Publications by authors named "Xiaofei Zhang"

561 Publications

TRABID targets DDB2 for deubiquitination to promote proliferation of hepatocellular carcinoma cells.

Biochem Biophys Res Commun 2022 Aug 2;625:23-30. Epub 2022 Aug 2.

CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China; University of Chinese Academy of Sciences, Beijing, China. Electronic address:

TRAF-binding domain-containing protein (TRABID), a member of the OTU deubiquitinase family, has an important role in regulating cellular functions via deubiquitinating substrate proteins such as EZH2 and Jmjd2d. However, the mechanism of its role in the proliferation of hepatocellular carcinoma (HCC) cells has not been fully elucidated. Here, we analyzed the interactome of TRABID in HepG2 cells through mass spectrometry-based proteomics and found that TRABID is associated with damaged DNA-binding protein2 (DDB2). Immunoprecipitation assay showed that the interaction of TRABID and DDB2 is mediated by their OTU domain and N-terminal region, respectively. Furthermore, TRABID deubiquitinates DDB2, and this deubiquitination effect of TRABID depends on its active site. Functionally, we showed that TRABID-mediated hepatocellular carcinoma cell proliferation is attenuated by DDB2 knockdown. Thus, our data revealed a critical role of the TRABID-DDB2 axis in the proliferation of hepatocellular carcinoma cells.
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http://dx.doi.org/10.1016/j.bbrc.2022.07.109DOI Listing
August 2022

miR-3934 regulates the apoptosis and secretion of inflammatory cytokines of basophils via targeting RAGE in asthma.

Allergy Asthma Clin Immunol 2022 Aug 4;18(1):66. Epub 2022 Aug 4.

Department of Neurobiology, Harbin Medical University, Heilongjiang, China.

Background: Several miRNAs are now known to have clear connections to the pathogenesis of asthma. The present study focused on the potential role of miR-3934 during asthma development.

Methods: miR-3934 was detected as a down-regulated miRNA in basophils by sequencing analysis. Next, the expression levels of miR-3934 in peripheral blood mononuclear cells of 50 asthma patients and 50 healthy volunteers were examined by RT-qPCR methods. The basophils were then treated with AGEs and transfected with miR-3934 mimics. The apoptosis levels were examined by flow cytometry assay; and the expression levels of cytokines were detected using the ELISA kits. Finally, the Western blot was performed to examined the expression of key molecules in the TGF-β/Smad signaling pathway.

Results: miR-3934 was down-regulated in the basophils of asthmatic patients. The expression of the pro-inflammatory cytokines IL-6, IL-8 and IL-33 was enhanced in basophils from asthmatic patients, and this effect was partially reversed by transfection of miR-3934 mimics. Furthermore, receiver operating characteristics analysis showed that miR-3934 levels can be used to distinguish asthma patients from healthy individuals. miR-3934 partially inhibited advanced glycation end products-induced increases in basophil apoptosis by suppressing expression of RAGE.

Conclusion: Our results indicate that miR-3934 acts to mitigate the pathogenesis of asthma by targeting RAGE and suppressing TGF-β/Smad signaling.
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http://dx.doi.org/10.1186/s13223-022-00704-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354354PMC
August 2022

Study of the Mechanism of Antiemetic Effect of Mill. Essential Oil Based on Ca/CaMKII/ERK1/2 Pathway.

Drug Des Devel Ther 2022 26;16:2407-2422. Epub 2022 Jul 26.

Department of Pharmaceutics, The Key Laboratory of Basic and New Drug Research of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang, People's Republic of China.

Purpose: To investigate the effective components and possible mechanism of action of Mill. essential oil (LEO) in preventing vomiting through the olfactory pathway.

Materials And Methods: A new network pharmacology-based method was established to analyze main components and pathways of LEO involved in antiemetic effects by introducing component content; biological activities of key proteins of the olfactory pathway and their corresponding compounds were verified by molecular docking technique; and finally pica in a rat model was established to verify the molecular mechanism of antiemetic effects of LEO by enzyme-linked immunosorbent assay (ELISA) to determine the serum 5-HT, substance P, and DA levels in each group and by immunohistochemistry to determine the contents of 5-HTR, CaMKII and ERK1/2 proteins in the medulla oblongata tissue.

Results: Network pharmacology combined with molecular docking analysis showed that the mechanism of the antiemetic effect of LEO may be related to (2Z)-3,7-dimethyl-2,6-octadienyl acetate, linalyl acetate, butanoic acid, hexyl ester, 4-hexen-1-ol, 5-methyl-2-(1-methylethenyl)-, acetate, .tau.-cadinol and other active ingredients, which regulate the cyclic adenosine monophosphate (cAMP) signaling pathway and the expression of BRAF, PDE and other targets on the pathway. An ELISA revealed that LEO reduced the levels of 5-hydroxytryptamine (5-HT), substance P, and dopamine in serum compared with the model group (P <0.05). Immunohistochemical analysis showed that LEO decreased the expression of 5-HTR, CaMKII, and ERK1/2 proteins in the medulla oblongata of rats compared with the model group (P <0.01).

Conclusion: LEO may achieve the antiemetic effect by reducing the content of 5-HT and inhibiting its related receptors, thereby regulating downstream Ca/CaMKII/ERK1/2 pathway of the cAMP signaling pathway.
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http://dx.doi.org/10.2147/DDDT.S366597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341382PMC
August 2022

Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike.

PLoS Pathog 2022 Jul 29;18(7):e1010583. Epub 2022 Jul 29.

Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.

