Publications by authors named "Xiaofang Yu"

98 Publications

Integrated Strategy for Discovery and Validation of Glycated Candidate Biomarkers for Hemodialysis Patients with Cardiovascular Complications.

Anal Chem 2021 Mar 4. Epub 2021 Mar 4.

Shanghai Cancer Center and Department of Chemistry, Fudan University, Shanghai 200032, P. R.China.

Glycation plays a pathogenic role in many age-related degenerative pathological conditions, such as diabetes, end-stage renal diseases, and cardiovascular diseases. Mass spectrometry-based qualitative and quantitative analysis methods have been greatly developed and contribute to our understanding of protein glycation. However, it is still challenging to sensitively and accurately quantify endogenous glycated proteome in biological samples. Herein, we proposed an integrated and robust quantitative strategy for comprehensive profiling of early-stage glycated proteome. In this strategy, a filter-assisted sample preparation method was applied to reduce sample loss and improve reproducibility of sample preparation, contributing to high-throughput analysis and accurate quantification of endogenous glycated proteins with low abundance. Standard glycated peptides were spiked and performed the subsequent process together with complex samples both in label-free quantification and multiple reaction monitoring (MRM) analysis, contributing to the improvement of quantitative accuracy. In parallel, a novel approach was developed for the synthesis of heavy isotope-labeled glycated peptides used in MRM analysis. By this way, a total of 1128 endogenous glycated peptides corresponding to 203 serum proteins were identified from 60 runs of 10 pairs of hemodialysis patients with and without cardiovascular complications, and 234 glycated peptides corresponding to 63 proteins existed in >70% runs, among which 17 peptides were discovered to be differentially glycated ( < 0.05, fold-change > 1.5 or <0.67). Furthermore, we validated the glycation difference of four target peptides in 46 serum samples using MRM analysis, which were consistent with our results of label-free quantification.
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http://dx.doi.org/10.1021/acs.analchem.0c04028DOI Listing
March 2021

Moisture-sensitive metal-organic framework constructed from cobalt and 4-(4-pyridyl) benzoic acid for dispersive solid phase extraction of polycyclic aromatic hydrocarbons in apple.

J Sep Sci 2021 Feb 24. Epub 2021 Feb 24.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.

A moisture-sensitive metal-organic framework Co (pybz) ·2DMF was synthesized and applied as the adsorbent of dispersive solid phase extraction. The structure changed after water treatment due to the fact that two chelate carboxylate groups on the skeleton were transformed to monodentate because of the coordination of water molecules. The material showed good adsorption for fluorene, phenanthrene, fluoranthene and pyrene in water because of the π-π conjugation and π-complexation effects. Coupled with HPLC, a dispersive solid phase extraction method of determining the content of fluorene, phenanthrene, fluoranthene and pyrene in apple samples was established after optimizing the extraction conditions. Methanol containing 4% acetic acid was used as the effective eluent. The linearities were 0.5-1000 μg/kg for fluorene, phenanthrene and 5-1000 μg/kg for fluoranthene, pyrene. The limits of detection were 0.06-0.6 μg/kg, and the recoveries were 94.4-116.4%. The method has a high sensitivity for the determination of 4 polycyclic aromatic hydrocarbons in apple samples. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jssc.202001158DOI Listing
February 2021

Clinical Analysis of Cervical Lymph Node Metastasis Risk Factors and the Feasibility of Prophylactic Central Lymph Node Dissection in Papillary Thyroid Carcinoma.

Int J Endocrinol 2021 31;2021:6635686. Epub 2021 Jan 31.

Department of General Surgery, South Branch of Fujian Provincial Hospital, Fuzhou 350000, Fujian, China.

Objective: To identify the risk factors for cervical lymph node metastasis (CLNM) and the feasibility of prophylactic central lymph node dissection.

Methods: The characteristics of 1107 patients were extracted and analyzed. Univariate and multivariate analyses were used to identify risk factors associated with lymph node metastasis. The relationship between the central lymph node dissection (CLND) and lateral lymph node metastasis (LLNM) was analyzed using the correlation analysis.

Results: The probability of CLNM was closely related to the male gender, age <55, and the increase of tumor size. Those patients with an increase in tumor size and CLNM were extremely prone to LLNM. Also, LLNM was more likely to happen in those with the more positive central lymph nodes. Routine prophylactic central lymph node dissection (P-CLND) did not increase the risk of complications.

Conclusion: P-CLND should be considered as a reasonable surgical treatment for PTC.
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http://dx.doi.org/10.1155/2021/6635686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868159PMC
January 2021

Genistein Ameliorates Renal Fibrosis Through Regulation Snail via m6A RNA Demethylase ALKBH5.

Front Pharmacol 2020 19;11:579265. Epub 2020 Nov 19.

Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China.

Renal tubule-interstitial fibrosis is related to chronic kidney disease progression and a typical feature of the aging kidney. Epigenetic modifications of fibrosis-prone genes regulate the development of renal fibrosis. As a kind of "epigenetic diet", soy isoflavone genistein was reported to have renal protective action and epigenetic-modulating effects. However, its renal protection role and underlying mechanisms are yet to be fully clarified. Herein, we showed that genistein exhibits a demonstrable anti-fibrotic effect on kidney UUO (unilateral ureteral occlusion) model and renal epithelial cells model. The mechanism is strongly associated with epithelial-to-mesenchymal transition and m6A RNA demethylase ALKBH5. Mouse fibrotic kidneys induced by UUO exhibited adverse expression of renal fibrosis-related proteins and significant increases in the total m6A level. As an eraser, ALKBH5 showed severer suppression in the renal fibrosis process. However, genistein pretreatment restored ALKBH5 loss remarkably and reduced renal fibrosis, abnormal protein, and inflammatory markers. The examination of possible mechanisms revealed that genistein promoted ALKBH5 and maybe induced the level of mRNA m6A methylation in some epithelial-to-mesenchymal transition-related transcription factors. We found snail was the critical regulator and critical for the protective role of genistein. To verify the relationship between ALKBH5 and snail, we generated knockdown and overexpression of ALKBH5 cells . ALKBH5 knockdown enhanced the mesenchymal phenotype marker α-smooth muscle actin and snail expression. In agreement, overexpression ALKBH5 increased epithelial adhesion molecule E-cadherin and reduced snail expression. In conclusion, genistein increased renal ALKBH5 expression in UUO-induced renal fibrosis and reduced RNA m6A levels and ameliorates renal damages.
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http://dx.doi.org/10.3389/fphar.2020.579265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751752PMC
November 2020

Identification of the novel Np17 oncogene in human leukemia.

