Publications by authors named "Xiao-Tong Wang"

97 Publications

Propensity score-matched comparison between totally laparoscopic right hemicolectomy with transcolonic natural orifice specimen extraction and conventional laparoscopic surgery with mini-laparotomy in the treatment of ascending colon cancer (with video).

Gastrointest Endosc 2021 Mar 30. Epub 2021 Mar 30.

Department of Colon and Rectal Surgery, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, People's Republic of China. Electronic address:

Background And Aims: Now that the debate about the safety and effectiveness of laparoscopic versus open surgery is over, attention has turned to innovations that can verify whether minimizing the impact of laparoscopy on the abdominal wall can further reduce pain, improve patient comfort, lead to superior cosmesis, and reduce morbidity. The aim of this study was to further explore the application value of totally laparoscopic right hemicolectomy with transcolonic natural orifice specimen extraction (NOSE) and to evaluate the short-term efficacy of transcolonic NOSE surgery for resecting specimens of ascending colon cancer.

Methods: From January 2016 to May 2017, a retrospective study was conducted in Guangxi. Propensity score matching was used to minimize the bias from nonrandomized treatment assignment. Patients were followed up through May 2020.

Results: Forty-nine patients underwent totally laparoscopic right hemicolectomy with transcolonic NOSE, and 116 patients underwent laparoscopic right hemicolectomy with mini-laparotomy (ML) procedures at our institution. After propensity score matching, each group included 45 patients, and all the covariate imbalances were alleviated. The transcolonic NOSE group and the ML group did not differ significantly in terms of baseline clinical characteristics. The transcolonic NOSE group was associated with a shorter time to first flatus (NOSE 1.8 ± 0.5 vs ML 3.2 ± 0.8, p=0.032), a shorter length of hospital stay (NOSE 11.3 ± 2.5 days vs ML 13.0 ± 3.1 days, p=0.034), a shorter time to first liquid intake (NOSE 2.6 ± 0.8 vs ML 3.8 ± 0.9, p=0.068), less pain (NOSE 1.8 ± 0.8 vs ML 4.2 ± 0.7, p=0.013), less analgesia requirement (NOSE 6 (13.3%) vs ML 21 (46.7%), p=0.001), lower CRP levels on POD1 (NOSE 3.6 ± 1.7 vs ML 8.2 ± 2.2, p=0.001) and POD3 (NOSE 2.4 ± 1.4 vs ML 4.6 ± 1.7, p=1.7 vs ML 8.2 ± 2.2, p=0.001) than the POSE group. The median follow-up was 28.4 months (interquartile range, 18.0-36.0 months). The 3-year overall survival rates were similar between the transcolonic NOSE group and the ML group.

Conclusion: In total, laparoscopic right hemicolectomy with transcolonic specimen extraction appears to be safe for selected patients with ascending colon cancer as a minimally invasive surgery.
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http://dx.doi.org/10.1016/j.gie.2021.03.028DOI Listing
March 2021

[Analysis of Performance and Microbial Diversity of ANAMMOX Sludge Based on Particle Size Differentiation].

Huan Jing Ke Xue 2021 Apr;42(4):1930-1938

Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.

In order to clarify the characteristics of anaerobic ammonia oxidizing (ANAMMOX) sludge and the succession rule of bacteria based on particle size differentiation, the performance change and microbial community structure of ANAMMOX floc sludge during the formation of particles in the reaction system of a high ammonia-nitrogen biofilter were studied. The results indicated that the specific activity (SAA) and tolerance of the ANAMMOX granular sludge (AnGS) were significantly improved by increasing the particle size, and the SAA of R4(>4.75 mm) was up to 426.8 mg·(g·d), but it also had adverse effects on mass transfer. The results of the high-throughput sequencing showed that dynamic changes between bacterial genera were common. When the particle size was less than 4.75 mm, the increase in particle size strengthened the stability of the bacterial flora, the ammonia oxidizing bacteria (AOB) with more flocs were eliminated, and the nitrogen removal ratio gradually stabilized. R3 (2.8-4.75 mm) exhibited the most specific flora composition, and the functional bacteria accounted for 52.7%, while the R4 community complexity increased. Furthermore, the proportion of functional bacteria decreased, and the abundance of heterotrophic bacteria increased, which negatively affected the particle structure. In addition, the R3 microorganism has the best gene function expression level, which is significantly better than small particles in gene replication repair and energy conversion. Finally, the evolution of AnGS was analyzed through the OTU matrix between the samples. These results have some guiding significance for the optimization of the AnGS system and will be helpful for the application of the ANAMMOX process.
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http://dx.doi.org/10.13227/j.hjkx.202009248DOI Listing
April 2021

Tunneling Nanotubes: A Novel Pharmacological Target for Neurodegenerative Diseases?

Pharmacol Res 2021 Mar 9:105541. Epub 2021 Mar 9.

State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica& Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:

Diversiform ways of intercellular communication are vital links in maintaining homeostasis and disseminating physiological states. Among intercellular bridges, tunneling nanotubes (TNTs) discovered in 2004 were recognized as potential pharmacology targets related to the pathogenesis of common or infrequent neurodegenerative disorders. The neurotoxic aggregates in neurodegenerative diseases including scrapie prion protein (PrPSc), mutant tau protein, amyloid-beta (Aβ) protein, alpha-synuclein (α-syn) as well as mutant Huntington (mHTT) protein could promote TNT formation via certain physiological mechanisms, in turn, mediating the intercellular transmission of neurotoxicity. In this review, we described in detail the skeleton, the formation, the physicochemical properties, and the functions of TNTs, while paying particular attention to the key role of TNTs in the transport of pathological proteins during neurodegeneration.
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http://dx.doi.org/10.1016/j.phrs.2021.105541DOI Listing
March 2021

Filling the Charge-Discharge Voltage Gap in Flexible Hybrid Zinc-Based Batteries by Utilizing a Pseudocapacitive Material.

Chemistry 2021 Mar 1;27(18):5796-5802. Epub 2021 Mar 1.

School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou Higher Education Mega Center, No. 230 Wai Huan Xi Road, Guangzhou, 510006, P. R. China.

The high charge-discharge voltage gap is one of the main bottlenecks of zinc-air batteries (ZABs) because of the kinetically sluggish oxygen reduction/evolution reactions (ORR/OER) on the oxygen electrode side. Thus, an efficient bifunctional catalyst for ORR and OER is highly desired. Herein, honeycomb-like MnCo O spheres were used as an efficient bifunctional electrocatalyst. It was demonstrated that both ORR and OER catalytic activity are promoted by Mn -induced oxygen vacancy defects and multiple active sites. Importantly, the multivalent ions present in the material and its defect structure endow stable pseudocapacitance within the inactive region of ORR and OER; as a result, a low charge-discharge voltage gap (0.43 V at 10 mA cm ) was achieved when it was employed in a flexible hybrid Zn-based battery. This mechanism provides unprecedented and valuable insights for the development of next-generation metal-air batteries.
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http://dx.doi.org/10.1002/chem.202100112DOI Listing
March 2021

Sleep Characteristics and Cerebrospinal Fluid Progranulin in Older Adults: The CABLE Study.

