Publications by authors named "XiaoLan Liu"

76 Publications

Magnetic [email protected]@β-cyclodextrin for solid phase extraction of methyl parathion and fenthion in lettuce samples.

Anal Methods 2021 Jun 11. Epub 2021 Jun 11.

Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.

In this study, magnetic [email protected]@β-cyclodextrin copolymerized microparticles were synthesized and applied for the extraction of methyl parathion and fenthion in lettuce samples followed by HPLC-UV detection. The magnetic β-cyclodextrin copolymerized microparticles were prepared by dispersion polymerization with acryloyl β-cyclodextrin as the functional monomer and ethylene glycol dimethyacrylate as the crosslinker. The composite magnetic microparticles were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, magnetic measurement, and thermogravimetric analysis, and used as the adsorbent of magnetic solid-phase extraction (MSPE) for methyl parathion and fenthion. The extraction conditions including sample pH and ionic strength, desorption solvent type and volume, and adsorption and desorption times were optimized. Under the optimal extraction conditions, an MSPE-HPLC-UV method was developed for the detection of methyl parathion and fenthion in lettuce. Wide linear ranges of 1.0-200 μg kg-1 (R2 = 0.9998) for methyl parathion and 1.5-200 μg kg-1 (R2 = 0.9978) for fenthion were obtained and the limits of detection were 0.3 μg kg-1 for methyl parathion and 0.5 μg kg-1 for fenthion in lettuce, respectively. The proposed method was applied for the determination of methyl parathion and fenthion in lettuce with satisfactory recoveries between 89.2-101.2%, and relative standard deviations were less than 9.1%. Thus, the MSPE-HPLC-UV method has high accuracy and sensitivity for the analysis of methyl parathion and fenthion in lettuce samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1ay00681aDOI Listing
June 2021

Distinct protocerebral neuropils associated with attractive and aversive female-produced odorants in the male moth brain.

Elife 2021 May 14;10. Epub 2021 May 14.

Chemosensory lab, Department of Psychology, Norwegian University of Science and Technology, Trondheim, Norway.

The pheromone system of heliothine moths is an optimal model for studying principles underlying higher-order olfactory processing. In , three male-specific glomeruli receive input about three female-produced signals, the primary pheromone component, serving as an attractant, and two minor constituents, serving a dual function, that is, attraction versus inhibition of attraction. From the antennal-lobe glomeruli, the information is conveyed to higher olfactory centers, including the lateral protocerebrum, via three main paths - of which the medial tract is the most prominent. In this study, we traced physiologically identified medial-tract projection neurons from each of the three male-specific glomeruli with the aim of mapping their terminal branches in the lateral protocerebrum. Our data suggest that the neurons' widespread projections are organized according to behavioral significance, including a spatial separation of signals representing attraction versus inhibition - however, with a unique capacity of switching behavioral consequence based on the amount of the minor components.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.65683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154038PMC
May 2021

ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation.

Nat Commun 2021 04 20;12(1):2346. Epub 2021 Apr 20.

Department of Biochemistry and Research Center of Clinical Pharmacy of The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Cancer expression of PD-L1 suppresses anti-tumor immunity. PD-L1 has emerged as a remarkable therapeutic target. However, the regulation of PD-L1 degradation is not understood. Here, we identify several compounds as inducers of PD-L1 degradation using a high-throughput drug screen. We find EGFR inhibitors promote PD-L1 ubiquitination and proteasomal degradation following GSK3α-mediated phosphorylation of Ser279/Ser283. We identify ARIH1 as the E3 ubiquitin ligase responsible for targeting PD-L1 to degradation. Overexpression of ARIH1 suppresses tumor growth and promotes cytotoxic T cell activation in wild-type, but not in immunocompromised mice, highlighting the role of ARIH1 in anti-tumor immunity. Moreover, combining EGFR inhibitor ES-072 with anti-CTLA4 immunotherapy results in an additive effect on both tumor growth and cytotoxic T cell activation. Our results delineate a mechanism of PD-L1 degradation and cancer escape from immunity via EGFR-GSK3α-ARIH1 signaling and suggest GSK3α and ARIH1 might be potential drug targets to boost anti-tumor immunity and enhance immunotherapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-22467-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058344PMC
April 2021

Moisture-sensitive metal-organic framework constructed from cobalt and 4-(4-pyridyl) benzoic acid for dispersive solid-phase extraction of polycyclic aromatic hydrocarbons in apple.

J Sep Sci 2021 Apr 14;44(8):1706-1715. Epub 2021 Mar 14.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, P. R. China.

A moisture-sensitive metal-organic framework Co (pybz) ·2DMF was synthesized and applied as the adsorbent of dispersive solid-phase extraction. The structure changed after water treatment due to the fact that two chelate carboxylate groups on the skeleton were transformed to monodentate because of the coordination of water molecules. The material showed good adsorption for fluorene, phenanthrene, fluoranthene, and pyrene in water because of the π-π conjugation and π-complexation effects. Coupled with high-performance liquid chromatography, a dispersive solid-phase extraction method of determining the content of fluorene, phenanthrene, fluoranthene, and pyrene in apple samples was established after optimizing the extraction conditions. Methanol containing 4% acetic acid was used as the effective eluent. The linearities were 0.5-1000 μg/kg for fluorene, phenanthrene and 5-1000 μg/kg for fluoranthene, pyrene. The limits of detection were 0.06-0.6 μg/kg, and the recoveries were 94.4-116.4%. The method has a high sensitivity for the determination of four polycyclic aromatic hydrocarbons in apple samples.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.202001158DOI Listing
April 2021

The complete mitochondrial genome and phylogenetic analysis of sugarcane ( spp. hybrids) line 15a-53.

