Publications by authors named "Xiaofeng Han"

93 Publications

Effects of Chiral Molecule Modification on Surface Biosorption Behavior.

Langmuir 2021 04 8;37(15):4441-4448. Epub 2021 Apr 8.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, National Demonstration Center for Experimental, Biomedical Engineering Education, Southeast University, Nanjing 210096, China.

Antifouling materials have many important applications in biomedical devices and marine coating. Oligo(ethylene glycol) (OEG) or poly(ethylene glycol) (PEG) exhibit promising antifouling properties and are widely used in biomedical engineering. Chiral selection is an important phenomenon in biological processes. Because of the influence of steric hindrance, the modification of chiral molecules with different chirality at interfaces will affect the intermolecular interaction at the interfaces and lead to different structures of interfacial molecules. The difference of surface structures such as surface hydration structure would impact the adsorption of biomolecules on the surface, thus causing different varieties of cell adhesion and cell growth. In this study, the influence on surface hydration and surface cell adhesion of OEG self-assembled monolayers (SAMs) modified with cysteine showing different chirality are explored. The water structure at the interfaces of OEG/water in different conditions was probed with sum frequency generation vibrational spectroscopy (SFG-VS). The results show that the interfacial water structure can change significantly with either d-cysteine or l-cysteine modification on OEG. Water molecules are more ordered at the OEG/water interface under the d-cysteine modification on OEG SAMs, which improves the protein adsorption resistance of the surface. In contrast, l-cysteine modification would make the water less ordered at the OEG/protein solution interface and enhance the protein adsorption. Additionally, optical micrographs indicate that l-cysteine can significantly promote the OEG SAMs cell adhesion and growth, while d-cysteine exhibits an inhibitory effect, which is consistent with the results of SFG-VS experiments.
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http://dx.doi.org/10.1021/acs.langmuir.0c03551DOI Listing
April 2021

Volume Deformation of Steam-Cured Concrete with Slag during and after Steam Curing.

Materials (Basel) 2021 Mar 27;14(7). Epub 2021 Mar 27.

School of Civil Engineering, Qingdao University of Technology, No.11 of Fushun Road, Qingdao 266033, China.

In order to better predict the development of shrinkage deformation of steam-cured concrete mixed with slag, a deformation-temperature-humidity integrated model test, a hydration heat test, and an elastic modulus test were performed. The effects of the steam-curing process and the content of slag on shrinkage deformation, hydration degree and elastic modulus of concrete were studied. The results indicate that during the steam-curing process, the concrete has an "expansion-shrinkage" pattern. After the steam curing, the deformation of concrete is dominated by drying shrinkage. After the addition of slag, the shrinkage deformation of steam-cured concrete is increased. The autogenous shrinkage increases by 0.5-12%, and the total shrinkage increases by 1.5-8% at 60 days. At the same time, slag reduces the hydration degree of steam-cured concrete and modulus of elasticity. A prediction model for the hydration degree of steam-cured concrete is established, which can be used to calculate the degree of hydration at any curing age. Based on the capillary tension generated by the capillary pores in concrete, an integrated model of autogenous shrinkage and total shrinkage is established with the relative humidity directly related to the water loss in the concrete as the driving parameter. Whether the shrinkage deformation is caused by hydration reaction or the external environment, this model can better predict the shrinkage deformation of steam-cured concrete.
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http://dx.doi.org/10.3390/ma14071647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037895PMC
March 2021

Prediction of postoperative hematoma occurrence after ultrasound-guided vacuum-assisted breast biopsy in minimally invasive surgery for percutaneous removal of benign breast lesions.

Gland Surg 2020 Oct;9(5):1346-1353

Department of General Surgery, Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing, China.

Background: The exact factors and mechanisms involved in the development of hematoma after breast ultrasound (US)-guided vacuum-assisted biopsy (VAB) are not clear. This study aimed to analyze the occurrence, prognosis, and risk factors of hematoma after US-guided VAB for the removal of benign breast lesions.

Methods: This was a retrospective study of consecutive female patients with breast lesions (BI-RADS 3/4a/4b and confirmed as benign by core needle biopsy) removed by percutaneous excisional biopsy by US-guided VAB at the Beijing Chaoyang Hospital from April 2016 to December 2018. Univariable analyses were conducted to identify the factors associated with hematoma (age, nodule size, laterality, nodule location, number of nodules, BI-RADS classification of ultrasound, pathology, breast shape, menstrual period, efficacy time of bandage, and hemostatic agents). Multivariable analysis was performed to identify the factors independently associated with hematoma.

Results: A total of 293 patients (343 breast lesions) were included. Lesion removal was successful in all cases. Hematomas ≥1 cm were found in 39 patients. Finally, 38 (97.4%) hematomas were absorbed completely within 6 months. The multivariable logistic regression analysis revealed that lesion size (P<0.001; OR: 5.775; 95% CI: 2.752-12.121), number of lesions (P=0.011; OR: 3.205, 95% CI: 1.311-7.834), and the efficacy time of bandage 12-24 h (P=0.003; OR: 5.257, 95% CI: 1.792-15.421) were independently associated with hematoma occurrence after US-guided VAB excision of breast lesions. A lesion size cut-off of 23.4 mm (AUC 0.764, 95% CI: 0.692-0.837; 59.0% sensitivity; 80.3% specificity; 77.5% accuracy; P<0.001) could predict hematoma occurrence.

Conclusions: US-guided excisional VAB can effectively remove benign breast lesions. The factors independently associated with postoperative hematoma are lesion size, lesion number, and effective postoperative compression time of bandage.
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http://dx.doi.org/10.21037/gs-20-344DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667065PMC
October 2020

1,25(OH)2D3 inhibits osteogenic differentiation through activating β‑catenin signaling via downregulating bone morphogenetic protein 2.

