Publications by authors named "Xiaofeng Yin"

68 Publications

Identification of potential microRNAs and KEGG pathways in denervation muscle atrophy based on meta-analysis.

Sci Rep 2021 Jun 30;11(1):13560. Epub 2021 Jun 30.

Department of Orthopedics and Traumatology, Peking University People's Hospital, Beijing, 100044, China.

The molecular mechanism of muscle atrophy has been studied a lot, but there is no comprehensive analysis focusing on the denervated muscle atrophy. The gene network that controls the development of denervated muscle atrophy needs further elucidation. We examined differentially expressed genes (DEGs) from five denervated muscle atrophy microarray datasets and predicted microRNAs that target these DEGs. We also included the differentially expressed microRNAs datasets of denervated muscle atrophy in previous studies as background information to identify potential key microRNAs. Finally, we compared denervated muscle atrophy with disuse muscle atrophy caused by other reasons, and obtained the Den-genes which only differentially expressed in denervated muscle atrophy. In this meta-analysis, we obtained 429 up-regulated genes, 525 down-regulated genes and a batch of key microRNAs in denervated muscle atrophy. We found eight important microRNA-mRNA interactions (miR-1/Jun, miR-1/Vegfa, miR-497/Vegfa, miR-23a/Vegfa, miR-206/Vegfa, miR-497/Suclg1, miR-27a/Suclg1, miR-27a/Mapk14). The top five KEGG pathways enriched by Den-genes are Insulin signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway and B cell receptor signaling pathway. Our research has delineated the RNA regulatory network of denervated muscle atrophy, and uncovered the specific genes and terms in denervated muscle atrophy.
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http://dx.doi.org/10.1038/s41598-021-92489-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245453PMC
June 2021

Beyond Fibonacci patterns and the golden angle: phyllotactic variations and their cellular origin.

J Plant Res 2021 05;134(3):369-371

Center for Education in Liberal Arts and Sciences, Osaka University, 1-16 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan.

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http://dx.doi.org/10.1007/s10265-021-01310-7DOI Listing
May 2021

A Pulse-chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Leaf Primordia.

Bio Protoc 2021 Jan 5;11(1):e3882. Epub 2021 Jan 5.

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.

In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are difficult. The few existing methods for detection and quantification of cell division orientation are either inefficient or laborious. Here, we describe a pulse-chase strategy using a 5-ethynyl-2'-deoxyuridine (EdU) labeling assay. Plant tissues are first incubated with EdU for a short period (pulse), followed by a long incubation without EdU (chase). Using this method, the positions of daughter cells are easily detected and can be used to quantify cell division orientation. Our protocol is rapid and very efficient for quantitative analysis of cell division orientation, and can be applied to both model and non-model plant species. .
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http://dx.doi.org/10.21769/BioProtoc.3882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952930PMC
January 2021

Fermented Soybean Meal Affects the Reproductive Performance and Oxidative Status of Sows, and the Growth of Piglets.

Animals (Basel) 2021 Feb 24;11(3). Epub 2021 Feb 24.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai 200240, China.

This study aimed to investigate the effect of the fermented soybean meal on the reproductive performance, oxidative stress and colostrum composition of sows, and the growth performance of their progeny. A total of 44 sows were allotted to four dietary groups ( = 11/group). The dietary groups included the basal diet group (control) and the treatment groups in which soybean meal in the basal diet was replaced with 2%, 4%, and 6% fermented soybean meal, respectively. The experimental diets were fed to the sows from the 78th day of gestation to the 21st day of lactation. Replacing soybean meal in the basal maternal diet with the fermented soybean meal decreased the levels of malondialdehyde, cortisol, and 8-iso-prostaglandinF2α in the serum of sows and increased the average weight of piglets on the 14th day and the 21st day after birth. The activity of superoxide dismutase in the serum of sows was increased in the group with 4% fermented soybean meal on the 17th day of lactation. The levels of estrogen and growth factors in the serum of sows were enhanced in the group with 6% fermented soybean meal. In the colostrum, the levels of the protein and the immunoglobulin G were enhanced in the group with 4% fermented soybean meal. In conclusion, replacing the soybean meal in the basal maternal diet with the fermented soybean meal attenuates the oxidative stress status of the gestational and lactational sows, and enhances the average weight of their offspring.
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http://dx.doi.org/10.3390/ani11030597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996358PMC
February 2021

Phyllotaxis: from classical knowledge to molecular genetics.

Authors:
Xiaofeng Yin

J Plant Res 2021 May 7;134(3):373-401. Epub 2021 Feb 7.

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.

Plant organs are repetitively generated at the shoot apical meristem (SAM) in recognizable patterns. This phenomenon, known as phyllotaxis, has long fascinated scientists from different disciplines. While we have an enriched body of knowledge on phyllotactic patterns, parameters, and transitions, only in the past 20 years, however, have we started to identify genes and elucidate genetic pathways that involved in phyllotaxis. In this review, I first summarize the classical knowledge of phyllotaxis from a morphological perspective. I then discuss recent advances in the regulation of phyllotaxis, from a molecular genetics perspective. I show that the morphological beauty of phyllotaxis we appreciate is the manifestation of many regulators, in addition to the critical role of auxin as a patterning signal, exerting their respective effects in a coordinated fashion either directly or indirectly in the SAM.
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http://dx.doi.org/10.1007/s10265-020-01247-3DOI Listing
May 2021

Analytic Expression of Quantum Discords in Werner States under LQCC.

Entropy (Basel) 2020 Jan 26;22(2). Epub 2020 Jan 26.

School of Physics & Material Science, Anhui University, Hefei 230039, China.

