Publications by authors named "Xiaodong Zhou"

361 Publications

Relationship between age, refractive errors and motor fusion in a normal Chinese adult population: a cross-sectional study.

BMC Ophthalmol 2021 Sep 23;21(1):345. Epub 2021 Sep 23.

Department of Ophthalmology, Jinshan Hospital of Fudan University, 201508, Shanghai, China.

Background: To investigate the relationships between motor fusion and sex, age and spherical equivalent (SE).

Methods: This observational study enrolled 243 healthy, nonstrabismic adults, including 94 men and 149 women aged 20 to 59 years. The subjects were divided into three groups according to SE: myopic, emmetropic and hyperopic groups. The subjects were also divided into four groups according to age: 20-29, 30-39, 40-49 and 50-59 years groups. Motor fusion was measured with a synoptophore, including subjective angle (SA), divergence, convergence and fusional vergence range (FVR).

Results: The mean values of divergence, convergence and FVR for the whole sample group were 9.72 ± 0.26°, 19.34 ± 0.54°, and 29.06 ± 0.62°, respectively. A higher value of divergence was found in the myopic group than in the emmetropic group (p < 0.05). SE and divergence were significantly different among age groups (all p < 0.05). In addition, linear regression analysis showed that SE was correlated with divergence (p = 0.003). Age was correlated with SE, divergence and FVR (p < 0.001, p = 0.005, p = 0.002, respectively). In addition, the proportion of SA being in the comfort zone (defined as the value of SA satisfying Percival's criterion) in the age groups was significantly different (χ = 8.283, p = 0.041).

Conclusions: Motor fusion is associated with age and SE in the normal Chinese adult population.
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http://dx.doi.org/10.1186/s12886-021-02105-zDOI Listing
September 2021

Experimental Study on Thermal Runaway Process of 18650 Lithium-Ion Battery under Different Discharge Currents.

Materials (Basel) 2021 Aug 22;14(16). Epub 2021 Aug 22.

State Key Laboratory of Fire Science, University of Science and Technology of China, Hefei 230026, China.

Lithium-ion batteries (LIBs) subjected to external heat may be prone to failure and cause catastrophic safety issues. In this work, experiments were conducted to investigate the influence of discharge current on the thermal runaway process under thermal abuse. The calibrated external heat source (20 W) and discharge currents from 1 to 6 A were employed to match the thermal abuse conditions in an operational state. The results indicated that the key parameters during the failure process, such as the total mass loss, the onset temperatures of safety venting and thermal runaway, and the peak temperature, are ultimately determined by the capacity inside the battery, and the discharge current can hardly change it. However, discharge currents can produce extra energy to accelerate the thermal runaway process. Compared with the battery in an open circuit, the onset time of thermal runaway was reduced by 7.4% at 6 A discharge. To quantify the effect of discharge current, the total heat generation by discharge current was calculated. The results show that a heat generation of 1.6 kJ was produced when the battery was discharged at 6 A, which could heat the cell to 34 °C (neglect of heat loss). This study simulates the failure process of the LIB in the operational state, which is expected to help the safety application of LIB and improve the reliability of the battery management system.
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http://dx.doi.org/10.3390/ma14164740DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402224PMC
August 2021

Causes and Five-Year Proportion of New Irreversible Visual Impairment in Jinshan District, Shanghai, from 2009-2018.

J Ophthalmol 2021 28;2021:8873283. Epub 2021 Jul 28.

Department of Ophthalmology, Jinshan Hospital of Fudan University, Shanghai, China.

Purpose: To describe the age distribution and main causes of new registered irreversible visual impairment (VI) and to compare the five-year proportion of VI in Jinshan district, Shanghai, from 2009 to 2018.

Methods: The new irreversible VI data were collected in the registry system from the Disabled Persons' Federation in Jinshan district from January 1, 2009, to December 31, 2018. Age, gender, and causes of VI were included, and the 5-year proportion of VI was calculated.

Results: The peak occurrence of blindness occurred in the 50-59 yrs group in 2009-2013 and in the ≥70 yrs group in 2014-2018. The peak occurrence of low vision occurred in the 40-49 yrs group in 2009-2013 and in the 50-59 yrs group in 2014-2018. Myopic macular degeneration (MMD, 15.5%), diabetic retinopathy (DR, 14.3%), and other optic nerve atrophy (ONA, 14.3%) were the three leading causes of blindness in 2009-2013, whereas MMD (21.3%), age-related macular degeneration (AMD, 19.6%), ONA (14.9%) were the three leading causes of blindness in 2014-2018. MMD (39.2%), DR (9.6%), ONA (8.8%) were the three leading causes of low vision in 2009-2013, whereas MMD (38.7%), AMD (23.3%), ONA (7.4%) were the three leading causes of low vision in 2014-2018. The proportions of blindness and low vision caused by AMD were higher in 2014-2018 than those in 2009-2013 (=0.034 and < 0.001, respectively).

Conclusion: The present study demonstrated an increasing trend in the number of irreversibly visually impaired individuals from 2009 to 2018. More attention should be paid to people with high myopia and old age.
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http://dx.doi.org/10.1155/2021/8873283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8337108PMC
July 2021

Long ncRNA MALAT1 promotes cell proliferation, migration, and invasion in prostate cancer via sponging miR-145.

Transl Androl Urol 2021 Jun;10(6):2307-2319

Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China.

Background: The long non-coding (lncRNA) RNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) is known to promote tumorigenesis, whereas microRNA-145 (miR-145) plays an antitumor role in several cancers. In this study, we aimed to elucidate the role of MALAT1 and miR-145 in prostate cancer cells and investigate the effect of MALAT1 downregulation on prostate cancer (PCa) cells .

Methods: The Cancer Genome Atlas (TCGA) datasets were used to carry out the initial bioinformatics analysis; the findings were then tested in LNCaP and CWR22Rv1 cell lines. Western blot and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to evaluate the levels of MALAT1 and miR-145 along with related biomarkers. Furthermore, wound-healing and Transwell assays were performed to test the migratory and invasive abilities of PCa cells. Luciferase reporter assays were used to validate the relationship between MALAT1 and miR-145; their down-stream target genes were also studied. To further substantiate these findings in an animal model, tumor studies including immunofluorescence staining of tissues were carried in nude mice.

Results: The expression of MALAT1 was upregulated in both LNCaP cell lines and CWR22Rv1 cell lines (F=2.882, t=13.370, P<0.001; F=2.268, t=15.859, P<0.001). Knockdown of MALAT1 reduced the migratory and invasive capabilities of PCa cells (F=0.017, t=12.212, P<0.001; F=10.723, t=6.016, P=0.002). Using direct binding, MALAT1 suppressed the antitumor function of miR-145, which in turn upregulated transforming growth factor-β1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) via SMAD3 and TGFBR2 (F=2.097, t=5.389, P=0.006; F=1.306, t=4.155, P=0.014).

