Publications by authors named "Xiaobo Chen"

239 Publications

The utility of serum inhibin B, anti-Müllerian hormone and insulin growth factor-1 in predicting a positive response to GnRH analogs for diagnosing central precocious puberty in girls.

J Pediatr Endocrinol Metab 2021 Jul 21. Epub 2021 Jul 21.

Peking Union Medical College Graduate School, Beijing, P. R. China.

Objectives: The use of inhibin B (INHB), anti-Müllerian hormone (AMH) and insulin-like growth factor-1 (IGF-1) in differentiating central precocious puberty (CPP) from non-CPP was evaluated.

Methods: In total, 115 Chinese girls were recruited (CPP: 44, non-CPP: 71). The diagnostic performance of INHB, AMH and IGF-1 in differentiating CPP from non-CPP was analyzed using receiver operating characteristic (ROC) curves.

Results: INHB levels were higher in the CPP group than in the non-CPP group (55.56 ± 22.42 vs. 32.97 ± 15.59 pg/mL; p<0.001). AMH levels were similar in the CPP and non-CPP groups (6.63 ± 3.74 vs. 5.70 ± 3.15 pg/mL; p=0.158), and IGF-1 levels were much higher in the CPP group than in the non-CPP group (290.75 ± 79.78 vs. 200.10 ± 54.01 pg/mL; p<0.001). The area under the ROC curve (AUC) was greatest for INHB (0.819, standard error (SE) 0.041), followed by IGF-1 (0.809, SE 0.047) and AMH (0.567, SE 0.057). Among the ROC curves including combinations of these parameters, the AUC for INHB + IGF-1 was 0.849 and that for INHB + AMH was 0.768.

Conclusions: Serum INHB and IGF-1 measurements could predict positive responses to gonadotropin-releasing hormone (GnRH) analog stimulation in girls with precocious puberty.
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http://dx.doi.org/10.1515/jpem-2021-0137DOI Listing
July 2021

Constructing high-order functional connectivity network based on central moment features for diagnosis of autism spectrum disorder.

PeerJ 2021 6;9:e11692. Epub 2021 Jul 6.

School of Computer Science and Technology, Shandong Technology and Business University, Yantai, Shandong, China.

The sliding-window-based dynamic functional connectivity network (D-FCN) has been becoming an increasingly useful tool for understanding the changes of brain connectivity patterns and the association of neurological diseases with these dynamic variations. However, conventional D-FCN is essentially low-order network, which only reflects the pairwise interaction pattern between brain regions and thus overlooking the high-order interactions among multiple brain regions. In addition, D-FCN is innate with temporal sensitivity issue, i.e., D-FCN is sensitive to the chronological order of its subnetworks. To deal with the above issues, we propose a novel high-order functional connectivity network framework based on the central moment feature of D-FCN. Specifically, we firstly adopt a central moment approach to extract multiple central moment feature matrices from D-FCN. Furthermore, we regard the matrices as the profiles to build multiple high-order functional connectivity networks which further capture the higher level and more complex interaction relationships among multiple brain regions. Finally, we use the voting strategy to combine the high-order networks with D-FCN for autism spectrum disorder diagnosis. Experimental results show that the combination of multiple functional connectivity networks achieves accuracy of 88.06%, and the best single network achieves accuracy of 79.5%.
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http://dx.doi.org/10.7717/peerj.11692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269664PMC
July 2021

A 12-Year-Old Girl Presenting With Recurrent Dyspnea and Pulmonary Ground-Glass Opacities.

Chest 2021 Jul;160(1):e45-e50

Department of Critical Care and Respiratory Medicine, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangdong, China. Electronic address:

Case Presentation: A 12-year-old girl presented with shortness of breath with exercise for 2 weeks. Her oxygen saturation was 85% during exercise. Birth and family histories were unremarkable. The girl was healthy until 7.1 years of age, when she suffered a "pneumonia" with fever, dyspnea, and hypoxemia, which diminished after a 19-day treatment with antibiotics and methylprednisolone. These symptoms relapsed 8 months later, and she was diagnosed with rapidly progressive interstitial lung disease (ILD) and a Mycoplasma pneumoniae infection. At that time, her symptoms failed to respond to a course of antibiotic therapy but resolved with IV methylprednisolone at 2.7 mg/kg/day. She remained on a tapering dose of methylprednisolone plus methotrexate for the next 18 months until withdrawal of these medications because of return of almost normal lung imaging. She had never had myalgia, muscle weakness, arthritis, rashes, mechanic's hands, Raynaud's phenomenon, dry mouth, or dry eyes.
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http://dx.doi.org/10.1016/j.chest.2021.02.002DOI Listing
July 2021

The Application of Transbronchial Lung Cryobiopsy and Uniportal and Tubeless Video-Assisted Thoracic Surgery in the Multidisciplinary Diagnosis of Interstitial Lung disease-A Real-World Prospective Study.

Front Mol Biosci 2021 16;8:681669. Epub 2021 Jun 16.