Mol Med Rep 2020 Dec 20;22(6):5023-5032. Epub 2020 Oct 20.

Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China.

The present study explored whether bone morphogenetic proteins (BMPs) and Wnt/β‑catenin signaling pathways were involved in the 1,25(OH)2D3‑induced inhibition of osteogenic differentiation in bone marrow‑derived mesenchymal stem cells (BMSCs). To evaluate the osteogenic differentiation of BMSCs, the expression levels of ossification markers, including BMP2, Runt‑related transcription factor 2 (Runx2), Msh homeobox 2 (Msx2), osteopontin (OPN) and osteocalcin (OCN), and the activity of alkaline phosphatase (ALP), as well as the calcified area observed by Alizarin red‑S staining, were investigated. Chromatin immunoprecipitation (ChIP) assay was used to detect the effect of 1,25(OH)2D3 on the DNA methylation and histone modification of BMP2, while an immunoprecipitation (IP) assay was performed to assess the crosstalk between Smad1 and disheveled‑1 (Dvl‑1) proteins. It was observed that 1,25(OH)2D3 significantly decreased the expression levels of BMP2, Runx2, Msx2, OPN and OCN, and reduced ALP activity and the calcified area in BMSCs, whereas these effects were rescued by BMP2 overexpression. ChIP assay revealed that BMSCs treated with 1,25(OH)2D3 exhibited a significant increase in H3K9me2 level and a decrease in the acetylation of histone H3 at the same BMP2 promoter region. In addition, 1,25(OH)2D3 treatment promoted the nuclear accumulation of β‑catenin by downregulating BMP2. Furthermore, the β‑catenin signaling inhibitor XAV‑939 weakened the inhibitory effect of 1,25(OH)2D3 on osteogenic differentiation. Additionally, knockdown of β‑catenin rescued the attenuation in Dvl‑1 and Smad1 interaction caused by 1,25(OH)2D3. Overexpression of Smad1 also reversed the inhibitory effect of 1,25(OH)2D3 on osteogenic differentiation. Taken together, the current study demonstrated that 1,25(OH)2D3 inhibited the differentiation of BMSCs into osteoblast‑like cells by inactivating BMP2 and activating Wnt/β‑catenin signaling.
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http://dx.doi.org/10.3892/mmr.2020.11619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646955PMC
December 2020

Retraction Note to: Explore on the effect of ATF6 on cell growth and apoptosis in cartilage development.

Histochem Cell Biol 2020 08;154(2):245

Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, 400016, China.

The Editor-in-Chief has retracted the article by Han et al. (2014) because Fig. 3a-d are also published as Fig. 5b-e in Liu et al. (2012), and Fig. 3a, c, d are also published as Fig. 5a, d, e in Guo et al. (2014).
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http://dx.doi.org/10.1007/s00418-020-01907-4DOI Listing
August 2020

Measurement of serum intact parathyroid hormone concentration 1 day after total thyroidectomy to assess risk of permanent hypoparathyroidism.

J Int Med Res 2020 Jun;48(6):300060520927199

Department of General Surgery, Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing, China.

Objective: This study was performed to assess the clinical value of measuring the intact parathyroid hormone (iPTH) concentration 1 day after total thyroidectomy to estimate the occurrence of permanent hypoparathyroidism (pHPP).

Methods: Data of 546 patients who underwent total thyroidectomy from February 2008 to December 2018 were retrospectively analyzed. Calcium and iPTH concentrations were collected preoperatively and at 1 day and 6 months postoperatively. Logistic regression was used to analyze the correlation between clinical indexes and postoperative pHPP.

Results: Of the 546 patients, 22 (4.03%) developed pHPP. Multivariate analysis showed that the iPTH and serum calcium concentrations measured 1 day after surgery were independent predictors of the risk of pHPP. An iPTH concentration of 5.51 pg/mL measured 1 day postoperatively was used as the cut-off value, and the area under the curve was 0.956. The risk of pHPP was identified with a sensitivity of 100%, specificity of 85.1%, positive predictive value of 22%, and negative predictive value of 100%.

Conclusions: The iPTH concentration measured 1 day after total thyroidectomy is closely related to the occurrence of pHPP postoperatively and is an independent predictive risk factor. The postoperative iPTH concentration can be helpful in identifying patients at risk for developing pHPP.
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http://dx.doi.org/10.1177/0300060520927199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7294503PMC
June 2020

Four pediatric cases of secondary neoplasms arising in nevus sebaceous.

Dermatol Ther 2020 11 8;33(6):e13762. Epub 2020 Jul 8.

Department of Dermatology, Beijing Children's Hospital, Capital Medical University, National Center For Children's Health, Beijing, China.

Secondary neoplasms of epidermal adnexal origin have been reported to develop into nevus sebaceous (NS), mainly in adulthood but rarely in children. Four cases of secondary neoplasms were identified in 413 children of nevus sebaceous from 2015 to 2019 by our department, accounting for 1% of all cases. We here report the clinical, dermoscopical, and histopathological features of these tumors, including syringocystadenoma papilliferum (SCAP), pilomatricoma, trichilemmoma, and basal cell carcinoma (BCC). We recommend prophylactic excision of nevus sebaceous before puberty, not only because of the cosmetical disfigurement but also due to the risk of malignant transformation.
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http://dx.doi.org/10.1111/dth.13762DOI Listing
November 2020

Dynamics analysis of a delayed virus model with two different transmission methods and treatments.

Adv Differ Equ 2020 6;2020(1). Epub 2020 Jan 6.

1College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, P.R. China.

