Publications by authors named "Xiaobin Zhang"

199 Publications

Circ_0091579 exerts an oncogenic role in hepatocellular carcinoma via mediating miR-136-5p/TRIM27.

Biomed J 2021 Dec 30. Epub 2021 Dec 30.

Department of Hepatobiliary Surgery, Dongying People's Hospital, Dongying 257091, China. Electronic address:

Background: Circular RNAs (circRNAs) act as crucial regulators in tumorigenesis. In this study, the working mechanism of circ_0091579 in hepatocellular carcinoma (HCC) progression was investigated.

Methods: The expression of RNA and protein was measured via RT-qPCR and western blot assay. Cell proliferation ability was analyzed via CCK8, EdU and colony formation assays. Cell migration and invasion abilities were detected via transwell assays. Flow cytometry was applied to assess cell cycle and apoptosis. The target relation between miR-136-5p and circ_0091579 or tripartite motif containing 27 (TRIM27) was certified using dual-luciferase reporter assay. Xenograft tumor model was utilized to assess the role of circ_0091579 in tumor growth in vivo. The protein level of Ki67 in tumor tissues was analyzed by immunohistochemistry (IHC) assay.

Results: Circ_0091579 expression was elevated in HCC tissues and cell lines. HCC patients with high circ_0091579 expression displayed low survival rate. Circ_0091579 knockdown suppressed the proliferation, migration, invasion, cell cycle progression and epithelial-mesenchymal transition (EMT) and induced apoptosis of HCC cells. Circ_0091579 acted as a molecular sponge for miR-136-5p, and circ_0091579 silencing-mediated effects were largely overturned by the knockdown of miR-136-5p in HCC cells. MiR-136-5p interacted with the 3' untranslated region (3'UTR) of TRIM27, and TRIM27 overexpression largely counteracted miR-136-5p overexpression-induced influences in HCC cells. Circ_0091579 sponged miR-136-5p to up-regulate TRIM27 expression in HCC cells. Circ_0091579 silencing suppressed xenograft tumor growth in vivo.

Conclusion: Circ_0091579 exhibited an oncogenic role to enhance the malignant potential of HCC cells through mediating miR-136-5p/TRIM27 axis in vitro and in vivo.
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http://dx.doi.org/10.1016/j.bj.2021.12.009DOI Listing
December 2021

Prevalence of and risk factors for depressive and anxiety symptoms in a large sample of Chinese adolescents in the post-COVID-19 era.

Child Adolesc Psychiatry Ment Health 2021 Dec 27;15(1):80. Epub 2021 Dec 27.

Science and Education Section, The Fourth People's Hospital of Lianyungang, The Affiliated KangDa College of Nanjing Medical University, Lianyungang, 222003, People's Republic of China.

Background: Depressive and anxiety symptoms are widespread among adolescents today, creating a large social problem. However, few previous studies have addressed depression and anxiety among adolescents in Chinese cohorts. The aim of this study was to evaluate the prevalence of and risk factors for depressive and anxiety symptoms among Chinese middle school adolescent students in the post-COVID-19 era.

Methods: A total of 22,380 middle school students from Jiangsu Province were surveyed online, and their general demographic data were collected. The Patient Health Questionnaire-9 (PHQ-9) was used to assess depressive symptoms, and the seven-item Generalized Anxiety Disorder (GAD-7) scale was used to measure anxiety symptoms.

Results: Of these participants (aged 12-17 years), 25.6% had depressive symptoms, 26.9% had anxiety symptoms, and 20.6% had a combination of depression and anxiety symptoms. The prevalence of depressive symptoms was higher in female adolescents (27.6%) than in male adolescents (23.7%; χ = 45.479, P = 0.000), and the proportion with anxiety symptoms was higher among female adolescents (28.6%) than among male adolescents (25.4%; χ = 29.390, P = 0.000). Furthermore, binary logistic regression analysis showed that gender, region, and parental relationship were significantly associated with depressive symptoms among adolescents, while age, gender, region, and parental relationship were significantly associated with anxiety symptoms.

Conclusions: Our findings demonstrated that the prevalence of reported depressive and anxiety symptoms in Chinese adolescents are high. Female gender, urban region, and poor parental relationship may be risk factors for depressive and anxiety symptoms. Furthermore, policy makers, schools, and families need to pay more attention to the psychological health of adolescents, develop response plans and take early intervention measures to reduce the prevalence of adolescent depression and anxiety.
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http://dx.doi.org/10.1186/s13034-021-00429-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710930PMC
December 2021

Metabolomic and Proteomic Analyses of Persistent Valvular Atrial Fibrillation and Non-Valvular Atrial Fibrillation.

Front Genet 2021 30;12:789485. Epub 2021 Nov 30.

Department of Cardiovascular Surgery, Jiangsu Province Hospital, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Atrial fibrillation (AF) is an abnormal heart rhythm related to an increased risk of heart failure, dementia, and stroke. The distinction between valvular and non-valvular AF remains a debate. In this study, proteomics and metabolomics were integrated to describe the dysregulated metabolites and proteins of AF patients relative to sinus rhythm (SR) patients. Totally 47 up-regulated and 41 down-regulated proteins in valvular AF, and 59 up-regulated and 149 down-regulated proteins in non-valvular AF were recognized in comparison to SR patients. Moreover, 58 up-regulated and 49 significantly down-regulated metabolites in valvular AF, and 47 up-regulated and 122 down-regulated metabolites in persistent non-valvular AF patients were identified in comparison to SR patients. Based on analysis of differential levels of metabolites and proteins, 15 up-regulated and 22 down-regulated proteins, and 13 up-regulated and 122 down-regulated metabolites in persistent non-valvular AF were identified relative to valvular AF. KEGG pathway enrichment analysis showed the altered proteins and metabolites were significantly related to multiple metabolic pathways, such as Glycolysis/Gluconeogenesis. Interestingly, the enrichment pathways related to non-valvular AF were obviously different from those in valvular AF. For example, valvular AF was significantly related to Glycolysis/Gluconeogenesis, but non-valvular AF was more related to Citrate cycle (TCA cycle). Correlation analysis between the differentially expressed proteins and metabolites was also performed. Several hub proteins with metabolites were identified in valvular AF and non-valvular AF. For example, Taurine, D-Threitol, L-Rhamnose, and DL-lactate played crucial roles in valvular AF, while Glycerol-3-phosphate dehydrogenase, Inorganic pyrophosphatase 2, Hydroxymethylglutaryl-CoAlyase, and Deoxyuridine 5-triphosphate nucleotidohydrolase were crucial in non-valvular AF. Then two hub networks were recognized as potential biomarkers, which can effectively distinguish valvular AF and non-valvular persistent AF from SR samples, with areas under curve of 0.75 and 0.707, respectively. Hence, these metabolites and proteins can be used as potential clinical molecular markers to discriminate two types of AF from SR samples. In summary, this study provides novel insights to understanding the mechanisms of AF progression and identifying novel biomarkers for prognosis of non-valvular AF and valvular AF by using metabolomics and proteomics analyses.
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http://dx.doi.org/10.3389/fgene.2021.789485DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669813PMC
November 2021

Effect of nano-silica coating combined with pressurized Ar treatment on postharvest quality and reactive oxygen species metabolism in sweet cherry fruit.

