Publications by authors named "Xiaofei Sun"

160 Publications

Investigating phosphorescence capability of halogen-substituted metal-free organic molecules: A theoretical study.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Mar 9;255:119642. Epub 2021 Mar 9.

International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Institute of Theoretical Chemistry, College of Chemistry, Jilin University, Changchun 130023, China. Electronic address:

The radiative and non-radiative decay processes of five compounds are investigated through a comprehensive computational approach, for the aim of investigating the effect of different halogen substituents to the phosphorescent emission. Their optimal configurations at the ground (S) and lowest triplet excited (T) states are obtained and the calculated phosphorescent emission spectra are comparable with the experimental values. For 1,4-di(9H-carbazol-9-yl)benzene (PDCz), the electronic transition is between the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO), while for the four halides, the electronic transitions are attributed to several molecular orbitals. According to calculations, 9,9'-(2,5-diiodo-1,4-phenylene)bis(9H-carbazole) (PDICz) possesses the largest radiative decay rate constant (k) and non-radiative decay rate constant (k), which can be attributed to the strong spin-orbital coupling from the heavy iodine atom. However, the phosphorescent quantum efficiency (Φ) of PDICz is lower than that of 9,9'-(2,5-dibromo-1,4-phenylene)bis(9H-carbazole) (PDBCz), implying that a comprehensive consideration is necessary. Furthermore, by analyzing the vibrational mode, we have confirmed that the reorganization energies are also influenced by the different halogen atoms. While the dominated factor that determines the k and k comes from the spin-orbital coupling. We expect that our research findings will be beneficial to the newly designed organic phosphorescent materials in the future.
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http://dx.doi.org/10.1016/j.saa.2021.119642DOI Listing
March 2021

Influence of Methylenetetrahydrofolate Reductase C677T and A1298C Polymorphism on High-Dose Methotrexate-Related Toxicities in Pediatric Non-Hodgkin Lymphoma Patients.

Front Oncol 2021 26;11:598226. Epub 2021 Feb 26.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: This retrospective study aimed to investigate the relationships between the methylenetetrahydrofolate reductase (MTHFR) C677T/A1298C and high-dose methotrexate (HD-MTX)-related toxicities in pediatric non-Hodgkin lymphoma (NHL) patients.

Patients And Methods: We reviewed the medical records of 93 NHL patients aged under 18 years who received HD-MTX therapy at the dose of 5 g/m with 24-h infusion at Sun Yat-sen University Cancer Center between 2014 and 2019.

Results: There were 61 males and 32 females, with a median age of 8.8 years (0.9-15.8 years). The tumor types included lymphoblastic lymphoma (n = 38), Burkitt's lymphoma (n = 31), anaplastic large cell lymphoma (n = 18), diffuse large B-cell lymphoma (n = 6). Overall, 355 courses of HD-MTX therapy were prescribed. All patients were rescued with calcium folinate 12 h after the end of MTX infusion. We found that plasma MTX levels > 0.2 μmol/L at 48 h post-infusion increased the risk of developing oral mucositis (2.4% VS. 9.5%, P = 0.018). Also, patients carrying the C677T and T677T genotypes had tendencies to be more susceptible to oral mucositis (P = 0.034). Patients harboring mutant 677T allele were more likely to develop leucopenia (38.5 vs. 50.3%, P = 0.025) and thrombocytopenia (22.0 vs. 32.4%, P = 0.028). For polymorphism A1298C, the mutant genotype played a protective role in vomiting (11.1 vs. 4.3%, P = 0.018) but increased the risk of anemia (23.8 vs. 41.7%, P < 0.001) and leucopenia (38.1 vs. 50.3%, P = 0.021).

Conclusion: Childhood NHL patients harboring C677T genotype were more vulnerable to oral mucositis, leucopenia, and thrombocytopenia, while those with A1298C genotype were at a decreased risk of vomiting and more likely to develop anemia and leucopenia.
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http://dx.doi.org/10.3389/fonc.2021.598226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953141PMC
February 2021

Comparison of Surgical Results of the Bridge Crane Technique Versus Laminectomy for the Treatment of Thoracic Myelopathy Caused by Ossification of the Ligamentum Flavum.

Global Spine J 2021 Mar 9:2192568221996689. Epub 2021 Mar 9.

Department of Spine Surgery, Changzheng Hospital, Naval Medical University, Shanghai, People's Republic of China.

Study Design: A retrospective study.

Objectives: This study aimed to evaluate the safety and effectiveness of the bridge crane technique versus laminectomy for the treatment of thoracic myelopathy caused by ossification of the ligamentum flavum (OLF).

Methods: Totally 41 patients who underwent surgical decompression due to thoracic OLF from May 2017 to June 2018 in our institution were enrolled in this study and were divided into group BG (bridge crane technique, n = 19) and group L (laminoectomy, n = 22). Demographic data was collected from medical records and the modified Japanese Orthopaedic Association (JOA) scoring system was used to evaluate the neurological outcomes during the follow-up. Surgery-related complications were analyzed.

Results: The mean duration of follow-up was comparable between group BG (19.4 ± 1.5 months) and group L (19.6 ± 1.4 months). No statistical differences were observed between two groups in terms of gender, age, duration of symptoms, preoperative occupying rate, involved levels, operation time, intraoperative blood loss, and complications. The JOA score significantly increased at the final follow-up in both groups. However, patients in group BG had higher JOA score and recovery rate ( < 0.05). Four patients in group L experienced complications, including 3 cerebrospinal fluid (CSF) leakage and one postoperative hematoma. Only one patient in group BG had CSF leakage.

Conclusion: The results of this study suggested that bridge crane technique may be relatively safe and effective for patients with symptomatic thoracic OLF with more satisfactory clinical improvement. However, high-quality studies are still required to validate the results of this study.
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http://dx.doi.org/10.1177/2192568221996689DOI Listing
March 2021

Identification of prognostic immune-related genes in rhabdoid tumor of kidney based on TARGET database analysis.

Aging (Albany NY) 2021 02 11;13(4):5461-5474. Epub 2021 Feb 11.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong 510060, P.R. China.

Background: Malignant rhabdoid tumor of the kidney (RTK) is a rare and highly aggressive pediatric malignancy. Immune system dysfunction is significantly correlated with tumor initiation and progression.

Methods: We integrated and analyzed the expression profiles of immune-related genes (IRGs) in 65 RTK patients based on the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Prognostic related IRGs in RTK patients were analyzed using univariate and multivariate analysis, based on which a prognostic model with IRGs was constructed. Correlation analysis between the risk score of our model and tumor-infiltrating cell were also investigated.

