Publications by authors named "Xiaodong Ma"

310 Publications

Characterization of the complete chloroplast genome of (Lour.) Schott.

Mitochondrial DNA B Resour 2021 Mar 18;6(3):1018-1019. Epub 2021 Mar 18.

College of Medicine, Pingdingshan University, Pingdingshan City, Henan Province, China.

(Lour.) Schott () is a traditional Chinese medicine. However, the chloroplast genome has not been reported. Here, we assembled and analyzed the complete chloroplast (CP) genome of We found that the genome of is 165,398 bp in length and contains a large single-copy (LSC) region of 92,861 bp, a small single-copy (SSC) region of 20,943 bp and an inverted repeat (IR) region of 25,797 bp. The genome contains 130 genes including 85 protein-coding genes, 8 rRNA and 37 tRNA. Phylogenetic analysis indicated that is close to This study provides useful data for the development of molecular markers and identification of
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http://dx.doi.org/10.1080/23802359.2021.1895690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995840PMC
March 2021

An integrated migration and transformation model to evaluate the occurrence characteristics and environmental risks of Nitrogen and phosphorus in constructed wetland.

Chemosphere 2021 Mar 13;277:130219. Epub 2021 Mar 13.

Tianjin Lingang Construction Development Co., Ltd, Tianjin, 300450, China.

In this study, an integrated migration and transformation (IMT) model based on microbial action, plant absorption, sediment release and substrate adsorption was firstly established to evaluate the temporal-spatial distribution of N and P in Lingang hybrid constructed wetland (CW), Tianjin. Compared to the conventional transformation model that only considers the microbial action, the IMT model could accurately predict the occurrence characteristics of N and P. In Lingang CW, NO-N (0.56-3.63 mg/L) was the most important form of N, and the TP was at a relatively low concentration level (0.04-0.07 mg/L). The spatial distribution results showed that a certain amount of N and P could be removed by CW. Form the temporal perspective, the N and P concentrations were greatly affected by the dissolved oxygen (DO). The simulated values obtained by IMT model indicated that the distribution of N and P was more affected by the temporality compared with the spatiality, which was consistent with measured values. Besides, the PCA indicated that TN, NO-N and DO were important factors, which affected the water quality of CW. The Nemerow pollution index method based on the simulated values indicated that Lingang CW was overall moderately polluted, and the subsurface area was the main functional unit of pollutants removal in CW. This work provides a new model for accurately predicting the occurrence characteristics of N and P pollutants in CW, which is of great significance for identifying its environmental risks and optimizing the construction of wetlands.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130219DOI Listing
March 2021

The Meaning of Temporal Balance: Does Meaning in Life Mediate the Relationship Between a Balanced Time Perspective and Mental Health?

Eur J Psychol 2021 Feb 26;17(1):119-133. Epub 2021 Feb 26.

Department of Psychology, University of Houston - Clear Lake, Houston, TX, United States.

The construct of a Balanced Time Perspective (BTP) predicts a variety of indices of mental health and well-being. We argue that one possible intermediate link between BTP and well-being may be an individual's sense of presence of meaning in life. Participants ranging in age from 19 to 88 years (N = 192) completed two measures of time perspective (Zimbardo Time Perspective Inventory [ZTPI] and the modified Balanced Time Perspective Scale [mBTPS]), mental health, personality, and meaning in life. Correlational results showed that a BTP, mental health, and meaning in life were positively interrelated. Hierarchical regression models showed that a BTP (as measured with the mBTPS) explained additional variance in mental health beyond demographic, personality, and ZTPI scores. Mediation analyses showed that meaning served as a significant indirect link between BTP and well-being.
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http://dx.doi.org/10.5964/ejop.2415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957847PMC
February 2021

Detection of the SARS-CoV-2 D614G mutation using engineered Cas12a guide RNA.

Biotechnol J 2021 Feb 17:e2100040. Epub 2021 Feb 17.

Guangzhou Laboratory, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Detection of pathogens with single-nucleotide variations is indispensable for the disease tracing, but remains technically challenging. The D614G mutation in the SARS-CoV-2 spike protein is known to markedly enhance viral infectivity but is difficult to detect. Here, we report an effective approach called "synthetic mismatch integrated crRNA guided Cas12a detection" (symRNA-Cas12a) to detect the D614 and G614 variants effectively. Using this method, we systemically screened a pool of crRNAs that contain all the possible nucleotide substitutions covering the -2 to +2 positions around the mutation and identify one crRNA that can efficiently increase the detection specificity by 13-fold over the ancestral crRNA. With this selected crRNA, the symRNA-Cas12a assay can detect as low as 10 copies of synthetic mutant RNA and the results are confirmed to be accurate by Sanger sequencing. Overall, we have developed the symRNA-Cas12a method to specifically, sensitively and rapidly detect the SARS-CoV-2 D614G mutation.
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http://dx.doi.org/10.1002/biot.202100040DOI Listing
February 2021

Implementing a comprehensive approach to study the causes of human-bear (Ursus arctos pruinosus) conflicts in the Sanjiangyuan region, China.

Sci Total Environ 2021 Jun 2;772:145012. Epub 2021 Feb 2.

Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China. Electronic address:

Personal injury and property loss caused by wildlife often deteriorates the relationship between humans and animals, prompting retaliatory killings that threaten species survival. Conflicts between humans and Tibetan brown bears (Ursus arctos pruinosus) (Human-Bear Conflicts, HBC) in the Sanjiangyuan region have recently dramatically increased, seriously affecting community enthusiasm for brown bears and the conservation of other species. In order to understand the driving mechanisms of HBC, we proposed six potential drivers leading to increased occurrences of HBC. We conducted field research in Zhiduo County of the Sanjiangyuan region from 2017 to 2019 to test hypotheses through semi-constructed interviews, marmot (Marmota himalayana) density surveys and brown bear diet analysis based on metagenomic sequencing. Analysis of herder perceptions revealed that the driving factors of HBC were related to changes in their settlement practice and living habits, changes in foraging behavior of brown bears and recovery of the brown bear population. Since the establishment of winter homes, brown bears have gradually learned to utilize the food in unattended homes. Although 91.4% (n = 285) of the respondents no longer store food in unattended homes, brown bears were reported to still frequently approach winter homes for food due to improper disposal of dead livestock and household garbage. The frequency and abundance of marmots were found to be high in brown bear diet, indicating that marmots were the bears' primary food. However, marmot density had no significant effect on brown bears utilizing human food (P = 0.329), and HBC appears to not be caused by natural food shortages. Distance to rocky outcrops (P = 0.022) and winter homes (P = 0.040) were the key factors linked to brown bears pursuing human food. The number of brown bears has increased over the past decade, and HBC is likely linked to its population recovery. Our findings will provide scientific basis for formulating effective mitigation measures and protection countermeasures for brown bears.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145012DOI Listing
June 2021

The Predictive Values of Different Small Vessel Disease Scores on Clinical Outcomes in Mild ICH Patients.

J Atheroscler Thromb 2021 Feb 6. Epub 2021 Feb 6.

Department of Neurology, Ruijin Hospital/Luwan Branch, School of Medicine, Shanghai Jiao Tong University.

Aim: To explore the predictive values of different small vessel disease (SVD) scores on functional recoveries and the clinical cerebrovascular events in mild intracerebral hemorrhage (ICH).

Methods: In this study, we enrolled conscious and mild ICH patients without surgery and further divided them into the cerebral amyloid angiopathy (CAA)-ICH group and hypertension (HTN)-ICH group. The severity of individual SVD markers, including lacunes, cerebral microbleeds (CMBs), enlarged perivascular spaces (EPVS), white matter hyperintensity (WMH), and cortical superficial siderosis (cSS), was evaluated. The original SVD score, modified SVD score, refined SVD score, and CAA-SVD score and the total number of SVD markers were further calculated. Functional recoveries were evaluated using the modified Rankin scale. Recurrences of stroke were defined as readmission to the hospital with a definite diagnosis of stroke.

Results: A total of 163 ICH patients (60 CAA-ICH and 103 HTN-ICH) were included in the study. The CAA-SVD score (OR=3.429; 95% confidence interval (CI)=1.518-7.748) had the best predictive effect on functional dependence in the CAA-ICH group, among which cSS severities probably played a vital role (OR=4.665; 95% CI=1.388-15.679). The total number of SVD markers [hazard ratio (HR)=3.765; 95% CI=1.467-9.663] can better identify stroke recurrences in CAA-ICH. In HTN-ICH, while the total number of SVD markers (HR=2.136; 95% CI=1.218-3.745) also demonstrated association with recurrent stroke, this effect seems to be related with the influence of lacunes (HR=5.064; 95% CI=1.697-15.116).

Conclusions: The CAA-SVD score and the total number of SVD markers might identify mild CAA-ICH patients with poor prognosis. However, it would be better to focus on lacunes rather than on the overall burden of SVD to predict recurrent strokes in HTN-ICH.
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http://dx.doi.org/10.5551/jat.61267DOI Listing
February 2021

Catalpol-Induced AMPK Activation Alleviates Cisplatin-Induced Nephrotoxicity through the Mitochondrial-Dependent Pathway without Compromising Its Anticancer Properties.

Oxid Med Cell Longev 2021 15;2021:7467156. Epub 2021 Jan 15.

Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, Liaoning, China.

Nephrotoxicity is a common complication of cisplatin chemotherapy and, thus, limits the clinical application of cisplatin. In this work, the effects of catalpol (CAT), a bioactive ingredient extracted from Rehmannia glutinosa, on cisplatin-induced nephrotoxicity and antitumor efficacy were comprehensively investigated. Specifically, the protective effect of CAT on cisplatin-induced injury was explored in mice and HK-2 cells. , CAT administration strikingly suppressed cisplatin-induced renal dysfunction, morphology damage, apoptosis, and inflammation. , CAT induced activation of adenosine 5'-monophosphate- (AMP-) activated protein kinase (AMPK), improved mitochondrial function, and decreased generation of cellular reactive oxygen species (ROS), leading to a reduction in inflammation and apoptosis, which ultimately protected from cisplatin-induced injury. However, the beneficial effects of CAT were mostly blocked by coincubation with compound C. Furthermore, molecular docking results indicated that CAT had a higher affinity for AMPK than other AMPK activators such as danthron, phenformin, and metformin. Importantly, CAT possessed the ability to reverse drug resistance without compromising the antitumor properties of cisplatin. These findings suggest that CAT exerts positive effects against cisplatin-induced renal injury through reversing drug resistance via the mitochondrial-dependent pathway without affecting the anticancer activity of cisplatin.
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http://dx.doi.org/10.1155/2021/7467156DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826214PMC
January 2021

Photo-Oxidation-Controlled Surface Pattern with Responsive Wrinkled Topography and Fluorescence.

Chemistry 2021 Mar 1;27(18):5810-5816. Epub 2021 Mar 1.

School of Chemistry & Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory for Metal Matrix Composite Materials, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.

Wrinkles and photo-oxidation reactions are widely found in soft materials, which are intimately associated with the failure of materials and structures. It is expected that the photo-oxidation process could also have a positive effect on the material and its surface. Here, we report the photo-oxidation of 2-(4-dietheylaminophenyl)-4,5-bis(4-methoxyphenyl) imidazole (DEA-TAI) into a wrinkled bilayer system to control surface wrinkle and fluorescent patterns, in which a supramolecular polymer network composed of carboxylic acid-containing copolymer (PS-BA-AA; PS=poly(styrene), BA=butyl acrylate; AA=acrylic acid) and DEA-TAI were used as the skin layer. Ultraviolet (UV) irradiation can induce photo-oxidation of the imidazole ring of DEA-TAI to weaken the intermolecular hydrogen bonding between PS-BA-AA and DEA-TAI, resulting in the release of stress in the bilayer system. The wrinkled morphology and fluorescence of the surface can be simultaneously regulated by photo-oxidation of DEA-TAI under UV light, and the resulting wrinkles are extremely sensitive to the pH value, which can be quickly and reversibly erased by NH gas. Smart surfaces with specific hierarchical wrinkles and fluorescence can be achieved by selective irradiation with photomasks, which may find potential applications in smart displays and multi-code information storage.
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http://dx.doi.org/10.1002/chem.202100189DOI Listing
March 2021

Design, synthesis and biological evaluation of novel benzoxaborole derivatives as potent PDE4 inhibitors for topical treatment of atopic dermatitis.