Aging (Albany NY) 2020 11 21;12(23):23647-23667. Epub 2020 Nov 21.

Department of Hematology, Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.

We previously defined the HERV-K Np9 as a viral oncogene. Here we report the discovery of a novel oncogene, Np17, which is homologous to the viral Np9 gene and predominantly present in Hominoidea. Np17 is located on chromosome 8, consists of 7 exons, and encodes a 16.8kDa nuclear protein with149 amino-acid residue. Functionally, knockdown of Np17 induced growth inhibition of leukemia cells, whereas enforced expression of Np17 promoted growth of leukemia cells in vitro and in vivo. In human leukemia, Np17 was detected in 59.65% (34/57) of acute myeloid leukemia (AML) patients examined and associated with refractory/relapsed AML. Mechanistically, Np17 decreased p53 levels and its mechanism might be involved in recruiting nuclear MDM2 to p53 for ubiquitin-mediated degradation. These findings reveal that Np17 is a novel oncogene associated with refractory/relapsed leukemia.
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http://dx.doi.org/10.18632/aging.103808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7762455PMC
November 2020

Application of Nano-Carbon Tracing Technology in Thyroid Cancer and Its Relationship with Cytotoxic T Lymphocyte Antigen 4 Gene Polymorphism.

J Nanosci Nanotechnol 2021 Feb;21(2):949-954

Department of Thyroid & Breast Surgery, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, Guangdong Province, China.

The most common malignant tumor of the human endocrine system is thyroid cancer, most used surgical treatment for thyroid cancer is total thyroidectomy with central lymph node dissection. However, surgery and thorough lymph node dissection can easily damage the parathyroid glands and cause corresponding surgery. Symptoms such as permanent hyperparathyroidism, nano-carbon is a common type of new lymphoid tissue tracer, it is mainly used to trace lymphoid tissue in tumor surgery. With the continuous advancement of surgical technology, more and more scholars have used nano-carbon to trace lymphoid tissue during thyroid cancer surgery, and have achieved good results. The results of this study show that thyroid cancer surgery with nano-carbon to negatively develop the parathyroid glands significantly reduces the incidence of serum PTH and blood calcium decline, and largely protects the parathyroid glands. Practice has also shown that the use of nano-carbon tracers is more thorough in lymph node dissection than thyroid cancer surgery without nano-carbon tracers.
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http://dx.doi.org/10.1166/jnn.2021.18635DOI Listing
February 2021

Wnt/β-catenin agonist BIO alleviates cisplatin-induced nephrotoxicity without compromising its efficacy of anti-proliferation in ovarian cancer.

Life Sci 2020 Dec 26;263:118672. Epub 2020 Oct 26.

Department of Nephrology, Zhongshan Hospital, Fudan University, China; Shanghai Medical Center for Kidney, China; Shanghai Key Laboratory of Kidney and Blood Purifcation, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China. Electronic address:

Aims: Cisplatin is an anticancer agent marred by nephrotoxicity. Limiting this adverse effect may allow the use of higher doses to improve its efficacy. The Wnt/β-catenin signaling pathway plays a critical role in nephrogenesis and repair of renal diseases. BIO, a small molecule agonist of this pathway, exerted a protective effect in adriamycin nephropathy and promoted nephrogenesis. The aim of this study, therefore, was to investigate whether Wnt/β-catenin agonist BIO could protect against cisplatin-induced nephrotoxicity in vivo and in vitro, as well as its possible mechanism.

Main Methods: Male mice and human renal proximal tubular cells (HK-2) were subjected to cisplatin to study reno-protective effect of BIO. Renal function, cell viability, tubular apoptosis, production of reactive oxygen species (ROS) and proliferative level were analyzed respectively. Additionally, xenograft model was induced to investigate if BIO would impair the antitumor effect of cisplatin.

Key Findings: Cisplatin increased serum creatinine levels and promoted histological renal injury as well as oxidative stress levels. Besides, renal apoptotic level and the expression of pro-apoptotic proteins, Bax/bcl-2 and cleaved-caspase3 included, in the kidney were increased. All these features were decreased by BIO, which also activated Wnt/β-catenin pathway in cisplatin-induced nephrotoxicity. Similarly, accompanied by the motivation of Wnt/β-catenin pathway, BIO exerted a positively protective effect on HK-2 challenged cisplatin. Last, the chemotherapeutic effects of cisplatin in xenograft mice of ovary tumor models and in lung cancer cells weren't compromised by BIO.

Significance: Wnt/β-catenin agonist BIO has the potential to prevent cisplatin nephrotoxicity without compromising its anti-proliferation efficacy.
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http://dx.doi.org/10.1016/j.lfs.2020.118672DOI Listing
December 2020

Automatic microbial electro-Fenton system driven by transpiration for degradation of acid orange 7.

Sci Total Environ 2020 Jul 6;725:138508. Epub 2020 Apr 6.

Department of Chemistry and Chemical Engineering and Nanofiber Engineering Center of Jiangxi Province, Jiangxi Normal University, Ziyang Road 99th, 330022 Nanchang, China. Electronic address:

Microbial electro-Fenton system (MEFS) shows potential application for degradation of recalcitrant pollutants. In order to simplify the MEFS and adapt to the practical application situations, such as water, soil or sludge remediation, we developed an automatic MEFS (AMEFS) for degradation of a recalcitrant dye, acid orange 7. The AMEFS contained a microchannel-structured carbon decorated with iron oxides as electro-Fenton cathode. The AMEFS could be either two-electrode configuration that the microchannel-structured carbon connected with an additional bioanode by an external circuit, or single-electrode configuration that the microchannel-structured carbon served as both bioanode and cathode. Thanks to the microchannel structure of the carbon cathode, the AMEFS could be auto-driven by a process similar to the transpiration process of natural plants. The two-electrode AMEFS had higher degradation efficiency of acid orange 7 at lower external resistance, and achieved the highest degradation efficiency of 96% at the short-circuit condition. The single-electrode configuration simplified the setup of the AMEFS and possessed comparable performance with that of two-electrode configuration at short-circuit condition. Moreover, it could degrade high concentration acid orange 7 of up to 50 mg L and achieve a high degradation efficiency of over 93%. The AMEFS could be applied for soil and sludge remediation by direct insertion of the microchannel structured carbon into contaminated body.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138508DOI Listing
July 2020

Discharge pharmacotherapy for Type 2 diabetic inpatients at two hospitals of different tiers in Zhejiang Province, China.

PLoS One 2020 8;15(4):e0230123. Epub 2020 Apr 8.

