Publications by authors named "Xiaobing Xu"

43 Publications

Antitumor CD8 T cell responses in glioma patients are effectively suppressed by T follicular regulatory cells.

Exp Cell Res 2021 Sep 9;407(2):112808. Epub 2021 Sep 9.

Department of Neurosurgery, Shunde Hospital, Southern Medical University (The First People's Hospital of Sunde), Foshan, Guangdong, China. Electronic address:

Regulatory T (Treg) cells are thought to contribute to tumor pathogenesis by suppressing tumor immunosurveillance and antitumor immunity. T follicular regulatory (Tfr) cells are a recently characterized Treg subset that expresses both the Treg transcription factor (TF) Foxp3 and the T follicular helper (Tfh) TF Bcl-6. The role of Tfr cells in glioma patients remains unclear. In this study, we found that the level of Tfr cells, identified as Foxp3Bcl-6 CD4 T cells, was significantly elevated in tumor-infiltrating CD4 T cells from resected glioma tumors. Both Tfr cells and Treg cells significantly suppressed the proliferation and the cytotoxic capacity of CD8 T cells toward glioma tumor cells, and the suppression was positively associated with the proportion of Tfr cells and Treg cells, respectively. Tfr and Treg cells from glioma tumor samples demonstrated higher suppression potency than those from healthy blood samples and glioma blood samples. Interestingly, canonical CXCR5 Treg cells could suppress both CXCR5 and CXCR5 CD8 T cells, albeit with stronger potency toward CXCR5 CD8 T cells. However, Tfr cells presented much higher suppression potency toward CXCR5 CD8 T cells, whereas CXCR5 CD8 T cells are a potent CD8 T cell subset previously described to have antiviral and antitumor roles. Overall, these data indicate that Tfr cells are enriched in glioma tumors and have suppressive capacity toward CD8 T cell-mediated effector functions.
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http://dx.doi.org/10.1016/j.yexcr.2021.112808DOI Listing
September 2021

Associations among Audiometric, Doppler Hydroacoustic, and Subjective Outcomes of Venous Pulsatile Tinnitus.

ORL J Otorhinolaryngol Relat Spec 2021 Jul 26:1-10. Epub 2021 Jul 26.

Department of Otology and Skull Base Surgery, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai, China.

Objective: Venous pulsatile tinnitus (PT) has received increasing attention recently. As analyses of psychophysical and neuropsychological dimensions of venous PT are lacking, this study aimed to quantitatively and qualitatively investigate the correlation among audiometric, hydroacoustic, and subjective outcomes in patients with PT.

Methods: Fifty-five venous PT patients, with or without sigmoid sinus wall anomalies (SSWAs), were subdivided into SSWAs (n = 30) and non-SSWAs (n = 25) groups. Audiometric and hemodynamic evaluations were assessed. Questionnaires including the Tinnitus Handicap Inventory, Hospital Anxiety and Depression Scale (HADS), and Athens Insomnia Scale (AIS) were deployed to evaluate the psychological impacts of PT.

Results: Among 55 subjects, PT frequency-related pure-tone audiometry (PTA) was significantly different between ipsilesional non-PT frequency-related PTA (p < 0.01), ipsilateral jugular vein compression PTA (p < 0.01), and contralesional ear PTA (p < 0.01). In contrast with the pulsatility index and flow velocity, bilateral EOET and flow volume were significantly different (p < 0.01). Of the 3 questionnaire types, there was a strong correlation between HADS anxiety and AIS scores (r = 0.658, p < 0.01). The duration of PT was not correlated with subjective outcomes, and there was no statistical significance found among audiometric, hemodynamic, and subjective outcomes between SSWAs and non-SSWAs groups.

Conclusions: (1) The duration of PT was irrelevant to the increase of PTA. (2) Venous PT is the perception of vascular flow sound, in which hydroacoustic characteristics can be highly independent. (3) Anxiety, depression, and sleep disorders commonly prevail among PT patients.
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http://dx.doi.org/10.1159/000517610DOI Listing
July 2021

Circular RNA circ_0001588 sponges miR-211-5p to facilitate the progression of glioblastoma via up-regulating YY1 expression.

J Gene Med 2021 Jun 9:e3371. Epub 2021 Jun 9.

Radiation Oncology Department of Gastrointestinal & Urinary & Musculoskeletal, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Insititute, Shenyang, Liaoning Province, 110042, PR China.

Background: As the most common and detrimental brain tumor with high invasiveness and poor prognosis, glioblastoma (GBM) has severely threatened people's health globally. Therefore, it is of great importance and necessary to identify the molecular mechanisms involved in tumorigenesis and development, thus contributing to potential therapeutic targets and strategies.

Methods: The level of circ_0001588 was detected in 68 pairs of GBM tissues and adjacent normal tissues and human glioma cell lines via a real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Then, the effect of circ_0001588 on the proliferation, migration and invasion of glioma cells was evaluated. In addition, potential downstream targets of circ_0001588 were forecasted by circBANK and Starbase. Their interaction was confirmed by introducing luciferase reporter assays. Moreover, sh-circ_0001588 transfected U251 cells were used to form tumors in vivo. Finally, the functional mechanism of circ_0001588 was identified by qRT-PCR, western blotting, xenograft and immunohistochemistry (IHC) assays.

Results: The expression of circ_0001588 is markedly up-regulated in GBM tissues and human gliomas cells. Additionally, increased expression of circ_0001588 is positively relevant with poor survival in GBM patients. The down-regulation of circ_0001588 distinctly inhibits the proliferation, migration and invasion of GBM in vitro, as well as tumor growth in vivo. Moreover, knockdown of circ_0001588 reduces the tumor volume and weight, enhances the relative IHC staining index of E-cadherin and decreases the relative IHC staining index of Ki-67, Yin Yang 1 (YY1) and vinmentin in vivo. Mechanistically, circ_0001588 locates in the cytoplasm, which is directly bound with miR-211-5p. Furthermore, circ_0001588 can positively regulate YY1 via sponging miR-211-5p. Moreover, circ_0001588 accelerates the proliferation, migration and invasion of GBM by modulating miR-211-5p/YY1 signaling.