Eur J Med Chem 2021 Mar 12;213:113171. Epub 2021 Jan 12.

Department of Medicinal Chemistry, China Pharmaceutical University, Nanjing, 211198, China; Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 21009, China. Electronic address:

In this work, a series of structurally novel benzoxaborole derivatives were designed, synthesized and biologically evaluated as PDE4 inhibitors for battling atopic dermatitis (AD). Among them, the majority exhibited superior PDE4B inhibitory activities to that of the lead compound Crisaborole, an approved PDE4 inhibitor. In particular, 72, the most potent PDE4B inhibitor throughout this series, displayed 136-fold improved enzymatic activity (IC = 0.42 nM) as compared to Crisaborole (IC = 57.20 nM), along with favorable isoform specificity. In the phorbol ester (PMA)-induced mouse ear oedema model, 72 exerted remarkably greater efficacy than Crisaborole at the same dosage (P < 0.05). Moreover, the ointment of 72 exerted dramatically enhanced therapeutic potency than the ointment of Crisaborole (P < 0.05) in the calcipotriol-induced mouse AD model. In addition to the potent in vitro and in vivo activity, 72 displayed favorable safety in the repeated oral dose toxicity study and did not exhibit phototoxicity. With the above attractive biological performance, 72 is worthy of further functional investigation as a novel anti-AD therapeutic agent.
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http://dx.doi.org/10.1016/j.ejmech.2021.113171DOI Listing
March 2021

Light-Induced Programmable 2D Ordered Patterns Based on a Hyperbranched Poly(ether amine) (hPEA)-Functionalized Graphene Film.

ACS Appl Mater Interfaces 2021 Jan 21;13(1):1704-1713. Epub 2020 Dec 21.

School of Chemistry & Chemical Engineering, Frontiers Science Center for Transformative Molecules, State Key Laboratory for Metal Matrix Composite Materials, Shanghai Jiao Tong University, Shanghai 200240, China.

Dynamic complex surface topography with ordered and tunable morphologies, which can provide on-demand control of surface properties to realize smart surfaces, is gaining much attention yet remains challenging in terms of fabrication. Here, a facile, robust, and controllable method is demonstrated to fabricate programmable two-dimensional (2D) ordered patterns with multiresponsive 2D ultrathin materials, comprised of anthracene-capped hyperbranched poly(ether amine) (hPEA-AN)-functionalized graphene (hPEA-AN@G). By combining the stimuli-responsiveness and UV sensitivity of hPEA-AN and excellent out-of-plane deformation and NIR-to-thermal conversion of graphene, the process of "writing/uploading" initial information is conducted through the initial exposure to 365 nm UV light to generate the 2D ordered pattern first; second, inducing swelling strain via moisture to create the hierarchical topographic pattern (orderly oriented pattern) is the process of "modification and erasable rewriting"; third, alternating NIR or 254 nm UV light blanket exposure are the two ways of erasing the information. Consequently, taking advantage of the multiresponsive dynamic wrinkling/ordered patterning, we can program globally 2D ordered surface patterns with diverse morphologies on demand and manipulate the resulted surface properties as desired.
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http://dx.doi.org/10.1021/acsami.0c15099DOI Listing
January 2021

Selaginellin B induces apoptosis and autophagy in pancreatic cancer cells via the JAK2/STAT3 signaling pathway.

Am J Transl Res 2020 15;12(11):7127-7143. Epub 2020 Nov 15.

Department of Biochemistry and Molecular Biology, Dalian Medical University Dalian, China.

Selaginella tamariscina (ST), a well-known traditional medicinal plant, has been used to treat various cancers, including pancreatic cancer. However, the underlying mechanism by which Selaginellin B, a natural pigment isolated and purified from ST, protects against pancreatic cells has yet to be fully elucidated. In the present study, the biological functions of Selaginellin B were investigated using apoptosis, migration and colony formation assays in ASPC-1 and PANC-1 cells. In addition, apoptosis-associated proteins were detected by Western blotting. Our results demonstrated that Selaginellin B induced apoptosis, as evidenced by the increased cleaved caspase-3 level and Bax/Bcl-2 ratio. Moreover, Selaginellin B led to a marked up-regulation of the ratio of LC3-II/LC3-I in ASPC-1 and PANC-1 cells, respectively. Furthermore, reverse pharmacophore screening, molecular docking and molecular dynamics simulation studies revealed that Janus kinase 2 (JAK2) may be a potential target for Selaginellin B. In summary, the results of the present research have demonstrated that Selaginellin B is an effective anticancer agent against PANC-1 and ASPC-1 cells, and the compound holds great promise for the treatment of pancreatic cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724349PMC
November 2020

Synthesis and biological activity of imidazole group-substituted arylaminopyrimidines (IAAPs) as potent BTK inhibitors against B-cell lymphoma and AML.

Bioorg Chem 2021 Jan 29;106:104385. Epub 2020 Oct 29.

