Publications by authors named "Xiaobing Li"

294 Publications

An observation approach in evaluation of ozone production to precursor changes during the COVID-19 lockdown.

Atmos Environ (1994) 2021 Oct 14;262:118618. Epub 2021 Jul 14.

Institute for Environmental and Climate Research, Jinan University, Guangzhou, 511443, China.

The increase of surface ozone during the Corona Virus Disease 2019 (COVID-19) lockdown in China has aroused great concern. In this study, we combine 1.5 years of measurements for ozone, volatile organic compounds (VOCs), and nitrogen oxide (NO) at four sites to investigate the effect of COVID-19 lockdown on surface ozone in Dongguan, an industrial city in southern China. We show that the average concentrations of NO and VOCs decreased by 70%-77% and 54%-68% during the lockdown compared to pre-lockdown, respectively. Based on the source apportionment of VOCs, the contribution of industrial solvent use reduced significantly (86%-94%) during the lockdown, and climbed back slowly along with the re-opening of the industry after lockdown. A slight increase in mean ozone concentration (3%-14%) was observed during the lockdown. The rise of ozone was the combined effect of substantial increase at night (58%-91%) and small reduction in the daytime (1%-17%). These conflicting observations in ozone response between day and night to emission change call for a more detailed approach to diagnostic ozone production response with precursor changes, rather than directly comparing absolute concentrations. We propose that the ratio of daily Ox (i.e. ozone + NO) enhancement to solar radiation can provide a diagnostic parameter for ozone production response during the lockdown period. Smaller ratio of daily O (ozone + NO) enhancement to solar radiation during the lockdown were observed from the long-term measurements in Dongguan, suggesting significantly weakened photochemistry during the lockdown successfully reduces local ozone production. Our proposed approach can provide an evaluation of ozone production response to precursor changes from restrictions of social activities during COVID-19 epidemic and also other regional air quality abatement measures (e.g. public mega-events) around the globe.
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http://dx.doi.org/10.1016/j.atmosenv.2021.118618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8277545PMC
October 2021

The role of metabolic reprogramming in tubular epithelial cells during the progression of acute kidney injury.

Cell Mol Life Sci 2021 Jun 29. Epub 2021 Jun 29.

College of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, 450000, China.

Acute kidney injury (AKI) is one of the most common clinical syndromes. AKI is associated with significant morbidity and subsequent chronic kidney disease (CKD) development. Thus, it is urgent to develop a strategy to hinder AKI progression. Renal tubules are responsible for the reabsorption and secretion of various solutes and the damage to this part of the nephron is a key mediator of AKI. As we know, many common renal insults primarily target the highly metabolically active proximal tubular cells (PTCs). PTCs are the most energy-demanding cells in the kidney. The ATP that they use is mostly produced in their mitochondria by fatty acid β-oxidation (FAO). But, when PTCs face various biological stresses, FAO will shut down for a time that outlives injury. Recent studies have suggested that surviving PTCs can adapt to FAO disruption by increasing glycolysis when facing metabolic constraints, although PTCs do not perform glycolysis in a normal physiological state. Enhanced glycolysis in a short period compensates for impaired energy production and exerts partial renal-protective effects, but its long-term effect on renal function and AKI progression is not promising. Deranged FAO and enhanced glycolysis may contribute to the AKI to CKD transition through different molecular biological mechanisms. In this review, we concentrate on the recent pathological findings of AKI with regards to the metabolic reprogramming in PTCs, confirming that targeting metabolic reprogramming represents a potentially effective therapeutic strategy for the progression of AKI.
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http://dx.doi.org/10.1007/s00018-021-03892-wDOI Listing
June 2021

Arterial Infusion Chemotherapy for Neoplastic Esophagogastric Anastomotic Strictures After Esophagectomy.

Front Oncol 2021 26;11:668593. Epub 2021 May 26.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Neoplastic esophagogastric anastomotic strictures after resection of esophageal cancer is a very difficult problem in clinical practice. We aim at to investigate the safety and feasibility of arterial infusion chemotherapy in treatment of neoplastic esophagogastric anastomotic strictures after esophagectomy.

Methods: From October 2014 to December 2019, 50 patients with Neoplastic esophagogastric anastomotic strictures after resection of esophageal cancer were assessed retrospectively. Preoperative dysphagia was grade III in 34 cases and grade IV in 16 cases. Thirty-eight patients had different degrees of dyspnea before surgery Twenty-five patients had intolerable (grade IV) dyspnea and airway stenting was undertaken before surgery. Thirteen patients had tolerable dyspnea that did not require airway stenting, and preoperative dyspnea was grade III.

Results: All patients were successfully treated with arterial infusion chemotherapy, no paraplegia or death occurred. The dysphagia grade of 50 patients after AIC was compared: one case had grade I, 40 cases had grade II, and nine cases had grade III. Thirteen patients had tolerable dyspnea that did not necessitate airway stenting. Dyspnea was classified as grade I in five cases and grade II in eight cases. After 1-3 courses of AIC, 50 patients were followed up for a complete response (eight cases), partial response (28) and stable disease (14 cases). Total objective effective rate (complete response+ partial response) and disease control rate(complete response + partial response + stable disease)were 72.0% and 100.0%, respectively. The median duration of follow-up was 8.5 months. One-year survival was 46.0%.

Conclusion: Arterial infusion chemotherapy is safe and efficacious treatment for Neoplastic esophagogastric anastomotic strictures after esophagectomy.
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http://dx.doi.org/10.3389/fonc.2021.668593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187754PMC
May 2021

Behavioral pathways in bicycle-motor vehicle crashes: From contributing factors, pre-crash actions, to injury severities.

J Safety Res 2021 06 18;77:229-240. Epub 2021 Mar 18.

Alabama Transportation Institute, The University of Alabama, Tuscaloosa, AL 35487, United States. Electronic address:

Introduction: This study performed a path analysis to uncover the behavioral pathways (from contributing factors, pre-crash actions to injury severities) in bicycle-motor vehicle crashes.

Method: The analysis investigated more than 7,000 bicycle-motor vehicle crashes in North Carolina between 2007 and 2014. Pre-crash actions discussed in this study are actions of cyclists and motorists prior to the event of a crash, including "bicyclist failed to yield," "motorist failed to yield," "bicyclist overtaking motorist," and "motorist overtaking bicyclist."

