Publications by authors named "Xiaobo Zhang"

476 Publications

Letter to the editor on "Outcomes of ossicle resection and anatomic reconstruction of lateral ligaments for chronic ankle instability with large malleolar accessory ossicles".

Foot Ankle Surg 2021 Oct 11. Epub 2021 Oct 11.

Department of Orthopaedics, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou Workers' Hospital, Liuzhou 545005, Guangxi, China.

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http://dx.doi.org/10.1016/j.fas.2021.10.005DOI Listing
October 2021

Influence of the Environment on the Distribution and Quality of Fisch.

Front Plant Sci 2021 27;12:706822. Epub 2021 Sep 27.

Baotou Medical College, Inner Mongolia, Baotou, China.

Fisch. is a characteristic medicinal plant found in Inner Mongolia, China. To meet the increase in market demand and promote the development of medicinal plant science, we explored the influence of the environment on its distribution and the quantity of its active compounds (loganic acid and 6'---D-glucosylgentiopicroside) to find suitable cultivation areas for . Based on the geographical distribution of in Inner Mongolia and the ecological factors that affect its growth, identified from the literature and field visits, a boosted regression tree (BRT) was used to model ecologically suitable areas in the region. The relationship between the content of each of active compound in the plant and ecological factors was also established for Inner Mongolia using linear regression. The results showed that elevation and soil type had the most significant influence on the distribution of -their relative contribution was 30.188% and 28.947%, respectively. The factors that had the greatest impact on the distribution of high-quality were annual precipitation, annual mean temperature, and temperature seasonality. The results of BRT and linear regression modeling showed that suitable areas for high-quality included eastern Ordos, southern Baotou, Hohhot, southern Wulanchabu, southern Xilin Gol, and central Chifeng. However, there were no significant correlations between the contents of loganic acid and 6'---D-glucosylgentiopicroside and the ecological factors. This study explored the influence of the environment on the growth and quantity of active compounds in to provide guidance for coordinating the development of medicinal plant science.
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http://dx.doi.org/10.3389/fpls.2021.706822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503573PMC
September 2021

Requirement of transcription factor NME2 for the maintenance of the stemness of gastric cancer stem-like cells.

Cell Death Dis 2021 Oct 9;12(10):924. Epub 2021 Oct 9.

College of Life Sciences and Laboratory for Marine Biology and Biotechnology of Pilot National Laboratory for Marine Science and Technology (Qingdao), Zhejiang University, Hangzhou, 310058, People's Republic of China.

Cancer stem cells (CSCs), which can self-renew and produce heterogeneous cancer cells, are the key factors during tumorigenesis. Transcription factors take essential effects on CSCs. However, the role of transcription factors in regulating the stemness of gastric cancer stem-like cells has not been well explored. In this investigation, it was found that transcription factor NME2 (NME/NM23 nucleoside diphosphate kinase 2) was upregulated in gastric cancer stem-like cells that sorted from the solid tumors of patients with gastric cancer and gastric cancer cell lines. NME2 could preserve the stemness of gastric cancer stem-like cells via suppressing their apoptosis. In vitro and in vivo data revealed that NME2 was crucial for maintaining the stemness of gastric cancer stem cells by enhancing the expression of anti-apoptosis genes. Consequently, our data contributed a new perspective to the relationship between transcription factor and the stemness maintenance of gastric cancer stem cells.
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http://dx.doi.org/10.1038/s41419-021-04234-1DOI Listing
October 2021

An Optimized Pathway for the Differential Diagnosis of ACTH-Dependent Cushing's Syndrome Based on Low-Dose Dexamethasone Suppression Test.

Front Endocrinol (Lausanne) 2021 2;12:720823. Epub 2021 Sep 2.

Department of Endocrinology, Key Laboratory of Endocrinology of National Health Commission, Translation Medicine Centre, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Context: Traditionally, low-dose dexamethasone suppression test (LDDST) was used to confirm the diagnosis of Cushing's syndrome (CS), and high-dose dexamethasone suppression test (HDDST) was used to differentiate Cushing's disease (CD) and ectopic adrenocorticotropin (ACTH) syndrome (EAS), but some studies suggested that HDDST might be replaced by LDDST. For the differential diagnosis of CS, dexamethasone suppression test was usually combined with other tests such as bilateral petrosal sinus sampling (BIPSS) and pituitary magnetic resonance imaging, but the optimal pathway to incorporate these tests is still controversial.

Objectives: To develop an optimized pathway for the differential diagnosis of CD and EAS based on LDDST.

Design And Setting: Single-center retrospective study (2011-2019).

Patients: Two hundred sixty-nine CD and 29 EAS patients with pathological diagnosis who underwent consecutive low- and high-dose DST.

Results: For the differential diagnosis of CD and EAS, the area under curve (AUC) of LDDST using urine free cortisol (0.881) was higher than that using serum cortisol (0.685) (p < 0.001) in head-to-head comparison among a subgroup of 108 CD and 10 EAS. The AUC of LDDST (0.883) was higher than that of HDDST (0.834) among all the included patients. With the cutoff of <26%, the sensitivity and specificity of LDDST were 39.4% and 100%. We designed a new pathway in which BIPSS was only reserved for those patients with unsuppressed LDDST and adenoma <6mm, yielding an overall sensitivity of 97.7% and specificity of 86.7%.