In this paper, quantum discords in a special kind of states, i.e., Werner states by local quantum operations and classical communication (LQCC) protocols (WLQCC states), are studied. Nineteen parameters to quantify the quantum discords are reduced to four parameters in terms of properties of Werner states and quantum discord. In the case of orthogonal projective measures, analytic expression of quantum discords in WLQCC states is analytically worked out. Some properties of the quantum discord in the WLQCC states are obtained, especially the variation relations between the quantum discords and the parameters characterizing the WLQCC states. By virtue of numerical computations, quantum discords in a Werner state before and after LQCC protocols are compared. It is found that quantum discord in any WLQCC state cannot exceed that in the original Werner state.
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http://dx.doi.org/10.3390/e22020147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7516559PMC
January 2020

High Oxygen Evolution Activity of Tungsten Bronze Oxides Boosted by Anchoring of Co at Nb Sites Accompanied by Substantial Oxygen Vacancy.

Adv Sci (Weinh) 2020 Nov 29;7(22):2002242. Epub 2020 Sep 29.

Institute for Superconducting & Electronic Materials (ISEM) Australia Institute for Innovative Materials Innovation Campus University of Wollongong Squires Way North Wollongong NSW 2500 Australia.

The participation of lattice oxygen in the oxygen evolution reaction (OER) process has been proved to be faster in kinetics than the mechanisms where only metal is involved, although activating the lattice oxygen in the traditional rigid structures remains a big challenge. In this work, efforts are devoted to exploring a new flexible structure that is competent in providing large amounts of oxygen vacancies as well as offering the freedom to manipulate the electronic structure of metal cations. This is demonstrated by anchoring low valence state Co at high valence state Nb sites in the tetragonal tungsten bronze (TTB)-structured SrBaNb Co O , with different ratios of Co to Nb to optimize the Co substitution proportion. It is found that the occupation of Co in the Nb sites gives rise to the generation of massive surface oxygen vacancies (O), while Co itself is stabilized in Co by adjacent O. The coexistence of O and LS Co enables an oxygen intercalation mechanism in the optimal SBNC45 with specific activity at 1.7 V versus reversible hydrogen electrode that is 20 times higher than for the commercial IrO. This work illuminates an entirely new avenue to rationally design OER electrocatalysts with ultrafast kinetics.
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http://dx.doi.org/10.1002/advs.202002242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675188PMC
November 2020

Neutrophil peptide-1 promotes the repair of sciatic nerve injury through the expression of proteins related to nerve regeneration.

Nutr Neurosci 2020 Oct 15:1-11. Epub 2020 Oct 15.

Department of Trauma and Orthopedics, Peking University People's Hospital, Peking University, Beijing, People's Republic of China.

Small-molecule polypeptide neutrophil peptide 1 (NP-1) was reported to promote the regeneration of the sciatic nerve after denervation, but the mechanisms underlying this effect of NP-1 are unclear. Here, we established a Sprague-Dawley rat model of crush injury to study the effect of a single intermuscular injection of NP-1 on the repair of injured peripheral nerves and elucidate the possible underlying mechanism. 39 rats were randomly selected to join this study and divided into the blank control group (normal group, n=9), experimental group (NP-1 group, n=15), and negative control group (NS group, n=15). The dynamic expression of cytokines in different groups of nerve tissues during Wallerian degeneration was observed using protein chips at different time points after injury. Recovery of injured nerves was determined based on the general condition, local gross morphology of the nerve suture site, sciatic nerve function index, neuroelectrophysiology, and osmic acid staining at 6 weeks after the surgery. The recovery of effector function was determined based on wet weight, hematoxylin-eosin staining, modified Gomori staining, and nicotinamide adenine dinucleotide-tetrazolium reductase staining at 6 weeks after the surgery. It was found that a single topical administration of NP-1 promoted sciatic nerve regeneration after crush injury and affected the expression of proteins related to neurotrophy, inflammation, cell chemotaxis, and cell generation pathways.
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http://dx.doi.org/10.1080/1028415X.2020.1792617DOI Listing
October 2020

Effects of NP-1 on proliferation, migration, and apoptosis of Schwann cell line RSC96 through the NF-κB signaling pathway.

Am J Transl Res 2020 15;12(8):4127-4140. Epub 2020 Aug 15.

Department of Trauma and Orthopedics, Peking University People's Hospital, Peking University Beijing, China.

Peripheral nerve injury is a common refractory disease in the clinic that often leads to dysfunction of movement and sensation. Different from other tissue injuries, peripheral nerve injury needs a longer time for regeneration. Therefore, effective drug therapy is needed to promote nerve regeneration in the treatment of peripheral nerve injury. Our preliminary studies have shown that continuous intramuscular injection of NP-1 promotes the regeneration of injured sciatic nerve in rats, but the mechanisms were still unknown. Schwann cells are very important cells in the formation of myelin sheath of peripheral nerves and participate in the repair and regeneration of peripheral nerve injury. To further investigate the effect of NP-1 on rat Schwann cells and the underlying mechanism, different concentrations of NP-1 were used to treat rat Schwann cell line RSC96. Light microscopy, CCK-8 assay, cell scratch assay, and special cell staining were performed to investigate RSC96 cell aging and apoptosis. mRNA and protein expression of NF-κB signaling pathway-related factors were determined using qPCR and immunohistochemistry respectively. Light microscopy, CCK-8 assay, cell scratch assay, and special cell staining showed NP-1 could improve the ability of proliferation, immigration of Schwann cells. QPCR and immunohistochemistry showed NP-1 influenced the expression of multiple factors associated with nerve regeneration which NF-κB signaling pathway played a key role. The results show that NP-1 promoted the proliferation and migration of RSC96 cells and inhibited cell aging and apoptosis possibly through the NF-κB signaling pathway. These findings provide a potential target for clinical treatment of peripheral neuropathy and experimental data support.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476162PMC
August 2020

Acute and Subacute Toxicity Study of Graphene-Based Tumor Cell Nucleus-Targeting Fluorescent Nanoprobes.