Results: Model results show significant correlates of pre-crash actions and bicyclist injury severity. For example, young bicyclists (18 years old or younger) are 23.5% more likely to fail to yield to motor traffic prior to the event of a crash than elder bicyclists. The "bicyclist failed to yield" action is associated with increased bicyclist injury severity than other actions, as this behavior is associated with an increase of 5.88 percentage points in probability of a bicyclist being at least evidently injured. The path analysis can highlight contributing factors related to risky pre-crash actions that lead to severe injuries. For example, bicyclists traveling on regular vehicle travel lanes are found to be more likely to involve the "bicyclist failed to yield" action, which resulted in a total 44.38% (7.04% direct effect + 37.34% indirect effect) higher likelihood of evident or severe injuries. The path analysis can also identify factors (e.g., intersection) that are not directly but indirectly correlated with injury severity through pre-crash actions. Practical Applications: This study offers a methodological framework to quantify the behavioral pathways in bicycle-motor vehicle crashes. The findings are useful for cycling safety improvements from the perspective of bicyclist behavior, such as the educational program for cyclists.
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http://dx.doi.org/10.1016/j.jsr.2021.02.015DOI Listing
June 2021

Effect of Mg Addition on the Microstructure and Properties of a Heat-Affected Zone in Submerged Arc Welding of an Al-Killed Low Carbon Steel.

Materials (Basel) 2021 May 8;14(9). Epub 2021 May 8.

School of Metallurgy, Northeastern University, Shenyang 110819, China.

To reveal the effect of Mg treatment on the microstructure evolution behavior in the actual steel welding process, the microstructure and properties of Al-deoxidized high-strength ship plate steel with Mg addition were analyzed after double-side submerged arc welding. It was found that the Al-Mg-O + MnS inclusion formed under 26 ppm Mg treatment could promote acicular ferrite (AF) nucleation in the coarse-grained heat-affected zone (CGHAZ) and inhibit the formation of widmanstätten ferrite and coarse grain boundary ferrite. In the fine-grained heat-affected zone (FGHAZ) and intercritical heat-affected zone (ICHAZ), polygonal ferrite and pearlite were dominant. Al-Mg-O+MnS cannot play a role in inducing AF, but the grain size of ferrite was refined by Mg addition. The impact toughness in HAZ of the Mg-added steel was higher than that of Mg-free steel. With the heat-input rising from 29.55 to 44.11 kJ/cm, it remained relatively stable in Mg-treated steel. From the fusion line to the base metal, the micro-hardness of the fusion zone, CGHAZ, ICHAZ and FGHAZ decreased to some extent after Mg addition, which means the cold cracking tendency in the welding weak zone could be reduced. Finally, the mechanisms of Mg-containing inclusion-induced AF were also systematically discussed.
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http://dx.doi.org/10.3390/ma14092445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125884PMC
May 2021

Pharmacoeconomic Evaluation of Erlotinib for the Treatment of Pancreatic Cancer.

Clin Ther 2021 May 28. Epub 2021 May 28.

Department of Pharmacy, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:

Purpose: To evaluate the cost-effectiveness of gemcitabine and gemcitabine plus erlotinib as first-line treatments for advanced pancreatic cancer.

Methods: On the basis of the Gemcitabine With/Out Erlotinib in Unresectable Locally Advanced/Metastatic Pancreatic Cancer (PA.3) trial, the Markov model was constructed to simulate the development of advanced pancreatic cancer. Cost-effectiveness analysis was used to determine the economic level of the treatments, according to the willingness-to-pay (WTP) threshold. The sensitivity analysis was conducted for cost-effectiveness and other indexes.

Findings: The results of the cost-effectiveness analysis revealed that the cost-effectiveness ratios for the first-line treatment of advanced pancreatic cancer were ¥60,492.78 (US$8892.44/€7568.88) per 6.34 quality-adjusted life-months (QALMs) for gemcitabine and ¥99,595.39 (US$14,640.52/€12,461.42) per 7.02 QALMs for gemcitabine plus erlotinib. The incremental cost-effectiveness of the 2 regimens was ¥57,503.84 ($8453.06/€7194.90) per QALM, which was higher than the WTP set in this study (¥16,161 [$2375.66/€2022.07] per QALM). The results of the sensitivity analysis indicate that the analysis results were stable. Gemcitabine was more cost-effective than gemcitabine plus erlotinib.

Implications: Compared with gemcitabine, gemcitabine plus erlotinib was not cost-effective at the level of the WTP. Gemcitabine plus erlotinib therapy has no economic significance as a first-line medical treatment for pancreatic cancer.
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http://dx.doi.org/10.1016/j.clinthera.2021.04.012DOI Listing
May 2021

Boosting endoplasmic reticulum folding capacity reduces unfolded protein response activation and intracellular accumulation of human kidney anion exchanger 1 in Saccharomyces cerevisiae.

Yeast 2021 May 25. Epub 2021 May 25.

Molecular and Cell Biology, Department of Biosciences and Centre of Human and Molecular Biology (ZHMB), Saarland University, Saarbrücken, Germany.

Human kidney anion exchanger 1 (kAE1) facilitates simultaneous efflux of bicarbonate and absorption of chloride at the basolateral membrane of α-intercalated cells. In these cells, kAE1 contributes to systemic acid-base balance along with the proton pump v-H -ATPase and the cytosolic carbonic anhydrase II. Recent electron microscopy analyses in yeast demonstrate that heterologous expression of several kAE1 variants causes a massive accumulation of the anion transporter in intracellular membrane structures. Here, we examined the origin of these kAE1 aggregations in more detail. Using various biochemical techniques and advanced light and electron microscopy, we showed that accumulation of kAE1 mainly occurs in endoplasmic reticulum (ER) membranes which eventually leads to strong unfolded protein response (UPR) activation and severe growth defect in kAE1 expressing yeast cells. Furthermore, our data indicate that UPR activation is dose dependent and uncoupled from the bicarbonate transport activity. By using truncated kAE1 variants, we identified the C-terminal region of kAE1 as crucial factor for the increased ER stress level. Finally, a redistribution of ER-localized kAE1 to the cell periphery was achieved by boosting the ER folding capacity. Our findings not only demonstrate a promising strategy for preventing intracellular kAE1 accumulation and improving kAE1 plasma membrane targeting but also highlight the versatility of yeast as model to investigate kAE1-related research questions including the analysis of structural features, protein degradation and trafficking. Furthermore, our approach might be a promising strategy for future analyses to further optimize the cell surface targeting of other disease-related PM proteins, not only in yeast but also in mammalian cells.
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http://dx.doi.org/10.1002/yea.3652DOI Listing
May 2021

LncRNA PCAT18 Promotes Non-Small Cell Lung Cancer Progression by Sponging miR-4319.