Food Chem 2022 Apr 1;374:131715. Epub 2021 Dec 1.

Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address:

The mechanisms of effect of nano-silica coating and pressurized Ar on regulating reactive oxygen species (ROS) generation and scavenging in the senescence of sweet cherries remains unclear. The amounts of reactive oxygen species, hydrogen peroxide, non-enzymatic antioxidants and antioxidases, and cell membrane lipid peroxidation in sweet cherries were determined. Nano-silica coating, pressurized Ar, and the combination of these two treatments, all significantly delayed senescence by suppressing decay rate, and maintained good sensory quality. In additional, all treatments inhibited the generation and accumulation of superoxide anion and hydrogen peroxide, and mitigated the lipid peroxidation of cell membranes of sweet cherries. The combination of these two treatments maintained higher contents of ascorbic acid and glutathione, and enhanced the activities of antioxidases in sweet cherries. It is suggested that nano-silica coating and pressure Ar mediated the ROS metabolism, which might have a role in retaining the quality sweet cherries during cold storage.
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http://dx.doi.org/10.1016/j.foodchem.2021.131715DOI Listing
April 2022

Structural family factors and bullying at school: a large scale investigation based on a Chinese adolescent sample.

BMC Public Health 2021 Dec 11;21(1):2249. Epub 2021 Dec 11.

Suzhou Guangji Hospital, 11# Guangqian Road, Suzhou, 215137, China.

Backgrounds: Various family factors have been identified in association with school bullying and the involvement of children and adolescents in bullying behaviors.

Methods: A total of 11,919 participants (female = 6671, mean age = 15) from 22 middle schools in Suzhou City, China completed the questionnaire. The associations between structural family factors (family socio-economic status, living arrangement, number of siblings, whether they were local residents/migrants, had an urban/rural hukou [a household registration system in China], parental and maternal education levels, and other various bullying-related constructs (i.e. bullying witnessing, bullying involvement, bystander intervention, and fear of being bullied) were all examined. Odds ratios (ORs) adjusted for covariates were calculated for the four bullying-related constructs (bullying witness, bullying involvement, bystander intervention, and reactions to being bullied) using structural family factors.

Results: The result showed that all demographic household characteristics were associated with bullying at school except for being from a single-child family. Adolescents from rural families witnessed more bullying incidents than those from local families (OR = 1.35, 95% CI: [1.09, 1.68]). Adolescents who come from migrant families (OR = 1.12, 95% CI: [1.07, 1.43]) with a rural hukou (OR = 1.31, 95% CI: [1.00, 1.74]) and low parental education levels (OR = 1.42, 95% CI: [1.01, 2.57]) were more likely to be bullies. Adolescents who came from migrant families (OR = 1.37, 95% CI: [1.03, 1.82]), with low maternal education levels (OR = 1.42, 95% CI: [1.06, 1.91]) engaged in more negative bystander intervention behaviors. Furthermore, adolescents with less educated mothers experienced a higher fear of being bullied (never versus sometimes: OR = 1.33, 95% CI: [1.00, 1.85]; never versus usually OR = 1.39, 95% CI: [1.01, 1.20]).

Conclusions: A systematic examination of the relationship between school bullying and demographic household characteristics may be used to inform school policies on bullying, such as training management on the importance of paying attention to adolescents from disadvantage household backgrounds. Identifying demographic factors that may predict bullying can also be used to prevent individuals from becoming involved in bullying and reduce the related negative consequences from being bullied.
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http://dx.doi.org/10.1186/s12889-021-12367-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8665508PMC
December 2021

Excessive and less complex body movement in children with autism during face-to-face conversation: An objective approach to behavioral quantification.

Autism Res 2021 Nov 27. Epub 2021 Nov 27.

Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, China.

The majority of existing studies investigating characteristics of overt social behavior in individuals with autism spectrum disorder (ASD) relied on informants' evaluation through questionnaires and behavioral coding techniques. As a novelty, this study aimed to quantify the complex movements produced during social interactions in order to test differences in ASD movement dynamics and their convergence, or lack thereof, during social interactions. Twenty children with ASD and twenty-three children with typical development (TD) were videotaped while engaged in a face-to-face conversation with an interviewer. An image differencing technique was utilized to extract the movement time series. Spectral analyses were conducted to quantify the average power of movement, and the fractal scaling of movement. The degree of complexity matching was calculated to capture the level of behavioral coordination between the interviewer and children. Results demonstrated that the average power was significantly higher (p < 0.01), and the fractal scaling was steeper (p < 0.05) in children with ASD, suggesting excessive and less complex movement as compared to the TD peers. Complexity matching occurred between children and interviewers, but there was no reliable difference in the strength of matching between the ASD and TD children. Descriptive trends in the interviewer's behavior suggest that her movements adapted to match both ASD and TD movements equally well. The findings of our study might shed light on seeking novel behavioral markers of ASD, and on developing automatic ASD screening techniques during daily social interactions.
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http://dx.doi.org/10.1002/aur.2646DOI Listing
November 2021

Restricted Kinematics in Children With Autism in the Execution of Complex Oscillatory Arm Movements.

Front Hum Neurosci 2021 3;15:708969. Epub 2021 Nov 3.

Developmental Behavioral Pediatric Department, Shenzhen Baoan Women's and Children's Hospital, Jinan University, Shenzhen, China.