College of Laboratory Medicine, College of Pharmacy, Dalian Medical University, Dalian, PR China. Electronic address:

Bruton's tyrosine kinase (BTK) is a member of the Tec kinase family and plays a key role in the modulation of the B-cell receptor (BCR)-mediated signaling pathway. Inhibition of BTK has been proven to be an effective therapeutic approach for various hematological malignancies, such as chronic lymphocytic leukemia (CLL), mantle cell leukemia (MCL), diffuse large B-cell lymphoma (DLBCL) and acute myeloid leukemia (AML). Here, a new series of imidazole group-substituted arylaminopyrimidines (IAAPs) were designed and synthesized as potent inhibitors of the enzymatic activity of BTK with a half maximal inhibitory concentration (IC) ranging from 13.10 to 42.40 nM. In particular, 11a and 11b exhibited stronger antiproliferative activity against AML and B lymphomas cell lines compared with BTK inhibitor ibrutinib and showed low cytotoxicity against normal peripheral blood mononuclear cells (PBMCs). In addition, analysis of the mechanism of action of these compounds revealed that 11a and 11b induced significant apoptosis in AML and B lymphoma cells by arresting the cell cycle at the G1/G0 or G2/M stage and blocked BTK autophosphorylation as well as the ensuing abrogation of pro-survival AKT and ERK signaling. Taken together, these results suggest that 11a and 11b might serve as valuable preclinical candidates for the treatment of AML and B-cell lymphoma.
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http://dx.doi.org/10.1016/j.bioorg.2020.104385DOI Listing
January 2021

Self-Assembled Disulfide Bond Bearing Paclitaxel-Camptothecin Prodrug Nanoparticle for Lung Cancer Therapy.

Pharmaceutics 2020 Dec 1;12(12). Epub 2020 Dec 1.

Department of Radiology Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212001, China.

Self-assembled prodrugs (SAPDs), which combine prodrug strategy and the merits of self-assembly, not only represent an appealing type of therapeutics, enabling the spontaneous organization of supramolecular nanocomposites with defined structures in aqueous environments, but also provide a new method to formulate existing drugs for more favorable outcomes. To increase drug loading and combination therapy, we covalently conjugated paclitaxel (PTX) and camptothecin (CPT) through a disulfide linker into a prodrug, designated PTX-S-S-CPT. The successful production of PTX-S-S-CPT prodrug was confirmed by nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS). This prodrug spontaneously undergoes precipitation in aqueous surroundings. Taking advantage of a flow-focusing microfluidics platform, the prodrug nanoparticles (NPs) have good monodispersity, with good reproducibility and high yield. The as-prepared prodrug NPs were characterized with dynamic light scattering (DLS) and transmission electron microscopy (TEM), demonstrating spherical morphology of around 200 nm in size. In the end, the self-assembled NPs were added to mouse embryonic fibroblast (MEF), mouse lung adenocarcinoma and Lewis lung carcinoma (LLC) cell lines, and human non-small cell lung cancer cell line A549 to evaluate cell viability and toxicity. Due to the redox response with a disulfide bond, the PTX-S-S-CPT prodrug NPs significantly inhibited cancer cell growth, but had no obvious toxicity to healthy cells. This prodrug strategy is promising for co-delivery of PTX and CPT for lung cancer treatment, with reduced side effects on healthy cells.
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http://dx.doi.org/10.3390/pharmaceutics12121169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7760941PMC
December 2020

Photodynamic Pattern Memory Surfaces with Responsive Wrinkled and Fluorescent Patterns.

Adv Sci (Weinh) 2020 Nov 14;7(22):2002372. Epub 2020 Oct 14.

School of Chemistry & Chemical Engineering Frontiers Science Center for Transformative Molecules State Key Laboratory for Metal Matrix Composite Materials Shanghai Jiao Tong University Shanghai 200240 P. R. China.

Reversible pattern systems, namely pattern memory surfaces, possessing tunable morphology play an important role in the development of smart materials; however, the construction of these surfaces is still extensively challenging because of complicated methodologies or chemical reactions. Herein, a functionalized basement is strategically integrated with a multi-responsive supramolecular network based on hydrogen bonding between aggregation-induced emission luminogens (AIEgens) and copolymers containing amidogen (poly(St--Dm) to establish a bilayer system for near-infrared (NIR)-driven memory dual-pattern, where both the fluorescence emission and wrinkled structures can be concurrently regulated by a noninvasive NIR input. The motion of the AIEgens and photo-to-thermal expansion of the modified base allow temporal erasing of the fluorescent wrinkling patterns. Meanwhile, when exposed to 365 nm UV radiation, the fluorescent patterns can be independently regulated through photocyclization. The fluorescent wrinkling pattern presented herein is successfully demonstrated to promote the level of information security and capacity. This strategy provides a brand-new approach for the development of smart memory interfaces.
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http://dx.doi.org/10.1002/advs.202002372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7675060PMC
November 2020

Hybrids of aurantiamide acetate and isopropylated genipin as potential anti-inflammatory agents: The design, synthesis, and biological evaluation.

Chem Biol Drug Des 2021 Apr 30;97(4):797-808. Epub 2020 Nov 30.

College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.

A novel series of hybrids designed on the basis of aurantiamide acetate and isopropylated genipin were synthesized and biologically evaluated as anti-inflammatory agents. Among them, compound 7o exhibited the best inhibitory activity against TNF-α secretion (IC  = 16.90 μM) and was selected for further in vitro and in vivo functional study. The results demonstrated that 7o was capable of suppressing the expression of LPS-induced iNOS and COX-2, as well as reducing the production of NO at the concentration of 5 μM, which may be resulted from its regulation of NF-κB signaling and MAPK signaling. Moreover, compound 7o exhibited favorable in vivo anti-inflammatory activity with an inhibition rate of 53.32% against xylene-induced ear swelling in mice at the dose of 5 mg/kg.
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http://dx.doi.org/10.1111/cbdd.13809DOI Listing
April 2021

Soil microbial community succession and interactions during combined plant/white-rot fungus remediation of polycyclic aromatic hydrocarbons.

Sci Total Environ 2021 Jan 9;752:142224. Epub 2020 Sep 9.

Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Chinese Academy of Forestry Research Institute of Forestry, Xiangshan Road, Haidian District, Beijing 100091, China. Electronic address:

Despite combined plant/white-rot fungus remediation being effective for remediating polycyclic aromatic hydrocarbon (PAH)-contaminated soil, the complex organismal interactions and their effects on soil PAH degradation remain unclear. Here, we used quantitative PCR, analysis of soil enzyme activities, and sequencing of representative genes to characterize the ecological dynamics of natural attenuation, mycoremediation (MR, using Crucibulum laeve), phytoremediation (PR, using Salix viminalis), and plant-microbial remediation (PMR, using both species) for PAHs in soil for 60 days. On day 60, PMR achieved the highest removal efficiency of all three representative PAHs (65.5%, 47.5%, and 62.4% for phenanthrene, pyrene, and benzo(a)pyrene, respectively) when compared with the other treatments. MR significantly increased the relative abundance of Rhizobium and Bacillus but antagonized the other putative indigenous PAH-degrading bacteria, which were enriched by PR. PR significantly reduced soil nutrients, such as NO and NH, and available potassium (AK), thereby changing the microbial community composition as reflected by redundancy analysis, significantly reducing the soil bacterial biomass relative to that in other treatments. These disadvantages hampered phenanthrene and pyrene removal. MR provided additional nutrients, which counteracted the nutrient consumption associated with PR, thereby maintaining the microbial community diversity and bacterial biomass of PMR at a level achieved in the NA treatment. Combination remediation therefore overcame the disadvantages of using PR alone. These results indicated that inoculation with the combination of S. viminalis and C. laeve synergistically stimulated the growth of indigenous PAH-degrading microorganisms and maintained bacterial biomass, thus accelerating the dissipation of soil PAHs.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142224DOI Listing
January 2021

Connectome-Based Model Predicts Deep Brain Stimulation Outcome in Parkinson's Disease.

Front Comput Neurosci 2020 28;14:571527. Epub 2020 Oct 28.

School of Computer Science and Technology, Beijing Institute of Technology, Beijing, China.

Subthalamic nucleus deep brain stimulation (STN-DBS) is an effective invasive treatment for advanced Parkinson's disease (PD) at present. Due to the invasiveness and cost of operations, a reliable tool is required to predict the outcome of therapy in the clinical decision-making process. This work aims to investigate whether the topological network of functional connectivity states can predict the outcome of DBS without medication. Fifty patients were recruited to extract the features of the brain related to the improvement rate of PD after STN-DBS and to train the machine learning model that can predict the therapy's effect. The functional connectivity analyses suggested that the GBRT model performed best with Pearson's correlations of = 0.65, = 2.58E-07 in medication-off condition. The connections between middle frontal gyrus (MFG) and inferior temporal gyrus (ITG) contribute most in the GBRT model.
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http://dx.doi.org/10.3389/fncom.2020.571527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656054PMC
October 2020

Clinical practice guidelines for the management of adult diffuse gliomas.

Cancer Lett 2021 Feb 6;499:60-72. Epub 2020 Nov 6.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052, China.

To follow the revision of the fourth edition of WHO classification and the recent progress on the management of diffuse gliomas, the joint guideline committee of Chinese Glioma Cooperative Group (CGCG), Society for Neuro-Oncology of China (SNO-China) and Chinese Brain Cancer Association (CBCA) updated the clinical practice guideline. It provides recommendations for diagnostic and management decisions, and for limiting unnecessary treatments and cost. The recommendations focus on molecular and pathological diagnostics, and the main treatment modalities of surgery, radiotherapy, and chemotherapy. In this guideline, we also integrated the results of some clinical trials of immune therapies and target therapies, which we think are ongoing future directions. The guideline should serve as an application for all professionals involved in the management of patients with adult diffuse glioma and also a source of knowledge for insurance companies and other institutions involved in the cost regulation of cancer care in China and other countries.
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http://dx.doi.org/10.1016/j.canlet.2020.10.050DOI Listing
February 2021

Puerarin sensitized K562/ADR cells by inhibiting NF-κB pathway and inducing autophagy.

Phytother Res 2021 Mar 3;35(3):1658-1668. Epub 2020 Nov 3.

Department of Clinical Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, Liaoning, China.

Puerarin is an isoflavone isolated from Pueraria lobata (Willd.) Ohwi. In the present study, reversal effect and underlying mechanisms of puerarin on multidrug resistance (MDR) were investigated in K562/ADR cells. K562/ADR cells exhibited adriamycin (ADR) resistance and higher levels of MDR1 expression compared with K562 cells. Puerarin enhanced the chemosensitivity of K562/ADR cells and increased the ADR accumulation in K562/ADR cells. The expression levels of MDR1 were down-regulated by puerarin in K562/ADR cells. Luciferase reporter assay further demonstrated the inhibitory effect of puerarin on TNF-α-induced NF-κB activation. The phosphorylation of IκB-α was significantly suppressed by puerarin. In silico docking analyses suggested that puerarin well matched with the active sites of IκB-α. Moreover, a large number of autophagosomes were found in the cytoplasm of K562/ADR cells after puerarin treatment. The significant increase in LC3-II and beclin-1 was also observed, indicating autophagy induction by puerarin in K562/ADR cells. Puerarin induced cell cycle arrest and apoptosis in K562/ADR cells. Finally, puerarin inhibited phosphorylation of Akt and JNK. In conclusion, puerarin-sensitized K562/ADR cells by downregulating MDR1 expression via inhibition of NF-κB pathway and autophagy induction via Akt inhibition.
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http://dx.doi.org/10.1002/ptr.6932DOI Listing
March 2021

Light-Promoted Copper-Catalyzed Enantioselective Alkylation of Azoles.

Angew Chem Int Ed Engl 2021 Jan 20;60(4):2130-2134. Epub 2020 Nov 20.

State Key Laboratory of Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Center for Excellence in Molecular Synthesis, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, P. R. China.