In this paper, a delayed virus model with two different transmission methods and treatments is investigated. This model is a time-delayed version of the model in (Zhang et al. in Comput. Math. Methods Med. 2015:758362, 2015). We show that the virus-free equilibrium is locally asymptotically stable if the basic reproduction number is smaller than one, and by regarding the time delay as a bifurcation parameter, the existence of local Hopf bifurcation is investigated. The results show that time delay can change the stability of the endemic equilibrium. Finally, we give some numerical simulations to illustrate the theoretical findings.
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http://dx.doi.org/10.1186/s13662-019-2438-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100214PMC
January 2020

Computed tomography-based hemodynamic index for aortic dissection.

J Thorac Cardiovasc Surg 2020 Feb 20. Epub 2020 Feb 20.

Department of Biomedical Engineering, School of Life Science, Beijing Institute of Technology, Beijing, China. Electronic address:

Objective: In this study we aimed to propose a new computed tomography-based hemodynamic indicator to quantify the functional significance of aortic dissection and predict post intervention luminal remodeling.

Methods: Computational hemodynamics and 3D structural analyses were conducted in 51 patients with type B aortic dissection, at initial presentation and at approximately 1 month, 3 months, and 1 year post intervention. A functional index was proposed on the basis of luminal pressure difference. Statistical relationships between the proposed indicator and longitudinal luminal development were analyzed.

Results: The computed luminal pressure difference (true lumen pressure minus false lumen pressure) varied overall from positive to negative along the aorta. The first balance position at which the pressure difference equals 0 was proposed as the functional indicator. A more distally located first balance position indicated better functional status. Implantation of stent graft distally shifted this balance position. Patients with the balance position shifted out of the dissected region (43%) presented the highest functional improvement after intervention; whereas those with the balance position shifted to the abdominal region (25%) showed unsatisfactory results. The magnitude of distal shifting of the first balance position at 3 months post intervention was statistically related to the subsequent true lumen expansion and false lumen reduction.

Conclusions: The first balance position of luminal pressure difference quantified the hemodynamic status of the dissected aorta. The magnitude of distal shifting of the balance position after intervention was associated with functional improvement and might be used predict longitudinal aortic remodeling.
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http://dx.doi.org/10.1016/j.jtcvs.2020.02.034DOI Listing
February 2020

Analysis of drivers' deceleration behavior based on naturalistic driving data.

Traffic Inj Prev 2020 27;21(1):42-47. Epub 2020 Jan 27.

State Key Laboratory of Automotive Safety & Energy, School of Vehicle and Mobility, Tsinghua University, Beijing, China.

As one of the bases for designing a humanlike brake control system for the intelligent vehicle, drivers' deceleration behavior needs to be understood. There are two modes for drivers' deceleration behavior: (i) brake pedal input, by applying brake system to reduce the speed; (ii) no pedal input, by releasing the accelerator pedal without pressing the brake pedal, thus decelerating by naturalistic driving resistance. The deceleration behavior that drivers choose to press the brake pedal has been investigated in previous studies. However, releasing the accelerator pedal behavior has not received much attention. The objective of this study is to investigate factors that influence drivers' choice of the two deceleration modes using naturalistic driving data, which provide a theoretical foundation for the design of the brake control system. A logistic model was constructed to model drivers' deceleration mode, valued as "no pedal input" or "brake pedal input" for dependent variables. Factors such as Light condition, Intersection mode, Road alignment, Traffic flow, Traffic light, Ego-vehicle motion state, Lead vehicle motion state, Time headway (THW), and Ego-vehicle speed were considered in the model as independent variables. 393 deceleration events were selected from the naturalistic driving data, which used as the database for the regression model. As a result, 6 remarkable factors were found to influence drivers' deceleration model, which include Traffic flow, Intersection mode, Lead vehicle motion state, Ego-vehicle motion state, Ego-vehicle speed and THW. Specifically, (1) the possibility of drivers choosing "no pedal input" is gradually increasing with the increase of THW and speed; (2) The drivers prefer to choose "no pedal input" when the lead vehicle is decelerating compared to it's stationary. This probability is relatively high when the lead vehicle is traveling along the road; (3) the possibility of choosing "no pedal input" at intersection is higher than roads without intersection; (4) the possibility of choosing "no pedal input" is higher when traveling with more traffic flow. The drivers' deceleration behavior can be divided into "no pedal input" and "brake pedal input." The following six factors significantly affect drivers' choice of deceleration mode: Traffic flow, Intersection mode, Lead vehicle motion state, Ego-vehicle motion state, Ego-vehicle speed and THW. The logistic regression model can quantify the influence of these six factors on drivers' deceleration behavior. This study provides a theoretical basis for the braking system design of ADAS (Advanced Driving Assistant System) and intelligent control system.
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http://dx.doi.org/10.1080/15389588.2019.1707194DOI Listing
December 2020

Revealing Molecular-Level Interaction between a Polymeric Drug and Model Membrane Via Sum Frequency Generation and Microfluidics.

Langmuir 2020 02 10;36(6):1615-1622. Epub 2020 Feb 10.

Department of Biomedical Engineering , Southeast University , Jiangsu 210096 , China.

Body fluids flow all over the body and affect the biological processes at biointerfaces. To simulate such a case, sum frequency generation (SFG) vibrational spectroscopy and a self-designed microfluidic chip were combined together to investigate the interaction between a pH-responsive polymeric drug, poly(α-propylacrylic acid) (PPAAc), and the model cell membranes in different liquid environments. By examining the SFG spectra under the static and flowing conditions, the drug-membrane interaction was revealed comprehensively. The interfacial water layer was screened as the key factor affecting the drug-membrane interaction. The interfacial water layer can prevent the side propyl groups on PPAAc from inserting into the model cell membrane but would be disrupted by numerous ions in buffer solutions. Without flowing, at pH 6.6, the interaction between PPAAc and the model cell membrane was strongest; with flowing, at pH 5.8, the interaction was strongest. Flowing was proven to substantially affect the interaction between PPAAc and the model cell membranes, suggesting that the fluid environment was of key significance for biointerfaces. This work demonstrated that, by combining SFG and microfluidics, new information about the molecular-level interaction between macromolecules and the model cell membranes can be acquired, which cannot be obtained by collecting the normal static SFG spectra.
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http://dx.doi.org/10.1021/acs.langmuir.9b03676DOI Listing
February 2020

Calcium-dependent and -independent annexin V binding: distinct molecular behaviours at cell membrane interfaces.