Neurotox Res 2021 Jun 14;39(3):764-773. Epub 2021 Jan 14.

Department of Neurology and Institute of Neurology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Cerebrospinal fluid (CSF) progranulin (PGRN) is related to various neurodegeneration diseases. And sleep problems can cause abnormality in protein metabolism in vivo. We aim to explore the potential associations between the self-reported sleep characteristics and CSF PGRN in cognitively intact older adults. Our study recruited 747 participants (mean (standard deviation (SD)) age, 61.99 (10.52) years, 329 (42.89%) females) who had normal cognition from the Chinese Alzheimer's Biomarker and LifestylE (CABLE) study with CSF PGRN and sleep characteristics measured. The multiple linear regression and nonlinear regression adjusted for age, gender, education, and apolipoprotein E-epsilon 4 gene (APOE4) status were used to assess the associations between sleep characteristics and PGRN. Interaction effects were explored between APOE4 status and sleep characteristics on CSF PGRN level. Sleep disturbances indicated lower CSF PGRN (β = - 0.0186, p = 0.0160). For detailed items in sleep disturbances, lower CSF PGRN was found in males who woke up during sleep (β = - 0.0121, p = 0.0062) and in females who had breathing difficulties (β = - 0.0258, p = 0.0271). Meanwhile, sleep efficiency was associated with CSF PGRN (β = - 0.0512, p = 0.0497). No significant interaction effects between sleep characteristics and APOE4 status were found. Meanwhile, we did not find a nonlinear relationship between nocturnal sleep duration and CSF PGRN. Sleep problems may influence the metabolism of PGRN, thus attenuating the protective effects of PGRN on neurodegeneration diseases.
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http://dx.doi.org/10.1007/s12640-020-00326-6DOI Listing
June 2021

Epidemiology of maxillofacial soft tissue injuries in an oral emergency department in Beijing: A two-year retrospective study.

Dent Traumatol 2021 Jun 10;37(3):479-487. Epub 2021 Jan 10.

Department of Oral Emergency, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.

Background/aims: Maxillofacial soft tissue injuries (STIs) may differ in epidemiological characteristics from other maxillofacial injuries. The aim of this study was to investigate the epidemiology of maxillofacial STIs in a public oral emergency department in Beijing, China, from 2017 to 2018.

Material And Methods: In this retrospective study, 5949 patients with maxillofacial STIs and complete medical records were evaluated. Gender, age, etiology, visit date and time, interval between accident and treatment, anatomic injury site, treatment modality, and the number of associated maxillofacial bone fractures or tooth injuries were analyzed.

Result: There were 3831 males and 2118 females (ratio 1.81:1). Patients younger than 10 years were the most frequently seen group (44.2%). Among adults, 20- to 29-years-old (16.2%) was the most prominent age group. The month of May (11.8%) had the highest incidence of maxillofacial STIs, and February (4.5%) had the lowest incidence. The average number of daily visits was significantly higher on holidays than on workdays. Of the 5949 patients, 45.9% attended at night, 2021 patients had dental trauma, and 31 had jaw fractures. The lips were the most common site of STIs, followed by the chin and gingiva. Lip and gingival STIs were more common in the 0- to 10-years-old group. Chin STIs were more common in the 20- to 39-years-old group. A fall was the leading cause of injury, especially in patients younger than 10 years and older than 70 years. Approximately 56.7% of the falls resulted in injuries to the lower one-third of the face. Sports injuries were more common among 10- to 29-years-old individuals.

Conclusion: STIs of the maxillofacial region were most likely to occur at night, in May and during holidays. Males, children younger than 10 years and 20- to 29-years-old adults were high-risk populations. Most maxillofacial STIs involved the lips, and one-third of the patients had dental trauma.
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http://dx.doi.org/10.1111/edt.12655DOI Listing
June 2021

[Effect of Land-use on Soil Fungal Community Structure and Associated Functional Group in Huixian Karst Wetland].

Huan Jing Ke Xue 2020 Sep;41(9):4294-4304

College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China.

Studying the impact of land-use on fungal communities and their functional groups in wetland soil can provide a theoretical basis for the protection of wetlands. The top soil (0-20 cm) samples were collected from the wetlands with (PCW), wetlands with (CCW), abandoned paddy fields (APF), paddy fields (PF), and corn fields (CF) in the Huixian Karst Wetland. The fungal community structure and its functional groups were analyzed using high-throughput sequencing methods and the FUNGuild database, respectively. The results showed that the Simpson and Shannon index in PF and CF were significantly higher than those in PCW and CCW. Ascomyceta was the most dominant phylum in five land-use types with the abundance of 70.60%-87.02%, followed by Rozellomycota in PCW with the abundance of 7.14% and Basidiomycota in CCW, APF, PF, and CF with the abundance of 9.70%, 5.19%, 8.13%, and 7.50%, respectively. Pleosporales was the most dominant order in PCW with the abundance of 16.47%, while Hypocreales was the dominant one in CCW, APF, PF, and CF with the abundance of 22.52%, 23.50%, 17.60, and 23.80%, respectively. and were the most dominant genera in PCW and CCW with the abundance of 6.65% and 13.44%, respectively, and was the most dominant genus in APF, PF, and CF with the abundance of 10.22%, 10.51%, and 11.12%, respectively. Saprotroph was the main trophic mode in the Huixian wetland with the abundance of 48.67%-80.13%. The abundance of pathotroph in CF (5.39%) was higher than that in PCW (2.34%) and CCW (1.53%). Dung saprotroph-wood saprotroph and soil saprotroph were the most dominant functional groups in PCW and CCW, respectively, while animal pathogen-endophyte-lichen parasite-plant pathogen-soil saprotroph-wood saprotroph was the most dominant functional group in APF, PF, and CF. Redundancy analysis showed that both soil water content and the ratio of carbon-to-nitrogen were the main factors affecting fungal communities, and available nitrogen was the main factor affecting the functional groups. Overall, the results indicated that land-use has changed the soil fungal diversity and community structure, complicated the functional groups, and increased the risk of corn disease in the Huixian Karst wetland.
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http://dx.doi.org/10.13227/j.hjkx.202001055DOI Listing
September 2020

High-Rate and Long-Cycle Cathode for Sodium-Ion Batteries: Enhanced Electrode Stability and Kinetics via Binder Adjustment.