A catalytic asymmetric alkylation of azoles with secondary 1-arylalkyl bromides through direct C-H functionalization is reported. Under blue-light photoexcitation, a copper(I)/carbazole-based bisoxazoline (CbzBox) catalytic system exhibits good reactivity and high stereoselectivity, thus offering an efficient strategy for the construction of chiral alkyl azoles. These reactions proceed at low temperature and are compatible with a wide range of azoles.
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http://dx.doi.org/10.1002/anie.202009323DOI Listing
January 2021

Systematic analysis of gut microbiota in pregnant women and its correlations with individual heterogeneity.

NPJ Biofilms Microbiomes 2020 09 11;6(1):32. Epub 2020 Sep 11.

School of Public Health, Guangzhou Medical University, 511436, Guangzhou, China.

The woman's gut microbiota during pregnancy may support nutrient acquisition, is associated with diseases, and has been linked to infant health. However, there is limited information on gut microbial characteristics and dependence in pregnant women. In this study, we provide a comprehensive overview of the gut microbial characteristics of 1479 pregnant women using 16S rRNA gene sequencing of fecal samples. We identify a core microbiota of pregnant women, which displays a similar overall structure to that of age-matched nonpregnant women. Our data show that the gestational age-associated variation in the gut microbiota, from the ninth week of gestation to antepartum, is relatively limited. Building upon rich metadata, we reveal a set of exogenous and intrinsic host factors that are highly correlated with the variation in gut microbial community composition and function. These microbiota covariates are concentrated in basic host properties (e.g., age and residency status) and blood clinical parameters, suggesting that individual heterogeneity is the major force shaping the gut microbiome during pregnancy. Moreover, we identify microbial and functional markers that are associated with age, pre-pregnancy body mass index, residency status, and pre-pregnancy and gestational diseases. The gut microbiota during pregnancy is also different between women with high or low gestational weight gain. Our study demonstrates the structure, gestational age-associated variation, and associations with host factors of the gut microbiota during pregnancy and strengthens the understanding of microbe-host interactions. The results from this study offer new materials and prospects for gut microbiome research in clinical and diagnostic fields.
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http://dx.doi.org/10.1038/s41522-020-00142-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486914PMC
September 2020

Targeting PD-L1 in non-small cell lung cancer using CAR T cells.

Oncogenesis 2020 Aug 13;9(8):72. Epub 2020 Aug 13.

Department of Medicine, Baylor College of Medicine, Houston, TX, 77030, USA.

Antibodies against programmed cell death protein 1 (PD-1) and its ligand (PD-L1) have dramatically changed the landscape of therapies for non-small cell lung carcinoma (NSCLC); however, the majority of patients do not respond to these agents. In addition, hyperprogressive disease (HPD) develops in a larger portion of NSCLC patients treated with PD-1/PD-L1 inhibitors than in patients treated with standard chemotherapy. The use of chimeric antigen receptor (CAR) T cells has been successful to treat blood cancers but not for solid tumors like NSCLC. In this work, we constructed CAR T cells that target PD-L1 and evaluated their efficacy in NSCLC with either high or low PD-L1 expression. PD-L1-CAR T cells exhibited antigen-specific activation, cytokine production, and cytotoxic activity against PD-L1 NSCLC cells and xenograft tumors. Furthermore, the addition of a subtherapeutic dose of local radiotherapy improved the efficacy of PD-L1-CAR T cells against PD-L1 NSCLC cells and tumors. Our findings indicate that PD-L1-CAR T cells represent a novel therapeutic strategy for patients with PD-L1-positive NSCLC, particularly for those who are susceptible to HPD.
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http://dx.doi.org/10.1038/s41389-020-00257-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426958PMC
August 2020

Inhibition of p66Shc Oxidative Signaling via CA-Induced Upregulation of miR-203a-3p Alleviates Liver Fibrosis Progression.

Mol Ther Nucleic Acids 2020 Sep 10;21:751-763. Epub 2020 Jul 10.

Department of Pharmacology, Dalian Medical University, Dalian 116044, China. Electronic address:

We previously found that inhibition of p66Shc confers protection against hepatic stellate cell (HSC) activation during liver fibrosis. However, the effect of p66Shc on HSC proliferation, as well as the mechanism by which p66Shc is modulated, remains unknown. Here, we elucidated the effect of p66Shc on HSC proliferation and evaluated microRNA (miRNA)-p66Shc-mediated reactive oxidative species (ROS) generation in liver fibrosis. An in vivo model of carbon tetrachloride (CCl)-induced liver fibrosis in rats and an LX-2 cell model were developed. p66Shc expression was significantly upregulated in rats with CCl-induced liver fibrosis and in human fibrotic livers. Additionally, p66Shc knockdown in vitro attenuated mitochondrial ROS generation and HSC proliferation. Interestingly, p66Shc promoted HSC proliferation via β-catenin dephosphorylation in vitro. MicroRNA (miR)-203a-3p, which was identified by microarray and bioinformatics analyses, directly inhibited p66Shc translation and attenuated HSC proliferation in vitro. Importantly, p66Shc was found to play an indispensable role in the protective effect of miR-203a-3p. Furthermore, carnosic acid (CA), the major antioxidant compound extracted from rosemary leaves, protected against CCl-induced liver fibrosis through the miR-203a-3p/p66Shc axis. Collectively, these results suggest that p66Shc, which is directly suppressed by miR-203a-3p, is a key regulator of liver fibrosis. This finding may lead to the development of therapeutic targets for liver fibrosis.
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http://dx.doi.org/10.1016/j.omtn.2020.07.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417942PMC
September 2020

Transcriptomic and metabolomic insights into the adaptive response of Salix viminalis to phenanthrene.