Conclusion: LDDST had similar value to HDDST in differentiating CD and EAS using the specific cutoff point. The pathway that combined LDDST and BIPSS could differentiate CD and EAS accurately.
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http://dx.doi.org/10.3389/fendo.2021.720823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445331PMC
September 2021

Characteristics of childhood allergic diseases in outpatient and emergency departments in Shanghai, China, 2016-2018: a multicenter, retrospective study.

BMC Pediatr 2021 09 17;21(1):409. Epub 2021 Sep 17.

Department of Respiratory Medicine, Children's Hospital of Fudan University, National Children's Medical Center, Shanghai, 201102, China.

Background: The prevalence of allergic diseases (ADs), such as asthma and allergic rhinitis (AR), is increasing worldwide in both adults and children. Although ADs are common and frequently coexist in outpatient care, city-level data regarding the characteristics of childhood AD remain limited in China. This study aimed to assess the profile and characteristics of ADs in the city of Shanghai.

Methods: A multicenter retrospective study was designed to collect routine administrative data from outpatient and emergency departments from 66 hospitals in Shanghai, China, from 2016 to 2018. Children with asthma, AR, allergic conjunctivitis (AC), and allergic skin diseases were investigated. Demographic characteristics, patients visit pattern, spectrum of diagnosis, and comorbidities were analyzed.

Results: A total of 2,376,150 outpatient and emergency visits for ADs were included in the period from 2016 to 2018. Allergic skin diseases accounted for 38.9%, followed by asthma (34.8%), AR (22.9%), and AC (3.3%), with a male predominance in all four diseases. Asthma and allergic skin diseases were most frequent in the 1 to < 4 years of age group, while AR and AC were more common in the 4 to < 7 years of age group. Asthma accounted for the greatest number of annual and emergency visits. The most frequent comorbidity of asthma was lower respiratory tract infection (LRTI) (49.3%), followed by AR (20.5%) and upper respiratory tract infection (14.1%). The most common comorbidities of AR were otitis media (23.4%), adenoid hypertrophy/obstructive sleep apnea (22.1%), followed by LRTI (12.1%), asthma (9.4%) and chronic pharyngitis (8.9%).

Conclusions: Asthma and allergic skin diseases were the most common ADs in outpatient and emergency departments in the study period. Respiratory tract infection was the most common comorbidity of asthma in children. More attention should be devoted to the treatment of comorbidities to improve childhood AD outcomes with a better understanding of the characteristics of ADs in outpatient care.
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http://dx.doi.org/10.1186/s12887-021-02880-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447671PMC
September 2021

Flexible TiCT MXene/PANI/Bacterial Cellulose Aerogel for e-Skins and Gas Sensing.

ACS Appl Mater Interfaces 2021 Sep 15;13(38):45987-45994. Epub 2021 Sep 15.

Department of Instrumentation and Analytical Chemistry, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China.

Flexible pressure sensors made of carbon materials have been used in electronic skins (e-skins), whose performance can be enhanced if composite sensing materials are used. Herein, an MXene/polyaniline/bacterial cellulose (MXene/PANI/BC) aerogel sensor has been fabricated through the self-assembly process between the MXene and one-dimensional active material. Combined with fewer-layer or single-layer MXenes, the as-fabricated aerogel could be used as the active layer of the pressure sensor, monitoring tiny motion signals of finger bending, wrist bending, and pulse beating. Bluetooth wireless transmission could also be realized to monitor the real-time spatial pressure distributions on the mobile phone, making the aerogel-based sensor an ideal candidate in e-skins. Meanwhile, the aerogel-based sensor is sensitive toward NH due to the unique three-dimensional (3D) structure of the aerogel and the abundant terminal groups (such as -O, -OH, and -F) of the MXene in the system that ensure efficient electronic transfer for the sensing process and create active sites for the absorption with the target gas. This work offers a versatile platform to develop MXenes to fabricate 3D composite aerogels for high-performance flexible multiple sensors.
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http://dx.doi.org/10.1021/acsami.1c12991DOI Listing
September 2021

High performance sub-bandgap photodetection internal photoemission based on ideal metal/2D-material van der Waals Schottky interface.

Nanoscale 2021 Oct 14;13(39):16448-16456. Epub 2021 Oct 14.

Key Laboratory of Optoelectronics Technology, Ministry of Education, Faculty of Information Technology, Beijing University of Technology, Beijing 100124, China.

Two-dimensional (2D) materials have been demonstrated to be promising candidates to design high performance photodetectors owing to their strong light-matter interaction. However, the performance of 2D material photodetectors is still unsatisfactory, such as slow response speed due to defects and vulnerable contact interface, which impede their rapid development in the field of optoelectronics. In this paper, we obtained the ideal and large photosensitive van der Waals Schottky interface by the laminating-flipping method. Hence, a fast response speed (<1 ms) and high detectivity (>10 Jones) are observed on the van der Waals Schottky junction photodiode. More importantly, benefiting from the flat Schottky interface (the roughness ∼0.6 nm), a sub-bandgap light response modulated by the Schottky barrier height (cut-off edge at 1050 nm) has been detected based on the large Au/MoSe sensitive Schottky interface internal photoemission. As a result, a universal strategy for the sub-bandgap near-infrared van der Waals Schottky junction detector of 2D materials was obtained.
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http://dx.doi.org/10.1039/d1nr04770aDOI Listing
October 2021

Activating a DNA Nanomachine via Computation across Cancer Cell Membranes for Precise Therapy of Solid Tumors.