Conclusions: These results illustrate a new circ_0001588/miR-211-5p/YY1 regulatory signaling axis in GBM.
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http://dx.doi.org/10.1002/jgm.3371DOI Listing
June 2021

Nanostructured [email protected] graphene oxide as an efficient hydrogen evolution electrocatalyst in alkaline electrolyte.

J Colloid Interface Sci 2021 Nov 27;601:570-580. Epub 2021 May 27.

Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for Nano Technology, Nanjing University, Nanjing 210093, China.

Metal-organic frameworks (MOFs), serving as precursors or templated to construct nanomaterials, which have gained great attentions in the field of electrocatalysis. However, their applications still remain some challenges due to poor conductivity and easy agglomeration. In this work, the MOFs-derived [email protected] deposited on reduced Graphene Oxide (rGO) surface is designed by using a facile hydrothermal procedure. Attribute to the enlarged active surface area of the nanostructure and the strong synergistic effect between NiCoS and NiCoO, as well as the excellent conductivity of rGO. The [email protected] catalyst displays ultrahigh hydrogen evolution reaction (HER) property and excellent stability, only need an overpotential of η = 95 mV to attain 10 mA cm and deliver a small Tafel slope of b = 52 mV dec in 1 M KOH. This work can provide a window to construct and develop new noble metal-free HER catalysts base on Ni-MOFs served as precursors.
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http://dx.doi.org/10.1016/j.jcis.2021.05.148DOI Listing
November 2021

A moisture-enabled fully printable power source inspired by electric eels.

Proc Natl Acad Sci U S A 2021 Apr;118(16)

Beijing Graphene Institute, 100095 Beijing, China

Great efforts have been made to build integrated devices to enable future wearable electronics; however, safe, disposable, and cost-effective power sources still remain a challenge. In this paper, an all-solid-state power source was developed by using graphene materials and can be printed directly on an insulating substrate such as paper. The design of the power source was inspired by electric eels to produce programmable voltage and current by converting the chemical potential energy of the ion gradient to electric energy in the presence of moisture. An ultrahigh voltage of 192 V with 175 cells in series printed on a strip of paper was realized under ambient conditions. For the planar cell, the mathematical fractal design concept was adapted as printed patterns, improving the output power density to 2.5 mW cm, comparable to that of lithium thin-film batteries. A foldable three-dimensional (3D) cell was also achieved by employing an origami strategy, demonstrating a versatile design to provide green electric energy. Unlike typical batteries, this power source printed on flexible paper substrate does not require liquid electrolytes, hazardous components, or complicated fabrication processes and is highly customizable to meet the demands of wearable electronics and Internet of Things applications.
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http://dx.doi.org/10.1073/pnas.2023164118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072409PMC
April 2021

Good News or Bad News? How Message Framing Influences Consumers' Willingness to Buy Green Products.

Front Psychol 2020 19;11:568586. Epub 2021 Jan 19.

School of Management, Hainan University, Haikou, China.

Despite the growing social interest in green products, companies often find it difficult to find effective strategies to induce consumers to purchase green products or engage in other environmentally friendly behaviors. To address this situation, we examined the favorable or unfavorable effects of positive and negative message frames on consumers' willingness to consume green products in different psychological distance contexts. Through two Studies, we found that the positive information framework played a more pronounced role in context when consumers were in closer spatial distances. More importantly, we found that the emotional factors of fear and hope were intrinsic causes of this phenomenon. Correspondingly, the negative information framework played a better facilitating role in context with farther spatial distance, while shame and pride were the emotions responsible for this effect. Finally, we discuss the theoretical and managerial implications of our work, as well as its limitations and future research directions.
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http://dx.doi.org/10.3389/fpsyg.2020.568586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874151PMC
January 2021

Hydroacoustic analysis and extraluminal compression surgical insights of venous pulsatile tinnitus.

Auris Nasus Larynx 2021 Oct 17;48(5):852-863. Epub 2021 Jan 17.

Fudan University, Shanghai, China; Department of Otology and Skull Base Surgery, Eye Ear Nose & Throat Hospital, Fudan University, Shanghai 200031, China; Shanghai Auditory Medical Center, Shanghai, China; Key laboratory of Hearing Science, Ministry of Health, Shanghai, China. Electronic address:

Objective: This study aimed to quantitatively and qualitatively evaluate the hydroacoustic changes from "presence" to "disappearance" of pulsatile tinnitus (PT) with the extraluminal compression surgical technique. The recent issues of concern pertaining to the hydroacoustic characteristics of sigmoid sinus wall anomalies and distal transverse sinus stenosis (dTSS) were discussed.

Methods: This study was based on a retrospective case series. Seventy-seven patients with PT and transverse-sigmoid sinus enlargement with or without transverse-sigmoid sinus junction anomalies and transverse sinus stenosis (TSS) who had undergone extraluminal compression surgery under local anesthesia were included. Management of intractable intraoperative challenges and techniques for reversal extraluminal compression were introduced. Anatomical measurements, intraoperative color-coded Doppler ultrasonography, spectro-temporal analysis, and computational fluid dynamics were employed to analyze the hydroacoustic characteristics of PT.

Results: The efficacy of the extraluminal compression technique was evident with the significant reduction in peak turbulent kinetic energy, vorticity, and mean pressure gradient at the transverse-sigmoid junction, resulting in over 20% reduction in PT amplitude. dTSS is a common finding in patients with PT exhibiting transverse-sigmoid sinus enlargement. Patients with dTSS presented with significant differences in hemodynamic characteristics as compared to those without. Linear regression analysis showed that the flow disturbance (turbulent kinetic energy and vorticity) was closely associated with the degree of dTSS, whereas the flow amplitude was not related to the degree or location of TSS. Low-pulsatory vortex flow at the transverse-sigmoid junction was visualized during an intraoperative color-coded Doppler examination, and the displayed low-frequency PT sound corresponded to the patients' subjective perception of PT.