Department of Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

The application of transbronchial lung cryobiopsy (TBLC) and uniportal and tubeless video-assisted thoracic surgery (UT-VATS) in the multidisciplinary diagnosis of interstitial lung disease (ILD) has not been demonstrated in real-world clinical practice. This prospective study included 137 patients with no definitive diagnosis who were the subject of two multidisciplinary discussion (MDD) sessions. As indicated in the first MDD, 67 patients underwent UT-VATS and 70 underwent TBLC. The specificity of biopsy information and its contribution to final MDD diagnosis were evaluated in the second MDD. The post-operative complications and hospitalization costs associated with the two biopsy methods were compared. UT-VATS was favored for patients initially diagnosed with idiopathic pulmonary fibrosis (IPF), bronchiolitis-associated interstitial lung disease (RB-ILD)/desquamative interstitial pneumonia (DIP) and undefined idiopathic interstitial pneumonia (UIIP), while TBLC was preferred for pulmonary lymphangioleiomyomatosis (PLAM) and pulmonary alveolar proteinosis (PAP). The spirometry parameters were better in patients who underwent UT-VATS than those who underwent TBLC. UT-VATS provided more specific pathological results than TBLC (85.7 vs 73.7%, = 0.06). In patients initially diagnosed with UIIP, pathological information from UT-VATS was more clinically useful than that obtained from TBLC, although both tests contributed similarly to cases initially diagnosed as interstitial pneumonia with auto-immune features (IPAF)/connective tissue disease-related ILD (CTD-ILD). The safety of UT-VATS was comparable with TBLC although TBLC was cheaper during hospitalization (US$4,855.7 vs US$3,590.9, < 0.001). multidisciplinary discussion decisions about biopsies were driven by current knowledge of sampling and diagnosis capacity as well as potential risks of different biopsy methods. The current MDD considered UT-VATS more informative than TBLC in cases initially diagnosed as UIIP although they were equally valuable in patients initially diagnosed with IPAF/CTD-ILD.
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http://dx.doi.org/10.3389/fmolb.2021.681669DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241905PMC
June 2021

Clinical applications of mesenchymal stromal cell-based therapies for pulmonary diseases: An Update and Concise Review.

Int J Med Sci 2021 1;18(13):2849-2870. Epub 2021 Jun 1.

Department of Clinical Laboratory Medicine, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.

Lung disorders are a leading cause of morbidity and death worldwide. For many disease conditions, no effective and curative treatment options are available. Mesenchymal stromal cell (MSC)-based therapy is one of the cutting-edge topics in medical research today. It offers a novel and promising therapeutic option for various acute and chronic lung diseases due to its potent and broad-ranging immunomodulatory activities, bacterial clearance, tissue regeneration, and proangiogenic and antifibrotic properties, which rely on both cell-to-cell contact and paracrine mechanisms. This review covers the sources and therapeutic potential of MSCs. In particular, a total of 110 MSC-based clinical applications, either completed clinical trials with safety and early efficacy results reported or ongoing worldwide clinical trials of pulmonary diseases, are systematically summarized following preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, including acute/viral pulmonary disease, community-acquired pneumonia (CAP), chronic obstructive pulmonary disease (COPD), bronchopulmonary dysplasia (BPD), interstitial lung diseases (ILD), chronic pulmonary fibrosis, bronchiolitis obliterans syndrome (BOS) and lung cancer. The results of recent clinical studies suggest that MSCs are a promising therapeutic approach for the treatment of lung diseases. Nevertheless, large-scale clinical trials and evaluation of long-term effects are necessary in further studies.
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http://dx.doi.org/10.7150/ijms.59218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8241779PMC
June 2021

A Multicenter Survey of Type I Diabetes Mellitus in Chinese Children.

Front Endocrinol (Lausanne) 2021 15;12:583114. Epub 2021 Jun 15.

Department of Endocrinology, Hunan Provincial Children's Hospital, Changsha, China.

Purpose: To investigate the features and treatment status of children with type 1 diabetes mellitus (T1DM) in China.

Methods: We recruited patients <14 years of age with T1DM from 33 medical centers in 25 major cities of China between January 2012 and March 2015. All patients completed a questionnaire that was conducted by their pediatric endocrinologists at all centers.

Results: A total of 1,603 children (755 males and 848 females) with T1DM participated in this survey. Of these, 834 (52.03%) of the patients exhibited diabetic ketoacidosis (DKA) at onset, while 769 patients (47.97%) did not exhibit DKA (non-DKA) at onset. There was a higher proportion of females (55.71%) in the cohort of patients exhibiting DKA at onset than in the non-DKA cohort (49.33%). The mean age of patients exhibiting DKA at presentation was 7.12 ± 0.14 years; this was significantly younger than that in non-DKA group (7.79 ± 0.15 years; P < 0.005). The frequency of DKA in 3 years old, 3-7 years old, and 7 years old or more was 77.21%, 26.17%, and 37.62%, respectively. Upon initial diagnosis, 29.4%, 15.2% and 11.8% of patients showed positivity for glutamic acid decarboxylase antibody (GADA), Insulin autoantibodies (IAA), or islet cell antibody (ICA), respectively. During six months follow-up, 244 patients (15.21%) reported receiving insulin pump therapy, and more than 60% of patients monitored their blood glucose levels less than 35 times per week. Although the majority of patients had no problems with obtaining insulin, 4.74% of the children surveyed were not able to receive insulin due to financial reasons, a shortage of insulin preparations, or the failure of the parents or guardians to acquire the appropriate medicine.

Conclusion: DKA is more common in very young children. Treatment and follow-up of T1DM in China still face very serious challenges.
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http://dx.doi.org/10.3389/fendo.2021.583114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8239384PMC
June 2021

Tunable Pore Size from Sub-Nanometer to a Few Nanometers in Large-Area Graphene Nanoporous Atomically Thin Membranes.

ACS Appl Mater Interfaces 2021 Jun 16. Epub 2021 Jun 16.

Institute of Microelectronics, School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China.