Chemosphere 2021 Jan 9;262:127573. Epub 2020 Jul 9.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China. Electronic address:

Polycyclic aromatic hydrocarbons (PAHs) are widespread, persistent environmental pollutants. They exert toxic effects at different developmental stages of plants. Plant defense mechanisms against PAHs are poorly understood. To this end, transcriptomics and widely targeted metabolomic sequencing were used to study the changes in gene expression and metabolites that occur in the roots of Salix viminalis subjected to phenanthrene stress. Significant variations in genes and metabolites were observed between treatment groups and the control group. Thirteen amino acids and key genes involved in their biosynthesis were upregulated exposed to phenanthrene. Cysteine biosynthesis was upregulated. Sucrose, inositol galactoside, and mellidiose were the main carbohydrates that were largely accumulated. Glutathione biosynthesis was enhanced in order to scavenge reactive oxygen species and detoxify the phenanthrene. Glucosinolate and flavonoid biosynthesis were upregulated. The production of pinocembrin, apigenin, and epigallocatechin increased, which may play a role in antioxidation to resist phenanthrene stress. In addition, levels of six amino acids and N,N'-(p-coumaroyl)-cinnamoyl-caffeoyl-spermidine were significantly increased, which may have helped protect the plant against phenanthrene stress. These results demonstrated that S. viminalis had a positive defense strategy in response to phenanthrene challenge. Subsequent defense-related reactions may have also occurred within 24 h of phenanthrene exposure. The findings of the present study would be useful in elucidating the molecular mechanisms regulating plant responses to PAH challenges and would help guide crop and plant breeders in enhancing PAH resistance.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127573DOI Listing
January 2021

Identification of Potential Key Genes and Pathways in Enzalutamide-Resistant Prostate Cancer Cell Lines: A Bioinformatics Analysis with Data from the Gene Expression Omnibus (GEO) Database.

Biomed Res Int 2020 16;2020:8341097. Epub 2020 Jul 16.

Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi 710004, China.

Enzalutamide (ENZ) has been approved for the treatment of advanced prostate cancer (PCa), but some patients develop ENZ resistance initially or after long-term administration. Although a few key genes have been discovered by previous efforts, the complete mechanisms of ENZ resistance remain unsolved. To further identify more potential key genes and pathways in the development of ENZ resistance, we employed the GSE104935 dataset, including 5 ENZ-resistant (ENZ-R) and 5 ENZ-sensitive (ENZ-S) PCa cell lines, from the Gene Expression Omnibus (GEO) database. Integrated bioinformatics analyses were conducted, such as analysis of differentially expressed genes (DEGs), Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, protein-protein interaction (PPI) analysis, gene set enrichment analysis (GSEA), and survival analysis. From these, we identified 201 DEGs (93 upregulated and 108 downregulated) and 12 hub genes (AR, ACKR3, GPER1, CCR7, NMU, NDRG1, FKBP5, NKX3-1, GAL, LPAR3, F2RL1, and PTGFR) that are potentially associated with ENZ resistance. One upregulated pathway (hedgehog pathway) and seven downregulated pathways (pathways related to androgen response, p53, estrogen response, TNF-, TGF-, complement, and pancreas cells) were identified as potential key pathways involved in the occurrence of ENZ resistance. Our findings may contribute to further understanding the molecular mechanisms of ENZ resistance and provide some clues for the prevention and treatment of ENZ resistance.
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http://dx.doi.org/10.1155/2020/8341097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382728PMC
April 2021

Selective adsorption of PHC and regeneration of washing effluents by modified diatomite.

Water Sci Technol 2020 May;81(10):2066-2077

PetroChina Dagang Oilfield Company, Tianjin, 300280, China.

Selective removal of petroleum hydrocarbons (PHCs) from soil washing effluents is the key to the surfactant-enhanced soil washing technology. In this study, the diatomite was modified by nonionic surfactant TX-100 and applied in the selective adsorption of PHCs in the soil washing effluents. The modified diatomites were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, N adsorption/desorption and X-ray photoelectron spectroscopy respectively. The adsorption process followed the pseudo-second-order model and the adsorption isotherms indicated that the interaction between PHCs and modified diatomite was monolayer adsorption. The important operating factors such as TX-100 dosage, adsorbent dosage, time and temperature were optimized. With the participation of the low-cost adsorbent TX3-Db with high adsorption capacity, the recovery efficiency of the washing effluents was still up to 78.9% after three cycles. A selective adsorption mechanism, based on steric hindrance and electrostatic repulsion, was proposed to explain the removal of PHCs from washing effluents.
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http://dx.doi.org/10.2166/wst.2020.262DOI Listing
May 2020

Enhancing Salix viminalis L.-mediated phytoremediation of polycyclic aromatic hydrocarbon-contaminated soil by inoculation with Crucibulum laeve (white-rot fungus).

Environ Sci Pollut Res Int 2020 Nov 17;27(33):41326-41341. Epub 2020 Jul 17.

Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China.

Although plant-white-rot fungi (WRF) remediation is considered efficient in improving polycyclic aromatic hydrocarbon (PAH)-contaminated soil, the prospects for using it remain poorly known. Therefore, we evaluated whether the WRF Crucibulum laeve could improve the phytoremediation of PAH-contaminated soil by Salix viminalis L. A 60-day pot experiment was conducted to investigate the effects of C. laeve inoculation (using two inoculation treatments and a non-inoculated control) on the phytoremediation potential, growth, and antioxidant metabolism of S. viminalis cultivated in PAH-contaminated soil. The S. viminalis-C. laeve association synergistically caused the highest PAH removal rate. Under the S. viminalis-C. laeve treatment, 80% of the biological concentration and translocation factors for all tissues of S. viminalis were > 1, whereas only 20% of these factors were > 1 when S. viminalis was used alone. C. laeve inoculation remarkably enhanced phytoremediation by promoting S. viminalis-based phytoextraction of PAHs from soils. Furthermore, although C. laeve inoculation altered the antioxidant metabolism of S. viminalis by inducing oxidative stress, thereby inhibiting plant growth, the plant's hardiness enabled it to survive and grow normally for 60 days after treatment. Therefore, phytoremediation using S. viminalis inoculated with C. laeve can be considered a feasible approach for the phytoremediation of PAH-contaminated soil.
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http://dx.doi.org/10.1007/s11356-020-10125-3DOI Listing
November 2020

LncRNA Mical2/miR-203a-3p sponge participates in epithelial-mesenchymal transition by targeting p66Shc in liver fibrosis.