ACS Appl Mater Interfaces 2020 Oct 6;12(42):47580-47589. Epub 2020 Oct 6.

MOE Key Laboratory for UV Light-Emitting Materials and Technology, Northeast Normal University, Changchun, Jilin 130024, P. R. China.

Sodium-ion batteries (SIBs) are heralded as promising candidates for grid-scale energy storage systems due to their low cost and abundant sodium resources. Excellent rate capacity and outstanding cycling stability are always the goals for SIBs. Up to now, nearly all attention has been focused on the control of morphology and structure of electrode materials, but the influence of binders on their performance is neglected, especially in cathode materials. Herein, using NaV(PO)OF (NVPOF) as a cathode material, the influence of four different binders (sodium alginate, SA; carboxymethylcellulose sodium, CMC; poly(vinylidene fluoride), PVDF; and poly(acrylic latex), LA133) on its electrochemical performance is studied. As a result, when using SA as the binder, the electrochemical performance of the NVPOF electrode is improved significantly, which is mainly because of the high water solubility, rich carboxyl and hydroxyl groups, and high adhesive and cohesive properties of the SA binder, leading to the uniform distribution of active materials NVPOF and carbon black in electrodes, good integrity, low polarization, and superior kinetic properties of the NVPOF electrodes, as demonstrated by scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic intermittent titration technique. More importantly, when coupled with a hard carbon anode, the fabricated sodium-ion full cells also exhibit excellent rate performance, thus providing a preview of their practical application. This work shows that the battery performance can be improved by matching suitable binder systems, which is believed to have great importance for the further optimization of the electrochemical performance of SIBs.
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http://dx.doi.org/10.1021/acsami.0c14294DOI Listing
October 2020

Associations of sleep characteristics with alpha-synuclein in cerebrospinal fluid in older adults.

Ann Clin Transl Neurol 2020 10 19;7(10):2026-2034. Epub 2020 Sep 19.

Department of Neurology and Institute of Neurology, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Objective: Sleep disorders as a preclinical symptom of synucleinopathies become more prevalent in older adults. Synucleinopathies might be caused by the abnormal aggregation of alpha-synuclein in the brain, which was indicated by alpha-synuclein levels in cerebrospinal fluid (CSF). We aimed to investigate associations of sleep characteristics with CSF alpha-synuclein in older adults.

Methods: Our study recruited 536 cognitively intact individuals (aged between 40 and 90 years old) from the Chinese Alzheimer's Biomarker and Lifestyle study. Sleep behaviors were assessed by Pittsburgh Sleep Quality Index and total alpha-synuclein in CSF was measured by enzyme-linked immune-sorbent assay. We used multiple linear and non-linear regression models for research.

Results: Significant non-linear associations of CSF alpha-synuclein with sleep time and duration were revealed. Individuals who went to bed and fell asleep too early or late tended to have lower CSF alpha-synuclein (reflection point for time to bed and fall asleep were 10:26 p.m. and 10:40 p.m.). Lower CSF alpha-synuclein was also observed in individuals with either excessive or insufficient sleep duration (reflection point: 7.24 hours). Besides, overall poor sleep quality (β = -0.0621; P = 0.0242), longer sleep latency (β = -0.0415; P = 0.0174) and lower sleep efficiency (β = 0.0036; P = 0.0017) showed linear associations with lower CSF alpha-synuclein. Sleep disturbances and daytime dysfunction were not significantly associated with CSF alpha-synuclein.

Interpretation: Poor sleep was associated with lower levels of CSF alpha-synuclein in older adults, which may provide new insight into the prevention of synucleinopathies.
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http://dx.doi.org/10.1002/acn3.51204DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545588PMC
October 2020

Lentivirus-Mediated Overexpression of SIVA-1 Reverses Cisplatin Resistance in Gastric Cancer in vitro.

Cell Biochem Biophys 2020 Dec 9;78(4):455-463. Epub 2020 Jul 9.

Department of Gastrointestinal and Peripheral Vascular Surgery, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.

SIVA-1 plays a critical role in the induction of apoptosis in a number of different cell lines and participates in the mechanism of cisplatin (DDP)-mediated antitumor effects. However, the involvement of SIVA-1 in cisplatin resistance in gastric carcinoma has not been revealed. To explore the effect of SIVA-1 on DDP resistance, a recombinant pGV358-GFP-SIVA-1 lentiviral vector was constructed and transfected into human cisplatin-resistant MKN45/DDP gastric cancer cells. Subsequently, stable SIVA-1 overexpression was established in MKN45/DDP cells, which resulted in increased DDP sensitivity in MKN45/DDP cells in vitro. Flow cytometry demonstrated that SIVA-1 overexpression increased the percentage of apoptotic cells compared to that in the control. The colony formation assay clearly revealed that cell growth and proliferation were significantly suppressed following SIVA-1 overexpression. In addition, overexpression of SIVA-1 inhibited the migratory and invasive potential of MKN45/DDP cells in vitro. Western blot analysis indicated that SIVA-1 increased the expression levels of p53, p73, and p14ARF, whereas it reduced Bcl-2, MDM2, and Bcl-xL expression. In short, SIVA-1 upregulated the protein expression of p53, p73, and p14ARF and decreased that of Bcl-2, MDM2, and Bcl-xL in vitro and subsequently reversed cisplatin resistance in gastric cancer cells, suggesting that SIVA-1 serves as a valuable potential target for attenuating chemotherapy resistance.
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http://dx.doi.org/10.1007/s12013-020-00929-yDOI Listing
December 2020

Siva‑1 regulates multidrug resistance of gastric cancer by targeting MDR1 and MRP1 via the NF‑κB pathway.

Mol Med Rep 2020 Aug 3;22(2):1558-1566. Epub 2020 Jun 3.