Membranes are key components in chemical purification, biological separation, and water desalination. Traditional polymeric membranes are subjected to a ubiquitous trade-off between permeance and selectivity, which significantly hinders the separation performance. Nanoporous atomically thin membranes (NATMs), such as graphene NATMs, have the potential to break this trade-off. Owing to their uniqueness of two-dimensional structure and potential nanopore structure controllability, NATMs are expected to have outstanding selectivity through molecular sieving while achieving ultimate permeance at the same time. However, a drastic selectivity discrepancy exists between the proof-of-concept demonstrations and scalable separation applications in graphene membranes. In this paper, we offer a possible solution to narrow this discrepancy by tuning the pore density and pore size separately with two successive plasma treatments. We demonstrate that by narrowing the pore size distribution, the selectivity of graphene membranes can be greatly increased. Low-energy argon plasma is first applied to nucleate high density of defects in graphene. Controlled oxygen plasma is then utilized to selectively enlarge the defects into nanopores with desired sizes. This method is scalable, and the fabricated 1 cm graphene NATMs with sub-nanometer pores can separate KCl and Allura Red with a selectivity of 104 and a permeance of 1.1 × 10 m s. The pores in NATMs can be further tuned from gas-selective sub-nanometer pores to a few nanometer size. The fabricated NATMs show a selectivity of 35 between CO and N. With longer enlargement time, a selectivity of 21.2 between a lysozyme and bovine serum albumin can also be achieved with roughly four times higher permeance than that of a commercial dialysis membrane. This research offers a solution to realize NATMs of tunable pore size with a narrow pore size distribution for different separation processes from sub-nanometer in gas separation or desalination to a few nanometers in dialysis.
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http://dx.doi.org/10.1021/acsami.1c06243DOI Listing
June 2021

[Analysis of clinical features and genetic variants among 12 children with Gitelman syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):526-530

Department of Endocrinology, Children's Hospital, Capital Institute of Pediatrics, Beijing 100020, China.

Objective: To summarize clinical manifestations and results of genetic testing in 12 children with Gitelman syndrome (GS).

Methods: Clinical data of the children was collected. Whole exome sequencing(WES) was carried out to screen potential variants of genomic DNA. Candidate variants were verified by Sanger sequencing.

Results: The patients have included 10 boys and 2 girls, whom were diagnosed at between 2.8 to 15.0 year old. Six patients were due to infections, 5 were due to short stature, and 1 was due to lower limb weakness. All patients were found to carry variants of SLC12A3 gene, which included 11 with compound heterozygous variants and 1 with homozygous variant. All of the 19 alleles of the SLC12A3 gene carried by the patients were delineated, which included 15 missense variants, 2 frameshift variants and 2 splice region variants. These variants were unreported previously, which included c.578_582dupCCACC (p.Asn195Profs*109), c.251C>T (p.Pro84Leu) and c.2843G>A (p.Trp948X).

Conclusion: The clinical symptoms of GS in children are atypical and often seen in older children. For children with occasional hypokalemia associated with growth failure, GS should be suspected. The majority of GS children carry two pathogenic variants of the SLC12A3 gene, mainly compound heterozygotes, among which p.Thr60Met is the most common one. The discovery of new variants has enriched the spectrum of SLC12A3 gene variants.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200224-00096DOI Listing
June 2021

Mesoporogen-Free Strategy to Construct Hierarchical TS-1 in a Highly Concentrated System for Gas-Phase Propene Epoxidation with H and O.

ACS Appl Mater Interfaces 2021 Jun 26;13(22):26134-26142. Epub 2021 May 26.

State Key Lab of Heavy Oil Processing, China University of Petroleum, Qingdao 266580, China.

Hierarchical TS-1 has attracted enormous attention from both academia and industry due to its remarkable catalytic performance in epoxidation reactions. However, sustainable synthesis of hierarchical-nanosized TS-1 without mesoporogens is still challenging. In this work, we report a facile and mesoporogen-free strategy to simultaneously manipulate pore structure and particle size of TS-1 employing the concentrated system. Taking advantage of the suspended nuclei in the concentrated system as confirmed by the DLS-PSD and atomic force microscopy, the novel TS-1 is demonstrated to have higher Ti concentration on surface, higher surface area (539 m/g), abundant mesopores, and reduced crystal size (ca. 150 nm). Moreover, this Au-Ti bifunctional catalyst shows a good PO formation rate with enhanced catalytic stability due to the hierarchical structure. This strategy opens a novel way for the green synthesis of hierarchical-nanosized TS-1 and facilitates industrial development of the Au/TS-1 catalyst for propene epoxidation.
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http://dx.doi.org/10.1021/acsami.1c06964DOI Listing
June 2021

Precocious Puberty in Boys: A Study Based on 5 Years of Data From a Single Center in Northern China.

J Clin Res Pediatr Endocrinol 2021 May 20. Epub 2021 May 20.

Department of Endocrinology, Children's Hospital Capital Institute of Pediatrics, Beijing, China.

Aims: To evaluate the clinical features and etiology of precocious puberty in Chinese boys.

Methods: In this study, data from boys who were referred for the evaluation of precocious puberty from 2015 to 2020 at a tertiary hospital in Northern China were retrospectively analyzed.

Results: Eighty-two boys were diagnosed with precocious puberty (PP) from 2015 to 2020. Sixty-two patients (75.6%) were diagnosed with central precocious puberty (CPP), and twenty patients (24.4%) were diagnosed with peripheral PP (PPP). In the CPP group, forty-nine cases were classified as idiopathic CPP (ICPP), and thirteen patients had pathogenic CPP. The top three causes of PPP were congenital adrenal hyperplasia (CAH), germ cell tumors (GCTs) secreting human chorionic gonadotropin (hCG) and familial male-limited precocious puberty (FMPP).

Conclusions: The etiology of precocious puberty in males is diverse. The majority of CPP cases in Chinese boys are idiopathic rather than organic.
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http://dx.doi.org/10.4274/jcrpe.galenos.2021.2021.0033DOI Listing
May 2021

Applied pressure of cooling gas: a neglected determinant for size of specimens in transbronchial cryobiopsy.

J Thorac Dis 2021 Apr;13(4):2099-2105

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Transbronchial cryobiopsy (TBCB) is an option to surgical biopsy for the diagnosis in interstitial lung diseases. Several impact factors have received wide attention, including the freezing time, cryoprobe size, and contact pressure. However, the effect of the applied gas pressure on the specimen size has not been well elucidated. The purpose of this study is to investigate the effect of the applied gas pressure on the TBCB specimen size.