Chem Commun (Camb) 2020 Feb;56(11):1653-1656

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Distinct annexin V binding behaviours in Ca2+-dependent and Ca2+-independent cases were comparatively investigated using sum frequency generation vibrational spectroscopy. It was discovered that binding affected the molecular arrangement of both membrane leaflets, and the initial Ca2+-independent binding went through a transition with annexin V reorientation to a more stable state upon adding Ca2+.
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http://dx.doi.org/10.1039/c9cc09184jDOI Listing
February 2020

Corrigendum to "Plasma membrane activatable polymeric nanotheranostics with self-enhanced light-triggered photosensitizer cell influx for photodynamic cancer therapy" [J. Control. Release 255 (2017) 231-241].

J Control Release 2020 Feb 23;318:232-233. Epub 2019 Dec 23.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. Electronic address:

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http://dx.doi.org/10.1016/j.jconrel.2019.12.008DOI Listing
February 2020

Harnessing tumor-associated macrophages as aids for cancer immunotherapy.

Mol Cancer 2019 12 5;18(1):177. Epub 2019 Dec 5.

The First Affiliated Hospital of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Institutes for Translational Medicine, Soochow University Medical College, 199 Renai Road, Suzhou, 215123, Jiangsu, China.

Cancer immunotherapies that engage immune cells to fight against tumors are proving to be powerful weapons in combating cancer and are becoming increasingly utilized in the clinics. However, for the majority of patients with solid tumors, little or no progress has been seen, presumably due to lack of adequate approaches that can reprogram the local immunosuppressive tumor milieu and thus reinvigorate antitumor immunity. Tumor-associated macrophages (TAMs), which abundantly infiltrate most solid tumors, could contribute to tumor progression by stimulating proliferation, angiogenesis, metastasis, and by providing a barrier against antitumor immunity. Initial TAMs-targeting strategies have shown efficacy across therapeutic modalities and tumor types in both preclinical and clinical studies. TAMs-targeted therapeutic approaches can be roughly divided into those that deplete TAMs and those that modulate TAMs activities. We here reviewed the mechanisms by which macrophages become immunosuppressive and compromise antitumor immunity. TAMs-focused therapeutic strategies are also summarized.
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http://dx.doi.org/10.1186/s12943-019-1102-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894344PMC
December 2019

Ethanol extract of Gynura bicolor (GB) protects against UVB-induced photodamage of skin by inhibiting P53-mediated Bcl-2/BAX/Caspase-3 apoptosis pathway.

Arch Dermatol Res 2020 Jan 19;312(1):41-49. Epub 2019 Sep 19.

Dermatology, Chongqing Traditional Chinese Medicine Hospital, Chongqing First People's Hospital, No. 40, Yuzhong District, Chongqing, 400011, China.

To investigate the protective effect of ethanol extract of Gynura bicolor (GB) against UVB-induced photodamage of skin and the possible mechanisms. DPPH (1,1-diphenyl-2-pico radical) test was used to detect the antioxidant capacity of ethanol extract of Gynura bicolor (GB). The protective effects of GB against UVB irritation were detected both in Hacat cells and photodamage rat models. UVB irradiation could inhibit viability and induce apoptosis of Hacat cells in a dose-dependent manner. The pretreatment of Hacat cells by GB could obviously reverse the effects in a dose-dependent manner. The mRNA and protein expressions of p53, Bax, caspase-3 were increased, while anti-apoptotic protein Bcl-2 was decreased and this effect could be reversed by GB pretreatment in a dose-dependent manner. In vivo, the application of GB could alleviate the skin damage of SD rats and improve the superficial inflammation of the dermis as well as inhibit the expressions of P53 and Caspase-3 induced by UVB irradiation. Ethanol extract of Gynura bicolor could protect the photodamage of human Hacat keratinocytes and SD rats against UVB irradiation by inhibiting P53-mediated Bcl-2/ BAX/Casaspe-3 apoptosis pathway.
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http://dx.doi.org/10.1007/s00403-019-01977-yDOI Listing
January 2020

Origin identification of Chinese Maca using electronic nose coupled with GC-MS.

Sci Rep 2019 08 21;9(1):12216. Epub 2019 Aug 21.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.

Maca (Lepidium meyenii Walp.), originated in the high Andes of Peru, is rich in nutrients and phytochemicals. As a new resource food in China, Maca suffers marketing disorders due to the limitation of basic research. Due to the close relationship of Maca quality and origin of place, it's of scientific, economic and social importance to set up a rapid, reliable and efficient method to identify Maca origin. In the present study, 303 Maca samples were collected from 101 villages of the main producing area in China. Using electronic nose and BP neutral network algorithm, a Maca odor database was set up to trace the origin. GC-MS was then employed to analyze the characteristic components qualitatively and semi-quantitatively. As a result, very significant differences (p < 0.01) were detected in the volatile components of Maca from different areas. This study not only constructs a network model to forecast the Maca origin, but also reveals the relationship between Maca odor fingerprints and origins.
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http://dx.doi.org/10.1038/s41598-019-47571-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6704143PMC
August 2019

Long non-coding RNA IQCJ-SCHIP1 antisense RNA 1 is downregulated in colorectal cancer and inhibits cell proliferation.