Conclusion: (1) A reduction of over 20% of the flow-induced noise is the therapeutic goal of extraluminal compression technique. Since reductions in the magnitude of hemodynamic parameters, including turbulent kinetic energy, vorticity, and mean pressure gradient, render the flow-induced noise inaudible, besides sigmoid sinus wall anomalies, it is likely that PT develops from the aggregation of flow-based pathologies. (2) Although dTSS and diverticulum may greatly affect the hemodynamics at the transverse-sigmoid junction, in contrast to dehiscence, dTSS and diverticulum may not be the limiting factors for PT development.
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http://dx.doi.org/10.1016/j.anl.2021.01.013DOI Listing
October 2021

Differential diagnosis for esophageal protruded lesions using a deep convolution neural network in endoscopic images.

Gastrointest Endosc 2021 06 13;93(6):1261-1272.e2. Epub 2020 Oct 13.

Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background And Aims: Recent advances in deep convolutional neural networks (CNNs) have led to remarkable results in digestive endoscopy. In this study, we aimed to develop CNN-based models for the differential diagnosis of benign esophageal protruded lesions using endoscopic images acquired during real clinical settings.

Methods: We retrospectively reviewed the images from 1217 patients who underwent white-light endoscopy (WLE) and EUS between January 2015 and April 2020. Three deep CNN models were developed to accomplish the following tasks: (1) identification of esophageal benign lesions from healthy controls using WLE images; (2) differentiation of 3 subtypes of esophageal protruded lesions (including esophageal leiomyoma [EL], esophageal cyst (EC], and esophageal papilloma [EP]) using WLE images; and (3) discrimination between EL and EC using EUS images. Six endoscopists blinded to the patients' clinical status were enrolled to interpret all images independently. Their diagnostic performances were evaluated and compared with the CNN models using the area under the receiver operating characteristic curve (AUC).

Results: For task 1, the CNN model achieved an AUC of 0.751 (95% confidence interval [CI], 0.652-0.850) in identifying benign esophageal lesions. For task 2, the proposed model using WLE images for differentiation of esophageal protruded lesions achieved an AUC of 0.907 (95% CI, 0.835-0.979), 0.897 (95% CI, 0.841-0.953), and 0.868 (95% CI, 0.769-0.968) for EP, EL, and EC, respectively. The CNN model achieved equivalent or higher identification accuracy for EL and EC compared with skilled endoscopists. In the task of discriminating EL from EC (task 3), the proposed CNN model had AUC values of 0.739 (EL, 95% CI, 0.600-0.878) and 0.724 (EC, 95% CI, 0.567-0.881), which outperformed seniors and novices. Attempts to combine the CNN and endoscopist predictions led to significantly improved diagnostic accuracy compared with endoscopists interpretations alone.

Conclusions: Our team established CNN-based methodologies to recognize benign esophageal protruded lesions using routinely obtained WLE and EUS images. Preliminary results combining the results from the models and the endoscopists underscored the potential of ensemble models for improved differentiation of lesions in real endoscopic settings.
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http://dx.doi.org/10.1016/j.gie.2020.10.005DOI Listing
June 2021

LncRNA MACC1-AS1/MACC1 enhances the progression of glioma via regulating metabolic plasticity.

Cell Cycle 2020 09 20;19(18):2286-2297. Epub 2020 Aug 20.

Department of Neurosurgery, Shunde Hospital of Southern Medical University , Foshan, China.

This study plans to investigate the effects of long-noncoding RNA MACC1-AS1 on glioma cells and its mechanism at metabolic plasticity angle. The MACC1-AS1 level was identified both in glioma tissues and in cells. Then the effects of MACC1-AS1 abnormal level on cell viability, apoptosis, the expression of apoptosis associated protein, glucose metabolism and redox status were measured in A172 and U251 cells by different methods. Furthermore, the interaction of MACC1-AS1 and MACC1 in glioma cells was investigated and the role of AMPK pathway was specifically examined. Our results demonstrated that MACC1-AS1 level was high in glioma tissues and cells, and MACC1-AS1 overexpression was closely associated with poor prognosis of glioma. Notably, under glucose deprivation, the MACC1-AS1 level was significantly increased, and overexpression of MACC1-AS1 increased cell viability but inhibited apoptosis. Also, MACC1-AS1 overexpression obviously increased the levels of GLUT1, HK2, G6PD, MCT1, ATP, lactate and NAPDH as well as promoted the activities of HK2 and LDHA, while reduced ROS level and the ratio of NADP+/NAPDH. In particular, the effects of proliferation, apoptosis and metabolic plasticity of glioma cells caused by MACC1-AS1 overexpression were achieved by positively regulating MACC1, and MACC1-AS1 promoted MACC1 expression via the AMPK pathway. In conclusions, the MACC1-AS1/MACC1 axis exertes the tumor-promoting effect by regulating glucose metabolism and redox homeostasis in glioma cells by activating the AMPK signals.
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http://dx.doi.org/10.1080/15384101.2020.1795595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7513851PMC
September 2020

Analysis of difficulties and influencing factors in treatment of appendiceal abscess in children with laparoscopic surgery.

Minerva Pediatr 2020 Jul 20. Epub 2020 Jul 20.

Department of Emergency Medicine, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, P.R.China -

The purpose of this study was to investigate and analyze the difficulties and influencing factors in the laparoscopic surgery for appendiceal abscess in children. 46 patients with appendiceal abscess receiving laparoscopic surgery (laparoscopic surgery group) in Xuzhou Children's Hospital from January 2012 to March 2017 were retrospectively analyzed. The surgery was performed using the 3-hole method, the 30° lens was placed in umbilical region, and the left lower abdomen and superior pubis were the operating holes. If the appendix was thick and big, 10 mm Trocar was replaced on the superior pubis and the appendix was removed, followed by pelvic or lower abdominal drainage via the superior pubis incision. The laparoscopic surgery group was compared with 45 cases of laparotomy (traditional surgery group) for appendiceal abscess performed by the same group of operators in the past, and the perioperative period and postoperative complications between the two groups were compared. The child patients' age, history of abdominal pain and pre-diagnosis treatment were compared within the laparoscopic surgery group. Compared with those in traditional surgery group, the surgery time in laparoscopic surgery group had no statistically significant difference (p>0.05), the incidence rate of complications was lower, and the difference was statistically significant (p<0.05). In laparoscopic surgery group, the surgery time of child patients aged ≥6 years old with the medical history for more than 5 days and unreasonable preoperative treatment was longer than that of child patients aged <6 years old with the medical history for less than or equal to 5 days and reasonable preoperative treatment, and the difference was statistically significant (p<0.05). Compared with the traditional surgery, laparoscopic surgery for appendiceal abscess in children can significantly reduce the postoperative complications; at the same time, the history of abdominal pain and preoperative treatment are the influencing factors of surgery.
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http://dx.doi.org/10.23736/S0026-4946.20.05687-XDOI Listing
July 2020