Results: Twenty two IRGs were significantly associated with the clinical outcomes of RTK patients. Gene ontology (GO) analysis revealed that inflammatory pathways were most frequently implicated in RTK. A prognostic model was constructed using 7 IRGs (), which were independent prognostic indices that could differentiate patients based on their survival outcomes. Furthermore, the risk scores from our prognostic model was positively associated with cancer-associated fibroblasts (CAFs).

Conclusions: We screened seven IRGs of clinical significance to distinguish patients with different survival outcomes. This may enhance our understanding of the immune microenvironment of RTK, and could use to design individualized treatments for RTK patients.

Background: Malignant rhabdoid tumor of the kidney (RTK) is a rare and highly aggressive pediatric malignancy. Immune system dysfunction is significantly correlated with tumor initiation and progression.

Methods: We integrated and analyzed the expression profiles of immune-related genes (IRGs) in 65 RTK patients based on the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. Prognostic related IRGs in RTK patients were analyzed using univariate and multivariate analysis, based on which a prognostic model with IRGs was constructed. Correlation analysis between the risk score of our model and tumor-infiltrating cell were also investigated.

Results: Twenty two IRGs were significantly associated with the clinical outcomes of RTK patients. Gene ontology (GO) analysis revealed that inflammatory pathways were most frequently implicated in RTK. A prognostic model was constructed using 7 IRGs (), which were independent prognostic indices that could differentiate patients based on their survival outcomes. Furthermore, the risk scores from our prognostic model was positively associated with cancer-associated fibroblasts (CAFs).

Conclusions: We screened seven IRGs of clinical significance to distinguish patients with different survival outcomes. This may enhance our understanding of the immune microenvironment of RTK and could use to design individualized treatments for RTK patients.
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http://dx.doi.org/10.18632/aging.202475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950296PMC
February 2021

Zinc and Cadmium Complexes of Chelating N-Heterocyclic Silylene and Their Reactivity toward Elemental Chalcogens.

Inorg Chem 2021 Feb 15. Epub 2021 Feb 15.

Institut für Anorganische Chemie, Karlsruher Institut für Technologie, Engesserstraße 15, 76131 Karlsruhe, Germany.

The reactions of the pyridine-functionalized silylene LSi [L = PhC(NBu); N = 2-(methylamido)pyridine] with zinc and cadmium halides are described. These resulted in the formation of a series of zinc and cadmium silylene complexes: [LSi-ZnX] (X = Cl, Br, I) and [{LSi-CdI}], which is the first cadmium silylene compound. Subsequent reaction of these silylene complexes with elemental sulfur and selenium under mild conditions at room temperature afforded under activation of these elements the corresponding silanethione-stabilized zinc [(LSi═S)-ZnX] (X = Cl, I) and cadmium [(LSi═S)-CdI] complexes and the silaneselenone-stabilized zinc species [(LSi═Se)-ZnCl]. A selective insertion of the group 16 elements into the M-Si bond of the silylenes was observed. Because of metal coordination, the Si-chalcogen bond lengths in the silanethiones and silaneselenones are enlarged and thus range between a single and a double bond. All new compounds were fully characterized by single-crystal X-ray diffraction analyses, multinuclear NMR spectroscopy, elemental analyses, and IR spectroscopy.
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http://dx.doi.org/10.1021/acs.inorgchem.0c03609DOI Listing
February 2021

Natural alumina/silica suspended particles in water to enhance ofloxacin degradation with UVA-HO driven by surface chemistry.

J Hazard Mater 2021 Jan 29;412:125259. Epub 2021 Jan 29.

Faculty of Materials and Chemistry, China University of Geosciences, Wuhan 430074, PR China.

UV-HO is the most widely used oxidizing system with established effectiveness and a high level of technical development for practical application. However, little attention was paid on the effect of suspended particles in natural water on organic contaminants removal via UV-HO technique. In this study, this effect of suspended particles to enhance the contaminant degradation was explored using silica/alumina-based oxides (MCM-41 and Al@MCM-41) as the representative. The results showed that MCM-41 had no effect on OFX degradation compared with UV-HO. While the degradation efficiency and reaction rate were greatly enhanced at a pH range of 3.0-9.0 especially at acidic pH values (3.0-5.0) in the presence of Al@MCM-41. The probe experiments proved that OFX adsorption followed by surface reaction process played an important role to enhance the performance of UV-HO. Based on the characterization results, the positive effect of suspended particles was not related to their surface area and pore size distribution, but dependent on the chemical composition and surface acid-base property. The suspended particles can provide an active surface composed of acid and base sites. The base site can create a local basic micro-environment by producing more OH et al. While the dissociated acid sites in Al@MCM-41 with a negative charged surface favor OFX adsorption and then reaction with produced ROS. Our findings suggest that the enhanced performance of UVA-HO induced by suspended particles should be concerned.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125259DOI Listing
January 2021

Multimodal Treatment of Children With Sacrococcygeal Yolk Sac Tumor: Retrospective Analysis of Clinicopathology Characteristics and Relapse-free Survival.

J Pediatr Hematol Oncol 2021 Jan 27. Epub 2021 Jan 27.

Department of Pediatric Oncology, Sun Yat-sen University Cancer Center Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Department of Pediatric Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Zhongshan Er Lu, Guangzhou, China.

The aim of the study was to explore the clinicopathologic characteristics of sacrococcygeal yolk sac tumor (SYST) associated with relapse and the role of sensitivity to neoadjuvant chemotherapy in predicting outcome. The authors investigated prognostic factors of age, stage, initial tumor size, pathologic response to neoadjuvant chemotherapy, and alfa fetoprotein. A total of 26 patients with SYST were enrolled. Neoadjuvant chemotherapy was administered to 20 cases. Six patients underwent resection as initial therapy. Recurrence occurred in 12 patients. Nine patients with specimens exhibiting no malignant component after chemotherapy did not experience recurrence. By contrast, relapses occurred in 7 out of 11 patients with viable residual tumor after neoadjuvant chemotherapy. All relapsed patients still achieved partial remission or complete remission after salvage therapy. Five-year relapse-free survival and overall survival rates were 55.2% and 100%, respectively (median follow-up, 59.5 mo; range, 16 to 155). Patients with complete necrosis after neoadjuvant chemotherapy had a better outcome compared with children with viable residual tumor. Relapse-free survival of pediatric SYSTs in this cohort were still low and warrants the multidisciplinary effort.
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http://dx.doi.org/10.1097/MPH.0000000000002068DOI Listing
January 2021

Comparison of chemotherapy with or without asparaginase for extranodal nasal NK/T-cell lymphoma in children and adolescents.