The spike (S) protein of SARS-CoV-2 has been observed in three distinct pre-fusion conformations: locked, closed and open. Of these, the function of the locked conformation remains poorly understood. Here we engineered a SARS-CoV-2 S protein construct "S-R/x3" to arrest SARS-CoV-2 spikes in the locked conformation by a disulfide bond. Using this construct we determined high-resolution structures confirming that the x3 disulfide bond has the ability to stabilize the otherwise transient locked conformations. Structural analyses reveal that wild-type SARS-CoV-2 spike can adopt two distinct locked-1 and locked-2 conformations. For the D614G spike, based on which all variants of concern were evolved, only the locked-2 conformation was observed. Analysis of the structures suggests that rigidified domain D in the locked conformations interacts with the hinge to domain C and thereby restrains RBD movement. Structural change in domain D correlates with spike conformational change. We propose that the locked-1 and locked-2 conformations of S are present in the acidic high-lipid cellular compartments during virus assembly and egress. In this model, release of the virion into the neutral pH extracellular space would favour transition to the closed or open conformations. The dynamics of this transition can be altered by mutations that modulate domain D structure, as is the case for the D614G mutation, leading to changes in viral fitness. The S-R/x3 construct provides a tool for the further structural and functional characterization of the locked conformations of S, as well as how sequence changes might alter S assembly and regulation of receptor binding domain dynamics.
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http://dx.doi.org/10.1371/journal.ppat.1010583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365160PMC
July 2022

A Computationally Efficient and Virtualization-Free Two-Dimensional DOA Estimation Method for Nested Planar Array: RD-Root-MUSIC Algorithm.

Sensors (Basel) 2022 Jul 13;22(14). Epub 2022 Jul 13.

College of Electronic Information Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.

To address the problem of expensive computation in traditional two-dimensional (2D) direction of arrival (DOA) estimation, in this paper, we propose a 2D DOA estimation method based on a reduced dimension and root-finding MUSIC algorithm for nested planar arrays (NPAs). Specifically, the algorithm proposed in this paper transforms the problem based on 2D spectral peak search into two one-dimensional estimation problems by reducing the dimension, and then transforms the one-dimensional estimation problem into a problem of polynomial root finding. Finally the parameters are paired to realize the 2D DOA estimation. The proposed algorithm not only performs two root finding operations directly according to the 2D spectral function transformation, avoiding the performance degradation caused by intermediate operations, but can also fully exploit the enlarged array aperture offered by NPAs with reduced computational complexity and no need for virtualization. The superiorities of the proposed algorithm in terms of estimation accuracy and complexity are verified by simulations.
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http://dx.doi.org/10.3390/s22145220DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9325100PMC
July 2022

MARCKSL1-2 reverses docetaxel-resistance of lung adenocarcinoma cells by recruiting SUZ12 to suppress HDAC1 and elevate miR-200b.

Mol Cancer 2022 07 21;21(1):150. Epub 2022 Jul 21.

Department of Medical Oncology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, No.160 Pujian Road, Pudong New District, Shanghai, 200127, China.

Background: Long non-coding RNAs (lncRNAs) are implicated in the development of multiple cancers. In our previous study, we demonstrated that HDAC1/4-mediated silencing of microRNA-200b (miR-200b) enhances docetaxel (DTX)-resistance of human lung adenocarcinoma (LAD) cells.

Methods And Results: Herein, we probed the function of LncRNA MARCKSL1-2 (MARCKSL1-transcript variant 2, NR_052852.1) in DTX resistance of LAD cells. It was found that MARCKSL1-2 expression was markedly reduced in DTX-resistant LAD cells. Through gain- or loss- of function assays, colony formation assay, EdU assay, TUNEL assay, and flow cytometry analysis, we found that MARCKSL1-2 suppressed the growth and DTX resistance of both parental and DTX-resistant LAD cells. Moreover, we found that MARCKSL1-2 functioned in LAD through increasing miR-200b expression and repressing HDAC1. Mechanistically, MARCKSL1-2 recruited the suppressor of zeste 12 (SUZ12) to the promoter of histone deacetylase 1 (HDAC1) to strengthen histone H3 lysine 27 trimethylation (H3K27me3) of HDAC1 promoter, thereby reducing HDAC1 expression. MARCKSL1-2 up-regulated miR-200b by blocking the suppressive effect of HDAC1 on the histone acetylation modification at miR-200b promoter. Furthermore, in vivo analysis using mouse xenograft tumor model supported that overexpression of MARCKSL1-2 attenuated the DTX resistance in LAD tumors.

Conclusions: We confirmed that MARCKSL1-2 alleviated DTX resistance in LAD cells by abolishing the inhibitory effect of HDAC1 on miR-200b via the recruitment of SUZ12. MARCKSL1-2 could be a promising target to improve the chemotherapy of LAD.
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http://dx.doi.org/10.1186/s12943-022-01605-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9306054PMC
July 2022

The complete chloroplast genome sequence of (Asphodelaceae).

Mitochondrial DNA B Resour 2022 8;7(7):1227-1228. Epub 2022 Jul 8.

Institute of Floriculture, Liaoning Academy of Agricultural Sciences, Shenyang, China.

is a kind of wild plant with high ornamental value. In this study, we sequenced the complete chloroplast genome of by using Illumina sequencing techniques. The whole chloroplast genome was 156,063 bp in size, consisting of a large single-copy (LSC) region of 84,820 bp, a small single-copy (SSC) region of 18,505 bp, and a pair of inverted repeats (IRa and IRb) regions of 26,369 bp. The chloroplast genome contained 134 genes in total, including 88 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The overall GC content was 37.34%. Phylogenetic analysis showed that was closely related to of the same genus.
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http://dx.doi.org/10.1080/23802359.2022.2093663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275492PMC
July 2022

LAP+CD4+T cells regulate the anti-tumor role of CIK cells in colorectal cancer through IL-10 and TGF-β.

Am J Transl Res 2022 15;14(6):3716-3728. Epub 2022 Jun 15.

Department of Anorectal, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine Nanchang 330006, Jiangxi, China.

The rate of colorectal cancer (CRC) is increasing. Adoptive immune cell therapy (ACT) is a research hotspot in CRC treatment, and the common adoptive cells are cytokine-induced killer cells (CIK). The problem of ACT is that some regulatory T cells (Treg) will affect the efficacy. Latent associated polypeptide (LAP)+CD4+T is a new Treg, and its immunosuppressive effect is much higher than that of traditional Tregs. This research mainly explored the influence of LAP+CD4+T cells on anti-tumor lethality of CIK cells, so as to fill this gap. The LAP+CD4+T CIK cells and LAP-CD4+T CIK cells were sorted by immunomagnetic beads. LAP+CD4+T cells were expanded , and high expression cytokine genes were screened by RT-qPCR. LAP+CD4+T and LAP-CD4+T CIK cells were co-cultured to test cyto-activity. Transplanted tumor models of CRC were established in nude mice, which were randomized into a control group (CG), CIK group, LAP (-) group, LAP (+) group, IL-10 siRNA group, and TGF-siRNA group, and the tumor growth in each group was observed. The research results revealed that interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) were highly expressed in LAP+CD4+T cells. LAP+CD4+T could effectively suppress CIK cell proliferation and activity. LAP-CD4+T could suppress IL-10 and TGF-β, and inhibit CIK cell apoptosis, proliferation, and tumor growth, thus improving their anti-tumor lethality. LAP+CD4+T cells regulate the anti-tumor role of CIK cells in CRC through IL-10 and TGF-β.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274552PMC
June 2022

Analysis of levels and Clinical value of CA19-9, NLR and SIRI in patients with Pancreatic Cancer with different Clinical Features.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 4;67(5):302-308. Epub 2022 Feb 4.