Department of Gastrointestinal and Peripheral Vascular Surgery, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Siva‑1 is a well‑known anti‑apoptosis protein that serves a role in multiple types of cancer cells. However, whether Siva‑1 affects multidrug resistance via the NF‑κB pathway in gastric cancer is currently unknown. The present study aimed to determine the possible involvement of Siva‑1 in gastric cancer anticancer drug resistance in vitro. A vincristine (VCR)‑resistant KATO III/VCR gastric cancer cell line with stable Siva‑1 overexpression was established. The protein expression levels of Siva‑1, NF‑κB, multidrug resistance 1 (MDR1) and multidrug resistance protein 1 (MRP1) were detected via western blotting. The effect of Siva‑1 overexpression on anticancer drug resistance was assessed by measuring the 50% inhibitory concentration of KATO III/VCR cells to VCR, 5‑fluorouracil and doxorubicin. The rate of doxorubicin efflux and apoptosis were detected by flow cytometry. Additionally, colony formation, wound healing and Transwell assays were used to detect the proliferation, migration and invasion of cells, respectively. The results of the current study revealed that the Siva‑1‑overexpressed KATO III/VCR gastric cancer cells exhibited a significantly decreased sensitivity to VCR, 5‑fluorouracil and doxorubicin. The results of flow cytometry revealed that the percentage of apoptotic cells decreased following overexpression of Siva‑1. The colony formation assay demonstrated that cell growth and proliferation were significantly promoted by Siva‑1 overexpression. Additionally, Siva‑1 overexpression increased the migration and invasion of KATO III/VCR cells in vitro. Western blot analysis determined that Siva‑1 overexpression increased NF‑κB, MDR1 and MRP1 levels. The current study demonstrated that overexpression of Siva‑1, which functions as a regulator of MDR1 and MRP1 gene expression in gastric cancer cells via promotion of NF‑κB expression, inhibited the sensitivity of gastric cancer cells to certain chemotherapies. These data provided novel insight into the molecular mechanisms of gastric cancer, and may be of significance for the clinical diagnosis and therapy of patients with gastric cancer.
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http://dx.doi.org/10.3892/mmr.2020.11211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7339453PMC
August 2020

[Fast Start-Up ANAMMOX Operation Strategy and Flora Characteristics of a Biofilter].

Huan Jing Ke Xue 2020 Jul;41(7):3345-3355

Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.

To achieve the rapid start-up of anaerobic ammonia oxidation (ANAMMOX) reaction at room temperature and sludge granulation, a small amount of ANAMMOX sludge was inoculated by an upflow biological filter reactor, and ANAMMOX was started by stage variable load and shortening hydraulic residence time (HRT) operation strategy. The reaction was evaluated, and the nitrogen removal characteristics of the biofilter were evaluated. The results showed that the biofilter started the ANAMMOX reaction in a medium-temperature (25-29℃) environment for 22 days. After 97 days of culture, the total nitrogen volume removal rate (NRR) reached 5.64 kg·(m·d), the total nitrogen (TN) removal rate was close to 80%, and the average particle size of the granular sludge was 4.5 mm. High-throughput sequencing analysis showed that the biofilter formed a layered structure of nitrifying bacteria-heterotrophic bacteria, anaerobic ammonium oxidation bacteria (AAOB), and AAOB-heterotrophic bacteria from bottom to top. The various microflora synergistically denitrified, creating a low dissolved oxygen (DO) stable environment for AAOB, and the dominant population (AF375995.1) was enriched. In addition, the removal process of pollutants along the path and the characteristics of sludge along the process were analyzed, and it was verified that the sludge in the reaction zone had good ANAMMOX activity. An ANAMMOX-biofilter reactor achieves rapid start-up of the ANAMMOX reaction, sludge granulation, and efficient operation by effectively maintaining the amount of bacteria and stabilizing the reaction conditions.
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http://dx.doi.org/10.13227/j.hjkx.201912112DOI Listing
July 2020

[Environmental Factors Influence and Microbial Community Structure Analysis of Entrapped Anaerobic Ammonium Oxidizing Bacteria].

Huan Jing Ke Xue 2020 Feb;41(2):839-848

Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.

To solve the problems of a slow start, easy loss, and easily inhibited activity of the Anammox bacteria suspension culture, polyvinyl alcohol-polypropylene (PVA-PP) was used to prepare the Anammox immobilized filler. To improve the activity of Anammox bacteria and ensure stable operation of the reaction system, the effects of COD interference, change in pH value, and rotating speed on the nitrogen removal characteristics of the immobilized filler were determined in batch tests. Changes in the structure and diversity of the bacteria in the filler were analyzed by a high-throughput sequencing technique. The results showed that the activity of Anammox bacteria could recover to 100% on the 30th day, and the total nitrogen removal rate was 87.7% when the total nitrogen volume load (NLR) was 0.69 kg·(m·d) at the stage of 99 days. After 140 days of long-term operation, the total nitrogen removal rate (NRR) reached 1.83 kg·(m·d), which was 9.4 times the suspended sludge before immobilization. The diversity of the population was maintained in the inclusion carrier, and the effective enrichment of (AF375995.1), which performs anaerobic ammonia-oxidization, increased from 11.06% to 32.55%. The influence of COD interference and changes in the pH value of Anammox bacteria was significantly weakened, and the PVA-PP entrapped carrier could achieve the coupling removal of nitrogen by Anammox and denitrification. Appropriate external hydraulic disturbance would promote the Anammox reaction in immobilized systems.
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http://dx.doi.org/10.13227/j.hjkx.201908157DOI Listing
February 2020

sp. nov., isolated from the production water of a water-flooded oil reservoir in PR China.

Int J Syst Evol Microbiol 2020 Jul;70(7):4364-4371

State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

A novel, moderately thermophilic, Gram-stain-negative bacterium, designated strain J18, was isolated from a water-flooded oil reservoir. Cells were aerobic, oxidase- and catalase-positive, with a polar flagellum. Growth occurred at 35-60 °C and at pH 6-8.5. The respiratory quinones were ubiquinone 8 and ubiquinone 9. The dominant cellular fatty acids were C, C cyclo, C cyclo 8 and summed feature 8 (C7/C6). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, an unidentified phospholipid and an unidentified aminophospholipid. The strain showed the highest 16S rRNA gene sequence similarities to DSM 15129 (98.6 %), DSM 15512 (98.4 %) and DSM 27220 (98.1 %), respectively, and the similarity to other species was lower than 93 %. In the phylogenetic trees, it constituted a unique sub-cluster within the genus . The DNA G+C content of strain J18 was 64.44 mol%. As compared with the type strains, the genome-to-genome distances of strain J18 were 34.7-40 %. These results confirmed the separate species status of J18 with its close relatives. On the basis of physiological, chemotaxonomic and phylogenetic analyses along with the low levels of identity at the whole-genome level, it can be concluded that strain J18 represents a new species of the genus , for which the name sp. nov. is proposed. The type strain of is J18 (=CGMCC 1.16800=LMG 31400).
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http://dx.doi.org/10.1099/ijsem.0.004297DOI Listing
July 2020

Regeneration of Dental Pulp Tissue by Autologous Grafting Stem Cells Derived from Inflammatory Dental Pulp Tissue in Immature Premolars in a Beagle Dog.