Methods: Cryoprobes with a diameter of 1.9 mm were used to perform TBCB on 4 beagle canines under general anesthesia. TBCB was performed with a total of 16 time-pressure combinations that were randomly combined with 4 freezing times (3, 4, 5, and 6 s) and 4 gas pressures (40, 50, 55, and 60 bar). For each combination, 8 biopsies were performed. The size and quality of specimens, as well as complications, were evaluated.

Results: A total of 128 TBCB specimens were obtained. With the same freezing time, the specimen sizes obtained by different applied gas pressures were significantly different (P<0.05) and positively correlated with the gas pressures (r: 0.797-0.867). With the same gas pressure, the size of the TBCB specimens was positively correlated with the freezing time (r: 0.503-0.752). In the 40-bar group, no tissues were obtained when the freezing times were 3-5 s. In the 50-bar and 55-bar groups, qualified specimens were obtained when the freezing times were 5 and 6 s. In the 60-bar group, qualified specimens were obtained when the freezing times were 3-6 s.

Conclusions: The TBCB specimen size was positively correlated with the applied gas pressure. The applied gas pressure contributed to the sample size and quality. To obtain qualified specimens with a 1.9-mm cryoprobe during TBCB, the lowest limit of the normal working gas pressure range should be increased to greater than 50 bar.
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http://dx.doi.org/10.21037/jtd-20-2446DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107553PMC
April 2021

Value of anal swabs for SARS-COV-2 detection: a literature review.

Int J Med Sci 2021 16;18(11):2389-2393. Epub 2021 Apr 16.

Department of rheumatology and immunology, Tianjin medical university general hospital, Tianjin, China.

Facing the unprecedented global public health crisis caused by coronavirus disease 2019 (COVID-19), nucleic acid tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are the gold standard for diagnosing COVID-19. The asymptomatic carriers were not suspected of playing a significant role in the ongoing pandemic, and universal nucleic acid screening in close contacts of confirmed cases and asymptomatic carriers has been carried out in many medium- and high-risk areas for the spread of the virus. Recently, anal swabs for key population screening have been shown to not only reduce missed diagnoses but also facilitate the traceability of infectious sources. As a specimen for the detection of viruses, the goal of this paper is to briefly review the transmission route of SARS-CoV-2 and the necessity of using anal swabs for SARS-CoV-2 screening to minimize transmission and a threat to other people with COVID-19.
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http://dx.doi.org/10.7150/ijms.59382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100643PMC
May 2021

Perspectives on anti-IL-1 inhibitors as potential therapeutic interventions for severe COVID-19.

Cytokine 2021 07 17;143:155544. Epub 2021 Apr 17.

The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China. Electronic address:

The overproduction of proinflammatory cytokines, resulting in what has been described as a cytokine storm or cytokine release syndrome (CRS), may be the key factor in the pathology of severe coronavirus disease 2019 (COVID-19) and is also a crucial cause of death from COVID-19. With the purpose of finding effective and low-toxicity drugs to mitigate CRS, IL-1 blockade agents, which are one of the safest ways to stop this overwhelming innate immune response, are already available in several preliminary reports or are under observational trials and may offer an important treatment option in hyperinflammatory COVID-19. In this review, we described the key information in both case reports and clinical studies on the potential beneficial features of IL-1 inhibitors in COVID-19 patients.
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http://dx.doi.org/10.1016/j.cyto.2021.155544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052468PMC
July 2021

Biomimetic N-Doped Graphene Membrane for Proton Exchange Membranes.

Nano Lett 2021 05 13;21(10):4314-4319. Epub 2021 Apr 13.

Institute of Microelectronics, School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China.

Proton exchange membranes (PEMs) with both high selectivity and high permeance are of great demand in hydrogen-based applications, especially in fuel cells. Although graphene membranes have shown high selectivity of protons over other ions and molecules, the relatively low permeance of protons through perfect pristine graphene restricts its practical applications. Inspired by the nitrogen-assisted proton transport in biological systems, we introduced N-doping to increase the proton permeance and proposed a type of N-doped graphene membranes (NGMs) for proton exchange, which have both high proton permeance and high selectivity. Compared to the state-of-the-art commercial PEMs, the NGMs show significant increases in both areal proton conductivity (2-3 orders of magnitude) and selectivity of proton to methanol (1-2 orders of magnitude). The work realized the controllable tuning of proton permeance of the graphene membrane with N-doping and developed a new type of graphene-based PEMs with high performance for practical applications.
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http://dx.doi.org/10.1021/acs.nanolett.1c00813DOI Listing
May 2021

Delayed Identification of Adolescent Adrenal Cortical Carcinoma Initially Diagnosed as Polycystic Ovary Syndrome.

J Pediatr Adolesc Gynecol 2021 Mar 26. Epub 2021 Mar 26.

Department of Thoracic Surgery, Capital Institute of Pediatrics, Beijing, China.

Background: Adrenocortical carcinoma is a rare, isolated malignancy.

Case: A 13-year-old girl presented with secondary amenorrhea, hirsutism, and hypertension. Her clinical manifestations were interpreted as polycystic ovary syndrome and hyperinsulinemia. The rapid progression of this pubertal girl's virilization should have, but did not draw clinical attention to her malignancy. Because her condition worsened, she ultimately was transferred to our hospital because of an abdominal mass. She was diagnosed with a very large adrenal cortical carcinoma with pulmonary metastasis. The child was in the advanced stage and presented adrenal crisis after chemotherapy and mitotane treatment.

Summary And Conclusion: Rapidly progressive masculinization, with a marked increase in adrenal-derived androgens, might indicate rare adrenal neoplasms.
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http://dx.doi.org/10.1016/j.jpag.2021.03.004DOI Listing
March 2021

Regulating light olefins or aromatics production in ex-situ catalytic pyrolysis of biomass by engineering the structure of tin modified ZSM-5 catalyst.