Conclusions: We confirmed that MALAT1 acts as a competing endogenous RNA (ceRNA) of miR-145. The MALAT1 based regulation of MiR-145-5p-SMAD3/TGFBR2 interactions could be an intriguing molecular pathway for the progression of PCa.
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http://dx.doi.org/10.21037/tau-20-1526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261405PMC
June 2021

Characterization of chondroitin sulfates isolated from large hybrid sturgeon cartilage and their gastroprotective activity against ethanol-induced gastric ulcers.

Food Chem 2021 Nov 20;363:130436. Epub 2021 Jun 20.

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address:

Sturgeon cartilage, which is rich in chondroitin sulfate (CS), is usually discarded during sturgeon utilization. In this paper, CS was isolated from large hybrid sturgeon skull and backbone and named SCS and BCS, respectively. Their structures were investigated via Fourier transform infrared (FT-IR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy and high performance liquid chromatography (HPLC). The average molecular weights of SCS and BCS were ~ 30-44 kDa. Disaccharide analysis indicated that SCS and BCS had similar chemical structures and were composed of ΔUA-[1 → 3]-GalNAc (ΔDi0S, 14.71%, 16.04%), ΔUA-[1 → 3]-GalNAc-4 s (ΔDi4S, 32.01%, 37.78%) and ΔUA-[1 → 3]-GalNAc-6 s (ΔDi6S, 53.27%, 46.18%). The gastroprotective effect of SCS and BCS were studied using a rat model of ethanol-induced gastric ulcers. Both SCS and BCS had apparent gastroprotective activity and their ulcer inhibition rate reached ~ 35%-45%, which was similar to that of omeprazole (~41%). These results provide useful strategies for the high-value utilization of sturgeon cartilage.
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http://dx.doi.org/10.1016/j.foodchem.2021.130436DOI Listing
November 2021

Circ_0001686 Promotes Prostate Cancer Progression by Up-Regulating SMAD3/TGFBR2 miR-411-5p.

World J Mens Health 2021 May 18. Epub 2021 May 18.

Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.

Purpose: As the mechanism of interaction between circular RNAs (circRNAs) and microRNAs (miRNAs) in regulating the development of prostate cancer (PCa) is not clear, this study focuses on investigating these effects.

Materials And Methods: Sample tissues were collected from the PCa of patients, and microarray analysis of human circRNAs was conducted. The expression of circ_0001686, has_miR-411-5p (miR-411-5p) were also detected by qRT-PCR. Circ_0001686 and miR-411-5p mimics were transfected into the PCa cell lines (CWR22RV1and LNCaP) and MTT, colony formation, Transwell, and scratch wound assays were used to analyze the biological behaviors of PCa cells. Si-circ_0001686 and ASO-miR-411-5p were used as negative controls, and dual-luciferase reporter assays were performed to verify the interactions among circ_0001686, miR-411-5p, and SMAD3/TGFBR2. The levels of SMAD3 and TGFBR2 in different treated PCa cells were measured by western blot, and experiments in a nude mouse model were carried out to strengthen the findings of miR-411-5p.

Results: The expression of circ_0001686 was up-regulated, while the expression of miR-411-5p was down-regulated in PCa cells. Moreover, circ_0001686 promoted cell proliferation, migration, and invasion. Molecular mechanism exploration revealed that circ_0001686 could reduce miR-411-5p, affecting the downstream target genes of SMAD3 and TGFBR2. and studies verified that miR-411-5p inhibits PCa progression.

Conclusions: Circ_0001686 can reduce miR-411-5p to increase the expression of SMAD3/TGFBR2, which consequently promotes the proliferation, invasion, and migration of PCa cells.
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http://dx.doi.org/10.5534/wjmh.200204DOI Listing
May 2021

Gold-silver nanoparticles modified electrochemical sensor array for simultaneous determination of chromium(III) and chromium(VI) in wastewater samples.

Chemosphere 2021 Oct 15;281:130880. Epub 2021 May 15.

Residues and Resource Reclamation Centre (R3C), Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Clean Tech One, 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore. Electronic address:

The oxidation state of ions is a crucial aspect that often has been overlooked when determining the toxicity of chromium (Cr) species in environmental samples. In this study, a novel electrochemical sensor array based on gold-silver nanoparticles modified electrodes was developed for simultaneous determination of the two main chromium species (Cr(III) and (VI)). Specifically, the working electrodes of screen-printed carbon electrodes (SPCEs) were modified with silver-gold bimetallic nanoparticles through electrochemical deposition for detection of Cr(VI). The silver-gold bimetallic nanoparticles were further oxidized to form stable silver-gold bimetallic oxide nanoparticles for the detection of Cr(III). The results showed that the addition of silver with a theoretical value of 1% of gold could contribute to the formation and stabilization of oxides on the surface of gold nanoparticles. After characterization, the two kinds of electrodes were integrated as an electrochemical sensor array for selective and sensitive detection of Cr(VI) and Cr(III). The linear range and limit of detection (LOD, identified by three times of signal-to-noise ratio) were found to be 0.05-5 ppm and 0.1 ppb for Cr(VI), and 0.05-1 ppm and 0.1 ppb for Cr(III), respectively. Finally, the electrochemical sensor array was proven for successful detection of Cr(VI) and Cr(III) in tap water, artificial saliva and artificial sweat samples, and monitoring of Cr(VI) and Cr(III) in chromium-containing wastewater treatment process. Combined with a handheld dual-channel electrochemical device, the simultaneous determination of Cr(VI), Cr(III) and total chromium contents can be easily achieved for various samples.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130880DOI Listing
October 2021

A prognostic nomogram for long-term major adverse cardiovascular events in patients with acute coronary syndrome after percutaneous coronary intervention.

BMC Cardiovasc Disord 2021 05 22;21(1):253. Epub 2021 May 22.

Department of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China.

Background: Accurate prediction of major adverse cardiovascular events (MACEs) is very important for the management of acute coronary syndrome (ACS) patients. We aimed to construct an effective prognostic nomogram for individualized risk estimates of MACEs for patients with ACS after percutaneous coronary intervention (PCI).