Department of Hepatobiliary Surgery, Baoji Hospital of Traditional Chinese Medicine, Baoji, Shaanxi, China.

This study aimed to investigate the levels of cancer antigen 19-9 (CA19-9), neutrophil/lymphocyte ratio (NLR) and systemic immune-inflammatory index (SIRI) in patients with pancreatic cancer with different clinical features and their clinical value. For this purpose, 78 patients with pancreatic cancer treated in our hospital from January 2018 to January 2021 were divided into two groups according to the NLR and SIRI calculated by blood routine and biochemical examination before operation. To observe and analyze the relationship between serum CA19-19 levels, clinical features and NLR and SIRI values in patients with different clinical stages and tumor diameter, and the diagnostic value of CA19-9, NLR and SIRI in pancreatic cancer. The results showed that the level of serum CA19-9 in patients with stage IV pancreatic cancer was significantly higher than that in patients with stage I, II and III, and the level of CA19-9 in patients with stage III was higher than that in patients with stage I and II, and the level of CA19-9 in patients with stage II was higher than that in patients with stage I. The level of CA19-9 in patients with pancreatic cancer with tumor diameter ≥ 5cm was significantly higher than that in patients with tumor diameter < 5cm. The percentage of patients with age ≤ 60 years old in high NLR group (83.78%) was significantly higher than that in low NLR group (63.41%), and the rate of patients with tumor diameter ≥ 5cm in the high SIRI group (54.05%) was significantly higher than that in low NLR group (26.83%). The rate of patients with tumor diameter ≥ 5cm in the high SIRI group (61.92%) was significantly higher than that in the low SIRI group (31.58%). Among the single indexes, the sensitivity of CA19-9 in the diagnosis of pancreatic cancer was the highest (93.10%). The specificity of Siri in the diagnosis of pancreatic cancer was 89.70%. The sensitivity and specificity of combined detection were 89.7% and 70.00%, respectively. In general, the level of serum CA19-9 in patients with pancreatic cancer increases gradually with the increase of clinical stage and tumor diameter. NLR value is related to patient age and tumor diameter, and SIRI value is related to tumor diameter. Combined detection of CA19-9, NLR and SIRI are of higher value in the early diagnosis of pancreatic cancer.
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http://dx.doi.org/10.14715/cmb/2021.67.4.41DOI Listing
February 2022

Synthesis of Ag-Cu co-doping sponge iron-based trimetal for boosting simultaneous degradation of combined pollutants.

J Hazard Mater 2022 Jun 18;438:129413. Epub 2022 Jun 18.

Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment (MEE), Nanjing 210042, PR China; The Key Laboratory of Water and Air Pollution Control of Guangdong Province, South China Institute of Environmental Sciences, Ministry of Ecology and Environment (MEE), Guangzhou 510655, PR China. Electronic address:

To date, zero-valent iron (ZVI)-based technique has encountered a baffle, challenging simultaneous detoxification of refractory rhodamine B (RhB) and p-nitrophenol (PNP) possessing strong electronwithdrawing nitro-group. In this study, we synthesized Ag-Cu decorated sponge iron (s-Fe)-based trimetal for simultaneous degradation of RhB and PNP. The results show that Cu-Ag co-doping s-Fe (s-Fe-(Cu-Ag)) achieves approx. 90.6 % of maximized removal of RhB; the preferred s-Fe-(5 wt%Cu-1 wt%Ag) assisted with 6 L/min aeration rate simultaneously declines RhB and PNP within 10 recycling tests; non-aeration process obtains a complete reduction of PNP as well as merely approx. 23.9 % removal of RhB. Moreover, the Cu-Ag microstructure covering s-Fe-(Cu-Ag) has been characterized in detail. Furthermore, the electron spin resonance (ESR) spectra have been applied to investigate simultaneous generation of reactive oxygen species (ROS) and hydrogen radicals ([H]) over s-Fe-(Cu-Ag). To our best knowledge, this is the first study reporting the enhanced bifunctional catalysis of s-Fe-(Cu-Ag)/O for simultaneous degradation of RhB and PNP.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129413DOI Listing
June 2022

Understanding the Antecedents of the Routine Use of Mobile Health Services: A Person-Technology-Health Framework.

Front Psychol 2022 16;13:879760. Epub 2022 Jun 16.

Department of Logistics and Maritime Studies, Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.

Although numerous studies have been conducted to understand the antecedents of usage of mobile health (mHealth) services, most of them solely focus on characteristics of mHealth services themselves but neglect taking users' psychological and health-related factors into consideration. Besides, the comprehensive understanding of what influences users' routine use intentions regarding mHealth services is lacking. Therefore, this study proposes a person-technology-health framework that underlines how personal factors (e.g., personal innovativeness in IT), technological factors (e.g., trust), and health factors (e.g., perceived health severity) jointly influence individuals' routine use intentions regarding mHealth services. The proposed research model and related hypotheses were tested based on survey data from 270 respondents. The results indicate that personal innovativeness in IT, trust, and perceived health severity are important for enhancing routine use intention of mHealth services. Specifically, in situations of high perceived health severity, trust relates less positively to routine use intention than personal innovativeness in IT. In contrast, in situations of low perceived health severity, trust relates more positively to routine use intention than personal innovativeness in IT. The research findings extend the existing literature on routine use intention related to mHealth services and provide significant implications for practitioners.
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http://dx.doi.org/10.3389/fpsyg.2022.879760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245714PMC
June 2022

Distinguishable Prognostic Signatures and Tumor Immunogenicity Between Pancreatic Head Cancer and Pancreatic Body/Tail Cancer.