Bioresour Technol 2021 Jun 13;330:124975. Epub 2021 Mar 13.

Department of Chemical Engineering, Norwegian University of Science and Technology, Trondheim 7491, Norway.

Valorization of biomass to olefin or aromatics harbours tremendous practical value due to growing concerns about sustainable production of chemicals. Herein, the olefin or aromatics yields of ex-situ catalytic pyrolysis of pine can be regulated by impregnating Sn on hollow-structured ZSM-5 (M-ZSM-5) and microporous ZSM-5 catalysts in fixed-bed reactor, respectively. Results showed that Sn/ZSM-5 catalyst simultaneously increased medium acidic sites and maintained strong acidic sites, which obtained the maximum carbon yield of aromatics (33.77%) due to enhanced cracking and deoxygenation reactions. In addition, Sn boosted alkylation between olefin and aromatics, generating more alkylbenzene. In contrast, Sn/M-ZSM-5 catalyst produced the highest olefins carbon yield (12.39%) because the reduction of strong acidic sites and microporous volume inhibited the olefin aromatization. Moreover, olefins were easier to desorb from Sn/M-ZSM-5 due to the enhanced mass transfer ability, which weakened the alkylation reactions. The synergistic effect harbours great significance to manipulate the distribution of products.
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http://dx.doi.org/10.1016/j.biortech.2021.124975DOI Listing
June 2021

Role of IL-6 inhibitor in treatment of COVID-19-related cytokine release syndrome.

Int J Med Sci 2021 21;18(6):1356-1362. Epub 2021 Jan 21.

Department of Clinical Laboratory Medicine, the Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, China.

Cytokine release syndrome (CRS) may be the key factor in the pathology of severe coronavirus disease 2019 (COVID-19). As a major driver in triggering CRS in patients with COVID-19, interleukin-6 (IL-6) appears to be a promising target for therapeutics. The results of inhibiting both trans- and classical- signaling with marketed IL-6 inhibitors (tocilizumab, siltuximab and sarilumab) in severe COVID-19 patients are effective based on several small studies and case reports thus far. In this review, we described the evidence of the IL-6 response in patients with COVID-19, clarified the pathogenesis of the role of IL-6-mediated CRS in severe COVID-19, and highlighted the rationale for the use of anti-IL-6 agents and key information regarding the potential features of these IL-6 inhibitors in COVID-19 patients.
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http://dx.doi.org/10.7150/ijms.53564DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893562PMC
April 2021

Decimeter-Scale Atomically Thin Graphene Membranes for Gas-Liquid Separation.

ACS Appl Mater Interfaces 2021 Mar 18;13(8):10328-10335. Epub 2021 Feb 18.

Institute of microelectronics, School of Electronics Engineering and Computer Science, Peking University, Beijing 100871, China.

Graphene holds great potential for fabricating ultrathin selective membranes possessing high permeability without compromising selectivity and has attracted intensive interest in developing high-performance separation membranes for desalination, natural gas purification, hemodialysis, distillation, and other gas-liquid separation. However, the scalable and cost-effective synthesis of nanoporous graphene membranes, especially designing a method to produce an appropriate porous polymer substrate, remains very challenging. Here, we report a facile route to fabricate decimeter-scale (∼15 × 10 cm) nanoporous atomically thin membranes (NATMs) via the direct casting of the porous polymer substrate onto graphene, which was produced by chemical vapor deposition (CVD). After the vapor-induced phase-inversion process under proper experimental conditions (60 °C and 60% humidity), the flexible nanoporous polymer substrate was formed. The resultant skin-free polymer substrate, which had the proper pore size and a uniform spongelike structure, provided enough mechanical support without reducing the permeance of the NATMs. It was demonstrated that after creating nanopores by the O plasma treatment, the NATMs were salt-resistant and simultaneously showed 3-5 times higher gas (CO) permeance than the state-of-the-art commercial polymeric membranes. Therefore, our work provides guidance for the technological developments of graphene-based membranes and bridges the gap between the laboratory-scale "proof-of-concept" and the practical applications of NATMs in the industry.
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http://dx.doi.org/10.1021/acsami.0c23013DOI Listing
March 2021

Authentication, characterization and contamination detection of cell lines, xenografts and organoids by barcode deep NGS sequencing.

NAR Genom Bioinform 2020 Sep 18;2(3):lqaa060. Epub 2020 Aug 18.

Crown Bioscience, Inc., 218 Xinghu Road, Suzhou, Jiangsu 215400, China.

Misidentification and contamination of biobank samples (e.g. cell lines) have plagued biomedical research. Short tandem repeat (STR) and single-nucleotide polymorphism assays are widely used to authenticate biosamples and detect contamination, but with insufficient sensitivity at 5-10% and 3-5%, respectively. Here, we describe a deep NGS-based method with significantly higher sensitivity (≤1%). It can be used to authenticate human and mouse cell lines, xenografts and organoids. It can also reliably identify and quantify contamination of human cell line samples, contaminated with only small amount of other cell samples; detect and quantify species-specific components in human-mouse mixed samples (e.g. xenografts) with 0.1% sensitivity; detect mycoplasma contamination; and infer population structure and gender of human samples. By adopting DNA barcoding technology, we are able to profile 100-200 samples in a single run at per-sample cost comparable to conventional STR assays, providing a truly high-throughput and low-cost assay for building and maintaining high-quality biobanks.
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http://dx.doi.org/10.1093/nargab/lqaa060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671372PMC
September 2020

Clinical types of checkpoint inhibitor-related pneumonitis in lung cancer patients: a multicenter experience.

Transl Lung Cancer Res 2021 Jan;10(1):415-429

State Key Laboratory of Respiratory Disease, National Clinical Research Centre for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Background: Checkpoint inhibitor-related pneumonitis (CIP) is not well classified according to clinical factors. We propose different clinical sub-types of CIP based on clinical factors and investigated the corresponding clinical features, treatments, and outcomes.