Chin J Dent Res 2020 ;23(2):143-150

Objective: To compare the biological characteristics of dental pulp stem cells (DPSCs) and inflamed dental pulp derived stem cells (I-DPSCs) in vitro and their regeneration potential in Beagle immature premolars.

Methods: Pulpitis was induced in the premolars of one beagle dog by opening the pulp chamber for 2 weeks, and inflammation was histologically confirmed. DPSCs and I-DPSCs were isolated from normal and inflamed dental pulp, and cell morphology, expression of mesenchymal stem cell markers, clone formation ability, cell proliferation and osteogenic/odontogenic differentiation potential were compared. The dental pulp of 20 roots from 10 immature premolars was extracted and divided into two groups. DPSCs or I-DPSCs with scaffolds were transplanted into the root canals. The roots were extracted after 3 months, and pulp regeneration was evaluated by histological analysis. The data were statistically analysed using one-way ANOVA and a Student t test.

Results: Histological analyses showed lymphocyte infiltration and elevated TNF-α expression, which confirmed the diagnosis of pulpitis. I-DPSCs showed similar morphology, marker gene expression and clone formation ability but greater proliferation ability and osteogenic/odontogenic differentiation potential. Pulp-like tissue formation and bone- and dentine-like tissue deposition were observed in both DPSC- and I-DPSC-transplanted roots.

Conclusion: DPSCs derived from inflammatory dental pulp tissue have similar biological characteristics to those from normal dental pulp and could mediate pulp and dentine regeneration in immature premolars.
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http://dx.doi.org/10.3290/j.cjdr.a44750DOI Listing
June 2020

Regulation of Cathode-Electrolyte Interphase via Electrolyte Additives in Lithium Ion Batteries.

Chem Asian J 2020 Sep 7;15(18):2803-2814. Epub 2020 Jul 7.

National & Local United Engineering Laboratory for Power Batteries, Faculty of Chemistry, Northeast Normal University, Changchun, Jilin 130024, P.R. China.

As the power supply of the prosperous new energy products, advanced lithium ion batteries (LIBs) are widely applied to portable energy equipment and large-scale energy storage systems. To broaden the applicable range, considerable endeavours have been devoted towards improving the energy and power density of LIBs. However, the side reaction caused by the close contact between the electrode (particularly the cathode) and the electrolyte leads to capacity decay and structural degradation, which is a tricky problem to be solved. In order to overcome this obstacle, the researchers focused their attention on electrolyte additives. By adding additives to the electrolyte, the construction of a stable cathode-electrolyte interphase (CEI) between the cathode and the electrolyte has been proven to competently elevate the overall electrochemical performance of LIBs. However, how to choose electrolyte additives that match different cathode systems ideally to achieve stable CEI layer construction and high-performance LIBs is still in the stage of repeated experiments and exploration. This article specifically introduces the working mechanism of diverse electrolyte additives for forming a stable CEI layer and summarizes the latest research progress in the application of electrolyte additives for LIBs with diverse cathode materials. Finally, we tentatively set forth recommendations on the screening and customization of ideal additives required for the construction of robust CEI layer in LIBs. We believe this minireview will have a certain reference value for the design and construction of stable CEI layer to realize desirable performance of LIBs.
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http://dx.doi.org/10.1002/asia.202000522DOI Listing
September 2020

Malignant melanotic Xp11 neoplasms exhibit a clinicopathologic spectrum and gene expression profiling akin to alveolar soft part sarcoma: a proposal for reclassification.

J Pathol 2020 08 2;251(4):365-377. Epub 2020 Jul 2.

Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing, PR China.

The classification of the distinct group of mesenchymal neoplasms, first described as 'Xp11 translocation perivascular epithelioid cell tumor (PEComa)' and for which the term 'melanotic Xp11 neoplasm' or 'Xp11 neoplasm with melanocytic differentiation' has recently been proposed, remains challenging and controversial. We collected 27 melanotic Xp11 neoplasms, the largest series to date, for a comprehensive evaluation. Fourteen of the cases, together with eight alveolar soft part sarcomas (ASPS), nine conventional PEComas and a control group of seven normal tissues were submitted to RNA sequencing. Follow-up available in 22 patients showed 5-year overall survival and 5-year disease-free survival of 47.6 and 35.7%, respectively, which were similar to ASPS and significantly worse than conventional PEComa. Univariate analysis of location (occurring in the kidney versus not kidney), infiltrative growth pattern, nuclear pleomorphism, mitotic activity ≥2/50 high-power fields (HPF), necrosis and lymphovascular invasion were found to be associated with overall survival and/or disease-free survival. Multivariate analysis identified that location was the only factor found to independently correlate with disease-free survival. More importantly, RNA sequencing-based clustering analysis segregated melanotic Xp11 neoplasm and ASPS from other tumors, including conventional PEComa and Xp11 translocation renal cell carcinoma, and formed a compact cluster representative of the largely similar expression signature. Here we clearly define the true biologic nature of melanotic Xp11 neoplasms which are distinctive malignant mesenchymal tumors, rather than simply PEComa variants with occasionally unpredictable behavior. Meanwhile, melanotic Xp11 neoplasm and ASPS more likely represent phenotypic variants of the same entity, which is distinct from conventional PEComa and Xp11 translocation renal cell carcinoma. Based on these important findings, melanotic Xp11 neoplasm might be reclassified into a distinctive entity together with ASPS, independent from PEComa, in future revisions of the current WHO categories of tumors of soft tissue and bone for the improved reclassification. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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http://dx.doi.org/10.1002/path.5470DOI Listing
August 2020

Mitochondrial genomes of three Bostrichiformia species and phylogenetic analysis of Polyphaga (Insecta, Coleoptera).

Genomics 2020 09 15;112(5):2970-2977. Epub 2020 May 15.

State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, People's Republic of China. Electronic address:

Here we determined mitogenomes of three Bostrichiformia species. These data were combined with 51 previously sequenced Polyphaga mitogenomes to explore the higher-level relationships within Polyphaga by using four different mitogenomic datasets and three tree inference approaches. Among Polyphaga mitogenomes we observed heterogeneity in nucleotide composition and evolutionary rates, which may have affected phylogenetic inferences across the different mitogenomic datasets. Elateriformia, Cucujiformia, and Scarabaeiformia were each inferred to be monophyletic by all analyses, as was Bostrichiformia by most analyses based on two datasets with low heterogeneity. The large series Staphyliniformia was never recovered as monophyletic in our analyses. The Bayesian tree using a degenerated nucleotide dataset (P123_Degen) and a site-heterogeneous mixture model in PhyloBayes was supported as the best Polyphaga phylogeny: (Scirtiformia, (Elateriformia, ((Bostrichiformia, Cucujiformia), (Scarabaeiformia + Staphyliniformia)))). For Cucujiformia, the largest series, we inferred a superfamily-level phylogeny: ((Cleroidea, Coccinelloidea), (Tenebrionoidea, (Cucujoidea + Curculionoidea + Chrysomeloidea))).
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http://dx.doi.org/10.1016/j.ygeno.2020.05.012DOI Listing
September 2020

gen. nov., sp. nov., a new member of the family isolated from oil reservoir water.