Front Oncol 2022 8;12:890715. Epub 2022 Jun 8.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Pancreatic head cancer and pancreatic body/tail cancer are considered to have different clinical presentations and to have altered outcomes.

Methods: Ninety cases of pancreatic adenocarcinoma (PDAC) from our institution were used as a discovery set and 166 cases of PDAC from the TCGA cohort were used as a validation set. According to the anatomical location, the cases of PDAC were divided into the pancreatic head cancer group and the pancreatic body/tail cancer group. Firstly, the different biological functions of the two groups were assessed by ssGSEA. Meanwhile, ESTIMATE and CIBERSORT were conducted to estimate immune infiltration. Then, a novel anatomical site-related risk score (SRS) model was constructed by LASSO and Cox regression. Survival and time-dependent ROC analysis was used to prove the predictive ability of our model in two cohorts. Subsequently, an integrated survival decision tree and a scoring nomogram were constructed to improve prognostic stratification and predictive accuracy for individual patients. In addition, gseaGO and gseaKEGG pathway analyses were performed on genes in the key module by the R package.

Results: Overall survival and the objective response rate (ORR) of patients with pancreatic body/tail cancer were markedly superior to those with pancreatic head cancer. In addition, distinct immune characteristics and gene patterns were observed between the two groups. Then, we screened 5 biomarkers related to the prognosis of pancreatic cancer and constructed a more powerful novel SRS model to predict prognosis.

Conclusions: Our research shed some light on the revelation of gene patterns, immune and mutational landscape characterizations, and their relationships in different PDAC locations.
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http://dx.doi.org/10.3389/fonc.2022.890715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213676PMC
June 2022

, Subfamily Genes of Transcription Factors From Cassava, Play an Important Role in Disease Resistance.

Front Plant Sci 2022 2;13:890555. Epub 2022 Jun 2.

Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, China.

Cassava ( Crantz) is an important tropical crop for food, fodder, and energy. Cassava bacterial blight (CBB) caused by pv. () occurs in all cassava growing regions and threatens global cassava production. WRKY transcription factor family plays the essential roles during plant growth, development, and abiotic or biotic stress. Particularly, previous studies have revealed the important role of the group IIa genes in plant disease resistance. However, a comprehensive analysis of group IIa subfamily in cassava is still missing. Here, we identified 102 members, which were classified into three groups, I, II, and III. Transient expression showed that six were localized in the nucleus. transcripts accumulated significantly in response to SA, JA, and . Overexpression of and in enhanced its resistance to DC3000. In contrast, silencing of and in cassava enhanced its susceptibility to . Co-expression network analysis showed that different downstream genes are regulated by different members. The functional analysis of downstream genes will provide clues for clarifying molecular mechanism of cassava disease resistance. Collectively, our results suggest that are regulated by SA, JA signaling, and coordinate response to infection.
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http://dx.doi.org/10.3389/fpls.2022.890555DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201764PMC
June 2022

H3K27ac mediated SS18/BAFs relocation regulates JUN induced pluripotent-somatic transition.

Cell Biosci 2022 Jun 16;12(1):89. Epub 2022 Jun 16.

CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy and Sciences, Guangzhou, 510530, China.

Background: The exit from pluripotency or pluripotent-somatic transition (PST) landmarks an event of early mammalian embryonic development, representing a model for cell fate transition.

Results: In this study, using a robust JUN-induced PST within 8 h as a model, we investigate the chromatin accessibility dynamics (CAD) as well as the behaviors of corresponding chromatin remodeling complex SS18/BAFs, to probe the key events at the early stage of PST. Here, we report that, JUN triggers the open of 34661 chromatin sites within 4 h, accomplished with the activation of somatic genes, such as Anxa1, Fosl1. ChIP-seq data reveal a rapid relocation of SS18/BAFs from pluripotent loci to AP-1 associated ones. Consistently, the knockdown of Brg1, core component of BAF complexes, leads to failure in chromatin opening but not closing, resulting in delay for JUN induced PST. Notably, the direct interaction between SS18/BAFs and JUN-centric protein complexes is undetectable by IP-MS. Instead, we show that H3K27ac deposited by cJUN dependent process regulates SS18/BAFs complex to AP1-containing loci and facilitate chromatin opening and gene activation.

Conclusions: These results reveal a rapid transfer of chromatin remodeling complexes BAF from pluripotent to somatic loci during PST, revealing a simple mechanistic aspect of cell fate control.
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http://dx.doi.org/10.1186/s13578-022-00827-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204951PMC
June 2022

Real-Valued Direct Position Determination of Quasi-Stationary Signals for Nested Arrays: Khatri-Rao Subspace and Unitary Transformation.

Sensors (Basel) 2022 May 31;22(11). Epub 2022 May 31.

College of Electronic and Information Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.

The features of quasi-stationary signals (QSS) are considered to be in a direct position determination (DPD) framework, and a real-valued DPD algorithm of QSS for nested arrays is proposed. By stacking the vectorization form of the signal's covariance for different frames and further eliminating noise, a new noise-eliminated received signal matrix is obtained first. Then, the combination of the Khatri-Rao subspace method and subspace data fusion method was performed to form the cost function. High complexity can be reduced by matrix reconstruction, including the modification of the dimension-reduced matrix and unitary transformation. Ultimately, the advantage of lower complexity, compared with the previous algorithm, is verified by complexity analysis, and the superiority over the existing algorithms, in terms of the maximum number of identifiable sources, estimation accuracy, and resolution, are corroborated by some simulation results.
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http://dx.doi.org/10.3390/s22114209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185511PMC
May 2022

Clinical Application and Evaluation of Metagenomic Next-Generation Sequencing in Pulmonary Infection with Pleural Effusion.

Infect Drug Resist 2022 1;15:2813-2824. Epub 2022 Jun 1.

Department of Respiratory and Critical Medicine, the Second Affiliated Hospital of Harbin Medical University, Heilongjiang, People's Republic of China.

