characteristics with their encoding proteins. MAP, reflection of cardiac function and cerebral blood flow decreased following cardiac ischaemia and reperfusion.

Interpretation & Conclusion: Our findings suggested that the brain damages occurred from the early phase of myocardial I/R. The exact mechanism of cerebral tissue injuries induced by myocardial I/R is not known. Further studies need to be done to throw light on these aspects.
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February 2006

[Perforin and granzyme--the perfect material of biologic missile].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2005 Oct;22(5):1075-7

Department of Forensic Medicine, Medical College of Shantou University, Shantou 515041, China.

Perforin and granzyme are important effector molecules in cytolytic cells. They can induce apoptosis of tumor cells and infection cells. The research of biologic missile is noticed with the progress in therapy targeting to the disease. It is supposed that perforin and granzyme play an important role in biological missiles because of their biologic function and structure. A review on several aspects of these effector molecules is presented.
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October 2005

[Development on study of zinc deficiency and immunity].

Wei Sheng Yan Jiu 2004 Jul;33(4):518-20

Shantou Medical College, Shantou University, Shanton 515041, China.

In this paper, the progress on study of zinc deficiency and immunity including the relationship between zinc deficiency and the apoptosis of T cell, B cell, the expression of MT, immunological function of red cell, and non-specific immune system were reviewed.
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July 2004

Effects of naloxone on the long-term potentiation of EPSPs from the pathway of Schaffer collateral to CA1 region of hippocampus in aged rats with declined memory.

Brain Res 2004 Jan;996(1):111-6

Department of Forensic Medicine, Medical College, Shantou University, 22 Rd. Xinlin, Shantou, 515031, Guangdong, PR China.

Morris water maze (MWM) was employed to distinguish the aged rats with declined memory to investigate the effect of naloxone on the synaptic plasticity of hippocampus in declined memory aged rats. After administration with naloxone for 7 days, LTP of excitatory post-synaptic potentials (EPSPs) from Schaffer collateral to CA1 region was recorded. The results showed that the maintenance of LTP of EPSPs from Schaffer collateral to CA1 subfield in isolate hippocampal brain slice was prolonged by naloxone with improved Morris water maze performance and reduced threshold of EPSPs. It is suggested that naloxone can improve learning and memory through enhancement of the synaptic plasticity of hippocampus in aged rats with declined memory.
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http://dx.doi.org/10.1016/j.brainres.2003.10.017DOI Listing
January 2004

[Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats].

Fa Yi Xue Za Zhi 2003 ;19(2):65-7

Shenzhen Criminal Science & Technology Institute, Shenzhen 518008, China.

Objective: To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.

Methods: The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.

Results: After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.

Conclusion: c-fos mRNA detection may become an important method for diagnosis of I/R.
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May 2004

Effects of selenium and iodine on c-fos and c-jun mRNA and their protein expressions in cultured rat hippocampus cells.

Biol Trace Elem Res 2002 ;90(1-3):175-86

Department of Pathology, Shantou University Medical College, Guangdong, 515031 China.

The objective of the study was to investigate the effect of selenium (Se) and iodine (I) for expressions of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus cells in selenium- and iodine-containing medium. With cultured rat hippocampus cells, the different doses of Se and I were added into the medium. The expressions of c-fos/c-jun in cultured rat hippocampus cells (1 d, 3 d, 5 d, 7 d, and 10 d) were studied by using both in situ hybridization histochemistry and SABC immunohistochemistry techniques. Both Se and I could enhance expressions of c-fos and c-jun mRNA and their proteins, especially c-jun mRNA expression in the Se and I united group. Se and I could promote the expressions of c-fos and c-jun and thus may affect the differentiation and development of hippocampus neurons.
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http://dx.doi.org/10.1385/BTER:90:1-3:175DOI Listing
July 2003

Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.

Zhonghua Bing Li Xue Za Zhi 2002 Jun;31(3):245-9

Department of Pathology, Shantou University Medical College, Shantou 515031, China (Email:

Objective: To study the effect of selenium (Se) and iodine (I) and the compound of both on the proto-oncogenes c-fos and c-jun mRNA and their protein expression in the cultured rat hippocampus neurons.

Methods: Using the technique of serum free hippocampus neuron culture, different doses of Se and I and Se + I compound were added into the medium. The expression of the mRNA of c-fos, c-jun in hippocampus neurons cultured for 1, 3, 5, 7 and 10 d were studied using both in situ hybridization and SABC immunohistochemical technique.

Results: Both Se and I could enhance the expression of c-fos, c-jun mRNA and their proteins, especially the combination of I and Se able to give a remarkable effect on c-jun mRNA expression.

Conclusions: Se and I may effect the expression of both c-fos and c-jun mRNA, especially the c-jun mRNA and its protein of hippocampus neurons, and thus may effect the differentiation and development of neurons.
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June 2002

Studies on survival and outgrowth of processes of cultured rat hippocampus neurons in containing selenium and free serum medium.

Zhonghua Yu Fang Yi Xue Za Zhi 2002 May;36(3):167-71

Department of Pathology, Shantou University Medical College, Guangdong 515031, China.

Objective: To study the effect of selenium on cultured newborn rat's hippocampus neurons survival and outgrowth development.

Methods: Using the technique of primary culture of hippocampal neurons of newborn rat. The different dose of Se (62.5 microgram/L, 125.0 microgram/L, 182.5 microgram/L) were added into the medium at same time. We not only investigated the number of survival of neurons on 1 - 14 d and 1d to 10 d in with and without serum containing-Se medium, but also observed the length outgrowth of the neurite at 16 h, 24 h, 36 h, 48 h during culture.

Results: Selenium could obviously enhance the outgrowth of early processed in 10% fetal serum medium and average length of neurite outgrowth is 15 - 20 micrometer more longer than control groups (P < 0.01) and selenium could also increase the livability of neurons and prolong survival time of cultured neurons in serum-free medium.

Conclusions: Selenium may play a very important role for early processed growth and development of hippocampal neurons.
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May 2002
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