Department of Endocrinology in Ningbo Medical Treatment Centre Lihuili Hospital, Ningbo, Zhejiang Province, China.

Objects: To look into the discharge pharmacotherapy for type 2 diabetics admitted to two general hospitals of different ranks and inspect current real-world management of discharge pharmacology and its related factors.

Methods: Type 2 diabetics admitted to a tertiary general hospital (Ningbo Medical Treatment Centre Lihuili Hospital, LHLH) or a secondary general hospital (Simen Hospital, SMH) for intensification of their anti-diabetics were included for retrospective analysis. Patients' demographics, clinical characteristics, admission diabetes therapy and discharge diabetes pharmacology were analyzed and compared among patients in each hospital as well as between two hospitals.

Results: 391 patients from LHLH and 164 patients from SMH were included for analyzing. Compared with patients from LHLH, patients from SMH were older, more illiterate and had higher HbA1c concentrations. While there was a nearly equal split of oral anti-diabetes drugs (OADs)-only and Insulin treatment in LHLH's discharge pharmacotherapy, insulin treatment dominated SMH's. Basal-and-bolus insulin assumed the majority of SMH's insulin regimens but only accounted for less than 20% of LHLH's. The principal discrepancy in OADs-only treatment existed in the utilization of newer classes of OADs. Cost and body mass index (BMI) were the main differentiating factors among OADs-only treatments while duration, BMI and HbA1c differ among insulin treatments at LHLH. Clinical characteristics didn't significantly differ among OADs-only treatments and HbA1c was the only differentiating factor among insulin treatments at SMH. Overall, hospital, duration, HbA1c, and vascular diseases were main factors that affect discharge pharmacology.

Conclusions: Great disparities exist in the discharge pharmacotherapy at two hospitals. Diabetes management is mostly glucose-oriented at SMH while multifactorial considerations were reflected in LHLH's discharge pharmacotherapy. Besides differences in patients' demographics, medication availability and diagnosis of early-stage vascular complications, lack of practical algorithm for discharge management in T2DM may be the underlying deficiency and a key part for future improvement.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230123PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141672PMC
June 2020

The presence of zinc reduced cadmium uptake and translocation in Cosmos bipinnatus seedlings under cadmium/zinc combined stress.

Plant Physiol Biochem 2020 Jun 19;151:223-232. Epub 2020 Mar 19.

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, 611130, China. Electronic address:

Cadmium (Cd) and zinc (Zn) coexist in the environment but interact differently in plants. Cosmos bipinnatus has been potentially considered as a Cd-accumulator. Thus, this study investigated the detoxification mechanism in C. bipinnatus seedlings under Cd, Zn and Cd + Zn stresses. In the present study, the presence of Zn inhibited Cd uptake and translocation, whereas Cd merely hindered Zn uptake. The concentration of Cd in soluble fraction significantly decreased and Cd was bounded to the cell wall in root under Cd + Zn stress. Meanwhile, Zn and Cd mutually decreased their concentrations in the ethanol extractable form (F) and water extractable form (F) in roots and shoots. Furthermore, Cd + Zn stress enhanced the activities of superoxide dismutase (SOD, EC 1.15.1.1), peroxidase (POD, EC 1.11.1.7) and catalase (CAT, EC 1.11.1.6) compared to Cd stress alone. These results suggested that Zn effectively decreased Cd uptake and translocation, changed their subcellular distributions, regulated their chemical forms composition and increased antioxidative enzyme activities, thereby enhancing the tolerance to Cd in C. bipinnatus. This study physiologically revealed the interactive effect of Cd and Zn on the detoxification mechanism of Cd in C. bipinnatus and provided new information on phytoremediation of the heavy metal contaminated soils.
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http://dx.doi.org/10.1016/j.plaphy.2020.03.019DOI Listing
June 2020

Superhydrophilic and highly elastic monolithic sponge for efficient solar-driven radioactive wastewater treatment under one sun.

J Hazard Mater 2020 06 19;392:122350. Epub 2020 Feb 19.

Nuclear Waste and Environmental Safety Key Laboratory of Defense, State Key Laboratory of Environment-friendly Energy Materials, National Co-innovation Center for Nuclear Waste Disposal and Environmental Safety, Sichuan Civil-military Integration Institute, School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, China. Electronic address:

As an effective way to obtain solar energy and separate the soluble contaminants from water, solar-driven interfacial evaporation is used in desalination, wastewater treatment, electricity generation, and domestic water heating system. Herein, we demonstrate a monolithic sponge with three-dimensional porous structure as the solar-energy evaporator, which is composed of hydrophilic polymer (Konjac Glucomannan, KGM) and solar absorbent (reduced graphene oxide, rGO). Under one sun irradiation, the sponge achieves a rapid evaporation rate (1.60 kg m h) and high interfacial water evaporation efficiency (92 %) due to its good absorption, photothermal, thermal insulation, and fast water transport properties. Meanwhile, the concentrations of radioactive elements (strontium, cesium, and uranium) in wastewater dropped from grams to micrograms after purification, even under radiation and acidic conditions. Additionally, the durability and repeatability of the sponge also have been verified. The results showed that solar-driven interfacial evaporation can effectively treat radioactive wastewater and enrich various radionuclides in a more energy-saving manner.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122350DOI Listing
June 2020

Different strategies for lead detoxification in dwarf bamboo tissues.

Ecotoxicol Environ Saf 2020 Apr 20;193:110329. Epub 2020 Feb 20.

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address:

Dwarf bamboo Sasa argenteostriata (Regel) E.G. Camus is considered as potential plants for metal phytoremediation in previous filed observations. However, the mechanisms of lead (Pb) detoxification has not been described. The objective of this study was to explore the difference strategies or mechanisms of Pb detoxification in plant tissues. In this regard, four Pb treatments with hydroponics including 0 (control), 300, 600, and 900 mg L were conducted to examine subcellular compartmentalization, Pb accumulation/species and antioxidant-assisted chelation. Our findings showed the retention of Pb by the whip-root system is one of its detoxification mechanisms to avoid damage the shoots. In addition, the cell wall retention is the dominant detoxification strategy of whips, new roots, old roots and new/old stems, while vacuolar compartmentalization is for new/old leaves. Interestingly, four low-mobility/-toxicity Pb species (i.e., FNaCl, FHAc, FHCl and FR) are distributed in roots, whips and stems, while two high-mobility/-toxicity Pb species (FE and FW) in leaves. The conversion of Pb to low-toxicity/-migration is a Pb-detoxification strategy in roots, whips and stems but not in leaves. Besides, the new/old roots and leaves can alleviate Pb damage through the synthesis of non-protein thiol, glutathione and phytochelatins. Among these, phytochelatins play a leading role in the detoxification in new/old roots, while glutathione is in new/old leaves. This study provides the first comprehensive evidence regarding the different strategies for Pb detoxification in dwarf bamboo tissues from physiological to cellular level, supporting that this plant could be rehabilitated for phytoremediation in Pb-contaminated media.
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http://dx.doi.org/10.1016/j.ecoenv.2020.110329DOI Listing
April 2020

Enhanced coupling through π-stacking in imidazole-based molecular junctions.