J Am Chem Soc 2021 Sep 13;143(37):15233-15242. Epub 2021 Sep 13.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Taking advantage of cancer cells' endogenous characters, the responsive activation of DNA nanomachines has achieved great success in tumor therapy. Combining with extra stimuli such as external light irradiation provided spatiotemporal control of DNA nanomachine activation. However, specific activation at the cellular level is still challenging considering the macroscopic-scale exposure area of usual light sources. DNA logic gates located at the cell membrane contributed to cellular specificity, but the free diffusion of input DNA strands during the operation process would impair efficiency and result in side effects to circumjacent normal cells in solid tumors. Here we design a transmembrane DNA logical computation strategy to activate a DNA nanomachine only in cancer cells from a complex solid tumor microenvironment. The DNA nanomachine multishell UCNPs-DNA is prepared by modifying DNA strands on upconversion nanoparticles. LA-apt, a DNA strand anchoring to a cancer cell membrane overexpressed receptor, and intracellular miRNA-21 served as inputs 1 and 2, respectively. Hybridization with input 1 at the cell membrane not only exposes the miRNA-21 recognition region at the DNA nanomachine, but also delivers it into cancer cells. The cascade hybridization with intracellular input 2 completes the "AND" gate operation and releases a DNA strand L2 as output. L2 acts as the trigger to operate the DNA nanomachine and correspondingly activates the photosensitizer Rose Bengal for reactive oxygen species generation. Through the "AND" gate operation of the DNA nanomachine across the cancer cell membrane, highly precise therapy only to cancer cells is achieved in a complex solid tumor microenvironment, which could become a promising modality for precise therapy of solid tumors.
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http://dx.doi.org/10.1021/jacs.1c06361DOI Listing
September 2021

The BODIPY-based chemosensor for the fluorometric determination of organochlorine pesticide dicofol.

Food Chem 2021 Sep 2;370:131033. Epub 2021 Sep 2.

Department of Instrumentation and Analytical Chemistry, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. Electronic address:

Dicofol is an organochlorine pesticide, which is widely used in fruits, tea and other crops, and is moderately toxic to humans. Therefore, the monitoring of organochlorine pesticide-dicofol is critical for food safety. In this work, a fluorometric chemosensor based on mercaptoethanol and boron dipyrromethene (BODIPY) was first constructed to detect the dicofol. The chemosensor displayed turn-off fluorescence behavior upon dicofol with a detection limit of 200 ppb. The nucleophilicity of the glutathione and other biological thiols was studied to evaluate the reactivity of thiols with dicofol. In practical applications, an obvious color difference was observed on a paper based microfluidic device modified by phenyltriethoxysilane (PTES). We designed an integrated device for pretreatment and paper-based detection, and successfully used for the detection of dicofol in tea. The applicability was demonstrated by detection of dicofol in real tea samples with good recovery ranging from 86% to 109%. The apparatus was convenient and could be used for on-site evaluation of dicofol.
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http://dx.doi.org/10.1016/j.foodchem.2021.131033DOI Listing
September 2021

Causes and Characteristics of Children Unintentional Injuries in Emergency Department and Its Implications for Prevention.

Front Public Health 2021 5;9:669125. Epub 2021 Aug 5.

Department of Nephrology, Children's Hospital of Fudan University, Shanghai, China.

Child unintentional injuries have become a hot topic worldwide, and substantial regional disparities existed in causes and characteristics. To date, limited data are available to investigate the causes and characteristics of child unintentional injuries from hospitals for children in China. A cross-sectional study was conducted between January 2017 and December 2018 in Shanghai, China. Patients aged <18 years with an unintentional injury presented to the emergency department were enrolled. Demographic information, Pediatric Risk for Mortality III score, and outcome variables were retrieved from electronic health records (EHRs). Frequencies and proportions of categorical variables and means and SDs of continuous variables are presented. Chi-square test and Student's -test were used for the comparison between groups, as appropriate. Logistic regression analysis was used to estimate potential risk factors for admission to the hospital. A total of 29,597 cases with unintentional injuries were identified between January 2017 and December 2018, with boys vs. girls ratio of 1.75. Preschool children account for approximately two-thirds of unintentional injuries in the emergency department. A distinctive pattern of mechanisms of unintentional injuries between gender was documented, and sports injury was significantly higher in boys than in girls (10.2 vs. 7.8%). Compared with Canadian Emergency Department Triage and Acuity Scale (CTAS) Grade 3 patients, Grade 2 [odds ratio (OR) = 2.99, 95% CI = 1.93-4.63, < 0.001] and Grade 1 (OR = 74.85, 95% CI = 12.93-433.14, < 0.001) patients had higher risk of inhospital admission. For causes of injuries, compared with falling, foreign body and poison had a lower risk of inhospital admission, while transport injury (OR = 1.31, 95% CI = 1.07-1.59, = 0.008) and high fall injury (OR = 2.58. 95% CI =1.48-4.49, < 0.001) had a significantly higher risk of admission. There was a significant relationship between age-groups and unintentional injuries between gender, with decreased injuries among girls growing up older. Preventive measures should be taken to reduce transport injury and high fall injury, which had a significantly higher risk of admission.
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http://dx.doi.org/10.3389/fpubh.2021.669125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374066PMC
October 2021

Detection of meningoencephalitis caused by Listeria monocytogenes with ischemic stroke-like onset using metagenomics next-generation sequencing: A case report.