Pediatr Blood Cancer 2021 May 23;68(5):e28901. Epub 2021 Jan 23.

Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: As extranodal natural killer/T-cell lymphoma (ENKTL) occurs rarely in children and adolescents, standardized therapy is yet to be determined. This study aimed to describe the clinical features and determine the optimal chemotherapy regimen for childhood ENKTL.

Methods: The treatment outcomes of radiotherapy combined with asparaginase-based (P-GEMOX or P-GMED) or asparaginase-absent chemotherapy regimens (CHOP, EPOCH, or NHL-BFM-90/95) in patients aged ≤18 years with newly diagnosed ENKTL from December 2006 to December 2018 were compared.

Results: Among the 34 patients included in the study, 21 had stage I/II disease. The overall response rates of chemotherapy with or without asparaginase were 85.0% and 78.6%, respectively. With a median follow-up of 54 months, the 5-year event-free survival (EFS) rates of patients with stage I/II and III/IV disease were 66.2 ± 11.3% and 26.0 ± 12.8%, respectively (P = .027). In stage III/IV patients treated with asparaginase-based or asparaginase-absent regimens, the 5-year EFS rates were 40.0 ± 17.4% and 0%, respectively (P = .236). The 5-year EFS rates of stage III/IV patients who received or did not receive hematopoietic stem cell transplant were 66.7 ± 27.2% and 11.1 ± 10.5%, respectively (P = .054). In addition, chemotherapy-associated side effects were significantly less in patients treated with asparaginase-based regimens as compared to asparaginase-absent regimens in this cohort.

Conclusion: P-GEMOX and P-GMED regimens are effective and safe for treating childhood ENKTL.
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http://dx.doi.org/10.1002/pbc.28901DOI Listing
May 2021

Imaging and Analysis of Isomeric Unsaturated Lipids through Online Photochemical Derivatization of Carbon-Carbon Double Bonds*.

Angew Chem Int Ed Engl 2021 03 26;60(14):7559-7563. Epub 2021 Feb 26.

Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.

Unraveling the complexity of the lipidome requires the development of novel approaches for the structural characterization of lipid species with isomer-level discrimination. Herein, we introduce an online photochemical approach for lipid isomer identification through selective derivatization of double bonds by reaction with singlet oxygen. Lipid hydroperoxide products are generated promptly after laser irradiation. Fragmentation of these species in a mass spectrometer produces diagnostic fragments revealing the C=C locations in the unreacted lipids. This approach uses an inexpensive light source and photosensitizer making it easy to incorporate into any lipidomics workflow. We demonstrate the utility of this approach for the shotgun profiling of C=C locations in different lipid classes present in tissue extracts using electrospray ionization (ESI) and ambient imaging of lipid species differing only by the location of C=C bonds using nanospray desorption electrospray ionization (nano-DESI).
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http://dx.doi.org/10.1002/anie.202016734DOI Listing
March 2021

[Knockdown of long non-coding RNA actin filament-associated protein 1 antisense RNA1 (AFAP1-AS1) inhibits epithelial-mesenchymal transition, invasion and migration of TPC-1 papillary thyroid cancer cells].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2021 Jan;37(1):54-60

Department of Endocrinology and Metabolism, Zhengzhou People's Hospital, Zhengzhou 450000, China.

Objective To detect the expression of long non-coding RNA (lncRNA) actin filament-related protein 1 antisense RNA1 (AFAP1-AS1) in papillary thyroid carcinoma tissue, and to investigate the effects of the knockdown of AFAP1-AS1 in TPC-1 papillary thyroid carcinoma cells on cell epithelial-mesenchymal transition (EMT) and related molecular mechanism in TPC-1 cells. Methods Real-time quantitative PCR was used to detect the expression of lncRNA AFAP1-AS1 in 60 cases of papillary thyroid carcinoma tissues. RNA interfering (RNAi) was used to knockdown AFAP1-AS1 in TPC-1 cells. TPC-1 cells were divided into AFAP1-AS1 knockdown (shAFAP1-AS1) group, negative control RNA (shNC) group and untransfected control group. The colony-formation assay, Transwell invasion and scratch healing assays were employed to detect the colony-forming ability, cell invasion ability and cell migration ability of TPC-1 cells, respectively. After knockdown of AFAP1-AS1, real-time quantitative PCR and Western blot analysis were used to detect the mRNA and protein levels of E-cadherin, vimentin, β-catenin and snail2, respectively. Results Compared with the paracancerous tissue, the expression level of AFAP1-AS1 mRNA in the papillary thyroid carcinoma tissue significantly increased. Knockdown of AFAP1-AS1 significantly reduced the colony-forming ability, invasion and migration ability of TPC-1 cells. Compared with shNC group and control group, knockdown of AFAP1-AS1 significantly reduced the mRNA and protein expression of snail2, vimentin and β-catenin. In contrast, the mRNA and protein expression of E-cadherin increased considerably. Conclusion The lncRNA AFAP1-AS1 is highly expressed in papillary thyroid carcinoma tissue. After knockdown of AFAP1-AS1 in TPC-1 cells, the colony-forming ability, invasion and migration ability of cancer cells are significantly down-regulated, which may be related to the inhibition of EMT.
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January 2021

Case Report: A Unique Case of Pediatric Central Nervous System Embryonal Tumor Harboring the - Fusion, Germline Variant and Somatic Mutation: Expanding the Spectrum of -Rearranged Neoplasia.

Front Oncol 2020 2;10:598970. Epub 2020 Dec 2.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China.