Methods: We conducted a multicenter retrospective study of patients with lung cancer (including non-small cell lung cancer and small cell lung cancer) who developed CIP. The clinical characteristics, radiologic features, treatments, and outcomes of CIP were analyzed.

Results: A total of 55 patients developed CIP and were classified into 3 groups as follows: 21 in the pure type (PT) group, 14 in the induced type (IT) group, and 20 in the mixed type (MT) group. The incidence of severe (grade 3-5) pneumonitis was significantly higher in the IT group than in the PT and MT groups (71.4% 14.3% 50.0%, P=0.002). Antiviral therapy was significantly more frequent in the IT group than in the PT and MT groups. Antibiotic therapy was administered in 23.8%, 71.4%, and 80.0% of patients with the PT, IT, and MT, respectively. The improvement time in the PT group was longer than that in the IT and MT groups (0.9 0.5 0.3 months, P=0.028). Patients with the PT had a better tumor response to immune checkpoint inhibitors (ICIs) than those with the other 2 types [overall response rate (ORR), 78% 31% 44%, P=0.027].

Conclusions: The clinical classification of CIP may favor strategies for treatments and predict the tumor response to ICIs.
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http://dx.doi.org/10.21037/tlcr-20-1258DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867788PMC
January 2021

CircRNA circ_0004370 promotes cell proliferation, migration, and invasion and inhibits cell apoptosis of esophageal cancer via miR-1301-3p/COL1A1 axis.

Open Med (Wars) 2021 4;16(1):104-116. Epub 2021 Jan 4.

Department of Thoracic Surgery, The First Affiliated Hospital of Kunming Medical University, No 295 Xichang Road, Kunming 650032, Yunnan, China.

Background: The aim of this study was to investigate the circ_0004370 expression in EC, its effects on cell proliferation, apoptosis, migration, invasion, and epithelial-mesenchymal transition (EMT) process, and the underlying regulatory mechanisms in EC.

Methods: The protein levels of COL1A1 and EMT-related proteins were detected by western blot. The role of circ_0004370 on cell viability, proliferation, and apoptosis was analyzed by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, and flow cytometry, respectively. The transwell assay was used to examine cell migration and invasion. The binding sites between miR-1301-3p and circ_0004370 or COL1A1 were predicted by starbase software and confirmed by dual-luciferase reporter assay and RNA pull-down assay.

Results: We discovered that circ_0004370 was remarkably upregulated in EC tissues and cells. Knockdown of circ_0004370 inhibited cell proliferation, migration as well as invasion, and promoted apoptosis , while its effect was rescued by miR-1301-3p inhibition. And circ_0004370 mediated the EMT process in EC cells. Moreover, we explored its regulatory mechanism and found that circ_0004370 directly bound to miR-1301-3p and COL1A1 was verified as a target of miR-1301-3p. COL1A1 was highly expressed in EC cells and upregulation of COL1A1 reversed the effects of miR-1301-3p on cell proliferation, migration, invasion, and apoptosis. In addition, silencing of circ_0004370 reduced tumor volumes and weights . We showed that circ_0004370/miR-1301-3p/COL1A1 axis played the critical role in EC to regulate the cell activities.

Conclusion: Circ_0004370 promotes EC proliferation, migration and invasion, and EMT process and suppresses apoptosis by regulating the miR-1301-3p/COL1A1 axis, indicating that circ_0004370 may be used as a potential therapeutic target for EC.
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http://dx.doi.org/10.1515/med-2021-0001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801883PMC
January 2021

Synthesis of [email protected] [email protected] Nano-Octahedra and Their Improved MOR Activity.

Angew Chem Int Ed Engl 2021 Mar 25;60(14):7675-7680. Epub 2021 Feb 25.

Department of Chemistry, State University of New York at Binghamton, Binghamton, NY, 13902, USA.

Fabrication of 3d metal-based [email protected] nanocatalysts with engineered Pt-surfaces provides an effective approach for improving the catalytic performance. The challenges in such preparation include shape control of the 3d metallic cores and thickness control of the Pt-based shells. Herein, we report a colloidal seed-mediated method to prepare octahedral [email protected] [email protected] nanocrystals using CuNi octahedral cores as the template. By precisely controlling the synthesis conditions including the deposition rate and diffusion rate of the shell-formation through tuning the capping ligand, reaction temperature, and heating rate, uniform Pt-based shells can be achieved with a thickness of <1 nm. The resultant carbon-supported [email protected] [email protected] nano-octahedra showed superior activity in electrochemical methanol oxidation reaction (MOR) compared with the commercial Pt/C catalysts and carbon-supported [email protected] nano-polyhedron counterparts.
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http://dx.doi.org/10.1002/anie.202014144DOI Listing
March 2021

Multiview Feature Learning With Multiatlas-Based Functional Connectivity Networks for MCI Diagnosis.

IEEE Trans Cybern 2020 Dec 11;PP. Epub 2020 Dec 11.

Functional connectivity (FC) networks built from resting-state functional magnetic resonance imaging (rs-fMRI) has shown promising results for the diagnosis of Alzheimer's disease and its prodromal stage, that is, mild cognitive impairment (MCI). FC is usually estimated as a temporal correlation of regional mean rs-fMRI signals between any pair of brain regions, and these regions are traditionally parcellated with a particular brain atlas. Most existing studies have adopted a predefined brain atlas for all subjects. However, the constructed FC networks inevitably ignore the potentially important subject-specific information, particularly, the subject-specific brain parcellation. Similar to the drawback of the ``single view'' (versus the ``multiview'' learning) in medical image-based classification, FC networks constructed based on a single atlas may not be sufficient to reveal the underlying complicated differences between normal controls and disease-affected patients due to the potential bias from that particular atlas. In this study, we propose a multiview feature learning method with multiatlas-based FC networks to improve MCI diagnosis. Specifically, a three-step transformation is implemented to generate multiple individually specified atlases from the standard automated anatomical labeling template, from which a set of atlas exemplars is selected. Multiple FC networks are constructed based on these preselected atlas exemplars, providing multiple views of the FC network-based feature representations for each subject. We then devise a multitask learning algorithm for joint feature selection from the constructed multiple FC networks. The selected features are jointly fed into a support vector machine classifier for multiatlas-based MCI diagnosis. Extensive experimental comparisons are carried out between the proposed method and other competing approaches, including the traditional single-atlas-based method. The results indicate that our method significantly improves the MCI classification, demonstrating its promise in the brain connectome-based individualized diagnosis of brain diseases.
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http://dx.doi.org/10.1109/TCYB.2020.3016953DOI Listing
December 2020