Chem Sci 2019 Nov 16;10(43):9998-10002. Epub 2019 Sep 16.

Department of Chemistry , Queensborough Community College of the City University of New York , Bayside , New York 11364 , USA . Email:

We demonstrate that imidazole based π-π stacked dimers form strong and efficient conductance pathways in single-molecule junctions using the scanning-tunneling microscope-break junction (STM-BJ) technique and density functional theory-based calculations. We first characterize an imidazole-gold contact by measuring the conductance of imidazolyl-terminated alkanes (, = 3-6). We show that the conductance of these alkanes decays exponentially with increasing length, indicating that the mechanism for electron transport is through tunneling or super-exchange. We also reveal that π-π stacked dimers can be formed between imidazoles and have better coupling than through-bond tunneling. These experimental results are rationalized by calculations of molecular junction transmission using non-equilibrium Green's function formalism. This study verifies the capability of imidazole as a Au-binding ligand to form stable single- and π-stacked molecule junctions at room temperature.
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http://dx.doi.org/10.1039/c9sc03760hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6979055PMC
November 2019

Physiological Basis of Heterosis for Nitrogen Use Efficiency of Maize.

Sci Rep 2019 12 10;9(1):18708. Epub 2019 Dec 10.

College of Agronomy, Inner Mongolia Agricultural University, Hohhot, 010019, China.

Efficient use of nitrogen inputs for concurrent improvements in grain yield and nitrogen use efficiency (NUE) has been recognized as a viable strategy for sustainable agriculture development. Yet, there is little research on the possible physiological basis of maize hybrid heterosis for NUE and measurable traits that are corresponding to the NUE heterosis. A field study was conducted for two years to evaluate the heterosis for NUE and determine the relationship between NUE and its physiological components. Two commercial hybrids, 'Xianyu335' and 'Zhengdan958', and their parental inbred lines, were grown at 0 (0 N) and 150 kg N ha (150 N), in a randomized complete block design with four replications each year. Compared to their parental lines, both hybrids displayed a significant heterosis, up to 466%, for NUE. N internal efficiency (NIE) accounted for 52% of the variation in heterosis for NUE, while there was generally negligible heterosis for nitrogen recovery efficiency (NRE). Heterosis for NIE and thereby for NUE in maize was ascribed to (i) an earlier establishment of pre-anthesis source for N accumulation, which phenotypically exhibited as a faster leaf appearance rate with higher maximum LAI and photosynthetic nitrogen use efficiency; (ii) a larger amount of N being remobilized from the vegetative tissues, especially from leaves, during the grain filling. Phenotypically, there was notably a rapid reduction in post-anthesis specific weights of leaf and stalk, but with maintained functionally stay-green ear leaves; and (iii) a higher productive efficiency per unit grain N, which was characterized by a reduced grain N concentration and enhanced sink strength.
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http://dx.doi.org/10.1038/s41598-019-54864-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904739PMC
December 2019

Unique root exudate tartaric acid enhanced cadmium mobilization and uptake in Cd-hyperaccumulator Sedum alfredii.

J Hazard Mater 2020 02 6;383:121177. Epub 2019 Sep 6.

College of Resources, Sichuan Agricultural University, Chengdu 611130, China. Electronic address:

Low molecular weight organic acids (LMWOA) involved in heavy metal tolerance, translocation, and accumulation in plants. However, underlying mechanism of LMWOA secretion in metal mobilization and uptake in hyperaccumulator still need to be identified. In this study, a C labeling rhizobox was designed to investigate the composition and distribution of LMWOA in the rhizosphere of S. alfredii. The result showed that about 2.30%, 2.25% and 2.35% of the assimilated C was incorporated into oxalic acid, malic acid, and tartaric acid in rhizosphere of S. alfredii after CO assimilation, respectively. Oxalic acid, malic acid, and tartaric acid were the predominant LMWOA in rhizosphere soil solution of hyperaccumulating ecotype (HE) S. alfredii, however, almost no tartaric acid was detected for non-hyperaccumulating ecotype (NHE). Tartaric acid was identified as the unique root exudate from HE S. alfredii which was mainly distributed within the range of rhizosphere 0-6 mm. Tartaric acid significantly increased the solubility of four Cd minerals. HE S. alfredii treated with tartrate + CdCO had higher Cd contents and larger biomass than CdCO treatment. Cadmium accumulation in HE S. alfredii was promoted by the exudation of tartaric acid, which was highly efficient in Cd solubilization due to the formation of soluble Cd-tartrate complexes.
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http://dx.doi.org/10.1016/j.jhazmat.2019.121177DOI Listing
February 2020

The Transareola Endoscopic Approach Yields Adequate Oncological Dissection as Open Approach in the Treatment of Papillary Thyroid Carcinoma.

Surg Laparosc Endosc Percutan Tech 2019 Dec;29(6):489-492

Department of Thyroid Surgery, Second Medical Clinical College of Jinan University, Shenzhen People's Hospital, Shenzhen.

Objective: This study aimed to evaluate and discuss whether the transareola endoscopic surgery has similar outcome outcomes to open surgery in the treatment of papillary thyroid carcinoma (PTC).

Method: A total of 102 patients with PTC were enrolled in this study. Among them, 53 patients were treated by transareola endoscopic surgery (endoscopic group) and 49 patients were treated by open surgery (open group). Some specific factors, including thyroglobulin (Tg), radioactive iodine uptake (RAIU), postoperative nuclide imaging in thyroid area, postoperative nuclide imaging of lymph nodes suspicious for metastasis (PNILNSM), etc. were analyzed and compared between the 2 groups.