Ann Transl Med 2019 May;7(9):198

Wuxi Cancer Institute, Affiliated Hospital of Jiangnan University, Wuxi 214062, China.

Background: IQCJ-SCHIP1 antisense RNA 1 (IQCJ-SCHIP1-AS1) was a functional novel long non-coding RNA (lncRNA) revealed by our previous expression profile. In this study, we aim to investigate its clinical relevance and biological significance in colorectal cancer (CRC).

Methods: We measured the expression levels of IQCJ-SCHIP1-AS1 in 86 paired CRC tissues using quantitative RT-PCR assay, and then analyzed its association with patient prognoses. Moreover, gain-of-function and loss-of-function studies were performed to examine the biological functions of IQCJ-SCHIP1-AS1. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA) were used to elucidate potential mechanisms of IQCJ-SCHIP1-AS1 in CRC.

Results: More than 2-fold decreased expression of IQCJ-SCHIP1-AS1 was found in half of CRC tissues (53.5%, 46/86). IQCJ-SCHIP1-AS1 down-regulation was correlated with poor differentiation (P=0.025), advanced depth of tumor (P=0.022), lymphatic invasion (P=0.010), advanced tumor stage (P=0.006), and poor prognosis (P=0.0027) in CRC patients. The Cox proportional hazards model demonstrated that IQCJ-SCHIP1-AS1 expression was an independent prognostic factor for CRC (HR =0.247, 95% CI: 0.081-0.752, P=0.014). Moreover, knockdown of IQCJ-SCHIP1-AS1 promoted CRC cell proliferation through increasing cell cycle progression and impairing cell apoptosis. Additionally, bioinformatics analysis showed that differential expression genes in IQCJ-SCHIP1-AS1-depleted CRC cells were enriched in the pathways of cell cycle, DNA replication, and p53.

Conclusions: Our results demonstrate that IQCJ-SCHIP1-AS1 has an indicative tumor suppressor role and appears to be a potential prognostic factor in CRC for the first time.
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http://dx.doi.org/10.21037/atm.2019.04.21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6545302PMC
May 2019

Treatment of Café-Au-Lait Spots Using Q-Switched Alexandrite Laser: Analysis of Clinical Characteristics of 471 Children in Mainland China.

Lasers Surg Med 2019 10 25;51(8):694-700. Epub 2019 May 25.

Department of Dermatology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 100045, Beijing, China.

Background And Objectives: Café-au-lait spots, also known as café-au-lait macules (CALMs), are a common pigmentary disorder. Although various laser modalities have been used to treat CALMs, the efficacy of laser treatment in children differs from that in adults. We investigated the efficacy, safety, and clinical factors of the treatment of CALMs using Q-switched alexandrite laser (755 nm) therapy in children.

Methods: In total, 471 children with CALMs underwent Q-switched alexandrite laser therapy at a treatment interval of 3-12 months. The safety and efficacy of the laser treatment were evaluated by reviewing clinical records and photographs before and after treatments.

Results: Of the 471 patients, 140 (29.72%) were cured completely, 124 (26.33%) showed substantial improvement, 110 (23.35%) showed improvement, and 97 (20.60%) showed no improvement after one to nine treatments. The overall treatment success rate was 79.41%, and the treatment efficacy was positively correlated with the number of laser treatments (rs = 0.26, P < 0.0001). Sex and the interval of laser treatments were also associated with significant differences in treatment outcomes (P < 0.05). No obvious adverse effects were observed. Multivariate logistic regression analysis showed that the number of treatments influenced the treatment efficacy (odds ratio, 2.130; 95% confidence interval, 1.561-2.908).

Conclusions: Q-switched alexandrite laser (755 nm) therapy is safe and highly effective for CALMs in children, and the number of treatments affects the treatment efficacy. Lasers Surg. Med. © 2019 The Authors. Lasers in Surgery and Medicine Published by Wiley Periodicals, Inc.
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http://dx.doi.org/10.1002/lsm.23097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6771696PMC
October 2019

Research on Fault Extraction Method of Variational Mode Decomposition Based on Immunized Fruit Fly Optimization Algorithm.

Entropy (Basel) 2019 Apr 15;21(4). Epub 2019 Apr 15.

School of Mechanical Engineering, North University of China, Taiyuan 030051, China.

In recent years, a new method of fault diagnosis, named variational mode decomposition (VMD), has been widely used in industrial production, but the decomposition accuracy of VMD is determined by two parameters, which are respectively the decomposition layer number k and the penalty factor α, if the parameters are not properly selected, there will be over-decomposition or under-decomposition. In order to find an approach to determine the parameters adaptively, a method to optimize VMD by using the immune fruit fly optimization algorithm (IFOA) is proposed in this paper. In this method, permutation entropy is used as the fitness function, firstly, the immune fruit fly optimization algorithm is used to search the combined parameters of k and α in VMD, searching for the best combination parameters of k and α by iteration, and then uses the combined parameters to perform VMD, finally, the center frequency is determined through frequency spectrum analysis. The method mentioned is applied to the fault extraction of a simulated signal and a measured signal of a wind turbine gearbox, and the fault frequency is successfully extracted. Using ensemble empirical mode decomposition (EEMD) and singular spectrum decomposition (SSD) to compare with the proposed method, which validated feasibility of the proposed method.
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http://dx.doi.org/10.3390/e21040400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7514893PMC
April 2019

Optimization schemes for endovascular repair with parallel technique based on hemodynamic analyses.

Int J Numer Method Biomed Eng 2019 06 1;35(6):e3197. Epub 2019 Apr 1.

Department of Vascular and Endovascular Surgery, Chinese PLA General Hospital, Beijing, China.