Methods: This was a prospective study of patients with ACS after PCI from January 2013 to July 2019 (n = 2465). After removing patients with incomplete clinical information, a total of 1986 patients were randomly divided into evaluation (n = 1324) and validation (n = 662) groups. Predictors included in the nomogram were determined by a multivariate Cox proportional hazards regression model based on the training set. Receiver operating characteristic (ROC) curves and calibration curves were used to assess the discrimination and predictive accuracy of the nomogram, which were then compared with those of the classic models. The clinical utility of the nomogram was assessed by X-tile analysis and Kaplan-Meier curve analysis.

Results: Independent prognostic factors, including lactate level, age, left anterior descending branch stenosis, right coronary artery stenosis, brain natriuretic peptide level, and left ventricular ejection fraction, were determined and contained in the nomogram. The nomogram achieved good areas under the ROC curve of 0.712-0.762 in the training set and 0.724-0.818 in the validation set and well-fitted calibration curves. In addition, participants could be divided into two risk groups (low and high) according to this model.

Conclusions: A simple-to-use nomogram incorporating lactate level effectively predicted 6-month, 1-year, and 4-year MACE incidence among patients with ACS after PCI.
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http://dx.doi.org/10.1186/s12872-021-02051-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141252PMC
May 2021

Delayed pericardial effusion after left atrial appendage closure with the LAmbre device: Importance of a fully open umbrella.

J Cardiovasc Electrophysiol 2021 06 12;32(6):1646-1654. Epub 2021 Apr 12.

Department of Cardiology, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Wenzhou, P.R. China.

Introduction: We aimed to investigate whether a modified implantation method facilitating a fully open umbrella can reduce the pericardial effusion/pericardial tamponade (PE/PT) rate after left atrial appendage closure (LAAC) with the LAmbre device compared with the conventional method (CM) in patients with non-valvular atrial fibrillation (NVAF).

Methods And Results: Patients with NVAF who received either isolated LAAC or combined catheter ablation and LAAC using the LAmbre device at the First Affiliated Hospital of Wenzhou Medical University from January 2018 to December 2019 were enrolled. CM was used for device implantation in the initial 59 patients, while a modified method (MM) was used in the remaining 165 patients. Successful implantation was achieved in 98.3% of patients in the CM group and 98.8% in the MM group. A higher rate of a fully open umbrella (98.8% vs. 69%, p < .001), less requirement for recapture (46% vs. 62.1%, p = .036), and a lower incidence of delayed PE/PT (1.2% vs. 8.6%, p = .005) were found in the MM group compared with the CM group. All of the five delayed PT events occurred in patients with combined treatment. An umbrella that was not fully open was the only factor associated with delayed PE/PT events in a multivariable Cox model.

Conclusions: LAAC with the LAmbre device using an MM significantly increases the rate of a fully open umbrella and decreases the requirement for recapture and the incidence of delayed PE/PT. This method is more effective in patients with combined treatment.
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http://dx.doi.org/10.1111/jce.15020DOI Listing
June 2021

Simple and green synthesis of calcium alginate/AgCl nanocomposites with low-smoke flame-retardant and antimicrobial properties.

Cellulose (Lond) 2021 Mar 22:1-17. Epub 2021 Mar 22.

College of Chemistry and Chemical Engineering, Shandong Collaborative Innovation Center of Marine Biobased Fibers and Ecological Textiles, Qingdao University, Qingdao, 266071 China.

Fire hazards and infectious diseases result in great threats to public safety and human health, thus developing multi-functional materials to deal with these issues is critical and yet has remained challenging to date. In this work, we report a facile and eco-friendly synthetic approach for the preparation of calcium alginate/silver chloride (CA/AgCl) nanocomposites with dual functions of excellent flame-retardant and antibacterial activity. Multi characterization techniques and antibacterial assays were performed to investigate the flame-retardant and antibacterial properties of the CA/AgCl nanocomposites. The obtained results show that the CA/AgCl nanocomposites exhibited much higher limiting oxygen index value (> 60%) than that of CA (48%) with a UL-94 rating of V-0. Moreover, CA/AgCl particularly displayed an efficiently smoke-suppressive feature by achieving a total smoke release value of 2.7 m/m, which was reduced by 91%, compared to CA. The antibacterial rates of the CA/AgCl nanocomposites against E. coli and S. aureus were measured to be 99.67% and 99.77%, respectively, while CA showed quite weak antibacterial rates. In addition, the flame-retardant and antibacterial mechanisms were analyzed and proposed based on the experimental data. This study provides a novel nanocomposite material with both flame-retardant and antibacterial properties which show promising application prospects in the fields of decorative materials and textile industry.
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http://dx.doi.org/10.1007/s10570-021-03825-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982765PMC
March 2021

Toward reducing thrombus recurrence rate in management of patients with confirmed left ventricular thrombi.

ESC Heart Fail 2021 06 21;8(3):2359-2360. Epub 2021 Mar 21.

Department of Cardiology, The Key Laboratory of Cardiovascular Disease of Wenzhou, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China.

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http://dx.doi.org/10.1002/ehf2.13319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120361PMC
June 2021

State of the art in parallel ankle rehabilitation robot: a systematic review.

J Neuroeng Rehabil 2021 03 20;18(1):52. Epub 2021 Mar 20.

Beijing Key Laboratory of Advanced Manufacturing Technology, Faculty of Materials and Manufacturing, Beijing University of Technology, No.100, Pingleyuan, Chaoyang District, Beijing, 100124, China.

Background: The ankle joint complex (AJC) is of fundamental importance for balance, support, and propulsion. However, it is particularly susceptible to musculoskeletal and neurological injuries, especially neurological injuries such as drop foot following stroke. An important factor in ankle dysfunction is damage to the central nervous system (CNS). Correspondingly, the fundamental goal of rehabilitation training is to stimulate the reorganization and compensation of the CNS, and to promote the recovery of the motor system's motor perception function. Therefore, an increasing number of ankle rehabilitation robots have been developed to provide long-term accurate and uniform rehabilitation training of the AJC, among which the parallel ankle rehabilitation robot (PARR) is the most studied. The aim of this study is to provide a systematic review of the state of the art in PARR technology, with consideration of the mechanism configurations, actuator types with different trajectory tracking control techniques, and rehabilitation training methods, thus facilitating the development of new and improved PARRs as a next step towards obtaining clinical proof of their rehabilitation benefits.

Methods: A literature search was conducted on PubMed, Scopus, IEEE Xplore, and Web of Science for articles related to the design and improvement of PARRs for ankle rehabilitation from each site's respective inception from January 1999 to September 2020 using the keywords " parallel", " ankle", and " robot". Appropriate syntax using Boolean operators and wildcard symbols was utilized for each database to include a wider range of articles that may have used alternate spellings or synonyms, and the references listed in relevant publications were further screened according to the inclusion criteria and exclusion criteria.