Mitochondrial DNA B Resour 2020 Sep 21;5(3):3389-3391. Epub 2020 Sep 21.

Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan, PR China.

The complete mitogenome of spp. hybrid 15a-53 was determined in this study, which contains two distinct circular chromosomes, Chromosome 1 and 2. The length of Chromosome 1 is 300,848 bp with the GC content of 43.93%, while Chromosome 2 is 144,713 bp in length with the GC content of 43.57%. In Chromosome 1, 7.14% of the genome (21,468 nucleotides) is coding DNA and 92.86% (279,380 nucleotides) are intergenic region, while in Chromosome 2, 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% (132,848 nucleotides) are intergenic region. Chromosome 1 contains 20 protein-coding genes (three genes, three genes, two genes, one gene, one gene, six genes, and four genes), and 21 non-coding genes (15 tRNA and six rRNAs), while in Chromosome 2, there are 13 protein-coding genes (four genes, three genes, two genes, one gene, one gene, one gene, and one gene) and five tRNA genes. Maximum Likelihood phylogenetic analysis indicated that 15a-53 is close to spp. hybrid ROC22, spp. hybrid FN15 and Khon Kaen 3. The complete mitochondrial genome herein will provide useful sequence information for phylogenetic and evolutionary studies for Saccharum and Poaceae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1820395DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781999PMC
September 2020

The complete mitochondrial genome of sugarcane ( spp.) variety FN15.

Mitochondrial DNA B Resour 2020 27;5(3):2163-2165. Epub 2020 May 27.

Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan, China.

The complete mitogenome of spp. hybrid FN15 was successfully sequenced. It contains two distinct circular chromosomes, Chromosome 1 and Chromosome 2. The former is 301,533 bp in length with the GC content of 43.90%, and 7.12% of genome (21,468 nucleotides) are coding DNA while 92.88% of genome (280,065 nucleotides) are intergenic region. The latter is 144,744 bp in length with the GC content of 43.57%, and 8.20% of genome (11,865 nucleotides) are coding DNA and 91.80% of genome (132,879 nucleotides) are intergenic region. Besides, Chromosome 1 contains 22 protein-coding genes (four genes, three genes, three genes, one gene, one gene, six genes and four genes), and 21 non-coding genes (15 tRNA and six rRNAs), whereas in Chromosome 2, there are 13 protein-coding genes (two genes, one gene, one gene, one gene, one gene, four genes and three genes) and five tRNA genes. Maximum Likelihood phylogenetic analysis demonstrated that FN15 is close with spp. hybrid ROC22, Khon Kaen 3 and species. This complete mitochondrial genome will provide essential DNA molecular data for further phylogenetic and evolutionary analysis for .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2020.1768926DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510754PMC
May 2020

Autologous adipose mesenchymal stem cell administration in arteriosclerosis and potential for anti-aging application: a retrospective cohort study.

Stem Cell Res Ther 2020 12 11;11(1):538. Epub 2020 Dec 11.

Regenerative Medicine, Sun Field Clinic, TIME24 Building 1F 2-4-32 Aomi, Koto-ku, Tokyo, 135-0064, Japan.

Objective: Arteriosclerosis is an age-related disease and a leading cause of cardiovascular disease. In animal experiments, mesenchymal stem cells and its culture-conditioned medium have been shown to be promising tools for prevention or treatment of arteriosclerosis. On the basis of these evidences, we aimed to assess whether administration of autologous adipose-derived mesenchymal stem cells (Ad-MSC) is safe and effective for treatment of arteriosclerosis.

Methods: We retrospectively reviewed clinical records of patients with arteriosclerosis who had received autologous Ad-MSC administration at our clinic. Patients' characteristics were recorded and data on lipid profile, intimal-media thickness (IMT), cardio-ankle vascular index (CAVI), and ankle-brachial index (ABI) before and after Ad-MSC administration were collected and compared.

Results: Treatment with Ad-MSC significantly improved HDL, LDL, and remnant-like particle (RLP) cholesterol levels. No adverse effect or toxicity was observed in relation to the treatment. Of the patients with abnormal HDL values before treatment, the vast majority showed improvement in the values. Overall, the measurements after treatment were significantly increased compared with those before treatment (p < 0.01). In addition, decreases in LDL cholesterol and RLP levels were observed after treatment in patients who had abnormal LDL cholesterol or RLP levels before treatment. The majority of patients with pre-treatment abnormal CAVI values had improved values after treatment. In patients with available IMT values, a significant decrease in the IMT values was found after therapy (p < 0.01). All patients with borderline arteriosclerosis disease had improved laboratory findings after treatment. In general, post-treatment values were significantly decreased as compared with pre-treatment values. Of the patients with normal ABI values before treatment at the same time as CAVI, the vast majority remained normal after treatment.

Conclusions: These findings suggest that Ad-MSC administration is safe and effective in patients developing arteriosclerosis, thereby providing an attractive tool for anti-aging application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-020-02067-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733281PMC
December 2020

A novel serine protease with anticoagulant and fibrinolytic activities from the fruiting bodies of mushroom Agrocybe aegerita.

Int J Biol Macromol 2021 Jan 20;168:631-639. Epub 2020 Nov 20.