Results: There were no significant differences between the 2 groups regarding body mass index (22.9±3.4 vs. 24.0±3.3, P=0.103), operation time (173.3±43.2 vs. 158.8±47.9 min, P=0.110), intraoperative blood loss (41.8±19.4 vs. 35.8±31.0 mL, P=0.251, P=0.251), tumor diameter (19.0±6.8 vs. 20.2±7.2 mm, P=0.400), and overall complications (11.3% vs. 10.2%, P=0.868). No significant difference was found in the specific factors between the 2 groups concerning RAIU-2h/24h (2.44±1.34 vs. 2.58±1.65%/2.83±3.75 vs. 2.35±3.44%, P=0.646/ P=0.506), number of dissected lymph nodes (4.4±1.4 vs. 4.6±1.5, P=0.595), Tg before radioiodine therapy (4.46±5.50 vs. 5.60±8.36; P=0.495), Tg after radioiodine therapy (1.03±1.93 vs. 1.11±1.61, P=0.812, P=0.812), postoperative nuclide imaging in thyroid area (1.76±1.50 vs. 2.19±1.85 cm, P=0.195), PNILNSM before radioiodine (none: 79.2% vs. 83.7%, P=0.566; central: 17.0% vs. 12.2%, P=0.653; lateral: 1.9% vs. 4.1%, P=0.450; central+lateral: 1.9% vs. 0%, P=1.000), and PNILNSM after radioiodine (none: 94.3% vs. 95.9%, P=0.111; central: 3.8% vs. 2.0%, P=1.000; lateral: 0 vs. 2.0%, P=0.480; central+lateral: 1.9% vs. 0%, P=1.000).

Conclusions: Transareola endoscopic total thyroidectomy and central lymph nodes dissection are safe and effective. According to the evaluated postoperative specific factors, this technique achieves similar outcomes to open surgery in selected patients with PTC.
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http://dx.doi.org/10.1097/SLE.0000000000000729DOI Listing
December 2019

Aberrant activation of RPB1 is critical for cell overgrowth in acute myeloid leukemia.

Exp Cell Res 2019 11 28;384(2):111653. Epub 2019 Sep 28.

Department of Hematology and Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310009, China; Institute of Hematology, Zhejiang University, Hangzhou, 310009, China. Electronic address:

Acute myeloid leukemia (AML) is a group of highly aggressive malignancies with a 5-year overall survival of less than 40%. Cell overgrowth with defective apoptosis is a hallmark of AML, but little is known about how it occurs. Here, we show that aberrant activation of the largest subunit of RNA polymerase II (RPB1) encoded by POLR2A gene is critically involved in this hallmark. We retrospectively analyzed the expression profiles of POLR2A and RPB1 in a panel of AML cell lines, primary AML patients and peripheral blood samples. Meanwhile, correlation analysis was used to explore the correlation between the expression of RPB1 with tumor burden and overall survival time in untreated AML samples. RNA-Seq approach was performed to identify the differentially expressed genes between RPB1 silencing AML cells with control cells after knocking out RPB1. Furthermore, orthotopic AML models were established with RPB1 silencing and control cells to investigate the effects of RPB1 protein level on leukemia cell growth. In most AML patients, RPB1 was aberrantly activated and closely associated with poor prognosis, but not in normal hematopoietic cells. Global transcriptomic analysis revealed that POLR2A knockout strongly impaired growth of AML cells by selectively depleting a substantial set of AML-related oncogenic and anti-apoptosis genes such as MYC, RUNX2, MEIS1, CDC25A and BCL-2. Silencing RPB1 by genetic technology led to a potent regression of human refractory AML in mouse models. These findings reveal that dysregulated RPB1 is a central oncogenic hub that drives overgrowth by hijacking an array of oncogenic and anti-apoptosis factors. Targeting RPB1 is a potential therapeutic for treating AML.
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http://dx.doi.org/10.1016/j.yexcr.2019.111653DOI Listing
November 2019

Defective modulation of LINE-1 retrotransposition by cancer-associated SAMHD1 mutants.

Biochem Biophys Res Commun 2019 11 4;519(2):213-219. Epub 2019 Sep 4.

The First Hospital of Jilin University, Institute of Virology and AIDS Research & Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China; Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, Ministry of Education), Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address:

Long interspersed nuclear elements (LINE-1) is now considered as the only active autonomous mobile DNA in humans, LINE-1 retrotransposition activities are associated with and fluctuate during cancer initiation and progression; however, the mechanism underlying the increased LINE-1 activity in cancer is poorly understood. SAMHD1 has been reported to be a potent inhibitor of LINE-1 retrotransposition, and SAMHD1 mutations are frequently associated with cancer development. To gain insights on whether cancer-related SAMHD1 mutants affect LINE-1 activity, we explored the biochemical and cellular properties of some human mutants known correlate with the development of cancer. Most of the tested SAMHD1 cancer-related mutations were defective in LINE-1 inhibition. Interestingly we also found that SAMHD1 mutant K288T was defective for dNTPase activity but showed potent activity against LINE-1 retrotransposition. These findings suggest that LINE-1 inhibition does not depend solely on the dNTPase activity of SAMHD1. In contrast, SAMHD1's ability to inhibit ORF2p-mediated LINE-1 RNP reverse transcription was correlated with SAMHD1-mediated LINE-1 inhibition. Together, our data could also facilitate the deeper understanding for the inhibition of endogenous LINE-1 elements by SAMHD1.
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http://dx.doi.org/10.1016/j.bbrc.2019.08.155DOI Listing
November 2019

The difference of T cell phenotypes in end stage renal disease patients under different dialysis modality.

BMC Nephrol 2019 08 5;20(1):301. Epub 2019 Aug 5.

Department of Nephrology, Zhongshan Hospital, Fudan University, NO180, Feng'lin Road, Shanghai, 200032, China.

Background: Impaired T cell immune function exists in end-stage renal disease (ESRD) patients. Dialysis treatment may lead to changes in T cell subsets. In the present study, we aimed to identify alterations of T cell phenotypes in ESRD patients, especially in those receiving peritoneal dialysis (PD), and analyze the potential associated factors.

Methods: In the present study, 110 PD patients and 110 age/gender-matched hemodialysis (HD) patients who met the inclusion criteria were studied. Pre-dialysis blood samples were obtained and analyzed by flow cytometry to detect the expression of CD45RO and CCR7. Univariate and multivariate regression analyses were used to determine the factors associated with the alteration of T cell phenotypes.

Results: In all dialysis patients, age was associated with the frequencies of both CD4+ and CD8+ naïve T cells, effector memory (EM) T cells and effector memory RA (EMRA) T cells but not central memory (CM) T cells. Dialysis modality was also associated with T cell subsets. Compared with HD patients, PD patients showed an increase in both CD4+ and CD8+ CM T cells and a reduction in both CD4+ and CD8+ EM and EMRA T cells. However, the number of CD4+ naïve T cells was lower and the number of CD8+ naïve T cells was higher in PD patients than those in HD patients. In PD patients, further multivariate analysis revealed that the frequency of CD4+ naïve T cells was positively associated with nPCR, while the frequency of CD8+ naïve T cells was negatively associated with age.