Central nervous system (CNS) embryonal tumors (WHO grade IV) are a heterogeneous group of rare, poorly differentiated neuroepithelial malignant neoplasms that commonly occur in children, and they have a poor prognosis. The 2016 WHO (World Health Organization) classification of CNS tumors created a major shift in paradigm of the classification of embryonal tumors. However, some cases were still difficult to classify. Further integrative genomic analysis is needed to improve the precise classification, diagnosis and treatment of CNS embryonal tumors. Herein, we firstly report a case of CNS embryonal tumor harboring the pathogenic - gene fusion. A 2-year-old male infant presented with a solid cystic mass in the left temporal lobe-basal ganglia and left parietal lobe (maximum diameter, 75 mm) and underwent gross tumor resection. The tumor was classified as a poorly differentiated embryonal neoplasm of neuroectodermal origin that lacked specific features and rosettes. By immunohistochemistry, the tumor cells were strongly positive for synaptophysin, and the Ki67 proliferation index was high (>50%). FISH (Fluorescence in situ hybridization) results indicated no change in the copy number at the 19q13.42 C19MC locus. Next generation sequencing showed a - gene fusion, a somatic c.G2714A mutation, and a heterozygous germline c.C127T mutation. One month after surgery, there was recurrence of the intracranial tumor (maximum diameter, 55 mm) as well as spinal cord implantation metastasis. The patient received chemotherapy (CTX+CBP+VCR/DDP+VP-16), radiotherapy, and a drug targeting the gene (everolimus). At the time of this writing, the patient is alive without evidence of disease for 11 months. This is the first report of the - gene fusion in a case of CNS embryonal tumor.
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http://dx.doi.org/10.3389/fonc.2020.598970DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738346PMC
December 2020

CHIC Risk Stratification System for Predicting the Survival of Children With Hepatoblastoma: Data From Children With Hepatoblastoma in China.

Front Oncol 2020 18;10:552079. Epub 2020 Nov 18.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Objective: The aim of this study was to compare the accuracy of the Children's Oncology Group (COG) risk stratification system to the Children's Hepatic tumor International Collaboration (CHIC) risk stratification system for predicting the prognosis of Chinese children with hepatoblastoma (HB).

Methods: Clinicopathological data of 86 patients diagnosed with HB between January 2014 and December 2017 were retrieved. The study endpoints were the 1- and 3-year overall survival (OS) and disease-free survival (DFS) were analyzed to evaluate the predictive value.

Results: The 1-, 3-year OS and DFS of the 86 patients were 86.0%, 76.3%, and 74.4%, 54.0%, respectively. Univariate analyses revealed that age at diagnosis had a significant role in prognosis for both OS and DFS, along with PRETEXT staging and metastasis at diagnosis. Multivariate analysis showed that metastasis at diagnosis (HR 3.628, 95% CI 1.404-9.378, P=0.008), PRETEXT staging system (HR 2.176, 95% CI 1.230-3.849, P=0.008) and age at diagnosis (HR 2.268, 95% CI 1.033-4.982, P=0.041) were independent factors for OS. For DFS, the independent factors were the PRETEXT staging system (HR 2.241, 95% CI 1.533-3.277, P<0.001) and age at diagnosis (HR 1.792, 95% CI 1.018-3.154, P=0.043). Both COG and CHIC risk stratification systems could effectively predict the prognosis of children with HB for OS. For DFS, the CHIC risk stratification system was more effective. In addition, the CHIC risk stratification system had a higher c-index (OS 0.743, DFS 0.730), compared to the COG risk stratification system (OS 0.726, DFS 0.594).

Conclusion: Age at diagnosis played a significant role in prognosis. Compared to the COG risk stratification system, the CHIC risk stratification system was superior in predicting the survival of Chinese children with HB.
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http://dx.doi.org/10.3389/fonc.2020.552079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708347PMC
November 2020

Bioinformatics Analysis and Validation Identify CDK1 and MAD2L1 as Prognostic Markers of Rhabdomyosarcoma.

Cancer Manag Res 2020 26;12:12123-12136. Epub 2020 Nov 26.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, 510060, People's Republic of China.

Purpose: The goal of the current study was to identify potential prognostic biomarkers of rhabdomyosarcoma (RMS).

Materials And Methods: We screened chip sequencing datasets of RMS through the gene expression omnibus (GEO) database. A total of 74 RMS patient tissues and 39 normal muscle cell tissues were analyzed. Limma R software was used to identify the differentially expressed genes (DEGs) between RMS tissues and normal controls. The GO plot R package was used to visualize the results of the GO analysis. We screened for pathaffy package enrichment of DEGs by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The cutoff criterion was a P-value <0.05. Immunohistochemistry (IHC) was applied to validate the expression of CDK1 (cyclin-dependent kinases 1) and MAD2L1 (Mitotic Arrest Deficient 2 Like 1) in RMS.

Results: We obtained a total of 498 up- and 480 down-regulated DEGs. The hub genes are mainly involved in the cell cycle and P53 singling pathway. CDK1 expression was associated with tumor size and COG-STS (Children's Oncology Group-soft tissue sarcoma) staging of RMS. For the low CDK1 expression group and high CDK1 expression group, the 5-year overall survival (OS) rate was 83.0% vs 63.5% (P = 0.004), and the 5-year event-free survival (EFS) rate was 47.5% vs 27.5% (P = 0.049) respectively. When compared low MAD2L1 expression group with high MAD2L1 expression group, the 5-year OS rate was 80.0% vs 43.2% (P = 0.001), and the 5-year EFS rate was 45.1% vs 21.8% (P = 0.038), respectively. If patients were divided into three groups: low CDK1 and low MAD2L1 expression group, high CDK1 or high MAD2L1 expression group, and high CDK1 and high MAD2L1 expression group, the 5-year OS rate was 87.1%, 58.6%, 39.6% (P = 0.001), while the 5-year EFS rate of RMS patients was 54.2%, 23.2%, 21.7% (P = 0.028), respectively.

Conclusion: This study has identified that CDK1 and MAD2L1 were adverse prognostic factors of RMS.
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http://dx.doi.org/10.2147/CMAR.S265779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705535PMC
November 2020

Maternal regulation of inflammatory cues is required for induction of preterm birth.

JCI Insight 2020 11 19;5(22). Epub 2020 Nov 19.

Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.

Infection-driven inflammation in pregnancy is a major cause of spontaneous preterm birth (PTB). Both systemic infection and bacterial ascension through the vagina/cervix to the amniotic cavity are strongly associated with PTB. However, the contribution of maternal or fetal inflammatory responses in the context of systemic or localized models of infection-driven PTB is not well defined. Here, using intraperitoneal or intraamniotic LPS challenge, we examined the necessity and sufficiency of maternal and fetal Toll-like receptor (TLR) 4 signaling in induction of inflammatory vigor and PTB. Both systemic and local LPS challenge promoted induction of inflammatory pathways in uteroplacental tissues and induced PTB. Restriction of TLR4 expression to the maternal compartment was sufficient for induction of LPS-driven PTB in either systemic or intraamniotic challenge models. In contrast, restriction of TLR4 expression to the fetal compartment failed to induce LPS-driven PTB. Vav1-Cre-mediated genetic deletion of TLR4 suggested a critical role for maternal immune cells in inflammation-driven PTB. Further, passive transfer of WT in vitro-derived macrophages and dendritic cells to TLR4-null gravid females was sufficient to induce an inflammatory response and drive PTB. Cumulatively, these findings highlight the critical role for maternal regulation of inflammatory cues in induction of inflammation-driven parturition.
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http://dx.doi.org/10.1172/jci.insight.138812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710297PMC
November 2020

Challenges and opportunities for managing pediatric central nervous system tumors in China.