Int J Syst Evol Microbiol 2020 May;70(5):3468-3474

State Key Laboratory of Agrobiotechnology, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.

A novel Gram-staining-negative, spiral-shaped bacterium, designated strain 64-1, was isolated from oil reservoir water collected from Liaohe oilfield, north-eastern China. Growth occurred at 15-55 °C and pH 6.0-10.0. The sole respiratory quinone was Q-10. The predominant cellular fatty acids were summed feature 8 (C7 /C6), C and C cyclo 8. The polar lipids consisted of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), an unidentified aminophospholipid (UAPL), an unidentified aminolipid (UAL) and two unidentified polar lipids (UPL). The genomic DNA G+C content of strain 64-1 was 64.5 mol%. Strain 64-1 shared the highest 16S rRNA gene sequence similarities with JA145 (92.0 %) and 26-4b1 (91.8 %). In the phylogenetic trees, the strain constituted a sub-cluster within the family . Based on the results of morphological, physiological, biochemical and phylogenetic analysis, strain 64-1 represents a new species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is 64-1 (=CGMCC 1.16798=LMG 31399).
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http://dx.doi.org/10.1099/ijsem.0.004200DOI Listing
May 2020

Coupling Magnetic Single-Crystal Co Mo O with Ultrathin Nitrogen-Rich Carbon Layer for Oxygen Evolution Reaction.

Angew Chem Int Ed Engl 2020 Jul 18;59(29):11948-11957. Epub 2020 May 18.

School of Chemistry and Chemical Engineering/, Institute of Clean Energy and Materials/, Guangzhou Key Laboratory for Clean Energy and Materials/, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou Higher Education Mega Center, No. 230 Wai Huan Xi Road, Guangzhou, 510006, P. R. China.

Transition-metal oxides as electrocatalysts for the oxygen evolution reaction (OER) provide a promising route to face the energy and environmental crisis issues. Although palmeirite oxide A Mo O as OER catalyst has been explored, the correlation between its active sites (tetrahedral or octahedral) and OER performance has been elusive. Now, magnetic Co Mo O @NC-800 composed of highly crystallized Co Mo O nanosheets and ultrathin N-rich carbon layer is shown to be an efficient OER catalyst. The catalyst exhibits favorable performance with an overpotential of 331 mV@10 mA cm and 422 mV@40 mA cm , and a full water-splitting electrolyzer with it as anode catalyst shows a cell voltage of 1.67 V@10 mA cm in alkaline. Combined HAADFSTEM, magnetic, and computational results show that factors influencing the OER performance can be attributed to the tetrahedral Co sites (high spin, t e ), which improve the OER kinetics of rate-determining step to form *OOH.
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http://dx.doi.org/10.1002/anie.202004533DOI Listing
July 2020

Nanostructures for Electrocatalytic CO Reduction.

Chemistry 2020 Nov 17;26(62):14024-14035. Epub 2020 Sep 17.

School of Chemistry and Chemical Engineering, Institute of, Clean Energy and Materials, Guangzhou Key Laboratory for, Clean Energy and Materials, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, No. 230 Wai Huan Xi Road, Guangzhou Higher, Education Mega Center, 510006, Guangzhou, P. R. China.

One of the most effective ways to cope with the problems of global warming and the energy shortage crisis is to develop renewable and clean energy sources. To achieve a carbon-neutral energy cycle, advanced carbon sequestration technologies are urgently needed, but because CO is a thermodynamically stable molecule with the highest carbon valence state of +4, this process faces many challenges. In recent years, electrochemical CO reduction has become a promising approach to fix and convert CO into high-value-added fuels and chemical feedstock. However, the large-scale commercial use of electrochemical CO reduction systems is hindered by poor electrocatalyst activity, large overpotential, low energy conversion efficiency, and product selectivity in reducing CO . Therefore, there is an urgent need to rationally design highly efficient, stable, and scalable electrocatalysts to alleviate these problems. This minireview also aims to classify heterogeneous nanostructured electrocatalysts for the CO reduction reaction (CDRR).
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http://dx.doi.org/10.1002/chem.202000692DOI Listing
November 2020

Mitogenome evolution in ladybirds: Potential association with dietary adaptation.

Ecol Evol 2020 Jan 2;10(2):1042-1053. Epub 2020 Jan 2.

Collaborative Innovation Center for Modern Grain Circulation and Safety College of Food Science and Engineering Nanjing University of Finance and Economics Nanjing China.

Dietary shifts can alter the relative availability of different nutrients and are therefore associated with metabolic adaptation in animals. The Coccinellidae (ladybirds) exhibits three major types of feeding habits and provides a useful model to study the effects of dietary changes on the evolution of mitogenomes, which encode proteins directly involved in energy metabolism. Here, mitogenomes of three coccinellid species were newly sequenced. These data were combined with other ten previously sequenced coccinellid mitogenomes to explore the relationship between mitogenome evolution and diets. Our results indicate that mitogenomic data can be effectively used to resolve phylogenetic relationships of Coccinellidae. Strong codon usage bias in coccinellid mitogenomes was predominantly determined by nucleotide composition. The 13 mitochondrial protein-coding genes (PCGs) globally evolved under negative constraints, with some PCGs showing a stronger purifying selection. Six PCGs (, and from Complex I; and from Complex IV; and from Complex V) displayed signs of positive selection. Of these, adaptive changes in were potentially associated with metabolic differences resulting from dietary shifts in Coccinellidae. Our results provide insights into the adaptive evolution of coccinellid mitogenomes in response to both dietary shifts and other life history traits.
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http://dx.doi.org/10.1002/ece3.5971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988538PMC
January 2020

Surface Reorganization on Electrochemically-Induced Zn-Ni-Co Spinel Oxides for Enhanced Oxygen Electrocatalysis.

Angew Chem Int Ed Engl 2020 Apr 28;59(16):6492-6499. Epub 2020 Feb 28.

School of Chemistry and Chemical Engineering/Guangzhou Key Laboratory for Clean Energy and Materials/Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, No. 230 Wai Huan Xi Road, Guangzhou, 510006, P. R. China.