Pulsatile tinnitus caused by an aberrant artery running over the surface of mastoid bone.

Auris Nasus Larynx 2021 Dec 15;48(6):1181-1188. Epub 2020 Jul 15.

Department of Otology and Skull Base Surgery, Eye Ear Nose & Throat Hospital, Fudan University, Shanghai 200031, China; Shanghai Auditory Medical Center, Shanghai, China; Key laboratory of Hearing Science, Ministry of Health, Shanghai, China. Electronic address:

Objective: Pulsatile tinnitus (PT) caused by an aberrant artery is rare. We report an unprecedented cause of PT resulting from an aberrant artery coursing the mastoid surface, and qualitatively discuss the pathophysiology of PT.

Methods: This case study reports a 41-year-old woman who presented with persistent PT at her right retromastoid region. Contrast-enhanced computed tomography revealed an aberrant branch of the artery that coursed over the cortex of the mastoid bone. Surgical ligation of this aberrant artery was performed under local anesthesia.

Results: Intraoperative findings suggested that PT transmitted via bone-conduction route due to the direct contact of the vascular and mastoid surface. PT was completely resolved upon surgical removal of this causative segment. Ultrasonographic and hemodynamic analysis showed that the turbulent kinetic energy and high regional wall pressure were the major contributory factors causing PT. Spectro-temporal analysis showed that PT fluctuates at frequency 500~2000 Hz, which differs from those of venous PT.

Conclusion: Judicious preoperative and intraoperative assessments of PT ensure the surgical efficacy of PT. Objective ultrasonographic and computational studies can provide detailed hydroacoustic characteristics of PT.
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http://dx.doi.org/10.1016/j.anl.2020.06.012DOI Listing
December 2021

Lowly expressed lncRNA PVT1 suppresses proliferation and advances apoptosis of glioma cells through up-regulating microRNA-128-1-5p and inhibiting PTBP1.

Brain Res Bull 2020 10 17;163:1-13. Epub 2020 Jun 17.

Department of Neurosurgery, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, 528300, Guangdong, China. Electronic address:

Background: Glioma is a primary intracranial malignancy with poor prognosis, of which the pathogenesis remains to be elucidated. Therein, the aim of this study is to discuss the impacts of lncRNA plasmacytoma variant translocation 1 (PVT1)/microRNA-128-1-5p (miR-128-1-5p)/polypyrimidine tract-binding protein 1 (PTBP1) axis on the biological characteristics of glioma cells.

Methods: Glioma tissue samples (72 cases) and normal brain tissue samples (35 cases) were harvested. The expression of PVT1, miR-128-1-5p and PTBP1 in glioma tissues and cells was detected. Glioma cells were transfected with sh-PVT1, miR-128-1-5p mimics or miR-128-1-5p inhibitors to verify the impacts of PVT1 and miR-128-1-5p on DNA damage, cell colony formation, invasion, proliferation, migration and apoptosis of glioma U87 and U251 cells. The growth of transplanted tumor was tested by tumor xenograft in nude mice. The combination of PVT1 and miR-128-1-5p and the targeting relationship between miR-128-1-5p and PTBP1 were verified.

Results: PVT1 and PTBP1 expression was enhanced and miR-128-1-5p expression was degraded in glioma tissues and cells. Overexpressed miR-128-1-5p and lowly-expressed PVT1 promoted DNA damage, suppressed colony formation, invasion, proliferation and migration as well as boosted apoptosis of U251 and U87 cells. Up-regulating miR-128-1-5p and down-regulating PVT1 reduced transplanted tumor volume and weight of glioma in mice. Low expression miR-128-1-5p reversed the effect of low expression PVT1 on the biological characteristics of glioma cells. PVT1 specifically bound to miR-128-1-5p and PTBP1 was the target gene of miR-128-1-5p.

Conclusion: This study suggests that down-regulated PVT1 or up-regulated miR-128-1-5p boosts apoptosis and attenuates proliferation of glioma cells by inhibiting PTBP1 expression. This study is essential for finding new therapeutic targets for glioma.
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http://dx.doi.org/10.1016/j.brainresbull.2020.06.006DOI Listing
October 2020

LncRNA NNT-AS1 promote glioma cell proliferation and metastases through miR-494-3p/PRMT1 axis.

Cell Cycle 2020 07 18;19(13):1621-1631. Epub 2020 May 18.

Department of Neurosurgery, Shunde Hospital of Southern Medical University (The First People's Hospital of Shunde Foshan) , Foshan, Guangdong, China.