Conclusion: In dialysis patients, the dialysis modality and age influence T cell subsets. There is a progression from naïve to effector T cells in HD patients compared with PD patients. In PD patients, different factors may influence the frequencies of CD4+ and CD8+ naïve T cells.
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http://dx.doi.org/10.1186/s12882-019-1475-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683494PMC
August 2019

Autologous decellularized extracellular matrix protects against HO-induced senescence and aging in adipose-derived stem cells and stimulates proliferation .

Biosci Rep 2019 05 21;39(5). Epub 2019 May 21.

Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, West Xueyuan Road, Lucheng Direct, Wenzhou City, Zhejiang Province, People's Republic of China

Adipose-derived stem cells have attracted significant interest, especially in stem cell therapy and regenerative medicine. However, these cells undergo gradual premature senescence in long-term cultures, which are essential for clinical applications that require cell-assisted lipotransfer or tissue repair. Since the extracellular matrix forms the microenvironment around stem cells and regulates self-renewal and multipotency in part by slowing down stem cell aging, we evaluated its potential to protect against senescence, using HO-induced adipose-derived stem cells as a model. We found that supplementing cultures with decellularized extracellular matrix harvested from the same cells significantly promotes proliferation and reverses signs of senescence, including decreased multipotency, increased expression of senescence-associated β-galactosidase, and accumulation of reactive oxygen species. These findings suggest a novel approach in which an autologous decellularized extracellular matrix is used to prevent cellular senescence to enable the use of adipose-derived stem cells in regenerative medicine.
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http://dx.doi.org/10.1042/BSR20182137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527929PMC
May 2019

Serum Concentration of Indoxyl Sulfate in Peritoneal Dialysis Patients and Low-Flux Hemodialysis Patients.

Blood Purif 2019 30;48(2):183-190. Epub 2019 Apr 30.

Division of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China,

Background/aims: To compare the serum concentration of Indoxyl sulfate (IS) in patients on continuous ambulatory peritoneal dialysis (CAPD) and low-flux hemodialysis (HD), and analyze the risk factors associated with IS.

Methods: We performed a single-center, cross-sectional observational study including 169 patients on CAPD and 115 patients on low-flux HD. Patients were divided into the anuric HD group, anuric peritoneal dialysis (PD) group, and non-anuric PD group on the basis of dialysis modality and residual urinary output. Serum concentration of IS was determined by high-performance liquid chromatography electrospray tandem mass spectrometry.

Results: After matching the urinary volume and dialysis vintage, 58 anuric patients on PD and 58 anuric patients on HD were enrolled. The serum level of IS was significantly lower in patients on PD than that in those on HD (28.05 ± 13.98 vs. 39.64 ± 18.25 μg/mL; p < 0.001). This result persisted even after adjustment for confounding risk factors including nutritional status (β = 0.338, p < 0.001). In addition, the serum level of IS was significantly lower in non-anuric PD patients than that anuric PD patients (18.70 ± 11.21 vs. 28.05 ± 13.98 μg/mL; p < 0.001). After the adjustment for risk factors such as dialysis vintage, IS serum concentration in patients on PD was still significantly correlated with residual renal function (RRF; β = -0.355, p < 0.001).

Conclusions: Dialysis modality is the independent risk factor of IS serum concentration and it is substantially lower in patients on CAPD than that in those on low-flux HD. Additionally, RRF was independently associated with IS serum concentration in CAPD patients, and the better the RRF is, the lower IS serum concentration.
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http://dx.doi.org/10.1159/000499749DOI Listing
January 2020

An integrated strategy for high-sensitive and multi-level glycoproteome analysis from low micrograms of protein samples.

J Chromatogr A 2019 Aug 15;1600:46-54. Epub 2019 Apr 15.

Department of Chemistry and Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address:

Glycosylation, as a biologically important protein post-translational modification, often alters on both glycosites and glycans, simultaneously. However, most of current approaches focused on biased profiling of either glycosites or glycans, and limited by time-consuming process and milligrams of starting protein material. We describe here a simple and integrated spintip-based glycoproteomics technology (termed Glyco-SISPROT) for achieving a comprehensive view of glycoproteome with shorter sample processing time and low microgram starting material. By carefully integrating and optimizing SCX, C18 and Concanavalin A (Con A) packing material and their combination in spintip format, both predigested peptides and protein lysates could be processed by Glyco-SISPROT with high efficiency. More importantly, deglycopeptide, intact glycopeptide and glycans released by multiple glycosidases could be readily collected from the same Glyco-SISPROT workflow for LC-MS analysis. In total, above 1850 glycosites in ˜1770 unique deglycopeptides were characterized from mouse liver by using either 100 μg of predigested peptides or directly using 100 μg of protein lysates, in which about 30% of glycosites were released by both PNGase F and Endos. To the best of our knowledge, this approach should be one of the most comprehensive glycoproteomic approaches by using limited protein starting material. One significant benefit of Glyco-SISPROT is that whole processing time is dramatically reduced from a few days to less than 6 h with good reproducibility when protein lysates were directly processed by Glyco-SISPROT. We expect that this method will be suitable for multi-level glycoproteome analysis of rare biological samples with high sensitivity.
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http://dx.doi.org/10.1016/j.chroma.2019.04.041DOI Listing
August 2019

Kidney injury molecule-1 expression predicts structural damage and outcome in histological acute tubular injury.

Ren Fail 2019 Nov;41(1):80-87

a Department of Nephrology , Zhongshan Hospital, Shanghai Medical College, Fudan University , Shanghai , China.

Background: A few studies have shown that urinary kidney injury molecule-1 (uKIM-1) levels are increased in acute kidney injury (AKI); however, the correlation between uKIM-1 and histological tubular injury, which is considered to be the gold standard for evaluating renal damage and predicting prognosis, is still unclear. We performed this study to determine whether the predicted value of uKIM-1 is correlated with renal KIM-1 (tKIM-1) expression and tissue damage in AKI patients.

Methods: This retrospective study recruited 14 healthy individuals and 27 biopsy-proven acute tubular injury (ATI) patients. uKIM-1 and plasma KIM-1 (sKIM-1) levels were measured by ELISA, and tKIM-1 expression was evaluated by immunohistochemistry.