Results And Discussion: Ultimately, 65 articles representing 16 unique PARRs were selected for review, all of which have developed the prototypes with experiments designed to verify their usability and feasibility. From the comparison among these PARRs, we found that there are three main considerations for the mechanical design and mechanism optimization of PARRs, the choice of two actuator types including pneumatic and electrically driven control, the covering of the AJC's motion space, and the optimization of the kinematic design, actuation design and structural design. The trajectory tracking accuracy and interactive control performance also need to be guaranteed to improve the effect of rehabilitation training and stimulate a patient's active participation. In addition, the parameters of the reviewed 16 PARRs are summarized in detail with their differences compared by using figures and tables in the order they appeared, showing their differences in the two main actuator types, four exercise modes, fifteen control strategies, etc., which revealed the future research trends related to the improvement of the PARRs.

Conclusion: The selected studies showed the rapid development of PARRs in terms of their mechanical designs, control strategies, and rehabilitation training methods over the last two decades. However, the existing PARRs all have their own pros and cons, and few of the developed devices have been subjected to clinical trials. Designing a PARR with three degrees of freedom (DOFs) and whereby the mechanism's rotation center coincides with the AJC rotation center is of vital importance in the mechanism design and optimization of PARRs. In addition, the design of actuators combining the advantages of the pneumatic-driven and electrically driven ones, as well as some new other actuators, will be a research hotspot for the development of PARRs. For the control strategy, compliance control with variable parameters should be further studied, with sEMG signal included to improve the real-time performance. Multimode rehabilitation training methods with multimodal motion intention recognition, real-time online detection and evaluation system should also be further developed to meet the needs of different ankle disability and rehabilitation stages. In addition, the clinical trials are in urgent need to help the PARRs be implementable as an intervention in clinical practice.
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http://dx.doi.org/10.1186/s12984-021-00845-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981854PMC
March 2021

Modulation of the ERK1/2-MMP-2 pathway in the sclera of guinea pigs following induction of myopia by flickering light.

Exp Ther Med 2021 Apr 19;21(4):371. Epub 2021 Feb 19.

Department of Ophthalmology, Jinshan Hospital of Fudan University, Shanghai 201508, P.R. China.

It has been shown that flickering light can affect the development of eyeballs. However, the exact mechanism remains unclear. The ERK1/2-MMP-2 pathway is a classic pathway involved in the modulation of the extracellular matrix (ECM) in cancer tissues. However, to the best of our knowledge, the role of this pathway in modulating the scleral ECM in myopia has not been previously examined. The present study aimed to determine the effects of the ERK1/2-MMP-2 pathway on the formation of flickering light-induced myopia (FLM). Guinea pigs were raised under illumination at a flash rate of 0.5 Hz for 6 weeks to induce FLM. Peribulbar injections of dimethylsulfoxide or PD98059 (an inhibitor of phospho-ERK1/2) were administered starting at the third week of FLM modeling. Refraction was measured prior to and following treatments. The thickness of the posterior sclera (PS) was measured under a light microscope following H&E staining. The mRNA levels of MMP-2 were detected by the reverse transcription-quantitative PCR assay. The expression levels of MMP-2 and ERK1/2 were assayed by western blot and immunohistochemical analyses. Following 6 weeks of treatment, the refraction of the FLM group became more myopic compared with that of the control group, while PD98059 treatment inhibited the changes noted in the refraction. A marked reduction in the thickness of PS was observed in the FLM group, while PD98059 inhibited the remodeling of PS. In addition, the expression levels of MMP-2 and protein levels of phospho-ERK1/2 were increased in the FLM group, while PD98059 significantly inhibited MMP-2 mRNA and protein levels. These results indicated that ERK1/2-MMP-2 may be involved in the formation of FLM in guinea pigs by regulating the remodeling of PS.
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http://dx.doi.org/10.3892/etm.2021.9802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903414PMC
April 2021

Circular RNA circAFF2 accelerates gastric cancer development by activating miR-6894-5p and regulating ANTXR 1 expression.

Clin Res Hepatol Gastroenterol 2021 May 12;45(3):101671. Epub 2021 Mar 12.

Department of Respiratory medicine, the Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, China. Electronic address:

Background: Circular RNAs (circRNAs) contain a new class of non-coding RNAs that play an important role in adjusting biological function and gene expression. But the function of circRNAs in gastric cancer remains unclear. In the present research, we explored the functions of circular RNA AFF2(circAFF2, hsa_circ_0001947) in gastric cancer cells and an animal model of gastric cancer.

Methods: The expression of circAFF2, microRNA-6894-5p (miR-6894-5p), and Anthrax toxin receptor 1 (ANTXR 1) were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cell counting kit 8 (CCK-8) and transwell assays were used to analyze the knockdown effects of circAFF2, miR-6894-5p, and overexpression of ANTXR 1 on cell proliferation, migration, and invasion abilities. Binding interactions between, circAFF2 and miR-6894-5p and between, miR-6894-5p and ANTXR 1 were detected by Dual-luciferase reporter assays. Levels of protein expression were analyzed by Western blotting. Tumor models were established by subcutaneous injection of tumor cells in nude mice.

Result: The result showed that circAFF2 expression was significantly increased in gastric cancer cell lines and tissues. The knockdown of circAFF2 dramatically suppressed the cell migration, invasion and proliferation of gastric cancer cells. In vivo studies showed that knockdown of circAFF2 delayed tumor growth. Furthermore, we revealed that circAFF2 functioned as a sponge to absorb miR-6984-5p and elevated the expression of ANTXR 1.

Conclusion: CircAFF2 acts as an oncogene in gastric cancer and exerts its effects via miR-6894-5p/ANTXR 1 signaling.
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http://dx.doi.org/10.1016/j.clinre.2021.101671DOI Listing
May 2021

Colorimetric biosensors for point-of-care virus detections.

Mater Sci Energy Technol 2020 23;3:237-249. Epub 2019 Oct 23.

Institute of Materials Research and Engineering, ASTAR (Agency for Science, Technology and Research), 2 Fusionopolis Way, #08-03, Innovis, Singapore 138634, Singapore.

Colorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.
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http://dx.doi.org/10.1016/j.mset.2019.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148662PMC
October 2019

Clinical characteristics and outcomes in patients with echocardiographic left ventricular spontaneous echo contrast.

Int J Cardiol 2021 05 10;330:245-250. Epub 2021 Feb 10.

Department of Cardiology, The Key Laboratory of Cardiovascular Disease of Wenzhou, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China; Department of Cardiology, Longgang City People's Hospital, Longgang, Zhejiang 325802, PR China. Electronic address:

Background: Spontaneous echo contrast (SEC) is a known precursor to thrombus formation and thromboembolic events. This study aims to demonstrate the clinical characteristics and outcomes of patients with left ventricular spontaneous echo contrast (LV-SEC).