Pediatr Investig 2020 Sep 27;4(3):211-217. Epub 2020 Sep 27.

Department of Oncology St. Jude Children's Research Hospital Memphis TN USA.

Central nervous system (CNS) tumors represent the most deadly cancer in pediatric age group. In China, thousands of children are diagnosed with CNS tumors every year. Despite the improving socioeconomic status and availability of medical expertise within the country, unique challenges remain for the delivery of pediatric neuro-oncology service. In this review, we discuss the existing hurdles for improving the outcome of children with CNS tumors in China. Need for precise disease burden estimation, lack of intra- and inter-hospital collaborative networks, high probability of treatment abandonment, along with financial toxicities from treatment represent the key challenges that Chinese healthcare providers encounter. The tremendous opportunities for advancing the status of pediatric neuro-oncology care in and beyond the country are explored.
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http://dx.doi.org/10.1002/ped4.12212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520110PMC
September 2020

Treatment of childhood cancer in China: Current status and future direction.

Pediatr Investig 2020 Sep 27;4(3):153-156. Epub 2020 Sep 27.

Department of Pediatric Oncology Sun Yat-Sen University Cancer Center Guangzhou China.

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http://dx.doi.org/10.1002/ped4.12210DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520102PMC
September 2020

Dietary supplementation with selenium-enriched earthworm powder improves antioxidative ability and immunity of laying hens.

Poult Sci 2020 Nov 8;99(11):5344-5349. Epub 2020 Aug 8.

Department of Ecology and Engineering, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.

Selenium (Se) has been recognized as an essential dietary nutrient for decades, and organic Se sources rather than inorganic ones are increasingly advocated as Se supplements. Earthworms have been studied as a feed additive and animal protein source for many yr. The aim of this study was to evaluate the effect of Se-enriched earthworm powder (SEP) on the antioxidative ability and immunity of laying hens. A total of 120 27-wk-old laying hens were randomly divided into 4 groups (30 hens per group). Laying hens were fed diets supplemented with SEP having 0, 0.5, or 1 mg/kg of Se or with earthworm powder alone. After 5 wk of supplementation, serum from the hens was tested for nutritional components (protein, globulin, albumin, triglycerides, total cholesterol, and glucose), antioxidative properties (glutathione peroxidase, superoxide dismutase, catalase, and nitric oxide), and immune responses (lysozymes, immunoglobulin G, IL-2, and interferon gamma). We found that SEP with 1.0 mg/kg of Se upregulated the hens' total protein, albumin, glutathione peroxidase, superoxide dismutase, IgG, and IL-2 and downregulated triglycerides, total cholesterol, glucose, and nitric oxide. These results indicate that SEP improves antioxidative levels and immune function of laying hens, indicating potential benefit from use of SEP as a feed additive in the poultry industry.
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http://dx.doi.org/10.1016/j.psj.2020.07.030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647737PMC
November 2020

Safety and diagnostic accuracy of percutaneous CT-guided transthoracic biopsy of small lung nodules (≤20 mm) adjacent to the pericardium or great vessels.

Diagn Interv Radiol 2021 Jan;27(1):94-101

Department of Interventional Radiology, Fudan University Shanghai Cancer Center, Shanghai, China.

Purpose: We aimed to evaluate the safety and diagnostic accuracy of computed tomography (CT)-guided transthoracic biopsy of small lung nodules (≤20 mm) adjacent to the pericardium or great vessels.

Methods: This retrospective study examined the safety and diagnostic accuracy of percutaneous CT-guided biopsy for small lung nodules (≤20 mm) located within 10 mm of the pericardium or great vessels. Technical aspects and factors influencing complications were assessed, and diagnostic accuracy was calculated.

Results: A total of 168 biopsies were performed in 168 patients. The complications were mainly pneumothorax (34.5%; 58 of 168 patients), chest tube insertion (5.3%; 9 of 168 patients), and pulmonary hemorrhage (61.3%; 103 of 168 procedures), with no patient mortality. One patient (0.6%) was admitted because of hemorrhage complications. Significant independent risk factors for pneumothorax were nodules resided in upper or middle lobes and lateral patient position, and for hemorrhage, longer distance from structures and longer needle trajectory through the lung parenchyma. Overall, the sensitivity, accuracy, and specificity were 91.0%, 92.2%, and 100%, respectively.

Conclusion: Percutaneous CT-guided transthoracic biopsy was highly accurate in small lung nodules (≤20 mm) adjacent to the pericardium or great vessels. Complications are common, but most were minor and self-limited.
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http://dx.doi.org/10.5152/dir.2020.20051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837726PMC
January 2021

Desorption of CO, SO, and NH in the vacuum evaporation of desulfurization wastewater.

Environ Sci Pollut Res Int 2021 Feb 2;28(6):6664-6674. Epub 2020 Oct 2.

National Engineering Laboratory for Reducing Emissions from Coal Combustion, Engineering Research Center of Environmental Thermal Technology of Ministry of Education, Shandong Key Laboratory of Energy Carbon Reduction and Resource Utilization, School of Energy and Power Engineering, Shandong University, Jinan, 250061, Shandong, China.

Mechanical vapor compression and multi-effect evaporation have been widely used in achieving zero discharge of desulfurization wastewater as they are energy-saving and efficient technologies. Solubilized weak ions, such as CO, SO, and NH, in the desulfurization wastewater are partly converted into CO, SO and NH, respectively, during the vacuum evaporation process, thus affecting the heat exchange and compressor performance. In this study, the migration and coupling mechanism of CO, SO, and NH desorption in desulfurized wastewater under vacuum evaporation were analyzed. The effects of temperature, pressure, reaction time, and other factors on the migration process were discussed. The hydrolysis and electrolytic equilibrium constants of the related ions were obtained for temperatures between 70 and 90 °C. The results demonstrate the relationship between the desorption capacities of CO, SO, and NH and the hydrolysis constants of their respective ions. The desorption of CO and NH increased significantly when CO and NH coexisted, whereas the SO desorption capacity remained low under the same experimental conditions. The experimental results indicate that the desorption of CO, SO, and NH is controlled by chemical reactions and can be described by first-order reaction kinetics.
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http://dx.doi.org/10.1007/s11356-020-10720-4DOI Listing
February 2021

Pregnancy success in mice requires appropriate cannabinoid receptor signaling for primary decidua formation.