College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China; Heilongjiang Provincial Key Laboratory of Corn Deep Processing Theory and Techology, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, PR China. Electronic address:

A novel fibrinolytic enzyme, ACase was isolated from fruiting bodies of a mushroom, Agrocybe aegerita. ACase was purified by using ammonium sulfate precipitation, gel filtration, ion exchange and hydrophobic chromatographies to 237.12 fold with a specific activity of 1716.77 U/mg. ACase was found to be a heterodimer with molecular mass of 31.4 and 21.2 kDa by SDS-PAGE and appeared as a single band on Native-PAGE and fibrin-zymogram. The N-terminal sequence of the two subunits of ACase was AIVTQTNAPWGL (subunit 1) and SNADGNGHGTHV (subunit 2). ACase had maximal activity at 47 °C and pH 7.6. It's activity was improved by Cu, Na, Fe, Zn, Ba, K and Mn, but inhibited by Fe, Mg and Ca. PMSF, SBTI, aprotinine and Lys inhibited the enzyme activity, which suggested that ACase was a serine protease. ACase could degrade all three chains (α, β and γ) of fibrinogen. Moreover, the enzyme acted as both, a plasmin-like fibrinolytic enzyme and a plasminogen activator. It could hydrolyze human thrombin slightly, which indicated that the ACase could inhibit the activity of thrombin and acted as an anticoagulant to prevent thrombosis. Based on these results, ACase might act as a therapeutic agent for treating thrombosis, or as a functional food. Further investigation of the enzyme is underway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.11.118DOI Listing
January 2021

A robust all-inorganic hybrid energy harvester for synergistic energy collection from sunlight and raindrops.

Nanotechnology 2021 Feb;32(7):075401

Key Lab for Special Functional Materials of Ministry of Education, National & Local Joint Engineering Research Center for High-efficiency Display and Lighting Technology, Collaborative Innovation Center of Nano Functional Materials and Applications, School of Materials Science and Engineering, Henan University, Kaifeng 475004, People's Republic of China. School of Information Technology, Shangqiu Normal University, Shangqiu 476000, People's Republic of China.

As a new concept of the device, a hybrid energy harvester integrated with a water droplet triboelectric nanogenerator (WD-TENG) and a solar cell has been reported to convert raindrop energy and solar energy into electricity. However, organic triboelectric layers are usually utilized in previous studies that might be decomposed under long-term UV irradiation, resulting in degradation of the hybrid energy harvester. In this work, a fully inorganic hybrid energy harvester is demonstrated. Superhydrophobic SiO film is introduced to the system as both the triboelectric layer of the WD-TENG and the anti-reflective layer of the solar cell, which could increase the power conversion efficiency (PCE) of the solar cell from 15.17% to 15.71%. Meanwhile, WD-TENG with the SiO triboelectric layer could collect energies from rain droplets. This superhydrophobic SiO film could effectively reduce the dependence of the tilt angle for the WD-TENG and bring up self-cleaning performance for the hybrid energy harvester. Moreover, this fully inorganic architecture could enhance the stability of the hybrid energy harvester, making it a promising strategy in practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1088/1361-6528/abb84bDOI Listing
February 2021

Wheat Germ-Derived Peptides Exert Antiadhesive Activity against : Insights into Structural Characteristics of Identified Peptides.

J Agric Food Chem 2020 Oct 6;68(43):11954-11974. Epub 2020 Oct 6.

College of Food and Biological Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China.

Approximately 50-80% of the world population are infected with , which is categorized as a class I carcinogen. Antiadhesive therapy is emerging as a promising alternative to antibiotics against bacterial infection. This study demonstrated that defatted wheat germ protein hydrolysates (DWGPH) effectively inhibited adhesion to gastric epithelial cells. DWGPH prepared by pronase possessed the best activity where its inhibitory percentage at 10 mg/mL was 51.7 ± 6.8% and the minimum antiadhesive concentration was 0.31 mg/mL. The antiadhesive activity is attributable to peptides acting as receptor analogs in binding to . Peptides with potential -binding ability ( = 267) were identified, and their structural characteristics were comprehensively analyzed, including net charge, Boman index, instability index, aliphatic index, molecular weight, isoelectric point, hydrophobicity, and Hmoment (α-helix and β-sheet). This work provided an array of peptide sequences for further exploration as putative ligands of adhesins and for elucidating molecular mechanisms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.0c04367DOI Listing
October 2020

Heterosis-associated genes confer high yield in super hybrid rice.

Theor Appl Genet 2020 Dec 27;133(12):3287-3297. Epub 2020 Aug 27.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.

Key Message: Heterosis QTLs, including qSS7 and qHD8, with dominance effects were identified through GBS and large-scale phenotyping of CSSLs and hybrid F populations in a paddy field. Heterosis has contributed immensely to agricultural production, but its genetic basis is unclear. We evaluated dominance effects by creating two hybrid populations: a B-homo set with a homozygous background and heterozygous chromosomal segments and a B-heter set with a heterozygous background and homozygous segments. This was achieved by crossing a set of 156 backcrossed-derived chromosome segment substitution lines (CSSLs) with their recurrent parent (9311), the male parent of the first super-high-yield hybrid Liangyoupei9 (LYP9), and with the female parent (PA64s) of the hybrid. The CSSLs were subjected to a genotyping-by-sequencing analysis to develop a genetic map of segments introduced from the PA64s. We evaluated the heterotic effects on eight yield-related traits in the hybrid variety and F populations in large-scale field experiments over 2 years. Using a linkage map consisting of high-density SNPs, we identified heterosis-associated genes in LYP9. Five candidate genes contributed to the high yield of LYP9, with qSS7 and qHD8 repeatedly detected in both B-hybrid populations. The heterozygous segments harboring qSS7 and qHD8 showed dominance effects that contributed to the heterosis of yield components in the hybrid rice variety Liangyoupei9.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00122-020-03669-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7567734PMC
December 2020

Enhanced OsNLP4-OsNiR cascade confers nitrogen use efficiency by promoting tiller number in rice.

Plant Biotechnol J 2021 01 10;19(1):167-176. Epub 2020 Aug 10.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.