Methods: Patients with consecutive echocardiogram performed from October 2009 to September 2019 were enrolled in this retrospective, single-center study. Those with LV-SEC were included, while patients complicated by left ventricular thrombus, with history of infective endocarditis, prosthetic valves, or lost to follow-up were excluded. The clinical endpoint was 1-year thromboembolic events (i.e. stroke and peripheral embolism).

Results: Among 417 patients (mean age 63.5 ± 14.7 years; 86.8% men) with LV-SEC, the incidence of 1-year embolism was 12.9%. In multivariate Cox proportional hazard model, significant risk factors for thromboembolic event were age [hazard ratio (HR) = 1.022, 95% confidence interval (CI): 1.000-1.045], atrial fibrillation (AF) (HR = 2.292, 95% CI: 1.237-4.244), hemoglobin (HR = 1.032, 95% CI: 1.017-1.047), left ventricular ejection fraction (LVEF) (HR = 1.021, 95% CI: 1.002-1.041), and anticoagulant therapy (HR = 0.310, 95% CI: 0.168-0.572). For patients with repeated measurements for echocardiography, D-dimer (HR = 1.137, 95% CI: 1.051-1.231), and LVEF (HR = 0.961, 95% CI: 0.928-0.996) were independently associated with the persistent LV-SEC.

Conclusion: The present study reported a high incidence of 1-year thromboembolic event in patients with LV-SEC. Age, AF, hemoglobin, LVEF were independent risk factors for 1-year embolism and a reduced risk of embolism was observed among patients with anticoagulation therapy. Additionally, D-dimer and LVEF are independently associated with the persistent LV-SEC.
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http://dx.doi.org/10.1016/j.ijcard.2021.02.005DOI Listing
May 2021

A novel risk model to predict first-ever ischemic stroke in heart failure with reduced ejection fraction.

Aging (Albany NY) 2021 02 1;13(4):5332-5341. Epub 2021 Feb 1.

Department of Cardiology, The Key Laboratory of Cardiovascular Disease of Wenzhou, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang, P.R. China.

Patients with heart failure are at increased risk for ischemic stroke. We aim to develop a more accurate stroke risk prediction tools identify high-risk patients with heart failure with reduced ejection fraction (HFrEF). Patient data were extracted retrospectively from the electronic medical database between January 2009 and February 2019. Univariate and multivariate Cox regression analysis were performed to identify independent predictors, which were utilized to construct a nomogram for predicting ischemic stroke. AUROC analysis was used to compare the prognostic value between the new risk score and CHADS/CHADS-VASc scores. In 6087 patients with HFrEF, the risk of first-ever ischemic stroke was 5.8% events/pts-years (n=468) during 8007.2 person-years follow-up. A nomogram constructed by integrating 6 variables, including age, atrial fibrillation (AF), deep vein thrombosis (DVT), d-dimer, anticoagulant use and spontaneous echocardiographic contrast (SEC)/left ventricular thrombus (LVT), exhibited a greater area under the curve of 0.727, 0.728 and 0.714 than that by CHADS score (0.515, 0.522 and 0.540), and by CHADS-VASc score (0.547, 0.553 and 0.562) for predicting first-ever ischemic stroke at hospitalization, 30-day and 6-month follow-up (all p<0.001). This novel stroke risk score performed better than existing CHADS/ CHADS-VASc scores and showed improvement in predicting first-ever ischemic stroke in HFrEF patients.
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http://dx.doi.org/10.18632/aging.202458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950228PMC
February 2021

The prevalence, predictors, and outcomes of spontaneous echocardiographic contrast or left ventricular thrombus in patients with HFrEF.

ESC Heart Fail 2021 04 25;8(2):1284-1294. Epub 2021 Jan 25.

Department of Cardiology, The Key Laboratory of Cardiovascular Disease of Wenzhou, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Wenzhou, Zhejiang, 325000, China.

Aims: This study aimed to determine prevalence, predictors, and association with ischaemic stroke risk of spontaneous echocardiographic contrast (SEC) or left ventricular thrombus (LVT) in patients with heart failure with reduced ejection fraction (HFrEF).

Methods And Results: Clinical, echocardiographic, and follow-up data from January 2009 through February 2019 were retrospectively extracted from electronic medical records of patients with heart failure with left ventricular ejection fraction < 40% by echocardiography on admission, with follow-up to February 2020. Of 9485 consecutive patients with HFrEF, 123 (1.3%) presented LVT and 331 (3.5%) presented SEC. Patients with vs. those without SEC/LVT had larger left ventricular end-diastolic volume (199.5 ± 77.7 vs. 165.8 ± 61.3 mL, P < 0.001), lower left ventricular ejection fractions (29.5 ± 7.0% vs. 33.7 ± 5.5%, P < 0.001), and more often ischaemic cardiomyopathy, apical aneurysm, chronic kidney diseases, and smoking habit. In Cox regression analysis, SEC and LVT were independent predictors for ischaemic stroke occurrence [hazard ratio (HR) = 2.40, 95% confidence interval (CI): 1.74-3.31; HR = 4.52, 95% CI: 2.77-7.40, both P < 0.001]. In patients with those without SEC or LVT, stroke risk was higher among those not on anticoagulants (HR = 2.55, 95% CI: 1.85-3.53; HR = 4.71, 95% CI: 2.84-7.81, both P < 0.001), but similar among those on anticoagulants (P > 0.05). In patients with sinus rhythm, the associations between SEC/LVT and ischaemic stroke persist with HRs of 2.57 (95% CI: 1.69-3.92) and 5.74 (95% CI: 3.38-9.75).

Conclusions: In patients with HFrEF, SEC was not uncommon and increased risk of ischaemic stroke as well as LVT. Anticoagulants could play a role in the reduction of stroke risk, suggesting that patients with SEC/LVT, even those in sinus rhythm, would benefit from systemic anticoagulation treatment.
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http://dx.doi.org/10.1002/ehf2.13196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006613PMC
April 2021

Association between Helicobacter pylori infection and irritable bowel syndrome: A systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(50):e22975

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Nanchang University.

Background: In recent years, the incidence of IBS has gradually increased, and it is considered as one of the most common functional gastrointestinal diseases. However, the etiology of IBS is still unclear, and expectations are rising for more targeted treatments. Many clinical trials have explored the link between Helicobacter pylori (H pylori) and IBS, with different conclusions. Therefore, we conducted a meta-analysis to explore whether there is an association between H pylori and IBS, which is of great significance for targeted treatment of IBS.