Endovascular repair with parallel stent-grafts (SG) is a challenging technique that reconstructs the luminal flow pathways by implanting parallel-placed SGs into the vessel. After treatment, occlusion and shifting of the parallel SGs are sometimes reported, which could be fatal and difficult to be re-operated. These issues are highly related to the local hemodynamic conditions in the stented region. In this study, a patient case treated by the octopus endograft technique (a head-SG with three limb-SGs) and experienced limb-SG occlusion is studied. 3-D models are established based on computed tomography (CT) angiography datasets pretreatment and posttreatment as well as during follow-ups. Hemodynamic quantities such as pressure drop, wall shear stress-related parameters, and flow division in limb-SGs and visceral arteries are quantitatively investigated. Optimizations on the length of the head-SG and diameter of the limb-SGs are analyzed based on various scenarios. The results indicate that when reconstructing the flow pathways via octopus stenting, it is important to ensure the flow distribution as physiologically required with this new morphology. Position (or length) of the head-SG and diameter of the limb-SGs play an important role in controlling flow division, and high time average wall shear stress (TAWSS) around the head-SG acts as a main factor for graft immigration. This study, by proposing optimization suggestions with hemodynamic analyses for a specific case, implicates that pretreatment SG scenarios may assist in wise selection and placement of the device and thus may improve long-term effectiveness of this kind of challenging endovascular repair techniques.
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http://dx.doi.org/10.1002/cnm.3197DOI Listing
June 2019

Modified Singular Spectrum Decomposition and Its Application to Composite Fault Diagnosis of Gearboxes.

Sensors (Basel) 2018 Dec 24;19(1). Epub 2018 Dec 24.

College of Mechanical Engineering, North University of China, Taiyuan 030051, China.

Under the strong noise environment, the composite fault signal of gearbox is weak, which makes it difficult to extract fault features. For this problem, based on noise-assisted method, we propose a novel method called Modified Singular Spectrum Decomposition (MSSD). Singular Spectrum Decomposition (SSD) has many advantages such as high decomposition precision and strong ability to restrain mode mixing, etc. However, the ability of SSD to extract a weak signal is not ideal, the decomposition results usually contain a lot of redundant noise and mode mixing caused by intermittency, which is also a troubling problem. In order to improve the decomposition efficiency and make up for the defects of SSD, the new method MSSD adds an adaptive and particular noise in every SSD decomposition stage for each trial, and in addition, whenever the input signal is decomposed to obtain an intrinsic module function (IMF), a unique residual is obtained. After multiple decomposition, the average value of the residual is used as input to the next stage, until the residual cannot continue to decompose, which means that the residual component has, at most, one extreme value. Finally, analyzing simulated signals to explain the advantages of MSSD compared to ensemble empirical mode decomposition (EEMD) and complete ensemble local mean decomposition with adaptive noise (CEEMDAN). In order to further prove the effectiveness of MSSD, this new method, MSSD, is applied to the fault diagnosis of an engineering gearbox test stand in an actual engineer project case. The final results show that MSSD can extract more fault feature information, and mode mixing has been improved and suffers less interference compared to SSD.
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http://dx.doi.org/10.3390/s19010062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339200PMC
December 2018

Virtual stenting with simplex mesh and mechanical contact analysis for real-time planning of thoracic endovascular aortic repair.

Theranostics 2018 10;8(20):5758-5771. Epub 2018 Nov 10.

School of Life Science, Beijing Institute of Technology, Beijing 100081, China.

In aortic endovascular repair, the prediction of stented vessel remodeling informs treatment plans and risk evaluation; however, there are no highly accurate and efficient methods to quantitatively simulate stented vessels. This study developed a fast virtual stenting algorithm to simulate stent-induced aortic remodeling to assist in real-time thoracic endovascular aortic repair planning. The virtual stenting algorithm was established based on simplex deformable mesh and mechanical contact analysis. The key parameters of the mechanical contact analysis were derived from mechanical tests on aortic tissue (n=40) and commonly used stent-grafts (n=6). Genetic algorithm was applied to select weighting parameters. Testing and validation of the algorithm were performed using pre- and post-treatment computed tomography angiography datasets of type-B aortic dissection cases (n=66). The algorithm was efficient in simulating stent-induced aortic deformation (mean computing time on a single processor: 13.78±2.80s) and accurate at the morphological (curvature difference: 1.57±0.57%; cross-sectional area difference: 4.11±0.85%) and hemodynamic (similarity of wall shear stress-derived parameters: 90.16-90.94%) levels. Stent-induced wall deformation was higher (p<0.05) in distal stent-induced new entry cases than in successfully treated cases, and this deformation did not differ significantly among the different stent groups. Additionally, the high stent-induced wall deformation regions and the new-entry sites overlapped, indicating the usefulness of wall deformation to evaluate the risks of device-induced complications. The novel algorithm provided fast real-time and accurate predictions of stent-graft deployment with luminal deformation tracking, thereby potentially informing individualized stenting planning and improving endovascular aortic repair outcomes. Large, multicenter studies are warranted to extend the algorithm validation and determine stress-induced wall deformation cutoff values for the risk stratification of particular complications.
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http://dx.doi.org/10.7150/thno.28944DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276306PMC
September 2019

Absolute Orientations of Water Molecules at Zwitterionic Polymer Interfaces and Interfacial Dynamics after Salt Exposure.

Langmuir 2019 02 4;35(5):1327-1334. Epub 2018 Dec 4.

Department of Chemistry , University of Michigan , Ann Arbor , Michigan 48109 , United States.