Analysis of Human Acupoint Biological Information and Neural Electric Activity Based on Ultrasonographic Image.

World Neurosurg 2021 05 19;149:481-491. Epub 2020 Nov 19.

Department of Rehabilitation Medicine, The First Affiliated Hospital of Gannan Medical University, Zhanggong District, Ganzhou, Jiangxi, China. Electronic address:

To find out the bioelectric signal discriminative features of different acupoints at the same time and at the same acupoint, and then analyze these differences to determine the type of acupuncture points and assist in judging the patient's condition, a hardware system was designed that can collect subcutaneous potential information of acupoints. Then, the changes of ultrasonic images were analyzed after acupuncture at Zu San Li, as well as the influence of acupuncture at Zu San Li on vagal nerve activity. The hardware system is composed of 4 parts: an analog circuit module, a digital circuit module, a power module, and a host personal computer. The power module adopted dual-module power supply mode, which was composed of MC78M05CT and LM2576 and their related peripheral circuits to ensure the stability of the circuit. The analog circuit module collected the electric signals from the 2 acupoints of Zu San Li and Shang Ju Xu of the volunteers' calves through a multistage electrode probe. In the amplifier module, Butterworth filter and 50 HZ notch are used to reduce the noise of electric signals. The filtered and amplified electric signals are converted into A/D converter in the digital circuit module. Serial communication is used to transmit data to the upper computer of the personal computer to compare the noise reduction effect of acupoint information, multiple sequences of different acupoints, and multiple sequences of the same acupoint. The influence of acupuncture at the Zu San Li acupoint on the muscle tension around the acupoint was investigated by shear wave acoustic elastic imaging technology. The influence of acupuncture at this acupoint was explored on vagal nerve activity. The results show that the wavelet noise reduction method can greatly filter out the interference signals. In the case of artificial stimulation, the signal waveform of each twist shows a big increase, and the comparison shows that there is no significant change in the frequency of other bands. Through ultrasonic image evaluation, it was found that acupuncture at Zu San Li can significantly affect the mean value of Young modulus. After acupuncture at Zu San Li, the vagal activity was increased and the balance ratio of sympathetic vagal activity was reduced. Part of the potential frequency of the acupoint is extremely active, which is the response potential of the subcutaneous induction tissue at the acupoint, which proved the feasibility of collecting electric signals around the acupoints in this kind of hardware system. It is of great significance to diagnose the disease according to the characteristics of acupoint electric signals.
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http://dx.doi.org/10.1016/j.wneu.2020.11.062DOI Listing
May 2021

Multi-Vehicle Cooperative Target Tracking with Time-Varying Localization Uncertainty via Recursive Variational Bayesian Inference.

Sensors (Basel) 2020 Nov 13;20(22). Epub 2020 Nov 13.

School of Automotive and Traffic Engineering, Jiangsu University, Zhenjiang 212013, China.

Cooperative target tracking by multiple vehicles connected through inter-vehicle communication is a promising way to improve the estimation of target state. The effectiveness of cooperative tracking closely depends on the accuracy of relative localization between host and cooperative vehicles. However, the localization signal usually provided by the satellite-based navigation system is rather susceptible to dynamic driving environment, thus influencing the effectiveness of cooperative tracking. In order to implement reliable cooperative tracking, especially when the statistical characteristic of the relative localization noise is time-varying and uncertain, this paper presents a recursive Bayesian framework which jointly estimates the state of the target and the cooperative vehicle as well as the localization noise parameter. An online variational Bayesian inference algorithm is further developed to achieve efficient recursive estimate. The simulation results verify that our proposed algorithm can effectively boost the accuracy of target tracking when the localization noise dynamically changes over time.
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http://dx.doi.org/10.3390/s20226487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697773PMC
November 2020

Learning Robust Discriminant Subspace Based on Joint L2,p- and L2,s-Norm Distance Metrics.

IEEE Trans Neural Netw Learn Syst 2020 Nov 12;PP. Epub 2020 Nov 12.

Recently, there are many works on discriminant analysis, which promote the robustness of models against outliers by using L₁- or L2,1-norm as the distance metric. However, both of their robustness and discriminant power are limited. In this article, we present a new robust discriminant subspace (RDS) learning method for feature extraction, with an objective function formulated in a different form. To guarantee the subspace to be robust and discriminative, we measure the within-class distances based on L2,s-norm and use L2,p-norm to measure the between-class distances. This also makes our method include rotational invariance. Since the proposed model involves both L2,p-norm maximization and L2,s-norm minimization, it is very challenging to solve. To address this problem, we present an efficient nongreedy iterative algorithm. Besides, motivated by trace ratio criterion, a mechanism of automatically balancing the contributions of different terms in our objective is found. RDS is very flexible, as it can be extended to other existing feature extraction techniques. An in-depth theoretical analysis of the algorithm's convergence is presented in this article. Experiments are conducted on several typical databases for image classification, and the promising results indicate the effectiveness of RDS.
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http://dx.doi.org/10.1109/TNNLS.2020.3027588DOI Listing
November 2020

Catalytic Deoxygenation of Xylitol to Renewable Chemicals: Advances on Catalyst Design and Mechanistic Studies.