Mol Pharm 2020 07 10;17(7):2682-2690. Epub 2020 Jun 10.

Tumor Precision Targeting Research Center, School of Environmental and Chemical Engineering, Shanghai University, 99 Shangda Street, Shanghai 200444, P. R. China.

Graphene-based tumor cell nuclear targeting fluorescent nanoprobes (GTTNs) were synthesized in our laboratory as a kind of nanomaterial and showed good performance for both and imaging. GTTNs directly cross the cell membrane and specifically target the tumor cell nucleus a cell membrane permeability targeting (CMPT) mechanism, which takes advantage of the increased permeability of the tumor cell membranes. GTTNs with a CMPT mechanism achieve high targeting efficiency in tumor tissues. With the tumor cell nucleus-targeting characterization, the GTTN distinguishes tumor cells at the single-cell level and recognizes the tumor tissue interface in a very early stage and shows great potential in clinical applications. Toxicity studies are extremely critical for clinical applications. Therefore, we studied the acute and subacute toxicity of GTTNs using an method and examined the following experimental indicators: mouse body weight, organ coefficients, serum biochemical parameters, and histological changes. The results showed that there were no significant differences in any indicators between the experimental and control mice. We also used an method to study the cytotoxicity of GTTNs in GES-1 (gastric epithelial cell) cells. Surprisingly, the results demonstrated over 80% cell viability when the incubation time reached up to 72 h under a 200 mg/L concentration of GTTNs, which indicated that GTTNs had low cytotoxicity. GTTNs barely showed any acute or subacute toxicity or cytotoxicity and , respectively, which supports their use for clinical applications.
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http://dx.doi.org/10.1021/acs.molpharmaceut.0c00380DOI Listing
July 2020

Metabolomic profiling reveals serum L-pyroglutamic acid as a potential diagnostic biomarker for systemic lupus erythematosus.

Rheumatology (Oxford) 2021 02;60(2):598-606

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangdong, Guangdong, P.R. China.

Objective: The spectrum of clinical manifestations and serological phenomena of SLE is heterogeneous among patients and even changes over time unpredictably in individual patients. For this reason, clinical diagnosis especially in complicated or atypical cases is often difficult or delayed leading to poor prognosis. Despite the medical progress nowadays in the understanding of SLE pathogenesis, disease-specific biomarkers for SLE remain an outstanding challenge. Therefore, we undertook this study to investigate potential biomarkers for SLE diagnosis.

Methods: Serum samples from 32 patients with SLE and 25 gender-matched healthy controls (HCs) were analysed by metabolic profiling based on liquid chromatography-tandem mass spectrometry metabolomics platform. The further validation for the potential biomarker was performed in an independent set consisting of 36 SLE patients and 30 HCs.

Results: The metabolite profiles of serum samples allowed differentiation of SLE patients from HCs. The levels of arachidonic acid, sphingomyelin (SM) 24:1, monoacylglycerol (MG) 17:0, lysophosphatidyl ethanolamine (lysoPE) 18:0, lysoPE 16:0, lysophosphatidyl choline (lysoPC) 20:0, lysoPC 18:0 and adenosine were significantly decreased in SLE patients, and the MG 20:2 and L-pyroglutamic acid were significantly increased in SLE group. In addition, L-pyroglutamic acid achieved an area under the receiver-operating characteristic curve of 0.955 with high sensitivity (97.22%) and specificity (83.33%) at the cut-off of 61.54 μM in the further targeted metabolism, indicating diagnostic potential.

Conclusion: Serum metabolic profiling is differential between SLE patients and HCs and depicts increased L-pyroglutamic acid as a promising bitformatomarker for SLE.
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http://dx.doi.org/10.1093/rheumatology/keaa126DOI Listing
February 2021

Effects of miR-34c-5p on Sodium, Potassium, and Calcium Channel Currents in C2C12 Myotubes.

Cell Mol Neurobiol 2020 Oct 25;40(7):1223-1230. Epub 2020 Feb 25.

Department of Orthopedics and Traumatology, Peking University People's Hospital, Beijing, China.

The aim of this study was to investigate the effects of miR-34c-5p on the main voltage-dependent ion channels in skeletal muscle cells. This study focused on the effects of miR-34c-5p on sodium, potassium, and calcium currents in C2C12 myoblasts. The miR-34c-5p overexpression group, knockdown group, and control group were differentiated for 7 days, fused into myotubes, and used for the whole-cell patch clamp recording. Compared with the control group, the whole-cell sodium current density of the other two groups had no significant changes. In the knockdown group, the delayed rectifier potassium current density was increased (statistically significant), and the whole-cell calcium channel current density did not change. In the overexpression group, the change of rectifier potassium current density was not obvious, while the peak calcium channel current density increased (- 9.23 ± 0.95 pA/pF, n = 6 cells for the overexpression group vs. - 6.48 ± 0.64 pA/pF, n = 7 cells for the control; p < 0.05). Changes in the expression of miR-34c-5p can affect the electrophysiological characteristics of calcium and potassium voltage-gated channels in C2C12 myotubes. Overexpression of miR-34c-5p increased whole-cell L-type calcium channel current (I), while miR-34c-5p knockdown increased whole-cell delayed rectifier potassium current (I).
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http://dx.doi.org/10.1007/s10571-020-00810-9DOI Listing
October 2020

Enhanced histone H3 acetylation of the PD-L1 promoter via the COP1/c-Jun/HDAC3 axis is required for PD-L1 expression in drug-resistant cancer cells.