Cancer Manag Res 2021 10;13:3761-3774. Epub 2021 May 10.

Department of Oncology, The People's Hospital of Xinyu City, Xinyu, Jiangxi, People's Republic of China.

Introduction: NSCLC (non-small cell lung cancer), the most common type of human cancer, is a main cause of cancer-associated mortality. Accumulating evidence has confirmed that long non-coding RNAs serve crucial roles in NSCLC development.

Methods: The PCAT18 expression in NSCLC tissues and cell lines were evaluated by reverse transcription-quantitative PCR. Cell Counting Kit-8 assays, colony formation study, wound healing assays and transwell invasion assays, and tumor xenograft experiments were performed to investigate the biological functions of PCAT18 in NSCLC. Luciferase reporter, RNA-binding protein immunoprecipitation (RIP) and RNA pull-down assays were further used to explore the association between PCAT18 and miR-4319.

Results: PCAT18 expression was up-regulated in NSCLC tissues and cell lines. Furthermore, PCAT18 silencing inhibited NSCLC cell proliferation, migration and invasion, while co-transfection with a miR-4319 inhibitor reversed these biological effects, and miR-4319 inhibited NSCLC growth in vivo. Additionally, PCAT18 silencing promoted NSCLC cell apoptosis and induced G1 stage arrest. Moreover, luciferase reporter assays illustrated that PCAT18 regulated miR-4319 directly, and a RIP assay and RNA pull-down analysis further demonstrated that miR-4319 inhibited PCAT18 in a RNA-induced silencing complex-dependent manner. Finally, PCAT18 silencing impaired the growth of NSCLC in vivo.

Conclusion: In conclusion, these findings demonstrated that PCAT18 promoted NSCLC development by sponging miR-4319. PCAT18 may serve as a crucial biomarker for the diagnosis and targeted therapy of NSCLC.
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http://dx.doi.org/10.2147/CMAR.S298918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122005PMC
May 2021

Covariation of the Fecal Microbiome with Diet in Nonpasserine Birds.

mSphere 2021 05 12;6(3). Epub 2021 May 12.

Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China

Opportunistic feeding and multiple other environment factors can modulate the gut microbiome, and bias conclusions, when wild animals are used for studying the influence of phylogeny and diet on their gut microbiomes. Here, we controlled for these other confounding factors in our investigation of the magnitude of the effect of diet on the gut microbiome assemblies of nonpasserine birds. We collected fecal samples, at one point in time, from 35 species of birds in a single zoo as well as 6 species of domestic poultry from farms in Guangzhou city to minimize the influences from interfering factors. Specifically, we describe 16S rRNA amplicon data from 129 fecal samples obtained from 41 species of birds, with additional shotgun metagenomic sequencing data generated from 16 of these individuals. Our data show that diets containing native starch increase the abundance of in the gut microbiome, while those containing plant-derived fiber mainly enrich the level of Greater numbers of and are detected in carnivorous birds, while in birds fed a commercial corn-soybean basal diet, a stronger inner-connected microbial community containing and was enriched. Furthermore, the metagenome functions of the microbes (such as lipid metabolism and amino acid synthesis) were adapted to the different food types to achieve a beneficial state for the host. In conclusion, the covariation of diet and gut microbiome identified in our study demonstrates a modulation of the gut microbiome by dietary diversity and helps us better understand how birds live based on diet-microbiome-host interactions. Our study identified food source, rather than host phylogeny, as the main factor modulating the gut microbiome diversity of nonpasserine birds, after minimizing the effects of other complex interfering factors such as weather, season, and geography. Adaptive evolution of microbes to food types formed a dietary-microbiome-host interaction reciprocal state. The covariation of diet and gut microbiome, including the response of microbiota assembly to diet in structure and function, is important for health and nutrition in animals. Our findings help resolve the major modulators of gut microbiome diversity in nonpasserine birds, which had not previously been well studied. The diet-microbe interactions and cooccurrence patterns identified in our study may be of special interest for future health assessment and conservation in birds.
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http://dx.doi.org/10.1128/mSphere.00308-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125056PMC
May 2021

Downregulation of TAF9B by miR-7-5p Inhibits the Progression of Osteosarcoma.

Onco Targets Ther 2021 30;14:2917-2927. Epub 2021 Apr 30.

Department of Orthopaedics, The Second Hospital of Shandong University, Jinan, Shandong, People's Republic of China.

Background: Osteosarcoma (OS) is a malignant bone tumor with high metastatic potential. As a regulatory factor of apoptosis, TATA-box binding protein (TBP) associated factor 9B (TAF9B) is rarely studied in tumors.

Methods: We investigated the role and mechanism of TAF9B in OS cells by overexpression and knockdown. CCK8, colony formation, transwell, and flow cytometry analysis were performed to detect proliferation, migration, invasion, and apoptosis.

Results: TAF9B overexpression promotes the proliferation, migration, and invasion of OS cells, while TAF9B knockdown gives the opposite result. TAF9B inhibits apoptosis by upregulating Bcl-2 and downregulating Bax and Cleaved-caspase-3. Through starBase analysis, it was found that miR-7-5p can bind to the 3'UTR region of TAF9B, which is further confirmed by the dual luciferase reporter system assay. MiR-7-5p downregulates the expression of TAF9B in MG63 and U2OS cells. The proliferation and invasion of OS cells are inhibited after miR-7-5p mimics transfection and are promoted after miR-7-5p inhibitor transfection. TAF9B rescues the inhibitory effect of miR-7-5p on OS cells. TAF9B also activates the AKT/mTOR signaling pathway.