Herein, we highlight redox-inert Zn in spinel-type oxide (Zn Ni Co O ) to synergistically optimize physical pore structure and increase the formation of active species on the catalyst surface. The presence of Zn segregation has been identified experimentally and theoretically under oxygen-evolving condition, the newly formed V -O-Co allows more suitable binding interaction between the active center Co and the oxygenated species, resulting in superior ORR performance. Moreover, a liquid flow Zn-air battery is constituted employing the structurally optimized Zn Ni Co O nanoparticles supported on N-doped carbon nanotube (ZNCO/NCNTs) as an efficient air cathode, which presents remarkable power density (109.1 mW cm ), high open circuit potential (1.48 V vs. Zn), excellent durability, and high-rate performance. This finding could elucidate the experimentally observed enhancement in the ORR activity of Zn Ni Co O oxides after the OER test.
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http://dx.doi.org/10.1002/anie.202000690DOI Listing
April 2020

[Subsequent Effects of Slag and Biochar Application on Greenhouse Gas Emissions from Paddy Fields in the Fuzhou Plain].

Huan Jing Ke Xue 2020 Jan;41(1):489-498

Institute of Geography, Fujian Normal University, Fuzhou 350007, China.

We investigate whether slag and biochar applications have subsequent effects on greenhouse gas emissions from paddy fields by applying biochar (B), slag (S), and a biochar-slag mix (BS) to paddy fields in the Fuzhou Plain, China. Applications of the three treatments along with a control (CK) of no amendment were made in 2015 before early and late rice seedlings were transplanted. Two years later in 2017, the CO, CH, and NO emissions in the different treatments and control were measured in the early and late rice growing seasons. The results showed that, in the rice growing season, the averaged CO emission in the control, biochar, slag, and mixed applications were (1723.66±194.56), (1245.52±155.05), (1140.29±79.68), and (1055.83±62.13) mg·(m·h), respectively. The CO emissions from the three treatments were significantly lower than the control group (<0.05), and the reduction ratios of each treatment to the control were 27.74%, 33.84%, and 38.75%, respectively. The averaged CH emissions in the control, biochar, slag, and mixed applications were (0.45±0.03), (0.40±0.05), (0.36±0.10), and (0.25±0.04) mg·(m·h), respectively, which were lower, but not significantly so (>0.05), than the control. The ratios of CH emissions from each treatment to the control were 11.11%, 20.00%, and 44.44%, respectively. The averaged NO emissions from the control, biochar, slag, and mixed applications were (62.47±27.00), (115.09±30.94), (79.75±24.98), and (112.68±23.59) μg·(m·h), respectively. In comparison to the control, the biochar, slag, and mixed treatments increased the NO emissions by 84.23%, 27.66%, and 80.37%, respectively. The global comprehensive warming potential indicated that the application treatments increased the comprehensive warming potential of the early and late rice paddy ecosystems; after 2 years of applying slag and biochar treatments, their effect on the emission reductions were not obvious.
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http://dx.doi.org/10.13227/j.hjkx.201906217DOI Listing
January 2020

Iridium-catalyzed B-H insertion of sulfoxonium ylides and borane adducts: a versatile platform to α-boryl carbonyls.

Chem Commun (Camb) 2020 Jan;56(3):423-426

Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, 510006, China.

Iridium-catalyzed boron-hydrogen bond insertion reactions of trimethylamine-borane and sulfoxonium ylides have been demonstrated, furnishing α-boryl ketones in moderate to excellent yields in most cases (51 examples; up to 84%). This practical and scalable insertion reaction showed broad substrate scope, high functional-group compatibility and could be applied in late-stage modification of structurally complex drug compounds. Further synthetic applications were also demonstrated.
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http://dx.doi.org/10.1039/c9cc08795hDOI Listing
January 2020

Gene Polymorphisms Are Associated With the Therapeutic Responses to Repaglinide in Chinese Patients With Type 2 Diabetes Mellitus.

Front Pharmacol 2019 7;10:1318. Epub 2019 Nov 7.

Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China.

The objective of this study was to investigate whether gene variants influence repaglinide response in Chinese patients with newly diagnosed type 2 diabetes mellitus (T2DM). A total of 300 patients with T2DM and 200 control subjects were enrolled to identify rs10830963 and rs1387153 genotypes by real-time polymerase chain reaction (PCR), with subsequent high-resolution melting (HRM) analysis. Ninety-five patients with newly diagnosed T2DM were randomly selected to undergo 8 weeks of repaglinide treatment (3 mg/day). After 8-week repaglinide monotherapy, patients with at least one G allele of rs10830963 showed a smaller decrease in fasting plasma glucose (FPG) ( = 0.031) and a smaller increase in homeostasis model assessment for beta cell function (HOMA-B) ( = 0.002) levels than those with the CC genotype did. The T allele carriers at rs1387153 exhibited a smaller decrease in FPG ( = 0.007) and smaller increases in postprandial serum insulin (PINS) ( = 0.016) and HOMA-B ( < 0.001) levels compared to individuals with the CC genotype. These data suggest that the rs10830963 and rs1387153 polymorphisms are associated with repaglinide monotherapy efficacy in Chinese patients with T2DM.
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http://dx.doi.org/10.3389/fphar.2019.01318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6855210PMC
November 2019

Clinicopathologic and Molecular Analysis of the TFEB Fusion Variant Reveals New Members of TFEB Translocation Renal Cell Carcinomas (RCCs): Expanding the Genomic Spectrum.

Am J Surg Pathol 2020 04;44(4):477-489

Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province.

Xp11 renal cell carcinoma (RCC) with different gene fusions may have different clinicopathologic features. We sought to identify variant fusions in TFEB translocation RCC. A total of 31 cases of TFEB RCCs were selected for the current study; MALAT1-TFEB fusion was identified in 25 cases (81%, 25/31) using fusion probes. The remaining 6 cases (19%, 6/31) were further analyzed by RNA sequencing and 5 of them were detected with TFEB-associated gene fusions, including 2 ACTB-TFEB, 1 EWSR1-TFEB, 1 CLTC-TFEB, and 1 potential PPP1R10-TFEB (a paracentric inversion of the TFEB gene, consistent with "negative" TFEB split FISH result, and advising a potential diagnostic pitfall in detecting TFEB gene rearrangement). Four of the 5 fusion transcripts were successfully validated by reverse transcription-polymerase chain reaction and Sanger sequencing. Morphologically, approximately one third (29%, 9/31) of TFEB RCCs showed typical biphasic morphology. The remaining two thirds of the cases (71%, 22/31) exhibited nonspecific morphology, with nested, sheet-like, or papillary architecture, resembling other types of renal neoplasms, such as clear cell RCC, Xp11 RCC, perivascular epithelioid cell tumor (PEComa), or papillary RCC. Although cases bearing a MALAT1-TFEB fusion demonstrated variable morphologies, all 9 cases featuring typical biphasic morphology were associated with MALAT1-TFEB genotype. Accordingly, typical biphasic morphology suggests MALAT1-TFEB fusion, whereas atypical morphology did not suggest the specific type of fusion. Isolated or clustered eosinophilic cells were a common feature in TFEB RCCs, which may be a useful morphology diagnostic clue for TFEB RCCs. Clinicopathologic variables assessment showed that necrosis was the only morphologic feature that correlated with the aggressive behavior of TFEB RCC (P=0.004). In summary, our study expands the genomic spectrum and the clinicopathologic features of TFEB RCCs, and highlights the challenges of diagnosis and the importance of subtyping of this tumor by combining morphology and multiple molecular techniques.
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http://dx.doi.org/10.1097/PAS.0000000000001408DOI Listing
April 2020