Medicine (Baltimore) 2021 Aug;100(31):e26802

Department of Neurology, The First People's Hospital of Changde City, Changde, Hunan, China.

Rationale: Listeria monocytogenes (L. monocytogenes) is a compatible intracellular bacterial pathogen that can invade different mammalian cells and reach the central nervous system (CNS), leading to meningoencephalitis and brain abscesses. In the diagnosis of L. monocytogenes meningoencephalitis (LMM), conventional tests are often reported as negative due to antibiotic therapy or low bacterial content in cerebrospinal fluid. To date, prompt diagnosis and accurate treatment remain a challenge for patients with Listeria infections.

Patient Concerns: Here, we report a case of a 64-year-old male diagnosed with LMM by using metagenomics next-generation sequencing (mNGS).

Diagnosis: LMM was confirmed by mNGS analysis of cerebrospinal fluid.

Interventions: The patient was treated with piperacillin and sensitive antibiotics.

Outcomes: The patient could walk independently about 1 month after admission and was discharged from the hospital.

Lessons: This case highlights the value of mNGS in the diagnosis of LMM and emphasizes the inadequate sensitivity of conventional diagnostic methods for Listeria infection.
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http://dx.doi.org/10.1097/MD.0000000000026802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341303PMC
August 2021

Effects of Short-Term Dietary Fiber Intervention on Gut Microbiota in Young Healthy People.

Diabetes Metab Syndr Obes 2021 5;14:3507-3516. Epub 2021 Aug 5.

Department of Epidemiology and Biostatistics, School of Public Health, Xinjiang Medical University, Urumqi, 830001, People's Republic of China.

Gut microbiota are critical to many aspects of human health including immune and metabolic health. Long-term diet influences the community structure and activity of the trillions of microorganisms residing in the human gut, but it remains unclear how the human gut microbiome responds to short-term intervention with dietary fiber. This study explored the effects of mixed dietary fibers on gut microbiota in young, healthy people. Twelve healthy, young adults participated in a randomized, crossover trial comparing the effects of polyglucan, inulin and resistant malt dextrin on gut microbiota composition and bacterial abundances. During the study, the subjects followed their normal diets without any constraints. Microbial community profiles were determined by absolute quantification 16S rRNA gene amplicon sequencing. Mixed model analysis did not reveal an effect of dietary intervention on microbial community structure. At the genus level, dietary fiber intervention for 4 days significantly promoted the growth of and and inhibited the growth of and 2 compared with the baseline. Addition of whey albumen powder for 4 days promoted the growth of and but interfered with the growth of . Our results should be corroborated by randomized clinical trials with large sample size.
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http://dx.doi.org/10.2147/DMSO.S313385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353528PMC
August 2021

The beginning and the end: flanking nucleotides induce a parallel G-quadruplex topology.

Nucleic Acids Res 2021 Sep;49(16):9548-9559

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing210023, China.

Genomic sequences susceptible to form G-quadruplexes (G4s) are always flanked by other nucleotides, but G4 formation in vitro is generally studied with short synthetic DNA or RNA oligonucleotides, for which bases adjacent to the G4 core are often omitted. Herein, we systematically studied the effects of flanking nucleotides on structural polymorphism of 371 different oligodeoxynucleotides that adopt intramolecular G4 structures. We found out that the addition of nucleotides favors the formation of a parallel fold, defined as the 'flanking effect' in this work. This 'flanking effect' was more pronounced when nucleotides were added at the 5'-end, and depended on loop arrangement. NMR experiments and molecular dynamics simulations revealed that flanking sequences at the 5'-end abolish a strong syn-specific hydrogen bond commonly found in non-parallel conformations, thus favoring a parallel topology. These analyses pave a new way for more accurate prediction of DNA G4 folding in a physiological context.
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http://dx.doi.org/10.1093/nar/gkab681DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450091PMC
September 2021

[Design and Implementation of Medical Equipment Rational Deployment Information System Based on ASP.NET].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Jul;45(4):401-405

School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200082.

Based on ASP.NET framework, The Intelligent Estimated System for Rational Deployment of Medical Equipment (MERDIS) is designed and developed with SQL Server 2012 database and C# language. The system is used to realize the rational deployment suggestions and evaluation of medical equipment in hospitals. The system input the data of hospital medical equipment and clinical pathway into the database, and then feedback the deployment information to users which are calculated by big data information, so as to achieve the purpose of giving rational deployment of hospital medical equipment.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2021.04.011DOI Listing
July 2021

Identification of potential targets of triptolide in regulating the tumor microenvironment of stomach adenocarcinoma patients using bioinformatics.