Toxicol Appl Pharmacol 2020 09 11;403:115125. Epub 2020 Jul 11.

Department of Pharmacology, Dalian Medical University, Dalian 116044, China. Electronic address:

Epithelial-mesenchymal transition (EMT) is regulated by reactive oxygen species (ROS) in liver fibrosis. p66Shc is a redox enzyme, but its role of EMT is unclear in liver fibrosis. Long noncoding RNAs (lncRNAs) have been implicated as important regulators in numerous physiological and pathological processes and generally acting as a microRNA (miRNA) sponge to regulate gene expression. The aim of the current study was to evaluate the contribution of p66Shc to EMT in liver fibrosis and the regulation of p66Shc by lncRNA sponge. In vivo, p66Shc silencing prevented carbon tetrachloride (CCl)-induced EMT as evidenced by the upregulation of E-cadherin, downregulation of Vimentin and N-cadherin, and inhibition of oxidative stress and extracellular matrix (ECM) components. Moreover, in vitro, TGF-β1 significantly enhanced ECM components, as well as the development of the EMT phenotype. These effects were abrogated by p66Shc downregulation and aggravated by p66Shc overexpression. Mechanistically, p66Shc contributed to EMT via mediating ROS, as evidenced by p66Shc downregulation inhibiting EMT under exogenous hydrogen peroxide (HO) stimulation. Furthermore, we found that molecule interacting with CasL2 (Mical2) lncRNA functioned as an endogenous miR-203a-3p sponge to regulate p66Shc expression. Both Mical2 silencing and miR-203a-3p agomiR treatment downregulated p66Shc expression, thus suppressing EMT in vivo and in vitro. Notably, the increased p66Shc and Mical2 levels and decreased miR-203a-3p levels in murine fibrosis were consistent with those in patients with liver fibrosis. In sum, our study reveals that p66Shc is critical for liver fibrosis and that Mical2, miR-203a-3p and p66Shc compose a novel regulatory pathway in liver fibrosis.
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http://dx.doi.org/10.1016/j.taap.2020.115125DOI Listing
September 2020

Perfluoroalkyl substances in the Lingang hybrid constructed wetland, Tianjin, China: occurrence, distribution characteristics, and ecological risks.

Environ Sci Pollut Res Int 2020 Nov 5;27(31):38580-38590. Epub 2020 Jul 5.

Tianjin Lingang Construction Development Co., Ltd, Tianjin, 300450, China.

In this study, the occurrence, spatial distribution, sources, and ecological risks of perfluoroalkyl substances (PFASs) in the surface waters of the Lingang hybrid constructed wetland were systematically investigated. Twenty-three PFASs were analyzed from 7 representative sampling zones. The obtained results indicated that PFBA, PFPeA, PFHxA, PFHpA, PFOA, PFBS, PFOS, and HFPO-DA were frequently detected; and PFBA, PFOA, and PFOS were the dominant PFASs with the relative abundances in ranges of 26.91 to 52.26%, 11.79 to 28.79%, and 0 to 31.98%, respectively. The total concentrations of 8 PFASs (ΣPFASs) ranged from 25.9 to 56.6 ng/L, and the highest concentration was observed in subsurface flow wetland. Moreover, HFPO-DA with high toxicity was detected in wetlands for the first time. Based on the principal component analysis-multiple linear regression (PCA-MLR) analysis, three sources and their contributions were fluoropolymer processing aids (67.6%), fluororesin coatings and metal plating (17.9%), and food packaging materials and atmospheric precipitation (14.5%), respectively. According to the risk quotients (RQs), the ecological risk of 8 PFASs was low to the aquatic organisms.
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http://dx.doi.org/10.1007/s11356-020-09921-8DOI Listing
November 2020

CD96, a new immune checkpoint, correlates with immune profile and clinical outcome of glioma.

Sci Rep 2020 07 1;10(1):10768. Epub 2020 Jul 1.

Department of Neurosurgery, Xiangya Hospital, Central South University, Central South University, 87 Xiangya Road, Changsha, Hunan, 410008, China.

CD96 is a promising candidate for immunotherapy. However, its role and importance in glioma remains unknown. We thus aimed to genetically and clinically characterize CD96 expression in gliomas. For this, we extracted RNA-seq data of 699 glioma samples from the TCGA dataset and validated these findings using the CGGA dataset comprising 325 glioma samples. Clinical and isocitrate dehydrogenase (IDH) mutation status were also analyzed. Various packages in R language were mainly used for statistical analysis. CD96 expression was significantly up-regulated in high-grade, IDH-wildtype, and mesenchymal-molecular subtype gliomas based on TCGA data, which was validated using the CGGA dataset. Subsequent gene ontology analysis of both datasets suggested that genes relevant to CD96 are mainly involved in immune functions in glioma as such genes were positively correlated with CD96 expression. To further explore the relationship between CD96 and immune responses, we selected seven immune-related metagenes and found that CD96 expression was positively correlated with HCK, LCK, and MHC II in the CGGA and TCGA cohorts but negatively associated with IgG. Further, Pearson correlation analysis showed that CD96 is associated with TIGIT, CD226, CRTAM, TIM-3, PD-L1, CTLA-4, and STAT3, indicating the additive antitumoral effects of these checkpoint proteins. CD96 was also suggested to play an important role in immune responses and positively collaborate with other checkpoint members. These findings show that CD96 is promising candidate for immunotherapy, and that such agents could complement current immunotherapy strategies for glioma.
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http://dx.doi.org/10.1038/s41598-020-66806-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330044PMC
July 2020