Long noncoding RNAs (lncRNAs) are key players in cancer progression. However, the function of lncRNA NNT-AS1 on glioma is unclear. In the present study, a total of 73 tumor tissues and matched adjacent non-tumor tissues were collected, and glioma cell lines were cultured . mRNA expression was tested using RT-qPCR. The protein expression level was determined using the western blot assay, cell proliferation was measured using the CCK-8 and BrdU proliferation assay, and the cell cycle, cell migration and invasion were determined using flow cytometry analysis, the wound healing assay and transwell, respectively. The results showed that lncNNT-AS1 is significantly up-regulated during the early stages of glioma. In particular, high levels of NNT-AS1 are observed in glioma cell lines compared to human astrocyte (HA) cells. Furthermore, the inhibition of lnc-NNT-AS1 by siRNA interfere attenuates the cell viability, proliferation, migration and invasion of glioma cell lines. Mechanistically, the inhibition of NNT-AS1 directly interacted with miRNA-494-3p, and positively regulated the downstream target PRMT1 . Further study proved that the overexpression of miRNA-494-3p and the inhibition of PRMT1 could attenuate both glioma cell proliferation and metastases. Collectively, our results indicated that the miR-494-3p-PRMT1 axis is involved the tumor-suppressive effects of NNT-AS1 inhibition, which sheds new light on lncRNA-directed diagnostics and the therapeutics of glioma.
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http://dx.doi.org/10.1080/15384101.2020.1762037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469481PMC
July 2020

WBSCR22 confers cell survival and predicts poor prognosis in glioma.

Brain Res Bull 2020 08 5;161:1-12. Epub 2020 May 5.

Department of Neurosurgery, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan 528300, Guangdong, China. Electronic address:

Human WBSCR22 is involved in cancer proliferation, invasion and metastasis; however, its function in glioma remains unexplored. In our research, we aimed to investigate the role of WBSCR22 in the development of glioma and its possible molecular mechanisms. Using bioinformatic analysis of public datasets, we determined that WBSCR22 overexpression in glioma specimens was correlated with an unfavorable patient prognosis. Our results revealed that WBSCR22 was highly expressed in glioma cell lines. The loss of WBSCR22 inhibited the growth, invasion and migration of glioma cells, while WBSCR22 overexpression produced the opposite effects. Moreover, we found that WBSCR22 downregulation reduced the phosphorylation of Akt and GSK3β and decreased the levels of β-catenin and CyclinD1 in glioma cells. The opposite effects were observed when WBSCR22 was overexpressed. Additionally, we verified with a dual-luciferase reporter assay that WBSCR22 was a direct target of miR-146b-5p. Furthermore, overexpression of miR-146b-5p suppressed WBSCR22 mRNA and protein expression. Notably, the restoration of WBSCR22 expression remarkably reversed the effects of miR-146b-5p overexpression on cell survival, apoptosis and the cell cycle in glioma cells. Collectively, our findings revealed a tumor-promoting role for WBSCR22 in glioma cells, thus providing molecular evidence for WBSCR22 as a novel therapeutic target in glioma.
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http://dx.doi.org/10.1016/j.brainresbull.2020.04.024DOI Listing
August 2020

Crowdsourcing hypothesis tests: Making transparent how design choices shape research results.

Psychol Bull 2020 05 16;146(5):451-479. Epub 2020 Jan 16.

Department of Psychology, University of the Andes.

To what extent are research results influenced by subjective decisions that scientists make as they design studies? Fifteen research teams independently designed studies to answer five original research questions related to moral judgments, negotiations, and implicit cognition. Participants from 2 separate large samples (total N > 15,000) were then randomly assigned to complete 1 version of each study. Effect sizes varied dramatically across different sets of materials designed to test the same hypothesis: Materials from different teams rendered statistically significant effects in opposite directions for 4 of 5 hypotheses, with the narrowest range in estimates being d = -0.37 to + 0.26. Meta-analysis and a Bayesian perspective on the results revealed overall support for 2 hypotheses and a lack of support for 3 hypotheses. Overall, practically none of the variability in effect sizes was attributable to the skill of the research team in designing materials, whereas considerable variability was attributable to the hypothesis being tested. In a forecasting survey, predictions of other scientists were significantly correlated with study results, both across and within hypotheses. Crowdsourced testing of research hypotheses helps reveal the true consistency of empirical support for a scientific claim. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
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http://dx.doi.org/10.1037/bul0000220DOI Listing
May 2020

Phospholipase Cγ Signaling in Bone Marrow Stem Cell and Relevant Natural Compounds Therapy.

Curr Stem Cell Res Ther 2020 ;15(7):579-587

Department of Spine Surgery, Honghui Hospital Affiliated to Xi'an Jiaotong University, Xi'an, China.

Excessive bone resorption has been recognized play a major role in the development of bone-related diseases such as osteoporosis, rheumatoid arthritis, Paget's disease of bone, and cancer. Phospholipase Cγ (PLCγ) family members PLCγ1 and PLCγ2 are critical regulators of signaling pathways downstream of growth factor receptors, integrins, and immune complexes and play a crucial role in osteoclast. Ca2+ signaling has been recognized as an essential pathway to the differentiation of osteoclasts. With growing attention and research about natural occurring compounds, the therapeutic use of natural active plant-derived products has been widely recognized in recent years. In this review, we summarized the recent research on PLCγ signaling in bone marrow stem cells and the use of several natural compounds that were proven to inhibit RANKL-mediated osteoclastogenesis via modulating PLCγ signaling pathways.
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http://dx.doi.org/10.2174/1574888X14666191107103755DOI Listing
July 2021

The Study of Natural Compounds Targeting RANKL Signaling Pathway for the Treatment of Bone Diseases.

Curr Drug Targets 2020 ;21(4):344-357

Department of Spine Surgery, Honghui Hospital Affiliated to Xi'an Jiaotong University, Xi'an, China.

Millions of people, especially for the aging people, are suffered by bone-loss diseases, such as rheumatoid arthritis (RA) and osteoporosis. Therefore, better understanding the involved mechanisms of bone metabolism is required for further treat on such diseases. Particularly, during the pathological process of bone losing, RANKL as a member of the tumor necrosis factor (TNF) superfamily, could induce osteoclast precursor cells differentiate into mature osteoclast, which grant its essential role in osteolysis. In recent years, with the increased attention paid to the natural compounds discovering studies, the therapeutic application of natural plant-derivatives have been widely recognized. Therefore, our present study aim to summarize the current novel research progressions on RANKL and its downstream signaling pathways in bone cellular differentiation, and provide a further insight for RANKL as the important drug targets in bone loss diseases. Besides that, in our current study, we also aim to briefly introduce the current application of several natural compounds for treating RANKL-mediated osteoclastic activation by modulating the RANKL signaling pathway and their therapeutic effects on the prevention of osteoporosis, osteoarthritis (OA) and RA.
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http://dx.doi.org/10.2174/1389450120666190930145144DOI Listing
February 2021

Synergistic effect of MoS and NiS nanosheets as an efficient electrocatalyst for hydrogen evolution reaction.