Purpose: Metagenomic next-generation sequencing (mNGS) is a novel technique of pathogens detection that plays an increasingly important role in clinical practice. In this study, we explored the application value of mNGS in pulmonary infection combined with pleural effusion applied to samples of pleural effusion fluid.

Patients And Methods: We reviewed 80 cases of pulmonary infection with pleural effusion between August 2020 and October 2021. Among them, 40 patients were placed in the mNGS group and underwent both culture and mNGS testing; the patients in the control group were only subjected to culture test. The effectiveness of mNGS was evaluated for microbial composition and diagnostic accuracy in every pleural effusion specimen type.

Results: We found that the positive rate of mNGS was 70% (28/40). The comparison between mNGS and culture method resulted that the sensitivity was 100% (95% CI: 29.2-100%) and the specificity was 64.9% (95% CI: 47.5-79.8%). The positive predictive value of mNGS was 18.8% (95% CI, 13.0-26.3%), and the negative predictive value was 100%. The most commonly identified potential pathogens were bacteria, such as , , , and . The most detected fungal infection was and . A total of 11 patients were identified as mixed infection by mNGS. Treatment regimen adjustments were made according to mNGS results and the overall length of hospital stay in the mNGS group was shorter compared to that of the control group.

Conclusion: In this study, mNGS produced higher positive rates than the culture method in detecting pathogens in the pleural effusion specimens. The technology performed satisfactorily, providing more diagnostic evidence and reducing the length of hospital stay.
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http://dx.doi.org/10.2147/IDR.S365757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167844PMC
June 2022

Age and sex specific reference intervals of 13 hematological analytes in Chinese children and adolescents aged from 28 days up to 20 years: the PRINCE study.

Clin Chem Lab Med 2022 07 24;60(8):1250-1260. Epub 2022 May 24.

Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, P.R. China.

Objectives: Pediatric Reference Intervals in China (PRINCE) is a nationwide initiative that aims to establish and validate harmonized reference intervals (RIs) for Chinese children and adolescents, in which 15,150 healthy volunteers aged up to 20 years were recruited from 11 centers to establish RIs and 7,557 children and adolescents were enrolled from 21 centers to validate RIs.

Methods: The complete blood cell counts (CBC) of venous whole blood were measured by hematology analyzers through Sysmex systems in different centers. Age- and sex-specific RIs were calculated according to the guidelines.

Results: Unlike adults with certain levels of analyte concentrations, hematological parameters of children changed through growth and development. Red blood cell counts, hemoglobin, and hematocrit increased with age, and revealed higher concentrations in boys than girls after puberty. White blood cell counts and platelet counts showed significant higher levels than adults before 2 years of age, and then gradually decreased without distinct sex differences. In addition, lymphocyte counts decreased with age while neutrophil counts showed an opposite trend. The lower and upper limits of pediatric RIs of CBC were different from those of adults.

Conclusions: The validation of RIs indicated that the PRINCE study provided a version of RIs suitable for most of regions in China. This first harmonized pediatric RIs of CBC across China provided a robust database to understand the dynamic changes of hematologic parameters from birth to adolescence, and will contribute to clinical diagnosis and prognosis evaluation for pediatric patients as well.
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http://dx.doi.org/10.1515/cclm-2022-0304DOI Listing
July 2022

In-situ construction of step-scheme MoS/BiOBr heterojunction with improved photocatalytic activity of Rhodamine B degradation and disinfection.

J Colloid Interface Sci 2022 Oct 14;623:500-512. Epub 2022 May 14.

School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, PR China; Shaanxi University of Chinese Medicine/Shaanxi Collaborative Innovation Center of Idustrialization of Tradition Chinese Medicine Resources, Xianyang 712083, PR. China. Electronic address:

In this paper, a novel Step-scheme MoS/BiOBr heterojunction was fabricated through the in-situ mechanical agitation method and the photocatalytic activity of that was examined by the photocatalytic degradation Rhodamine B (RhB) and inactivation of E.coli under visible light irradiation (λ > 420 nm). The Step-scheme MoS/BiOBr heterojunctions displayed the enhanced photocatalytic ability compared to pure BiOBr and MoS and the MoS/BiOBr-3% (MS/BOB-3) heterojunction exhibited the strongest photocatalytic activity which can completely inactivate the 1 × 10 cfu/mL with 180 min and degrade RhB (10 mg/L) with 24 min visible light irradiation, respectively. The photocatalytic mechanism of the MoS/BiOBr heterojunction is was attributed to the generated active species (h, ·O and ·OH) which can effectively destroy RhB molecular and cell-membrane of bacterial as demonstrated by multiple techniques such as LC-MS and fluorescence stain. Additionally, characterization results disclosed that the transfer pathway of charge carriers of constructed MoS/BiOBr heterojunction followed the Step-scheme channel, which not only facilitated the separation and migration of the photo-generated charge carriers, but also improved the light absorption ability of the samples and resulting in the promoted photocatalytic performance of MoS/BiOBr heterojunction. This work paves a new idea to develop novel bismuth oxyhalide-based photocatalytic system for wastewater purification.
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http://dx.doi.org/10.1016/j.jcis.2022.04.148DOI Listing
October 2022

Cubic Nanogrids for Counterbalance Contradiction among Reorganization Energy, Strain Energy, and Wide Bandgap.

J Phys Chem Lett 2022 May 9;13(19):4297-4308. Epub 2022 May 9.

Center for Molecular Systems & Organic Devices (CMSOD), Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Molecular cross-scale gridization and polygridization of organic π-backbones make it possible to install 0/1/2/3-dimensional organic wide-bandgap semiconductors (OWBGSs) with potentially ZnO-like fascinating multifunctionality such as optoelectronic and piezoelectronic features. However, gridization effects are limited to uncover, because the establishment of gridochemistry still requires a long time, which offers a chance to understand the effects with a theoretical method, together with data statistics and machine learning. Herein, we demonstrate a state-of-the-art 3D cubic nanogridon with a size of ∼2 × 2 × 1.5 nm to examine its multigridization of π-segments on the bandgap, molecular strain energy (MSE), as well as reorganization energy (ROE). A cubic gridon (CG) consists of a four-armed bifluorene skeleton and a thiophene-containing fused arene plane with the Csp spiro-linkage, which can be deinstalled into face-on or edge-on monogrids. As a result, multigridization does not significantly reduce bandgaps ( ≥ 4.03 eV), while the MSE increases gradually from 4.72 to 23.83 kcal/mol. Very importantly, the ROE of a CG exhibits an extreme reduction down to ∼28 meV (λ) that is near the thermal fluctuation energy (∼26 meV). Our multigridization results break through the limitation of the basic positively proportional relationship between reorganization energies and bandgaps in organic semiconductors. Furthermore, multigridization makes it possible to keep the ROE small under the condition of a high MSE in OWBGS that will guide the cross-scale design of multifunctional OWBGSs with both inorganics' optoelectronic performance and organics' mechanical flexibility.
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http://dx.doi.org/10.1021/acs.jpclett.2c00827DOI Listing
May 2022

Analysis of 22-year Drought Characteristics in Heilongjiang Province Based on Temperature Vegetation Drought Index.