Restricted and repetitive behavior is a core symptom of autism spectrum disorder (ASD) characterized by features of restrictedness, repetition, rigidity, and invariance. Few studies have investigated how restrictedness is manifested in motor behavior. This study aimed to address this question by instructing participants to perform the utmost complex movement. Twenty children with ASD and 23 children with typical development (TD) performed one-dimensional, left-right arm oscillations by demonstrating varying amplitudes and frequencies. The entropy of amplitude and velocity was calculated as an index of kinematic complexity. Results showed that the velocity entropy, but not the amplitude entropy, was significantly lower in ASD than in TD ( < 0.01), suggesting restricted kinematics. Further analysis demonstrated that a significantly higher proportion of the velocity values was allocated at a low-speed level in the children with ASD ( < 0.01). A qualitative comparison of the complex movement with movement at preferred frequency suggested that the children with ASD might be less likely to shift away from the preferred movement. However, our study can be improved in terms of recruiting a larger sample of participants, measuring the level of motivation, and collecting both complex and preferred movements of the same participant.
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http://dx.doi.org/10.3389/fnhum.2021.708969DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8597710PMC
November 2021

Comprehensive Analysis of N6-Methyladenosine RNA Methylation Regulators Expression Identify Distinct Molecular Subtypes of Myocardial Infarction.

Front Cell Dev Biol 2021 27;9:756483. Epub 2021 Oct 27.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Myocardial infarction (MI) is one of the leading threats to human health. N6-methyladenosine (m6A) modification, as a pivotal regulator of messenger RNA stability, protein expression, and cellular processes, exhibits important roles in the development of cardiac remodeling and cardiomyocyte contractile function. The expression levels of m6A regulators were analyzed using the GSE5406 database. We analyzed genome-wide association study data and single-cell sequencing data to confirm the functional importance of m6A regulators in MI. Three molecular subtypes with different clinical characteristics were established to tailor treatment strategies for patients with MI. We applied pathway analysis and differentially expressed gene (DEG) analysis to study the changes in gene expression and identified four common DEGs. Furthermore, we constructed the protein-protein interaction network and confirmed several hub genes in three clusters of MI. To lucubrate the potential functions, we performed a ClueGO analysis of these hub networks. In this study, we identified that the levels of FTO, YTHDF3, ZC3H13, and WTAP were dramatically differently expressed in MI tissues compared with controls. Bioinformatics analysis showed that DEGs in MI were significantly related to modulating calcium signaling and chemokine signaling, and m6A regulators were related to regulating glucose measurement and elevated blood glucose levels. Furthermore, genome-wide association study data analysis showed that WTAP single-nucleotide polymorphism was significantly related to the progression of MI. In addition, single-cell sequencing found that WTAP is widely expressed in the heart tissues. Moreover, we conducted consensus clustering for MI in view of the dysregulated m6A regulators' expression in MI. According to the expression levels, we found MI patients could be clustered into three subtypes. Pathway analysis showed the DEGs among different clusters in MI were assigned to HIF-1, IL-17, MAPK, PI3K-Akt signaling pathways, etc. The module analysis detected several genes, including BAG2, BAG3, MMP2, etc. We also found that MI-related network was significantly related to positive and negative regulation of angiogenesis and response to heat. The hub networks in MI clusters were significantly related to antigen processing and ubiquitin-mediated proteolysis, RNA splicing, and stability, indicating that these processes may contribute to the development of MI. Collectively, our study could provide more information for understanding the roles of m6A in MI, which may provide a novel insight into identifying biomarkers for MI treatment and diagnosis.
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http://dx.doi.org/10.3389/fcell.2021.756483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8578940PMC
October 2021

Aberrant large-scale brain modules in deficit and non-deficit schizophrenia.

Prog Neuropsychopharmacol Biol Psychiatry 2022 03 21;113:110461. Epub 2021 Oct 21.

Department of Psychology, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address:

Objective: Schizophrenia is a heterogenous psychiatric disease, and deficit schizophrenia (DS) is a clinical subgroup with primary and enduring negative symptoms. Although previous neuroimaging studies have identified functional connectome alterations in schizophrenia, the modular organizations in DS and nondeficit schizophrenia (NDS) remain poorly understood. Therefore, this study aimed to investigate the modular-level alterations in DS patients compared with the NDS and healthy control (HC) groups.

Methods: A previously collected dataset was re-analyzed, in which 74 chronic male schizophrenia patients (33 DS and 41 NDS) and 40 HC underwent resting-state functional magnetic resonance imaging with eyes closed in a Siemens 3 T scanner (scanning duration = 8 min). Modular- (intramodule and intermodule connectivity) and nodal- [normalized within-module degree (Z) and participation coefficient (PC)] level graph theory properties were computed and compared among the three groups. Receiver operating characteristic curve (ROC) analyses were performed to examine the classification ability of these measures, and partial correlations were conducted between network measures and symptom severity. Validation analyses on head motion, network sparsity, and parcellation scheme were also performed.

Results: Both schizophrenia subgroups showed decreased intramodule connectivity in salience network (SN), somatosensory-motor network (SMN), and visual network (VN), and increased intermodule connectivity in SMN-default mode network (DMN) and SMN-frontoparietal network (FPN). Compared with NDS patients, DS patients showed weaker intramodule connectivity in SN and stronger intermodule connectivity in SMN-FPN and SMN-VN. At the nodal level, the schizophrenia-related alterations were distributed in SN, SMN, VN, and DMN, and 7 DS-specific nodal alterations were identified. Intramodule connectivity of SN, intermodule connectivity of SMN-VN, and Z of left precuneus successfully distinguished the three groups. Partial correlational analyses revealed that these measures were related to negative symptoms, general psychiatric symptoms, and neurocognitive function.

Conclusion: Our findings suggest that functional connectomes, especially SN, SMN, and VN, may capture the distinct and common disruptions of DS and NDS. These findings may help to understand the neuropathology of negative symptoms of schizophrenia and inform targets for treating different schizophrenia subtypes.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110461DOI Listing
March 2022

Correction to: Random and Short‑Term Excessive Eye Movement in Children with Autism During Face‑to‑Face Conversation.

J Autism Dev Disord 2021 Oct 5. Epub 2021 Oct 5.

Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, China.

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http://dx.doi.org/10.1007/s10803-021-05294-0DOI Listing
October 2021

Random and Short-Term Excessive Eye Movement in Children with Autism During Face-to-Face Conversation.

J Autism Dev Disord 2021 Aug 28. Epub 2021 Aug 28.

Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, China.