J Exp Clin Cancer Res 2020 Feb 5;39(1):29. Epub 2020 Feb 5.

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

Background: Drug resistance is a major obstacle to treating cancers because it desensitizes cancer cells to chemotherapy. Recently, attention has been focused on changes in the tumor immune landscape after the acquisition of drug resistance. Programmed death-ligand-1 (PD-L1) is an immune suppressor that inhibits T cell-based immunity. Evidence has shown that acquired chemoresistance is associated with increased PD-L1 expression in cancer cells. However, the underlying mechanism is still largely unknown.

Methods: PD-L1 expression in three drug-resistant A549/CDDP, MCF7/ADR and HepG2/ADR cell lines was detected by qRT-PCR, western blotting and flow cytometry, and a T cell proliferation assay was performed to test its functional significance. Then, the potential roles of JNK/c-Jun, histone H3 acetylation, histone deacetylase 3 (HDAC3) and the E3 ligase COP1 in the PD-L1 increase were explored through ChIP assays and gain- and loss-of-function gene studies. Furthermore, murine xenograft tumor models were used to verify the role of JNK/c-Jun and HDAC3 in PD-L1 expression in A549/CDDP cells in vivo. Finally, the correlations of PD-L1, c-Jun and HDAC3 expression in clinical cisplatin-sensitive and cisplatin-resistant non-small cell lung cancer (NSCLC) tissues were analyzed by immunohistochemistry and Pearson's correlation coefficient.

Results: PD-L1 expression was significantly increased in A549/CDDP, MCF7/ADR and HepG2/ADR cells and was attributed mainly to enhanced JNK/c-Jun signaling activation. Mechanistically, decreased COP1 increased c-Jun accumulation, which subsequently inhibited HDAC3 expression and thereby enhanced histone H3 acetylation of the PD-L1 promoter. Furthermore, PD-L1 expression could be inhibited by JNK/c-Jun inhibition or HDAC3 overexpression in vivo, which could largely reverse inhibited CD3 T cell proliferation in vitro. PD-L1 expression was significantly increased in the cisplatin-resistant clinical NSCLC samples and positively correlated with c-Jun expression but negatively correlated with HDAC3 expression.

Conclusions: Enhanced histone H3 acetylation of the PD-L1 promoter via the COP1/c-Jun/HDAC3 axis was crucial for the PD-L1 increase in drug-resistant cancer cells. Our study reveals a novel regulatory network for the PD-L1 increase in drug-resistant cancer cells and that combined PD-L1-targeting strategies could improve T cell-based immunity in drug-resistant cancers.
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http://dx.doi.org/10.1186/s13046-020-1536-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003365PMC
February 2020

Identification of biological pathways and genes associated with neurogenic heterotopic ossification by text mining.

PeerJ 2020 3;8:e8276. Epub 2020 Jan 3.

Department of Trauma and Orthopaedic Surgery, Peking University People's Hospital, Beijing, China.

Background: Neurogenic heterotopic ossification is a disorder of aberrant bone formation affecting one in five patients sustaining a spinal cord injury or traumatic brain injury (SCI-TBI-HO). However, the underlying mechanisms of SCI-TBI-HO have proven difficult to elucidate. The aim of the present study is to identify the most promising candidate genes and biological pathways for SCI-TBI-HO.

Methods: In this study, we used text mining to generate potential explanations for SCI-TBI-HO. Moreover, we employed several additional datasets, including gene expression profile data, drug data and tissue-specific gene expression data, to explore promising genes that associated with SCI-TBI-HO.

Results: We identified four SCI-TBI-HO-associated genes, including GDF15, LDLR, CCL2, and CLU. Finally, using enrichment analysis, we identified several pathways, including integrin signaling, insulin pathway, internalization of ErbB1, urokinase-type plasminogen activator and uPAR-mediated signaling, PDGFR-beta signaling pathway, EGF receptor (ErbB1) signaling pathway, and class I PI3K signaling events, which may be associated with SCI-TBI-HO.

Conclusions: These results enhance our understanding of the molecular mechanisms of SCI-TBI-HO and offer new leads for researchers and innovative therapeutic strategies.
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http://dx.doi.org/10.7717/peerj.8276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944123PMC
January 2020

Multiple retrograde tracing methods compatible with 3DISCO clearing.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):4240-4247

Department of Trauma and Orthopedics, Peking University People's Hospital, Beijing, China.

Exploring the spatial relationship of various neuron pools in the spinal cord is crucial and difficult due to its complexity. The single-labelling tracing and sectioning were employed in previous studies exploring the distribution of spinal motor neuron pools, which could only delineate one single motor neuron pool in one specimen and could not achieve intact-tissue observation. Here, with combination of neuroanatomy tracing techniques and the optical clearing technique, we developed a multiple retrograde tracing method compatible with 3DISCO clearing. Fluoro-Gold, Fluoro-Ruby, Cholera Toxin Subunit B, Alexa Fluor 488 and 647 Conjugate were injected intramuscularly in hindlimbs of C57BL/6 adults. After labelling, the harvested spinal cords were optically cleared by 3DISCO method and imaged using confocal microscope. There were positive signals of all four tracers and four motor neurons pools targeting injected muscles were labelled. Three-dimension model of four motor neuron pools was successfully reconstructed based on tomography images showing the spatial relationship of different neuron pools. In conclusion, using this method, we first delineated the spatial relationship of four different motor neuron pools targeting four skeletal muscles in one spinal cord at the same time, which provide a holistic view of motor neuron pools in the spinal cord.
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http://dx.doi.org/10.1080/21691401.2019.1687493DOI Listing
December 2019

Fecal Metabolomics and Potential Biomarkers for Systemic Lupus Erythematosus.