Bioengineered 2021 12;12(1):4304-4319

School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

This study aimed to identify potential pharmacological targets of triptolide regulating the tumor microenvironment (TME) of stomach adenocarcinoma (STAD) patients. A total of 343 STAD cases from The Cancer Genome Atlas (TCGA) were assigned into high- or low-score groups applying Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE). Hub genes were identified from differentially expressed genes (DEGs) shared by stromal- and immune-related components in the TME of STAD patients using R software. Cox regression analysis was used to identify genes significantly correlated with STAD patient survival. Triptolide target genes were predicted from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Top 30 genes filtered by Cytohubba from 734 DEGs were screened as hub genes. Forty-two genes were found to be at high risk for STAD prognosis. Thirty-four targets of triptolide were predicted using the TCMSP database. Importantly, C-X-C chemokine receptor type 4 (CXCR4) was identified as a potential target of triptolide associated with the TME in STAD. Analysis of survival highlighted the association between CXCR4 upregulation with STAD progression and poor prognosis. Gene Set Enrichment Analysis (GSEA) confirmed that genes in the CXCR4- upregulated group had significant enrichment in immune-linked pathways. Additionally, triptolide targets were found to be significantly enriched in CXCR4-related chemokine and cancer-related p53 signaling pathways. Molecular docking demonstrated a high affinity between triptolide and CXCR4. In conclusion, CXCR4 may be a therapeutic target of triptolide in the treatment of STAD patients by modulating the TME.
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http://dx.doi.org/10.1080/21655979.2021.1945522DOI Listing
December 2021

Development and Implementation of a Pediatric Nursing-Clinical Decision Support System for Hyperthermia: A Pre- and Post-test.

Comput Inform Nurs 2021 Aug 3. Epub 2021 Aug 3.

Author Affiliations: Nursing Department (Dr Zhao and Gu, Mrs Hu, Mrs Wan, and Mrs Liu), Information and Technology Department (Dr Ye), and Dean's Office (Dr Zhang), Children's Hospital of Fudan University, Shanghai, People's Republic of China.

This article describes the development process and application of the Pediatric Nursing-Clinical Decision Support System for Hyperthermia. Firstly, we formed the Pediatric Nursing-Knowledge Base for Hyperthermia, which combines publicly available clinical practice guidelines and nursing routines of hyperthermia management. Then, following the nursing process framework, the system was developed using clinical decision support technology. Finally, a pre- and post-test were adopted to examine the effectiveness, usability, and feasibility before (1st to 31st of August 2018) and after (1st to 31st of December 2019) using the system. Its effectiveness was examined by analysis of nursing records' quality, including completeness of nursing assessment, timeliness of nursing diagnosis, individualization of nursing interventions, and timeliness of nursing evaluation. Its usability and feasibility were assessed using the Clinical Nursing Information System Effectiveness Evaluation Scale. There was a significant difference between the two groups in effectiveness, usability, and feasibility. Although the system was developed specifically for our hospital workflow and processes, the Pediatric Nursing-Knowledge Base for Hyperthermia and workflow for hyperthermia management in this study can be used as a reference to other hospitals.
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http://dx.doi.org/10.1097/CIN.0000000000000812DOI Listing
August 2021

Screening-based identification of xanthone as a novel NLRP3 inflammasome inhibitor via metabolic reprogramming.

Clin Transl Med 2021 07;11(7):e496

Eye Center of the 2nd Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China.

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http://dx.doi.org/10.1002/ctm2.496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8288006PMC
July 2021

A Novel Allele Encoding 7-Hydroxymethyl Chlorophyll a Reductase Confers Bacterial Blight Resistance in Rice.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China.

Rice spotted leaf mutants are helpful to investigate programmed cell death (PCD) and defense response pathways in plants. Using a map-based cloning strategy, we characterized novel rice spotted leaf mutation that encodes a 7-hydroxymethyl chlorophyll a reductase (). The wild-type (WT) allele could rescue the mutant phenotype, as evidenced by complementation analysis. was constitutively expressed at all rice tissues tested and its expression products localized to chloroplasts. The mutant exhibited PCD and leaf senescence with increased HO (hydrogen peroxide) accumulation, increased of ROS (reactive oxygen species) scavenging enzymes activities and TUNEL (terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling) -positive nuclei, upregulation of PCD related genes, decreased chlorophyll (Chl) contents, downregulation of photosynthesis-related genes, and upregulation of senescence-associated genes. Besides, the mutant exhibited enhanced bacterial blight resistance with significant upregulation of defense response genes. Knockout lines of exhibited spotted leaf phenotype, cell death, leaf senescence, and showed increased resistance to the bacterial pathogen pv.   coupled with upregulation of five pathogenesis-related marker genes. The overexpression of resulted in increased susceptibility to with decreased expression of pathogenesis-related marker genes. Altogether, our findings revealed that is involved in regulating cell death and defense response against bacterial blight pathogen in rice.
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http://dx.doi.org/10.3390/ijms22147585DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303675PMC
July 2021

miR‑29a‑3p regulates the epithelial‑mesenchymal transition via the SPARC/ERK signaling pathway in human bronchial epithelial cells.

Int J Mol Med 2021 Sep 19;48(3). Epub 2021 Jul 19.