This study investigated the oculomotor performance in children with autism spectrum disorder (ASD) during a face-to-face conversation. A head mounted eye tracker recorded the eye movements in 20 children with ASD and 23 children with typical development (TD). Group comparisons were conducted on the randomness and the quantity of eye movement. The amount of time needed to reveal group difference was also examined. Results showed that the randomness of eye movement was significantly higher at all examined time durations, and the amount of eye movement was significantly greater within 3 s in the ASD group. These findings demonstrated an atypical pattern of oculomotor dynamics in children ASD, which might facilitate the objective identification of ASD during daily social interaction.
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http://dx.doi.org/10.1007/s10803-021-05255-7DOI Listing
August 2021

Classification of Children With Autism and Typical Development Using Eye-Tracking Data From Face-to-Face Conversations: Machine Learning Model Development and Performance Evaluation.

J Med Internet Res 2021 08 26;23(8):e29328. Epub 2021 Aug 26.

Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, China.

Background: Previous studies have shown promising results in identifying individuals with autism spectrum disorder (ASD) by applying machine learning (ML) to eye-tracking data collected while participants viewed varying images (ie, pictures, videos, and web pages). Although gaze behavior is known to differ between face-to-face interaction and image-viewing tasks, no study has investigated whether eye-tracking data from face-to-face conversations can also accurately identify individuals with ASD.

Objective: The objective of this study was to examine whether eye-tracking data from face-to-face conversations could classify children with ASD and typical development (TD). We further investigated whether combining features on visual fixation and length of conversation would achieve better classification performance.

Methods: Eye tracking was performed on children with ASD and TD while they were engaged in face-to-face conversations (including 4 conversational sessions) with an interviewer. By implementing forward feature selection, four ML classifiers were used to determine the maximum classification accuracy and the corresponding features: support vector machine (SVM), linear discriminant analysis, decision tree, and random forest.

Results: A maximum classification accuracy of 92.31% was achieved with the SVM classifier by combining features on both visual fixation and session length. The classification accuracy of combined features was higher than that obtained using visual fixation features (maximum classification accuracy 84.62%) or session length (maximum classification accuracy 84.62%) alone.

Conclusions: Eye-tracking data from face-to-face conversations could accurately classify children with ASD and TD, suggesting that ASD might be objectively screened in everyday social interactions. However, these results will need to be validated with a larger sample of individuals with ASD (varying in severity and balanced sex ratio) using data collected from different modalities (eg, eye tracking, kinematic, electroencephalogram, and neuroimaging). In addition, individuals with other clinical conditions (eg, developmental delay and attention deficit hyperactivity disorder) should be included in similar ML studies for detecting ASD.
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http://dx.doi.org/10.2196/29328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440949PMC
August 2021

Decreased neuregulin1β1 in first episode and drug-naïve patients with schizophrenia: Negative correlation with cognitive impairment.

Psychiatry Res 2021 10 5;304:114164. Epub 2021 Aug 5.

Medical College of Yangzhou University, Yangzhou 225003, PR China; Institute of Mental Health, Suzhou Psychiatric Hospital, The Affiliated Guangji Hospital of Soochow University, Suzhou, Jiangsu, 215137, PR China. Electronic address:

Background: Neuregulin1β1 (NRG1β1) is essential for neuronal migration during development and for the ongoing neural plasticity underlying cognitive function. This study investigated the relationship between cognitive impairment and serum NRG1β1 concentration in first-episode drug-naïve (FEDN) patients with schizophrenia.

Method: We measured serum NRG1β1 from 65 FEDN schizophrenia patients and 67 healthy matched controls. Cognitive function was evaluated using the Hopkins Vocabulary Learning Test-Revised (HVLT-R), Verbal Fluency Test (VFT), Trail Making Test (TMT), Digit Span Test (DST), and Stroop Test.

Results: Serum NRG1β1 concentration was significantly lower in the FEDN patient group than the control group (7.25±0.49 vs. 12.52±0.77 ng/mL; F=23.716, P<0.0001, Cohen's d=1.00). Further, serum NRG1β1 concentration in FEDN schizophrenia patients was negatively correlated with TMT-part A score (r=-0.408, P=0.001) and positively correlated with Stroop color subtest score (r=0.246, P=0.048). Multiple regression analysis also revealed weak correlations among FEDN patients between TMT-part A score and both serum NRG1β1 concentration (R=0.116, F=8.235, P=0.011) and duration of untreated psychosis (R=0.193, F=5.969, P=0.017).

Conclusion: This preliminary study suggests that serum NRG1β1 levels are reduced in FEDN patients with schizophrenia and that NRG1β1 may be involved in the cognitive function.
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http://dx.doi.org/10.1016/j.psychres.2021.114164DOI Listing
October 2021

E2F1 transcriptionally regulates CCNA2 expression to promote triple negative breast cancer tumorigenicity.

Cancer Biomark 2021 Jul 30. Epub 2021 Jul 30.

Department of Oncology, The First Hospital of Lanzhou University, Lanzhou, Gansu, China.

Background: Triple-negative breast cancer (TNBC) is a highly malignant breast cancer subtype with a poor prognosis. The cell cycle regulator cyclin A2 (CCNA2) plays a role in tumor development. Herein, we explored the role of CCNA2 in TNBC.

Methods: We analyzed CCNA2 expression in 15 pairs of TNBC and adjacent tissues and assessed the relationship between CCNA2 expression using the tissue microarray cohort. Furthermore, we used two TNBC cohort datasets to analyze the correlation between CCNA2 and E2F transcription factor 1 (E2F1) and a luciferase reporter to explore their association. Through rescue experiments, we analyzed the effects of E2F1 knockdown on CCNA2 expression and cellular behavior.

Results: We found that CCNA2 expression in TNBC was significantly higher than that in adjacent tissues with similar observations in MDA-MB-231 and MDA-MB-468 cells. E2F1 was highly correlated with CCNA2 as observed through bioinformatics analysis (R= 0.80, P< 0.001) and through TNBC tissue verification analysis (R= 0.53, P< 0.001). We determined that E2F1 binds the +677 position within the CCNA2 promoter. Moreover, CCNA2 overexpression increased cell proliferation, invasion, and migration owing to E2F1 upregulation in TNBC.