Front Immunol 2019 3;10:976. Epub 2019 May 3.

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.

The role of metabolomics in autoimmune diseases has been a rapidly expanding area in researches over the last decade, while its pathophysiologic impact on systemic lupus erythematosus (SLE) remains poorly elucidated. In this study, we analyzed the metabolic profiling of fecal samples from SLE patients and healthy controls based on ultra-high-performance liquid chromatography equipped with mass spectrometry for exploring the potential biomarkers of SLE. The results showed that 23 differential metabolites and 5 perturbed pathways were identified between the two groups, including aminoacyl-tRNA biosynthesis, thiamine metabolism, nitrogen metabolism, tryptophan metabolism, and cyanoamino acid metabolism. In addition, logistic regression and ROC analysis were used to establish a diagnostic model for distinguishing SLE patients from healthy controls. The combined model of fecal PG 27:2 and proline achieved an area under the ROC curve of 0.846, and had a good diagnostic efficacy. In the present study, we analyzed the correlations between fecal metabolic perturbations and SLE pathogenesis. In summary, we firstly illustrate the comprehensive metabolic profiles of feces in SLE patients, suggesting that the fecal metabolites could be used as the potential non-invasive biomarkers for SLE.
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http://dx.doi.org/10.3389/fimmu.2019.00976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509220PMC
October 2020

Lower Expression of Gelsolin in Colon Cancer and Its Diagnostic Value in Colon Cancer Patients.

J Cancer 2019 30;10(5):1288-1296. Epub 2019 Jan 30.

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

Colon cancer is one of the most common malignancies causing the majority of cancer-related deaths. Gelsolin (GSN) has been found to be dysregulated in various cancers. However, the secreted GSN in colon cancer remains largely unknown. In the present study, we explored the expression profile of GSN in colon cancer tissues and the diagnostic value of serum GSN in colon cancer. In addition, the effects of secreted GSN in colon cancer cells were studied. We thus found that immunoreactive GSN levels were significantly lower in colon cancer tissues than those in non-tumor colon tissues. Functional studies demonstrated that secreted GSN could restrain cell invasion and migration in vitro. Mechanistically, dose dependent recombinant GSN down-regulated the expression of MMP2 and MMP9, which might restrain the process of cell invasion and migration. Furthermore, serum levels of GSN were significantly lower in colon cancer patients than those in healthy volunteers, and ROC curves showed serum level of GSN had a better diagnostic value for colon cancer (AUC=0.932) than the traditional tumor biomarker Carcinoembryonic Antigen (CEA) or Carbohydrate Antigen 19-9 (CA199). In conclusion, our results suggest that the secreted GSN restrains the invasion and migration of colon cancer cells. Meanwhile, the serum GSN may be a new biomarker for the diagnosis of colon cancer.
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http://dx.doi.org/10.7150/jca.28529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6400693PMC
January 2019

Spatial Distribution of Motor Endplates and its Adaptive Change in Skeletal Muscle.

Theranostics 2019 24;9(3):734-746. Epub 2019 Jan 24.

Department of Trauma and Orthopaedics, Peking University People's Hospital, Beijing 100044, China.

Motor endplates (MEPs) are the important interfaces between peripheral nerves and muscle fibers. Investigation of the spatial distribution of MEPs could help us better understand neuromuscular functional activities and improve the diagnosis and therapy of related diseases. Fluorescent α-bungarotoxin was injected to label the motor endplates in whole-mount skeletal muscles, and tissue optical clearing combined with light-sheet microscopy was used to investigate the spatial distribution of MEPs and in-muscle nerve branches in different skeletal muscles in wild-type and transgenic fluorescent mice. Electrophysiology was used to determine the relationship between the spatial distribution of MEPs and muscle function. The exact three-dimensional distribution of MEPs in whole skeletal muscles was first obtained. We found that the MEPs in the muscle were distributed in an organized pattern of lamella clusters, with no MEPs outside the lamella zone. Each MEP lamella was innervated by one independent in-muscle nerve branch and mediated an independent muscle subgroup contraction. Additionally, the MEPs changed along the lamella clusters after denervation and regained the initial pattern after reinnervation. The integrity and spatial distribution of MEPs could reflect the functional state of muscles. The signal absence of a certain MEP lamella could suggest a problem in certain part of the muscle. The MEP lamella clusters might be the basis of neuromuscular function, and the spatial distribution of MEPs could serve as a testbed for evaluating the functional status of muscle and the therapeutic targeting map related to MEPs.
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http://dx.doi.org/10.7150/thno.28729DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376466PMC
January 2020

Morphogenesis of flattened unifacial leaves in Juncus prismatocarpus (Juncaceae).

New Phytol 2019 04 22;222(2):1101-1111. Epub 2019 Jan 22.

Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.

To reveal the mode of morphogenesis of flattened unifacial leaves, we analysed the cell division direction and distribution on the leaf blade of Juncus prismatocarpus. Using the pulse-chase 5-ethynyl-2'-deoxyuridine method, we quantified and mapped the cell division direction on the leaf blade of J. prismatocarpus and compared the distribution of thickening cell divisions with the expression pattern of DROOPING LEAF (DL), a key gene involved in leaf blade thickening. Thickening cell divisions were the most abundant (> 45%) among all cell division directions on the leaf blade of J. prismatocarpus from the early plastochron 2 stage through the plastochron 3 stage. Mapping of cell divisions indicated that cell divisions in a particular direction were not restricted to a particular domain but were distributed diffusely throughout the entire cross-sectional area of the leaf blade. Gradient analysis indicated that the distribution of thickening cell divisions of the adaxial domain was denser than that of the abaxial domain. Contrary to the prolonged and diffuse distribution of thickening cell divisions, DL expression was transient and restricted in a narrow band. Our results suggest that a diffuse 'thickening meristem' plays the key role in the development of flattened unifacial leaves.
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http://dx.doi.org/10.1111/nph.15649DOI Listing
April 2019

Size, Shape, and Protein Corona Determine Cellular Uptake and Removal Mechanisms of Gold Nanoparticles.