Increased use of nitrogen fertilizers has deleterious impact on the environment. Increase in yield potential at low nitrogen supply is regarded as a cereal breeding goal for future agricultural sustainability. Although natural variations of nitrogen transporters have been investigated, key genes associated with assimilation remain largely unexplored for nitrogen use efficiency (NUE) enhancement. Here, we identified a NIN-like protein NLP4 associated with NUE through a GWAS in rice. We found that OsNLP4 transactivated OsNiR encoding nitrite reductase that was critical in nitrogen assimilation in rice. We further constructed quadrupling NREs (Nitrate-responsive cis-elements) in the promoter of OsNiR (p4xNRE:OsNiR) and enhanced nitrogen assimilation significantly. We demonstrated that OsNLP4-OsNiR increased tiller number and yield through enhancing nitrogen assimilation and NUE. Our discovery highlights the genetic modulation of OsNLP4-OsNiR signalling cascade as a strategy for high NUE and yield breeding in rice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/pbi.13450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769241PMC
January 2021

Ca-Dependent NOX5 (NADPH Oxidase 5) Exaggerates Cardiac Hypertrophy Through Reactive Oxygen Species Production.

Hypertension 2020 09 20;76(3):827-838. Epub 2020 Jul 20.

From the Department of Cardiology, Renmin Hospital of Wuhan University, China (G.-J.Z., C.-L.Z., L.-H.Z., C.Z., X.-Y.Z., S.T., X.-J.Z., Z.-G.S., H.L.).

NOX5 (NADPH oxidase 5) is a homolog of the gp91 subunit of the phagocyte NOX, which generates reactive oxygen species. NOX5 is involved in sperm motility and vascular contraction and has been implicated in diabetic nephropathy, atherosclerosis, and stroke. The function of NOX5 in the cardiac hypertrophy is unknown. Because NOX5 is a Ca-sensitive, procontractile NOX isoform, we questioned whether it plays a role in cardiac hypertrophy. Studies were performed in (1) cardiac tissue from patients undergoing heart transplant for cardiomyopathy and heart failure, (2) NOX5-expressing rat cardiomyocytes, and (3) mice expressing human NOX5 in a cardiomyocyte-specific manner. Cardiac hypertrophy was induced in mice by transverse aorta coarctation and Ang II (angiotensin II) infusion. NOX5 expression was increased in human failing hearts. Rat cardiomyocytes infected with adenoviral vector encoding human NOX5 cDNA exhibited elevated reactive oxygen species levels with significant enlargement and associated increased expression of ANP (atrial natriuretic peptides) and β-MHC (β-myosin heavy chain) and prohypertrophic genes (, , and ) under Ang II stimulation. These effects were reduced by N-acetylcysteine and diltiazem. Pressure overload and Ang II infusion induced left ventricular hypertrophy, interstitial fibrosis, and contractile dysfunction, responses that were exaggerated in cardiac-specific trangenic mice. These phenomena were associated with increased reactive oxygen species levels and activation of redox-sensitive MAPK (mitogen-activated protein kinase). N-acetylcysteine treatment reduced cardiac oxidative stress and attenuated cardiac hypertrophy in trangenic. Our study defines Ca-regulated NOX5 as an important NOX isoform involved in oxidative stress- and MAPK-mediated cardiac hypertrophy and contractile dysfunction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1161/HYPERTENSIONAHA.120.15558DOI Listing
September 2020

A hollow microshuttle-shaped capsule covalent organic framework for protein adsorption.

J Mater Chem B 2019 03 11;7(9):1469-1474. Epub 2019 Feb 11.

Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan 650091, P. R. China.

Covalent organic frameworks (COFs) are a class of crystalline porous materials utilized in various potential applications. However, the development of hollow-structured COFs with defined morphology is important for their further applications, which is rare. Herein, COF with unique hollow shuttle morphology was prepared by a Schiff condensation reaction between 4-(4-aldehyde phenyl)ethylene (TPE) and tetra-(4-aminophenyl) porphyrin (TAP). A detailed mechanistic investigation reveals that an initial self-assembly followed by a similar inside-out Ostwald ripening process is responsible for the hollow capsule formation. The hollow microshuttle-shaped capsule COF is used for studying hemoglobin adsorption, which shows an uptake of 550.82 mg g of hemoglobin. These studies could foreshadow new avenues for the development of porous materials with defined morphologies for the adsorption of biomolecules.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c8tb02870bDOI Listing
March 2019

Dimethyl sulfoxide, a potent oral radioprotective agent, confers radioprotection of hematopoietic stem and progenitor cells independent of apoptosis.

Free Radic Biol Med 2020 06 25;153:1-11. Epub 2020 Mar 25.

Department of Pathophysiology, Beijing Institute of Radiation Medicine, Beijing Key Laboratory for Radiobiology (BKLRB), Beijing, PR China. Electronic address:

In mass casualty events involving radiation exposure, there is a substantial unmet need for identifying and developing an orally bioavailable agent that can be used to protect the hematopoietic stem cell pool and regenerate hematopoiesis after radiation injury. Dimethyl sulfoxide (DMSO), a free-radical scavenger, has shown therapeutic benefits in many preclinical and clinical studies. This study investigates the radioprotective effects of DMSO on oral administration. Single dose of oral DMSO administrated before irradiation conferred 100% survival of C57BL6/J mice receiving otherwise lethal as well as super-lethal radiation dose, with wide radioprotective time frame (from 15min to 4h). Oral DMSO not only protected radiation-induced acute hematopoietic stem and progenitor cell (HSPC) injury, but also ameliorated long-term BM suppression following irradiation in mice. Mechanistically, DMSO directly protected HSPC survival after irradiation in vitro and in vivo, whereas no radioprotective effect was seen in MLL-AF9-induced leukemia cells. Unexpectedly, DMSO treatment did not inhibit radiation-induced HSPC apoptosis, and the HSPC survival from Trp53-and PUMA-deficient mice after irradiation was also protected by DMSO. In conclusion, our findings demonstrate the radioprotective efficacy of oral DMSO. Given its oral efficacy and little toxicity, DMSO is an attractive candidate for human use in a wide variety of settings, including nuclear accidents and medical radiation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.freeradbiomed.2020.03.021DOI Listing
June 2020

Subspace Distribution Adaptation Frameworks for Domain Adaptation.