Conclusion: Our data indicate that E2F1 promotes TNBC proliferation and invasion by upregulating CCNA2 expression. E2F1 and CCNA2 are potential candidates that may be targeted for effective TNBC treatment.
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http://dx.doi.org/10.3233/CBM-210149DOI Listing
July 2021

Favored outgroup can eliminate the ingroup reference effect.

Scand J Psychol 2021 Dec 21;62(6):871-877. Epub 2021 Jul 21.

School of Psychology, Shaanxi Normal University, Xi'an, China.

Studies have found that, consistent with the ingroup favoritism effect, the recollection of ingroup related information is better than that of outgroup related information. This study explores whether preferred outgroup can eliminate the ingroup reference effect. Using the university as the reference group, Experiment 1 was designed to verify the group reference effect when there was no outgroup favoritism, and Experiment 2 examined the impact of outgroup favoritism on the group-reference effect. Experiment 1 found that recognition rates in the ingroup-reference condition were significantly higher than in the outgroup reference condition. Experiment 2 found no significant difference between recognition rates in the ingroup- reference and outgroup-reference conditions. Using household registration to classify the reference group, Experiment 3 recruited participants from rural areas to verify the group-reference effect while Experiment 4 recruited students from rural areas with a preference toward urban areas to explore the impact of outgroup favoritism on the group-reference effect. The results are the same as those in the Experiment 1 and Experiment 2. These findings suggest that the ingroup reference effect disappears when outgroups are preferred groups.
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http://dx.doi.org/10.1111/sjop.12767DOI Listing
December 2021

Identifying Autism with Head Movement Features by Implementing Machine Learning Algorithms.

J Autism Dev Disord 2021 Jul 11. Epub 2021 Jul 11.

Institute of Human Factors and Ergonomics, College of Mechatronics and Control Engineering, Shenzhen University, 3688 Nanhai, Avenue, Shenzhen City, Guangdong Province, China.

Our study investigated the feasibility of using head movement features to identify individuals with autism spectrum disorder (ASD). Children with ASD and typical development (TD) were required to answer ten yes-no questions, and they were encouraged to nod/shake head while doing so. The head rotation range (RR) and the amount of rotation per minute (ARPM) in the pitch (head nodding direction), yaw (head shaking direction) and roll (lateral head inclination) directions were computed, and further fed into machine learning classifiers as the input features. The maximum classification accuracy of 92.11% was achieved with the decision tree classifier with two features (i.e., RR_Pitch and ARPM_Yaw). Our study suggests that head movement dynamics contain objective biomarkers that could identify ASD.
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http://dx.doi.org/10.1007/s10803-021-05179-2DOI Listing
July 2021

The adsorption of cathinone drug on the platinum decorated silicon carbide nanosheets: DFT studies.

J Mol Model 2021 Jun 14;27(7):202. Epub 2021 Jun 14.

Independent Researcher, Suining, China.

Cathinone (CTN), classified as stimulants, is one of the psychoactive drugs. Although the sale of CTN was controlled by international drug laws, it was still sold on the internet, and its overdose caused many deaths worldwide. As chemical sensors, two-dimensional (2D) nanomaterials have drawn attention to be used in various biological molecules. In the current study, the sensing characteristics of the intrinsic SiC monolayer (SiCM) and its Pt-decorated state ([email protected]) were scrutinized toward the CTN drug by utilizing density functional theory (DFT) calculations. It was illustrated that the SiCM sensing response to CTN is insignificant (~ 10.6 at 298 K) due to the weak interaction with the adsorption energy of -0.25 eV. After the decoration of Pt on the SiCM, it was adsorbed above a hexagonal ring, which formed an η-Pt half-sandwich and the adsorption energy was -3.62 eV. It was found that the [email protected] strongly adsorbed CTN and the adsorption energy was -1.60 eV. Therefore, Pt-decoration augmented the SiCM sensing response to CTN from 10.3 to 716.6. The recovery time obtained for the CTN desorption from the [email protected] surface was 12.7 s, which was short. It was concluded that Pt-decoration makes the SiCM a favorable candidate for CTN identification.
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http://dx.doi.org/10.1007/s00894-021-04813-1DOI Listing
June 2021

Characteristics of Visual Fixation in Chinese Children with Autism During Face-to-Face Conversations.

J Autism Dev Disord 2021 Jun 8. Epub 2021 Jun 8.

Department of Child Psychiatry of Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, China.

Few eye tracking studies have examined how people with autism spectrum disorder (ASD) visually attend during live interpersonal interaction, and none with the Chinese population. This study used an eye tracker to record the gaze behavior in 20 Chinese children with ASD and 23 children with typical development (TD) when they were engaged in a structured conversation. Results demonstrated that children with ASD looked significantly less at the interlocutor's mouth and whole-face, and more at background. Additionally, gaze behavior was found to vary with the conversational topic. Given the great variability in eye tracking findings in existing literature, future explorations might consider investigating how fundamental factors (i.e., participant's characteristics, tasks, and context) influence the gaze behavior in people with ASD.
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http://dx.doi.org/10.1007/s10803-021-04985-yDOI Listing
June 2021

Facial expression stereotypes of rich and poor adults and children.

Cogn Process 2021 Nov 8;22(4):649-657. Epub 2021 Jun 8.

School of Psychology, Central China Normal University, Wuhan, China.

Facial expression stereotypes can affect the perception of other people's facial expressions. This study examined facial expression stereotypes of poor and rich adults and children. Experiment 1 found that the adult participants associated rich adults with positive emotions (i.e., happiness) and poor adults with negative emotions (i.e., sadness). In Experiments 2-4, adult participants still thought that rich 4-, 6-, and 10-year-old children would show positive facial expressions (happiness) but did not think that poor 4-, 6-, and 10-year-old children would show negative emotions (sadness). These finding have implications concerning how adults communicate with poor and rich adults and children.
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http://dx.doi.org/10.1007/s10339-021-01040-7DOI Listing
November 2021

Construction of a Nine-MicroRNA-Based Signature to Predict the Overall Survival of Esophageal Cancer Patients.

Front Genet 2021 19;12:670405. Epub 2021 May 19.

Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: Esophageal cancer (EC) is a common malignant tumor. MicroRNAs (miRNAs) play a key role in the occurrence and metastasis and are closely related to the prognosis of EC. Therefore, it will provide a powerful tool to predict the overall survival (OS) of EC patients based on miRNAs expression in EC tissues and blood samples.