Small 2018 10 21;14(42):e1801451. Epub 2018 Sep 21.

Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai, 200444, P. R. China.

Size, shape, and protein corona play a key role in cellular uptake and removal mechanisms of gold nanoparticles (Au NPs). The 15 nm nanoparticles (NP1), the 45 nm nanoparticles (NP2), and the rod-shaped nanoparticles (NR) enter into cells via a receptor-mediated endocytosis (RME) pathway. The star-shaped nanoparticles (NS) adopt not only clathrin-mediated, but also caveolin-mediated endocytosis pathways. However, the 80 nm nanoparitcles (NP3) mainly enter into the cells by macropinocytosis pathway due to the big size. Furthermore, the results indicate that the presence of protein corona can change the uptake mechanisms of Au NPs. The endocytosis pathway of NP1, NP2, and NS changes from RME to macropinocytosis pathway and NR changes from RME to clathrin and caveolin-independent pathway under the non-fetal bovine serun (FBS)-coated condition. Both FBS-coated and non-FBS-coated of five types of Au NPs are released out through the lysosomal exocytosis pathway. The size, shape, and protein corona have an effect on the exocytosis ratio and amount, but do not change the exocytosis mechanism. The systematic study of the endocytosis and exocytosis mechanism of Au NPs with different sizes and shapes will benefit the toxicology evaluation and nanomedicine application of Au NPs.
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http://dx.doi.org/10.1002/smll.201801451DOI Listing
October 2018

Dysregulated ICOS proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Exp Ther Med 2018 Oct 24;16(4):3728-3734. Epub 2018 Aug 24.

Department of Rheumatology and Immunology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Regulatory T cells (Tregs) serve an important role in the pathogenesis of rheumatoid arthritis (RA) by regulating autoimmunity and inflammation. Humans and mice contain inducible T-cell costimulator-positive (ICOS) Tregs, although their role in RA is unclear. A total of 33 patients with RA and 17 normal control (NC) subjects were examined. The proportion of ICOS Tregs in the peripheral blood and intracellular cytokine levels in these cells were assessed using flow cytometry. The percentage of ICOS Tregs increased in the cohort of patients with RA compared with the NCs. Such increases were much larger in patients with inactive RA compared with patients with active RA. Additionally, ICOS Tregs expressed multiple suppressive cytokines, including interleukin (IL)-10, transforming growth factor-β and IL-35, but expressed low levels of IL-17. Importantly, the expression of suppressive cytokines in ICOS Tregs from patients with active RA decreased, but IL-17 expression noticeably increased compared with patients with inactive RA. The present findings suggested that ICOS Tregs may perform inflammatory and inhibitory functions, and abnormal ICOS Tregs numbers and functions may contribute to the pathogenesis of RA.
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http://dx.doi.org/10.3892/etm.2018.6657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143914PMC
October 2018

Sonocatalysis of the magnetic recyclable layered perovskite oxides.

Ultrason Sonochem 2018 Dec 11;49:260-267. Epub 2018 Aug 11.

National Synchotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, PR China; CAS Key Laboratory of Materials for Energy Conversion, Department of Materials Science and Engineering, University of Science and Technology of China, Hefei 230026, PR China; Synergetic Innovation Center of Quantum Information and Quantum Physics & Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei 230026, PR China. Electronic address:

Sonocatalysis fascinates to utilize mechanical energy that universally exists in the environment. A big problem for the practical application of sonocatalysts is the incapability of recyclability, which is necessary for resource saving and secondary pollution control. In this work, BiFeCoTiO was firstly explored as a new sonocatalyst with magnetic recyclability. The magnetic catalysts can be easily collected with a magnetic bar after sonocatalytic reactions, and the structure and efficiency were kept after being recycled. Since the mechanism of sonocatalysis under ultrasonic vibration is still not fully understood, experiments including samples with different polarization and morphology, under different frequencies and intensities of ultrasonic radiation were conducted. The results suggested that the sonocatalytic efficiency was in proportion to polarization instead of morphology and a possible mechanism of squeezed model was proposed.
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http://dx.doi.org/10.1016/j.ultsonch.2018.08.008DOI Listing
December 2018

A Biomechanical Study of an Alternative Internal Fixation Method for Transverse Patella Fractures.

Orthopedics 2018 Sep 16;41(5):e643-e648. Epub 2018 Jul 16.

Pain and reoperation after fixation using tension band wiring and K-wires is not uncommon. A novel hook plate was designed to improve the treatment of patella fractures. The aim of this study was to compare the stability of the hook plate with that of tension band wiring and K-wires in a simulated patellar transverse fracture model (AO/OTA classification 34-C1.1). The authors tested 16 embalmed cadaver knee joints fixed with the hook plate and tension band wiring and K-wires under cyclic loading. Specimens underwent 100 cycles extending the knee joint from 90° of flexion to full extension at a velocity of 50 mm/min. The fracture gap was measured after the initial and last cycles. Data were assessed statistically using the t test, with significance set as P<.05. The fatigue test showed that the fracture gap after 100 cycles was 2.97±1.39 mm using tension band wiring and K-wires and 1.53±0.93 mm for the hook plate (P=.029). Six of 8 specimens in the tension band wiring and K-wires group met the failure criterion of fracture gap greater than 2 mm vs 1 in the hook plate group (P=.041). From a biomechanical point of view, the hook plate is a valid alternative to tension band wiring and K-wires for fixing patella transverse fractures. Compared with tension band wiring and K-wires, the hook plate may have superior ability in sustaining a reduced transverse patella fracture. [Orthopedics. 2018; 41(5):e643-e648.].
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http://dx.doi.org/10.3928/01477447-20180711-04DOI Listing
September 2018

How many nerve fibres can be separated as donor from an integral nerve trunk when reconstructing a peripheral nerve trauma with amplification method by artificial biochitin conduit?