Results: Elevated levels of urinary, plasma, and renal KIM-1 were found in ATI patients. The uKIM-1 concentration was positively correlated with tKIM-1 expression and reflected the severity of renal histological injury. The outcome of ATI was associated with uKIM-1 expression: the ATI patients with higher uKIM-1 levels had an increased potential for an incomplete recovery of renal function during follow-up. Additionally, the level of KIM-1, regardless of source, was negatively related to the eGFR, and ROC curve analysis revealed that the ROC-AUC was 0.923 (p = 0.000) for the diagnosis of ATI based on a combination of high uKIM-1 and sKIM-1 levels.

Conclusion: The uKIM-1 level corresponds with the severity of renal histological damage and can be a potential reliable predictor of adverse renal outcomes in ATI patients. Moreover, combining uKIM-1 and sKIM-1 can increase the sensitivity and specificity of the diagnosis of severe ATI.
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http://dx.doi.org/10.1080/0886022X.2019.1578234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6442099PMC
November 2019

Transforming growth factor-β1-overexpressing mesenchymal stromal cells induced local tolerance in rat renal ischemia/reperfusion injury.

Cytotherapy 2019 05 23;21(5):535-545. Epub 2019 Jan 23.

Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China; Shanghai Medical Center for Kidney, Shanghai, China; Shanghai Institute of Kidney and Dialysis, Shanghai, China. Electronic address:

Background: Regulatory T cells (Tregs) suppress excessive immune responses and play a crucial protective role in acute kidney injury (AKI). The aim of this study was to examine the therapeutic potential of transforming growth factor (TGF)-β1-overexpressing mesenchymal stromal cells (MSCs) in inducing local generation of Tregs in the kidney after ischemia/reperfusion (I/R) injury.

Methods: MSCs were transduced with a lentiviral vector expressing the TGF-β1 gene; TGF-β1-overexpressing MSCs (designated TGF-β1/MSCs) were then transfused into the I/R-injured kidney via the renal artery.

Results: MSCs genetically modified with TGF-β1 achieved overexpression of TGF-β1. Compared with green fluorescent protein (GFP)/MSCs, TGF-β1/MSCs markedly improved renal function after I/R injury and reduced epithelial apoptosis and subsequent inflammation. The enhanced immunosuppressive and therapeutic abilities of TGF-β1/MSCs were associated with increased generation of induced Tregs and improved intrarenal migration of the injected cells. Futhermore, the mechanism of TGF-β1/MSCs in attenuating renal I/R injury was not through a direct canonical TGF-β1/Smad pathway.

Conclusion: TGF-β1/MSCs can induce a local immunosuppressive effect in the I/R-injured kidney. The immunomodulatory activity of TGF-β1-modified MSCs appears to be a gateway to new therapeutic approaches to prevent renal I/R injury.
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http://dx.doi.org/10.1016/j.jcyt.2018.12.003DOI Listing
May 2019

The orphan nuclear receptor RORα is a potential endogenous protector in renal ischemia/reperfusion injury.

FASEB J 2019 04 23;33(4):5704-5715. Epub 2019 Jan 23.

Department of Nephrology, Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Emerging evidence indicates that retinoid-related orphan receptor (ROR)α, a member of the ROR nuclear receptor subfamily, mediates key cellular adaptions to hypoxia and contributes to the pathophysiology of many disease states. However, the effects of RORα in renal ischemia/reperfusion (I/R) injury remain unclear. Wild-type (WT) C57 black 6 (C57BL/6) mice and RORα-deficient stagger [ROR(sg/sg)] mice and their WT littermates were used for in vivo studies. The renal I/R injury model was induced by bilateral renal pedicle clamping for 35 min. Human proximal tubule cell line cells were treated with hypoxia (1% oxygen) to establish the cell hypoxia/reoxygenation (H/R) model. We investigated the renal expression and biologic function of RORα, and we found that RORα was significantly down-regulated after renal I/R injury. ROR(sg/sg) mice displayed dramatically augmented renal dysfunction and morphologic damage compared with WT mice at 24 h post-I/R. Further study revealed that the detrimental effects of RORα deficiency were attributable to tubular epithelial cell apoptosis and, consequently, renal inflammation and oxidative stress. The proapoptotic effect of RORα deficiency was associated with aggravated mitochondrial dysfunction in renal tubular cells after I/R. However, pretreatment of C57BL/6 mice with the RORα agonist SR1078 ameliorated I/R-induced renal dysfunction and damage and elicited a concomitant decrease in tubular epithelial cell apoptosis. In summary, our study provides experimental evidence showing that RORα is a novel endogenous protector against renal I/R injury and that ROR-α activation is a promising therapeutic strategy for the prevention of acute kidney injury.-Cai, J., Jiao, X., Fang, Y., Yu, X., Ding, X. The orphan nuclear receptor RORα is a potential endogenous protector in renal ischemia/reperfusion injury.
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http://dx.doi.org/10.1096/fj.201802248RDOI Listing
April 2019

Bacterial community composition shaped by water chemistry and geographic distance in an anthropogenically disturbed river.

Sci Total Environ 2019 Mar 17;655:61-69. Epub 2018 Nov 17.

School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China.

'Core bacterial communities', bacterial species that are found consistently throughout a river continuum, have previously been identified. However, variations in core and non-core bacterial community structure, as well as the relationships between these communities and water chemistry or geographic distance have not been well studied. Here, we sampled in the entire course of the Le'an River, China, and explored the bacterial community composition at each site using Illumina high-throughput sequencing. The proportion of sequence reads assigned to the core community was ~95% in the upper and middle reaches, gradually decreasing below 90% in the lower reaches. Both the Chao1 richness index and the Shannon diversity index of the bacterial communities were significantly higher in the wet season than in the dry season, and both indices increased slightly from upstream to downstream. The variation in the non-core community was more aggregated from upstream to downstream in the wet season than in the dry season, while the aggregation of the core community was similar between the dry season and the wet season. The proportion of typical freshwater bacterial was significantly higher in the core community than in the non-core community. NO-N was the subset of water chemistry parameters that best explained bacterial community dissimilarities, while 'river length' was the subset of geographic distance parameters that best explained bacterial community dissimilarities. Water chemistry parameters explained more of the variations in the bacterial communities than did geographic distance, especially in the dry season. However, the correlation between water chemistry and bacteria was primarily due to collective allochthonous input (mass effects), not because of any nutritious or toxic effects on bacterial growth competition (species sorting). The greater influence of the mass effects, as compared to species sorting, on bacterial community structure was due to the allochthonous input of bacteria from anthropogenic sources.
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http://dx.doi.org/10.1016/j.scitotenv.2018.11.234DOI Listing
March 2019

Newer Perspectives of Mechanisms for Euglycemic Diabetic Ketoacidosis.