Nonfouling zwitterionic polymers have wide applications ranging from the naval industry to biomedical engineering. Strong hydration at polymer surfaces has been proven to be crucial to their nonfouling property, but the absolute orientations of water molecules on the polymers and the competition between water and salt binding have not been elucidated. In this work, the absolute orientations of water molecules on two zwitterionic polymer brushes, poly(carboxybetaine methacrylate) (pCBMA) and poly(sulfobetaine methacrylate) (pSBMA), were measured using regular and phase-sensitive sum frequency generation (SFG) vibrational spectroscopy. The pH-dependent studies in a pH range from 2 to 12 showed that at a pH of 7, the water absolute orientations are different on the pCBMS and pSBMA surfaces. Phase-sensitive SFG studies confirmed the results obtained from the pH-dependent measurements. Salt effects on the hydration of zwitterionic polymers were examined as a function of time, which indicated that the pCBMA surface and the associated interfacial water exhibit a slow restructuring process after salt binding (likely due to the strong binding of pCBMA with water), whereas the surface of pSBMA and the associated water have a fast change after salt binding.
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http://dx.doi.org/10.1021/acs.langmuir.8b01515DOI Listing
February 2019

Positive non-sentinel axillary lymph nodes in breast cancer with 1-2 sentinel lymph node metastases.

Medicine (Baltimore) 2018 Nov;97(44):e13015

Department of General Surgery, Beijing Chaoyang Hospital Affiliated with Capital Medical University, Beijing, China.

Recent clinical trials have shown that sentinel lymph node biopsy (SLNB) alone without axillary lymph node dissection (ALND) can offer excellent regional control if there is sentinel lymph nodes (SLN) metastases to 1-2 nodes. This study aimed to explore the predictive factors for non-sentinel lymph node (NSLN) metastasis in breast cancer patients with 1-2 positive SLNs.Patients with breast cancer and 1-2 positive SLN admitted between March 2009 and March 2017 and who underwent ALND after SLN biopsy (SLNB) at Beijing Chaoyang Hospital were analyzed retrospectively. Factors influencing the status of NSLN were studied by univariate and multivariate analysis.Of 1125 patients, 147 patients had SLN metastasis (13.1%) and 119 patients (81.0%) had 1-2 positive SLNs. Among them, 42 patients (35.3%) had NSLN metastasis. The invasive tumor size (P <.001), histological grade (P =.011), lymphovascular invasion (LVI) (P =.006), and over-expression of HER2 (P =.025) significantly correlated with non-SLN metastasis by univariate analysis. LVI (LVI) (P =.007; OR: 4.130; 95% confidence interval [CI]: 1.465-11.641), invasive tumor size (P <.001; OR: 7.176; 95% CI: 2.710-19.002), and HER2 over-expression (P =.006; OR: 5.489; 95% CI: 1.635-18.428) were independently associated with NSLN metastasis by the Logistic regression model. The ROC analysis identified a cut-off point of 26 mm of tumor size (area under the receiver operating characteristic [ROC] curve [AUC] 0.712, CI: 0.614-0.811) was useful for dividing patients with positive SLN (1-2 nodes) into non-SLN-positive and non-SLN-negative groups.For 1-2 positive SLNs of breast cancer, LVI, large invasive tumor size, and HER2 over-expression are independent factors affecting NSLN metastases.
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http://dx.doi.org/10.1097/MD.0000000000013015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6221619PMC
November 2018

Molecular Coupling between Organic Molecules and Metal.

J Phys Chem Lett 2018 Sep 28;9(17):5167-5172. Epub 2018 Aug 28.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , Nanjing 210096 , China.

Molecular couplings at interfaces play important roles in determining the performance of nanophotonics and molecular electronics. In this Letter, using femtosecond sum frequency generation to trace free-induction decay of vibrationally excited aromatic thiol molecules immobilized on metal with and without the bridged methylene group(s), metal surface free electron-coupled and uncoupled phenyl C-H stretching vibrational modes were identified, with dephasing times of ∼0.28 and ∼0.60 ps, respectively. For thiols on Au with the bridged methylene group(s) (benzyl mercaptan and phenylethanethiol), both the coupled and uncoupled modes were observed; for thiol on Au without the bridged methylene group (thiophenol), only the coupled mode was observed. This indicates that the bridged methylene group(s) serving as a spacer can be used to adjust the molecular coupling between the phenyl vibration and surface free electrons. The experimental approach can be used to tune molecular couplings in advanced nanophotonics and molecular electronics.
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http://dx.doi.org/10.1021/acs.jpclett.8b01765DOI Listing
September 2018

Long non-coding RNA HOXA-AS2 promotes migration and invasion by acting as a ceRNA of miR-520c-3p in osteosarcoma cells.

Cell Cycle 2018 6;17(13):1637-1648. Epub 2018 Aug 6.

a Department of Orthopaedics, Renji Hospital , Shanghai Jiao Tong University School of Medicine , Shanghai , P.R. China.

Osteosarcoma (OS) is the commonest primary malignant tumour originating from bone. Previous studies demonstrated that long non-coding RNAs (lncRNAs) could participate in both oncogenic and tumor suppressing pathways in various cancer, including OS. The HOXA cluster antisense RNA2 (HOXA-AS2) plays an important role in carcinogenesis, however, the underlying role of HOXA-AS2 in OS progression remains unknown. The aim of the present study was to evaluate the expression and function of HOXA-AS2 in OS. The qRT-PCR analysis was to investigate the expression pattern of HOXA-AS2 in OS tissues. Then, the effects of HOXA-AS2 on cell proliferation, cell cycle, apoptosis, migration, and invasion were assessed in OS in vitro. Furthermore, bioinformatics online programs predicted and luciferase reporter assay were used to validate the association of HOXA-AS2 and miR-520c-3p in OS cells. We observed that HOXA-AS2 was up-regulated in OS tissues. In vitro experiments revealed that HOXA-AS2 knockdown significantly inhibited OS cells proliferation by promoting apoptosis and causing G1 arrest, whereas HOXA-AS2 overexpression promoted cell proliferation. Further functional assays indicated that HOXA-AS2 significantly promoted OS cell migration and invasion by promoting epithelial-mesenchymal transition (EMT). Bioinformatics online programs predicted that HOXA-AS2 sponge miR-520c-3p at 3'-UTR with complementary binding sites, which was validated using luciferase reporter assay. HOXA-AS2 could negatively regulate the expression of miR-520c-3p in OS cells. In conclusion, our study suggests that HOXA-AS2 acts as a functional oncogene in OS.
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http://dx.doi.org/10.1080/15384101.2018.1489174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133314PMC
November 2019

miRNA-29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β-catenin signaling pathways.