Artif Cells Nanomed Biotechnol 2018 16;46(sup2):646-651. Epub 2018 Jul 16.

a Department of Orthopaedics and Traumatology , Peking University People's Hospital , Beijing , China.

Using portion of a nearby nerve trunk to reconstruct a severe nerve lesion by artificial biodegradable chitin conduit is the core practicable method based on peripheral nerve amplification regeneration. However, the quantitative influences on skeletal muscle function corresponding to the injury of the donated nerve fibres were not previously reported. Here, we aimed to explore the compensative capacity in tibialis anterior muscles of rats with the models of acute tibialis anterior nerve branch injuries. The tibialis anterior branch of deep peroneal nerve was transected in various levels each time. Both the decreased treads of maximal compound muscle action potential (CMAP) amplitude and complete tetanic tension of the tibialis anterior muscle in rats were similar with the increasing numbers of damaged nerve fibres, which showed two S-shaped curves. When the nerve injury level was less than approximately 10%, the skeletal muscle function remained normal through complete compensation of motor endplates. As the injury degree went from 10% to 85%, the muscle function was partially impaired due to the broken compensation of motor endplates. When the nerve injury level was over approximately 85%, the skeletal muscle function was totally lost. It suggests that within a certain level of nerve injury, the skeletal muscle function maintained basically unchanged via complete compensation of motor endplates. Such nerve fibres may be used as donor nerve to repair peripheral nerve injury.
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http://dx.doi.org/10.1080/21691401.2018.1466145DOI Listing
June 2019

Serum Levels of TRIM72 Are Lower among Patients with Colon Cancer: Identification of a Potential Diagnostic Marker.

Tohoku J Exp Med 2018 05;245(1):61-68

Laboratory Medicine Center, Nanfang Hospital, Southern Medical University.

Colon cancer is one of the most common malignancies causing the majority of cancer-related deaths worldwide. The tripartite motif family protein 72 (TRIM72), also known as mitsugumin 53, acts as an E3 ubiquitin ligase. TRIM72 is involved in insulin resistance and metabolic syndrome, which are risk factors of colon cancer. However, the correlation between TRIM72 and colon cancer remains unknown. In the present study, we explored the expression profile of TRIM72 in colon cancer tissues and the diagnostic value of serum TRIM72 in colon cancer. The receiver operating characteristic (ROC) curves were applied for evaluating the diagnostic value of serum TRIM72. We thus found that immunoreactive TRIM72 levels were significantly lower in colon cancer tissues than those in normal colon tissues. Moreover, serum TRIM72 levels were significantly lower in colon cancer patients than those in healthy volunteers. Importantly, the lower serum TRIM72 levels were associated with advanced clinical stage, lymph node, and distant metastases in colon cancer patients. The ROC curve analysis showed that serum TRIM72 has a superior diagnostic value (the area under the curve (AUC) = 0.829) than the traditional tumor biomarkers, carcinoembryonic antigen (CEA) (AUC = 0.707) and carbohydrate antigen 19-9 (CA199) (AUC = 0.750), and the combination of TRIM72 with CEA and CA199 showed the best diagnostic value for colon cancer (AUC = 0.928). In conclusion, serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer.
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http://dx.doi.org/10.1620/tjem.245.61DOI Listing
May 2018

Morphology effect on photocatalytic activity in BiFeNbO.

Nanotechnology 2018 Jun 12;29(26):265706. Epub 2018 Apr 12.

Department of Materials Science and Engineering, CAS Key Laboratory of Materials for Energy Conversion, University of Science and Technology of China, Hefei 230026, People's Republic of China.

In this work, the Aurivillius-phase ferroelectric BiFeNbO were synthesized by hydrothermal (BFNO-H) and solid state methods (BFNO-S), respectively. The BFNO-H shows a hierarchical morphology, which is stacked by intersecting single-crystal nanosheets with {001} and {110} exposed facets, while the BFNO-S shows disorganized micron-scale morphology. BFNO-H shows a much stronger photodegradation activity (10.4 times and 9.8 times) than BFNO-S in the visible-light photodegradation of rhodamine B (RhB) and salicylic acid. The higher photodegradation activity of BFNO-H was firstly ascribed to the hierarchical structure and the larger specific surface area (16.586 m g) because a large specific surface area can increase reactive sites and shorten photogenerated carrier migration distance. However, after being normalized by the specific surface area, BFNO-H still performs better than BFNO-S, implying that the specific surface area is not the only factor that determines the photocatalytic activity. Considering that the built-in electric field originating from spontaneous polarization in BiFeNbO has existed in both ab plane and c direction, it matches well with the {001} and {110} exposed facets of BFNO-H nanosheets. This appropriate matching in BFNO-H nanosheets may improve the separation and transmission of photogenerated electron-hole pairs and further enhance its photocatalytic activity. Moreover, the trapping experiments reveals that holes (h ) are the main active species and hole-derived oxidation is the main redox reaction during photodegradation of organic pollutions.
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http://dx.doi.org/10.1088/1361-6528/aabdbaDOI Listing
June 2018

The Whole Transcriptome Involved in Denervated Muscle Atrophy Following Peripheral Nerve Injury.