Methods: Five independent databases, TCGA, GSE106817, GSE113486, GSE122497, and GSE112264, were used to construct nine-miRna signature and nomograms for prognosis. The bioinformatics analysis was used to predict the enrichment pathways of targets.

Results: A total of 132 overexpressed miRNAs and 23 suppressed miRNAs showed significant differential expression in both EC serum and tissue samples compared with normal samples. We also showed that nine miRNAs were related to the prognosis of EC. Higher levels of miR-15a-5p, miR-92a-3p, miR-92a-1-5p, miR-590-5p, miR-324-5p, miR-25-3p, miR-181b-5p, miR-421, and miR-93-5p were correlated to the shorter survival time in patients with EC. In addition, we constructed a risk prediction model based on the levels of nine differentially expressed miRNAs (DEMs) and found that the OS time of EC patients with high-risk score was shorter than that of EC patients with low-risk score. Furthermore, our results showed that the risk prediction scores of EC samples were higher than those of normal samples. Finally, the area under the curve (AUC) was used to analyze the risk characteristics of EC and normal controls. By calculating the AUC and the calibration curve, the RNA signature showed a good performance. Bioinformatics analysis showed that nine DEMs were associated with several crucial signaling, including p53, FoxO, PI3K-Akt, HIF-1, and TORC1 signaling. Finally, 14 messenger RNAs (mRNAs) were identified as hub targets of nine miRNAs, including BTRC, SIAH1, RNF138, CDC27, NEDD4L, MKRN1, RLIM, FBXO11, RNF34, MYLIP, FBXW7, RNF4, UBE3C, and RNF111. TCGA dataset validation showed that these hub targets were significantly differently expressed in EC tissues compared with normal samples.

Conclusion: We have constructed maps and nomograms of nine-miRna risk signals associated with EC prognosis. Bioinformatics analysis revealed that nine DEMs were associated with several crucial signaling, including p53, FoxO, PI3K-Akt, HIF-1, and TORC1 signaling, in EC. We think that this study will provide clinicians with an effective decision-making tool.
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http://dx.doi.org/10.3389/fgene.2021.670405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170160PMC
May 2021

Long non-coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR-9-3p in hepatocellular carcinoma.

Exp Ther Med 2021 Jul 12;22(1):755. Epub 2021 May 12.

Department of Hepatobiliary Surgery, People's Hospital of Dongying, Dongying, Shandong 257091, P.R. China.

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide with a low 5-year survival rate. Long non-coding RNA (lncRNA) double homeobox A pseudogene 8 (DUXAP8) is an oncogene and a potential biomarker in various tumors, such as ovarian, colorectal and non-small-cell lung cancer. However, the function and molecular mechanism underlying DUXAP8 in HCC progression is not completely understood. The expression of DUXAP8, microRNA (miR)-9-3p and insulin-like growth factor 1 receptor (IGF1R) in HCC tissues and cells was detected via reverse transcription-quantitative PCR. The expression levels of IGF1R and epithelial-mesenchymal transition-associated proteins (Snail, Slug, E-cadherin, N-cadherin and vimentin) were assessed via western blotting. The effects of DUXAP8, miR-9-3p and IGF1R on proliferation, migration and invasion were examined by conducting Cell Counting Kit-8 and Transwell assays, respectively. The interaction between miR-9-3p and DUXAP8 or IGF1R was predicted using StarBase or TargetScan, and further assessed using dual luciferase reporter and RNA immunoprecipitation assays. DUXAP8 and IGF1R were upregulated and miR-9-3p was downregulated in HCC tissues and cells compared with adjacent healthy tissues and a normal liver cell line, respectively. miR-9-3p overexpression decreased the protein expression level of IGF1R, and miR-9-3p knockdown enhanced the protein expression level of IGF1R in HCC cells compared with the corresponding control groups. Moreover, compared with the corresponding control groups, DUXAP8 knockdown and miR-9-3p overexpression increased E-cadherin protein expression levels, and decreased Snail, Slug, N-cadherin and vimentin protein expression levels. However, miR-9-3p inhibitor and IGF1R overexpression reversed DUXAP8 knockdown- and miR-9-3p overexpression-induced effects, respectively. In addition, compared with the corresponding control groups, DUXAP8 knockdown and miR-9-3p overexpression suppressed proliferation, migration and invasion, which was reversed by miR-9-3p inhibitor and IGF1R overexpression, respectively. Moreover, miR-9-3p as the target of DUXAP8 and IGF1R as the target of miR-9-3p were verified in HCC cells. lncRNA DUXAP8 contributed to HCC tumorigenesis via the miR-9-3p/IGF1R axis, providing a novel therapeutic approach for HCC diagnosis and treatment.
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http://dx.doi.org/10.3892/etm.2021.10187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8135127PMC
July 2021

Synthetic reactions driven by electron-donor-acceptor (EDA) complexes.

Beilstein J Org Chem 2021 6;17:771-799. Epub 2021 Apr 6.

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

The reversible, weak ground-state aggregate formed by dipole-dipole interactions between an electron donor and an electron acceptor is referred to as an electron-donor-acceptor (EDA) complex. Generally, upon light irradiation, the EDA complex turns into the excited state, causing an electron transfer to give radicals and to initiate subsequent reactions. Besides light as an external energy source, reactions involving the participation of EDA complexes are mild, obviating transition metal catalysts or photosensitizers in the majority of cases and are in line with the theme of green chemistry. This review discusses the synthetic reactions concerned with EDA complexes as well as the mechanisms that have been shown over the past five years.
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http://dx.doi.org/10.3762/bjoc.17.67DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042489PMC
April 2021

A population-based predictive model predicting candidate for primary tumor surgery in patients with metastatic esophageal cancer.

J Thorac Dis 2021 Feb;13(2):870-882

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: The survival benefit of primary tumor surgery for metastatic esophageal cancer (mEC) patients has been observed, but methods for discriminating which individual patients would benefit from surgery have been poorly defined. Herein, a predictive model was developed to test the hypothesis that only certain metastatic patients would gain a survival benefit from primary tumor surgery.

Methods: Clinical data for patients with mEC were extracted from the Surveillance, Epidemiology and End Results (SEER) database [2004-2016] and then divided into surgery and no-surgery groups according to whether surgery was performed on the primary tumor. Propensity-score-matching (PSM) was performed to balance the confounding factors. We hypothesized that the patients who had undergone surgery and lived longer than the median cancer-specific-survival (CSS) of the no-surgery group could benefit from surgery. We constructed a nomogram to predict surgery benefit potential based on multivariable logistic-regression analysis using preoperative factors. The predictive performance of the nomogram was evaluated by the area under the receiver operating characteristic (AUC) and calibration curves. The clinical application value of the nomogram was estimated with decision curve analysis (DCA).