Surgical-related risk factors associated with anastomotic leakage after resection for rectal cancer: a meta-analysis.

Jpn J Clin Oncol 2020 Jan;50(1):20-28

Departments of Gastrointestinal and Peripheral Vascular Surgery, People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, People's Republic of China.

Objectives: Anastomotic leakage (AL) after anterior resection always leads to longer hospital stays, decreased quality of life and even increased mortality. Despite extensive research, no consensus on the world well-concerned surgical-related risk factors exists. We therefore conducted a meta-analysis of the available published literature to identify the effects of surgical-related risk factors for AL after anterior resection for rectal cancer, hoping to provide more information and improved guidance for clinical workers managing patients with rectal cancer who are at a high risk for AL.

Methods: In this study, the relevant articles were systematically searched from EMBASE, MEDLINE, PubMed, WangFang (Database of Chinese Ministry of Science & Technology), Chinese National Knowledge Infrastructure Database and China Biological Medicine Database. The pooled odds ratio (OR) with 95% confidence interval (95% CI) were calculated. Meta-analysis was performed using of RevMan 5.3 software.

Results: A total of 26 studies met the inclusion criteria and comprised 34238 cases. Analysis of these 26 studies showed that no defunctioning stoma was highly correlated with AL (pooled OR = 1.28, 95%CI: 1.05-1.57, P = 0.01, random effect), and intraoperative blood transfusion was significantly associated with AL (pooled OR = 1.64, 95%CI: 1.34-2.02, P = 0.02, random effect). However, the AL was not associated with type of anastomosis, type of surgery, technique of anastomosis, level of inferior mesenteric artery ligation, operation time and splenic flexure mobilization.

Conclusions: Depend on this meta-analysis, no defunctioning stoma and intraoperative blood transfusion are the major surgical-related risk factors for AL after resection for rectal cancer. Because of the inherent limitations of the research, future prospective randomized controlled trials will need to confirm this conclusion.
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http://dx.doi.org/10.1093/jjco/hyz139DOI Listing
January 2020

Targeted next-generation sequencing revealed distinct clinicopathologic and molecular features of VCL-ALK RCC: A unique case from an older patient without clinical evidence of sickle cell trait.

Pathol Res Pract 2019 Nov 17;215(11):152651. Epub 2019 Sep 17.

Department of Pathology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, Jiangsu, China. Electronic address:

Anaplastic lymphoma kinase (ALK)-rearranged renal cell carcinoma (RCC) is a novel entity of rare tumors with only 10 cases reported in the literature. Three RCC cases bearing VCL-ALK gene fusion were all young African American patients and associated with sickle cell trait notably. In contrast to the 3 reported cases, this neoplasm occurred in a middle-age woman (57 years old) without any evidence of sickle cell trait and demonstrated an infiltrating growth pattern with tubular, tubulopapillary, and tubulocystic structures, overlapping with collecting duct carcinoma and renal medullary carcinoma. Abundant intraluminal mucin was also noted significantly in the histologic sections. Immunostaining showed strong membranous labeling for ALK protein. We applied a large panel-targeted next-generation sequencing to explore the molecular alterations in the current case, revealing a driver oncogene VCL-ALK gene fusion co-occurring with pathogenic mutations in EP300 and TRRAP genes. Thereafter, fluorescence in situ hybridization assay was used to detect the ALK gene rearrangement. Reverse transcription polymerase chain reaction confirmed the presence of a VCL-ALK gene fusion, a fusion of VCL exon 16 to ALK exon 20. Our report draws the attention to the possibility that VCL-ALK genotype can be involved in older patients unassociated with sickle cell trait, also expanding the spectrum to ALK-rearranged RCC.
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http://dx.doi.org/10.1016/j.prp.2019.152651DOI Listing
November 2019

Chinese medicine Yu-Ping-Feng-San attenuates allergic inflammation by regulating epithelial derived pro-allergic cytokines.

Chin J Nat Med 2019 Jul;17(7):525-534

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210046, China. Electronic address:

This study aimed to investigate the mechanisms of Yu-Ping-Feng-San (YPFS) on attenuating allergic inflammation in the initial stage of atopic dermatitis (AD). AD mouse model was established with fluorescein isothiocyanate (FITC) sensitization and elicitation. Epithelial barrier structure was observed with transmission electron microscope. The populations of dendritic cells (DCs) and group 2 innate lymphoid cells (ILC2s) were detected by flow cytometry. Human immortalized keratinocyte (HaCaT) cells were stimulated with Poly(I:C)/TNF-α in vitro to assessthymic stromal lymphopoietin (TSLP), interleukin (IL)-33 and nuclear factor-κB (NF-κB) levels or expressions by immunofluorescence, enzyme linked immunosorbent assay (ELISA) and western blot. In the initial stage of AD, ear swelling and infiltration of inflammatory cells in ear tissues were markedly attenuated with YPFS treatments. The damaged structures of ear epithelium and the increased levels of Th2-cytokines induced by FITC were significantly rescued in YPFS-treated mice. The production of pro-allergic cytokines, TSLP and IL-33, as well as the cell populations of their target cells DCs and ILC2s were decreased in AD model, respectively. Likewise, the levels of TSLP and IL-33 in Poly(I:C)/TNF-α-stimulated HaCaT cells showed the same results. Lower levels of p-NF-κB were detected with YPFS treatment, and the expressions of TSLP and IL-33 could be further decreased with inhibiting of NF-κB. Therefore, YPFS attenuates allergic inflammation in the initial stage of AD probably through regulating NF-κB-TSLP/IL-33 pathway, which may provide a novel effective target for the prevention and treatment of allergic diseases.
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http://dx.doi.org/10.1016/S1875-5364(19)30074-3DOI Listing
July 2019