J Colloid Interface Sci 2019 Nov 10;556:24-32. Epub 2019 Aug 10.

Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for Nano Technology, Nanjing University, Nanjing 210093, China.

A new heterostructure [email protected] hybrid is studied as a promising candidate nonplatinum electrocatalyst for highly enhanced hydrogen evolution reaction (HER), prepared by an improved two-step hydrothermal method. Under optimized experiment conditions, the as-prepared catalysts exhibit significantly higher electrocatalytic activity than pure MoS and NiS nanosheets. The optimized [email protected] hybrid shows a small onset overpotential of 88 mV, a Tafel slope as low as 49 mV/dec, and a good durability in acidic solution. Through experimental analysis, the excellent electrocatalytic activity of HER and high stability of the hybrid largely attribute to the electrical coupling between MoS and NiS, the massive exposed active edge sites provided by layered transition metal chalcogenides (MoS), and the electrocatalytic synergistic effects produced by the [email protected] heterostructure.
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http://dx.doi.org/10.1016/j.jcis.2019.08.041DOI Listing
November 2019

miR-374a Inhibitor Enhances Etoposide-Induced Cytotoxicity Against Glioma Cells Through Upregulation of FOXO1.

Oncol Res 2019 Jun 6;27(6):703-712. Epub 2019 Mar 6.

Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, P.R. China.

Glioma is a commonly diagnosed brain tumor that shows high mortality rate. Despite the great advancement of cancer therapy in recent years, chemotherapy is still an important approach for treatment of glioma. However, long-term chemotherapy usually causes serious side effects or complications. It is desirable to take strategies to enhance the efficacy of current chemotherapy. In the present study, we observed obvious upregulation of miR-374a in glioma cells. More importantly, we found that knockdown of miR-374a was able to enhance the etoposide-induced cytotoxicity against glioma cells. Mechanically, we demonstrated that FOXO1 was the target of miR-374a in glioma. Treatment with miR-374a inhibitor induced overexpression of FOXO1, and thus promoted the expression of Bim and Noxa. Since Bim and Noxa act as key proapoptotic proteins in mitochondrial apoptosis, miR-374a inhibitor was able to enhance the etoposide-induced apoptosis pathway in glioma.
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http://dx.doi.org/10.3727/096504018X15426775024905DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848430PMC
June 2019

Flexible symmetric supercapacitor with ultrahigh energy density based on NiS/[email protected] hybrids electrode.

J Colloid Interface Sci 2019 May 18;543:147-155. Epub 2019 Feb 18.

Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for NanoTechnology, Nanjing University, Nanjing 210093, China.

A new type of 3D NiS/[email protected] reduced graphene oxide (NiS/[email protected]) hybrid composite is presented and its super-capacitive performance is studied. NiS/[email protected] hybrid is synthesized via an easy and cost-effective hydrothermal route. By combining nickel sulfide and molybdenum disulfide with N-reduced graphene oxide, the as-prepared sample demonstrates a mesoporous nanostructure that increases the accessible area of the electrolyte ions. Due to the synergistic effect in the 3D network, such NiS/[email protected] hybrid is used as electrode and it demonstrates a highly specific capacity of 2225 F/g at a current density of 1 A/g, and a good rate of 1347.3 F/g at 10 A/g. Furthermore, we also test a full symmetric supercapacitor based on NiS/[email protected], which displays excellent capacitive property up to 1028 F/g at 1 A/g, with high energy and good power density up to 35.69 W h/kg and 601.8 W/kg, and good cycle stability up to 94.5% initial capacitive retention after 50,000 loops. Moreover, the symmetric supercapacitor demonstrates excellent flexibility under different bending conditions.
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http://dx.doi.org/10.1016/j.jcis.2019.02.054DOI Listing
May 2019

Inhibition of miR-124 improves neonatal necrotizing enterocolitis via an MYPT1 and TLR9 signal regulation mechanism.

J Cell Physiol 2019 07 27;234(7):10218-10224. Epub 2018 Nov 27.

Department of Emergency Medicine, Xuzhou Children's Hospital, Xuzhou, China.

Backgrounds: Necrotizing enterocolitis (NEC) was one of the main causes of morbidity and mortality in neonates. Our objective was to detect the mechanism of miR-124 in small bowel tissues of NEC.

Methods: Hematoxylin and eosin (H&E) staining was used to detect the repair of the damaged tissues in rat NEC model. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was used to evaluate the cell apoptosis level in intestinal tissue. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) expression level of miR-124, Rho-associated coiled-coil-containing protein kinase 1 (ROCK1), myosin phosphatase target subunit 1 (MYPT1), and Toll-like receptor 9 (TLR9) in NEC tissues and IEC-6 cells. Luciferase reporter assay was used to verify whether ROCK1 is a direct target of miR-124.

Results: miR-124 was overexpressed in the NEC tissues, while ROCK1 and MYPT1 was downregulated in the NEC tissues. Inhibition of miR-124, suppressed the intestinal cell apoptosis and promoted the expression of ROCK1 and MYPT1. What is more, overexpression of miR-124 could inhibit the expression of ROCK1, TLR9, and MYPT1. Luciferase assay confirmed that miR-124 can regulate the transcriptional activity of ROCK1 through binding its 3'-UTR region.

Conclusion: miR-124 was a promoter of NEC, which promotes the intestine cell apoptosis and inflammatory cell infiltration through the inhibition of TLR9 expression by targeting ROCK1.
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http://dx.doi.org/10.1002/jcp.27691DOI Listing
July 2019

Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats.

Exp Ther Med 2018 Sep 9;16(3):2013-2021. Epub 2018 Jul 9.

Department of Gastroenterology, Nanjing Medical University, Jinling Hospital, Nanjing, Jiangsu 210002, P.R. China.

Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling constitutes one of the major pathways for cytokine signal transduction. However, the role of the JAK2/STAT3 pathway in liver injury during severe acute pancreatitis (SAP) remains unclear. The aim of this study was to investigate the role of the JAK2/STAT3 signaling pathway in liver injury after SAP. In the present study 64 male Sprague-Dawley rats were randomly divided into four groups: Control, AG490 (inhibition of JAK2), SAP and SAP with AG490. SAP was induced by retrograde infusion of 4% sodium taurocholate into the biliopancreatic duct. The activities of amylase (AMY) and liver enzymes were measured in serum. Livers and pancreas were isolated for measurements of histological damage. Blood and liver samples were taken for the measurement of TNF-α, IL-6 and IL-18 concentrations. The expression levels of JAK2 and STAT3 in liver tissue were detected by immunohistochemical staining and western blotting. The results demonstrated that amylase and liver enzymes were higher in the SAP groups compared with the control, AG490 and AG490-treated groups. The serum levels of TNF-α, IL-6 and IL-18 were effectively increased in the SAP groups, whereas they were reduced by AG490. Interestingly, JAK2 and STAT3 protein expression levels were significantly increased following induction of SAP and were significantly decreased in the AG490-pretreated groups. Administration of AG490 decreased the activity of pro-inflammatory cytokines and significantly attenuated SAP associated-liver injury in the rats. These results suggested that the mechanism may relate to the inhibition of TNF-α, IL-6 and IL-18, and inhibiting excessive JAK2 and STAT3 activation, and may play a crucial role in the liver injury associated with SAP.
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http://dx.doi.org/10.3892/etm.2018.6433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6122147PMC
September 2018

Improving antioxidant and antiproliferative activities of colla corii asini hydrolysates using ginkgo biloba extracts.

Food Sci Nutr 2018 Jun 9;6(4):765-772. Epub 2018 Mar 9.

State Key Laboratory of Chemical Resource Engineering Beijing Laboratory of Biomedical Materials Beijing University of Chemical Technology Beijing China.

Colla corii asini hydrolysates (ACCH) and ginkgo biloba extracts (EGb) possess more potent antioxidant effects when used in combination than when used alone. The mixture of ACCH and EGb at a dose ratio of 20:4(w:w) showed the highest radical scavenging activity with IC of 0.17 ± 0.01, 0.43 ± 0.02 and 1.52 ± 0.07 mg/ml against DPPH, ABTS and HO free radicals, respectively. Furthermore, the inhibition of breast cancer cells MCF-7 and MDA-MB-231 proliferation increased when these cell lines were treated with a combination of ACCH and EGb for 72 hr, with IC of 4.32 ± 0.12 mg/ml and 0.39 ± 0.01 mg/ml, respectively. The findings indicated that the mixtures of ACCH and EGb could be used to prevent and treat some diseases caused by the excessive free radicals, especially cancer. Therefore, the mixtures of ACCH and EGb might serve as a natural source of desirable antioxidant and anticancer agents for the nutraceutical and pharmaceutical industries.
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http://dx.doi.org/10.1002/fsn3.587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021696PMC
June 2018

Application of Jejunal Nutrient Tube in Congenital Duodenal Obstruction in Children.

Iran J Public Health 2018 Apr;47(4):615-617

Dept. of General Surgery, Xuzhou Children's Hospital, Xuzhou 221006, P.R. China.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996322PMC
April 2018

Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance.

Exp Ther Med 2017 Sep 12;14(3):2221-2227. Epub 2017 Jul 12.

Department of General Surgery, Children's Hospital of Soochow University, Suzhou, Jiangsu 215025, P.R. China.

The present study determined the changes in the expression levels of MYPT1, CPI-17 and MLC20 in the ileum of mice with neonatal induced necrotizing enterocolitis (NEC) to provide a basis for a pathogenesis model that includes smooth muscle changes during NEC. A group of 7-day-old BALB/c mice were fed with formula (40 µl/g, 5 times/day) and given hypoxia treatments (5% O and 95% N for 10 min, twice daily) for 4 days to induce NEC and establish a mouse model. A control group of 7-day-old BALB/c mice were left with their mother for the duration of the treatment. After establishing the model, the two groups of mice were sacrificed, and the terminal ileum tissue was collected and subjected to western blot analysis and immunohistochemistry. The results showed the expression levels of MYPT1 and pMYPT1 in the ileum of the mice in the NEC group were lower than those in the control group (P<0.01). The levels of CPI17 and pCPI17 were higher in the NEC group compared with those in the control group. The expression level of MLC20 in NEC group was lower than that in the control group (P<0.01), but the level of pMLC20 in the NEC group was higher (P<0.05). The results of immunohistochemistry showed that the staining intensities of MYPT1, CPI-17 and MLC20 in the NEC group were lighter than those in the control group, and the proportion of positive cells was also lower in the NEC group (P<0.01). Taken together our results suggest that establishment of NEC is accompanied by changes in the protein levels of MYPT1 and pCPI-17, which can regulate smooth muscle contraction in the ileum.
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http://dx.doi.org/10.3892/etm.2017.4783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609160PMC
September 2017

An efficient CoS/CoP hybrid catalyst for electrocatalytic hydrogen evolution.

Sci Rep 2017 09 19;7(1):11891. Epub 2017 Sep 19.

Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures and Jiangsu Provincial Laboratory for NanoTechnology, Nanjing University, Nanjing, 210093, China.