Results: A total of 5,250 eligible patients with mEC were identified, and 9.4% [492] received primary tumor surgery. After PSM, CSS for the surgery group was significantly longer [median: 19 9 months; hazard ratio (HR) 0.52, P<0.001] compared with the no-surgery group. Among the surgery group, 69.3% [327] survived >9 months (surgery-beneficial group). The prediction nomogram showed good discrimination both in training and validation sets (AUC: 0.72 and 0.70, respectively), and the calibration curves indicated a good consistency. DCA demonstrated that the nomogram was clinically useful. According to this nomogram, surgery patients were classified into two groups: no-benefit-candidate and benefit-candidate. The benefit-candidate group was associated with longer survival than the no-benefit-candidate group (median CSS: 19 6.5 months, P<0.001). Additionally, there was no difference in survival between the no-benefit-candidate and no-surgery groups (median CSS: 6.5 9 months, P=0.070).

Conclusions: A predictive model was created for the selection of candidates for surgical treatment among mEC patients. This predictive model might be used to select patients who may benefit from primary tumor surgery.
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http://dx.doi.org/10.21037/jtd-20-2347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947545PMC
February 2021

CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer.

Cancer Manag Res 2021 9;13:1145-1158. Epub 2021 Feb 9.

Department of Pathology, Peking University Cancer Hospital & Institute, Beijing City, People's Republic of China.

Background: Breast cancer (BC) is a highly heterogeneous malignant tumor that affects women's health. Circular RNAs (circRNAs) are involved in tumor growth in many cancers. However, the role of hsa_circ_0101187 (circYY1) in BC is still unclear.

Methods: Expression of circYY1, microRNA (miR)-769-3p, and YY1 (Yin Yang 1) mRNA was tested by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, colony formation, migration, and invasion were analyzed with MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), colony formation, and transwell assays. Glucose uptake, lactate product, and ATP (adenosine triphosphate) content were detected with corresponding kits. Several protein levels were measured with Western blotting. The regulatory mechanisms of the circYY1, miR-769-3p, and YY1 were validated by RNA immunoprecipitation (RIP) assay, dual-luciferase reporter assay, and/or RNA pull-down assay. The role of circYY1 in BC was confirmed by xenograft assay.

Results: CircYY1 and YY1 were upregulated in BC, while miR-769-3p had an opposing result. Also, BC patients with high circYY1 expression had a poor prognosis. Downregulation of circYY1 decreased xenograft tumor growth in vivo. Both circYY1 inhibition and miR-769-3p elevation constrained BC cell viability, colony formation, migration, invasion, and glycolysis in vitro. CircYY1 acted as a sponge for miR-769-3p, which targeted YY1. CircYY1 sponged miR-769-3p to modulate YY1 expression. Both miR-769-3p inhibition and YY1 upregulation antagonized circYY1 silencing-mediated influence on malignancy and glycolysis of BC cells.

Conclusion: CircYY1 promoted glycolysis and tumor growth via increasing YY1 expression through sponging miR-769-3p in BC, offering a promising therapeutic target and prognostic biomarker for BC.
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http://dx.doi.org/10.2147/CMAR.S289172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881944PMC
February 2021

Measuring Public Reaction to Violence Against Doctors in China: Interrupted Time Series Analysis of Media Reports.

J Med Internet Res 2021 02 16;23(2):e19651. Epub 2021 Feb 16.

College of Computer Science and Technology, Zhejiang University, Hangzhou, China.

Background: Violence against doctors in China is a serious problem that has attracted attention from both domestic and international media.

Objective: This study investigates readers' responses to media reports on violence against doctors to identify attitudes toward perpetrators and physicians and examine if such trends are influenced by national policies.

Methods: We searched 17 Chinese violence against doctors reports in international media sources from 2011 to 2020. We then tracked back the original reports and web crawled the 19,220 comments in China. To ascertain the possible turning point of public opinion, we searched violence against doctors-related policies from Tsinghua University ipolicy database from 2011 to 2020, and found 19 policies enacted by the Chinese central government aimed at alleviating the intense patient-physician relationship. We then conducted a series of interrupted time series analyses to examine the influence of these policies on public sentiment toward violence against doctors over time.

Results: The interrupted time series analysis (ITSA) showed that the change in public sentiment toward violence against doctors reports was temporally associated with government interventions. The declarations of 10 of the public policies were followed by increases in the proportion of online public opinion in support of doctors (average slope changes of 0.010, P<.05). A decline in the proportion of online public opinion that blamed doctors (average level change of -0.784, P<.05) followed the declaration of 3 policies.

Conclusions: The government's administrative interventions effectively shaped public opinion but only temporarily. Continued public policy interventions are needed to sustain the reduction of hostility toward medical doctors.
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http://dx.doi.org/10.2196/19651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925148PMC
February 2021

The group-reference effect and soldiers' recognition memory.

Memory 2021 02 11;29(2):146-152. Epub 2021 Feb 11.

School of Psychology, Shaanxi Normal University, Xian, People's Republic of China.

Previous research has consistently found that the self-reference effect (SRE) is equal to, or stronger than, the group-reference effect (GRE) for memory performance. The military strongly emphasises group identity; this study investigated whether the GRE was stronger than the SRE for soldiers. Soldiers were recruited to participate in Experiments 1 and Experiment 2. Experiment 1 revealed that recognition was better under the group-reference condition than the self-reference condition. Experiment 2 was identical to Experiment 1, with the exception that the recognition test required participants to use "remember" / "know" / "guess" judgments. The results were consistent with those of Experiment 1, that is, the GRE contributed to better recognition than the SRE, but the difference was statistically significant only for "know" responses. Using a less cohesive group (university students) as participants, Experiment 3 found that the GRE was not superior to the SRE for memory recognition, which indicated that the results of Experiments 1 and 2 were exclusive to soldiers. The findings suggest that soldiers' sense of self might be unique, and that an ingroup sense of self might be dominant for soldiers.
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http://dx.doi.org/10.1080/09658211.2020.1866014DOI Listing
February 2021

Atypical Head Movement during Face-to-Face Interaction in Children with Autism Spectrum Disorder.