Elife 2020 09 29;9. Epub 2020 Sep 29.

Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, United States.

With implantation, mouse stromal cells begin to transform into epithelial-like cells surrounding the implantation chamber forming an avascular zone called the primary decidual zone (PDZ). In the mouse, the PDZ forms a transient, size-dependent permeable barrier to protect the embryo from maternal circulating harmful agents. The process of decidualization is critical for pregnancy maintenance in mice and humans. Mice deficient in cannabinoid receptors, CB1 and CB2, show compromised PDZ with dysregulated angiogenic factors, resulting in the retention of blood vessels and macrophages. This phenotype is replicated in but not in mice. In vitro decidualization models suggest that levels substantially increase in mouse and human decidualizing stromal cells, and that neutralization of CB1 signaling suppresses decidualization and misregulates angiogenic factors. Taken together, we propose that implantation quality depends on appropriate angiogenic events driven by the integration of CB2 in endothelial cells and CB1 in decidual cells.
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http://dx.doi.org/10.7554/eLife.61762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7550189PMC
September 2020

CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway.

J Exp Clin Cancer Res 2020 Aug 26;39(1):169. Epub 2020 Aug 26.

Department of Obstetrics & Gynecology, Shengjing Hospital Affiliated to China Medical University, No. 36 Sanhao Street, Heping District, Shenyang City, 110004, Liaoning Province, China.

Background: Endometrial cancer (EC) is a common gynecologic malignancy worldwide. This study investigated the regulatory effects of circular RNA (circRNA) hsa_circ_0002577 on the tumorigenesis of EC.

Methods: Tumor samples and adjacent normal tissues were obtained from 84 EC patients. Recombinant lentiviral vectors expressing hsa_circ_0002577 (Lv-circRNA), short hairpin RNAs against hsa_circ_0002577 (sh-circRNA), miR-625-5p mimics, miR-625-5p inhibitor, lentiviral vectors expressing insulin-like growth factor 1 receptor (IGF1R) and their corresponding controls were transfected into EC cells as designated. A mouse xenograft model was established in BALB/c mice by inoculating Ishikawa cells transfected with sh-circRNA or control sequence.

Results: Hsa_circ_0002577 was upregulated in EC tissue samples and cells as compared to normal controls. EC patients with higher expression of hsa_circ_0002577 showed poorer overall survival and more advanced tumor stage. EC cells transfected with Lv-circRNA showed promoted proliferation, migration, and invasion, whereas the delivery of sh-circRNA exerted an opposite effect. Further analyses showed that hsa_circ_0002577 acted as a miR-625-5p sponge in EC cells. IGF1R was a potential downstream target of miR-625-5p. The expression of IGF1R in EC tissues was significantly higher than that in matched controls. Hsa_circ_0002577 accelerated EC development by inducing IGF1R expression and activating PI3K/Akt signaling pathway. Also, the knockdown of hsa_circ_0002577 delayed tumor growth and metastasis in the inoculated mice.

Conclusion: Our study showed that circRNA hsa_circ_002577 accelerated EC progression by acting as a miR-625-5p sponge, upregulating IGF1R and activating the PI3K/Akt pathway, suggesting the potential therapeutic use of hsa_circ_002577 in EC treatment.

Trial Registration: Not Applicable.
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http://dx.doi.org/10.1186/s13046-020-01679-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450704PMC
August 2020

The prognosis of prechemotherapy blastemal predominant histology subtype in Wilms tumor: A retrospective study in China.

Pediatr Blood Cancer 2020 11 19;67(11):e28567. Epub 2020 Aug 19.

Department of Pediatric Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong, P. R. China.

Purpose: This study aimed to retrospectively analyze survival outcomes for Chinese patients with prechemotherapy blastemal predominant histology type Wilms tumors (WTs).

Methods: We collected and analyzed clinical data concerning patients aged <15 years with favorable histology (FH) WTs treated at the Sun Yat-Sen University Cancer Center from December 2005 to May 2016, based on the Children's Oncology Group protocol. Pathological specimens were collected through biopsy or surgical resection before initiation of chemotherapy. We analyzed survival outcomes involving different prechemotherapy histology subtypes.

Results: We enrolled 97 patients with FH WTs (median follow-up, 71.5 months; range, 22.2-170.7). The total recurrence rate was 17.5%, and the subtype recurrence rates were as follows: blastemal predominant (45.5%), mixed (7.5%), epithelial (14.3%), and mesenchymal (9.5%) (P = .010). Five-year event-free survival (EFS) and overall survival (OS) rates were 84.9% and 81.4%, respectively. Respective 5-year EFS and OS rates for subtypes were as follows: blastemal predominant (54.5% and 68.2%), mixed (90.0% and 88.9%), epithelial (85.7% and 85.1%), and mesenchymal (90.5% and 94.7%). Multivariate survival analyses showed that the blastemal predominant subtype was an independent prognostic factor of EFS (P = .001) and OS (P = .017).

Conclusions: Our findings showed that prechemotherapy blastemal predominant WTs had higher recurrence and lower EFS and OS rates. Our findings suggested that, albeit with some deficiencies, blastemal predominant histology WT-diagnosed prechemotherapy may have prognostic relevance. Further research into other potential confounding variables are required to determine whether such patients warrant altered risk-stratified therapy.
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http://dx.doi.org/10.1002/pbc.28567DOI Listing
November 2020

The Efficacy and Safety of Apatinib in Refractory/Relapse Advanced Pediatric Solid Tumor: A Retrospective Study.

Cancer Manag Res 2020 22;12:6177-6185. Epub 2020 Jul 22.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.

Background: The prognosis of recurrent or refractory advanced childhood solid tumor patients is very poor and new therapeutic strategies are in urgent need. This study aimed to determine the efficacy and safety of apatinib in pediatric refractory/relapse advanced solid tumor patients.

Patients And Methods: The study retrospectively reviewed recurrent or refractory advanced pediatric solid tumor patients who were treated with apatinib, an oral small-molecule tyrosine kinase inhibitor (TKI) that targets vascular endothelial growth factor receptor-2 (VEGFR2), at the Sun Yat-sen University Cancer Center (China) from January 2016 to March 2019.