IEEE Trans Neural Netw Learn Syst 2020 Dec 30;31(12):5204-5218. Epub 2020 Nov 30.

Domain adaptation tries to adapt a model trained from a source domain to a different but related target domain. Currently, prevailing methods for domain adaptation rely on either instance reweighting or feature transformation. Unfortunately, instance reweighting has difficulty in estimating the sample weights as the dimension increases, whereas feature transformation sometimes fails to make the transformed source and target distributions similar when the cross-domain discrepancy is large. In order to overcome the shortcomings of both methodologies, in this article, we model the unsupervised domain adaptation problem under the generalized covariate shift assumption and adapt the source distribution to the target distribution in a subspace by applying a distribution adaptation function. Accordingly, we propose two frameworks: Bregman-divergence-embedded structural risk minimization (BSRM) and joint structural risk minimization (JSRM). In the proposed frameworks, the subspace distribution adaptation function and the target prediction model are jointly learned. Under certain instantiations, convex optimization problems are derived from both frameworks. Experimental results on the synthetic and real-world text and image data sets show that the proposed methods outperform the state-of-the-art domain adaptation techniques with statistical significance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2020.2964790DOI Listing
December 2020

Fe O nanoparticles coated with polyhedral oligomeric silsesquioxanes and β-cyclodextrin for magnetic solid-phase extraction of carbaryl and carbofuran.

J Sep Sci 2020 Apr 2;43(8):1514-1522. Epub 2020 Apr 2.

Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, P. R. China.

In this study, porous sandwich structure Fe O nanoparticles coated by polyhedral oligomeric silsesquioxanes and β-cyclodextrin were prepared by surface polymerization and were used as the magnetic solid phase extraction adsorbent for the extraction and determination of carbaryl and carbofuran. The Fe O nanoparticles coated with polyhedral oligomeric silsesquioxanes and β-cyclodextrin were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, vibrating sample magnetometry, and scanning electron microscopy. After optimizing the extraction conditions, a method that combined magnetic solid phase extraction with high-performance liquid chromatography was developed for the determination of carbaryl and carbofuran in apple. The method exhibited a good linearity in the range of 2-400 μg/kg for carbaryl and carbofuran (R = 0.9995), respectively. The limits of detection were 0.5 μg/kg of carbaryl and 0.7 μg/kg for carbofuran in apple, respectively. Extraction recoveries ranged from 94.2 to 103.1% with the preconcentration factor of 300 and the relative standard deviations were less than 5.9%. These results indicated that the method combined magnetic solid phase extraction with high-performance liquid chromatography and was promising for the determination of carbaryl and carbofuran at trace amounts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jssc.201900896DOI Listing
April 2020

Genome-wide associated study identifies NAC42-activated nitrate transporter conferring high nitrogen use efficiency in rice.

Nat Commun 2019 11 21;10(1):5279. Epub 2019 Nov 21.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, 210095, China.

Over-application of nitrogen fertilizer in fields has had a negative impact on both environment and human health. Domesticated rice varieties with high nitrogen use efficiency (NUE) reduce fertilizer for sustainable agriculture. Here, we perform genome-wide association analysis of a diverse rice population displaying extreme nitrogen-related phenotypes over three successive years in the field, and identify an elite haplotype of nitrate transporter OsNPF6.1 that enhances nitrate uptake and confers high NUE by increasing yield under low nitrogen supply. OsNPF6.1 differs in both the protein and promoter element with natural variations, which are differentially trans-activated by OsNAC42, a NUE-related transcription factor. The rare natural allele OsNPF6.1, derived from variation in wild rice and selected for enhancing both NUE and yield, has been lost in 90.3% of rice varieties due to the increased application of fertilizer. Our discovery highlights this NAC42-NPF6.1 signaling cascade as a strategy for high NUE and yield breeding in rice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-019-13187-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872725PMC
November 2019

Gene cloning, expression and homology modeling of first fibrinolytic enzyme from mushroom (Cordyceps militaris).

Int J Biol Macromol 2020 Mar 11;146:897-906. Epub 2019 Nov 11.

Heilongjiang Provincial Key Laboratory of Processing Agricultural Products, College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China; Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Fibrinolytic enzymes are important thrombolytic agents for blood-clotting disorders like cardiovascular diseases. Availability of novel recombinant fibrinolytic enzymes can overcome the shortcomings of current thrombolytic drugs. With the objective of facilitating their cost-effective production for therapeutic applications and for gaining deeper insight into their structure-function, we have cloned and expressed the first fibrinolytic protease gene from Cordyceps militaris. Cordyceps militaris fibrinolytic enzyme (CmFE) has one open reading frame of 759 bp encoding "pre-pro-protein" of 252 amino acids. Recombinant CmFE was expressed as 28 kDa extracellular enzyme in Pichia pastoris which was capable of degrading fibrin clot. A structure homology model of CmFE was developed using urokinase-type plasminogen activator. The active site contains catalytic triad His41, Asp83, Ser177 and consensus sequence of GDSGG. The substrate binding residues are Asp (171), Gly (194) and Ser (192). Its trypsin-like specificity is determined by the critical Asp171 in S1 subsite. The "oxyanion hole" is formed by backbone amide hydrogen atoms of Gly-175 and Ser-177. CmFE contains six conserved cysteines forming three disulfide linkages. This is the first study describing cloning, expression and prediction of structure-function relationship of a mushroom fibrinolytic protease. Hence it has great relevance in application of fibrinolytic enzymes as thrombolytic agents.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2019.09.212DOI Listing
March 2020

Study on the interactions between B-norcholesteryl benzimidazole compounds with ct-DNA.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Feb 31;227:117525. Epub 2019 Oct 31.

Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Key Laboratory of Beibu Gulf Environment Change and Resources Utilization, School of Chemistry and Material, Nanning Normal University, Nanning, 530001, PR China; Guangxi Colleges and University Key Laboratory of Beibu Gulf Oil and Natural Gas Resource Effective Utilization, Beibuwan University, Qinzhou, 535099, PR China. Electronic address:

The study of molecule-DNA interaction is very important for designing an improved therapeutic agent. In previous studies, we synthesized some B-norcholesteryl benzimidazole compounds, and the tests on cancer cells showed that these compounds had good in vitro anti-cancer activities. In order to further investigate mechanism of their actions, three different B-norcholesteryl benzimidazole compounds were selected and interaction of these compounds with the calf thymus DNA (ct-DNA) was monitored by using various methods including UV-Vis and fluorescence spectroscopic techniques, viscosity measurement, and circular dichroism (CD). The results proved a hypochromic effect accompanied with a slight red-shift due to the interaction of the molecules with ct-DNA. According to the UV-Vis and fluorescence spectra, the mentioned compounds were bound to DNA, preferentially through partial intercalation into the DNA helix. Moreover, the ethidium bromide (EB) and Hoechst 33258 competitive binding experiments were also used to confirm the interaction mode of the compounds with ct-DNA. In the Hoechst 33258 displacement experiment, no significant change in the fluorescence intensity was observed. Additional assays such as iodide quenching, viscosity, and CD spectroscopy further confirmed that intercalation should be the major binding mode of the selected compounds with DNA. The cytotoxicity of these three compounds was also evaluated by MTT method, and the results confirmed that binding ability of these compounds to DNA was consistent with their cytotoxicity behavior. The experimental results indicated a higher binding affinity for compound 3 compared to the other compounds. This research provided a better understanding on the molecular mechanism of the interaction between B-norcholesteryl benzimidazole compounds and tumor cells, and offered a beneficial perspective to the designation of novel B-norsteroidal anticancer compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2019.117525DOI Listing
February 2020

Highly effective remediation of Pb(II) and Hg(II) contaminated wastewater and soil by flower-like magnetic MoS nanohybrid.

Sci Total Environ 2020 Jan 6;699:134341. Epub 2019 Sep 6.

MOE Key Laboratory of Yellow River and Huai River Water Environmental and Pollution Control, Henan Key Laboratory for Environmental Pollution Control, School of Environment, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address:

The efficient enrichment and remediation of heavy metals from realistic wastewater and contaminated soil containing large excess of competitive ions remains a daunting challenge by far. In the present study, flower-like molybdenum disulfide decorated with iron oxide nanoparticles (MoS/FeO) is designed via a two-step hydrothermal method and mainly applied in the removal of Hg(II) and Pb(II) ions in aqueous environment. Exhaustive morphological, chemical and magnetic characterizations verify the successful formation of magnetic MoS/FeO. Batch adsorption experiments show that the obtained MoS/FeO nanohybrid enables efficient capture of Hg(II) and Pb(II) ions, accompanied by ease-of-separation from solution by simply applying a magnet. In this respect, high adsorption capacities (263.6 mg g for Pb(II) and 428.9 mg g for Hg(II)) can be gained under optimized conditions (pH = 5.0; 298 K; nanohybrid dosage: 0.8 g L and the contact time: 180 min). In addition, the effects of different parameters such as initial Pb(II)/Hg(II) concentration (50-500 mg L), temperature (298, 308 and 318 K) and co-existing ions (Zn(II), Cu(II), Cd(II) and Mg(II)) were systematically probed. The favorable adsorption capacity, selectivity and recyclability mainly originates from the strong Hg/Pb···S bonding interactions. Practical application potential of magnetic MoS/FeO nanohybrid in realistic lead-acid battery industry wastewater and Pb(II)-contaminated soil is further explored, achieving promising results with high Pb(II) removal efficiency of 99.63% for wastewater and 57.15% for soil. Simple preparation, easy separation and high adsorption capacity would foster thus-designed sulfide-based nanohybrid a promising adsorbent for heavy metal removal from wastewater and contaminated soil.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.134341DOI Listing
January 2020

In vitro inhalation bioaccessibility procedures for lead in PM size fraction of soil assessed and optimized by in vivo-in vitro correlation.