Methods: We performed a systematic review and meta-analysis of the association between H pylori and IBS. We searched PubMed, EMBASE, Medline and the Cochrane Library to collect related studies. OR was used to describe the ratio of the probability of the H pylori infection occurring in IBS patients versus the controls. Heterogeneity was assessed by subgroup and meta-regression analysis.

Results: Eight studies, including 1861 patients, assessed the association between H pylori infection and IBS. The OR of H pylori in IBS patients compared to controls was 1.32 (95% CI: 0.94-1.87; P = 0.11). Subgroup analyses showed a difference between IBS patients diagnosed with Roman III criteria and those diagnosed with non-Roman III criteria.

Conclusions: Our study suggests that H pylori may have a positive effect on the development of IBS. Although the differences were not statistically significant, there were significant differences among subgroups of patients. Considering the limitations and heterogeneity, high quality studies are needed to further explore the effect of H pylori on the development of IBS.
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http://dx.doi.org/10.1097/MD.0000000000022975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738067PMC
December 2020

Time trend of axial length and associated factors in 4- and 5-year-old children in Shanghai from 2013 to 2019.

Int Ophthalmol 2021 Mar 12;41(3):835-843. Epub 2020 Nov 12.

Department of Ophthalmology, Jinshan Hospital of Fudan University, 1508 Longhang Road, Shanghai, 201508, China.

Purpose: To evaluate the time trend of axial length (AL) and associated factors in 4- and 5-year-old children in Shanghai from 2013 to 2019.

Methods: This was a 7-year observational study of 985 four-year-old and 1059 five-year-old children in Shanghai. AL, horizontal and vertical corneal curvature, spherical equivalent (SE), and body height and weight were measured. Furthermore, a questionnaire was collected, including time outdoors and bad eyesight habits.

Results: In 4-year-old children, no significant difference was found in AL (P = 0.526), but significant differences were observed in SE (P = 0.001), horizontal corneal curvature (P = 0.006), vertical corneal curvature (P = 0.004), height (P < 0.001), and weight (P = 0.022) from 2013 to 2019. In 5-year-old children, no significant differences were found in AL (P = 0.304), SE (P = 0.200), or weight (P = 0.292), but significant differences were observed in horizontal corneal curvature (P = 0.040), vertical corneal curvature (P = 0.015), and height (P < 0.001) from 2013 to 2019. Multivariate analyses revealed that AL was mainly significantly associated with boys and time outdoors in the 4- and 5-year-old children.

Conclusions: The AL of 4- and 5-year-old children remained relatively stable in Shanghai from 2013 to 2019. Longitudinal studies are needed to confirm the relationship between AL elongation and environmental risk factors.
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http://dx.doi.org/10.1007/s10792-020-01637-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943426PMC
March 2021

Exome-Wide Association Analysis Suggests LRP2BP as a Susceptibility Gene for Endothelial Injury in Systemic Sclerosis in the Han Chinese Population.

J Invest Dermatol 2021 May 15;141(5):1254-1263.e6. Epub 2020 Oct 15.

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China.

Genetic factors play a key role in the pathogenesis of autoimmune diseases, whereas the disease-causing variants remain largely unknown. Herein, we performed an exome-wide association study of systemic sclerosis in a Han Chinese population. In the discovery stage, 527 patients with systemic sclerosis and 5,024 controls were recruited and genotyped. In the validation study, an independent sample set of 479 patients and 1,096 controls were examined. In total, we found that four independent signals reached genome-wide significance. Among them, rs7574865 (P = 3.87 × 10) located within signal transducer and activator of transcription 4 gene was identified previously using samples of European ancestry. Additionally, another signal including three SNPs in linkage disequilibrium might be unreported susceptibility loci located in the epidermis differentiation complex region. Furthermore, two SNPs located within exon 3 of IGHM (rs45471499, P = 1.15 × 10) and upstream of LRP2BP (rs4317244, P = 4.17 × 10) were found. Moreover, rs4317244 was identified as an expression quantitative trait locus for LRP2BP that regulates tight junctions, cell cycle, and apoptosis in endothelial cell lines. Collectively, our results revealed three signals associated with systemic sclerosis in Han Chinese and suggested the importance of LRP2BP in systemic sclerosis pathogenesis. Given the limited sample size and discrepancies between previous results and our study, further studies in multiethnic populations are required for verification.
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http://dx.doi.org/10.1016/j.jid.2020.07.039DOI Listing
May 2021

Cannulation procedure optimization for patients with duodenal papillary tumors.

Surg Endosc 2021 Oct 9;35(10):5635-5642. Epub 2020 Oct 9.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, 17 Yongwai Zheng Street, Nanchang, 330006, Jiangxi, China.

Background: The goal of this study was to compare the efficacy and safety of needle-knife fistulotomy (NKF) to that of conventional cannulation methods (CCMs) when used for primary biliary access in patients with duodenal papillary tumors.

Methods: Consecutive patients who had duodenal papillary tumors and who underwent endoscopic retrograde cholangiopancreatography (ERCP) were retrospectively enrolled. Successful cannulation rates, cannulation and procedure times, and the prevalence of adverse events were compared between the NKF and CCM groups.

Results: A total of 404 patients (NKF, n = 124; CCM, n = 280) with duodenal papillary tumors were included. The primary and overall cannulation rates were 92.1% (372/404) and 96.0% (388/404), respectively. Compared to CCMs, NKF was associated with a significantly higher successful cannulation rate (99.2% versus 88.9%, P < 0.001) and significantly lower cannulation times (2.1 ± 2.0 min versus 4.7 ± 5.2 min), procedure times (8.8 ± 3.8 min versus 12.9 ± 7.6 min), and unintentional pancreatic duct cannulation rates (1.6% versus 20%), with P < 0.001 for all. Overall adverse events occurred less frequently in the NKF group (3.2% versus 10.7%, P = 0.011). Of these adverse events, post-ERCP pancreatitis (PEP) was significantly lower in the NKF group than in the CCM group (1.6% versus 6.8%, P = 0.03). Bleeding and cholangitis rarely occurred with either cannulation method (0.8% versus 2.1%, P = 0.681, and 0.8% versus 1.7%, P = 0.671, respectively).

Conclusion: NKF is a more effective and safer procedure than CCMs for patients with duodenal papillary tumors.
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http://dx.doi.org/10.1007/s00464-020-08076-4DOI Listing
October 2021

Discovery of Real-Space Topological Ferroelectricity in Metallic Transition Metal Phosphides.