Oncol Lett 2018 Aug 5;16(2):2638-2644. Epub 2018 Jun 5.

Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, P.R. China.

In the present study, the effects of microRNA-29a (miRNA-29a) on colon cancer cell viability and the molecular mechanisms underlying the effects were investigated. The expression of miRNA-29a in colon cancer serum samples was notably downregulated, compared with in the normal group. First, miRNA-29a mimic was used to increase the expression of miRNA-29a in HCT-116 cells. Furthermore, upregulation of miRNA-29a suppressed cell viability, increased lactate dehydrogenase levels and apoptosis, and promoted caspase-3/9 activities and B-cell lymphoma 2-associated X protein and phosphatase and tensin homolog (PTEN) protein expression in colon cancer cells. Furthermore, upregulation of miRNA-29a decreased phosphoinositide 3-kinase, phosphorylated (p)-protein kinase B (Akt) and p-glycogen synthase kinase 3β (GSK3β) protein expression and suppressed the Wnt/β-catenin signaling pathway in colon cancer cells. The results of the present study verified that the protective effects of miRNA-29a suppress the PTEN/Akt/GSK3β and Wnt/β-catenin signaling pathways in colon cancer.
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http://dx.doi.org/10.3892/ol.2018.8905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6036559PMC
August 2018

Dynamics Analysis of a Nonlinear Stochastic SEIR Epidemic System with Varying Population Size.

Entropy (Basel) 2018 May 17;20(5). Epub 2018 May 17.

College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao 266590, China.

This paper considers a stochastic susceptible exposed infectious recovered (SEIR) epidemic model with varying population size and vaccination. We aim to study the global dynamics of the reduced nonlinear stochastic proportional differential system. We first investigate the existence and uniqueness of global positive solution of the stochastic system. Then the sufficient conditions for the extinction and permanence in mean of the infectious disease are obtained. Furthermore, we prove that the solution of the stochastic system has a unique ergodic stationary distribution under appropriate conditions. Finally, the discussion and numerical simulation are given to demonstrate the obtained results.
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http://dx.doi.org/10.3390/e20050376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7512895PMC
May 2018

Investigation on nutritional risk assessment and nutritional support status of surgical patients with colorectal cancer.

J BUON 2018 Jan-Feb;23(1):62-67

Department of General Surgery, Jingxi Campus of Beijing Chaoyang Hospital, Affiliated Capital Medical University, Beijing, China.

Purpose: To investigate the malnutrition, the incidence of nutritional risk and the application of nutritional support for patients with colorectal cancer, so as to provide a basis for the rational clinical application of nutritional support.

Methods: A total of 264 surgical patients with colorectal cancer treated/followed up from January 2016 to March 2017 in Beijing Chaoyang Hospital were selected. The nutritional risk was assessed using the Nutritional Risk Screening 2002 (NRS2002), and the nutritional risk and application of nutritional support for patients with different gender, age and clinical disease stage were analyzed; the hospitalization duration and postoperative complications were also analyzed and compared.

Results: According to NRS2002, malnutrition accounted for 6.06% and nutrition risk accounted for 79.55%. There was no statistically significant difference in the nutritional risk between patients with different gender (p=0.059), the nutritional risk of the elderly patients (≥60 years) was higher than that of the non-elderly patients (<60 years) (p<0.001), and the nutritional risk among patients with different clinical stages had no statistically significant difference (p=0.654). All patients received nutritional support; the parenteral nutrition (PN) support rate was 39.02%, while PN+enteral nutrition (EN) support rate was 60.98%, while there was no patient receiving complete EN support. There was no statistically significant difference in the incidence rates of postoperative complications between patients with and without nutritional risk (p=0.546), but there was a statistically significant difference in the hospitalization duration between patients with and without nutritional risk (p=0.019).

Conclusion: The incidence rates of malnutrition and nutritional risk are high in patients with colorectal cancer and the incidence of nutritional risk is related to age. The application of NRS2002 in nutritional risk screening for patients with colorectal cancer can provide a reasonable and effective basis for the clinical nutritional support.
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January 2019

Observing different dynamic behaviors of weakly and strongly adsorbed polystyrene chains at interfaces.

Soft Matter 2018 Apr;14(15):2762-2766

State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, Jiangsu Province, P. R. China.

Understanding the dynamic behavior of polymer chains adsorbed onto a solid surface is of great importance for elucidating polymer-surface interactions. Here by using sum frequency generation (SFG) vibrational spectroscopy, we probed the conformational changes of weakly adsorbed and strongly adsorbed polystyrene (PS) chains on sapphire surfaces perturbed by a nonsolvent (deuterated water, D2O) and a good solvent (carbon tetrachloride, CCl4). The SFG results indicated that the PS chains in the weakly adsorbed state were flexible and the chain conformation could easily be altered. Differently, the PS chains in the strongly adsorbed state were rigid and the chain conformation could not be changed. The local structural variations of the weakly and strongly adsorbed PS chains at the interfaces were also discussed in detail with respect to the SFG spectral characteristics. These intriguing experimental results shed light on our understanding of molecular behaviors of polymer chains adsorbed onto a solid surface.
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http://dx.doi.org/10.1039/c7sm02503cDOI Listing
April 2018