Int J Endocrinol 2018 2;2018:7074868. Epub 2018 Oct 2.

Department of Endocrinology, Ningbo Medical Center Lihuili Hospital, Ningbo 315040, China.

Euglycemic diabetic ketoacidosis (EDKA) was considered a rare condition with its specific definition and precipitating factors. However, with the wide use of sodium glucose cotransporter 2 (SGLT-2) inhibitors, the newest class of antidiabetic agents, EDKA has come back into the spotlight. Relevant cases are increasingly being reported along with insights into the mechanism of EDKA. It seems increasingly clear that EDKA is more common than we used to believe. The SGLT-2 inhibitor-associated EDKA also indicates a necessary review of our previous understanding of "diabetic" ketoacidosis, since the SGLT-2 inhibitor predisposes patients to DKA in a "starvation" way. Actually, there are growing reports about starvation-induced ketoacidosis as well. The previously "exclusive" nomenclature and cognition of these entities need to be reexamined. That the hormonal interactions in DKA may differ from the severity of insulin deficiency also may have served in the scenario of EDKA. The SGLT-2 inhibitors are newly approved in China. The main purpose of this work is to have a better understanding of the situation and update our knowledge with a focus on the pathogenesis of EDKA.
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http://dx.doi.org/10.1155/2018/7074868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6189664PMC
October 2018

Natural polymer konjac glucomannan mediated assembly of graphene oxide as versatile sponges for water pollution control.

Carbohydr Polym 2018 Dec 5;202:425-433. Epub 2018 Sep 5.

State Key Laboratory of Environmentally Friendly Energy Materials, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, PR China; Sichuan Co-Innovation Center for New Energetic Material, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, PR China. Electronic address:

Three-dimensional network structure of konjac glucomannan/graphene oxide (KGM/GO) sponges was successfully prepared by ice template method. The KGM/GO sponges was rich in functional groups, negatively charged under pH 2 to 10. Batch adsorption experiment was conducted to investigate the adsorption performance of the as-prepared KGM/GO sponges for organic dye (malachite green (MG)) and radionuclide (uranium U(Ⅵ)). The results showed that the maximum adsorption capacities of KGM/GO sponges were 266.97, 189.96 mg/g for U(Ⅵ) and MG, respectively. Moreover, the KGM/GO sponges exhibited an excellent selectivity for capturing U(Ⅵ) in multi-ion system. The adsorption process was fitted better to pseudo-second order model, while adsorption isotherms for these pollutants were well matched up to Langmuir models. In addition, KGM/GO sponges can be easily separated from the aqueous solution and could be effectively reused for 5 times without obvious loss in adsorption performance. The advantages of eco-friendliness, low cost, simple preparation process, controllable shape and size, as well as high adsorption capacities for MG and U(Ⅵ), suggested that KGM/GO sponges promising in water pollution control.
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http://dx.doi.org/10.1016/j.carbpol.2018.08.133DOI Listing
December 2018

Stromal vascular fraction cells plus sustained release VEGF/Ang-1-PLGA microspheres improve fat graft survival in mice.

J Cell Physiol 2019 05 21;234(5):6136-6146. Epub 2018 Sep 21.

First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Autologous fat transplantation is increasingly applied in plastic and reconstructive surgery. Stromal vascular fraction cells (SVFs) combined with angiogenic factors, such as VEGF (vascular endothelial growth factor A) and Ang-1 (angiogenin-1), can improve angiogenesis, which is a critical factor for graft survival. However, direct transplant with such a mixture is insufficient owing to the short half-life of angiogenic factors. In this study, we evaluated whether a double sustained release system of VEGF/ANG-1-PLGA (poly (lactic-co-glycolic acid)) microspheres plus SVFs can improve angiogenesis and graft survival after autologous fat transplantation. VEGF/ANG-1-PLGA-sustained release microspheres were fabricated by a modified double emulsion-solvent evaporation technique. Human aspirated fat was mixed with SVF suspension plus VEGF/ANG-1 sustained release microspheres (Group C), SVF suspension (Group B) alone, or Dulbecco's modified Eagle's medium as the control (Group A). Eighteen immunocompromised nude mice were injected with these three mixtures subcutaneously at random positions. After 8 weeks, the mean volume of grafts was greater in the SVFs plus VEGF/ANG-1-PLGA group than in the control and SVFs groups (1.08 ± 0.069 ml vs. 0.62 ± 0.036 ml, and 0.83 ± 0.059 ml, respectively). Histological assessments showed that lower fibrosis, but greater microvascular density in the SVFs plus VEGF/ANG-1-PLGA group than in the other groups, though the SVFs group also had an appropriate capillary density and reduced fibrosis. Our findings indicate that SVFs plus VEGF/ANG-1-PLGA-sustained release microspheres can improve angiogenesis and graft survival after autologous fat transplantation.
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http://dx.doi.org/10.1002/jcp.27368DOI Listing
May 2019

Colorectal cancer-derived small extracellular vesicles establish an inflammatory premetastatic niche in liver metastasis.

Carcinogenesis 2018 12;39(11):1368-1379

Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province, China), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Liver metastases develop in more than half of the patients with colorectal cancer (CRC) and are associated with a poor prognosis. The factors influencing liver metastasis of CRC are poorly characterized, but this information is urgently needed. We have now discovered that small extracellular vesicles (sEVs; exosomes) derived from CRC can be specifically targeted to liver tissue and induce liver macrophage polarization toward an interleukin-6 (IL-6)-secreting proinflammatory phenotype. More importantly, we found that microRNA-21-5p (miR-21) was highly enriched in CRC-derived sEVs and was essential for creating a liver proinflammatory phenotype and liver metastasis of CRC. Silencing either miR-21 in CRC-sEVs or Toll-like receptor 7 (TLR7) in macrophages, to which miR-21 binds, abolished CRC-sEVs' induction of proinflammatory macrophages. Furthermore, miR-21 expression in plasma-derived sEVs was positively correlated with liver metastasis in CRC patients. Collectively, our data demonstrate a pivotal role of CRC-sEVs in promoting liver metastasis by inducing an inflammatory premetastatic niche through the miR-21-TLR7-IL-6 axis. Thus, sEVs-miR-21 represents a potential prognostic marker and therapeutic target for CRC patients with liver metastasis.
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http://dx.doi.org/10.1093/carcin/bgy115DOI Listing
December 2018