Adv Mater 2020 Nov 7;32(46):e2003479. Epub 2020 Oct 7.

Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich GmbH, Jülich, 52425, Germany.

Ferroelectric metals-with coexisting ferroelectricity and structural asymmetry-challenge traditional perceptions because free electrons screen electrostatic forces between ions, the driving force of breaking the spatial inversion symmetry. Despite ferroelectric metals having been unveiled one after another, topologically switchable polar objects with metallicity have never been identified so far. Here, the discovery of real-space topological ferroelectricity in metallic and non-centrosymmetric Ni P is reported. Protected by the rotation-inversion symmetry operation, it is found that the balanced polarity of alternately stacked polyhedra couples intimately with elemental valence states, which are verified using quantitative electron energy-loss spectroscopy. First-principles calculations reveal that an applied in-plane compressive strain creates a tunable bilinear double-well potential and reverses the polyhedral polarity on a unit-cell scale. The dual roles of nickel cations, including polar displacement inside polyhedral cages and a 3D bonding network, facilitate the coexistence of topological polarity with metallicity. In addition, the switchable in-plane polyhedral polarity gives rise to a spin-orbit-coupling-induced spin texture with large momentum-dependent spin splitting. These findings point out a new direction for exploring valence-polarity-spin correlative interactions via topological ferroelectricity in metallic systems with structural asymmetry.
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http://dx.doi.org/10.1002/adma.202003479DOI Listing
November 2020

Efficacy and safety of ERCP in patients with gastroesophageal varices.

Medicine (Baltimore) 2020 Sep;99(37):e22051

Departments of Gastroenterology.

Addressing pancreaticobiliary disorders concomitant with gastroesophageal varices remains challenging. The goal of this study was to evaluate and compare the efficacy and safety of endoscopic retrograde cholangiopancreatography (ERCP) in cirrhotic and noncirrhotic patients with gastroesophageal varices.We retrospectively analyzed the data of consecutive patients with gastroesophageal varices who underwent ERCP.Two hundred seventy ERCP procedures were performed on 208 patients. The overall technical success rate was 98.5%, and no difference was found between cirrhotic and noncirrhotic patients (98.7% vs 97.7%, P = .511); of these, endoscopic retrograde biliary drainage, endoscopic metal biliary endoprosthesis placement, endoscopic retrograde pancreatic drainage, and stone extraction were conducted in 173/270 (64.1%), 27/270 (10.0%), 26/270 (9.6%), and 116/270 (43.0%) cases, respectively. Endoscopic retrograde biliary drainage and stone extraction were more frequently performed in cirrhotic cases (67.7% versus 45.5%, P = .005; 46.5% versus 25.0%, P = .009, respectively), while the noncirrhotic group had significantly higher rates of endoscopic metal biliary endoprosthesis placement (31.8% versus 5.8%, P = .000) and endoscopic retrograde pancreatic drainage (18.2% versus 8.0%, P = .036) than the cirrhotic group. The overall rate of adverse events was 21.1%, including fever (6.7%), post-ERCP pancreatitis ( 3.0%), hyperamylasemia (6.3%), duodenal papilla bleeding (3.3%), cardiac mucosal laceration (1.1%), and perforation (0.4%). No differences in any of the adverse events were found between the 2 groups. Additionally, gastroesophageal variceal bleeding occurred in 1 patient with grade III varices 7 days after ERCP.ERCP may be effective and safe for patients with gastroesophageal varices, irrespective of the etiologies caused by liver cirrhosis.
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http://dx.doi.org/10.1097/MD.0000000000022051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489748PMC
September 2020

Clinicopathological and genomic features of breast mucinous carcinoma.

Breast 2020 Oct 6;53:130-137. Epub 2020 Aug 6.

Department of General Surgery, Yuyao People's Hospital of Zhejiang Province, Yuyao, Zhejiang, 315400, PR China. Electronic address:

Introduction: Mucinous carcinoma (MC) of the breast is a special histological type of breast cancer. Clinicopathological characteristics and genomic features of MC is not fully understood.

Materials And Methods: 186,497 primary breast cancer patients from SEER database diagnosed with invasive ductal carcinoma (IDC) or MC were included. 801 primary IDC or MC patients from TCGA cohort were included for transcriptomic and genomic analysis.

Results: MC patients were older, had lower tumor grade and T and N stage, higher hormone receptor positive proportions and lower HER2 positive proportions than IDC patients. Kaplan-Meier plots showed that the breast cancer-specific survival (BCSS) of MC patients was significantly better than IDC patients (P < 0.001). However, after adjusting for clinicopathological factors, survival advantage of MC disappeared. In terms of genomic features of MC, representative upregulated genes of MC in transcriptomic level were MUC2, TFF1 and CARTPT. Upregulated pathways of MC included neurotransmitter-related pathways. Moreover, MC was featured by the amplification of 6p25.2, 6q12 and 11q12.3.

Conclusion: MC is a distinct histological subtype compared with IDC in terms of clinicopathological characteristics and genomic features. Further investigation need to be conducted to explore the formation of this specific histological subtype.
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http://dx.doi.org/10.1016/j.breast.2020.07.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7419658PMC
October 2020

Correction to: The IgG galactosylation ratio is higher in spondyloarthritis patients and associated with the MRI score.

Clin Rheumatol 2020 10;39(10):3159

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai, China.

In the original version of the above article the References 19 and 20 were incorrect which cannot describe the development of the SPARCC score.
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http://dx.doi.org/10.1007/s10067-020-05314-xDOI Listing
October 2020

Association between vein diameters, reflux characteristics, and clinical severity in patients with chronic venous insufficiency in Northwest China.

J Vasc Surg Venous Lymphat Disord 2021 03 28;9(2):401-408.e1. Epub 2020 Jul 28.

University of California, Davis, Vascular Center, Sacramento, Calif. Electronic address:

Objective: Our goal was to summarize the relationship between vein diameters, reflux characteristics, and clinical severity in consecutive patients with chronic venous insufficiency (CVI) in Northwest China.

Methods: We evaluated 531 consecutive patients with CVI (249 women) who presented to the Department of Ultrasound of Xijing Hospital from September 2017 to July 2019. Reflux times and the mean diameters of the great saphenous, the small saphenous, and the calf perforator veins based on duplex ultrasound scans obtained in the standing position were recorded. Venous-specific assessment tools-the Heaviness, Achiness, Swelling, Throbbing, Itching (HASTI) score, the Venous Clinical Severity Score (VCSS), and the Clinical, Etiological, Anatomical, Pathophysiological (CEAP) class-were analyzed. Regression analysis was used to investigate the relationship between the clinical scores, vein diameters, and reflux times. A P value of less than .05 was considered statistically significant.