The development of efficient, universal and inexpensive electrocatalysts for hydrogen evolution reaction (HER) is central to the area of sustainable energy conversion. Considering the Co-based sulfides/phosphides have the same catalytic mechanism with the hydrogenases occurring in nature. Here, a new catalyst based on CoS/CoP hybrid that is comprised entirely cheap and earthabundant elements, was first synthesized via a two-step method, the Co(CO)(OH)·0.11HO precursor was prepared by a hydrothermal method, followed by phosphidation and sulphidation under Ar atmosphere simultaneously. The resulting CoS/CoP hybrid material possessed porous core-shell structure with a homogeneous element distribution and large electroactive surface area (~21.04 mF cm). More importantly, the nanostructured CoS/CoP electrode exhibits excellent HER properties in acid medium with a low onset overpotential of 34 mV, a small Tafel slope of 45 mV dec, as well as a large exchange current density of 150 μA cm. These results obtained in this study indicate that the CoS/CoP hybrid nanorod is promising replacement to the Pt-based catalysts for H production. Moreover, the synthetic method presented in this work can provide an efficient way to synthesis other nanocomposites.
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http://dx.doi.org/10.1038/s41598-017-12332-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605511PMC
September 2017

MoS-Based Mixed-Dimensional van der Waals Heterostructures: A New Platform for Excellent and Controllable Microwave-Absorption Performance.

ACS Appl Mater Interfaces 2017 Oct 20;9(39):34243-34255. Epub 2017 Sep 20.

Collaborative Innovation Center of Advanced Microstructures, National Laboratory of Solid State Microstructures, and Jiangsu Provincial Laboratory for NanoTechnology, Nanjing University , Nanjing 210093, China.

It is widely recognized that constructing multiple interface structures for enhanced interface polarization is beneficial to microwave absorption. Here, we report our work of achieving excellent microwave-absorption performance and controlling better-defined interfaces in vertically stacked two-dimensional (2D) MoS with other dimensional building blocks. The optimal reflection loss and effective absorbing bandwidth (reflection loss <-10 dB) of several mixed-dimensional van der Waals heterostructures are as follows: (i) for 2-0 type (2D MoS/zero-dimensional Ni nanoparticles), -19.7 dB and 2.92 GHz; (ii) for 2-1 type (2D MoS/one-dimensional carbon nanotubes), -47.9 dB and 5.60 GHz; and (iii) for 2-3 type (2D MoS/three-dimensional carbon layers), -69.2 dB and 4.88 GHz. As a result, by selected synthesis of different types of microstructures, we can regulate and control microwave-absorption properties in MoS mixed-dimensional van der Waals heterostructures. In addition, attributing to the better-defined interfaces generated in mixed-dimensional van der Waals heterostructures, we found an alternative strategy to improve microwave attenuation properties of 2-0, 2-1, and 2-3 samples by controlling interfacial contacts. The results indicate that mixed-dimensional van der Waals heterostructures provide a new stage for the next generation of microwave-absorbing materials.
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http://dx.doi.org/10.1021/acsami.7b10114DOI Listing
October 2017

Pd-catalyzed regioselective intramolecular direct arylation of 3-indolecarboxamides: access to spiro-indoline-3,3'-oxindoles and 5,11-dihydro-6H-indolo[3,2-c]quinolin-6-ones.

Chem Commun (Camb) 2017 Jul;53(55):7796-7799

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, P. R. China.

We report regioselective intramolecular direct C-3 and C-2 arylations of the indole rings of N-(o-bromophenyl)-3-indolecarboxamides for diastereospecific production of spiro-indoline-3,3'-oxindoles and 5,11-dihydro-6H-indolo[3,2-c]quinolin-6-ones, respectively, under different reaction conditions and possibly involving two different pathways.
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http://dx.doi.org/10.1039/c7cc02256eDOI Listing
July 2017

Antiviral efficacy of entecavir for hepatitis B virus rtA181V/T mutants.

Virol J 2017 04 4;14(1):68. Epub 2017 Apr 4.

Department of Liver Disease Center, Bayi Hospital Affiliated Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China.

Background: The amino acid substitution at position 181 of the Hepatitis B virus (HBV) polymerase is a multi-drug resistance affecting both the L-nucleoside and acyclic phosphonate nucleotide groups. Data is limited on the efficacy of entecavir (ETV) rescuing chronic hepatitis B (CHB) patients with rtA181T/V mutation.

Methods: Thirty-one patients with rtA181T/V mutation and 25 patients with rtA181T/V and rtN236T mutation were enrolled. Virological, serological and biochemical outcomes of ETV rescue therapy over 12 months in CHB patients with rtA181T/V mutation strains were investigated. All patients were treated with ETV 0.5 mg/day for 12 months and scheduled follow-up every 3 months. Patients' characteristics, laboratory tests results and clinical outcomes were collected and compared.

Results: After emergence of rtA181T/V mutant, serum HBV DNA levels increased over 4 log10 IU/mL, but the total bilirubin, alanine aminotransferase (ALT) levels raised moderately. No significant difference in baseline characteristics was observed between the rtA181T/V group and rtA181T/V + rtN236T group. After 12 months rescue therapy, total 85.7% (48/56) patients achieved HBV DNA undetectable. No significant difference in the mean reduction of serum HBV DNA and biochemical response was observed between both groups (3.59 ± 1.85 vs. 3.76 ± 2.15 log10 IU/ml; P = 0.756 and 90.3 vs. 80.0%; P = 0.272, respectively). The mean HBV DNA reduction, HBsAg and ALT levels were also similar between different rtA181T/sW172 mutations (P > 0.05). HBV DNA level is the only predictor of 12 months antiviral outcomes (odds ratio 6.723, P = 0.022).

Conclusions: The results of the present study suggested that ETV is efficient in rescuing rtA181T/V mutation CHB patients. HBV DNA level could predict viral clearance at the 12 month.
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http://dx.doi.org/10.1186/s12985-017-0739-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379510PMC
April 2017

Diastereospecific and Enantioselective Access to Dispirooxindoles from Furfurylcyclobutanols by Means of a Pd-Catalyzed Arylative Dearomatization/Ring Expansion Cascade.

Org Lett 2016 12 5;18(24):6440-6443. Epub 2016 Dec 5.

Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou 510640, P. R. China.

We report a Pd-catalyzed arylative dearomatization/ring expansion cascade of furfurylcyclobutanols that involves a spiro π-allyl palladium intermediate and affords structurally novel dispirooxindoles containing two quaternary carbon centers in good yields with high step economy, diastereospecificity, and enantioselectivity.
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http://dx.doi.org/10.1021/acs.orglett.6b03339DOI Listing
December 2016
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