Conclusion: According to our results, miR-7-5p inhibits the translation of TAF9B and then suppresses growth and metastasis through the AKT/mTOR signaling pathway in OS cells, thereby indicating the potential value of miR-7-5p and TAF9B as therapeutic targets for human OS.
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http://dx.doi.org/10.2147/OTT.S264786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096444PMC
April 2021

Which factors matter the most? Revisiting and dissecting antibody therapeutic doses.

Drug Discov Today 2021 Apr 22. Epub 2021 Apr 22.

Division of Pharmacotherapy and Experimental Therapeutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address:

Factors such as antibody clearance and target affinity can influence antibodies' effective doses for specific indications. However, these factors vary considerably across antibody classes, precluding direct and quantitative comparisons. Here, we apply a dimensionless metric, the therapeutic exposure affinity ratio (TEAR), which normalizes the therapeutic doses by antibody bioavailability, systemic clearance and target-binding property to enable direct and quantitative comparisons of therapeutic doses. Using TEAR, we revisited and dissected the doses of up to 60 approved antibodies. We failed to detect a significant influence of target baselines, turnovers or anatomical locations on antibody therapeutic doses, challenging the traditional perceptions. We highlight the importance of antibodies' modes of action for therapeutic doses and dose selections; antibodies that work through neutralizing soluble targets show higher TEARs than those working through other mechanisms. Overall, our analysis provides insights into the factors that influence antibody doses, and the factors that are crucial for antibodies' pharmacological effects.
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http://dx.doi.org/10.1016/j.drudis.2021.04.022DOI Listing
April 2021

Enforced expression of miR-92b blunts lipopolysaccharide-mediated inflammatory injury by activating the PI3K/AKT/β-catenin pathway via targeting PTEN.

Int J Biol Sci 2021 25;17(5):1289-1301. Epub 2021 Mar 25.

College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, Yunnan, People's Republic of China.

Endometritis is a reproductive disorder characterized by an inflammatory response in the endometrium, which causes significant economic losses to the dairy farming industry. MicroRNAs (miRNAs) are implicated in the inflammatory response and immune regulation following infection by pathogenic bacteria. Recent miRNA microarray analysis showed an altered expression of miR-92b in cows with endometritis. In the present study, we set out to investigate the regulatory mechanism of miR-92b in endometritis. Here, qPCR results first validated that miR-92b was down-regulated during endometritis. And then, bovine endometrial epithelial cells (BEND cells) stimulated by high concentration of lipopolysaccharide (LPS) were employed as an inflammatory injury model. Our data showed that overexpression of miR-92b significantly suppressed the activation of Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) in LPS-stimulated BEND cells, thereby reducing pro-inflammatory cytokines release and inhibiting cell apoptosis. Looking into the molecular mechanisms of regulation of inflammatory injury by miR-92b, we observed that overexpression of miR-92b restrained TLR4/NF-κB by activating the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT)/β-catenin pathway. Furthermore, the luciferase reporter assay suggested that miR-92b targeted inhibition of phosphatase and tensin homolog (PTEN), an inhibitor of the PI3K/AKT/β-catenin pathway. Importantly, experiments confirmed that up-regulation of miR-92b attenuated the pathological injury in an experimental murine model of LPS-induced endometritis. Collectively, these findings show that enforced expression of miR-92b alleviates LPS-induced inflammatory injury by activating the PI3K/AKT/β-catenin pathway via targeting PTEN, suggesting a potential application for miR-92b-based therapy to treat endometritis or other inflammatory diseases.
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http://dx.doi.org/10.7150/ijbs.56933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040465PMC
March 2021

The role of Fibrinogen-like proteins in Cancer.

Int J Biol Sci 2021 8;17(4):1079-1087. Epub 2021 Mar 8.

Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China.

Fibrinogen-associated protein (FREP) family is a family of proteins with a fibrin domain at the carboxyl terminus. Recent investigations illustrated that two members of FREP family, fibrinogen-like protein-1 (FGL1) and fibrinogen-like protein-2 (FGL2), play crucial roles in cancer by regulating the proliferation, invasion, and migration of tumor cells, or regulating the functions of immune cells in tumor microenvironment. Meanwhile, they are potential targets for medical intervention of tumor development. In this review, we discussed the structure, and the roles of FGL1 and FGL2 in tumors, especially the roles in regulating immune cell functions.
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http://dx.doi.org/10.7150/ijbs.56748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040309PMC
March 2021

Pathogenicity and transmissibility of a novel respirovirus isolated from a Malayan pangolin.

J Gen Virol 2021 04;102(4)

Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China.

The identification of SARS-CoV-2-like viruses in Malayan pangolins () has focused attention on these endangered animals and the viruses they carry. We successfully isolated a novel respirovirus from the lungs of a dead Malayan pangolin. Similar to murine respirovirus, the full-length genome of this novel virus was 15 384 nucleotides comprising six genes in the order 3'-(leader)-NP-P-M-F-HN-l-(trailer)-5'. Phylogenetic analysis revealed that this virus belongs to the genus and is most closely related to murine respirovirus. Notably, animal infection experiments indicated that the pangolin virus is highly pathogenic and transmissible in mice, with inoculated mice having variable clinical symptoms and a fatality rate of 70.37 %. The virus was found to replicate in most tissues with the exception of muscle and heart. Contact transmission of the virus was 100 % efficient, although the mice in the contact group displayed milder symptoms, with the virus mainly being detected in the trachea and lungs. The isolation of a novel respirovirus from the Malayan pangolin provides new insight into the evolution and distribution of this important group of viruses and again demonstrates the potential infectious disease threats faced by endangered pangolins.
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http://dx.doi.org/10.1099/jgv.0.001586DOI Listing
April 2021

[Analysis of genetic variants in a case with Rotor syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Apr;38(4):359-362

Department of Pediatrics, Jinhua Central Hospital, Jinhua, Zhejiang 321000, China.

Objective: To explore the genetic basis for a patient featuring Rotor syndrome.

Methods: Clinical data of the patient was collected. Whole exome sequencing (WES) based on high-throughput sequencing technology was carried out. Long-interspersed element-1 (LINE-1) insertion in intron 5 of the SLCO1B3 gene was detected by using tri-primer single tube PCR.