Results: We analyzed 531 consecutive patients with 728 limbs. The mean age was 55.24 ± 11.38 years; the mean body mass index (BMI) was 24.75 ± 3.49 kg/m. Three hundred thirty-four patients (62.9%) presented with unilateral limb findings and 197 (37.1%), with bilateral limb involvement. No significant changes were noted in age and BMI across CEAP classes (F = 2.322 and F = 3.917, respectively; P > .05 for both). Both the HASTI score (r = 0.8741; P < .001) and the VCSS (r = 0.9257; P < .001) correlated with the CEAP class. The HASTI score strongly correlated with the mean diameters of the great saphenous and small saphenous veins (r = 0.9252, r = 0.6304, respectively; P < .001 for both) similarly to VCSS (r = 0.9396, r = 0.7195, respectively; P < .001 for both). The HASTI score and VCSS correlated equally with the mean diameters of the calf perforator veins (r = 0.7773 and r = 0.7781, respectively; P < .001 for both). In those with C6, both great saphenous vein (F = 4.608; P < .001) and small saphenous vein reflux times (F = 14.97; P < .001) were significantly higher than those in C1. Both the HASTI score and VCSS strongly associated with the reflux times of the great saphenous (r = 0.7706 and r = 0.8181, respectively; P < .001 for both) and small saphenous veins (r = 0.6470 and r = 0.7865, respectively; P < .001 for both).

Conclusions: This analysis is one of the few epidemiologic studies of patients with CVI in Northwest China. Age and BMI did not correlate with CEAP class. Both the HASTI score and VCSS correlated strongly with the CEAP classification; vein diameters and reflux time in both the great saphenous vein and the small saphenous vein, indicating the validity of these outcome tools to venous hemodynamics and to CVI in general.
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http://dx.doi.org/10.1016/j.jvsv.2020.07.006DOI Listing
March 2021

Morin attenuates STZ-induced diabetic retinopathy in experimental animals.

Saudi J Biol Sci 2020 Aug 8;27(8):2139-2142. Epub 2020 Jun 8.

Department of Ophthalmology, Jinshan Hospital of Fudan University, Jinshan District, Shanghai 201508, China.

Diabetic retinopathy (DR) occurs in untreated diabetic patients due to the strong influence of oxidative stress. Bioflavonoids are well known for their antioxidant property. Morin, a bioflavonoid, has been demonstrated for its antioxidant as well as antidiabetic activity. Thus, this research work intended to determine the ameliorative impact of morin in DR rats using STZ-induced type 1 diabetic model. To induce type 1 diabetic in rats STZ (60 mg/kg) was administered intraperitoneally. Grouping of animals was done as described below (n = 6), where, group I - normal control, group II - diabetic control, group III - morin (25 mg/kg), group IV - morin (50 mg/kg), and group V - metformin (350 mg/kg) were used. All the animals underwent treatment for 60 days as given above. It was observed that supplementation of morin (25 and 50 mg/kg) showed a noteworthy decline in elevated serum glucose level. Moreover, decrease in the level of LPO and improved activity of endogenous antioxidants (GPx, CAT, and SOD) was observed in morin treated groups. It also notably drops the concentration of TNF-α, IL-1β, and VEGF in the tissue homogenate of the retina. Furthermore, it increased the retinal thickness and cell count in the ganglion cell layer of the retina in diabetic animals. Hence, we can conclude that morin encumbers the progression of DR in diabetic animals, which may be via antioxidant property and suppression of TNF-α, IL-1β, and VEGF.
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http://dx.doi.org/10.1016/j.sjbs.2020.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7376113PMC
August 2020

FeO Nanoparticles Supported on Modified Coal toward Catalytic Hydrogenation of Coal to Oil.

ACS Omega 2020 Jul 1;5(27):16789-16795. Epub 2020 Jul 1.

School of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, Xinjiang, China.

Iron-supported catalysts exhibit good catalytic performance in direct coal liquefaction (DCL), but the effect of the carrier on the performance of the composite catalyst is unclear. In this paper, a simple solid-state synthesis strategy for the preparation of coal-loaded FeO nanoparticles (FeO/Coal) and the hydrochloric acid treatment of coal-loaded FeO nanoparticles (FeO/Coal-HCl) is presented. The resulting composite was used as a catalyst for DCL. The effects of supports on the structure and performance of iron-supported catalysts have been illustrated. After the acid pretreatment of the catalyst carrier coal, the surface structure and functional groups changed, which affected the aggregation morphology of the FeO active component. The FeO/Coal-HCl catalyst improved the catalytic performance of DCL with conversion and oil yield of 98.02 and 49.96 wt %, respectively. The result shows that pretreatment can be an effective way to modify the surface of the catalyst carrier coals, thereby further improving the catalytic performance of composites. The iron-based composites prepared by the solid-state route show great potential as supported catalysts in DCL.
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http://dx.doi.org/10.1021/acsomega.0c01836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364719PMC
July 2020

Loss of epithelial AR increase castration resistant stem-like prostate cancer cells and promotes cancer metastasis via TGF-β1/EMT pathway.

Transl Androl Urol 2020 Jun;9(3):1013-1027

Department of Urology, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China.

Background: Previous study has reported that loss of epithelial androgen receptor (AR) may promote tumor progression and cause TRAMP mouse model die earlier. The detail mechanisms, however, remain unclear.

Methods: Immunohistochemistry assay, Western blot and real-time PCR were used to detect the expression of epithelial and mesenchymal markers. RNA extraction, RT-PCR, quantitative RT-PCR, BrdU incorporation assays, flow cytometry and other experimental technics were also used in present work.

Results: Decreased expression of epithelial markers (Cytokeratin 8, NKX3.1 and E-cadherin) and increased expression of mesenchymal markers (α-SMA, Vimentin, and N-cadherin) in were found in AR knockout TRAMP tumors. Further investigation indicated that AR signal deprivation is associated with cell morphology transition, high cell mobility, high cell invasion rate and resistance to anoikis in TRAMP prostate tumor cells. Together, these findings implied knockout AR in TRAMP prostate tumor may lead to EMT, which may result in earlier metastasis, and then cause TRAMP mice die earlier. TGF-β1 is responsible for EMT in AR knockout TRAMP tumor cells.

Conclusions: In conclusion, ADT therapy induced hormone refractory prostate cancer may gain the ability of metastasis through cell's EMT which is a phase of poor differentiation. Anti-EMT drugs should be developed to battle the tumor metastasis induced by ADT therapy.
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http://dx.doi.org/10.21037/tau.2020.03.02DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354287PMC
June 2020
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