J Hazard Mater 2020 01 12;381:121202. Epub 2019 Sep 12.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing University, Nanjing 210023, PR China. Electronic address:

In order to assess and optimize frequently used in vitro inhalation bioaccessibility procedures for heavy metals in the inhalation risk assessment, in vivo inhalation bioavailability of Pb in simulated atmosphere fine particles (PM) from aging soils spiked with lead compounds and field soils in lead-zinc mining areas was investigated via intranasally instilled experiments with these PM suspensions to mice and Pb bioaccessibility was extracted by using four frequently used in vitro procedures (Gamble Solution, simulated lung fluid, simulated epithelial lung fluid and artificial lysosomal fluid). Mouse exposure experiments showed that Pb was mainly distributed in the liver, kidneys, blood and spleen. Based on the kidney model, in vitro inhalation bioaccessibility of Pb extracted with optimized Gamble Solution, in which solid to liquid ratio (S/L) was optimized to 1:1000 g ml and DTPA was proved to be the key effective component, showed a strong linear relationship with its in vivo inhalation bioavailability (y = 1.07x - 3.86, R = 0.73). Moreover, in vitro bioaccessible and bioavailable fractions of Pb were mainly from acid exchangeable and reducible fractions of Pb in PM. Altogether, optimized Gamble Solution was suggested for the analysis of in vitro bioaccessibility for risk-based assessments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2019.121202DOI Listing
January 2020

Glucose-functionalized near-infrared AgSe quantum dots with renal excretion ability for long-term in vivo tumor imaging.

J Mater Chem B 2019 10 4;7(38):5782-5788. Epub 2019 Sep 4.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.

Non-toxic and long-term fluorescent probes for tumor imaging are in urgent need for non-invasively obtaining information about tumor genesis and metastasis in vivo. Here, we present a biocompatible near-infrared fluorescent probe for in vivo long-term imaging of tumor by modifying glucose (Glc), which experiences high uptake in cancer cells, on the surface of near-infrared AgSe quantum dots (NIR AgSe QDs). The fluorescence of glucose-functionalized AgSe QDs (Glc-AgSe QDs) from the targeted tumor can be observed in vivo for at least 7 days. In addition, this probe could be excreted through kidneys and the renal excretion ability is favorable for in vivo imaging applications. Moreover, Glc-AgSe QDs could be used for tumor targeted imaging of not only human breast cancer cells (MCF-7), but also SW1990 pancreatic cancer cells since glucose is highly taken up in almost all kinds of tumors. Glc-AgSe QDs could be a promising general tool for in vivo long-term observation of tumor evolution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9tb01112aDOI Listing
October 2019

Enhanced elimination of non-digestible oligosaccharides from soy milk by immobilized α-galactosidase: A comparative analysis.

J Food Biochem 2019 11 8;43(11):e13005. Epub 2019 Aug 8.

Heilongjiang Provincial Key University Laboratory of Processing Agricultural Products, College of Food and Bioengineering, Qiqihar University, Qiqihar, China.

This study compared two immobilization matrices like calcium-alginate and chitosan for immobilization of α-galactosidase and evaluated their potential for the removal of non-digestible raffinose family oligosaccharides from soy milk which cause abdominal discomfort. The pH optima of the free and immobilized enzymes were found to be similar at pH 4.0. The chitosan-immobilized α-galactosidase displayed higher optimal temperature (60°C) compared to alginate-immobilized enzyme (45°C) and free enzyme (50°C). The chitosan-immobilized and alginate-immobilized α-galactosidases displayed 93.7% and 97.6% hydrolysis of raffinose family oligosaccharides, respectively, while the free enzyme hydrolyzed only 30.3% oligosaccharides present in soy milk in 4 hr. Remarkably, both the immobilized enzymes showed complete removal of raffinose family oligosaccharides in 8 hr. Moreover, reusability studies indicate that even after five cycles of reuse, the chitosan and alginate-immobilized enzymes displayed 99% and 60% hydrolysis, respectively. PRACTICAL APPLICATIONS: In this study, we have used two inexpensive and non-toxic matrices for immobilizing α-galactosidase. We report that entrapment of α-galactosidase with chitosan significantly improved the optimal temperature of α-galactosidase, which is advantageous in food industry. The hydrolysis of raffinose family oligosaccharides in soy milk was also greatly enhanced after immobilization with chitosan and alginate. Thus, the results described in this study have relevance for development of safe, cost-effective and efficient method for removal of non-digestible soy oligosaccharides in food industry.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/jfbc.13005DOI Listing
November 2019

A stable silver metallacage with solvatochromic and mechanochromic behavior for white LED fabrication.

Chem Commun (Camb) 2019 Jul 3;55(58):8474-8477. Epub 2019 Jul 3.

Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan 650091, China.

A novel luminescent metallacage was prepared by coordinating silver with benzimidazole tetraphenylethylene. It possesses high stability and strong fluorescence not only in dilute solutions but also in the aggregated state.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cc03229kDOI Listing
July 2019

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the promoter.

Theranostics 2019 13;9(9):2424-2438. Epub 2019 Apr 13.

Key Laboratory of Carcinogenesis and Invasion, Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha 410078, China.

EBV infection is a recognized epigenetic driver of carcinogenesis. We previously showed that EBV could protect cancer cells from TNF-induced necroptosis. This study aims to explore the epigenetic mechanisms allowing cancer cells with EBV infection to escape from RIP3-dependent necroptosis. : Data from the TCGA database were used to evaluate the prognostic value of promoter methylation and its expression. Western blotting, real-time PCR, and immunochemistry were conducted to investigate the relationship between LMP1 and RIP3 in cell lines and NPC tissues. BSP, MSP and hMeDIP assays were used to examine the methylation level. Induction of necroptosis was detected by cell viability assay, p-MLKL, and Sytox Green staining. : promoter hypermethylation is an independent prognostic factor of poorer disease-free and overall survival in HNSCC patients, respectively. RIP3 is down-regulated in NPC (a subtype of HNSCC). EBV(LMP1) suppresses RIP3 expression by hypermethylation of the promoter. RIP3 protein expression was inversely correlated with LMP1 expression in NPC tissues. Restoring RIP3 expression in EBV(LMP1)-positive cells inhibits xenograft tumor growth. The accumulation of fumarate and reduction of α-KG in EBV(LMP1)-positive cells led to RIP3 silencing due to the inactivation of TETs. Decreased FH activity caused fumarate accumulation, which might be associated with its acetylation. Incubating cells with fumarate protected NPC cells from TNF-induced necroptosis. : These results demonstrate a pathway by which EBV(LMP1)-associated metabolite changes inhibited necroptosis signaling by DNA methylation, and shed light on the mechanism underlying EBV-related carcinogenesis, which may provide new options for cancer diagnosis and therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.30941DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525991PMC
June 2020

Efficacy and toxicities of combination maintenance therapy in the treatment of advanced non-small-cell lung cancer: an up-to-date meta-analysis.