Results: WES revealed that the patient has carried homozygous c.1738C>T nonsense variants of the SLCO1B1 gene. He was also found to harbor a homozygous insertion of LINE-1 in intron 5 of the SLCO1B3 gene, which has caused skipping of exon 5 or exons 5 to 7 and introduced a stop codon in the SLCO1B3 transcript.

Conclusion: The homozygous c.1738C>T variant of the SLCO1B1 gene and homozygous insertion of LINE-1 in intron 5 of the SLCO1B3 gene probably underlay the Rotor syndrome in this patient.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200131-00051DOI Listing
April 2021

A new discovered gene mutation in a child with dilated cardiomyopathy.

Cardiol Young 2021 Apr 5:1-2. Epub 2021 Apr 5.

Department of Cardiothoracic Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University, Putuo District, Shanghai, China.

Dilated cardiomyopathy is characterised by dilatation and impaired contraction of the left ventricle or both ventricles, which is the most common childhood cardiomyopathy. In recent years, it has been recognised that many sorts of genetic mutations may contribute to dilated cardiomyopathy. We now report a rare association of dilated cardiomyopathy with site mutation of BMPR2 gene. We did not find such an association reported in the medical literature.
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http://dx.doi.org/10.1017/S1047951121001104DOI Listing
April 2021

Serum Protein N-Glycosylation Signatures of Neuroblastoma.

Front Oncol 2021 16;11:603417. Epub 2021 Mar 16.

Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: Neuroblastoma is the most common extracranial childhood solid tumor which accounts for 10% of the malignancies and 15% of the cancer fatalities in children. N-glycosylation is one of the most frequent post-translation protein modification playing a vital role in numerous cancers. N-glycosylation changes in neuroblastoma patient serum have not been studied in existing reports. The comprehensive analyses of serum N-glycomics in neuroblastoma can provide useful information of potential disease biomarkers and new insights of the pathophysiology in neuroblastoma.

Methods: The total serum protein N-glycosylation was analyzed in 33 neuroblastoma patients and 40 age- and sex-matched non-malignant controls. N-glycans were enzymatically released, derivatized to discriminate linkage-specific sialic acid, purified by HILIC-SPE, and identified by MALDI-TOF-MS. Peak areas were acquired by the software of MALDI-MS sample acquisition, processed and analyzed by the software of Progenesis MALDI.

Results: Three glyco-subclasses and six individual N-glycans were significantly changed in neuroblastoma patients compared with controls. The decreased levels of high mannose N-glycans, hybrid N-glycans, and increased levels of α2,3-sialylated N-glycans, multi-branched sialylated N-glycans were observed in neuroblastoma patients. what is more, a glycan panel combining those six individual N-glycans showed a strong discrimination performance, with an AUC value of 0.8477.

Conclusions: This study provides new insights into N-glycosylation characteristics in neuroblastoma patient serum. The analyses of total serum protein N-glycosylation could discriminate neuroblastoma patients from non-malignant controls. The alterations of the N-glycomics may play a suggestive role for neuroblastoma diagnosis and advance our understanding of the pathophysiology in neuroblastoma.
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http://dx.doi.org/10.3389/fonc.2021.603417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8008057PMC
March 2021

Author's response.

Am J Orthod Dentofacial Orthop 2021 04;159(4):410

Chengdu, Sichuan, China.

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http://dx.doi.org/10.1016/j.ajodo.2020.11.015DOI Listing
April 2021

COVID-19 effects on shared-biking in New York, Boston, and Chicago.

Transp Res Interdiscip Perspect 2021 Mar 15;9:100282. Epub 2020 Dec 15.

Alabama Transportation Institute, The University of Alabama, Tuscaloosa, AL 35487, United States.

Coronavirus has had a large-scale impact on transportation. This study attempts to assess the effects of COVID-19 on biking. Bikeshare data was used to understand the impacts of COVID-19 during the initial wave of the disease on biking in New York City, Boston, and Chicago. As the cases increased, these cities experienced a reduction in bikeshare trips, and the reductions were different in the three cities. Correlations were developed between COVID-19 cases and various bikeshare related variables. The study period was split into three phases-no COVID-19 phase, cases increasing phase, and cases decreasing phase-to examine how the residents of the three cities reacted during the different phases of the coronavirus spread. While bike trips decreased, the average duration of the trips increased during the pandemic. NYC's average trip duration was consistently less than that of Boston and Chicago, which could be due to its sprawl (NYC is considered as more compact and connected compared to the other two cities).
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http://dx.doi.org/10.1016/j.trip.2020.100282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7964246PMC
March 2021

Inhibition of cell death inducing DNA fragmentation factor-α-like effector c (CIDEC) by tumor necrosis factor-α induces lipolysis and inflammation in calf adipocytes.

J Dairy Sci 2021 May 6;104(5):6134-6145. Epub 2021 Mar 6.

Key Laboratory of Zoonoses Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin Province 130062, China. Electronic address:

Dairy cows with ketosis exhibit signs of pronounced adipose tissue lipolysis and systemic inflammation, both of which exacerbate this metabolic disorder. In nonruminants, CIDEC plays a pivotal role in the formation of large unilocular lipid droplets. The present study aimed to ascertain the role of CIDEC in the lipolytic and inflammatory response of white adipose tissue (WAT) in vivo and in vitro. Subcutaneous adipose tissue and blood samples were collected from 15 healthy cows (blood β-hydroxybutyrate concentration < 1.2 mM) and 15 cows with clinical ketosis (blood β-hydroxybutyrate concentration > 3.0 mM) that had a similar number of lactations (median = 3, range = 2-4) and days in milk (median = 6 d, range = 3-9). Adipocytes isolated from 5 healthy Holstein calves (1 d old, female, 30-40 kg) were used for in vitro studies. Isolated adipocytes were treated with 0, 0.1, 1, or 10 ng/mL TNF-α for 3 h, transfected with CIDEC small interfering RNA for 48 h, or transfected with CIDEC overexpression adenovirus for 48 h followed by treatment with TNF-α (0.1 ng/mL) for 3 h. Serum concentrations of fatty acids were greater, and dry matter intake, milk yield, and serum glucose concentrations lower in cows with clinical ketosis. Protein and mRNA abundance of CIDEC were lesser in subcutaneous WAT of clinically ketotic versus healthy cows. Furthermore, the ratio of phosphorylated hormone sensitive lipase (p-LIPE) to LIPE, phosphorylated RELA (p-RELA) to RELA, and protein abundance of PNPLA2 and phosphorylated inhibitor of κBα (p-NFKBIA) were greater in dairy cows with clinical ketosis. The mRNA abundance of proinflammatory cytokines TNFA and IL1B were greater, and the anti-inflammatory cytokine IL10 was lower in WAT of dairy cows with clinical ketosis. In calf adipocytes, exogenous TNF-α (0.1, 1, or 10 ng/mL) decreased protein and mRNA abundance of CIDEC. In addition, exogenous TNF-α or knockdown of CIDEC reduced the secretion of the anti-inflammatory cytokine IL-10, but increased the ratio of p-LIPE to LIPE, p-RELA to RELA, protein abundance of PNPLA2 and p-NFKBIA, glycerol content, and the secretion of IL-1β in calf adipocytes. Overexpression of CIDEC in TNFα-treated adipocytes attenuated lipolysis and activation of the NF-κB signaling pathway. Overall, these data suggest that inhibition of lipid droplet-associated protein CIDEC by TNF-α contributes to the pronounced lipolysis and inflammation of calf adipocytes, and CIDEC is a relevant target in clinically ketotic cows.
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http://dx.doi.org/10.3168/jds.2020-19319DOI Listing
May 2021

MicroRNA-30a Modulates Type I Interferon Responses to Facilitate Coxsackievirus B3 Replication Targeting Tripartite Motif Protein 25.

Front Immunol 2020 14;11:603437. Epub 2021 Jan 14.

Department of Cardiothoracic Surgery, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai, China.

Viral myocarditis is caused by a viral infection and characterized by the inflammation of the myocardium. Coxsackievirus B3 (CVB3) infection is one of the most common among the infections caused by this virus. The host's early innate immune response to CVB3 infection particularly depends on the functions of type I interferons (IFNs). In this study, we report that a host microRNA, miR-30a, was upregulated by CVB3 to facilitate its replication. We demonstrated that miR-30a was a potent negative regulator of IFN-I signaling by targeting tripartite motif protein 25 (TRIM25). In addition, we found that TRIM25 overexpression significantly suppressed CVB3 replication, whereas knockdown increased viral titer and VP1 protein expression. MiR-30a inhibits the expression of TRIM25 and TRIM25-mediated retinoic acid-inducible gene (RIG)-I ubiquitination to suppress IFN-β activation and production, thereby resulting in the enhancement of CVB3 replication. These results indicate the proviral role of miR-30a in modulating CVB3 infection for the first time. This not only provides a new strategy followed by CVB3 in order to modulate IFN-I-mediated antiviral immune responses by engaging host miR-30a but also improves our understanding of its pathogenesis.
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http://dx.doi.org/10.3389/fimmu.2020.603437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7840606PMC
June 2021

Radiation induces stress and transgenerational impacts in the cricket, .

Int J Radiat Biol 2021 Jan 20:1-11. Epub 2021 Jan 20.

Department of Biology, McMaster University, Hamilton, Canada.

Purpose: Radiation exposure of crickets during their fourth juvenile molt inflicted ionizing radiation damage and altered growth rate, adult size at sexual maturity. High levels of ionizing radiation also impacted the subsequent generation, likely via heritable epigenetic mechanisms. Using radiation as a proxy for external stress, we aim to understand the transgenerational impacts of stress on non-irradiated offspring.

Methods And Materials: We assess the impacts of ionizing radiation on maturation mass and growth rate in F0 male and female house crickets (). We also assessed trans-generational impacts of irradiation on growth rate and maturation mass on non-irradiated offspring of irradiated parents compared to non-irradiated controls.

Results: Early-life exposure to high levels of ionizing radiationinduced lower growth rate and maturation mass compared to controls ( < .0001). Non-irradiated male F1 offspring of irradiated parents demonstrated significantly lower mass at maturation ( = .0012) and significantly faster time of maturation ( < .0001) compared to F1 non-irradiated controls.

Conclusion: Our results show that a single early-life exposure to ionizing radiation can alter male offspring development through accelerated maturation and reduced maturation mass.
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http://dx.doi.org/10.1080/09553002.2021.1872816DOI Listing
January 2021

Visual multiple cross displacement amplification for the rapid identification of S. agalactiae immediately from vaginal and rectal swabs.

AMB Express 2021 Jan 6;11(1). Epub 2021 Jan 6.

Department of Respiratory and Critical Medical, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Streptococcus agalactiae (S. agalactiae) is an important pathogen that can lead to neonatus and mother infection. The current existing techniques for the identification of S. agalactiae are limited by accuracy, speed and high-cost. Therefore, a new multiple cross displacement amplification (MCDA) assay was developed for test of the target pathogen immediately from vaginal and rectal swabs. MCDA primers screening were conducted targeting S. agalactiae pcsB gene, and one set of MCDA primers with better rapidity and efficiency was selected for establishing the S. agalactiae-MCDA assay. As a result, the MCDA method could be completed at a constant temperature of 61 °C, without the requirement of special equipment. The detection limit is 250 fg (31.5 copies) per reaction, all S. agalactiae strains displayed positive results, but not for non-S. agalactiae strains. The visual MCDA assay detected 16 positive samples from 200 clinical specimen, which were also detected positive by enrichment/qPCR. While the CHROMagar culture detected 6 positive samples. Thus, the MCDA assay is prefer to enrichment/qPCR and culture for detecting S. agalactiae from clinical specimen. Particularly, the whole test of MCDA takes about 63.1 min, including sample collection (3 min), DNA preparation (15 min), MCDA reaction (45 min) and result reporting (6 s). In addition, the cost was very economic, with only US$ 4.9. These results indicated that our S. agalaciae-MCDA assay is a rapid, sensitive and cost-efficient technique for target pathogen detection, and is more suitable than conventional assays for an urgent detection, especially for 'on-site' laboratories and resource-constrained settings.
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http://dx.doi.org/10.1186/s13568-020-01168-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788142PMC
January 2021

Long Non-Coding RNAs: Potential Biomarkers and Targets for Hepatocellular Carcinoma Therapy and Diagnosis.