Comput Intell Neurosci 2022 28;2022:1003243. Epub 2022 Apr 28.

Institute of Agricultural Remote Sensing and Information, Heilongjiang Academy of Agricultural Sciences, Haerbin 150086, China.

Heilongjiang Province is the main grain producing region in China and an important part of Northeast China Plain, which is one of the three black soil belts in the world. The cultivated region of black soil accounts for 50.6% of the black soil region in Northeast China. Due to the obvious rise of temperature and uneven distribution of precipitation in the 20th century, it has been considered to be one of the important reasons for agricultural drought and aridity. Under the background of climate change, understanding the multiyear changes and occurrence characteristics of cultivated land drought in different agricultural regions in Heilongjiang Province is of great significance for the establishment of agricultural drought prediction and early warning system in the future, guiding agricultural high-standard farmland irrigation in different regions, promoting black soil protection, and then improving grain yield. This paper calculates the temperature vegetation drought index (TVDI) based on the normalized difference vegetation index (NDVI) and surface temperature (TS) product data of MODIS from 2000 to 2021. Taking TVDI as the drought evaluation index, this paper studies the temporal and spatial variation distribution characteristics and occurrence frequency of drought in the whole region and four agricultural regions of Heilongjiang Province: Daxing an Mountain and Xiaoxing an Mountain (region I), Sanjiang Plain (region II), Zhangguangcai Mountains (region III), and Songnen Plain (region IV). The results show that medium drought generally occurred in Heilongjiang Province from 2000 to 2021, accounting for about 70% of the total cultivated land. The drought was severe from 2000 to 2009 and weakened from 2010 to 2021. In the 110 months of the crop growing season from 2000 to 2021, about 63.84% of the region suffered more than 60 droughts. It is found that the frequency of drought varies from region to region. More than 80 droughts occurred in the west of region IV and the middle of region II. The characteristics of region IV are large sandstorm, less precipitation, and lack of water conservancy facilities, resulting in frequent and strong drought. It is also found that the occurrence frequency, degree grade and regional distribution of drought are closely related to seasonal changes. In spring, the occurrence grade and frequency of drought in region IV are the strongest and the drought phenomenon is serious. In autumn, drought is frequent and distributed in all regions, but the grade is not strong (mainly medium drought), and the drought phenomenon is medium. It is humid in summer. Crops in Heilongjiang Province are one crop per annual. Spring drought seriously restricts the water content of crops. Long-term drought will lead to poor crop development and reduce yield. Therefore, only by clarifying the characteristics of regional time drought, monitoring accurate drought events and accurately predicting the occurrence of drought, can we guide high-standard farmland precision irrigation, improve crop yield and ensure national food security. At the same time, severe drought will affect the terrestrial ecosystem, resulting in the distribution of crops and microorganisms, and the transformation between carbon sink and carbon source.
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http://dx.doi.org/10.1155/2022/1003243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071970PMC
May 2022

A novel monoacylglycerol lipase-targeted F-labeled probe for positron emission tomography imaging of brown adipose tissue in the energy network.

Acta Pharmacol Sin 2022 May 5. Epub 2022 May 5.

Division of Nuclear Medicine and Molecular Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Monoacylglycerol lipase (MAGL) constitutes a serine hydrolase that orchestrates endocannabinoid homeostasis and exerts its function by catalyzing the degradation of 2-arachidonoylglycerol (2-AG) to arachidonic acid (AA). As such, selective inhibition of MAGL represents a potential therapeutic and diagnostic approach to various pathologies including neurodegenerative disorders, metabolic diseases and cancers. Based on a unique 4-piperidinyl azetidine diamide scaffold, we developed a reversible and peripheral-specific radiofluorinated MAGL PET ligand [F]FEPAD. Pharmacokinetics and binding studies on [F]FEPAD revealed its outstanding specificity and selectivity towards MAGL in brown adipose tissue (BAT) - a tissue that is known to be metabolically active. We employed [F]FEPAD in PET studies to assess the abundancy of MAGL in BAT deposits of mice and found a remarkable degree of specific tracer binding in the BAT, which was confirmed by post-mortem tissue analysis. Given the negative regulation of endocannabinoids on the metabolic BAT activity, our study supports the concept that dysregulation of MAGL is likely linked to metabolic disorders. Further, we now provide a suitable imaging tool that allows non-invasive assessment of MAGL in BAT deposits, thereby paving the way for detailed mechanistic studies on the role of BAT in endocannabinoid system (ECS)-related pathologies.
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http://dx.doi.org/10.1038/s41401-022-00912-8DOI Listing
May 2022

Effect of immune checkpoint inhibitors in patients with gastric hepatoid adenocarcinoma: a case report and literature review.

J Int Med Res 2022 Apr;50(4):3000605221091095

Department of Oncology, Huaian Hospital of Huaian City, No. 161 Zhenhuailou East Road, Huaian 223200, Jiangsu Province, People's Republic of China.