Biosci Rep 2019 06 14;39(6). Epub 2019 Jun 14.

The People's Hospital of Tongwei County, Dingxi, Gansu Province, China.

Single agent maintenance therapy has been approved for the treatment of advanced non-small-cell lung cancer (NSCLC) due to its potential survival benefits, but whether combined maintenance therapy would improve the survival of advanced NSCLC remains undetermined. Relevant trials were identified by searching electronic databases and conference meetings. Prospective randomized controlled trials (RCTs) assessing combination maintenance therapy in advanced NSCLC patients were included. Outcomes of interest included overall survival (OS), progression-free survival (PFS), and grade 3-4 toxicities. A total of 1950 advanced NSCLC patients received combination maintenance treatment from six trials were included for analysis. The use of doublet maintenance therapy in NSCLC patients significantly improved PFS (HR 0.74, 95%CI: 0.59-0.93, 0.010), but not for OS (HR 0.95, 95%CI: 0.85-1.07, = 0.40) in comparison with single agent maintenance therapy. Similar results were observed in sub-group analysis according to treatment regimens. In addition, there was no significantly risk difference between doublet and single agent maintenance therapy in terms of grade 3/4 hematologic and non-hematologic toxicities. The findings of the present study show that doublet combination maintenance therapy is superior to single agent maintenance therapy in terms of PFS, without increased grade 3-4 toxicities. Future prospective studies are recommended to clearly assess the long-term clinical benefit of doublet maintenance therapy and its impact on health-related quality of life.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1042/BSR20182464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6567678PMC
June 2019

IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene.

J Genet Genomics 2019 03 23;46(3):149-153. Epub 2019 Mar 23.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China; College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jgg.2019.02.006DOI Listing
March 2019

Monolithic capillary microextraction combined with ICP-MS for the determination of TiO NPs in environmental water samples.

Talanta 2019 May 11;197:334-340. Epub 2019 Jan 11.

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. Electronic address:

Increasing use of TiO nanoparticles (NPs) leads to an accumulation in environment and a risk to human. In this work, an on-line monolithic capillary microextraction (CME)-inductively coupled plasma mass spectrometry (ICP-MS) method was developed for sensitive determination of TiO NPs in environmental water samples. With the presence of citric acid, TiO NPs can be selectively adsorbed on the poly(acrylamide)-vinylpyridine-methylene bis-(acrylamide) (poly(AA-VP-Bis)) monolithic column at pH 1, whereas Ti ions cannot be adsorbed. Factors affecting the extraction efficiency such as sample pH, concentration of citric acid and elution conditions were carefully investigated. Under the optimum conditions, a detection limit of 0.63 μg L for TiO NPs and a sample throughput of 6 h were achieved. The method can be applied to the determination of TiO NPs in the size range of 5-100 nm. The developed method was then applied to the analysis of TiO NPs in the East Lake water and Yangtze River water, and recoveries of 84-109% were obtained for spiked samples. Compared with other reported methods for the determination of metal oxide NPs, the developed on-line monolithic CME-ICP-MS has the merits of simple operation, high throughput and no need for digestion before ICP-MS detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2019.01.035DOI Listing
May 2019

Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane.

BMC Plant Biol 2018 Dec 10;18(1):342. Epub 2018 Dec 10.

Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University, Ministry of Agriculture, Fuzhou, 350002, Fujian, China.

Background: Sugarcane (Saccharum spp. hybrids) is considered the most globally important sugar-producing crop and raw material for biofuel. Insect attack is a major issue in sugarcane cultivation, resulting in yield losses and sucrose content reductions. Stem borer (Diatraea saccharalis F.) causes serious yield losses in sugarcane worldwide. However, insect-resistant germplasms for sugarcane are not available in any collections all over the world, and the molecular mechanism of insect resistance has not been elucidated. In this study, cry1Ac transgenic sugarcane lines were obtained and the biological characteristics and transgene dosage effect were investigated and a global exploration of gene expression by transcriptome analysis was performed.

Results: The transgene copies of foreign cry1Ac were variable and random. The correlation between the cry1Ac protein and cry1Ac gene copies differed between the transgenic lines from FN15 and ROC22. The medium copy lines from FN15 showed a significant linear relationship, while ROC22 showed no definite dosage effect. The transgenic lines with medium copies of cry1Ac showed an elite phenotype. Transcriptome analysis by RNA sequencing indicated that up/down regulated differentially expressed genes were abundant among the cry1Ac sugarcane lines and the receptor variety. Foreign cry1Ac gene and endogenous borer stress-related genes may have a synergistic effect. Three lines, namely, A1, A5, and A6, were selected for their excellent stem borer resistance and phenotypic traits and are expected to be used directly as cultivars or crossing parents for sugarcane borer resistance breeding.

Conclusions: Cry1Ac gene integration dramatically improved sugarcane insect resistance. The elite transgenic offspring contained medium transgene copies. Foreign cry1Ac gene integration and endogenous borer stress-related genes may have a synergistic effect on sugarcane insect resistance improvement.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12870-018-1536-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288918PMC
December 2018