Chem Rec 2021 Jan 12;21(1):133-148. Epub 2020 Nov 12.

State Key Laboratory of Heavy Oil Processing, College of, Chemical Engineering, China University of Petroleum, No. 66 Changjiang West Road, Qingdao, Shandong Province, 266580, China.

Xylitol is commonly known as one of the top platform intermediates for biomass conversion. Catalytic deoxygenation of xylitol provides an atomic and energetic efficient way to produce a variety of renewable chemicals including ethylene glycol, 1,2-propanediol, lactic acid and 1,4-anhydroxylitol. Despite a few initial attempts in converting xylitol into those products, improving catalyst selectivity towards C-O and C-C cleavage reactions remains a grand challenge in this area. To our best knowledge, there is lack of comprehensive review to summarize the most recent advances on catalyst design and mechanisms in deoxygenation of xylitol, offering important perspective into future development of xylitol transformation technologies. Therefore, in this mini-review, we have critically discussed the conversion routes involved in xylitol deoxygenation over solid catalyst materials, the nanostructures of supported metal catalysts for C-H, C-C and C-O bond cleavage reactions, and mechanistic investigation for xylitol conversion. The outcome of this work provides new insights into rational design of effective deoxygenation catalyst materials for upgrading of xylitol and future process development in converting hemicellulosic biomass.
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http://dx.doi.org/10.1002/tcr.202000101DOI Listing
January 2021

[Clinical and genetic analysis of a child with neonatal severe parathyroidism].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2020 Nov;37(11):1247-1249

Department of Endocrinology, Children's Hospital Affiliated to Capital Institute of Pediatrics, Beijing 100020, China.

Objective: To explore the genetic basis for a child with neonatal severe hyperparathyroidism.

Methods: Genomic DNA was extracted from peripheral blood samples from the patient and her parents. Whole exome sequencing was carried out to screen potential mutations. Suspected mutation was verified by Sanger sequencing.

Results: The proband was found to carry compound heterozygous variants c.179G>A (p.Cys60Tyr) and c.1525G>A (p.Gly509Arg) of the CaSR gene. The c.179G>A variant was derived from her mother and was unreported previously. The c.1525G>A variant was derived from her father and known to be pathogenic.

Conclusion: The compound heterozygous variants of c.179G>A and c.1525G>A of the CaSR gene probably underlie the disease in the patient. The results of genetic testing has enabled diagnosis and genetic counseling for her family.
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http://dx.doi.org/10.3760/cma.j.cn511374-20191118-00587DOI Listing
November 2020

Reversing Titanium Oligomer Formation towards High-Efficiency and Green Synthesis of Titanium-Containing Molecular Sieves.

Angew Chem Int Ed Engl 2021 Feb 21;60(7):3443-3448. Epub 2020 Dec 21.

State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao, 266580, China.

Green and efficient synthesis of titanium-containing molecular sieves is limited by the quantity of environmentally unfriendly additives and complicated synthesis procedures required. Oligomerization of Ti monomers into anatase TiO is the typical outcome of such procedures because of a mismatch between hydrolysis rates of Si and Ti precursors. We report a simple and generic additive-free route for the synthesis of Ti-containing molecular sieves (MFI, MEL, and BEA). This approach successfully reverses the formation of Ti oligomers to match hydrolysis rates of Ti and Si species with the assistance of hydroxyl free radicals generated in situ from ultraviolet irradiation. Moreover, fantastic catalytic performance for propene epoxidation with H and O was observed. Compared with the conventional hydrothermal method, this approach opens up new opportunities for high-efficiency, environmentally benign, and facile production of pure titanium-containing molecular sieves.
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http://dx.doi.org/10.1002/anie.202011821DOI Listing
February 2021

Protective Effect of Ursolic Acid in Prunella vulgaris L on LPS-induced asthenozoospermia via Bcl-2/Bax Apoptosis Signaling Pathway.

Curr Pharm Biotechnol 2020 Oct 27. Epub 2020 Oct 27.

Department of Urology and Andrology, Yunnan Provincial Hospital of Traditional Chinese Medicine, 120 Guanghua Street, Kunming, Yunnan 650000. China.

Background: Asthenozoospermia, also known as lack of sperm motility, accounts for about 27.8% of male infertility as a separate factor, and is often associated with abnormal quantity and morphology of spermatozoa. Therefore, oligozoospermia has become one of the most important factors affecting male infertility.

Methods: Ursolic acid (UA), also known as wusu acid, is the main active component isolated from Prunella vulgaris L and has a variety of pharmacological effects. However, the protective effect of UA on asthenozoospermia disease has not been reported. In the current study, the purpose of this study was to investigate the regulatory effect of UA in rats with LPSinduced asthenozoospermia disease. SD rats were treated with 5 mg/kg LPS respectively.

Results: After different concentrations of UA were infused into the stomach of SD rats, microscopy, flow cytometry, enzyme-linked immunosorbent assay (ELISA), qRT-PCR and western blot were used to detect sperm motility, apoptosis, the levels of TNF-α, IL-1β and IL-6, and Bcl-2/Bax apoptosis pathway related proteins in rat serum and epididymis tissues.

Discussion: Compared with normal group, the sperm motility and Bcl-2 level in LPS group decreased significantly, while the expression of inflammatory factors and Bax proteins increased significantly (P<0.05). Compared with LPS group, UA intervention group has the opposite result and dose dependence.

Conclusion: This study shows that UA can protect LPS-induced asthenozoospermia of rat by increasing sperm density and motility, regulating Bcl-2/Bax apoptosis pathway and reducing inflammatory apoptosis response. This experiment provides ideas for improving the clinical treatment of infertile patients with oligoasthenospermia.
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http://dx.doi.org/10.2174/1389201021666201027155413DOI Listing
October 2020
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