Pediatric Department, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Neutrophilic asthma (NA) is a subtype of asthma that responds poorly to corticosteroid treatment. In certain diseases, microRNA (miR)‑29a‑3p is considered to be a key regulatory molecule for remodeling of the extracellular matrix. However, the effect of miR‑29a‑3p on airway remodeling is unknown. The present study aimed to investigate the role of miR‑29a‑3p in NA. A mouse model of NA was established and these animals were compared to normal controls. Both groups of mice were subjected to lung function tests and histopathological analysis. Human bronchial epithelial cells (16HBE) were grown in culture and incubated with secreted protein acidic rich in cysteine (SPARC) and a miR‑29a‑3p mimic. The expression of miR‑29a‑3p, SPARC and epithelial‑mesenchymal transition (EMT)‑related markers were measured using reverse transcription‑quantitative PCR and western blotting. Luciferase reporter assay was performed to identify the direct regulatory relationship between miR‑29a‑3p and SPARC. miR‑29a‑3p expression was significantly decreased, while SPARC expression was increased in the NA mouse model with a phenotype of EMT. Overexpression of SPARC downregulated the expression of E‑cadherin, while it increased the expression of vimentin in 16HBE cells. miR‑29a‑3p administration reversed the SPARC‑induced effects on E‑cadherin and vimentin expression. Luciferase assays confirmed that SPARC was the target gene for miR‑29a‑3p. Furthermore, SPARC overexpression increased the protein expression of phosphorylated (p)‑ERK, while transfection with miR‑29a‑3p mimics significantly inhibited this increase. The data suggested that EMT in the NA mouse model was associated with decreased levels of miR‑29a‑3p and elevated SPARC. Furthermore, SPARC could induce the formation of EMT in 16HBE cells in vitro and this was directly targeted by miR‑29a‑3p and mediated by p‑ERK, suggesting that miR‑29a‑3p may participate in the airway remodeling of NA.
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http://dx.doi.org/10.3892/ijmm.2021.5004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285050PMC
September 2021

Transformable upconversion metal-organic frameworks for near-infrared light-programmed chemotherapy.

Chem Commun (Camb) 2021 Aug;57(63):7826-7829

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

A transformable upconversion MOF comprising a UCNP core and an azobenzene-based MOF shell is designed for NIR light-modulated chemotherapy. The dual Förster resonance energy transfers (FRETs) involved in this delivery system trigger the transformation of the MOF for drug release and prodrug activation, thus significantly inhibiting the tumor growth and metastasis.
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http://dx.doi.org/10.1039/d1cc02670dDOI Listing
August 2021

Associations of fine particulate matter and constituents with pediatric emergency room visits for respiratory diseases in Shanghai, China.

Int J Hyg Environ Health 2021 07 13;236:113805. Epub 2021 Jul 13.

School of Public Health, Key Lab of Public Health Safety of the Ministry of Education and NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China. Electronic address:

Background: Although ambient fine particulate matter (PM) has been associated with adverse respiratory outcomes in children, few studies have examined PM constituents with respiratory diseases in children in China.

Objectives: To investigate the associations of short-term exposure to PM and its constituents with pediatric emergency room visits (ERVs) for respiratory diseases in Shanghai, China.

Methods: We collected daily concentrations of PM and its constituents in urban Shanghai from January 1, 2016, to December 31, 2018. Daily pediatric ERVs for four major respiratory diseases, including upper respiratory tract infection, bronchitis, pneumonia, and asthma, were obtained from 66 hospitals in Shanghai during the same period. Associations of exposure to daily PM and constituents with respiratory ERVs were estimated using the over-dispersed generalized additive models.

Result: Short-term exposure to PM and its constituents were associated with increased pediatric ERVs for respiratory diseases. Specifically, an interquartile range increase in the 3-day average PM level (31 μg/m) was associated with 1.86% (95%CI: 0.52, 3.22), 1.53% (95%CI: 0.01, 3.08), 1.90% (95%CI: 0.30, 3.52), and 2.67% (95%CI: 0.70, 4.68) increase of upper respiratory tract infection, bronchitis, pneumonia, and asthma ERVs, respectively. As for PM constituents, we found organic carbon, ammonium, nitrate, selenium, and zinc were associated with higher risk of respiratory ERVs in the single constituent and the constituent-PM models.

Conclusion: Short-term exposure to PM was associated with increased pediatric ERVs for respiratory diseases. Constituents related to anthropogenic combustion and traffic might be the dominant contributors of the observed associations.
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http://dx.doi.org/10.1016/j.ijheh.2021.113805DOI Listing
July 2021

Detection of Chlamydia psittaci in both blood and bronchoalveolar lavage fluid using metagenomic next-generation sequencing: A case report.

Medicine (Baltimore) 2021 Jul;100(27):e26514

Department of Critical Care Medicine.

Rationale: Chlamydia psittaci (C psittaci) is a gram-negative obligate intracellular parasite, with birds as main hosts. The main route of infection in humans is inhalation of aerosols from contaminated animal excreta through the respiratory tract. The main manifestation of C psittaci infection is pneumonia. Patients suffering from severe infection are prone to sepsis and multiple organ failure. We report a case of simultaneous detection of C psittaci in blood and bronchoalveolar lavage fluid using metagenomic next-generation sequencing (mNGS) technology.

Patient Concerns: The 71-year-old male patient was a farmer with a long history of raising poultry and initial symptoms of fever and muscle pain accompanied by limb weakness and paroxysmal cough.