Int J Biol Sci 2021 1;17(1):220-235. Epub 2021 Jan 1.

Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, Yunnan, China.

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related death worldwide. Increasing studies showed that long non-coding RNAs (lncRNAs), a novel class of RNAs that are greater than 200 nucleotides in length but lack the ability to encode proteins, exert crucial roles in the occurrence and progression of HCC. LncRNAs promote the proliferation, migration, invasion, autophagy, and apoptosis of tumor cells by regulating downstream target gene expression and cancer-related signaling pathways. Meanwhile, lncRNA can be used as biomarkers to predict the efficacy of HCC treatment strategies, such as surgery, radiotherapy, chemotherapy, and immunotherapy, and as a potential individualized tool for HCC diagnosis and treatment. In this review, we overview up-to-date findings on lncRNAs as potential biomarkers for HCC surgery, radiotherapy, chemotherapy resistance, target therapy, and immunotherapy, and discuss the potential clinical application of lncRNA as tools for HCC diagnosis and treatment.
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http://dx.doi.org/10.7150/ijbs.50730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757045PMC
January 2021

A spatiotemporal analysis of motorcyclist injury severity: Findings from 20 years of crash data from Pennsylvania.

Accid Anal Prev 2021 Mar 30;151:105952. Epub 2020 Dec 30.

Alabama Transportation Institute, Department of Civil, Construction, and Environmental Engineering, 248 Kirkbride Lane, 3024 Cyber Hall, The University of Alabama, Tuscaloosa, AL, 35487, United States. Electronic address:

Motorcyclists face higher risks of severe injuries in crashes compared to motor vehicle drivers who are often protected by seatbelts and airbags during collisions. A report by the National Highway Traffic Safety Administration reveals that motorcyclists have 27 times the risk of fatality in traffic crashes as much as motor vehicle drivers. Previous studies have identified a list of risk factors associated with motorcyclist injury severity and generated valuable insights for countermeasures to protect motorcyclists in crashes. These studies have shown that wearing helmets and/or motorcycle-specific reflective clothing and boots, driving alcohol/drug-free, and obeying traffic regulations are good practices for safe motorcycling. However, these practices and other risk factors are likely to interact with local geographic, socio-economic, and cultural contexts, leading to diversified correlations with motorcyclist injury severity, which remains under-explored. Such correlations may exhibit variations across space and time. The objective of this study is to revisit the correlates of motorcyclist injury severity with a focus on the spatial and temporal variations of correlations between risk factors and injury severity. This study employed an integrated spatiotemporal analytical approach to mine comprehensive statewide 20 years' motorcycle-involved traffic crashes (N = 50,823) in Pennsylvania. Non-stationarity tests were performed to examine the significance of variations in spatially and temporally local correlations. The results show that most factors, such as helmet, engine size, vehicle age, pillion passenger, at-fault striking, and speeding, hold significant non-stationary relationships with motorcyclist injury severity. Furthermore, cluster analysis of estimations reveals the regional similarities of correlates, which may help practitioners develop regional motorcyclist safety countermeasures.
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http://dx.doi.org/10.1016/j.aap.2020.105952DOI Listing
March 2021

A novel mutation in leads to primary familial brain calcification and cerebral infarction.

Int J Neurosci 2021 Jan 24:1-5. Epub 2021 Jan 24.

Department of Neurology, the First Affiliated Hospital of Nanchang University, Nanchang, China.

Primary familial brain calcification (PFBC) is a rare inherited disorder characterized by bilateral calcification mainly in the basal ganglia, thalamus, and cerebellar nuclei. Recently, the gene, as the first autosomal recessive causal gene for PFBC, was reported in six unrelated Chinese families. Patients with PFBC rarely present with cerebrovascular disease. Here, we report a young patient with PFBC who carried a novel homozygous mutation in the gene presenting with cerebral infarction involving the posterior limb of the right internal capsule. Brain computed tomography (CT) demonstrated symmetric calcifications in the basal ganglia, thalamus, midbrain, pons, cerebellum and frontal lobes. We found one homozygous mutation in the gene (NM_020702.3 exon2: c.830delC; p.P277Qfs*3) in this patient by Sanger sequencing. Currently, the association of PFBC and cerebral infarction, as well as the physiological role of the gene, is not clear and worth special attention and further investigation.
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http://dx.doi.org/10.1080/00207454.2020.1869000DOI Listing
January 2021

Potential hemo-biological identification markers to the left displaced abomasum in dairy cows.

BMC Vet Res 2020 Dec 2;16(1):470. Epub 2020 Dec 2.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Jilin, 130062, Changchun, China.

Background: Left displaced abomasum (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential applicability of blood biomarkers for early warning and diagnosis of LDA in dairy cows.

Results: Twenty early postpartum healthy cows and thirty early postpartum LDA cows of similar parity were used. A receiver operating characteristic curve (ROC) method was used to analyze the sensitivity of hematological biomarkers to LDA including energy balance metabolic biomarkers, liver/kidney function biomarkers, and minerals. A cut-off point was defined for each of the selected hematological biomarkers deemed sensitive markers of LDA. Compared with healthy cows, body condition score (BCS), dry matter intake (DMI) and milk production were lower in LDA cows. Among energy metabolism markers, serum non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), insulin (INS), and revised quantitative insulin sensitivity check index (RQUICKI) levels were lower while serum glucagon (GC) was greater in LDA cows. Among the liver/kidney function biomarkers, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), the ratio of AST/ALT and levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), albumin (ALB), blood urea nitrogen (BUN), creatinine, and total protein (TP) were greater in LDA cows. Among minerals analyzed, serum Cl, Ca, and K were lower in LDA cows. After ROC analysis, it was determined that serum Ca, INS, RQUICKI, ALT, GGT, and creatinine are potential indicators for early warning and diagnosis of LDA for early postpartum dairy cows.

Conclusions: Dairy cows with LDA were under severe negative energy balance (NEB), had signs of liver damage and potentially lower insulin sensitivity. A combination of multi-hematological biomarkers including Ca, INS, RQUICKI, ALT, GGT and creatinine has the potential to help identify cows at risk of LDA in the early postpartum period.
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http://dx.doi.org/10.1186/s12917-020-02676-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709353PMC
December 2020

Soil wind erosion evaluation and sustainable management of typical steppe in Inner Mongolia, China.