Autism Res 2021 06 2;14(6):1197-1208. Epub 2021 Feb 2.

Institute of Human Factors and Ergonomics, College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen, China.

The present study implemented an objective head pose tracking technique-OpenFace 2.0 to quantify the three dimensional head movement. Children with autism spectrum disorder (ASD) and typical development (TD) were engaged in a structured conversation with an interlocutress while wearing an eye tracker. We computed the head movement stereotypy with multiscale entropy analysis. In addition, the head rotation range (RR) and the amount of rotation per minute (ARPM) were calculated to quantify the extent of head movement. Results demonstrated that the ASD group had significantly higher level of movement stereotypy, RR and ARPM in all the three directions of head movement. Further analyses revealed that the extent of head movement could be significantly explained by movement stereotypy, but not by the amount of visual fixation to the interlocutress. These results demonstrated the atypical head movement dynamics in children with ASD during live interaction. It is proposed that head movement might potentially provide novel objective biomarkers of ASD. LAY SUMMARY: Our study used an objective tool to quantify head movement in children with autism. Results showed that children with autism had more stereotyped and greater head movement. We suggest that head movement tracking technique be widely used in autism research.
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http://dx.doi.org/10.1002/aur.2478DOI Listing
June 2021

The value of enhanced CT scanning for predicting lymph node metastasis along the right recurrent laryngeal nerve in esophageal squamous cell carcinoma.

Ann Transl Med 2020 Dec;8(24):1632

Department of Thoracic Surgery, Section of Esophageal Surgery, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

Background: The right recurrent laryngeal nerve (RRLN) is the region most prone to lymph node metastasis in esophageal squamous cell carcinoma (ESCC). Nodal involvement may be underestimated by traditional imaging prediction criteria, such as a short axis diameter of 10 mm. The purpose of this study was to determine a more accurate imaging criterion to guide clinical treatment strategy selection.

Methods: The clinical data of 307 patients with thoracic ESCC who underwent surgery at Shanghai Chest Hospital between January 2018 and December 2018 were retrospectively analyzed. Utilizing 1-mm layer thickness enhanced computed tomography (CT), the RRLN lymph node short diameter (LNSD) size was measured. Univariate and multivariate analyses were performed to determine the risk factors for lymph node metastasis along the RRLN.

Results: In our study, RRLN lymph node metastasis occurred in 60 (19.5%) patients and general lymph node metastasis occurred in 150 (48.9%) patients. Of the resected lymph nodes along the RRLN, 14.5% (121/832) were positive. Multivariate analysis identified LNSD [odds ratio (OR), 1.236] as an independent risk factor for RRLN lymph node metastasis. In CT evaluation, a short diameter of 6.5 mm in the RRLN lymph nodes is a critical predictor of metastasis at this site (sensitivity =50%, specificity =83.4%) and a larger short diameter was associated with a higher risk of metastasis (P<0.001).

Conclusions: A 6.5 mm cutoff in LNSD can be applied to clinically predict lymph node metastasis in the RRLN region for patients with ESCC.
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http://dx.doi.org/10.21037/atm-20-4991DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812183PMC
December 2020

Clinical efficacy of bypass grafting in recurrent nasopharyngeal carcinoma patients with internal carotid artery invasion.

Am J Otolaryngol 2021 May-Jun;42(3):102860. Epub 2021 Jan 12.

Department of Neurosurgery, Third Affiliated Hospital of Southern Medical University, No.183 Zhongshan Avenue West, Tianhe District, Guangzhou 510665, China.

Background: We aimed to assess the clinical efficacy of bypass grafting in recurrent nasopharyngeal carcinoma patients with internal carotid artery invasion.

Methods: A retrospective analysis was performed involving 51 patients either operated by bypass grafting (n = 22) or treated with repeated chemo-radiotherapy (n = 29).

Results: Four patients in the bypass grafting group died 3-5 months after the operation due to epistaxis and pulmonary infection (4/22, 18.2%), and three more patients exhibited a modified Rankin Scale (mRS) ≥ 2 during the follow-up (3/22, 13.6%). In the repeated chemo-radiotherapy group, 8 patients died (8/29, 27.6%), including seven patients dying within 2-3 months due to epistaxis and pulmonary infection (7/29, 24.1%). One more patient died of epistaxis after 9 months. The difference in mortality between the two treatment groups within 3 months of treatment was statistically significant (P = 0.038).

Conclusions: Cerebrovascular reconstruction after detailed collateral flow assessment is an effective treatment for recurrent NPC patients with internal carotid artery invasion.
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http://dx.doi.org/10.1016/j.amjoto.2020.102860DOI Listing
October 2021

Effect of mannose-binding lectin gene polymorphisms on the risk of rheumatoid arthritis: Evidence from a meta-analysis.

Int J Rheum Dis 2021 Mar 17;24(3):300-313. Epub 2021 Jan 17.

Department of Clinical Medicine, Jiangxi Medical College, Shangrao, China.

Background: The effect of mannose-binding lectin (MBL) gene polymorphisms on susceptibility of rheumatoid arthritis (RA) were evaluated in ethnically different populations, whereas the results were always inconsistent.

Materials And Methods: Fourteen articles involving 36 datasets were recruited to evaluate the association between MBL gene polymorphisms and rheumatoid arthritis in a meta-analysis. The random or fixed effect models were used to evaluate the pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs).

Results: Stratified analysis by ethnicities was conducted and the result revealed that rs1800450 (T vs C, OR = 1.32, 95% CI: 1.04-1.67, P < .05) and MBL-A/O (T vs C, OR = 1.20, 95% CI: 1.08-1.34, P < .001) were strongly associated with RA in Brazilian populations. In addition, the significant relationship between rs11003125 (T vs C, OR = 1.16, 95% CI: 1.06-1.26, P < .05) with RA were also observed in East Asian populations. Meanwhile, the inverse associations between rs5030737 with RA in East Asians and rs1800450 with RA in Indians were acquired. However, no association between any MBL polymorphism with RA susceptibility was confirmed in Caucasians.

Conclusions: The structural polymorphisms in exon 1 of MBL gene may significantly contribute to susceptibility and development of RA in Brazilian and Indian populations, whereas the functional polymorphisms in the promoter region were more likely to associate with RA in East Asians.
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http://dx.doi.org/10.1111/1756-185X.14060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986746PMC
March 2021
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