Diagnoses: The patient was diagnosed with sepsis, severe pneumonia, and multiple organ failure.

Interventions: Anti-infective therapy with doxycycline and meropenem was applied.

Outcomes: The patient's body temperature and infection indicators improved and the chest X-ray findings showed the amelioration of lesions after 18 days of treatment. The patient was discharged without treatment on hospital day 19 due to financial constraints and subsequently died after 7 days.

Lessons: mNGS is an excellent diagnostic tool when specific pathogens are undetected by traditional assays.
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http://dx.doi.org/10.1097/MD.0000000000026514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270604PMC
July 2021

Development trajectory for the temporal and spatial evolution of the resilience of regional tourism environmental systems in 14 cities of Gansu Province, China.

Environ Sci Pollut Res Int 2021 Jul 6. Epub 2021 Jul 6.

Department of Economy and Management, Lanzhou University of Technology, Lanzhou, 730050, China.

The rapid development of the urban economy in China and the accompanying income growth experienced by urban residents have increased demand for tourism and leisure, which has brought pressure on the urban tourism environment system (UTES), making the contradiction between tourism economic development and the ecological environment increasingly acute. While seeking to rationalize the economic, social, and ecological benefits of tourism, reducing the fragility of the UTES and improving its anti-interference and recovery capabilities have become attracted significant attention from scholars in China and elsewhere. This paper establishes a definition of resilience for an UTES and constructs an evaluation index system for it in terms of the social, economic, and ecological environments. It also establishes an entropy weight-TOPSIS resilience evaluation model to measure resilience in regional systems, using ArcGIS to analyze the standard deviation ellipse and center of the gravity track of the resilience. System dynamics was used to construct diagrams of causal relationships and stock flow for the constituent elements of UTES to show the mechanisms that promote its resilience. This paper investigates 14 cities of Gansu Province in particular to simulate the resilience model of a regional system.
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http://dx.doi.org/10.1007/s11356-021-14932-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260156PMC
July 2021

Correction to: PREMATURE SENESCENCE LEAF 50 Promotes Heat Stress Tolerance in Rice (Oryza sativa L.).

Rice (N Y) 2021 Jul 5;14(1):63. Epub 2021 Jul 5.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.

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http://dx.doi.org/10.1186/s12284-021-00506-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8257812PMC
July 2021

Corrigendum to "Construction of Protein-related Risk Score Model in Bladder Urothelial Carcinoma".

Biomed Res Int 2021 2;2021:9758361. Epub 2021 Jun 2.

Xiangya International Medical Center, Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, China.

[This corrects the article DOI: 10.1155/2020/7147824.].
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http://dx.doi.org/10.1155/2021/9758361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203343PMC
June 2021

Self-assembled micelle responsive to quick NIR light irradiation for fast drug release and highly efficient cancer therapy.

J Control Release 2021 Aug 23;336:469-479. Epub 2021 Jun 23.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address:

Upconversion nanoparticles (UCNPs) have been used for designing near infrared (NIR) light-responsive nanocarriers and controllable drug release. However, the need for long-term NIR light irradiation over hours impaired their application efficiency. Here we develop a self-assembled micelle of amphipathic polymer P-DASA which degrades via quick NIR light irradiation. UCNPs and DOX are also encapsulated in the micelle for quick drug release. P-DASA is composed of hydrophilic polyethylene glycol segment and photo-responsive hydrophobic donor-acceptor Stenhouse adduct (DASA). Only 5-min NIR irradiation causes the hydrophilicity conversion of P-DASA and the complete disruption of micelle with DOX fast release of 83.7% in 30 min to achieve highly efficient therapy. Moreover, the P-glycoprotein mediated DOX efflux is also diminished by concomitantly producing NO intracellularly. This micelle demonstrates impressive in vivo therapeutic effect, and thus provides an avenue for highly efficient cancer therapy.
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http://dx.doi.org/10.1016/j.jconrel.2021.06.028DOI Listing
August 2021

Significance of serum and pathological biomarkers in fertility-sparing treatment for endometrial cancer or atypical hyperplasia: a retrospective cohort study.

BMC Womens Health 2021 06 23;21(1):252. Epub 2021 Jun 23.

Department of Obstetrics and Gynecology, Peking University People's Hospital, 11th Xizhimen South Street, Xicheng District, Beijing, 100044, China.

Background: This study analyzed the changes of serum and pathological biomarkers during fertility-sparing therapy of endometrial cancer (EC) or endometrial atypical hyperplasia (EAH), to investigate their implications for early prediction of treatment efficacy.

Methods: A retrospective analysis of EC or EAH patients who received fertility-sparing therapy between 2012 and 2016 was performed. Serum and endometrium sampling were obtained for each patient at three time points: at baseline, at 3-6 months' treatment and at the end of conservative treatment. Serum biomarkers including insulin resistance (HbA1c, HOMA-IR), sex hormones and thyroid hormones were measured. Meanwhile expression of endometrial pathological biomarkers including ER, PR, PRB and Ki-67 was also assessed by immunohistochemistry.