Results: Fifty-six patients were included in the safety evaluation and 49 patients were included in the efficacy evaluation. The objective responses rate (ORR) was 26.5% (95% CI 15-41): 0 CR (complete response) and 13 PR (partial response). Disease control rate (DCR) (CR+PR+SD) was 79.6% (95% CI 65-90). The median progression-free survival (PFS) was 4.0 months (95% CI 2.6-5.4). There was no significant difference for ORR or PFS between the A (apatinib monotherapy), A+MT (apatinib combined with oral metronomic therapy) and A+SC (apatinib combined with salvage combination chemotherapy) group (p>0.05). The most common grade 3 or 4 adverse events were neutropenia (9[16.1%]), thrombocytopenia (8[14.3%]), hand-foot syndrome (3[5.4%]), hypertension (3[5.4%]), anaemia (3[5.4%]) and mucositis (2[3.6%]). Hypertension was the most serious adverse event and one death that occurred was considered as drug-related.

Conclusion: Apatinib showed promising clinical activity in heavily treated recurrent or refractory advanced childhood solid tumor patients. However, it is necessary to pay special attention to monitoring blood pressure when using apatinib in children. Prospective randomized controlled clinical trial is warranted.
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http://dx.doi.org/10.2147/CMAR.S258689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383017PMC
July 2020

Synthesis and Reactivity of Bis(silylene)-Coordinated Calcium and Divalent Lanthanide Complexes.

Chemistry 2020 Nov 5;26(65):14888-14895. Epub 2020 Oct 5.

Institute of Inorganic Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstraße 15, 76131, Karlsruhe, Germany.

Divalent lanthanide complexes of Eu (1) and Yb (2) coordinated by a chelating pyridine-based bis(silylene) ligand were isolated and fully characterized. Compared to the Eu complex 1, the Yb complex 2 presents a lower thermal stability, resulting in the activation of one Si -N bond and formation of an Yb complex (3), which features a unique silylene-pyridyl-amido ligand. The different thermal stability of 1 and 2 points towards reduction-induced cleavage of one Si -N bond of the bis(silylene) ligand. Successful isolation of the corresponding redox-inert bis(silylene) Ca complex (5) was achieved at low temperature and thermal decomposition into a Ca complex (4) bearing the same silylene-pyridyl-amido ligand was identified. In this case, the thermolysis reaction proceeds through another, non-redox induced, mechanism. An alternative higher yielding route to 4 was developed through an in situ generated silylene-pyridyl-amine proligand.
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http://dx.doi.org/10.1002/chem.202003417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756572PMC
November 2020

An Integrated Microfluidic Probe for Mass Spectrometry Imaging of Biological Samples*.

Angew Chem Int Ed Engl 2020 12 9;59(50):22388-22391. Epub 2020 Oct 9.

Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.

Ambient ionization based on liquid extraction is widely used in mass spectrometry imaging (MSI) of molecules in biological samples. The development of nanospray desorption electrospray ionization (nano-DESI) has enabled the robust imaging of tissue sections with high spatial resolution. However, the fabrication of the nano-DESI probe is challenging, which limits its dissemination to the broader scientific community. Herein, we describe the design and performance of an integrated microfluidic probe (iMFP) for nano-DESI MSI. The glass iMFP, fabricated using photolithography, wet etching, and polishing, shows comparable performance to the capillary-based nano-DESI MSI in terms of stability and sensitivity; a spatial resolution of better than 25 μm was obtained in these first proof-of-principle experiments. The iMFP is easy to operate and align in front of a mass spectrometer, which will facilitate broader use of liquid-extraction-based MSI in biological research, drug discovery, and clinical studies.
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http://dx.doi.org/10.1002/anie.202006531DOI Listing
December 2020

Grape pomace inhibits colon carcinogenesis by suppressing cell proliferation and inducing epigenetic modifications.

J Nutr Biochem 2020 10 2;84:108443. Epub 2020 Jun 2.

School of Food Science, Washington State University, Pullman, WA, USA. Electronic address:

Grape pomace (GP), a by-product of the wine and juice industry, is rich in bioflavonoids and dietary fibers. We hypothesized that GP has protective effects against colitis-associated colorectal cancer (CRC). Nine-week-old female mice were fed a control diet (CON) or CON with 5% grape pomace (GP) for 2 weeks, when mice were subjected to azoxymethane (AOM)/dextran sulfate sodium (DSS) induced-CRC induction. GP supplementation ameliorated the disease activity index (DAI) score, reduced tumor number, tumor size and pathological scores in AOM/DSS treated mice. Furthermore, dietary GP suppressed colonic expression of inflammatory cytokines, IL-1β and TNF-α, and inhibited NF-κB inflammatory signaling, while increased anti-inflammatory cytokine TGF-β mRNA expression. Colorectal inflammation is known to enhance Wnt signaling and cell proliferation. In agreement, the content of β-catenin, a key downstream mediator of Wnt signaling, was reduced as was the expression of Cyclin D1, phosphorylation and content of p53 and PCNA level in GP-fed mice. In addition, GP reduced the expression of ALDH1, a marker of cell stemness, and increased the expression of Cdx2, a key transcription factor initiating epithelial cell differentiation, DNA methylation of the promoter region of Cdx2 gene and hypermethylation of CpG island methylator phenotype (CIMP), which commonly occurs during CRC carcinogenesis, was alleviated in the GP group. In conclusion, GP supplementation suppressed colitis-associated CRC carcinogenesis, which was associated with the suppression of inflammation and cell proliferation and the enhancement of DNA demethylation in Cdx2 and CIMP genes in the colon. These data suggest that dietary GP supplementation has preventive effects against colorectal carcinogenesis.
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http://dx.doi.org/10.1016/j.jnutbio.2020.108443DOI Listing
October 2020

Expression of Nogo-A in dorsal root ganglion in rats with cauda equina injury.

Biochem Biophys Res Commun 2020 06 28;527(1):131-137. Epub 2020 Apr 28.

Department of Orthopedic Surgery, Spine Center, Changzheng Hospital, Second Military Medical University, No.415 Fengyang Road, Shanghai, 200003, People's Republic of China. Electronic address:

Objective: To investigate the expression of Nogo-A in dorsal root ganglion (DRG) in rats with cauda equina injury and the therapeutic effects of blocking Nogo-A and its receptor.