Front Mol Neurosci 2018 7;11:69. Epub 2018 Mar 7.

Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, China.

Peripheral nerve injury (PNI) usually leads to progressive muscle atrophy and poor functional recovery. Previous studies have demonstrated that non-coding ribonucleic acid (ncRNA) is a key regulator of muscle atrophy and beneficial for the treatment of PNI. We aimed to analyze the whole transcriptome involved in denervated muscle atrophy after PNI. Animal models of sciatic nerve injury were assessed at 0 (control group), 1, 2, 4, and 8 weeks after injury. The expression patterns in the whole transcriptome in the gastrocnemius muscle were profiled using RNA sequencing at each time point and compared to that obtained in the control group. Six-hundred and sixty-four long non-coding RNAs, 671 microRNAs, 236 circular RNAs, and 12,768 messenger RNAs (mRNAs) were differentially expressed (DE) after injury. Changes in some of the DE ncRNAs and mRNAs were validated using quantitative polymerase chain reaction. Gene Ontology and Kyoko Encyclopedia of Genes and Genomes analysis revealed the potential functions of and relationships among the DE ncRNAs and mRNAs. To our knowledge, this is the first study to expound the whole transcriptome involved in denervated muscle atrophy, and provides a theoretical basis for further research targeting ncRNAs.
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http://dx.doi.org/10.3389/fnmol.2018.00069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845901PMC
March 2018

Peripheral nerve intersectional repair by bi-directional induction and systematic remodelling: biodegradable conduit tubulization from basic research to clinical application.

Artif Cells Nanomed Biotechnol 2017 Dec 8;45(8):1464-1466. Epub 2017 Sep 8.

a Trauma & Orthopaedics Department , Peking University People's Hospital , Beijing , China.

In terms of the clinical effect of peripheral nerve injury repair, the biological degradable conduit 2 mm small gap tubulization is far better than the traditional epineurial or perineurium neurorrhaphy. The assumption of the bi-directional induction between the central system and the terminal effector during peripheral nerve regeneration is purposed and proved in clinical by our group. The surgical approach of transferring a portion of or the whole contralateral C7 nerve to repair a part of or the whole ipsilateral brachial plexus injury is clinically promoted, in which the most important idea and practice is to use the cone conduit designed by the group to repair thick nerves with fine nerves. Some of the patients suffering from cerebral palsy or cerebral haemorrhage and those who got cerebral infarction yet have not reached recovery after 3-6 months could regain some functions of the ipsilateral upper limb and improve the life quality by transfer of a portion of or the whole contralateral C7 nerve and connection by cone conduit.
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http://dx.doi.org/10.1080/21691401.2017.1373658DOI Listing
December 2017

Analysis of temporal expression profiles after sciatic nerve injury by bioinformatic method.

Sci Rep 2017 08 29;7(1):9818. Epub 2017 Aug 29.

Peking University People's Hospital, Beijing, China.

After Peripheral nerve injuries (PNI), many complicated pathophysiologic processes will happen. A global view of functional changes following PNI is essential for the looking for the adequate therapeutic approaches. In this study, we performed an in-depth analysis on the temporal expression profiles after sciatic nerve injury by bioinformatic methods, including (1) cluster analysis of the samples; (2) identification of gene co-expression modules(CEMs) correlated with the time points; (3) analysis of differentially expressed genes at each time point (DEGs-ET); (4) analysis of differentially expressed genes varying over time (DEGs-OT); (5) creating Pairwise Correlation Plot for the samples; (6) Time Series Regression Analysis; (7) Determining the pathway, GO (gene ontology) and drug by enrichment analysis. We found that at a 3 h "window period" some specific gene expression may exist after PNI, and responses to lipopolysaccharide (LPS) and TNF signaling pathway may play important roles, suggesting that the inflammatory microenvironment exists after PNI. We also found that troglitazone was closely associated with the change of gene expression after PNI. Therefore, the further evaluation of the precise mechanism of troglitazone on PNI is needed and it may contribute to the development of new drugs for patients with PNI.
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http://dx.doi.org/10.1038/s41598-017-10127-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575162PMC
August 2017

Greatly improved dispersibility of Pt quantum dots in hematite nanoarray and enhanced photoelectrochemical performance.

Nanotechnology 2017 Oct 2;28(41):415603. Epub 2017 Aug 2.

National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, People's Republic of China.

The anchoring of platinum quantum dots (Pt QDs) on the surface of hematite (α-FeO) nanorods is regarded as an efficient way to promote photoelectrochemical activity. To further improve the performance of the Pt-hematite material system, the size and location of the Pt QDs is a key factor to be considered. In this work, an α-FeO nanorod array film was grown on a transparent conductive FTO substrate by a facile hydrothermal method. Pt QDs with a diameter of ∼2 nm were uniformly deposited on the surface of the α-FeO nanorod. The dispersibility of the Pt QDs was greatly improved by regulating the surface wettability of the α-FeO thin film. The dependence of surface wettability on the micro-/nano-structure of the α-FeO array was revealed. Due to the structure regulation of the α-FeO nanoarray and the greatly improved dispersibility of the Pt QDs, the photocurrent of the 2.7 wt% Pt QD anchored α-FeO nanorod array was ten times higher than that of the pure α-FeO nanorod array. This work points to an efficient approach for dispersing the QDs in a nanoarray thin film by adjusting its micro-/nano-structure and surface wettability.
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http://dx.doi.org/10.1088/1361-6528/aa8389DOI Listing
October 2017
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