Gastric hepatoid adenocarcinoma (GHAC) is a highly aggressive histological subtype of gastric cancer (GC) with similar tissue morphology to hepatocellular carcinoma. GHAC frequently produces alpha-fetoprotein (AFP) and has a poor prognosis; however, standardized treatment remains elusive. We report a male patient in his early 60s with GHAC who received immunotherapy, and the curative effect was evaluated. He was admitted because of progressive fatigue and dizziness for 2 months. He had experienced spontaneous epigastric pain with muscular defense of the epigastrium and accompanying tenderness 1 year earlier and underwent radical gastrectomy. Immunohistochemistry showed that hepatocyte-specific marker (Hep) was highly-expressed, indicating probable GHAC. Additionally, imaging suggested GC recurrence or gastric stump cancer. Radioimmunoassay indicated an AFP level of >1210.00 µg/L, and liver biopsy was performed following abdominal contrast-enhanced computed tomography. Pathology showed a few hepatocytes and proliferative fibrous connective tissue. The patient received three cycles of chemotherapy, with no obvious improvement. The possibility of surgical treatment was excluded, and immunotherapy or palliative treatment was selected. He received 11 cycles of a programed death-1 (PD-1) monoclonal antibody, and the effect of treatment was satisfactory. The mechanism of action of immunotherapy in GHAC warrants further investigation.
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http://dx.doi.org/10.1177/03000605221091095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087251PMC
April 2022

Synergistic blocking of RAS downstream signaling and epigenetic pathway in mutant pancreatic cancer.

Aging (Albany NY) 2022 04 25;14(8):3597-3606. Epub 2022 Apr 25.

State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal malignancy and lacks effective therapeutic targets. Trametinib is considered to be a promising potential indirectly targeted KRAS inhibitor in PDAC. However, the clinical outcomes were poor. JQ1 displayed a significant synergistic effect when combined with chemotherapy or potential targeted therapy in pancreatic cancer. The impact of Trametinib and JQ1 combination treatment in PDAC remains to be fully elucidated.

Methods: The efficacy of trametinib and JQ1 on cell proliferation and cytotoxicity was assayed in 7 mutant pancreatic cancer cell lines. The cytotoxic effects of drugs either alone or in combination were evaluated using a luminescent cell viability assay. Immunoblot analysis was carried out to investigate changes in p62 and autophagy.

Results: We found that either trametinib or JQ1 alone inhibited the proliferation of some pancreatic cancer cell lines with alterations, irrespective of the mutational loci of and the aberrant status of the other driver genes. The synergistic effects of combination treatment of trametinib and JQ1 were observed in both trametinib-resistant and trametinib-sensitive cells. In trametinib-sensitive PDAC cells, the combined treatment definitely inhibited p62 expression compared with trametinib alone, while LC3 expression at high levels changed little. In trametinib-resistant PDAC cells, the combination of MEK/BET inhibitor dramatically decreased p62 expression compared with single agent, while p62 expression increased after anti-autophagic therapy was added.

Conclusions: Blocking RAS downstream signaling and epigenetic pathway synergistically increases the antiproliferative activity in mutant PDAC cells. Combination therapeutic synergism may induce different cell death modes in different pancreatic cancer subtypes.
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http://dx.doi.org/10.18632/aging.204031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085242PMC
April 2022

Sensor-guided gap balance versus manual gap balance in primary total knee arthroplasty: a meta-analysis.

J Orthop Surg Res 2022 Apr 19;17(1):243. Epub 2022 Apr 19.

Department of Orthopedic, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, No. 168 Litang Road, Dongxiaokou Town, Changping District, Beijing, 102218, China.

Background: Despite Vast improvements in technology and surgical technique in total knee arthroplasty (TKA), approximately 15-25% TKAs, have suboptimal subjective clinical outcomes. Our study sought to evaluate if sensor-guided balancing improves postoperative clinical outcomes compared to a conventional gap balancing technique.

Methods: We searched Web of Science, Embase, PubMed, Cochrane Controlled Trials Register, Cochrane Library, Highwire, CBM, CNKI, VIP, and Wanfang database in March 2022 to identify studies involving sensor-guided balancing versus conventional gap balancing technique in TKA. Finally, we identified 2147 knees assessed in nine studies.

Results: Compared with manual gap balancing, Sensor-guided gap balancing resulted in less rate of Manipulation under anesthesia (MUA) (P = 0.02), however more rate of intraoperative additional procedures (P = 0.0003). There were no significant differences in terms of KSS (P = 0.21), KSS Function score (P = 0.36), OKS (P = 0.61), KOOS (P = 0.78), operative time (P = 0.17), Mechanical axis (P = 0.69) and rate of reoperation between two groups.

Conclusion: Compared with conventional manual gap balancing techniques, sensors have more balancing procedures being performed. However, it did result in a reduction in the rate of MUA. More extensive, high-quality RCTs are required to verify our findings further.
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http://dx.doi.org/10.1186/s13018-022-03129-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9020069PMC
April 2022

Regioselective Pd-catalyzed α-alkylation of furans using alkyl iodides.

RSC Adv 2021 Apr 14;11(23):13832-13838. Epub 2021 Apr 14.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences 555 Zuchongzhi Road Shanghai 201203 China.

Herein, direct alkylation of the C-H bond at the α-position of furans catalyzed by palladium catalyst is reported. This protocol targets α-alkylfurans, achieving moderate to good yields under very practical reaction conditions. With a broad scope of substrates and good functional group tolerance, this method will has promising utility in medicinal chemistry.
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http://dx.doi.org/10.1039/d1ra01522bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697702PMC
April 2021

TiO with Confined Water Boosts Ultrahigh Selective Enrichment of Phosphorylated Proteins.

ACS Appl Mater Interfaces 2022 Apr 14;14(16):19067-19075. Epub 2022 Apr 14.

CAS Key Laboratory of Bio-inspired Materials and Interface Sciences, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.

In the selective enrichment of phosphorylated proteins (PPs) from biological samples, the non-phosphorylated proteins (NPPs) adhered onto enrichment adsorbents due to the hydrophobic interaction, resulting in poor selectivity and low recovery of target PPs. Herein, superhydrophilic TiO-coated porous SiO microspheres are prepared and boost remarkable selectivity toward standard PP spiked with 2000 mass-fold NPP interference. The outstanding performance of the superhydrophilic microspheres is attributed to the coordination interaction between TiO and PPs, and the confined water layer generated from superhydrophilicity avoids the irreversible adsorption of NPPs by keeping NPP inner hydrophobic regions in a compact structure, which is verified by single molecule force spectroscopy, circular dichroism, and quartz crystal microbalance. This strategy for enrichment is expected to solve the challenge in proteomics and sheds light on the interactions between biomolecules and superwettability.
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http://dx.doi.org/10.1021/acsami.2c03158DOI Listing
April 2022

Layer-by-Layer Assembly of [email protected] Nanocomposites and CNTs as a Multifunctional Separator Coating for Highly Stable Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2022 Apr 12;14(16):18634-18645. Epub 2022 Apr 12.