Methods And Materials: Fifty-eight male Sprague-Dawley rats were divided randomly into either the sham operation group (n = 24) or the cauda equina compression (CEC) control group (n = 34). Behavioral, histological, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analyses were conducted to assess the establishment of the model. The dynamic expression change of Nogo-A was evaluated using real time-qPCR. Immunofluorescence was used to evaluate the expression of Nogo-A in the DRG and cauda equina. Furthermore, 20 male Sprague-Dawley rats were equally divided into 4 groups, including the sham group, the CEC group, the NEP1-40 (the NgR antagonist peptide) treatment group, and the JTE-013 (the S1PR2 antagonist) treatment group. Behavioral assessments and western blotting were used to evaluate the therapeutic effect of cauda equina injury via blocking Nogo-A and its receptor.

Results: Tactile allodynia and heat hyperalgesia in the CEC model developed as soon as 1 day after surgery and recovered to normal at 7 days, which was followed by the downregulation of Nogo-A in DRG neurons. However, the locomotor function impairment in the CEC model showed a different prognosis from the sensory function, which was consistent with the expression change of Nogo-A in the spinal cord. Immunofluorescence results also demonstrated that Nogo A-positive/NF200-negative neurons and axons increased in the DRG and cauda equina 7 days after surgery. Surprisingly, Schwann cells, which myelinate axons in the PNS, also expressed considerable amounts of Nogo-A. Then, after blocking the Nogo-A/NgR signaling pathway by NEP1-40, significant improvement of mechanical allodynia was identified in the first 2 days after the surgery. Western blotting suggested the NEP1-40 treatment group had lower expression of cleaved caspase-3 than the CEC and JTE-013 treatment group.

Conclusion: Neuronal Nogo-A in the DRG may be involved in regeneration and play a protective role in the CEC model. Whereas Nogo-A, released from the injured axons or expressed by Schwann cells, may act as an inhibiting factor in the process of CEC repairment. Thus, blocking the Nogo-A/NgR signaling pathway can alleviate mechanical allodynia by apoptosis inhibition.
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http://dx.doi.org/10.1016/j.bbrc.2020.04.094DOI Listing
June 2020

Rituximab is highly effective in children and adolescents with Burkitt lymphoma in Risk Groups R2 to R4.

Pediatr Hematol Oncol 2020 Sep 4;37(6):489-499. Epub 2020 May 4.

Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, China.

Data regarding the use of rituximab in children and adolescents with Burkitt's lymphoma (BL) is limited. This study retrospectively analyzed the effect of rituximab on children and adolescents with BL in risk group (R) 2 to R4. Patients underwent chemotherapy according to the revised NHL-BFM-95 protocol. Rituximab was administered at the dose of 375 mg/m on day 0 of each cycle. A total of 106 patients were included. Stratified by the number of doses of rituximab, there were 49, 16, and 41 patients in group 1 (no rituximab), group 2 (1-3 doses of rituximab) and group 3 (≥4 doses of rituximab), respectively. The 3-year event-free survival (EFS) rates were 83.2% (95% CI = 72.6%-93.8%), 81.2% (95% CI = 52.3%-93.5%) and 96.8% (95% CI = 78.8%-99.6%) in group 1, group 2 and group 3, respectively ( = 0.077). In R2/R3, the relapse rates were 0 in patients treated with rituximab and 11.8% in those treated without rituximab ( = 0.516). In R4, the relapse rates were 18.8%, 21.4% and 3.0% in group 1, group 2 and group 3, respectively ( = 0.048). Rituximab is highly effective in children and adolescents with BL in R2 to R4. The optimal number of doses was 4-6 in patients with BL in R4.
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http://dx.doi.org/10.1080/08880018.2020.1759741DOI Listing
September 2020

Cardiac motion non-influential in percutaneous computed tomography-guided biopsies of small (≤ 20 mm) lung nodules near pericardium.

Jpn J Radiol 2020 Sep 15;38(9):890-898. Epub 2020 Apr 15.

Department of Interventional Radiology, Fudan University Shanghai Cancer Center, No.270 Dongan Road, Xuhui, Shanghai, 200032, China.

Purpose: To assess the impact of cardiac motion during percutaneous computed tomography (CT)-guided core needle biopsy (PCT-CNB) of small lung lesions near pericardium, focusing on safety and diagnostic accuracy.

Materials And Methods: Seventy-eight PCT-CNBs were performed between March 2010 and June 2018 in 78 patients with small (≤ 20 mm) lung nodules, each within 10 mm of pericardium. Shifts in distance and length of interface separating lesions from pericardium were calculated and compared by cardiac chambers (left atrium, left ventricle, right atrium, or right ventricle). Risk factors for complications were subjected to univariate analysis, and diagnostic accuracy was assessed.

Results: The respective mean values were 0.8 ± 1.1 mm (range 0-5.1 mm) for shifts in distance and 1.5 ± 2.1 mm (range 0-10.8 mm) for length of interface. Neither parameter shifted significantly with respect to cardiac chambers (p > 0.05, both). Pneumothorax ensued in 28 patients (35.9%), and pulmonary hemorrhage occurred in 41 (52.6%). The overall sensitivity, specificity, and accuracy of PCT-CNB were 91.2%, 100%, and 93.2%, respectively.

Conclusion: Our data indicate that cardiac motion has no impact on either the incidence of complications or the diagnostic accuracy of PCT-CNB in patients with small (≤ 20 mm) lung lesions near pericardium.
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http://dx.doi.org/10.1007/s11604-020-00970-0DOI Listing
September 2020

Scribble promotes alveologenesis in the pregnant mammary gland for milk production.

Reproduction 2020 05;159(6):719-731

Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.

Mammary glands are comprised of ducts and terminal lobules that form tree-like structures. Luminal epithelial cells in these lobules undergo differentiation into alveolar cells in pregnancy to support milk production. This study reveals that Scribble (SCRIB), a scaffold protein expressed in progesterone receptor (PGR)-positive cells, plays a critical role in mammary gland alveologenesis in mice. We conditionally deleted Scrib using a Pgr-Cre driver. PGR is heterogeneously expressed throughout the luminal epithelium. Scrib loss in mammary glands by Pgr-Cre (Scribf/fPgrCre/+) shows inefficient alveologenesis and terminal end bud (TEB)-like morphology during pregnancy, resulting in poor milk production and subsequent death of pups after delivery. The differentiation of PGR-positive epithelial cells into Elf5-expressing alveolar cells is defective in Scribf/fPgrCre/+ mice. These changes are reflected in reduced activation of JAK2 and PAK1, resulting in downregulation of pSTAT5, a critical transcriptional factor for alveologenesis. These results provide evidence that SCRIB impacts PGR-positive cell lineage during alveologenesis, which impacts milk production and the health of offspring.
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http://dx.doi.org/10.1530/REP-20-0108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241298PMC
May 2020