J Environ Manage 2021 Jan 15;277:111488. Epub 2020 Oct 15.

School of Natural Resources, Faculty of Geographical Science, Beijing Normal University, Beijing, 100875, China; State Key Laboratory of Earth Surface Process and Resource Ecology, Beijing Normal University, Beijing, 100875, China. Electronic address:

Soil wind erosion is an important ecological environmental problem that is widespread in arid and semi-arid regions. Currently, related studies are mainly focused on spatiotemporal characteristics or analysis of effector mechanisms, and they do not facilitate direct servicing of management decisions. In this paper, we used the Xilingol typical steppe in Inner Mongolia, China, as a study site to develop a decision framework for a comprehensive understanding of soil wind erosion and to promote sustainable management of steppes. In this study, we used the Revised Wind Erosion Equation model to simulate soil wind erosion. We combined this model with linear trend analysis to evaluate the ecological effects of soil wind erosion and wind erosion intensity, and delineated the gravity center migration path. We used the constraint line method to reveal the mechanisms by which climatic factors affected soil wind erosion, achieved the spatial visualization of wind-breaking and sand-fixing service flow, and proposed decision-based regional sustainable development suggestions. The results showed that long-term soil wind erosion will lead to soil coarsening and loss of soil nutrients. Soil wind erosion in the study site showed worsening trends and risks, and as such, ecological environment conservation and management are urgently required. The study framework promotes a clear understanding of the complex relationship of human-earth systems. The study results will aid in the ecological remediation of steppe landscapes and the prevention of desertification and will provide a foundation for win-win management of ecological conservation and economic development in arid and semi-arid regions.
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http://dx.doi.org/10.1016/j.jenvman.2020.111488DOI Listing
January 2021

LncRNA FOXD3-AS1 knockdown protects against cerebral ischemia/reperfusion injury via miR-765/BCL2L13 axis.

Biomed Pharmacother 2020 Dec 14;132:110778. Epub 2020 Oct 14.

Department of Neurology, First Affiliated Hospital Nanchang University, Jiangxi Province, 330006, PR China. Electronic address:

Aims: Long non-coding RNAs (lncRNAs) FOXD3-AS1 was reported to be increased in cardiomyocyte ischemic injury. However, its role and underlying molecular mechanism in ischemic stroke remain unknown. This study was to investigate the role of FOXD3-AS1 in cerebral ischemia/reperfusion injury.

Methods: The expression of FOXD3-AS1 and miR-765 were measured with qRT-PCR. The shared putative miR-765 binding sites both in BCL2L13 and FOXD3-AS1 were identified with bioinformatics, luciferase reporter assay and RNA immunoprecipitation. Apoptosis and its related proteins were detected by TUNEL assay, Hoechst 33,258 staining, flow cytometry and western blot. Infarct volume and the neurological status were evaluated with TTC staining and neurologic deficit score, respectively.

Results: The up-regulation of FOXD3-AS1 and down-regulation of miR-765 were found in both mouse brains after cerebral ischemia/reperfusion (I/R) and neuroblastoma cells of neuro-2A (N2a) after oxygen-glucose deprivation/reoxygenation (OGD/R). Moreover, the overexpression of miR-765 reduced N2a cell apoptosis caused by OGD/R. MiR-765 could target BCL2L13 directly. In addition, we found that FOXD3-AS1 bound to miR-765 directly, acting as a ceRNA to modulate the expression of BCL2L13. Overexpression of FOXD3-AS1 antagonized the inhibitory impact of miR-765 on the expression of BCL2L13 and the apoptosis of N2a cells treated with OGD/R, while FOXD3-AS1 knockdown promoted the inhibitory impact of miR-765 on the expression of BCL2L13 and the apoptosis of N2a cells treated with OGD/R. Furthermore, we found that neurological deficits and brain injury induced by I/R in vivo were attenuated by FOXD3-AS1 knockdown.

Conclusions: We verified a critical signaling pathway of FOXD3-AS1/miR-765/BCL2L13 in regulating cerebral ischemia/reperfusion injury.
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http://dx.doi.org/10.1016/j.biopha.2020.110778DOI Listing
December 2020

Underlying mechanism on source-sink carbon balance of grazed perennial grass during regrowth: Insights into optimal grazing regimes of restoration of degraded grasslands in a temperate steppe.

J Environ Manage 2021 Jan 6;277:111439. Epub 2020 Oct 6.

Key Laboratory of Surface Processes and Resource Ecology, Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, No. 19 Xinjiekouwai Street, Haidian District, Beijing Normal University, Beijing, 100875, China. Electronic address:

Overgrazing is the main driver of grassland degradation and productivity reduction in northern China. The restoration of degraded grasslands depends on optimal grazing regimes that modify the source-sink balance to promote best carbon (C) assimilation and allocation, thereby promoting rapid compensatory growth of the grazed plants. We used in situCO labeling and field regrowth studies of Stipa grandis P.A. Smirn.to examine the effects of different grazing intensities (light, medium, heavy, and grazing exclusion) on photosynthetic C assimilation and partitioning, on reallocation of non-structural carbohydrates during regrowth, and on the underlying regulatory mechanisms. Light grazing increased the sink demand of newly expanded leaves and significantly promoted C fixation by increasing the photosynthetic capacity of the leaves and accelerating fructose transfer from the stem. Although C assimilation decreased under medium and heavy grazing, S. grandis exhibited a tolerance strategy that preferentially allocated more starch and C to the roots for storage to balance sink competition between newly expanded leaves and the roots. Sucrose phosphate synthase (SPS), sucrose synthase (SS), and other plant hormones regulated source-sink imbalances during regrowth. Abscisic acid promoted accumulation of aboveground biomass by stimulating stem SPS activity, whereas jasmonate increased root starch synthesis, thereby increasing belowground biomass. Overall, S. grandis could optimize source-sink relationships and above- and belowground C allocation to support regrowth after grazing by the regulating activities of SPS, SS and other hormones. These results provide new insights into C budgets under grazing and guidance for sustainable grazing management in semi-arid grasslands.
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http://dx.doi.org/10.1016/j.jenvman.2020.111439DOI Listing
January 2021
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