Results: For the 53 recruited patients, overall complete response, recurrence and pregnancy rates were 94%, 26% and 36.4%. During the treatment, the serum biomarkers of HOMA-IR remained stable, while pathological markers including PR, PRB and Ki67 diminished significantly. Patients who achieved remission faster had significant lower HOMA-IR level and higher PRB expression at baseline. We also found a more remarkable down-regulation of PRB related with faster remission. Further multivariate analysis confirmed that baseline HOMA-IR ≥ 2.5 negatively affected treatment time to remission (OR 0.206; p = 0.017). While marked reduction of PRB (≥ 30%) at 3-6 months' treatment correlated with faster remission (OR 5.788; p = 0.010).

Conclusion: For EC and EAH patients who received fertility-sparing therapy, baseline status of insulin resistance predicted poor response to progestin, while marked reduction of PRB following the initial 3-6 months' treatment predicted fast remission.
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http://dx.doi.org/10.1186/s12905-021-01383-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223344PMC
June 2021

Primitive neuroectodermal tumor of the pericardium: a case report and literature review.

BMC Cardiovasc Disord 2021 06 16;21(1):305. Epub 2021 Jun 16.

Department of Radiology, The First Medical Center of Chinese, PLA General Hospital, No.28 Fuxing Street of Haidian District, Beijing, 100853, China.

Background: The primitive neuroectodermal tumors (PNETs) are a family of highly malignant tumors with a multidirectional differential potential. The tumors are characterized by aggressive small round tumor cells that originate from the spinal cord of the central and sympathetic nervous systems. Cases involving the pericardium are extremely rare. Herein, we present a case of peripheral primitive neuroectodermal tumor (pPNET) that originated in the pericardium.

Case Presentation: A 23-year-old woman presented with cough and progressive dyspnea for 1 month, followed by eyelid and facial edema for 10 days, without any apparent cause. Significantly elevated tumor markers were detected in her blood. A cardiac ultrasound revealed a 74 mm × 61 mm spherical mass that was attached to the left pericardium, as well as massive pericardial effusion. Positron emission tomography-CT (PET-CT) showed focal hypermetabolism in the left pericardium. Via histopathology and immunohistochemistry, the spherical mass was identified as PNETS. The patient was successfully treated with a combination of surgical resection via thoracotomy and postoperative chemotherapy, and she was disease-free for 7 years at follow-up. Unfortunately, at 7 years after the treatment, the patient's pPNET recurred. Positron emission tomography-MRI (PET-MRI) and 64-slice coronary CTA revealed that the aorta and multiple coronary arteries were involved. Subsequently, the patient refused a heart transplant and voluntarily left the hospital.

Conclusions: This paper reports on a rare and recurrent case of PNET in the parietal pericardium. With respect to the different biologic characteristics and prognoses of pPNETs (compared to other known pericardium tumors), it is essential to consider this entity as a differential diagnosis in pericardium tumors.
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http://dx.doi.org/10.1186/s12872-021-02113-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207573PMC
June 2021

A Targeted Erythrocyte Membrane-Encapsulated Drug-Delivery System with Anti-osteosarcoma and Anti-osteolytic Effects.

ACS Appl Mater Interfaces 2021 Jun 14;13(24):27920-27933. Epub 2021 Jun 14.

Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, Hunan, China.

Chemotherapy is one of the main treatment methods for osteosarcoma. However, conventional chemotherapy lacks targeting properties, and its long-term and extensive use will have serious side effects on patients. For this reason, a multifunctional nanodrug system (V-RZCD) targeting osteosarcoma was developed in this study. V-RZCD consists of two parts: (1) the core (ZCD), wherein calcium ions (Ca) and zoledronic acid (ZA) form a metal-organic framework for loading doxorubicin (DOX), and (2) the shell (V-R), a vascular endothelial growth factor (VEGF) ligand-modified red blood cell membrane nanovesicle. By targeting the VEGF, V-RZCD can specifically bind to the VEGF receptors that are highly expressed on the surface of osteosarcoma cells. Importantly, compared with free ZA and DOX, V-RZCD not only clearly inhibits the proliferation of osteosarcoma but also significantly inhibits osteolysis induced by osteosarcoma. In summary, V-RZCD represents a new way to treat osteosarcoma.
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http://dx.doi.org/10.1021/acsami.1c06059DOI Listing
June 2021

PREMATURE SENESCENCE LEAF 50 Promotes Heat Stress Tolerance in Rice (Oryza sativa L.).

Rice (N Y) 2021 Jun 12;14(1):53. Epub 2021 Jun 12.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.

Background: Heat stress is a major environmental factor that could induce premature leaf senescence in plants. So far, a few rice premature senescent leaf mutants have been reported to involve in heat tolerance.

Findings: We identified a premature senescence leaf 50 (psl50) mutant that exhibited a higher heat susceptibility with decreased survival rate, over-accumulated hydrogen peroxide (HO) content and increased cell death under heat stress compared with the wild-type. The causal gene PREMATURE SENESCENCE LEAF 50 (PSL50) was isolated by using initial map-based resequencing (IMBR) approach, and we found that PSL50 promoted heat tolerance probably by acting as a modulator of HO signaling in response to heat stress in rice (Oryza sativa L.).

Conclusions: PSL50 negatively regulates heat-induced premature leaf senescence in rice.
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http://dx.doi.org/10.1186/s12284-021-00493-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197683PMC
June 2021
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