Institute of Industry & Equipment Technology, School of Materials Science and Engineering, Key Laboratory of Advanced Functional Materials Devices of Anhui Province, Engineering Research Center of Advanced Composite Materials Design; Application of Anhui Province, Hefei University of Technology, Hefei 230009, China.

Commercialization of high-energy Li-S batteries is greatly restricted by their unsatisfactory cycle retention and poor cycling life originated from the notorious "shuttling effect" of lithium polysulfides. Modification of a commercial separator with a functional coating layer is a facile and efficient strategy beyond nanostructured composite cathodes for suppressing polysulfide shuttling. Herein, a multilayered functional [email protected]/CNT separator was successfully achieved by alternately depositing conductive carbon nanotubes (CNTs) and synthetic [email protected] onto the surface of the commercial separator. The cooperation of multiple components including Ce-MOF-derived [email protected], rGO, and CNTs enables the as-built [email protected]/CNT separator to perform multifunctions from the separator surface: (i) to hinder the diffusion of polysulfide species through physical blocking or chemical adsorption, (ii) to accelerate the sluggish redox reactions of sulfur species, and (iii) to enhance the conductivity for sulfur re-activation and efficient utilization. Serving as a multilayer and powerful barrier, the [email protected]/CNT separator greatly constrains and reutilizes the polysulfide species. Thus, the Li-S battery assembled with the [email protected]/CNT separator demonstrates an excellent combination of capacity, rate capability, and cycling performances (an initial capacity of 1107 mA h g with a low decay rate of 0.060% per cycle over 500 cycles at 1 C, 651 mA h g at 5 C) together with remarkably mitigated self-discharge and anode corrosion. This work provides guidelines for functional separator design as well as rare-earth material applications for Li-S batteries and other energy storage systems.
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http://dx.doi.org/10.1021/acsami.2c03461DOI Listing
April 2022

Design, synthesis, and biological evaluations of substituted pyrazoles as pyrrolomycin analogues against staphylococcal biofilm.

Eur J Med Chem 2022 Jun 1;236:114309. Epub 2022 Apr 1.

School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Jiangsu, 210046, China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai, 201203, China; School of Pharmacy, University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China. Electronic address:

The formation of biofilm enables Staphylococcus aureus to resist antibiotics and causes chronic infections. Several compounds of pyrrolomycins are potent antibacterial agents which display inhibition upon staphylococcal biofilms. We designed and synthesized two series of substituted pyrazoles as pyrrolomycin analogues. Compounds 17a, 17d and 17h displayed potent antibacterial activity against various vancomycin-resistant Enterococcus faecalis (VRE) and methicillin-resistant Staphylococcus aureus (MRSA), and 17d showed the most potent activity against MRSA (MIC = 0.0625 μg/mL), vancomycin-intermediate Staphylococcus aureus (VISA) (MIC = 0.0313 μg/mL). Further study indicated that compound 17h could significantly reduce the biofilm formation of MRSA and exhibited promising selectivity. In vitro liver microsomal stability was also evaluated and the results manifested that 17h was metabolically stable in human liver microsomes.
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http://dx.doi.org/10.1016/j.ejmech.2022.114309DOI Listing
June 2022

Association of Body Mass Index and Abdominal Obesity with the Risk of Airflow Obstruction: National Health and Nutrition Examination Survey (NHANES) 2007-2012.

COPD 2022 ;19(1):99-108

Department of Epidemiology and Health Statistics, The School of Public Health, Qingdao University, Qingdao, Shandong, China.

This study aimed to explore the relationship between body mass index (BMI) and abdominal obesity and the risk of airflow obstruction, based on the data from the 2007-2012 National Health and Nutrition Survey (NHANES). Logistic regression was applied to assess the relationships between BMI or abdominal obesity and the risk of airflow obstruction by the fixed ratio method and the lower limit of normal (LLN) method. We further used the restricted cubic splines with 3 knots located at the 5th, 50th, and 95th percentiles of the distribution to evaluate the dose-response relationship. A total of 12,865 individuals aged 20-80 years old were included. In the fixed ratio method, underweight was positively correlated with the risk of airflow obstruction, and overweight and obesity were negatively correlated with the risk of airflow obstruction. In the LLN method, the results were consistent with the fixed ratio method. Abdominal obesity was positively associated with the risk of airflow obstruction only in the fixed ratio method (OR: 1.41, 95% CI: 1.04-1.90). There was an additive interaction between underweight and smoking on airflow obstruction in both methods. Abdominal obesity and smoking had additive interactions in the LLN method. Dose-response analysis indicated that there was a non-linear trend between BMI and the risk of airflow obstruction ( < 0.01). Our study suggested that underweight and abdominal obesity were associated with the increased risk of airflow obstruction, and overweight and general obesity were associated with the decreased risk of airflow obstruction.
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http://dx.doi.org/10.1080/15412555.2022.2032627DOI Listing
April 2022

Severe Cholestatic Hepatitis Secondary to SARS-CoV-2.

ACG Case Rep J 2022 Mar 28;9(3):e00753. Epub 2022 Mar 28.

Division of Gastroenterology and Hepatology, University of Wisconsin-Madison, Madison, WA.

Liver injury is a common manifestation of coronavirus disease 2019 (COVID-19), with most injuries manifesting as transient mild hepatocellular injury. Cholestatic injury occurs less commonly and is typically mild. Severe cholestatic injury is rare, with only 4 cases reported in the literature. We present a 70-year-old woman with no known liver disease who presented with severe COVID-19 and developed severe cholestatic hepatitis. A liver biopsy was performed demonstrating bile duct injury, uncommonly reported in patients with COVID-19. This complication needs greater awareness because it has been known to cause progressive liver disease requiring transplantation.
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http://dx.doi.org/10.14309/crj.0000000000000753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963841PMC
March 2022
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